Mycoplasma gallisepticum |
Table of Contents |
- General Information
- NCBI Taxonomy ID
- Disease
- Introduction
- Microbial Pathogenesis
- Host Ranges and Animal Models
- Vaccine Related Pathogen Genes
- gapA
(Protective antigen)
- TM-1
(Protective antigen)
- lpd
(Virmugen)
- Vaccine Related Host Genes
- IgG Fc fragment
- Vaccine Information
- Avian Encephalomyelitis-Fowl Pox-Mycoplasma Gallisepticum Live Fowl Pox Vector, Live Virus Vaccine (USDA: )
- Fowl Pox-Mycoplasma Gallisepticum Live Fowl Pox Vector Vaccine (USDA: 1D51.R0)
- M. gallisepticum TM-1 Protein Subunit Vaccine
- Mycoplasma Gallisepticum Live Culture Vaccine (USDA: 1751.00)
- Mycoplasma Gallisepticum Live Culture Vaccine (USDA: 1751.01)
- Mycoplasma gallisepticum lpd mutant vaccine
- Newcastle-Bronchitis Mass Type, Killed Virus Vaccine-Mycoplasma Gallisepticum Bacterin (USDA: 48B5.10)
- Newcastle-Bronchitis Mass Type, Killed Virus Vaccine-Mycoplasma Gallisepticum Bacterin (USDA: 48B6.10)
- Porcine Rotavirus Modified Live Virus Vaccine-Clostridium Perfringens Type C-Escherichia Coli Bacterin-Toxoid (USDA: 49C1.21)
- Porcine Rotavirus Modified Live Virus Vaccine-Escherichia Coli Bacterin (USDA: 49K1.20)
- Porcine Rotavirus-Transmissible Gastroenteritis Modified Live Virus Vaccine-Bordetella Bronchiseptica-Clostridium Perfringens Type C-Erysipelothrix Rhusiopathiae-Escherichia Coli-Pasteurella Multocida Bacterin-Toxoid (USDA: 49T9.21)
- Porcine Rotavirus-Transmissible Gastroenteritis Modified Live Virus Vaccine-Clostridium Perfringens Type C-Escherichia Coli Bacterin-Toxoid (USDA: 49B1.21)
- Porcine Rotavirus-Transmissible Gastroenteritis Modified Live Virus Vaccine-Escherichia Coli Bacterin (USDA: 49P1.20)
- Vaxsafe MG
- References
|
I. General Information |
1. NCBI Taxonomy ID: |
2096 |
2. Disease: |
Chronic respiratory disease |
3. Introduction |
Mycoplasma gallisepticum (MG) is a bacterium belonging to the class Mollicutes and the family Mycoplasma. It is the causative agent of chronic respiratory disease (CRD) in chickens and infectious sinusitis in turkeys, chickens, game birds, pigeons, and passerine birds of all ages. MG is transmitted through the eggs of carrier hens. Most commercial flocks are MG free, however, and the disease is most frequently introduced from carrier birds being brought into the flock. Stress is thought to lower the poults resistance to the disease, like in CRD. Symptoms of infectious sinusitis for turkeys include watery eyes and nostrils and the area under the eye may be swollen. One may find airsacculitis with yellow exudates in the air sacs. Chickens may have no outward symptoms or there may be a sticky nasal discharge, airsacculitis, coughing, difficult breathing, swelling of the face, sneezing, a foamy secretion in the eyes, and a drop in food consumption. Egg production may be far below normal and there may be a drop in body weight as well. In CRD of young poultry, symptoms include rattling, sniffling, and sneezing. In older birds, conditions may go unnoticed. Worse conditions may exhibit nasal discharge or foamy secretion from the eyes. Lack of appetite, trouble breathing and a drop in egg production are also symptoms.The birds will also be stunned and unthrifty (Wiki: Mycoplasma gallisepticum). |
4. Microbial Pathogenesis |
The epithelium of the upper air passages is most susceptible to infection; however, in severe, acute disease the infection is also found in the lower respiratory tract. There is a marked interaction between respiratory viruses, Escherichia coli , and M. gallisepticum in the pathogenesis of chronic respiratory disease. Once infected, birds remain carriers for life (Merck Vet Manual: M. gallisepticum). |
5. Host Ranges and Animal Models |
M. gallisepticum infection is commonly designated as chronic respiratory disease in chickens and as infectious sinusitis in turkeys. Infection may also be seen in pheasants, chukar partridges, and peafowl. Infection in pigeons, quail, ducks, geese, and psittacine birds should be considered. Passerine-type birds are quite resistant, although M. gallisepticum is the major cause of natural outbreaks of conjunctivitis in wild house finches (Carpodacus mexicanus) in the eastern USA (Merck Vet Manual: M. gallisepticum). |
II. Vaccine Related Pathogen Genes |
1. gapA |
-
Gene Name :
gapA
-
Sequence Strain (Species/Organism) :
Mycoplasma gallisepticum
-
NCBI Protein GI :
AAS65542
-
Other Database IDs :
CDD:332876
-
Taxonomy ID :
2096
-
Protein Name :
GapA
-
Protein pI :
5.07
-
Protein Weight :
11427.9
-
Protein Length :
159
-
Protein Note :
Mycoplasma adhesin P1; cl28055
-
Protein Sequence : Show Sequence
>AAS65542.1 GapA, partial [Mycoplasma gallisepticum]
PNRITNPLMNRDNVIGQGAFISRNDIPSSFFENKINDIVTTEADGKEVLDSKYINSIYRYTPPQNNPDIR
LRLLVIDRSRATNDFIKLLPQVLVDGEYVAVPQ
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Wijesurendra et al., 2017)
|
2. lpd |
-
Gene Name :
lpd
-
Sequence Strain (Species/Organism) :
Mycoplasma gallisepticum str. R(low)
-
NCBI Gene ID :
1089767
-
NCBI Protein GI :
31544686
-
Locus Tag :
MGA_0161
-
Genbank Accession :
AE015450
-
Protein Accession :
NP_853264
-
Taxonomy ID :
710127
-
Gene Starting Position :
667939
-
Gene Ending Position :
669342
-
Gene Strand (Orientation) :
-
-
Protein Name :
dihydrolipoamide dehydrogenase (E3) component of pyruvate dehydrogenase complex
-
Protein pI :
7.54
-
Protein Weight :
47042.25
-
Protein Length :
467
-
Protein Note :
MGA_0161; Lpd [C] COG1249 Dihydrolipoamide dehydrogenase/glutathione oxidoreductase and related enzymes; PRK06416, dihydrolipoamide dehydrogenase; MGRL526
-
DNA Sequence : Show Sequence
>gi|294660180:667939-669342 Mycoplasma gallisepticum str. R(low) chromosome, complete genome
TTTAGTGTTTTTTGTTGAAGTGGTTATAAATTGCTTGTTTAGCACATTCTGAGATGATTTCAGCAATTGT
TGGGTGTGGGTGAATTGCTTGTTCTAATTCGAACACTGTTAGTTCATTTTCCATTGCTAAAGCAATTTCA
GAGATCATATCACTAGCAGTAGAAGCAATCAAGACACATCCTAAGATCTCACCATACTTCTTACCGAACA
TGAATTTAATGAATCCTTCAGTTGAACCATCAGCAATCGCTTTACCATTAACTGCCATTGGCATCTTAGA
AACCACATATTCAATGTTCTTTTCTTTTAATTGTTGTTCAGTATAACCAATTGTTGCGATCTCTGGGTAA
GTATAAATACATCCAGGAGTGGTTAGTGAACTTAATTTCTTTGTCTTACGATTTAAGATCGTATCAACAG
CATATAATGCTTGGTGGTACGCATAATGAGCTAGCATGATCTGACCAGCAGCATCACCAATCACATAGAT
TGAATCAGTTGAAGTTTGTAACTTGTCGTTTAATTTAATGCGGTTGCGATCATCTCTTGCTACGTTGAAT
GATCCAAAAACTTGATCATTAACTACCCGACCAACTGATTCTAGGATATATTGAGAAGGTAGTTGGTAAG
CTGTGCCATTATCTTCATAGATAATTGCGTTGTTTTGGTAACCTAAAATCTTGGCATTATAAACAACATT
AACGCCTAATGATTTCATGCGTTTAGCAATGAAATCACTTGCATCTTGATCTAATAATTCGCATAAACGA
TCAACAGCTTGTAAGATCGTTACTTTAGTACCTAATGAAGCGTATAACATCGCAAATTCGATCCCAATTA
CACCCCCACCGATCACAACTAATGATTGAGGGATTTGTGGTAATTCAAGTGCTTTAGTTGACTCAATGAT
CACACCTTCAGCACGACCTTGGTCAAACCCTGGAAGTGGTAATTTTCTTGGTGAAGAACCAGTGGCAACG
ATAATATCTTTTGTTGTAATGTAAAATGTTTGGTTGTTTACAGTTACTGTATGACCATCAACAATTCTAG
CTTCACCTACGATCGTATCAGCTTTAGCTACTTTTAAGATCGTTTTTACCCCGTTAACTAGCTTATTAAC
AACAGCTTCTTTTCTTTTTAAGATATTTGCTCAGTTAACAGATAATTGATTATTAACTAAATCTAGTCCG
TATTCAGCTGCTTTTTCAATGTAGTGTTTAACTTTAGAAGATTGTAGTAATGTTTTAGTTGGAATACAAC
CAACATTTAAACAAACCCCACCATAAGTTCCACGTTCAATTACTAAAGTTTTAAGACCGTTTTTTGCAGC
ATGTTCACCAGCTACATAACCACCAGGTCCAGCACCGATAACAATTAGATCGTAATTATACATATTAAGT
ACCA
-
Protein Sequence : Show Sequence
>gi|31544686|ref|NP_853264.1| dihydrolipoamide dehydrogenase (E3) component of pyruvate dehydrogenase complex [Mycoplasma gallisepticum str. R(low)]
MVLNMYNYDLIVIGAGPGGYVAGEHAAKNGLKTLVIERGTYGGVCLNVGCIPTKTLLQSSKVKHYIEKAA
EYGLDLVNNQLSVNWANILKRKEAVVNKLVNGVKTILKVAKADTIVGEARIVDGHTVTVNNQTFYITTKD
IIVATGSSPRKLPLPGFDQGRAEGVIIESTKALELPQIPQSLVVIGGGVIGIEFAMLYASLGTKVTILQA
VDRLCELLDQDASDFIAKRMKSLGVNVVYNAKILGYQNNAIIYEDNGTAYQLPSQYILESVGRVVNDQVF
GSFNVARDDRNRIKLNDKLQTSTDSIYVIGDAAGQIMLAHYAYHQALYAVDTILNRKTKKLSSLTTPGCI
YTYPEIATIGYTEQQLKEKNIEYVVSKMPMAVNGKAIADGSTEGFIKFMFGKKYGEILGCVLIASTASDM
ISEIALAMENELTVFELEQAIHPHPTIAEIISECAKQAIYNHFNKKH
-
Molecule Role :
Virmugen
-
Molecule Role Annotation :
An lpd mutant is highly attenuated in chickens and induces significant protection from challenge with wild type M. gallisepticum (Gates et al., 2008).
- Related Vaccine(s):
Mycoplasma gallisepticum lpd mutant vaccine
|
3. TM-1 |
-
Gene Name :
TM-1
-
Sequence Strain (Species/Organism) :
Mycoplasma gallisepticum
-
VO ID :
VO_0011178
-
NCBI Protein GI :
7249262
-
Other Database IDs :
CDD:283372
-
Taxonomy ID :
2096
-
Gene Strand (Orientation) :
?
-
Protein Name :
TM-1
-
Protein pI :
8.38
-
Protein Weight :
29230.13
-
Protein Length :
329
-
Protein Note :
Mycoplasma haemagglutinin; pfam05692
-
Protein Sequence : Show Sequence
>AAB28343.2 TM-1, partial [Mycoplasma gallisepticum]
MNKKRIILKTISLLGTTSFLSIGISSCMSITKKDANPNNGQTQLQAARMELTDLINAKARTLASLQDYAK
IEASLSSAYSEAETVNNNLNATLEQLKMAKTNLESAINQANTDKTTFDNEHPNLVEAYKALKTTLEQRAT
NLEGLASTAYNQIRNNLVDLYNNASSLITKTLDPLNGGMLLDSNEITTVNRNINNTLSTINEQKTNADAL
SNSFIKKVIQNNEQSFVGTFTNANVQPSNYSFVAFSADVTPVNYKYARRTVWNGDEPSSRI
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
An antiserum raised in chicken against the TM-1 polypeptide, which was produced by recombinant Escherichia coli cells and purified by column chromatography, inhibited growth of M.g. cells in vitro. Moreover, chickens immunized with this 29 kDa polypeptide (TM-1) were partially protected from a challenge with virulent M.g (Saito et al., 1993).
