VIOLIN: Vaccine Investigation and Online Information Network

Babesia bovis

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. 11C5 (Protective antigen)
2. 12D3 (Protective antigen)
3. 21B4 (Protective antigen)
4. bov57 (Protective antigen)
5. msa (Protective antigen)
6. MSA-1 (Protective antigen)
7. RAP-1 (Protective antigen)
8. trap (Protective antigen)
Vaccine Information
1. B. bovis GST-12D3
2. B. bovis vaccine by Local veterinary institutes
References

I. General Information

1. NCBI Taxonomy ID:

5865

2. Disease:

Babesiosis, Texas Cattle Fever

3. Introduction

Babesia bovis is a single-celled protozoan parasite of cattle which occasionally infects humans. It is a member of the phylum Apicomplexa, which also includes the malaria parasite. The disease it and other members of the genus Babesia causes is a hemolytic anemia known as Babesiosis and colloquially called Texas Cattle Fever, Redwater or Piroplasmosis. It is transmitted by bites from infected larval ticks of the order Ixodida. It was eradicated from the United States by 1943, but is still present in Mexico and much of the world's tropics. The chief vector of Babesia species is the southern cattle fever tick Rhipicephalus microplus (formerly Boophilus microplus).

Babesia bovis are transmitted from mother ticks to their eggs, and can remain resident in tick populations for up to four years without infecting a vertebrate host. More commonly, a larval tick feeds upon a domestic cow, an African buffalo or a water buffalo, releasing the parasites into the animal's bloodstream. The involvement of the larval stage of the ticks in the cycle is specific to B. bovis; other Babesia species only involve adult ticks. The parasites then invade individual red blood cells, multiplying and destroying the host cells until the animal is stricken with hemolytic anemia. Adult ticks which feed upon infected cattle are infected themselves, completing the cycle. In humans, infection is rare and usually only affects splenectomized patients (Wiki: Babesia bovis).

II. Vaccine Related Pathogen Genes

1. 11C5

Gene Name : 11C5
Sequence Strain (Species/Organism) : Babesia bovis T2Bo
NCBI Protein GI : 156086656
Locus Tag : BBOV_IV008150
Protein Accession : XP_001610737
Taxonomy ID : 484906
Chromosome No : 4
Gene Starting Position : 946834
Gene Ending Position : 948172
Gene Strand (Orientation) : +
Protein pI : 5.11
Protein Weight : 36321.83
Protein Length : 346

