VIOLIN: Vaccine Investigation and Online Information Network

Streptococcus equi

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. binding protein Eag (Protective antigen)
2. cell surface protein (Protective antigen)
3. collagen binding, ancillary pilus subunit Cne (Protective antigen)
4. Fbp (Protective antigen)
5. Fnz (Protective antigen)
6. fnz (Protective antigen)
7. Glyceraldehyde-3-phosphate dehydrogenase GAPDH (Protective antigen)
8. HAP (Protective antigen)
9. putative surface-anchored 5-nucleotidase (Protective antigen)
10. sclC (Protective antigen)
11. scpZ (Protective antigen)
12. Sfs (Protective antigen)
13. sfs (Protective antigen)
14. spaZ (Protective antigen)
15. surface-anchored protein (Protective antigen)
16. szm (Protective antigen)
17. SzP (Protective antigen)
18. vicK (Virmugen)
Vaccine Related Host Genes
1. IgA
2. IgG
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

1336

2. Disease:

Strangles (also equine distemper)

3. Introduction

Strangles (also equine distemper) is a contagious, upper respiratory tract infection of horses and other equines caused by a bacterium, Streptococcus equi var equi. The disease is spread when the nasal discharge or material from the draining abscess contaminates pastures, barns, feed troughs, etc. Clinical signs include fever, heavy nasal discharge, and swollen or enlarged lymph nodes in the neck and throatlatch. Affected animals may also stop eating and have a dull affect. Mortality may be up to 1 in 10 horses affected with strangles, especially bastard strangles. For cases without complications, mortality is usually lower. The disease is very contagious and morbidity is high. Precautions to limit the spread of the illness are necessary and those affected are normally isolated. An isolation period of 6 weeks is usually necessary to ensure that the disease is not still incubating before ending the isolation (Wiki: Strangles).

4. Microbial Pathogenesis

S. equi enters via the mouth or nose and attaches to cells in the crypt of the tonsil and adjacent superficial lymphoid nodules. After a few hours, the organism is difficult to detect on the mucosal surface because it is translocated below the mucosa into the local lymphatics where it may be found in one or more of the lymph nodes that drain the pharyngeal/tonsillar region. Complement-derived chemotactic factors generated after interaction of C1 with bacterial peptidoglycan attract large numbers of PMN. The inability of PMN to phagocytose and kill the streptococci appears to be due to a combination of the hyaluronic acid capsule, antiphagocytic M protein, and a leukocidal toxin released by the organism. This culminates in accumulation of many extracellular streptococci in the form of long chains and large numbers of degenerating PMN. Final disposal of these organisms is dependent on lysis of the abscess capsule and evacuation of its contents (Timoney, 2004).

5. Host Ranges and Animal Models

S. equi primarily is a pathogen of equids, but can also cause disease in cattle, rabbits, swine, sheep and humans (MicrobeWiki: S. zooepidemicus). Mice can be used as a model of disease (Flock et al., 2006).

6. Host Protective Immunity

Most horses develop a solid immunity during recovery from strangles which persists for up to five years in about 75% of animals. Acquired resistance appears to be primarily humoral and mediated by antibodies to SeM and other as yet unidentified antigens unique to S. equi (Timoney, 2004).

II. Vaccine Related Pathogen Genes

1. binding protein Eag

Gene Name : binding protein Eag
Sequence Strain (Species/Organism) : Streptococcus equi subsp. equi 4047
VO ID : VO_0011211
NCBI Gene ID : 7696154
NCBI Protein GI : 225870123
Locus Tag : SEQ_0721
Genbank Accession : FM204883
Protein Accession : YP_002746070
Other Database IDs : CDD:144825
Taxonomy ID : 553482
Gene Starting Position : 711797
Gene Ending Position : 713086
Gene Strand (Orientation) : ?
Protein Name : Ig, alpha2-macroglobulin and albumin binding protein Eag
Protein pI : 4.57
Protein Weight : 41524.96
Protein Length : 429
Protein Note : B domain; pfam01378