- Related Vaccine(s):
M. gallisepticum TM-1 Protein Subunit Vaccine
|
III. Vaccine Related Host Genes |
1. IgG Fc fragment |
-
Gene Name :
IgG Fc fragment
-
Sequence Strain (Species/Organism) :
Gallus gallus
-
NCBI Gene ID :
424904
-
Taxonomy ID :
9031
-
Chromosome No :
9
-
Gene Starting Position :
12628992
-
Gene Ending Position :
12700391
-
Gene Strand (Orientation) :
-
-
Protein Name :
Fc fragment of IgG binding protein
-
DNA Sequence : Show Sequence
>gi|358485503:12628992-12700391 Gallus gallus breed Red Jungle fowl, inbred line UCD001 chromosome 9, Gallus_gallus-4.0, whole genome shotgun sequence
TGCAATTATTTATTTATTATTCTGAAAGTGTTAGAAATATTATTAAAATACAAGAGCAGAAAGTAATACG
TATGAATTGGGGGGAAAAAAACAACACATACAGACACAGAAAAACACACTAACTACAGGTATGTACTTTG
GGGTTCATCTTGGTCAAATGCAGCGTTCACTACGTAGCCATAATTGTGTTCCCGTACTCTGTCACCTGAT
GAATCCTCAGACTCGTGCAAAGATGAATTCCGTGCATTGATGTTGGCATCTGCCTTTCTCCTCCTTCTGT
ACACAAACAAACAAACAAACACCAACAACCATCTTTAATTTCTTATCTGGTCAAACGGTGGTGTGAGTGT
GTGTGTCAGATTCCAAAGAATTCTAAGTTTTGTGGTAGTGGTGCATATGCATTATGTAATAGGAATGGCA
CATCTTCATTCCTGTAGAAGAAAGCTTTCCCTCGTGTTCCTTAAATGATATCTTGAACGCTGTACTTCCT
TTCTGATTCTGCAGGTGTGGGAATAGAGGAGTTTGGGGGTGTACAGAAGCCAAAATGAGTGTCTTGCCCT
TGCTGAATGTAGCAAAGCTGGGAATAGTCTTACCTGAACAGATACACTGCAGAGGTGATGAGAATGACAA
CGGCAACCACTGCTACTGTAACTCCAACAGCGATGGCAACTGTATTGCTCTGAGAACTCTCTGGGATACT
TGTGTCATCTGAAGGAGAAAGGAGCCCGGTCACTATAAGCTAAGAGCACTGAGAACAGAAGATTCTCCTC
AATTCCAAGTGTGAAACTGCACTGTGTGGAATCTGAACAGGAAGCAGACGTGCCTGTCTGCTTGGTTTAT
ACTGTGTTGTCTGACTTAACTGGCTTTCTGGAATGGTGTCAGACCAAGGTCCTGTATGCCCTGCAGAGCC
CTGCTGTCTTGCCTTCTAATACCTCTGTGCTGAGGACCTGCCCCCTTCTTTCTGTTTGAACATACCAGTA
CCAACATGACCACCCAGCTTGTGGTACAAGGCCCAGTTTTTACTTGAAGGAATAGTGCCACTGAATTGGT
TGGAAAGTGTAGTGGAATATTTCTAGTGTCAGCCTCCACTCTGCCCATGACTAAAATACAATCTGCCCTT
GAAAAGATGTGATGAGGTCCCAGACTGATCTGACACCCCCTCCCAGTGGGTTACTTATTGATTCACAGCA
TGGCAATTAAAAGCCAGCTCAGTCTTCTCCATAATGACACATTTTTTTTTTTTTATAGTGGTGTGTGTAA
TTCAGGCAGAGAAATCCAGAGTAGTTTTATTACCTATCCTTTCAATATTGCATCTTTGTCCCATGTACAG
CTCTGTACATTCACAGTAGTAGTTATCAGAGGTGTCATTGGTTGTTTCAGAGCAAATTCCATCATTCTGG
CAAGGACTTGGCATACACGGTCCACCTAACAAAAAGGAAGGAGAAACACATCAGAATCTCATGTAATCCA
GCCCTGAGAGAACTACTCTTGGTTAGATTACACTGCTACTGCTGGACGTGCCCTGTGTTTTAAGGCCTGT
TATTTATTTTGTACTTGATAACAGGAGTATGGGAAAGAAACAAAGTTCTCCAGTAAGCTTATAAAGAAAG
CTTATTATTTCTTCTGTTCAGACTGTTGCAACCCCAGACCCAGACACATCCTATAAGCAGTAACTCAAGA
CTTCTTAGCAGCGAAGGAAAACACGATAGGTTTAGTGGTTCACTTACTCCTGTAGCACCCACGAGTGTAG
TTTGCAATGTTACAGATTTCATCAGAACCACACAGTATGTTAGAGCAGGTGACAGTGGAGCTCTCTGTGC
ACTGGCATTTCTGGGAGCAGTCATCAGTGGTGAACACCTCTCCAATCTGGAAGATAGCAAGTGAATGTTA
AAAGATATCCAAATCAGGCAGGGAAACGTGAATGGAACACTGGAGCCCCATGCTACTCCGATTCTTCCTG
ATGAATGACAACATGGCAGATCTCTCTTTTTTGTACTTTGAATACAGAAAGCAAGCAAGCAGCTCATTAA
CCCATCTCCTTGAAACTCAAGGAATTTTGATGAAAATTTGAGGTGTGAGATCCCAGACCCAGGTTGGGTA
TATGGACAACCAACAATATCTAACTGTTGCAGGAGATAAAGTGGCAAATGCATCATATTGTGCCTCCAAG
ACGATGTGCAAAGGTGCCATGTGTCATTTCTCTGGCAAGCCCCATTTGTTTAGAGTGTCTACCTCCTCCC
ATAACACCACCTTTACTTGCTTTAGGCCTTCTCTGTATCAAACCTCCAGTGCTGAATTGACTACAAAGCT
TTTTATCCCACAGCTGGAAGGGTGGAATCTCTGGATTCCTCAGAATCCAGCCTTGCCGGAGGCTCTCTTC
ATGCTGCAGCCAGCATTACAGACTCTCAGCCCCTCCTGTGCCACTCTTCACTGAGAGGAGAGGGACCATG
GTGACCAGTAGAATTTTTCTATAACTGGCGTATTGTAAATTTTAGAGCCTATTGTGGCTGTTTACTGTAA
TAAGGGTGAAGAATTAACAAGGAGGTCAGAGTGTAATCATATGCTTCCCCCAAGTGCCTGCAGAGTATTC
TGACAGGCAGAGTTTCAGAGGCAGACTGACTCATGTATTCTCTCCTCCCTTCCTCCAGAGACCAGAATAT
TATTAAATAAATTGCTCTTTGTTTCTAATGCAAAGTTACATGCTGAGGAGAACAAAGAGATTATCAAAAT
AATTAGCACAAGAAAATATTGTTTAGCACTGCGGGAGCTGTCTGGCCTGCTGAGACACTGTACCTCATAA
TATTTGTCAAGGTATGTGCAGCCACATTCTCTGTAAGGCACACATTCAGAGTCACTAAGAACATAGCCAG
GGAGACATTCACAGCCTTCAACACAAGGCGATTCACACTCAGAGGGGCTGGTCATGTCGCTGCAGGATGC
TGGGCAAGCAGTCATGCATGAGTTGTATTTGCTGTTGGGTGGACACGACATCTCTGTAAGAATAAGGAGA
GTCCAGTTATAACAGGTTGCTGCTACACATTGCCTTTTCAGAGCTGTTTTGTAAGGGCAAAATGATGAAC
ACACATACATAGAAATGTTCACATGTATATAATGGTAATGAAGTTATAAGTGAATAACTTGAAAATAAGG
AAATGCATTCTCAAAGCTATGCATATGCAGTGCTCTGAGTCCAGGTGTTCACTAATATCAAATTACTTCT
CACACATACAGAGTAGCTATCACTGAAAGCATCAGAGTAGTTTCACAGCTCTGAGAAGTGCGTTTCATCA
GAAATACAAAATCCTCTGCCCATGGGTGATGCAGGGTAAGTATGGCCTGGATAAACCTCTCAGCTTGGGA
AAGAAAAGTTAGGAAAAAGAGGTTTGAGATTATTGTAAATCCTATTTTCACATTTTTCAGTCTGAAAGAC
GAGTTGGGAATGGAGACATTTTATTTTTCTCACCGAACTTCTCATGGCAGGGGAGCTGACCGCTCTGCTC
CCCTGAGCAGCTTTCTAAGGTGGAGTCTCATACAAAACATCAGCATAAGACTCAGAGGCAGTGACTGAAG
AGCAAGTGTGCGTGCTAAACTATGAACGATGATTGAAGTGTGAATCAAAGTTTGTAAGACCTAAGAAGCT
TTGACATACAGCTTCTAGCAAAAACCTCTAATCCCATATTTTGAGAGTCCTTTAGGCAGAAACCACACTT
TCAATTTCTTCCTGTACTTACCACAAGCTGTCTGTTGCCTCCAGCCTTCCATATTGACTCCTTGCTCCTG
ACAAGCCAGCACGTACTCCTTCAGATGGCGACATAACGTGTCAGCATCATCTTCTACACACATATCGAAG
ATGCAGTTCCTATGTACAACATGAGAAAGCATTCATGCACTATACCTTTTATTTTTTGTTTGCTGCTGCT
ACCTTCTCAACTTTGCCGATCATCTGAATAGCAGTGGCCTGATTTTCAGAGGCAGTGAGAACTGAGGTGA
ATGGGATCTGCAAGTATACAGTAACTCAGAAAAATACAGCAGCCACTAGGAAAGTAAAACAGGCTGCCCA
ACGAAATTCTAGAGTAAGGAGAAAAGTGGGCTTACATTAAGAATCAGGATATAGACCCTGAAGTCAGTGG
TCCATTTGTTTGGTTTAAGGTACTTCCACATTCCTCTCGCAGCTAAGAGAGGCTTTGAAGTGAGCACCAC
CTATCTTTGTGCATTACTTACAACTCTGAGAGTAAAGAAATCTGGTAGCTGCTTGGCCATTCTTTGACTG
ATAATGAATGAATGATAAACCTAAGAATTTATCAACAGGTTTCATATGGTTCAAAACCAGGTGTGTATGA
ACTGTGTACTTAGAAGGCCAGATGAACAGAAGGAACTTTCTTGCCAGTGCCATGTGAGTTATCCTTCACT
AAACTGGTTTTTTGCACCGCTCTGTGAAAGACTGAAGAACTGTCACTGACAGCTTTAGTCTCTGCAGCTC
TGACTTGAATACTGCATTTTACAAAACTACAATTGCTAAGAAGAACCTAATAGAGGCAGGTACTGACTGA
ATTATCACTGAATGCGAATGAGAACTTTGAGCACAGTGGAAATTCCAGCCTACAGATATAATGTTCAATG
CTGCTGCTTTAGGTAAAGAGGAAAGCTACTTTCTAGAGATAACAGAAAAGTGAAACTAAATAGAGAGAAA
AACATATCTTGTTTTAAAAAACAAACAAACAAATATTGGGAACTAAACATATAACTAAGAGAAGCTCTGG
GCCCAGTTCTCCAAAGACACTTACTGTCCTCTGCTCCTAGAAACTGAAGACGTACAGTTCTTACCAGAGA
CAAGCTCCAAAATTGTTTTGGAAGTGCGGAGAAAAATTGCAGATTGCAGCAGACACTTACGTGGAATAGG
GCTCTGGTGGGACTGCAGAATGACAATTTGCAAAAGGACCATCCAATGCAGTCAGAATTTGACACTTAGA
AGTGCTGTTTTCTTGCATCAGCTTATTTTCACTGCAGCCTTGATAGAGCCCTGTATCTGGTTCCTCATCA
GGCTCATCTTCCACAATGGTTTCTCGCCTGGAAATGAAATCATTATGAAACCACCAGATCTCTGTACATC
CAGCACTCATAGCACTAATTTGCAAGTAACAGCATTTCTGCCTTTTGATAGAAGTGTCAGCTTATTTCTC
TCTCAATATTTATACTGAATGTGAAATGAGTATATAAAGCAAATCAAAGATGTACTAGAATAATTCCTAC
CATTTCAGAGATATATGAGTTTTTAAATAAAAAACAATAGATGTTGTTATCTCCATGGACATTCTATATG
ACATAGGTTTCATCCAGGCCTCCTTCACCTAATCCATTCTATAATAATTGAATGCTAGGCAGCTGGAGTA
GACCACCATTTATTGGAAGTATGTTCACTTTCACTGAAGAGCATGCCTGAAGTGTCAGAATTCTTCTGCA
GGTATAGATTCCAGAGCCTCCCTATGCTCAACATTAGCCTACATGCTGCTTTCTACTATCCTGCTGGACA
GGTTTTTGCAGCCAGATTATATCCTGAAATGAATACAATTCTATTCATGTACTGGAAAAAAACAAATAAT
ACAGTAATTTCTGTTGGTGGTTAAGCTACTCAAAAACCTTTCAAAGCAAATTGAAAAGGTGGAAGGAGAG
AAGAAAACTTAGTTCCTTGCTTCAGTGTTCTGTATGGACTAAATAGGGCAGTTTGGCCTGATCACAAATA
TCACACTGATTTTTATTCAAAAGAATGTCCCAGGTTTTTTTTTTTTTTTTTTTTTTTGTACTAGCAAAAA
ACACAATGTAGTCATGAAAGTAAGGAAGTTAGACACTTGAAAAATCTCTTCTGTGAGGAACTGTGAATTC
ATCCTGATGGATTTTGAAGGTGCCTTCATGTTCCAAAGAGAAGGATGACACTGAATCAGGATCTGCCTGT
CCATCCAGCAAATGATGCATTTAATCTTTGCCAGGACCCATGGGCTTGGCAGATACCTTATTTATCCCTG
TGCTCTAGCTGTTGTAATGGCTGTGACTCCAAAGGAAGCTGAACTAGAGCAATGCACTGAGAGCTCAGTC
CTGTTTTGGCAAGATGGAAAAGCACTGATATTTCACCTTAGTGTTGAGCAGGAAAAGTCCCCACATGCAG
ACTAAGGGAAGAAGTAACAGAAACCTGACTGCATAGTTAGACCTGAATATTAACTATGGCTTAATTTCCT
CACTTCCAGTTGCTGTGTTTGCTAGGGATATTAAGTAGAAAATTAGGTACCTGAATCGAGAGGTTCTTCT
TTTCACAGGAACCTTCCAGCTCTCACCAAATGCATTCACATTCTTCAGCCAAACACCATCTGGGTTTGTG
AAGTCATTTCTAGGTCTCCCGTCAAAGTCACCACACAAACCTTCTACTCTGTTGTTGTAGGTGTTGGCCA
GCTTTATATCTAGGAAAGTTCAAAGGAAAAAAAATAGCCATTACAAAGAAATATTTTCTTCCGAGTATAT
GGAGGAGGTGGAAAGAAGTGTGAAGTACTGAATTCCACTATTCAGACTACAGTTGGTAGTGTGATGAAGG
ATTTGAAATCATCAGAAATACCATGGCTGCATTACATTGTAAATCAGGGGTAGCTCCACTTTGGATAGAG
GAGGTAATGTGTTTTCAGCTTACATGAATGAGTTCTGTATGCATGATATGAAAGTCCTGATGAGAAACAC
TAAAGAATGGGATTATCTGGAACACGCTTGAGAGGAAGCATGTAAAAGAATGCACTCTCAGTTGTTCAGC
TTGAGGACCTCAGTCCTGTAACTGGGTTAAGCTGGGAAAGGGGCAGATCCAGACTTTTCCCCATCGTCCT
GATCAGCAGCTTAAAGTAATTTGCTTTTGTTTCCATCACTCAGGCCTTACCTAACATTAAAAGTAGTAGA
ACAGTCTGACTCCACATACAATTTCTCCTAAATTCAGATGGATCCAGATAGAGAAATGCCAACTTCCATG
AGAAATAATTTTTGTTTCAGTTTTCTGGAAGTTCATGTCACAAAGAACCACAGACTTAAAGGGTGAAAAT