DNA Sequence : Show Sequence

>NW_001820857.1:946834-948172 Babesia bovis T2Bo chromosome 4 gcontig_1104837696308, whole genome shotgun sequence
TGGCTGACGTCTACCGTCATTTTGACATGCACTGCGTTGCAGCTCGTGCATAGCTTTCGAGTGTTTCATC
TGGGTAAGGGTACAACGTTTTGTCGCGTCAACGTTAAGGCGCATTTCTTCTGATTTTAGCAAGAACGTCG
ACTATATTAAATTCACTTGGTCGGCTTCTTGTGACGCCTACTATTGCTGCGAGAGTGTGTAGGTTTTTCC
TTCAGGCATTACTCTGGAAAATACTATTCGACATACAAGATGTTGGCTACACGTTTTATTTTTAGTCTTT
GCCTATTTGTGGCTCATCAAGGCGTCAACGCCTTTTTCTATCGTGCAGTTCCTATGTACGATCAGAATGG
TAAGCTGGTTCCGGATTCCTCTAATATAGGTCCTTCATCCTTACGAATCGAGGCTACTGGTGTTGGTAGG
ATTTGTTCTACTATGCAATCAGGTATCTATCTCATCAATCCTTACGCTCAGCGCTGCCCAATTGGGAAGA
GCAAATGTGGACCTGAGCATGATTCCACCCTAGGAAAGTGTGCCATGTACTCTGGTTGTTACACCATCAG
TAACGACTTCCGTGCTTGCTCATGCTTGCCTGATTACTTCGGCAACCCTTACAAGCAATGCTTCAGACAC
TGTGACACGGATTTGGACTGCCCTTCACCTTATGCTGAGTGCAGGATGGACGCAGGCGAGACGATCAAGC
GCTGCAAGTGCAGAGCAGGTTGCCCAGGTGATGGTGTCATTTGCAAGCCCGATAAGATCTGTACTGACTT
GGCTGAAGACTCTGAGGCTCACCGTAGGTGCCTTCAGACCGGTATCACCGACAAGACATACGTTTGTGAT
GACGGTTATTACTTGGACACTGATAACAAGTGCCAGCCGATTGTTGAGCTTGAAGATGATAGGGTTGTGA
GCGTAATTGCCAGTGGCTACGTAAACGGCAGCCGTATTGATATTGGTAACTGCTTTTCAATTGAAATTAA
CAAAGACAACGGTATGTCTTACTTCTACCAACTTAACAGTGGTGACCCCGTTGTGATTGACCAAAAAATT
AAGACCGACGACCAGTTGATGTTGTTGTTCGAGGTTAAGGCTAATGACATTATTGCCTTTACCACTACTG
AGGCCAACCCTTCTGGTCTCACTAAAATCTTCACTATCTCAAATGGTCTTAAAGGCTGCAAAATCGACAG
TGTTATGAAGTACTCCCCAACCGGAGCTAAGGAGCACCCCTCAGGTGTCAGGGTTGAGGTTAAAGAGCTT
GAAACTTCGAGCGAAAGGCAGGAGCTTTAGTCAAAATGACGAACCGACTCAAATAGAGTTTAATTTTTTG
TAGAAAATT

Protein Sequence : Show Sequence

>XP_001610737.1 12D3 antigen [Babesia bovis T2Bo]
MLATRFIFSLCLFVAHQGVNAFFYRAVPMYDQNGKLVPDSSNIGPSSLRIEATGVGRICSTMQSGIYLIN
PYAQRCPIGKSKCGPEHDSTLGKCAMYSGCYTISNDFRACSCLPDYFGNPYKQCFRHCDTDLDCPSPYAE
CRMDAGETIKRCKCRAGCPGDGVICKPDKICTDLAEDSEAHRRCLQTGITDKTYVCDDGYYLDTDNKCQP
IVELEDDRVVSVIASGYVNGSRIDIGNCFSIEINKDNGMSYFYQLNSGDPVVIDQKIKTDDQLMLLFEVK
ANDIIAFTTTEANPSGLTKIFTISNGLKGCKIDSVMKYSPTGAKEHPSGVRVEVKELETSSERQEL

Molecule Role : Protective antigen
Molecule Role Annotation : Crude extracts of Babesia bovis parasites were systematically fractionated and tested in numerous sequential vaccination/challenge experiments in adult cattle. Two of these, GST-12D3 and GST-11C5, when used in combination were almost as protective as has been previously shown for the commercially available live attenuated vaccine (Wright et al., 1992).

2. 12D3

Gene Name : 12D3
Sequence Strain (Species/Organism) : Babesia bovis T2Bo
VO ID : VO_0011027
NCBI Gene ID : 5478971
NCBI Protein GI : 156086656
Locus Tag : BBOV_IV008150
Genbank Accession : AAXT01000002
Protein Accession : XP_001610737
Taxonomy ID : 484906
Chromosome No : 4
Gene Starting Position : 946834
Gene Ending Position : 948172
Gene Strand (Orientation) : +
Protein pI : 5.11
Protein Weight : 36321.83
Protein Length : 346