DNA Sequence : Show Sequence

>NC_012471.1:711797-713086 Streptococcus equi subsp. equi 4047, complete genome
AATGGAAAAAAACAAAAAGGTATCCTACTTTTTACGTCAATCAGCTGTTGGTTTAGCTTCAGTGTCGGCA
GCCTTTCTGGTTGGAACGACTTCAGTGGGTGCTTTAGACGCAGCAACAGTGTTAGAGCCTACAACAGCCT
TCATTAGAGAAGCTGTTAGGGAAATCAATCAGCTGAGTGATGACTACGCTGACAATCAAGAGCTTCAGGC
TGTTCTTGCTAATGCTGGAGTTGAGGCACTTGCTGCAGATACTGTTGATCAGGCTAAAGCAGCTCTTGAC
AAAGCAAAGGCAGCTGTTGCTGGTGTTCAGCTTGATGAAGCAAGACGTGAGGCTTACAGAACAATCAATG
CCTTAAGTGATCAGCACAAAAGCGATCAAAAGGTTCAGCTAGCTCTAGTTGCTGCAGCAGCTAAGGTGGC
AGATGCTGCTTCAGTTGATCAAGTGAATGCAGCCATTAATGATGCTCATACAGCTATTGCGGACATTACA
GGAGCAGCCTTGTTGGAGGCTAAAGAAGCTGCTATCAATGAACTAAAGCAGTATGGCATTAGTGATTACT
ATGTGACCTTAATCAACAAAGCCAAAACTGTTGAAGGTGTCAATGCGCTTAAGGCAGAGATTTTATCAGC
TCTACCGAGTTCTGAGGTCATTGACGCAGCAGAACTAACACCAGCCTTGACTAGCTATAAGCTTGTCATC
AAGGGAGCAACTTTTTCAGGTGAAACAGCTACTAAGGCAGTAGACGCAGCTGTAGCTGAGCAGACCTTCA
GAGACTATGCTAATAAAAATGGTGTAGACGGCGTTTGGGCTTATGATGCTGCCACAAAGACATTTACAGT
CACTGAACAGCCTGTAGCTGAGACTATTGAGGCGGCAGAATTAACACCAGCTTTGACCACCTATAGGCTT
GTTATTAAGGGGGTTACCTTCTCAGGTGAAACAGCTACCAAGGCAGTAGACGCAGCCACAGCCGAGCAGA
CCTTCAGGCAATATGCCAATGACAATGGCGTTACTGGTGAATGGGCTTATGACGCTGCTACAAAGACATT
TACAGTCACTGAGGCTTTAGAGGAGAGTCCAGCTGATCCAGAAAAGCCATCAGCTTCTCTTCCGTTGACA
CCGCTTACACCAGCAACTAAGACAGCACCAGCTAAGCAAAAAGATAAGCAAAAGGCTAAGACTCTTCCAA
CGACTGGTGAGAAGGCTAATCCATTCTTTACAGCAGCGGCTCTTGCCATTATGGCGAGTGCAGGTGCTTT
AGCAGTGACTTCAAAGCGTCAGCAGGACTA

Protein Sequence : Show Sequence

>YP_002746070.1 Ig, alpha2-macroglobulin and albumin binding protein Eag [Streptococcus equi subsp. equi 4047]
MEKNKKVSYFLRQSAVGLASVSAAFLVGTTSVGALDAATVLEPTTAFIREAVREINQLSDDYADNQELQA
VLANAGVEALAADTVDQAKAALDKAKAAVAGVQLDEARREAYRTINALSDQHKSDQKVQLALVAAAAKVA
DAASVDQVNAAINDAHTAIADITGAALLEAKEAAINELKQYGISDYYVTLINKAKTVEGVNALKAEILSA
LPSSEVIDAAELTPALTSYKLVIKGATFSGETATKAVDAAVAEQTFRDYANKNGVDGVWAYDAATKTFTV
TEQPVAETIEAAELTPALTTYRLVIKGVTFSGETATKAVDAATAEQTFRQYANDNGVTGEWAYDAATKTF
TVTEALEESPADPEKPSASLPLTPLTPATKTAPAKQKDKQKAKTLPTTGEKANPFFTAAALAIMASAGAL
AVTSKRQQD