TTCTAATTTTATCTTGGGTTTAGACCTACTTTGTAATGATAACATTTTTTGACATGGTGCATAAATATTC
CTGCTGATTGTTTTAGTATCACTTACCTGCATTTTGATCACCATCGTAGCTCAGGTATAAGCCAAAATCT
GTCTCCAGGTAAATTCTTGTTGTCCTCTGATATATACGAATACCATCATGGGGACGAACAGGGGCTCTCA
CCCTTACACCATCCAACTAAAAGGGAAAGAATAAGTTAATACTTATAATACAAATTCGTATGTGGCTTTG
ATGCAGGTAAATGCTACAAAAGAGCATTCCTCTTTTCTGGTTTTATGATAAATATATAAGATATTTTAAC
CAGGTAAAAAATACAGTATTCCACTTTTAAAGTAACAAAATCATATATTAAGGAAAGTGATCTGGACACA
GTGTTCTTACATCATTATCATTCCTGAGAGATTTCCAGTACTGAACAAAAATGCATGTATGAGCCCAAGT
ATGCTATTATGGACCAACAAAGAGGACAGAAACTGAGTTTTGCCAGCATAATTCACACTTGTCCCTACCA
CTGATCATATTTTATGTGGCTTTTTGGTAAGGTTTGGATATTTGGTATTTTATTCCTAGAAGTCCATTTA
TTTATTCATTCTAACTAGAATCTATCTTTCACAGCTTTAGAAAAAAAGAACACAACTGAAATCACACTTT
TGCTTGCCCCACAGTACTGCTGTAATAACACTGTTAGCTTTAATACTCTTTAGAACAATCCTTTAATTTA
TCTGTTTTACTACAAAGGAGAACCTTTGTCTGTGCTCTGCAGTTAGTGAGAGACTTACCAGTATCTGCTT
GCCTTGCCTGAATCGCACTTCGTGATTGTAAACGTTGATGATAATCTCTTTCAAGTAAGTAATGCGTCGG
TTTAGACGAAGAGGATAGTTCACGCCTTCGACGCTGAACTGTGTCAGAGTATCAGGTAGGTCAGGGATGG
TCTGAGTCAGAATATACGTGTATTTCCCTTGGAAGTGGTGCACCAAACCATCAAATGTGATATAATGTGG
ATCGCCTATGATCCGGCACTTGCCAAAACCTAAAGAAAAAGAGTTTAATTGCCTTCCAACCTGAATGCAC
GTACCAAATCTTATGCATAAATCTGCTTAATACAGTTTAGATATAAATAATTGCTGCTAACATGCAATCC
TCTCAAAACAAAGACGGGTGCTCAGGCATCACCCTAGCAAATGGGTACTCAGACAGAAAGCCAACCAGAG
AAACTTATTCTTCTGCTGAGAACCAGTGGGAACCAATAGAACTGAAGCACGGCATTAGGGTTTATTGCTT
TTATAGGCACTAAGCTGCACACATCAGTCCTCTGCAGAGGAATGACTGCCACACAGGACGGCTTGCAGTG
GGCTTGTAAAGATGTTGGTTTCATAATCCTCAGCAATATGATCATCTGATCATCAATACAGTAGGACCTA
TGCCATCCTATTCTTGAAAATTCATGTGTAGAGGTGTTAGGCAACAAGCTACCAATTAAACTGTCCAATT
TACAATGAGAACATGGGATTAACCATAACATAGCAGGGCTGACACCTCAATGTAAAAGCTTAGCCAAAGC
AAGTACTGGATGAAAAGCACCAAGATTGGCTATTAAGTATCTGTTGTGATCTGCTCTGTCCTGTTTTGCC
TTTATTTTTCATATTTTCAAATCAATATGCAGTGAAGAAAATAAAAAAGAATGCCAAAGGATTAATAGAT
GTATATATTAGACTTTTATTTTTAGTAAATGCAGTGGATATATATATATATAATCTGCCAAGTTTCTAGA
TGTACATAGATTTTCAAACTCTTGTTCTAATTCTCTCTACAAATTCCCTGTCTTGTACTGTAACTTTCTA
TTCTGATAAATGAGGCCTTTTTTTGTACTTAATAATGTTTCATCAGTAGTCCTGTCAGACCTGTCTTTTC
ATGTCGTAAGGGTTGGAAGTGACATGGAATAAAGAATGGACATTCACATCAATCCTCCTTCTCTAGTCTT
CATGGAATAGCAGATGAAGATCTGCCTGCACATACCTGTTGGATTGCACTTGTATTTTCCACTTTTGATC
AAGCAGGTTTCTTTAGAATCACATCCGTAACTCTGACATCTGATGACACCATTCTGCTGACATTGACATC
TTTCGGTGCAGTGTGGAGTAATCCAGGTCTCCCCAGCCTGGACACAAATTGTGGTATGTTACCAAGGTGC
TGAGCAATAGAAAGTCAGTAGCAAAACGAGTGGAGTCTGGCTAGGTGCATCCTGCTGGCCATTCCACAAC
TGCTCAATCCAGGTGTAAACTTATCAAGAAGCTGGGGGGGGGGGGGGGCAATCTCAGTCCATTTATCTTC
CACAAGGGAATTGGAACACATAACAGGAATAGGATATACCACCCAATTAACGCGGTCAACTTTCCAGTAT
GTCTCAATTTTGGCTTCAAGGTGCAAACGACCACATTCGTGCATCCTCTACCAACTTAAGCCCATAGCTT
TTGCTAAGCAAATGAAATGTATTTAAAATGGTAGACCCTGAAGACTTTTCTTTCTCATTCTGTTGGACAC
AGAACCAATTTCCTGCAAGTTTTGCAGTCAGAAATTTACCCTACAATGGACTACTTACACTGTAGTAATT
GTTGTCATCATCCCTGCAGCCACAGTTGCTGAGAGGAACACACTTGTCATTACTGAGCACATAATTATCA
TCACACTCACAGCCCTCTGTGCATGATTCTGTCTTCTCACAAAGGGATGAGGCAAAAATGTCATTGCATG
TTGATGGGCAGGCAGAGGCACAGGCTGTGTAATGGCTGTGGGATGGACAGGGCAATGCTGTGTGGGTGAA
GAGAATAGAAGTAGTGTTATTAAACACGTTAAAACAAATGTTCTTGCCTGATATCTTCAACTTGGCCTGT
AAAATAGTGGTGTAGTTCTGCCTTCTTATAGATCATTTTGATCATCAAACAAAAACTTTTTCTTCTTTTC
TTTCTTTTTTTCATAATTAGATGGTAGCTTTGTTGAATGGATGGAGTGTGGCACATACAATGGAAATTAA
TCCAAGAACTTGGCCCCTAATAAGCAACTGTCTGAATTGTCAGCAAGTGGCTTTGATCCCTGTGTTCAGT
TCAGTTTCCCATTTGGTTTTTTACTTCTGGTTATACTACGGTTATTATAGTTGGACTTGATGATCTTGAA
GATCTATTCCAACATAAATGATGCTGTGATTCTATTATTTCTCCATTTTAGACTCACTGCAGTATCTCAA
GACATTCATTTGGCTACCAAAACCATTCTAGCTCCACACTATCACCCACAACTCATCTAGTTGTTATCAC
TGGGTTTAGTTCAGCTTGCAAACAGTTCAGCAACATAGTCAAGTCATAAAATCATAACAACAGAATTGTT
TATTTTGAATATTTTGTCTTAATTATCTTTTATATAACTTGTCTACCTAATACTGCTCATCTGTCTTATG
CTGTCAGCCATCCAATTTTCTGTTTTTTTTCACTTTCTTTTGCACTTACGACAAAATGTGCTATTCCTCC
ACGTGATGGTGATGCCTTGATTCCTGCAGGTGTCCACATAGAACTGAACTATGTCACAGAGGGCAGACTT
CATGCCATCATATCGACACATGTCATAGGTGCAGATCTTTATGAAGTCTTCGGGATTCACCAGATCATGA
CATGCTTTGAATTTGTCTGATTTTAGGACGTTGCACTGACTTTCAATGATTGGTTTATTCTCATCAGTGC
AAGGAGGAACATCATCTTCTCTTGAGTCTGACAAACAACTGCAAGAAAGCACAGAAACAGCTGCACTTGA
GTTTCAAAGGCAAATGTGAGACTGAGACAGAAACTGGAATGTTTGTTGCCACTGAAAGGTACTAAATAAA
TAGTGCTGGAGACTGCAGTCTTACTTCTGTCTATGGCTGGAAGAGTAGCAGCAGTAGATGTGCATGACAA
TCACTATGCTGTTTCTTGACCTTGGTTATGCTCACTGAACCTGACCCTGACAAGAGGACTGATTTCCCAT
CTTGACCTTGGACCTGCCTCATCTCTGCGAAGTTGTGTGGTGACCTGGACTCTTTGCTGAGTCTGGCTGC
CACCCTCGGGCACACACACTGCTTGCCTTCTGTGGACACTCTGCATGGGCTTTGGCTGGTGAACCCATGG
TTGTACAAGCTGTGTTACCATGCTAGGCTCTCTGTACCTTAGGGAACAGCTGGCCATCACTGTTCCTGAA
CTCCATTCTCCTGTTCTAAGTATGTTTTTCAGTGTAAAATAAATGTAAGCTGTGTCCACACTGTGGACTA
CTCCAAAGCCTAATTCCCCACGTGTGTGTGTCCACAGTAAAATGGCTATGGTTTCCATCTGGCCTGTATG
GTGATTTCTGGTGGTGATGGCTTTTTTTCTTTTGAGCTTTTAACTGAAGAAGACTAAAATCTCATACATA
CTGTACATTACAAAGTCATAAAGTAGACAAGGCAATTAGAGGAAGACCGTTCCAAAGAAAGATGGAAGGT
ATGAAGTAATGCTTACCCTGCATCACTGTCGTCTTCTACTTTCCAGCTATTTCCAAACTGTGTGACACTG
ACAAGTGTACCATTGGGCAAGGACAGATCATCTTTATAATTGTCGTTGAAGTTACCACACATGCCTTGTA
CCTGCAAGCAAACAAGAACTATACTCTAAAAACAATCTGCTCTGCACCAGCTTCCCCAGTAAGTTCTTAA
TCATTGCCTCTGGAATGATAAGGTTGAAACACTAATGACAATCAATCTTATACTAAATATTTCAGACATA
CTGATTGCACAGTAGAAAATAAATAGACACTATGATACCCTAAGACTTCAAACCATGTAATTCTATAAGC
TGGATGTATTCCCCTAGCTGTGTCATTTTCTGTCAGTTCTCATTTGGAACACAAGGAAATTGGTGCGAAG
AGAAGAAATAGGGAAAAAATGACTTGTGTTACAATACAGCTGTGAAGAAAGATGCAATAGCAAAATACAA
GAACAAAGAAAATGAAAATAAATACACAAGCAGTGGTTTTGAAAGAATACCAATATGTCTACAAATTAAA
TCTTACCTTTGAGAAGTAAGATTGTGGGAGAGTTATTTCCAGGTGGTCATCGCCATCATATTTGACTATC
ACACCAAAGTCTGTTTCCACTACTAGGTAATTGCCAATATTTCCAATGGACACCCCCCGCACCCGGTTCT
CCACTGGAGTGTAGACCCTCTCACTGTTCAGCTGGAAACAAAAATCATTTGTCAGTATGGATGATCTCCC
ATGAAAGCTTTTCTGCTTGAATCTACATTCCTTGGAGTGCTGAAGATTACTCTGACAGTTTTAGATTCAT
CACACTTTCACTATTACATATTCCACCTACAGGTTGCAAAAATCAGCTTTTCAGCTGAGGTGAGCTGGGC
AGGGCTCTGGTGATTATAGCTATAGGTGACTGGCCTGACAACAGTTACTGAAGTGGGGAGGTACATTTTT
TAGTTCAGAAAACAGTTCTCAGATCTGGAGGCTCTGGGCTCCACCTCCAAGAAATAGCAAATAGAACAAT
GAAACGAGAAGACAAGCCTTGGGAAATAGTCAGGAATTATATTTCCCCACAACATCAACTTAAGGGAGGT
GCCTGTAATTATTAAGGATATGCATACACGTTTCTTATGAAACATAGATGTCCTTTTTCCTACAGCTACT
GTAGTGAGCTAATTTTAAGATAATTCTTGCTGGAATAAATAGCGCCAACACCTACCAGGATTTCTTTGCT
TTTCCGAAGGGTGATTCGTGTACCATACACATCAATGTAGACTTCTTTCAGGTATGTGGCCCCTCTTAGC
CCTCGGTCTTCGTTCTTGCCAAGGATGGTTATTGGGTTGACATTGTAGCTGACAGACTTAACCAAAGTGT
AGGTGCAGGTACCCACAAATGTGTACATCAATCTATCAAAGGTGAAATAGTGGGGATCTCCTGCTACCTG
ACACGTTACTTGGCCTGAAAGTGAAAAAAAGAAAGATCTAAGGACTTTGGCCTGGATTTTACCATGGACT
CAACTGACCATAGACATTTGTATTAGTGCAGCAACTTAATCCTCATCACTATTACAAGGAGAAACTTTCA
TAGAAGTATTTCAGGTTCATGTCAATGGCATTTACCTGGTAATGAGAAATTTCCAGTAGGTATCTCCTAA
ATTTAATTAAAAATTACATACAAACTGGCAACTTGGCAACAAATTAACCTGTTTATCTGCTTCTATATGC
TGCTGTATCCATAATGCATGCTAATTAAGAGTAATCCAGAGCTTTCCTATGTAGCAATTTGAGAATACCT
AAATATATGAGTAAATATAGGTATCAATATGAATGTAAATAAATATGTATCTATATACATAATTATGTAA
GAAACTTGCCAACAAACACTCTCTCAGAAATGGCTCTCAGTCCCAAATTTAGAACTTGCAGCACTGGTGA
TTTGTTTCTTGAATTTGTATGATCAAGACAAAAAGAATCCAAGGGACCTTACAGGCTCTTAGGCAGCATC
AATTTTTGCAGATATCAATTACAGAAGAAGGGAATCAGTGACCACAAGATAGATCATCATGTCTTCTTGT
ATCTTACCACTGGCAGCAGTTAGTTGGAATTATCATTGCCATCACATATGAGAATGTATTTATGAGAAAG
TTAGAGAGAGAATAAGAATCTAATCATAGAATTGACACTGAGAAGAATTTTCTGGGAGGACTTACCACTG
GAATGACAGCCCAGCACACCATCCTTAACACTGCATTCCTCTCGTACTCCACACTCCCAGGATGAACAAG
CAATCATGTTATTAGCATTGCAAGTGCAGCGTTCAGTGCAGGAATAATTGGTGAACCAGCTTTCACCCAG
CTGAGGGGAAAAATATGTAGAATTATATACTATAAGATAAATAGGATAGTAATGCCATAAGGGACAATCA
AATTACTAGTATTTGAATGTGCACAATATGTGATCATTTACTCACTTATGACAACAGAACTCAGTGATCA
AGAAGGCTGGAAAAGTGAATCTTTTTTGCTATTACTAACAGTGTAAATAGCAATTGTGATAGTGTTTTTT
CTCACAATGATAGCAATTAAGACATGTTGTTCGTTCTTTTTTTTCTTTTTCTTTAAATATTGTCTATGGT
AACTTGCCTCATGATAGTGGTTTTTGTCATCAACACAACCACAGTTGCTTTCTGGCACACATGTGTCTCC
ACTCAGAACATAGCCATCCTTACAGAAGCAACCCTCCACTGGCAATGAACTACAGGTACTAGCAGCCGAG
GAGTTCACACAAGTAGAGGGACACCTAGTACCACAGCCTTTGTAGATGCTGCCTCCAGGACAGGATATTG
CTGGAGGAAAAAGGAAAAAATAAGATCACTAAAAATGACAACATTTTTATAAAAATTATACCGATGCTTT
CATTTCTACTGTTCGCTTCTGAAAATTAGGAAAACTGTTGTTGGAGAGTCTGACACTGGAGACTACAGAT
TACCAAGAGTCTTCTTGAACAGTGAGATGGTGGGTGGCATCTGGCAGTGGTGGGCAAGACCAGTAGGCCA