DNA Sequence : Show Sequence

>NW_001820857.1:946834-948172 Babesia bovis T2Bo chromosome 4 gcontig_1104837696308, whole genome shotgun sequence
TGGCTGACGTCTACCGTCATTTTGACATGCACTGCGTTGCAGCTCGTGCATAGCTTTCGAGTGTTTCATC
TGGGTAAGGGTACAACGTTTTGTCGCGTCAACGTTAAGGCGCATTTCTTCTGATTTTAGCAAGAACGTCG
ACTATATTAAATTCACTTGGTCGGCTTCTTGTGACGCCTACTATTGCTGCGAGAGTGTGTAGGTTTTTCC
TTCAGGCATTACTCTGGAAAATACTATTCGACATACAAGATGTTGGCTACACGTTTTATTTTTAGTCTTT
GCCTATTTGTGGCTCATCAAGGCGTCAACGCCTTTTTCTATCGTGCAGTTCCTATGTACGATCAGAATGG
TAAGCTGGTTCCGGATTCCTCTAATATAGGTCCTTCATCCTTACGAATCGAGGCTACTGGTGTTGGTAGG
ATTTGTTCTACTATGCAATCAGGTATCTATCTCATCAATCCTTACGCTCAGCGCTGCCCAATTGGGAAGA
GCAAATGTGGACCTGAGCATGATTCCACCCTAGGAAAGTGTGCCATGTACTCTGGTTGTTACACCATCAG
TAACGACTTCCGTGCTTGCTCATGCTTGCCTGATTACTTCGGCAACCCTTACAAGCAATGCTTCAGACAC
TGTGACACGGATTTGGACTGCCCTTCACCTTATGCTGAGTGCAGGATGGACGCAGGCGAGACGATCAAGC
GCTGCAAGTGCAGAGCAGGTTGCCCAGGTGATGGTGTCATTTGCAAGCCCGATAAGATCTGTACTGACTT
GGCTGAAGACTCTGAGGCTCACCGTAGGTGCCTTCAGACCGGTATCACCGACAAGACATACGTTTGTGAT
GACGGTTATTACTTGGACACTGATAACAAGTGCCAGCCGATTGTTGAGCTTGAAGATGATAGGGTTGTGA
GCGTAATTGCCAGTGGCTACGTAAACGGCAGCCGTATTGATATTGGTAACTGCTTTTCAATTGAAATTAA
CAAAGACAACGGTATGTCTTACTTCTACCAACTTAACAGTGGTGACCCCGTTGTGATTGACCAAAAAATT
AAGACCGACGACCAGTTGATGTTGTTGTTCGAGGTTAAGGCTAATGACATTATTGCCTTTACCACTACTG
AGGCCAACCCTTCTGGTCTCACTAAAATCTTCACTATCTCAAATGGTCTTAAAGGCTGCAAAATCGACAG
TGTTATGAAGTACTCCCCAACCGGAGCTAAGGAGCACCCCTCAGGTGTCAGGGTTGAGGTTAAAGAGCTT
GAAACTTCGAGCGAAAGGCAGGAGCTTTAGTCAAAATGACGAACCGACTCAAATAGAGTTTAATTTTTTG
TAGAAAATT

Protein Sequence : Show Sequence

>XP_001610737.1 12D3 antigen [Babesia bovis T2Bo]
MLATRFIFSLCLFVAHQGVNAFFYRAVPMYDQNGKLVPDSSNIGPSSLRIEATGVGRICSTMQSGIYLIN
PYAQRCPIGKSKCGPEHDSTLGKCAMYSGCYTISNDFRACSCLPDYFGNPYKQCFRHCDTDLDCPSPYAE
CRMDAGETIKRCKCRAGCPGDGVICKPDKICTDLAEDSEAHRRCLQTGITDKTYVCDDGYYLDTDNKCQP
IVELEDDRVVSVIASGYVNGSRIDIGNCFSIEINKDNGMSYFYQLNSGDPVVIDQKIKTDDQLMLLFEVK
ANDIIAFTTTEANPSGLTKIFTISNGLKGCKIDSVMKYSPTGAKEHPSGVRVEVKELETSSERQEL