Molecule Role : Protective antigen
Molecule Role Annotation : Vaccination of mice with EAG (α2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein) as a single antigen with EtxB (as an adjuvant) induced protection in mice against bacterial challenge, but was less protective than at affording protection than immunization with FNZ, SFS, EAG, and EtxB (Flock et al., 2004).
The antigens used were EAG (alpha2-macroglobulin, albumin, and IgG-binding protein), CNE (a collagen-binding protein), and SclC (a collagen-like protein). The vaccinated horses showed significantly reduced bacterial growth (p=0.02).(Waller et al., 2007)
Related Vaccine(s): S. equi Subunit EAG, FNZ and SFS Protein Vaccine

2. cell surface protein

Gene Name : cell surface protein
Sequence Strain (Species/Organism) : Streptococcus equi
NCBI Protein GI : WP_043026814
Other Database IDs : CDD:303769
CDD:293650
CDD:302792
CDD:293879
CDD:295219
CDD:279132
Taxonomy ID : 1336
Protein Name : cell surface protein
Protein pI : 10.11
Protein Weight : 71332.09
Protein Length : 719
Protein Note : Guanylate-binding protein, C-terminal domain; cl20817

Protein Sequence : Show Sequence

>WP_043026814.1 cell surface protein [Streptococcus equi]
MITKKQRKQKRHTTIRRLCHQALAATLITGSLMGTGLLGNKVVYGDVKKDADNIKKVLEENKGKLSEELK
KVTDFNTLLVLFSILSGDVSLSSSNLSSAITTFSDLVNSFNKQENNRNQANREALFSEISRRISALDEAT
RNVMKTKQELKMTIQDLESEKLKKQLLNSELDKTREAYKLVIKTSNELEKKLNKATMDKKQAEEKAKKET
AAKEKAEKDTKTAKQESEQANQRAEKAEAASKKAEDAAKKATEEANAAKEIAHQAQEKSKQLEQATQQAN
QKAEQAEKAKKALETQKEKLEQEIKQAQKSKTEAEQKLKELQESSMKNDTAAQQLLKEKEELTTKLQDLQ
KQAKEKTTEIEKLKQELEANKQNSGQLNQKEKELQEQLNKVQKELKQKEMELEKAKEQLKQEQKPHEGGG
DSDASKARITELEKQVQTLTKEKESLSSTLESTKAQLSETQAKLSEAQKQLTATQEKLTTLEAEKAALQK
QVDTISKQLSEARDLSEKEKAALQEQINKLKAEIEQKTKEIEALKQGMHSHQGQEKPKDPKTPETPKDPK
TPEKNDQPQAPEKRSVPWTALTPAKPIDTTKAPKSSAPSPQTGAATPKKQLPATGDTATPSFFTAAAMAV
LASVGVLNLSPRRKKNQNNR

Molecule Role : Protective antigen
Molecule Role Annotation : (Fu et al., 2013)

3. collagen binding, ancillary pilus subunit Cne

Gene Name : collagen binding, ancillary pilus subunit Cne
Sequence Strain (Species/Organism) : Streptococcus equi subsp. equi 4047
VO ID : VO_0011213
NCBI Gene ID : 7695900
NCBI Protein GI : 225870316
Locus Tag : SEQ_0935
Genbank Accession : FM204883
Protein Accession : YP_002746263
Other Database IDs : CDD:283410
CDD:212461
CDD:273478
Taxonomy ID : 553482
Gene Starting Position : 914185
Gene Ending Position : 916158
Gene Strand (Orientation) : ?
Protein Name : collagen binding, ancillary pilus subunit Cne
Protein pI : 6.61
Protein Weight : 68135.68
Protein Length : 657
Protein Note : Collagen binding domain; pfam05737