CCCTGCACCTATCTCACATAGGAAGTCAAACATTGCAGTCTTGCCTTGAGTGTAGGGCTGACATGATCAT
ACAGACCTAAATGTGCTTCATTCTGTAATCCTTCTGGAGGTTTAAAGACATCCAGATGTGCTGGAAACTC
TAATCCTTCTGGTTTTGGCTGCCTTTAGTATCTAAACAGGATTACAGCAGCAGTGCAGAGCTGTCTGGTA
GTTTTTCTGAAAACAGCTTAGCTATGTTCAAAGCCCTGAAGATCACTAGTAGGTGACAGAGGGCAGTAGG
GAGGCCATGGAAAAGAGGAGCATGGAAAAGATAAAACCCCTAAGTATGTACAATGCAGCTATGTCATGAC
AGATAAAAGTTGCTGAGGCACTGCTTTCAAGTTTCATCAGAGTCAGAACCATGTATGTTCTGTCTTCCCA
CCTTTGGGCAATTTAAGACAGAAAAGAGAGAATGGGGAGGAGCTGGTTAGAAAGCTGGGGCTGGCCAGGG
AAGATATAAGGCTGCAGGAGGGATTACATCTTTACAAAGCCAGTTTGCATTACATGGATCTAAACTTCAT
TTTCAGCAGTATAGTGGACAATAGTAAGTTTCATAAAGGTTTAGTTTGAACCACAACTCTCAAGGCTACT
CATATCCAGGGATTAAATAAAGCATTTCACTCCTCTGAGCTCTGTTATTTCCTGACTACTCAAGTCACTC
AATTAAGGGAGTAGAAGCATCTCTTCACATATCAGATGGTATGTCTCTGTTACTGCAGTTACTATCTGAA
CACCGGTCACATTTAAAAATGGACCTAAAAATCAGATTCTTTTCCATTGAGACCCCCTGAGAAAATACAT
TGTGCTCTCACACCCCTCAGGGAAAATACTGTATTTTACAAAAGTCTTGAATCATTTGTCTATTCCTGTG
GTTTGAAAAAAACACCAAATAGTATTTTTGTGACGATCCGTGAAAAATCACCACATGTGAGTCTCATTCA
CTTCCATAACTACAGCAGGATCTCTTACTCCTGTCAGACAATTACAGCCAACACCACCAGTCATCTATCC
TTTTCAGATTCCTAATTGATTTTTTATTTGAACAACTGATGGAAGGAGCATTTGAGAGACAGTCCTACTA
ATGTGACTGAGCAATCATCTTCTCTAACACCATAACTAAGCTGTAGGTATTTAGGTCCTGGAATATGGCC
TTAGACTGTAGAGCCTGGAAGTCCGTACTTTTAATCTGAGTTGGGGGCCTCAAAGCTATTTTCTTCTTCT
GTCTTCTCTTTACAGACAAAAGGCCAGTGCTCTGCCCTAGGGCTGACTTATGTGTGCTTTTAATCAAAGG
CCATTCTAAAGTCATTCTTTTACCAAGCAGCTCCTGTCATAAAATTATTCCAAACATAATTAGTATGGAG
ATAAATGAGAATACTTCTGTAAACTTCCTAGAAACACTTGTTTCTTAAAATGTCTTACTAAACTAATTTT
TCTCCAAAGGCCATCAATGAATATTGAGAGCCTACAAATATCAGCTATGTTCGTATTGCGAATGGTGGAT
TTTCAGCAAAGCAACGTAATAGTTACTCTCCCAGTGCAGAAGCTCACAAGAGATACAACTGACTATAATC
CAAACTTTCCAATAAAATAATAGGAAATTATAAATATTTGAAGAATTAGACTAAAATGTATCCAAAAGTT
CATGAAAATTATTGAATTATGACTCCCGACATACCAAAAGACTAAACTCGAAGCACTACATATATTGAAA
CATCATACATGAAAAAGATAGAGGAGAATGTGAATAATTTAAAGTTTAAATACAGAATAAATATTTATAA
ACCACAGCACTCACGGCAAAGAGTAGCATTTCTCCACTCTATACATATTCCAGCATTGATACATGACTCG
GCGTAGGCCTGCAGTCCATAGCATAAGGATGTTGCCTGTCCCTCAGTGAAACACATGTCATAAACACAAT
TATTGAAGAAATTTTCGGGAGGCACTTTGTTGTGGCAATCCTTGAAGATTCCTGTGTAAACAAAGAAAGA
TACATAGAGGACAGAAAAGAAATGGGGAATTTATCACCATCAGCATCTATTGTCTGTCATGGCAAATGTA
TTGAGTGCTCCAGGCTGACTTTGTTTAGTAAGTTAAATCAACACTTGAAAATGAGCACATACAATAAAAC
CTGGCCCTCTGGATCAGGCTTGTTTCTTCTCCCAGTGTGATTTACACAACACAGCATACATAAATTAGGA
CCTGCTGGCTAGGCAAACCAATAATTACAAGCAACTTCAGGGAATCTCTAATGCAGGAAACATCTACAGA
ATTTCAGATGAAGTATCTATAATTTATGCAATGACAGTGCCCTCTGAACTATTTCATTTAGAAAGCAAAA
TGCAAGTCACTACAAATCATAGAGCAGCTATGGTTTCTGGTGATCATTTTCACATAATCTACATGAACAT
CTTGGCACTGTCTCATCAGTTGACATATCTTGACCTATGAATTTAATTTGGCCGTTGGCACTGGATATTT
TATACACATTCAGTGTAAGGTCAGAACCATGGGTAAAAAGACAGCTACAAGCATTCTACCTGTTGGGTCT
GTGATCATGCCACATGCTGTATTCTTCTTTGCTTCACTCTCCAGCACAGCATCACATTGGTCTTCTGTTC
CGACAGAGCACCTTGGGAAAGCAAAAAAATGAAGTGTTTATATACATCTCTTATTACCAGATCTTTTGTG
GAATTCTGACGCACAATAACCCCACATAACAGTGCATTTTGTTACAGTCAGAAAAATTTATCTAGCTTTT
TTGAAAGGATGATGTGAGCCCATTTCTGAATGAATGCCCTTTTCAATGCCTTTTGGGCCAATCAAAAGAC
TGTTCTTGCTGAAAAAAAAAAAAGAGCTATGGGATCCTGTAAGTTAATGATTTCCAGTCAGGGATCTGGA
CAGCAATCACTCCTGACTCCCATAAACAGTTCTTGCTTTGGAAAACAAGGCTTAAAGGATGCAACCCCTC
TTCTCATTCCCTCTTTAATAAGAAGAGGGAGCTCCGTACTTCTAGTGGGTAATTGGAAGATGCAGAGTAG
TACCTGGATGACAAAGCCAGTGAATGGATAGATATTTGATTTCATAGAGCTTGATTAGCAAAAGTGATTA
GATTCCTGTACATACATGGTGTTATTCTCAGGTACAAGCCAGCTTTGTCCAAGTTCATTGGAATTACTTG
CAGTGTCACCGTTGGGCTTTATGTTGTCATCATTTGGATCACCATTATAATTACCTGTAAGGGTAAAAAA
AAAAAAAAAAAGGATTTATAGGATACATGAAGTTCACACCTGTAGAACAAACATAGATTTCAGGCTATAA
AGGAAAATGCAATGAACTAGTGAGAGTTTATATCCAATGCCAATGTGCACCGGGCAAAATAAGAAGAGTA
CTTGTATTTGGATCTCATCTGTAGTGTTGGACTAAAAATCAAATATTATTGTCATTTGCATCATTACATC
CTTGGGTAGGTTTCCTGAATTGAAAGTGAGAAACAACCACACAGAGGTAATTTAACTAGAATATTTCAGA
TGATCAAGATCAAAGAATAATCAGAAAATCAGAAACTCATAATACAAGCTACTCTTACAATTTCTACAAT
CCCATTTCCATCCTGAAGTATGTTTCATGTAATCAACTGGATTACAGAATGTTGTTCCAACTAAACCTGC
TCAGATTCAGGCATTGCCAACGTGACTGATGAAAAATGACTGATTGCTACTAGCACTTCACTCTCATTAG
CTACAAAAACCATCTGTGACTCTCAGTTACTTTGTGTTCTTACTGTAAAAGAACTTCTTACTGTAAAAGA
ATGTGGTAGAATGTGCCCTTTGTGTCTGACCTGTTCTTTCTCAAAGGACTCTCAAAGGGTTCTATCTTTA
GTGTTGTATGTTAATGTTTTGTGGCATTTGAATTTTTGAAACTACATTTTTTTTTAATCTTATTCATCAA
GTCTATCACCTGAGGATCATCCATACTTGATTCCTTCATTGCTTTGTTTTACTCTAGACATTACCTATCT
AAGGTAGAATAGAATCATTACTCACTCTAATTTACACTGATAAGTGAGTTTTGTGCTTTTTTTTTTTTTT
AATCCAGCTAAAAATATTATTTGTAGCTATACTTCAAAGCATTCCAGACAGATAACATGTCACTTGATGC
TTTGTAACAGTGTGCAGAGACTGAAAAAAATCAGTATTTTTTCTTTGCTGCTGTAAATTCTAGTTCCTTT
GGTAAATGGTGGTTTTATGTAGATTAAGTATGAAACTAAGAAAAAGGTTTAAGTAACCACTAGTCTATAC
AACTCACTAAAAAGTGATTAGTTCTGTGACTTACCACACAGGCCACAGAGCAGGCCACTGTAATCAGATG
GCACAGAGACTTCTGCATAGTGATTTCCATCATATCTCACCCACAGTCCAAAGTCAGTTTCCAAGAGAAC
ATAACTACCACTGTTTTGAATACTGATTTTCTTTTCAATAAAGATGGGTAGGTTCATTCTGCTGCCATTC
ACCTACAAACCAAGTTGTAAAAGTTAGTGTATAGCTGCTGAAACTAATCCAACTACAAGCAACTGGAATA
GTTTAGTGAAAGATGTCATTTTGTGCACAAAGAGAATGGGACACAATTAGATAATATTTCACCTTAGCAA
ATTTTTTTGAAATCTCATTAAAAGAGGTACATGTATCCAATGACAAGAGAAAAATTACTATAAATTCTAC
AACAAACAAACAAACAACAAAAAAAATTAATTATCTGCATGTTAGTTTAAAGATGTATTTCAAACAAGTA
TTTTCTTACATTCACTTTCTTGCTTTTCAGCATAGAAATCTGGTAGCCATAGACTTCCACTTGCACTGAC
TTCACATAAGAGACTTTGGTGTTGGATCCTCTGTGCTCATTTGTTGTGGACACATCAAAGTATGGAATAT
TCTGAGAGATGTTACACACTTTGGAGAGGGTGTAAGAACAGTTTCCCATGAAATTATGAACTCTGTGATC
AAAAGTGTTGTAATGTGGATCCCCAGAGGCACTGCAGGAAGCATGGCCTGGGAAGAAAGACAGAATAGTT
TCCTCTGAATCTCTGCTGTAAATGCTGCAGTCACTTTGTTTCACTTCCTAATTGCTGTTCAGTTGTGATA
GTGATGGCTTCAGTCAAGAACAGAGTGCAAAGGAGGTATGCAAACTTTTCACAGGAGAGAACACCCTTCT
ACCATAACTGGAAGTCACCCACATTCTGTGACAATTCATGAAGAGCAAGCCATCTGATCCAGAGACTCAT
TTTCTGTATAGTAGAGACACACCATATGAAACATGATACCTAGAAGTTGATCTGAATCATGAACATCCAA
ATAAATAGGATTTTCATGCCAATAACTACCTTGAGATTGAAAACAAGACATTCCAAAACCTGGGCTAAAT
TCCAGTCTTTGTACCCCAAAAGAGTATGTACTCTGGTGTACCAGAAATCTCTGCTAGAGGCCAATGAAGA
AATATGAGAAAAAGGATATTAAGACATCAGTATATAAACTGGAACAGTGAATTTGCAGTGGCCAATTCTG
AAAGGCTATCTCTGAATATATAGAAATAATCTACGCAAAAACATTACAAGACTGGCTCCTCTGTTTTGGC
AGCTGGAATGAACTGCTGCAATACCAGCTGTCTAGAGTTCTGTAGTTATATCAATTATAGAAATTAAGGA
AAACCTCTGACACTCCAGCATAACTCTGTTAATTCGGCTGTTAGACCTGAAGTTAGAAAACTGAACAAGT
AAAATAGGCAAAAAAGAAAAAGGAAATAGGAAACAAAAACACATGAGCTTAGCTGGGGTGTGGATGGCAT
TTGGGGAAGCTTAGAAGAGCTGTCAGGATATGGGCTTGTAGGCTTTGGGGTTGATGGAACTGCAATAAAT
CAGAGTTAAAGGAGGTCTGATTCATCATGAGACATTTGCTCCAAGTGCCTGACACAAATTGCATCAAAAT
TCTCTATAATGGCAAAAATTACCACAGATAAATAGAGATAGTGAAGTGAAAAACTCACTGGAACTTGGTG
TAGGTGATGGAGTTGGTAAGTGTGAAGGTGGTGTTGAGATGTCTGGTAAGGGTGTCCCAGGTTTTGGAGT
TGATGGACCTATTGTAGGAGAGGTATAGCAGAAATTAGAATCAATATGCAGTTTTACCCCCTGAAGATGG
TACACCCTTTCCAAACACCTGAGTGCCATGACAGTATTAACCCAGGACAAAATATACACAGTAACTATTC
TAAAAAAACTGTGCCAATCAAGTACTCCCCGAGCAGTATGGCTGGGGGAAAAGCAAAGTATAGACCCTTA
AAATGGCAATTCCAAACCCTAGCCAGGGACCTTGAAGGAATCGAACAAAAGAGATGCCAGAAAATGTACA
AAATGGGCTCAGGTCATGACAATACCTGAGCTTGGTGTGGTTGTTATTGGGTGTGGGGGCGGCGTTGAGA
TGTCTGGCAAGGGTGTCCCAGGTTTTGGTGTTGATGGTCCTATTGCAAGAGAGAGGTAGTGGAGCTTAGC
ATCAACATACTGTTTCATTCCCAGTTGCCAGTATGCCCTTCCCAAACACCCCAGTGCCATGACAGCAGAG
GTACTGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCTAATCAAGTACAATCCCAGCAGCGA
GGCTAGGGGAAAAGGAAACCACAACCCTGTTTCTACAGCCATGCCAACCCCTAGCCATGGACCCTGATGG
AAATCAACAAAAGAGTTGTCAGAAGCACTGCAAAACCGGTTCGCGTCATGACAATACCTGAGCTCGGTGT
GGTTGTTATTGGGTGTGGAGGCGGCGTTGAGATGTCTGGCAAAGGTGTCCCAGGCTTTGGTGTTGACGGT
CCTATCGTAAGCCAGAGTGAGAAGAGATAATATTCAATATGCTGTTTAATTCCAAGCAGCTGCAAATGAC
TTCAAAAATACCACAGTGCCATGACTGCATAGGCAAAGAACAGGGCATACACAGGGACTCTTGTAAGAAA
AACCTGTGCCACTCAAGTACACTCCCAGCAGAGAGGCTGGGGGAAAAGGAAACCACAACCCTGTTTCTAC
AGCCATGCCAACCCCTAGCCATGGATCCTGATGGAAACCAACCAAAGAGTTGTCAGAAGCACTGCAAAAC
AGGCTCGCGTCATGTCAGTACCTGAGCTCGGTGTGGTTGTTAGTGGGTGTGGAGGTGGTGTTGAGATGTC
TGGCAAGGGTGTCCCAGGCTTTGGTGTTGACGGTCCTATTGCAAGCCAGAGTGAGAAGAGATAATATTCA
ATATGCTGTTTAATTCCAAGCAGCTGCTAATGACTTCAAAAATACCACAGTGCCATGATCGCAGAGGTAC
TGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCTAATCAAGCACAATCCCAGCAGAGTGGCT
GGAGAAAAAGGAAACCACAACCCTGTTTTTACAGCCATGCCAACCCCTAGCCATGGATCATGATGGAAAT