Molecule Role : Protective antigen
Molecule Role Annotation : Crude extracts of Babesia bovis parasites were systematically fractionated and tested in numerous sequential vaccination/challenge experiments in adult cattle. Two of these, GST-12D3 and GST-11C5, when used in combination were almost as protective as has been previously shown for the commercially available live attenuated vaccine (Wright et al., 1992).
Related Vaccine(s): B. bovis GST-12D3

3. 21B4

Gene Name : 21B4
Sequence Strain (Species/Organism) : Babesia bovis
VO ID : VO_0011028
NCBI Protein GI : 1246924
Taxonomy ID : 5865
Gene Strand (Orientation) : ?
Protein Name : 21B4
Protein pI : 4.48
Protein Weight : 11838.99
Protein Length : 153

Protein Sequence : Show Sequence

>CAA01877.1 21B4, partial [Babesia bovis]
EFLREVPHTTMKVLNENIAQPAKEIIHEFGSGAKNFIWAAHEGTKQFLNETVGQPTREFLNGALETTKDA
LHHLGKSSEEANIYDATENTTEANDSTTSNGEDTAGYL

Molecule Role : Protective antigen
Molecule Role Annotation : Crude extracts of Babesia bovis parasites were systematically fractionated and tested in numerous sequential vaccination/challenge experiments in adult cattle. A short fragment of the third antigen (21B4) has been shown to be protective (Wright et al., 1992).

4. bov57

Gene Name : bov57
Sequence Strain (Species/Organism) : Babesia bovis
NCBI Protein GI : BBA45742
Taxonomy ID : 5865
Protein Name : BOV57
Protein pI : 4.62
Protein Weight : 52719.86
Protein Length : 549

Protein Sequence : Show Sequence

>BBA45742.1 BOV57, partial [Babesia bovis]
LLVHLVSINAFDLGEWSHDAHDTHDIKAHHESAADATPGAPAQALSESEEMEKALKALEEETKLENKPND
TPTPVPVTPSAEEKQDAPVEENKKVDQPKIEIPALHPPDSPLHTDKDDALDITTAPFTLVEDPASHENEL
TSEIPQSPADDTNVNAGNEDSIITDTTPIAKSMRLNTVTKIDETIEKLNHRFQTFLESVASSAHDLTYYQ
SLLDTAYDVFCREINGDMSPMGSGGQLDKDGNGVTLMISAEMSSAIRRSFDTKVEVLELAASEVASQKSK
EVGAQTIHDALTVGLRTVRDTITSPGMTIHTTSNDMKNMTAIVADMSKGLLADIIKWTLKEDVLKKRLFD
KIVERDNFIKTSPDELRMEAFTHAIRELAGEFHNAQKEKTGAIERQNGFKEYMDEMREDINTIQRLIDTY
FATVHKGHAKAILYEASKELRKDGNAESQLRLRVAEQKVHQEELKKAEPKQEDTGCPYPSVPEDPRRPIS
FDNPCVF

Molecule Role : Protective antigen
Molecule Role Annotation : (Ishizaki et al., 2017)

5. msa

Gene Name : msa
Sequence Strain (Species/Organism) : Babesia bovis
NCBI Protein GI : ABA06476
Other Database IDs : CDD:289042
Taxonomy ID : 5865
Protein Name : merozoite surface antigen 2
Protein pI : 4.38
Protein Weight : 28638
Protein Length : 326
Protein Note : Merozoite surface antigen 2c; pfam12238

Protein Sequence : Show Sequence

>ABA06476.1 merozoite surface antigen 2 [Babesia bovis]
MVSFNIITVAFCSILFNYAVASPQEEAVPTKQVNGSHLLFDDMKMLYDVMRSIDESMLKSILEKNFEAVG
MEATSATKTHDALKAVKQLIKTDAPFNTSDFDTLDLEYLSGQSNEELLKLLIEAIYGMEIIIEKTNSFVG
ESAKHSDKLDTDLRQYYWDNIYDDQSEYNKDKLSNLYKAFITDSGALRIASEELIKFETRKAQKDDYRFI
NPSSTSEAETPSPSSGENTAAQPPKPAETPKPTGSSFTYGGLTVATLCYFVLSAF