DNA Sequence : Show Sequence

>NC_012471.1:914185-916158 Streptococcus equi subsp. equi 4047, complete genome
TTTGAAACAACTGACAAAGATCGTTAGTGTGGTCTTGTTGCTGGTCTTTACCCTTAGTGCTAGCCTGCAC
AAGGTTCGGGCAACTAATCTTAGTGACAACATCACATCATTGACGGTTGCTTCTTCATCACTCCGAGATG
GAGAGAGAACGACGGTAAAGGTTGCGTTTGATGACAAAAAACAGAAAATCAAGGCAGGGGATACGATAGA
GGTCACCTGGCCTACAAGTGGTAATGTCTACATTCAGGGCTTTAATAAAACCATACCGCTTAATATTAGA
GGGGTAGATGTTGGTACCTTGGAGGTCACGCTAGACAAGGCTGTTTTCACATTCAATCAAAATATTGAAA
CAATGCATGATGTCTCTGGTTGGGGAGAGTTTGATATTACTGTTAGAAATGTGACACAAACCACCGCTGA
AACATCAGGAACGACCACAGTAAAGGTAGGCAATCGCACTGCTACTATCACTGTTACTAAGCCTGAGGCA
GGCACTGGTACCAGCTCATTTTATTATAAGACTGGTGATATGCAGCCCAATGATACTGAGCGTGTGAGAT
GGTTCCTGCTGATTAACAACAACAAGGAATGGGTGGCCAATACTGTTACAGTCGAAGACGATATTCAAGG
TGGTCAAACCTTGGATATGAGCAGCTTTGACATCACCGTATCTGGTTATCGTAACGAGCGCTTCGTTGGG
GAAAACGCTCTGACAGAGTTTCATACAACATTTCCAAATTCTGTCATTACGGCAACAGATAATCACATTA
GTGTGCGGTTAGATCAATATGATGCCTCACAAAACACTGTCAACATTGCTTATAAGACAAAGATAACGGA
CTTTGACCAAAAAGAATTTGCCAACAACAGTAAAATCTGGTACCAGATTTTATACAAGGATCAGGTATCG
GGTCAAGAGTCAAACCACCAAGTAGCCAATATCAATGCTAACGGCGGGGTTGATGGCAGTCGCTATACCA
GCTTTACTGTCAAGAAAATTTGGAATGACAAGGAAAATCAAGACGGTAAGCGTCCAAAGACTATTACTGT
TCAGCTTTACGCCAATGATCAGAAAGTTAATGATAAGACCATTGAATTGAGTGATACTAATAGCTGGCAA
GCAAGTTTTGGTAAGCTGGATAAGTATGACAGTCAGAACCAAAAAATTACCTACAGTGTCAAGGAAGTGA
TGGTTCCTGTTGGCTACCAATCGCAGGTTGAGGGGGATAGTGGAGTAGGATTTACCATTACCAACACCTA
TACACCAGAGGTCATTAGCATTACCGGTCAAAAAACTTGGGACGACAGGGAAAACCAAGACGGTAAACGT
CCTAAGGAGATTACGGTTCGTTTATTGGCAAATGACGCTGCAACTGACAAGGTAGCAACTGCTTCAGAGC
AAACCGGCTGGAAGTATACATTTACCAATCTACCGAAATACAAAGATGGTAAACAGATCACCTACACGAT
CCAAGAGGACCCTGTGGCAGATTACACCACAACCATTCAGGGATTTGATATTACCAATCATCATGAGGTA
GCCTTGACCAGCCTAAAGGTCATCAAGGTTTGGAATGATAAGGACGATTATTACCATAAACGTCCCAAGG
AGATTACCATTTTGCTAAAGGCAGATGGCAAGGTGATTCGTGAACATCAGATGACACCGGATCAGCAAGG
AAAATGGGAATACACCTTTGACCAGCTGCCGGTCTATCAGACAGGCAAGAAAATCAGCTACAGCATTGAG
GAAAAACAGGTTGCTGGCTATCAAGCCCCTGTCTATGAGGTTGATGAAGGCTTGAAGCAGGTCACTGTAA
CCAACACCCTTAACCCAAGCTACAAGCTGCCTGACACCGGAGGACAAGGAGTGAAATGGTACCTGTTAAT
CGGTGGCGGTTTTATCATCGTCGCAATCCTTGTACTGATCAGCCTTTATCAAAAACACAAGCGCCATAAC
ATGTCAAAACCATA