CAACCAAAGAGTTGTCAGAAGCACTGCAAAACAGGCTCGTGTCTTGACAATACCTGAGCTCGGTGTGGTT
GTTGTTGGGTGTGGAGGTGGCGTTGAGATGTCTGGCAAGGGTGTCCCAGGCTTTGGTGTTGATGGTCCTA
TTGCAAGAGAGACGTAGTGGAGCTTAGAATCAACATACTGTTTCATTCCCAGCTGCCAGTACGCCCTTCC
CAAACATCCCAGTGCCATGACTGCATAGGCAAAGAACAGGGCATACACAGGGACTCTTGTAAGAAAAACC
TGTGCCACTCAAGTACACTCCCAGCAGCGAGGCTGGGGAAAAAGGAAACCACAACCCTGTTTCTACAGCC
ATACCAACCCCTAGCCATGGACCCTGATGGAAACCAACCAAAGAGTTGTCAGAAGCAGTGCAAAACAGGC
TCGCGTCATGACAATACCTGAGCTCGGTGTGGTTGTTAGTGGGTGTGGAGGTGGTGTCGAGACGTCTGGC
AAGGGTGTCCCAGGTTTTGGTGTTGATGGTCCTATTGCAAGAGAGAGGTAGTGGAGCTTAGCATCAACAT
ACTGTTTCATTCCCAGCTGCCAGTACACCCTTCCCAAATACCCCAGTGCCATGACTGCAGAGGTACTGAA
CAGGGCATAATCAGGGAGTCTTCAAAGAAAATTCTACTAATCTAGTACAATCGAAGCAGAGAGCCTGGGG
AAAAAGGAAACCACAACGCTGTTTCTACAGCCATGCCAACCCCTAGCCATGGACCCTGATGGAAATCAAC
AAAAGAGTTGTCAGAAGCACTGCAAAACAGGCTCGCGTCATGACAATACCTGAGCTCGGTGTGGTTGTTA
GTGGGTGTGGAGGCGGCGTTGAGATGTCTGGCAAGGGTGTCCCAGGCTTTGGTGTTGACGGTCCTATTGC
AAGCCAGAGTGAGAAGAGATAATATTCAATATGCTGTTTAATTCCAAGCAGCTGCAAATGACTTCAAAAA
TACCACAGTGCCATGACTGCAGAGGTACTGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCT
AATCAAGTACAATCCCAGCAGCGATGCTAGGGGAAAAGGAAACCACAACCCTGTTTCTACAGCAATGCCA
ACCCCTAGCCATGGACCCTGATGGAAACCAACCAAAGAGTTGTCAGAAGCACTGCAAAACAGGCTCGTGT
CTTGACAATACCTGAGCTCGGTGTGGTTGTTATTGGGTGTGGAGGTGGCGTTGAGATGTCTGGCAAGGGT
GTCCCAGGTTTTGGTGTTGATGGTCCTATTGCAAGAGAGAGGTAGTGGAGCTTAGCATCAACATACTGTT
TCATTCCCAGCTGCCAGTACACCCTTCCCAAATACCCCAGTGCCATGACTGCAGAGGTACTGAACAGGGC
ATACACAGGGAGTCTTCAAAGAAAATTCTACTAATCTAGTACAATCGAAGCAGAGAGCCTGGGGAAAAAG
GAAACCACAACGCTGTTTCTACAGCCATGCCAACCCCTAGCCATGGACCCTGATGGAAATCAACAAAAGA
GTTGTCAGAAGCACTGCAAAACAGGCTCGCGTCATGACAATACCTGAGCTCGGTGTGGTTGTTAGTGGGT
GTGGAGGCGGCGTTGAGATGTCTGGCAAGGGTGTCCCAGGCTTTGGTGTTGACGGTCCTATTGCAAGCCA
GAGTGAGAAGAGATAATATTCAATATGCTGTTTAATTCCAAGCAGCTGCTAATGACTTCTAATATACCAC
AGTGCCATGACTGCAGAGGTACTGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCTAATCAA
GTACAATCCCAGCAGCGATGCTAGGGGAAAAGGAAACCACAACCCTGTTTCTACAGCCATGCCAACCCCT
AGCCATGGACCCTGATGGAAACCAACCAAAGAGTTGTCAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGTCCGGT
ACTCACCGTTCACCTGGGGCAGACACAACGCAATGCACCGTGGACTCCCTGGGGCCGTCAGCCCCATGGG
GACTGGCTCCCTTCTCACACAGAAACTCCTGCTGCTTGCAGGTAAGACCCTGCCCAAAAAGACCAGCCTT
GCCTTGCAGACCCACCGCGCACACGCCAGACCCACTCCCTTACCAGCACTCGGCTCCTCTCCTGCTTGAG
TATGGTAATGCGCTCTCCATACACATCGACCACGACTTCACGCACGTAGGACACTCGGGGGTTGCCACGA
TGCTCGTTCTTTGCCTCAACGTTGAAGTACGGCACAGAGGTGTTGGAGCACACTTTGGCCAGGGTGTACG
TGCAGTTCCCCATGAAGTTGTGCGTCACCTTGTCAAAGGTGTTGTAGTGAGGGTCCCCGTGGACGTGACA
GATGCCGTAGGAGTGTTCCAGGCACTCGGGTTTCCCATTCTGCACACCACAGAACTGGCCAGCAGGGCAC
GATGCACTGGAGCACTGGACTTTGCCTCCTCGCGTGGGACATGTGCACTTGGACGAGCAGGTGTTGTCCG
TCCAGAACTCTGAGCCCACCGGGTAGTGCTGCCCGTTGTGCCAGCACCCACACTGCTGGATGGGCACACA
CTGACTGTTGTAGAGCAGGTACCCGCTGTCGCATACACATCCCTCCACGCACGGCAAGCTGCAGTTATAT
GGTGCCGTTGGGCTAACACACGTAGCAGGGCAAGCTGCAGCACAGGGCTCGTAGTGGCTGTTGGCTGGGC
ACGTGATGGCTGCCAAGACAAAAGGAAAAGCAGTGAAATCATCAGTCATCCTACACCCGCAGTGCTCCCA
AGGTAGGCCAGATTCACTGGGGGCTTTGGAAGGATGCAATCGCCCCACAGCTCCTCCCAGCTGCCCCAGT
GGGATGCCTTCTACATAGAAAGAACCACACCGGCAATGTGACCAACAAAGGGTGCTCCCACACTCACAGC
AGCTAACCAGCTCAGAAAATGGGGTGCTTTTCTGTTTCTGACTTACGGCAGAATGTTGCGTTCCTCCACA
CAGGGATCTGGACACCAAGCGCCTGGCAGGCATCTGCATAGGACTGCAAGCTGTTGCACAGCGCCTCGCG
ATCAAGCCCCAGCTCACACAGGTCATACAGGCACGTGTCGAAGTAACCGCTGGCACTCAGCACGGCATGG
CACACCTCAAACGGGCCGCTGGTGTCCGTGAGGAGCCCACAGAAGTTGCTGCTGGCAATGACTTGCTTGT
CCGTTTCAGTGCAGGCTGGCGGGGACTGCGTCCCAGCTGAGCAGCTATAGTGAGGCAGAGAGAACAGCCC
CCGGGTTATCTGTGGGCTTCTCCTGGCAGCTCCACTCCACCCCTCCCCAGACCTGCTGGGGCACTACACT
CACCTGCTGTCATTAGACACCTGCCAGCTGTTACCCAGGCTGGTGGAGTCTGGCTCCAGCACCCCGTCAG
GGTTCAGGAAGTCGTCTGCTGCCATCCCGTTGTAGTTGCCACACATGCCACAAACCTTCTGCCCAAAGGT
GCTTGGAAGCGTCACCTCCACCCGATGGTTCCCATCAAACTTCACTCTCAAGCCAAAGTCTGTTGTCACC
ATGGTGTAGAACCCGCTGGACGAGACTTGCACGCCCACAGATGGGGTGCAGGGCAGCACCTCTGGCTCGC
CGTTGACCTGGAAGGACAGAAACCCAGACAGGGTTGGAGCAATCCCTTTGGCACGGCTCTCCCCCGGCAC
AGCCCCGCCACTGCTCCCCTTTGTGCTTACCGTCACCACGCCACCCTTCCCAAGCCTTATGCGCTGGCCG
TACACATCAACGTCCACGTACTGGACGTAGGACACGCGAGTGTTCCCTCCTCGGTGTTCATTGGCAGCTT
CCACGTTGAAGAAGGGCAGTGAGCTGTTGCTCTCGCACAGCTTGGAGAGGGTGTAGGTGCAGTTGCCCAT
AAAGTTGTGGATTTGCTTGTCAAATGTGTGGTAGTGAGGGTCTCCCTCCACCACACACGTTCCTGACAAA
ACAAGCACCAGAGAAACCCTTCAGTTAGGCACCTCCTTCCAGCGCTTCTCCCCTTTCTACAGTGCTAAAA
GCACTGCGTCCCAACGACCGCTCTCCTCCATTCACCTGAGCCTGGCTGTGGTGTTGGTGTGGTTATTTCT
GCCACAGGTGTGGTGGTGCCGGCGCCTGGTGTTGGGGTCACATCGCCTACAAAAGACACAGTGGCACATG
CCAATCAACCCTCCTTGCCCACGCCCTGTTCAAGGCAGAAAATGCACTGACTGCCACTACAAGCAGGCTG
CCTTTCCCCAGCCAGTGTCACGTACCTGGACAGTATCCCTGTTCAATGGACAGATCATCCAGTGCTACGT
CACTGCGGAAATCTTCACCTATCATGCCCTCAAGGAGAATCTAAGAGAAGGGTTTTTTTCCAGCATTATT
TCCAGCCATATACACTGAGTGCTTTTTGAAAGCGCATGGCAGTTAGAGACCCTGTTCCTACCTGCAGGTT
TCCTGCACTGTGCACTGTGACCTCTCCCAAGATCCATCTGTCTCCTTTGCTTCCTGCTTCTTTCCATACC
AGTGTCGATATCTCGTTGTGTAGAACGTACACCCGCAGGGCCATGGTTTCAGCCACTCCGTACATGTGAT
ACCAGAAGCGGAAGCAGTGTGGTCCCGTTGAATTGCAAACTGTGCTGACCAAGATGGCTGTCTCACCTGG
CTTGTGCTCGTTTCCTTGCAAGTAGATATAATACCCATCTGGAAATAACACAGTGACAGTTTCTCATATT
GTTGAACCAGGAGAAGGCTAATTTACTTCATTTACATTTTTTGTGCTAGTACAGTTTTCTGGGAGATATA
ATGACAGTAGTCAGCAAAAAGTATTCATGCTACTGAGACTAGATCACTTATCAACAGAACAGGTCGTGTC
TAGACAGCTCTGGCCAGTACGAGTGGGGTGTGGGAGTATGAACAATTAGCCAGTCTGTAATACTTCAGTA
TACCTCCACCCAGCACTGCCACTAAATTTCTGCTTCCCAAATAGATTTCCAGCAGATTAAATTGTAGTTC
TGCTTTGGGAAAAACTAGCATCTTGAAGTACAGGCAGGCTCTGAAGTGATCCAGTGTCAGCCTGCATATG
GTTTACTGAAGCCTTTCTGCAGCTGAGTCTACAAACATATGTTACAAACATTACATGCCCATAAATGTGC
TGCACTGTTCATCCACCCCAAGACAAATTCCTGGCTGCTGCAGAAAGCTCTGGACAATGCTCAAGAAATA
AGTTGCCTGGATTTCCTGTTAGAAGAGCCTCTTTGTGCTCTTCCCAAAGATTGCTGTATCTGAAATAGAA
CCAGATAGCATTTTCTGTTCCTACCTCCTGTTGTGTGGTCATGGGGAGGGCCGGTATTTGGTGAGAGTGT
TGGTCCCTTGTTCCTTATCCAGTCAAAGTTGTCATTGATCGCCTGTTCCCACTCACAGAAGTCAACATCA
AATGTGCAGGACATATTGCAGGCTGTTGGTGCAGCTATATATGTTGCAGAAAGAAAAGCAGTTTCCACAA
TGAGGTCTGGAATCTTCTCTCTGTTCTGATAATATCCTTTCAGATCTAATGCATTCATGTCCATTAAACA
TTGGAAGATGTTATGGAATGAGTAGAATTAATCCATACAGAAGGATGGATAATAAAATATGAAATTCTAA
GTGTCTGAAAGTGTCTGTTATTTGGCAAGTCCGTTATTCAGTTGTTACCATAATGCTCTAATATCCTCAG
TTTTTACACTGTGCCACAGTCTTAATCTTTGGTCACTCAAAGCTCTCCTACTTGGAACAGTGGTATTCAG
CTGTTCAAAGTGTAGCTTGGGTAGAAATCTAAACACTGTAAATGGGAATGGTCTGACAGTTTTTTTATGG
TTTCTAACCTGCGATGAGAAATGCTGTCTGAGCTAGAAAGAGGTTATGGGATGCCTTTTGATATCATAGA
CTCATCCTGTTCCTTCTGCTGGTTTTGGCAGTCACATCTTGTAGTACAGAAGAAGCAGTTTCATGTCTCC
AACTTTACTACTGTGTTGACTTCTATGTCCACGCAGATAATAAGGGTCAAACAGTGTTATGTCTGGGAGG
CTAAATGGCCTTAACTTGCAGGATACCTTATTAATTTTAATACTCTATACAATTCTTAAGTTATTTCACA
TTTGGTGCAAATCTGGAGCTGTAGACCAGTTCTGTAAATGTTGTATTAACCCTTCTCAAAAATGTGAAAT
ATTTTAATGGAGCCAAAGTGGTGATTTGCTTGGAAATAACACAATAACTTGGTTTGGAAGAAACCAATAA
GTGCTTAGTTTTCCTTGGGTGATCATTTAACTGGCAGCATATGCCAGCTTTCATGATCCAATAATGAACA
TGTTAAAAAAGTTTAAACATTATGGCTTAAAGTGAACACTGAATGACCATTACAGGTGTAGATTAGAAGA
CTGATTTCCTCAGATGCTAAAAAACCAAAATATTTCTGTCTTCCTGAATTGCCCTTTCAGAATTTCTGTT
CAGCTCTTTAGTCATTTGAGTTTACATTTGTAACATGAGGATATAATTGGATGTCTATTTGCTGAATGAC
CTATAACTTACCGCAGATGGTATCTTTTCTCCATTCTCCCAGATTTACACCTTCAAGCTCACAGGCAGCA
GCATATGCCTCTAAAATTTTGCAGAATTGTTCAGAATTCCCCTCTGTGGCACACAGGTCATAGACACAGC
TCTCAAAGTACAGCTGTGGTGGAATGTACCAATGGCAAGCCTCAAAAGGTCCACTATTTTTCAAAATAAT
TTTACAGATTTCTTCGTATTCTTTCTCTTTTCCTGTGTCACAGGTGGGTGGTACAACTGCAACTGGATTA
CATCTGTATTAAAAATAAACAGCAGTATACTTATATTTTGCTCAACCCTGCAAGGTAAACCTCAAGAGTA
ATAGAACTCTATGCCAAATTCTGTGGACTTATTGTGTATTCTTGCCTTTAGTTTGTCAAACCCCAAAGTG
AGAAATATTGTAATTCGACCCACAGTTCCCCATTAAGAAAACAATAGCGAACTGTTGAGATGTAACCACC
TGTCCATAGACAGAAGTCCAAATGGAATCAACCATCCAGATATTTAGCTCAGATAATTTCAGTTCACTAT
TGCCCATAGTAAGATCACTACTTTGCATATGAAACCATTAATAGGCAAAGAAATGCTCTGTGTCCCACTT
AGTCAATGTAGAATTACATTAGAAATAGAAGCTGAAGAAAATATTTAGCTTTCTTACAGTTAGATGGTAT
AAACAAGGTATTTTAAAATAGAAAGATAAGAAGAAATGAAGTTAACATCCAAGAAAAGAGCTTGATTTCA
AATAAACTTTATGTAGTTCATAACAAAAATCTTTGAAGGGTAATTTTAAAATTTCAGTGCTTTCTACTGA
AATGTAGAATTAGTAAAAAGGTTAAGATTCTAGCCAACATAATATCAAAAAAGGGATTCTATGGAATGCA
TATCATGTATTTTAGTTAATCTCTTAAGCAGATCTATGACATATTTTGTTGTTTCCTATAATATTATCAT
ATACTTACACCCAGTTGTCATCCTTCAGTAGCCAGCTGTCACCAAATTTATTTGGATCCTGTTCTAGGAC