Molecule Role : Protective antigen
Molecule Role Annotation : (Antonio et al., 2010)

6. MSA-1

Gene Name : MSA-1
Sequence Strain (Species/Organism) : Babesia bovis
NCBI Protein GI : ABR92034
Other Database IDs : CDD:289042
Taxonomy ID : 5865
Protein Name : merozoite surface antigen 1
Protein pI : 5.22
Protein Weight : 32565.89
Protein Length : 381
Protein Note : Merozoite surface antigen 2c; pfam12238

Protein Sequence : Show Sequence

>ABR92034.1 merozoite surface antigen 1 [Babesia bovis]
MATFALFISALCCVLAITSAGEELTQSDVRNADTSIVPPEGSFYDDMSKFYGAVGSFDQTKLYSVLSANF
KAAKMDDQKVKDTFKNLYKVNALIKNNPMIRPDLFNATIVSGFSTKNDEEKFNAIFDSIKGMYYRAQHMD
KYLKSLRWNTDIVEEDREKAVEYFKKHVYTGEHVVDVNGMAGVCKEFLSPASDFYKLVESFDAFAHAKVH
AQVGNFVKPGTDIAPPKDVTDALEKELQEQKPARSESTEVPAPGDASGVQQPPASGTSPQGPAPTTPSPS
PESSGNLQGQQGTTKPAGSSFTYGGLTVATLCYFVLSAF

Molecule Role : Protective antigen
Molecule Role Annotation : (Antonio et al., 2010)

7. RAP-1

Gene Name : RAP-1
Sequence Strain (Species/Organism) : Babesia bovis T2Bo
VO ID : VO_0011026
NCBI Gene ID : 5479143
NCBI Protein GI : 156087004
Locus Tag : BBOV_IV009870
Genbank Accession : AAXT01000002
Protein Accession : XP_001610909
Taxonomy ID : 484906
Chromosome No : 4
Gene Starting Position : 1308099
Gene Ending Position : 1310097
Gene Strand (Orientation) : +
Protein pI : 7.16
Protein Weight : 61365.28
Protein Length : 565