Protein Sequence : Show Sequence

>YP_002746263.1 collagen binding, ancillary pilus subunit Cne [Streptococcus equi subsp. equi 4047]
MKQLTKIVSVVLLLVFTLSASLHKVRATNLSDNITSLTVASSSLRDGERTTVKVAFDDKKQKIKAGDTIE
VTWPTSGNVYIQGFNKTIPLNIRGVDVGTLEVTLDKAVFTFNQNIETMHDVSGWGEFDITVRNVTQTTAE
TSGTTTVKVGNRTATITVTKPEAGTGTSSFYYKTGDMQPNDTERVRWFLLINNNKEWVANTVTVEDDIQG
GQTLDMSSFDITVSGYRNERFVGENALTEFHTTFPNSVITATDNHISVRLDQYDASQNTVNIAYKTKITD
FDQKEFANNSKIWYQILYKDQVSGQESNHQVANINANGGVDGSRYTSFTVKKIWNDKENQDGKRPKTITV
QLYANDQKVNDKTIELSDTNSWQASFGKLDKYDSQNQKITYSVKEVMVPVGYQSQVEGDSGVGFTITNTY
TPEVISITGQKTWDDRENQDGKRPKEITVRLLANDAATDKVATASEQTGWKYTFTNLPKYKDGKQITYTI
QEDPVADYTTTIQGFDITNHHEVALTSLKVIKVWNDKDDYYHKRPKEITILLKADGKVIREHQMTPDQQG
KWEYTFDQLPVYQTGKKISYSIEEKQVAGYQAPVYEVDEGLKQVTVTNTLNPSYKLPDTGGQGVKWYLLI
GGGFIIVAILVLISLYQKHKRHNMSKP

Molecule Role : Protective antigen
Molecule Role Annotation : Mice were immunised i.n. with collagen-binding protein (CNE) and were subsequently subjected to infection with S. equi subsp. equi. A control group of mice was given only adjuvant. The CNE vaccinated group had a delayed onset of infection compared to the control group. Immunization with CNE led to significant protection from infection (Flock et al., 2006).
The antigens used were EAG (alpha2-macroglobulin, albumin, and IgG-binding protein), CNE (a collagen-binding protein), and SclC (a collagen-like protein). The vaccinated horses showed significantly reduced bacterial growth (p=0.02).(Waller et al., 2007)
Related Vaccine(s): S. equi Subunit CNE Protein Vaccine

4. Fbp

Gene Name : Fbp
Sequence Strain (Species/Organism) : Streptococcus equi subsp. equi 4047
VO ID : VO_0011216
NCBI Gene ID : 7697172
NCBI Protein GI : 225871286
Locus Tag : SEQ_2017
Genbank Accession : FM204883
Protein Accession : YP_002747233
Taxonomy ID : 553482
Gene Starting Position : 2043360
Gene Ending Position : 2044964
Gene Strand (Orientation) : -
Protein Name : antiphagocytic cell surface-anchored fibrinogen-and IgG Fc-binding protein SeM
Protein pI : 9.36
Protein Weight : 55929.26
Protein Length : 534
Protein Note : Previously sequenced as Streptococcus equi fibrinogen-binding protein Fbp UniProt: AF012927 (534 aa" target="_blank">O68165 (EMBL: AF012927 (534 aa) blastp scores: E()=0.0