TTTATCCGGTTTTAGAAAATCATCCAGCTGGTTGTCATTGAATGTCCCACACAAACCACATAACTGTCCC
CTGAGACTTTCATCAACCTGAATAAAAAGGTCCTGGTCGCCATCGAACTTCACCTGTATCCCTACAGAGG
TATCAATGACTACATAACGTCCTTTTACAGCTACTCTGGCTCCATTCTTCAAATCCACAGGAGGGAAAAC
CTGAGCTCCATCCACCTAAAGAAAAGAAAACCAACACAAATGGGTAAGTCAACAACGTTCCTAGGAAAAA
AAGGAGAGATCTACACTGATAATACTGCAAGTTTTCTTCATTGAGTCAGCCTTATCCTAAGGAGCAATGT
AGTCACAGAAGTTTGAAGGGACATTCATACTTGTCCCATATCTTGAATTCAGGTTTGTAGAACAACTTGA
GCTCAGCACTGTTGCAACTACACGTATAGCAATACTTGGAATAGCTAGTTTAAAGCCAGCTCTGGTATGT
AAGAGAGCATATAGCAAAAATGTTATTTCTGTTCTAAAGACCCAAACTGTAAAATGCAGATGGGCATTTA
GTTCAGCAAAAGCTTTCTATTAAGGATAAAGCTCCAGATGGAGCTGCTTTTTCTAAAATGTTGTGCATAA
AATATTTGAAAGGATATAATGAAGCTATGCTGATATCTACATTTCTGAAAAAATATTTCAGTGACTTCTT
TCATATATATTCTTGGAGGTGAATAAAGCAGAGAAACCTGATTCTGTTATTTTGGATGGAAAAATGCAGT
GCACTGATATGACTGATTATTTGTGCTTAAAGTGGAACACTTACAAAGGTCTCTTTGTCTCTCCTCACAA
CAATATGAAGATTTTTCAGTGTGACAGCAACTGAATCCAGGGCTGTCCAGTTTGGGTTTCCTCTATGCCT
ATTTCTGGTTGTCACTGAAAACCATTCAGAAGAGTTTGTGCAAGTCTCGACAACTGTATAATTGCAGTCT
CCTTGGAAATGATACAGTCTCCCATCAAAGGTTACGTAATGTGGATCCCCGAAAATGTGGCAGGTGCTGG
GATTTAATGGATAACACCCCAGGAGTCCATTCTGCACCTTGCAAACTTCATTTGCTTCGCATGTGTCTGA
ATTGCAAAGCACAGTCCCATTTCCAACACATATACATTGGCCTGCACAGTTTGTTTGCCAAAAAGTTTCT
CCTTTCTGTAAGAGAAGACAAAATAAAAAGAAGTTAAAACAGTTCATGTTAATGATCAAATGATCAAATG
TAAAATAAAACCTCTGAAATATGTGATAGAACAAACCTCATAATATTTGTCATTCTGTAAGCAGCCACAG
TTGCTCATAGATACACATTGTGCCCCACTGATGACAAAACCATCGTTGCATTCACATCCCTCTACACAAG
GTTTGCTGCAGTTGTTTGATGCAAGAGGGTCAGTGCAGGTTGCAGGACATGCACTGGTGCATGGATTGTA
ATGGCTGTTAGCAGGACATGCAATAGCTGAAAAAGAATAAAAGGCTCTCAAATGATAATTACAACTCACA
TCAACCCAAAATATGAAACTGAGATTATTTTAAAATACTGGCTATATTTTATCAGAAATATTATCAGAAT
ATAAAATTTATTTCCTGGTTTGAAATCAGAAAGAAAAATAATATTCTACAAGAGAAGGGCATGAATATTA
ACATATATTGTTGAAATTTAGCAAATTCAAATCAAAGAGAAAAATAAGTCACTCTATAACAGGTCCAGTA
CTTAGTTGGTACATGAATTATTGAAGCTATTAAATGTAGACAGGATACTAACAAACATATTAAAAGCAAA
ATATGTGATAAAACACAGAGGTGATTACATACTATCATGGGCTTACTGCTAACTCTATGACATAGATTCT
TTCAATAATGAGCACAGTTCTCGAAAGAGATCTGGCAGTAAAAAGAGTGGAGTGACCGTTGTAGAAGATT
ATTTCTCAGCATTTCATGGGGGAATGTAAGCAGAATTTAAGATTTCTAAGCTATGTCTGTTAACGCCTGA
AAGCAGACTTTTGAAAATGGAAGGAACTATTCTAAGATTCAGTTCTACATAGGTACTCACGGCAGAAGGT
GGCATTCCTCCAGGGGAGCAGAGTCACACCAGCTGCCTGGCATGCGTCAGCATAGGCCTCCAGCGCACCA
CACAGCACCTCCTGCGCACCACTCATGGCACAAAGGTCGTAGGAGCAGGTCTCAAAGTAGCTCTGCGGGT
TGATCACGGCGTGGCAGCTCTCAAAAACGCCTGGTCTGGCTGTCAGCATGCCACAGAACTGCTCGGACTC
GTAGAGCTTCTCCTTGTCTGCAGGGCAGGGCTCTGTTGGTGGGGGCACGCCGCAGCTGGGGTCACTGTCA
CTGCTTGGGACCTGCCAGCTCTCCCCCAGCGCATTGGAGTCTGCGGCTTGCTGCCCGTTGGGCATCATGT
AATCGTCCTCTCTCCGGTTGTTGAAGTTCCCGCACATGCCACACGTCAGGTTGAAGTAGCTGCTGGGCAC
CTTCACCTCCACCGAGTGGTCAGCGTCGTAGGACACGTGGAGGCTGAAGTCCGTCTCCAGGACGACGTAG
CGGCCGCTACTGCTCACGGTGATGGCTCCTCCTGCTGCGCTCACCGGCAGGGTCCGGTACTCACCGTTCA
CCTAGGGCAGACACAACGCAATGCACCGTGGACTCCCTGGGGCCGTCAGCCCCATGGGGACTGGCTCCCT
TCTCACACAGAAACTCCTGCTGCTTGCAGGTAAGACCCTGCCCAAAAAGACCAGCCTTGCCTTGCAGACC
CACCGCGCACACGCCAGACCCACTCCCTTACCAGCACTCGGCTCCTCTCCTGCTTGAGTATGGTAATGCG
CTCTCCGTACACATCGACCACGACTTCACGCACGTAGGACACTCGGGGGTTGCCACGATGCTCGTTCTTT
GCCTCAACGTTGAAGTACGGCACGGAGGTGTTGGAGCACACTTTGGCCAGGGTGTACGTGCAGTTCCCCA
TGAAGTTGTGCGTCACCTTGTCAAAGGTGTTGTAGTGAGGGTCCCCGTGGACGTGACAGATGCCGTAGGA
GTGTTCCAGGCACTCGGGTTTCCCATTCTGCACACCACAGAACTGGCCAGCAGGGCACGATGCACTGGAG
CACTGGACTTTGCCTCCTCGCGTGGGACATGTGCACTTGGACGAGCAGGTGTTGTCCGTCCAGAACTCTG
AGCCCACCGGGTAGTGCTGCCCGTTGTGCCAGCACCCACACTGCTGGCTGGGCACACACTGACTGTTGTA
GAGCAGGTACCCGCTGTCGCATACACATCCCTCCACGCACGGCAAGCTGCAGTTGTATGATGCGGTTGGG
TTAACGCATGTAGCAGGGCAAGCTGCAGCACAGGGCTCATAGTGGCTGTTGGCTGGGCAGGCTAGTGCTG
TGTAATGTAAAAGATTAATACAGAAGTTTATATTTTAAATATTGAAGTATTTGGGTTTATTTAATGGCAT
ATTCTGTTTTGTTCTGCAGTCTTGAGGACACCCTGGAATCCTGTGGCAATGCAATCCTTGCCTTTTCTGC
GTAAGTGCCCTTTAACATAGTTCATGGTTGTGGCTCCTAACAATTTCTCTGTCTGAGAATTTCTTATGAG
ATCTTCTATTCTGGTCATTGTTTGCTACTTTTTCCAGCTGAGGATATCTGTGTCATGTTATTTGTCCTTG
TCATGTGCCCCATTCAGGCTACAGGTCTGATAATTCTAATCATATCATAGCTTCTGAATTCATACTTTTT
CAATCCCTGAAGTTCTTTAATGTTTGTTAAATAATCGTGATGTACATGCACCCTATTCTGTTTTTATACC
TTGCTACACATGTCACTAGTTAAAAAGAAAAACTAAGCTAATTCGAAGTTCTTCTAACCTCAATCTTCAG
TCTCTTTTCAGATTCTGTACACTTATGTGATGTAGTTCAGTTTTTAATGACATGCCCCACTCTATTTTAG
TCTGCTTCCTTAAGCAATAGAGATCATATAATTACATTGTATATTTCCCTGTCCATCCTCTCATTAATTA
GAACTGACCCAGAAATAAGTGAGGTTGGCAGAGGACAAAGGCATAGTTTGAAACAGGAGTAAGGCAAGCA
GGAGAGACCCGCAAGCTCCTGCAGCAGAAAGAACAGCTACAGAGAGGGTTTATTCCTGCTTAGAAATCAC
GGAATTTATGTGGGAGTAAGCCTTAATAATATCCTTAGCAAATAAAATGCTCCAGCAAGAGATTACAATT
AATTGTGAAACAAATATCCAGAGTAAATTTGGCTTCAGCCATTTCAGTGTTTGCAGACCAGCATCTTTCT
TGAGTCTCCTCATCTCCCTCAAGGCAGAGGGCACACAGGCCACAGCAGAATATACTGATTCCCAGATCTT
CTGCTCAGTTCATCAAGCATTTTTCTGCCTCATCCTGCTCTCTTTACATTGGTATAAATTCTGAATTTCA
GACATCTTTACTGTTCAGTCTCAGATCTATTCCTCCCACCAGTTTTGATTTTATCATTCCTGCAGGGGCA
ACAAACTGACCCCCTGACTGGCTACCTAGTCCATGTTCCTTCTACTTAGTTTAGCCAGTCTCAACATGTA
CTTCCACTTTTCCATAGCCCTGTAAGCATGTTCTCGTTTTCCTTTCTAGAAGCTACTTGCCTTCTCAAAA
TCCAGGGCCTCCCATTTACCCCATTCAGCATTTCTCTCCAGTTCTTAATCAAAGCTCCTTTCTCATTGCC
CCAGACCACCTGGATTCCTTATCTACAGCAGCTCTGCCCTTCATCTGCTCTGGCTCATATCCCTTCTGTG
TTCACATGAGATATCTGTCTTAATGAGCTTCTGCTGGGGTTGCTGCAAGAGAAGGCACAACCTCCTTTCT
GTTGACTGGGTGCCTGGTCACCCTACAAGGTGAAGAGGCTAAGAGAATAGAGTAAGGTATCTCAGCTTCT
CTACAGGGGGCGTGGGTGAGGCTCTCACCTCTACTAAGCATGTGCAAAATGAGATTTTTTTTTTCCCCTC
AAAGACCCTAAAGTTTATGCACAGCTTCCTCAGAAGGTAAAAAACCCTCAAAGTCAAATGTCCCTGATAT
ACTTAGTACCCCTGAACAAAAATATAGGGTAGGAGAAGAATTCAAAAGAAAAGCTCTCTACTTTCATCAG
TCTTTCTAAAGGACTCAAGGATTCAAGCTAAGGACTACACATGATTCTGAAATCTTTAGCCTCATAGGTC
AAGATTTACAGTGTTACAGGAATTTAAAAGAGTTTCTTGTAATGCTCTCTCTTCAGGCAACCCTAATGAT
ATCCTCCTGACAAATGGTAATTTCCAGTAATCTTTGATACGTACGGCAGAAGGTTGCATTTCTCCATGGT
GTCAGCTGGACTCCTGCAGCTTGGCACATGTCTGCATAAGCCTGCAGGTTTTGACAGAGGGCTTCGCTGC
TCAAATGAAGCTCACAGAGATCATACTGGCAGTCTTCAAAATAGCTCTGTGGATCAACTACTGAGTGACA
ATTCTGGAATGGACCACTGGGATCAGTGATCAGACCACAGTATTTATTGCTTTGGATCATATGTTTTTCA
TCAGCAGTGCAATTTGGGATATGGCCATCATCTGGCAAGCACCTAGTGTGGAAAAAATAGATGTGGAAAA
TGTCACTAGTGAACTTTGCATACAATTGCACTCAAGATGCTTTTCAAATCCCAACTAATAAAACTACAGG
GCTGGCCCAGACTAAGGATGACTTTTAAAATATTTGTATTTCAAATGGGATGGGAATTCAGATTTTAAAA
TGATCTCAACATTATAACTTTTGTAATGCCTGTAAGAAAGTTAATCATTAATTTTGGTTTTAGGTCTAAA
AACAAGCTTTGTTCAAGTAAGACAACTAGGAAAGGCAAACAGAAAGTAAAAGCATTCAAAGTTAAACATC
TATCATCTTGAAATGACACACTGTATAATTATAAAATAAACAGTGAAGTATCTCAGAAGAGTTTTAGCAG
GATCATTAATATGCTAAAATAGATGGAGTCCTAGTGGGCCATTACAGGTGTATTCAATTTTTCTCTTTTT
AAGTACACTTGAATTTGCTACAAGTTTTTTTCTTCCCAGATACGTGAGAGAGCTATCCAGGCAAGAATAT
ATTTTAATCCATATTCCTGACATTTTGGGCCTACATACAATAGCTGCTTAAAATACTGCTCGTTACATAA
CTCCCAGATAAGGCCAAACATCCCAATTGGAGTCCTACCTTGAGTCATTGTAAACTTGCCAACTGTTTCC
AAGGCTGGCTGAGTCTGTTTCCATCCCTCCATCTGGGTTAAGAAAGTCATCTTTTTTGTCCCCATTATAA
TTTCCACATATCCCACAGACTTTTGATATATAGGCACTGGGAAGAGTGATTTCTGCTCTGTGATCTCCAT
CAAACTTGACCTTCAGCCCAAAGTCAGTAGTAACCACAACATATTGACCACTGAGGGAAATGCTGACACT
ATTGCCCAAGGTCAGAGGGAGTACCTGGCTGACTCCATCAACCTACAGGAACAGAGAAGGACAATCAGTA
TAATCACTTTAAAATACGTATTTTTAATAGTGATTTTCTTAGACCATGTTCCTATACAGAGCATAGGGTT
TGTACAGTGAACCTGTACTAGCATTAAGAACACACAGTGTTGTGTTTCTGGGTCTTTTTGGGCCTTACAT
AACATGAATGCTATATGTGGACAAATGACCTTGGGCCTCAAGACCGTAGTAATGCATGTACTTATCTTCC
AGGTCTTAGCTGTGGTCAAAGTAAGCTTGGTGAACTAGACCCAGCTGGAATAAGTGCAAAAATTATCAAG
GTCTTTCAGCAGTAACTGCTTACAGGCTTTCGGGATCAAACAAGAGCATGGTATAAGATTTAAAAGATGT
AGCTAGAATGGAGTCTCAGAAAATTTTCAGGGTTGGACATATTTTGAAAACATTTTTTGTTCCTTTTTTT
TTCTTTTTTTTTTCCCTCTTCTTTTTCTTTCTTGTTAATTTTCACATGTGAAATGCCAACAGTAAGAAGG
AAGTGTTCAAATAAAATTTGCAATTTATTTTAAATTCAATAAAACTAACATTCTTTGAATATTTGTTCTT
ATTACATAATTAATATATATACATTTCAACTTAGAAACATATATAATGAAAACAGTAAATACAGTGAAGA
GATTGAAAAGAGAAACTTTTGGACAAATGCAGATATCAAGTTCACTATGCATCTAAGTTGTGAAGTCTCT
GTATATTTAAATCCTTATCTCCTGTCCTATTTTCTTTATAAGAAGAGTGATAATGAAGTAAGGTGATCTA
TTCAATTACTGTGGGTTTTCAATCAAATGTGTATCTGCTACCTTGGCTCTTCATGGAAATAAGTTATAAG
GAAGCCTTAAAATACAGCAGCTGGTCCAGTGACACAATCAGAGTGATGTGAGTCATCAGGCAGCCTTTCT
GGGACTCTCATCTGGCTGTGCTTTATGGCAAGTGCCAGGTATTAAGTGAAATTAGCAGTGATGAGGAACA
ACTAGAAGTCAGAGATGAGCATGCAGCTTTACAGCCTGGGAAATATCTACCAGCACAAGCTCTGTTAATG