DNA Sequence : Show Sequence

>NW_001820857.1:1308099-1310097 Babesia bovis T2Bo chromosome 4 gcontig_1104837696308, whole genome shotgun sequence
ACTATTGTTTGTATCTTTCGACGGATAGTATTTTACATATACATTTGTCGGCTTTTATATATAGCAGTGC
TATAGACAAACAATACACAGATTAATCTTTAGATACTAAGTTCAATAATATTACGGACATATTGTAGACA
ATGAGAATCATTAGCGGCGTTGTCGGTTGCCTTTTCTTGGTGTTTTCACACCATGTGTCTGCTTTTCGCC
ACAATCAGAGAGTAGGAAGTCTCGCTCCAGCTGAAGTGGTAGGTGATTTAACCTCCACATTGGAAACAGC
TGATACTTTGATGACTCTCCGTGACCACATGCACAACATTACTAAGGATATGAAACATGTTTTGAGCAAT
GGCCGTGAGCAGATTGTAAATGATGTTTGCTCTAATGCTCCTGAGGACTCCAACTGTCGTGAGGTAGTTA
ACAATTATGCTGACCGTTGTGAAATGTACGGATGCTTTACGATTGACAATGTCAAATATCCGTTGTATCA
AGAGTACCAACCTCTATCTCTTCCAAACCCTTACCAGTTGGATGCTGCGTTCAGATTGTTCAAAGAGAGT
GCATCCAATCCTGCTAAGAACAGCGTAAAACGCGAATGGTTGCGTTTCAGAAATGGAGCGAACCATGGTG
ATTACCACTACTTCGTCACTGGTCTGTTGAACAACAATGTTGTGCACGAGGAAGGAACTACCGATGTTGA
ATATCTTGTCAACAAGGTACTCTATATGGCTACCATGAACTACAAGACTTATTTGACAGTAAACAGTATG
AACGCCAAGTTTTTCAACAGATTCAGCTTCACTACAAAGATATTCAGCCGTCGTATTAGGCAAACATTGA
GTGATATCATCAGGTGGAATGTTCCTGAAGATTTTGAAGAAAGGAGCATCGAACGTATCACTCAACTTAC
TAGCAGCTACGAAGATTACATGTTGACCCAGATTCCAACTCTTTCCAAGTTTGCACGTCGTTATGCTGAC
ATGGTGAAGAAGGTTCTGCTCGGTAGCTTGACCTCGTACGTTGAAGCTCCTTGGTACAAAAGATGGATAA
AGAAATTCAGAGACTTTTTCTCTAAAAACGTTACCCAACCTACAAAGAAGTTCATCGAGGATACTAACGA
AGTTACCAAAAACTATCTGAAAGCCAATGTTGCTGAGCCCACTAAAAAGTTTATGCAGGACACTCACGAA
AAAACCAAAGGCTATCTGAAAGAGAATGTAGCCGAACCTACTAAGACTTTTTTCAAGGAGGCTCCTCAAG
TCACCAAACACTTCTTCGATGAGAACATTGGCCAACCCACCAAGGAGTTTTTCAGGGAAGCTCCCCAAGC
CACTAAACATTTCCTAGACGAAAACATCGGTCAACCAACCAAGGAGTTCTTCAGGGAGGCTCCCCAAGTC
ACTAAGCACTTCTTAGACGAGAATATTGCTCAACCTACTAAAGAATTTTTCAGGGATGTCCCTCAAGTCA
CCAAGAAGGTTATAACTGAGAACATTGCTCAACCAACTAAGGAATTCCTTAAGGAGGTTCCTCATACTAC
CATGAAAGTCTTGAATGAAAACATTGCTCAACCTGCCAAGGAAATCATACATGAGTTTGGTACTGGCGCC
AAGAATTTCATTTCCGCAGCCCATGAAGGTACTAAGCAGTTCTTAAACGAAACTGTTGGCCAACCTACAA
AGGAATTCCTGAACGGAGCTCTAGAAACTACTAAAGACGCATTACACCATCTGGGTAAATCATCAGACGA
AGCCAACATTTATGATGCCTCGGAAAATACCACTCAGTCTAACGACTCAACTACTTCCAACGGTGAAGAC
ACCGCCGGATACCTCTGATGAGATGCGTTTATAATGGCACAAACTCAGCAAATGATGTATCGTCATCTGA
TCCATCGGTTTTCAATATTGTATTGGATGCAATATCTGAATGCATACGATGCGACAGTTTCCATCATCGG
GTGCCGAATCGTAACTCTCAAAACACCATTTTAAGTTAT