DNA Sequence : Show Sequence

>NC_012471.1:2043360-2044964 Streptococcus equi subsp. equi 4047, complete genome
CTTAGTTTTCTTTGCGTTTAGGAGACACCACAGCCATACCAGCACCAGCGATGACAGTAAGAGCTGCAAT
AGTGAAGAATGGGTTAGCAGACTCACCAGTAGATGGCAATTGACCTGCTTTAGCAGTTGTAGTGCCTGGG
TTTGTTTGTGAAGCACGACCACCTTGTGACAATGACTTAATCCTGTCAGCTAGCTCTTGCTTCAAGCGAT
TGATTTCTGCTTGAAGATCTGCATTTGCATGGTCTTTCTTCTCAAGCATTTCTTTAAGTTTTGAAAGTTC
CTCAGCATGAGCTTTTTTGAGCTCTTCAATCTTAGCTTCAAGCGCTTCTTTTTCCTTCTTAACGGCTTCA
AGCTCTTCTTCTTTCTTAGCTAATTGTACTTTAAGATCTGCAACCTCAGCTTTAGCAGCTTCAACTTCTT
TTTCAAGCTCTGCTACCTTAGCATCTGATGCTGCTTTTTGTTTTTCAAGCTCTGCTACATTAGCTTGTGA
TTCTACTAAAGCCTTATCAGAAGCATCTTTTTGTTTTTGTAGCTCAGCAATTTCACGTTTGCCTGATTCA
ATCAAACGCTCTGAAACAGCAAGCTCTGTCTTAGCTTTTTCTAGCTCTGTTTGAAGTGATGCAATGGTGT
CATTTGCAGCTGCAAGCTCTGCATCAGCCTTAGCCTTATCAGCAGTTAATTGATCAATTTCTGCTAATTT
TTGAGCCATTTCACGTTTTGCAGACTCTACCAAGCGGTCTAGACTACCAACGTTTTCCATTAACTTTTCA
GCTTCTGCTGTTTTAGCTGCCAATTTATCTGCTAAATCTTTTGATACTGCAAAAGCTTTATCACGACTTG
CTTCAGCACTACTTAGTTTATCTGCTAAATCTTTTGATACTGCAAAGGCTTTATCACGACTTGCTTCAGC
ATTGCTTAGCTTATCTGCTAATTCCTTAGTCATCTTAGCTGCTTTTTCTTTTTCTTTTGCAAGTTCTGTT
TTTAGTTTATTATACTCTTTTGTTGATCTTGATAAAGTTTTGTTGAATGATTCGGTAAGAGCTTGACGCT
CATCTTTAGCTTTTTGTAGTTCTTGTTCTTTTTCCTTCTCTAGATTTCGAAGTTGGGAATCTCTTTCTCT
AAGCATTTCTTGTTGAGATTTAATAGCTGCAAGTTTAGCCTCAAGAAGTTTTGCATAACTATCTCCATCA
GCAATCCGCTTTTCAATTTCATCTACAAGTAATGAAGCCAAACTTAATTGTCTTCTATCCCCATCAGGTT
TATCATTTAACATCGATGAAAGGTGCACCAATAAATTGTAATAATCATCTCTACCATACGCAGCCCTTGC
TGAATCAAGACCTCTCAATAATGCCTGTAAATCCCCAACAGAGGCGCCTTTTAGAAGATTTCGAACTTTT
TGGGCTGATGAGGCATCTCTACTTATGGCTATTTCGCTTAATCTATTTTTTAAATCACGCGATAATCTTG
GAGTCGCCGTACGACTAACCTCAGAGTTCGCTTTTACAGTTGTCCCTCCCAACACACTTGTTGCAACTAA
TACTGATGCTGCACCGGCACTTAGTTTTCTGATGCTAAATTTTTGCTTGTTATTTCTCAAAAACA

Protein Sequence : Show Sequence

>YP_002747233.1 antiphagocytic cell surface-anchored fibrinogen-and IgG Fc-binding protein SeM [Streptococcus equi subsp. equi 4047]
MFLRNNKQKFSIRKLSAGAASVLVATSVLGGTTVKANSEVSRTATPRLSRDLKNRLSEIAISRDASSAQK
VRNLLKGASVGDLQALLRGLDSARAAYGRDDYYNLLVHLSSMLNDKPDGDRRQLSLASLLVDEIEKRIAD
GDSYAKLLEAKLAAIKSQQEMLRERDSQLRNLEKEKEQELQKAKDERQALTESFNKTLSRSTKEYNKLKT
ELAKEKEKAAKMTKELADKLSNAEASRDKAFAVSKDLADKLSSAEASRDKAFAVSKDLADKLAAKTAEAE
KLMENVGSLDRLVESAKREMAQKLAEIDQLTADKAKADAELAAANDTIASLQTELEKAKTELAVSERLIE
SGKREIAELQKQKDASDKALVESQANVAELEKQKAASDAKVAELEKEVEAAKAEVADLKVQLAKKEEELE
AVKKEKEALEAKIEELKKAHAEELSKLKEMLEKKDHANADLQAEINRLKQELADRIKSLSQGGRASQTNP
GTTTAKAGQLPSTGESANPFFTIAALTVIAGAGMAVVSPKRKEN