AAAGGCCTTTTTTATTCCCTGCTGCAGACCAAGTTTAGGAATGGAGGTCAATCTCTGAATGGAGGGCTCG
CTCACCAACTCCACCCAGAGTATCAGCTCTACTCTTAACTGTTAGGAACACAGAAATACAACGTTTTGTA
TTTTCTTTCTGTTCTGAAATCTCAATGCTTACTTTAAGGAACCCATAGTACTACTGTTCCCAAATATTTG
ACTGCCTTCTAAATATTGCTTTATGCTTGCCAAGAGATTTGTAGCCATTGTACTACAATTTCTTCAAAGC
ATGATTTACTGACAGTAGATAGTGAGATCCAGCCTTACCTTAACAACTCTGTTTTTACCCAGATTGATCC
TGTAGCCATAAACATCAATGTCCACAGATTGGACATAGGACACACGTTTGTTGCCTCTTCTGTGCTCGTT
AGCAGCTTCCACGTTGAAGTAGGGCAGTGAGCTGTTGTTCTCACACAGCTTGGAGAGCGTGTAGGTGCAA
GTGCCCATGAAGTCATGACGCTGCTTATCAAAAGTTAAATAATGAGGATCTCCATGTATAGTGCAAGTTG
CTTCTGGAGAAAGGAAAACAGAGTATGTTTCATTAAGATATTGGTGCATCCAGAAAAGCCAGCTCATACC
TTCGTTGTGCTGTGGGAAAACATAGTCATCCCCATTTTATAGGTTACACATGAAAGAACTGAAACAGCAA
AGACAGATTTTTATGGCCCAAGGCAATGCCACAGAAACACACTCAGATAAGTTAACTCAAGTAACACTGG
TAGAATGCACAGCTGCCCTTCTCTAAAGCACTGACTATCACATGTGATTTGTTAAAATTACCTGTCACAG
CAGGTGGTATAGGTGCCGTTGGTGTAGGTGTTGGTGGTACAGGGGTGCTGACTGCATCTGTCAGTAAAGA
CAAGGTAACAATAATTAGCATTATATAACATTACAAACTGAACACTACATACAAACCAACATACGTAACT
TTCAACTTACTCTTCACCAGTTTAAAGTTAGAATATCTTCACTGAAGAAAATGGAAGTCTACATTTAAAA
ATGAGGACTTTAGGTTCTTTTGAATTCTTAGGGAAGCTCATCTGACTACTAGCTTGGTGACAGTGACTGA
GCTTGTCCTTGCATGATTCTCTGAAACTGGAGATGTATTTACAATTGCATCTGTGATTCTCTCTGGTTTG
TATGTTAAAGCGTGAAGGTCATGTTCTCTGCAGGCCTTGGCAGGTCTGGAAATGAGTAGCCTGCCAGCAG
ATGCCATGTAACAGAAACAAGTGACTCTCAGGACACTGGTAGTTACTAATCAGTTTAATTTTTTCTACAA
AATGGCATTTATTTCTTAGGAATGTAATGAATGTAGGAGCTTTCTGCTGACAGATATTATAAACAGTGAT
GTTGAGTCTTTGCTGATGTCCATTACTGCTTGTTAATACATATTAATACATATTAGCATCTTCTCCACCT
ATTCACCTATCACAAAGACATATTCTAAAACAAGTGGTCCTTGTGGTGTATTAGTAAGTGTGTGCTAACA
ACCCCAAGGTGTACTGTAGGCATAGAGCTTAGCAGAAGGAGGAGCACTTCTCTAGTCTCTTGGGATTACA
TTAGTGCACCATCCGGTGGCTGAATTGTGTGGTAATAAGCCTGGAAAAATGAACAACTGGCTCTCCAAAT
GGGCCCCAAGTATTTTTCACAATTTTAAAGAAAATTACATCTTCTGAGGTTATAAACATGAATGGAACTC
TTCATTATCCAAAAATCCTAGCTAGAATAAATTTAAAACATTTCATTGACATTATATGAGTAGTGTTTTG
CAGAGATGTCTATTAAGACTTGATGATGTTCACAGCATGTTTCTTGTAGAATTCACATCCTGTGAGTTTT
GCATTCATAACAGTAAAGATGGGATTAAACTGTAATTCATTTCTGGCTTCAAGTCTTCCACAGACTGTGC
AACTCTAGACTGATTTATTAGAGGGTCAGAGATCAAACTCGAATTAGTGACGTATAATTAGGTATCACAG
TGCAAGTCAAAATTATTGATTCAACAATATACTTCTCTTTAATTACGCATGACAGACCTGTGTATTTTCT
GGCTCATACTGGACAGAGATTAGCTGTAAAAGATATTTAGAACACTGAAACCAGGAGTTGGACTCTATGA
TCCTTGTGTGTCCCTTCCATCTCAGGTTATCTTATGATCCAAGACCTTGGCTGGGTTTAGAGATGCACTT
CACACTTAAAGTTTGTTGGACCATGTGAAAATGGTCACATTTACAGAGAAGTGAATGGCTTAGTCTCTGT
GCTTGAGGCATTGAGGAAAGAACTGGGGAGAATTTACAATAAAAGAGGTAACATTTCTATGAAGTGAGCT
GGAAAGCCTTTTCCATTTCTCTTCTGCGGCTTGGAGTTATGAAGAGTTTCTATAACATAAATGGAATTGA
ATAATTGTCAACAGCTTAAAGAGAGATGAGGAAGGCACAAATATACACTTACCACAGGATCCATCTCTTA
CTCTCACATTGTCCAGTGCCGTATCTGCATACTCTGTCAGTCCTCGGACAGCCTCAAAAATGACCTAGGC
AGGATGCAAAGTCAATCGCTACATCAATTGTTCAGGCATATGTGAGAGAATTTCCCTCAACATGTTTTCA
GAATACTCTTTCTGGTCTATTTCTAAGGGCATCCAGGGAACCAATGGTGGAACACTTTCCCCAGTATTCT
TCAAGAAAAACTTGTAGAAGTTTTTTGTAGAAAAAGTAAGGCACCTAATGTCTTTGACATCATGATAAAA
TGAGGGAAGGAGGGAATGTGGGAAGGAAGGAAAGAGAGAGCAAATCAAATATATTCTATCTCCTAGCTAA
AAACACCAGTAAACCTCAGGGAGTTGTTTGTTTTTTCTCTTAGTTTATTTGCACAAAGCATGAGGGCATT
ATAATTACAAAATAGAAATCAAGAAAATTTTGGTTATTGTTTCTAGCTTATACATACAACCTTTTACAGC
CTGCAAAGGGAGAGCAAATCATAAATGCTGATGCTGTACAAACAAATTTATTATGAAAATATATTACCTT
TATCTTTCTCTGTGTTGGAAAAGTATGTGTGACAGCACCATAAATCCAAGAGGAACTTTGGTTTCCCTTC
CGACTCCATGCCGTTAACTCCCCTGTGCTGTCTTGGATAAGCACTCTCAGTTCATTCCTGTCTTCTGACC
CAAACATATAGTACCAGAAGTCTATGCATATCTTTTCAGAAACCACAATATCTGGGCTCTCAAGCCTGTT
AGTGTCGAATGGGATCAGATTGCTTGCTTCTTGGTAGATGAAATAGCCTTCTGCAGAGTGAAAAAAATAA
AAAAAATAAGTCTGAATCTTTCTAACTTGTTAGGTGTCACTTAAAACTGAGGTACATCTCAACTCTGGAA
GAAATCAAGCACATCATTAGCAGTACAAAGAAGCAAAGTAGCAAATGATTGTAATTTATACTGCAATGGT
TAGCACTGTGTAGTTAGCTAAAGTACAATGAGACCTGGAGAGCAAAGCAAATATATCCTATCTCATATGA
ACAGTTATTTTGGGATTGCAAATAAATATCTAGGCTTCTTTCAAAAGACAGCATTTCCAGACACAGTGCC
CAACTTATCCTTGTTCAATTTCCACTTTCCTCTGAGGAAAATAATGTGATCTACTATGCATCTGCTATAC
ACCTTCTCTTGCAAGATACGGTCTAATGATAAACAAATTTTTGGCATAAGGAAAATTCCAACCCCTCAAT
ATTTGGTTTTGTCTCAAAGCAGAGGGGTAAGAAAAAAAACACACAGATATTTCCATTAAAATGTTAGGTT
TGACATTATCAAAAGGAATCAATCTTTTCAAAAATCAGAATCTCACAATGTTTGATTTTGGGATTTAAAT
ACTTTACTTTGAACTTGTTAATTGTGAGATCACATTTTCCTTTACTAAGGCTGGAGCTTTTTGCTTCCTA
TCACTTCTCTCTGCTAAGATGAAAACTGTATTTTGCCAGTGCAGTTATGTCAAGTCTCTCCCATATGACT
ATGGAAAGGAGTAAAAAAGAAATGACCATATCTACATTATAAAGCATTACTCCACACACAGTATCTAATA
TTCAGTCTACTGTTTTAGGGCTCTTTTAATAATAAGAAAATGTAAGTAGCACTTGAAGTTATATGAACCA
TGCCAATTGAAAAAAATATATACTTATTGTAAGTGACCTTTCCAGTCTAGGGATAATTACTGAAAACAAT
ATGGCTACAGATTGATCTCCTATAAATGAATCTTCTAGTGCAAGGTTGAAGGTTCTACCATTCCATCTAG
TGATGGAGCCATATTAAACACTCTTTTAATGATAGGACCACATTAGACCTCAGTTTTTCAATCCCATGTA
GCTGAGGACGAGTTTTAAATGTATTCTTTGATTTCTGACTGTAATTTCCAATCTTGTAATGCTTTGCTAG
CATGAACATTATCCAGTGTCAGCACAGTGATCATTACATTTCTTGGCGTCAGCTGATATCACTGTAATTT
GATTATAAAACTTCTCCTGTGTAATGATCAATTGAATGTCACTGCATTCTCTGTGAGGTCTTAAGCAAGA
TGTAGTTAGCATGCTTCTACTTGCTTTTACTGCACTGATAAATATTTCCAACCTATGATGGGGAAAGCCA
GTTTAATCTTGTGACTGAACACGGTGAAAAGTCAAAGACAGGTGTCAACCAAACCGACACATTTCAAAGG
ATTTCATTAATCTCTTCATGAAAAAGAAAGAAGAAATGTAGACAAGCTGTATTCAGTAAACAATGAAGTA
AACAATGCCATCAACAAGCACTTACTTCCATCAGGATAGTCTCCATCAGGACCTGTTCCATCTGTTGGAG
TCTCATGCTTGGTTCGTATCCATACGCCCTGGTTCACAGTGCAAGGTTGGGTCCAGTCACAAAATGGTCT
TGAGTTGTTGTTAAAATCACATCGAGTGAGGTAAGCTGAAATGCAGAATAAGGTAAGTAAGGAAAAGGAG
ACACTATATTCAAAACCTGCCTTGGACATTAACAGCACTAAGTATAAACAGTTACCTGTTGCAGGATCCC
ATGATCTCAAGCCACCGGTTGGTCTGTGTTTGATCCTGCCAAGAAAAAGAACACAGTGATATCCCTACAG
ATTTAGTGACACAAAAACTGTTTAAGAGTGTAGAGACTAACAGAAGTCATCAGGTGTTCTTAATGAATCA
CTGCCCAGACTCAGGGTCTCATCTGTGTCTGACTCCTTCCCTTCCCATCTGTCCTTACTCCTTCATTAAG
AAGTTCTGAGAGCTAGAGAAAAAGTGTGATATGTTCACCTCATCTGAGTATAGAGGGATCCTCTCCCTGT
ATCTCTCTAAAGTGAAGGGAGAAAGTTCTACTAGGCACAGGGAGCAACAAGAGAGATGGCAAGGTACAGC
AAAGTTCGGATAATACCAGAATAACACAAAAGACTTGCTATTAACTTGTTTGTAGCACTGGCATCCTCAC
TACTGCTAAGCAAGATACGTAAACGCCAGTAAACTGGTGTGTCACAAAACTGTTATTTGTCAGAAGGCTT
ATGTTTCCTTTAACCACAACAAATATCTCATGACCACAAGCTGCCATCTCATTTAGTTTCTCCTGTAAGT
TATCCTAAAAATGCTACAGGGCAAGGGCAGTTAGAAATAGATATAGTCCCTTTGGTGCTAGAAAATCCTG
CAGAGGTGAGATGACATAAAACCAAAGCTCTGTCGCAGATCATAAGATGGCTCTATTCATTGGGTGTTTT
GCATCAGAAAGGGATTTCCAGGACTGCTAGACTGCAGTAGCTGCTCTTGACATGTTCTGATGGTGGTCTA
CAGCTTTAGGAGGCAGGGTCCAGTTCCTATCCCTGTGTTGCCCACCATCCCTACTGTCCTTGTTGTACCT
GACACAGTATAGAGAGAATCTGTCCTTAAGCAAAGCCATCACAGCAGATGTCCCCACAGCCCATGAAAAT
CTAAGCAGAGAGATTCAGAAAAACTAGGGGGATTCATAGTATCATATACAAACAGGATCTACTTCATCAC
TGAAACACACCAAACAACTGGAAAACAGACGAAGCTAAAGCTAAGGAGGAGAGAGGTGTGAAGATAGGTG
AAGTGAGAACATTTTCTTCTTTCCCCACAAGACAGCTGTAACAAGATGCCATTTAATCTCTATTCTATGC
TATCTTTAAACCGCTATATATCAAAGTCCTAAGGCAAAGGTTATTGTAGATATACTTAGAAAGTCACAAA
CATTTGATAACTGCAGCAGATCTCCCCTTTGGTTCAGCCATTAGAAATGTACAGTTTACACATTCTCTCA
CAGACAGCAACCACTGCAAAACTTGGCAATAACACAATTCAATTATTCCCTCCTTTTAAAAATAACATGG
TTTAATCGTACAGCATTCATGTGTAAAAAGATGAGAAAGGACATTTATAATTAACAATAACTACGTTTCC
TGAACTAAGGTAGATTCAGCATTGTGCTACTTACCCTGTGGTATTCAGCACCAAAATACAGAATAAAATC
AAAATGCATTGTAAGTGCCAGAGGGTCCCCATGCTAGAAGACAGTCAAGTCTTCTGTGTGGTACAGTCTA
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Molecule Role :
Vaximmutor
- Related Vaccine(s):
Mycoplasma gallisepticum lpd mutant vaccine
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IV. Vaccine Information |
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1. Avian Encephalomyelitis-Fowl Pox-Mycoplasma Gallisepticum Live Fowl Pox Vector, Live Virus Vaccine (USDA: ) |
a. Manufacturer: |
Biomune Company |
b. Vaccine Ontology ID: |
VO_0004206 |
c. Type: |
Live vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Chicken |
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2. Fowl Pox-Mycoplasma Gallisepticum Live Fowl Pox Vector Vaccine (USDA: 1D51.R0) |
a. Manufacturer: |
Biomune Company |
b. Vaccine Ontology ID: |
VO_0002201 |
c. Type: |
Live vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Chicken |
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3. M. gallisepticum TM-1 Protein Subunit Vaccine |
a. Vaccine Ontology ID: |