Protein Sequence : Show Sequence

>XP_001610909.1 rhoptry-associated protein 1 (RAP-1) [Babesia bovis T2Bo]
MRIISGVVGCLFLVFSHHVSAFRHNQRVGSLAPAEVVGDLTSTLETADTLMTLRDHMHNITKDMKHVLSN
GREQIVNDVCSNAPEDSNCREVVNNYADRCEMYGCFTIDNVKYPLYQEYQPLSLPNPYQLDAAFRLFKES
ASNPAKNSVKREWLRFRNGANHGDYHYFVTGLLNNNVVHEEGTTDVEYLVNKVLYMATMNYKTYLTVNSM
NAKFFNRFSFTTKIFSRRIRQTLSDIIRWNVPEDFEERSIERITQLTSSYEDYMLTQIPTLSKFARRYAD
MVKKVLLGSLTSYVEAPWYKRWIKKFRDFFSKNVTQPTKKFIEDTNEVTKNYLKANVAEPTKKFMQDTHE
KTKGYLKENVAEPTKTFFKEAPQVTKHFFDENIGQPTKEFFREAPQATKHFLDENIGQPTKEFFREAPQV
TKHFLDENIAQPTKEFFRDVPQVTKKVITENIAQPTKEFLKEVPHTTMKVLNENIAQPAKEIIHEFGTGA
KNFISAAHEGTKQFLNETVGQPTKEFLNGALETTKDALHHLGKSSDEANIYDASENTTQSNDSTTSNGED
TAGYL

Molecule Role : Protective antigen
Molecule Role Annotation : RAP-1 (21B4) is a candidate for inclusion in a B. bovis vaccine based on its ability to induce partial protection in vaccinates, defined as a significant reduction in parasitemia upon challenge (Wright et al., 1992).

8. trap

Gene Name : trap
Sequence Strain (Species/Organism) : Babesia bovis
NCBI Protein GI : ACM44016
Other Database IDs : CDD:294047
CDD:238119
CDD:214559
Taxonomy ID : 5865
Protein Name : thrombospondin-related anonymous protein
Protein pI : 4.26
Protein Weight : 68214.12
Protein Length : 746
Protein Note : Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of...; cl00057

Protein Sequence : Show Sequence

>ACM44016.1 thrombospondin-related anonymous protein, partial [Babesia bovis]
MIGYIKILASVPLLSLAFLATTGIHAFADKGIGSPKGKQCKKQLDFSIVVDESASISNDQWEGQMIPFLR
NLIHTVDLDNTDIRLSLTTYSTPTRQIFTFLDAAASSTRLALTKLDWMAGTKARSGMTYTGRALNYVRKA
ILPYGRKNVPKALLLITDGVSSDGSYTAQVAAMLRDEGVNVMVIGVGDVNVAECRGIVGCDGVMDCPMFK
HTNWKDIMGLFNSLMKEVCDILPQDAVCEPVWAEWSSCKGECGVPGTRTRALLDLRMIEKPVSGSNGQPG
KSCEDQKMNFLPQSETCTIECNHEPVPSSPVPVPHDMDNPEPSRATPEGDLDHSHSSIPSTPDMSSSSTD
MPSSSSDMPSSSSDMPSSTTDMPSSSSDMPSSSSDMPSSSSDMPSSTTDMPSSTTNMSSSSSDMPSSTTD
MSSSPTHTRVEETDEEHNHRKDMDMKFPENMDDIPVEDIPMPIDPRHGVEPSASDVIPEDDQLRKKLEMQ
REEDLRKELMLQHELKLQEEKERAAILENNTPYGSATSVSQGGESTTGLPQSSEADAISHDVYDNHPEES
ENTGINAEVTESEDYEGEKQNDESNARSTSNTTKIAGGALLGLLLLGAGGGYAMYKKNKTPTVETESGDY
TGADESAEPMKEGDTYTVTEFDNNIWG

Molecule Role : Protective antigen
Molecule Role Annotation : (Terkawi et al., 2013)

III. Vaccine Information

1. B. bovis GST-12D3

a. Vaccine Ontology ID:

VO_0011375

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

B. bovis T2Bo 12D3

e. Gene Engineering of 12D3

Type: Recombinant protein preparation
Description: The 12D3 protective antigen was cloned and expressed as a glutathione-S-transferase (GST) fusion protein (Wright et al., 1992).
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0000139

g. Vector:

pGEX

h. Immunization Route

Intramuscular injection (i.m.)

i. Cattle Response

Vaccination Protocol: 50 micrograms of the GST-12D3 fusion protein was mixed with FCA and used to vaccinate cattle (Wright et al., 1992).
Challenge Protocol: Challenged with virulent B.bovis (Wright et al., 1992).
Efficacy: Immunised animals had parasitaemias one order of magnitude lower than controls when challenged with a heterologous strain of B. bovis. In a series of experiments, 100% of controls were treated, whereas only 16% of the GST-12D3 vaccinated animals required treatment (Wright et al., 1992).

2. B. bovis vaccine by Local veterinary institutes

a. Manufacturer:

Local veterinary institutes

b. Vaccine Ontology ID:

VO_0001033

c. Type:

Live, attenuated vaccine

d. Status:

Research

e. Host Species for Licensed Use:

Cattle

f. Immunization Route

g. Description

Infected blood from splenectomized calves(de and Combrink, 2006). Host species also include Babesia bigemina.

IV. References

1. Antonio et al., 2010: Antonio Alvarez J, Lopez U, Rojas C, Borgonio VM, Sanchez V, Casta�eda R, Vargas P, Figueroa JV. Immunization of Bos taurus steers with Babesia bovis recombinant antigens MSA-1, MSA-2c and 12D3. Transboundary and emerging diseases. 2010; 57(1-2); 87-90. [PubMed: 20537116].

2. Brown et al., 1993: Brown WC, Palmer GH, McElwain TF, Hines SA, Dobbelaere DA. Babesia bovis: characterization of the T helper cell response against the 42-kDa merozoite surface antigen (MSA-1) in cattle. Experimental parasitology. 1993; 77(1); 97-9110. [PubMed: 8344411].

3. Brown et al., 1996: Brown WC, McElwain TF, Ruef BJ, Suarez CE, Shkap V, Chitko-McKown CG, Tuo W, Rice-Ficht AC, Palmer GH. Babesia bovis rhoptry-associated protein 1 is immunodominant for T helper cells of immune cattle and contains T-cell epitopes conserved among geographically distant B. bovis strains. Infection and immunity. 1996; 64(8); 3341-3350. [PubMed: 8757873].

4. de and Combrink, 2006: de Waal DT, Combrink MP. Live vaccines against bovine babesiosis. Veterinary parasitology. 2006; 138(1-2); 88-96. [PubMed: 16504404].

5. Ishizaki et al., 2017: Ishizaki T, Sivakumar T, Hayashida K, Takemae H, Tuvshintulga B, Munkhjargal T, Guswanto A, Igarashi I, Yokoyama N. Babesia bovis BOV57, a Theileria parva P67 homolog, is an invasion-related, neutralization-sensitive antigen. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 2017; 54; 138-145. [PubMed: 28668608].

6. Norimine et al., 2004: Norimine J, Mosqueda J, Palmer GH, Lewin HA, Brown WC. Conservation of Babesia bovis small heat shock protein (Hsp20) among strains and definition of T helper cell epitopes recognized by cattle with diverse major histocompatibility complex class II haplotypes. Infection and immunity. 2004; 72(2); 1096-1106. [PubMed: 14742557].

7. Terkawi et al., 2013: Terkawi MA, Ratthanophart J, Salama A, AbouLaila M, Asada M, Ueno A, Alhasan H, Guswanto A, Masatani T, Yokoyama N, Nishikawa Y, Xuan X, Igarashi I. Molecular characterization of a new Babesia bovis thrombospondin-related anonymous protein (BbTRAP2). PloS one. 2013; 8(12); e83305. [PubMed: 24349483].

8. Wiki: Babesia bovis: Babesia bovis [http://en.wikipedia.org/wiki/Babesia_bovis]

9. Wright et al., 1992: Wright IG, Casu R, Commins MA, Dalrymple BP, Gale KR, Goodger BV, Riddles PW, Waltisbuhl DJ, Abetz I, Berrie DA. The development of a recombinant Babesia vaccine. Veterinary parasitology. 1992; 44(1-2); 3-13. [PubMed: 1441189].