VO_0011515 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Gene Engineering of
TM-1 |
- Type:
Recombinant protein preparation
- Description:
- Detailed Gene Information: Click here.
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e. Adjuvant: |
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f. Immunization Route |
Subcutaneous injection |
g.
Chicken Response |
- Host Strain:
SPF
- Vaccination Protocol:
Eight-week-old specific pathogen-free (SPF) chickens were immunized subcutaneously in the right thigh with the TM-1 polypeptide, suspended in a mixture of 1 ml of PBS and 1 ml of an aluminium hydroxide adjuvant (Saito et al., 1993).
- Challenge Protocol:
Four weeks later, the immunized chickens were challenged intranasally with 0.5 ml of a suspension of 10^6 c.f.u, ml- 1 of M.g. KP13 strain 27 (Saito et al., 1993).
- Efficacy:
Only one of five immunized chickens harboured viable M.g. cells in its sinuses after being killed 5 days after challenge with M.g. (Saito et al., 1993).
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4. Mycoplasma Gallisepticum Live Culture Vaccine (USDA: 1751.00) |
a. Manufacturer: |
Intervet Inc., Lohmann Animal Health International, Merial, Inc. |
b. Vaccine Ontology ID: |
VO_0001702 |
c. Type: |
Live vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Chicken |
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5. Mycoplasma Gallisepticum Live Culture Vaccine (USDA: 1751.01) |
a. Manufacturer: |
Intervet Inc. |
b. Vaccine Ontology ID: |
VO_0001703 |
c. Type: |
Live vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Chicken |
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6. Mycoplasma gallisepticum lpd mutant vaccine |
a. Vaccine Ontology ID: |
VO_0002857 |
b. Type: |
Live, attenuated vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Chicken |
e. Gene Engineering of
lpd |
- Type:
Gene mutation
- Description:
This lpd mutant is from Mycoplasma gallisepticum (Gates et al., 2008).
- Detailed Gene Information: Click here.
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f. Immunization Route |
Intratracheal immunization |
g.
Chicken Response |
- Persistence:
An lpd mutant is highly attenuated in chickens (Gates et al., 2008).
- Efficacy:
An lpd mutant induces significant protection in chickens from challenge with wild type Mycoplasma gallisepticum (Gates et al., 2008).
- Host Gene Response of
IgG Fc fragment
- Gene Response:
Mg 7 vaccinated chickens mounted a strong humoral anti-M. gallisepticum-specific IgG response that was sustained for at least 4 weeks post-exposure. M. gallisepticum-specific immunoglobulin responses were detected in chickens inoculated with either Mg 7 or R(low) but not in chickens that received 1× PBS alone. The level of M. gallisepticum-specific serum IgG was highest in the R(low) treated animals at both the 2- and 4-week time points, however, the Mg 7 inoculated birds mounted a fairly robust response considering its level of attenuation. Thus, the Mg 7 vaccine induced significantly higher IgG responses than PBS inoculated chickens, but significantly lower than those inoculated with wild type R(low) (Gates et al., 2008).
- Detailed Gene Information: Click here.
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7. Newcastle-Bronchitis Mass Type, Killed Virus Vaccine-Mycoplasma Gallisepticum Bacterin (USDA: 48B5.10) |
a. Manufacturer: |
Lohmann Animal Health International |
b. Vaccine Ontology ID: |
VO_0002275 |
c. Type: |
Inactivated or "killed" vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Chicken |
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8. Newcastle-Bronchitis Mass Type, Killed Virus Vaccine-Mycoplasma Gallisepticum Bacterin (USDA: 48B6.10) |
a. Manufacturer: |
Wyeth, Intervet Inc. |
b. Vaccine Ontology ID: |
VO_0002276 |
c. Type: |
Inactivated or "killed" vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Chicken |
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9. Porcine Rotavirus Modified Live Virus Vaccine-Clostridium Perfringens Type C-Escherichia Coli Bacterin-Toxoid (USDA: 49C1.21) |
a. Manufacturer: |
Intervet Inc. |
b. Vaccine Ontology ID: |
VO_0002308 |
c. Type: |
Live, attenuated vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Pig |
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10. Porcine Rotavirus Modified Live Virus Vaccine-Escherichia Coli Bacterin (USDA: 49K1.20) |
a. Manufacturer: |
Intervet Inc. |
b. Vaccine Ontology ID: |
VO_0002315 |
c. Type: |
Live, attenuated vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Pig |
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11. Porcine Rotavirus-Transmissible Gastroenteritis Modified Live Virus Vaccine-Bordetella Bronchiseptica-Clostridium Perfringens Type C-Erysipelothrix Rhusiopathiae-Escherichia Coli-Pasteurella Multocida Bacterin-Toxoid (USDA: 49T9.21) |
a. Manufacturer: |
Intervet Inc. |
b. Vaccine Ontology ID: |
VO_0002319 |
c. Type: |
Live, attenuated vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Pig |
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12. Porcine Rotavirus-Transmissible Gastroenteritis Modified Live Virus Vaccine-Clostridium Perfringens Type C-Escherichia Coli Bacterin-Toxoid (USDA: 49B1.21) |
a. Manufacturer: |
Intervet Inc. |
b. Vaccine Ontology ID: |
VO_0002307 |
c. Type: |
Live, attenuated vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Pig |
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13. Porcine Rotavirus-Transmissible Gastroenteritis Modified Live Virus Vaccine-Escherichia Coli Bacterin (USDA: 49P1.20) |
a. Manufacturer: |
Intervet Inc. |
b. Vaccine Ontology ID: |
VO_0002318 |
c. Type: |
Live, attenuated vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Pig |
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14. Vaxsafe MG |
a. Tradename: |
Vaxsafe MG |
b. Manufacturer: |
Bioproperties |
c. Vaccine Ontology ID: |
VO_0001181 |
d. Type: |
Live, attenuated vaccine |
e. Status: |
Licensed |
f. Host Species for Licensed Use: |
Chicken |
g. Immunization Route |
Intramuscular injection (i.m.) |
h. Description |
Live temperature-sensitive mutant strain; eye drop administration(Barbour et al., 2000) |
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V. References |
1. Barbour et al., 2000: Barbour EK, Hamadeh SK, Eidt A. Infection and immunity in broiler chicken breeders vaccinated with a temperature-sensitive mutant of Mycoplasma gallisepticum and impact on performance of offspring. Poultry science. 2000; 79(12); 1730-1735. [PubMed: 11194034].
2. Gates et al., 2008: Gates AE, Frasca S, Nyaoke A, Gorton TS, Silbart LK, Geary SJ. Comparative assessment of a metabolically attenuated Mycoplasma gallisepticum mutant as a live vaccine for the prevention of avian respiratory mycoplasmosis. Vaccine. 2008; 26(16); 2010-2019. [PubMed: 18342996].
3. Merck Vet Manual: M. gallisepticum: Merck Veterinary Manual- Mycoplasma gallisepticum Infection [http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/203402.htm]
4. Saito et al., 1993: Saito S, Fujisawa A, Ohkawa S, Nishimura N, Abe T, Kodama K, Kamogawa K, Aoyama S, Iritani Y, Hayashi Y. Cloning and DNA sequence of a 29 kilodalton polypeptide gene of Mycoplasma gallisepticum as a possible protective antigen. Vaccine. 1993; 11(10); 1061-1066. [PubMed: 8212828].
5. Wijesurendra et al., 2017: Wijesurendra DS, Kanci A, Tivendale KA, Devlin JM, Wawegama NK, Bacci B, Noormohammadi AH, Markham PF, Browning GF. Immune responses to vaccination and infection with Mycoplasma gallisepticum in turkeys. Avian pathology : journal of the W.V.P.A. 2017; 46(5); 464-473. [PubMed: 28345962].
6. Wiki: Mycoplasma gallisepticum: Mycoplasma gallisepticum [http://en.wikipedia.org/wiki/Mycoplasma_gallisepticum]
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