Molecule Role : Protective antigen
Molecule Role Annotation : The fibrinogen-binding protein (FgBP) reacted with serum taken from horses recovering from strangles and protected mice against lethal challenge from S. equi subsp. equi (Meehan et al., 1998).

5. Fnz

Gene Name : Fnz
Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus
VO ID : VO_0011214
NCBI Gene ID : 7695217
NCBI Protein GI : 754170878
Locus Tag : SZO_RS08670
Genbank Accession : FM204884
Protein Accession : WP_041790586
Taxonomy ID : 40041
Gene Starting Position : 1860617
Gene Ending Position : 1862326
Gene Strand (Orientation) : +
Protein Name : thioester-forming surface-anchored protein
Protein pI : 4.75
Protein Weight : 58938.07
Protein Length : 569
Protein Note : Ortholog of S. equi 4047 (FM204883) SEQ0375

DNA Sequence : Show Sequence

>NC_012470.1:1860617-1862326 Streptococcus equi subsp. zooepidemicus H70, complete genome
TATGGAGAGAAGAGAGTTGAAAACAAAATCATTTAGAAAGGTACTGACGACGTCAGCTACCTGTATTGTG
CTGGCAACAAGCTTTGCCGGAGGAACCCTACGCGTCTGGGCAGAGCAGCTTTATTATGGGTGGGATGATG
GAACGAGACATGATTCACCCTCCTTTTTATATGTAACGCCTACTACTGAAAGTCAAAGAAGCCCCAAAAC
TATTGTATACTGTTTTAATCGAAACAATTATTGGCCGGATAATTGGGAAACCCATGTCTTAAAACCAGAT
AATCTACCTACTTATAATAAATTAACAGGAACTGCGAAGTTATTTCAAAATAAAGCTACCAAACCAAGAT
CGGTAGCAGATATCGAGAAGTCCTTAGTTTCTGTCCTAAGTAAAGGGTATCCAACAAGTAATCTCTCTCA
ATTTGGCATCAGTAATGAGTCAGCTAGAATAGTGACACAATTAGCCATTTGGTATTTTAGTGATAGTGTG
AACGATCCTGAAAGCGTACTTAGACAAAGATTAGGGGGCAATGAATCTAAAGCATTAAAATACCTTATTG
AGGTTGGTGAAAAACCACAATTGAGCGAAGCTGAGCAAAAGCAAACGTTAGATATATACGTTTACAACTC
AGGAGGTTATCGTAGAACAGGTTACCAAAATCTTCTCGGCTCTACCTTAATTCCTAAAACACCAGAAAAG
CCTTCTTCTGAATCTGTAACTCAGAGTAACCTTATTCTACTTGAACAGGAGAATCATTCTGGTACTATTT
CAGGTGGTTCAGGAAATACTGAAACTGAGGAGAATACTCCTCATCTAATGGGGATAGGTGGCGGTCTAGC
TGGTGAATCAGGAGAAAACACACCTAAACCAGGGGAAACTGGTGGGCACTCCCCTATAGTTGAAGAAAAC
TACGGTAGTACTGAAGAATTTCATGGGAATTCAGAAATTATTAGTGAAACCGAAGATACTAATCCTGGTA
TCATTTTAGGTGGCTCAGGAAATGTCGAAACACATGAAGATACTAGGAATCCTCATCTGATGGGGCTTGG
CGGTGGTCTAGCTGGCGAATCAGGAGAAACGACACCTAAACCAGGACAGACTGGTGGTCATGGACCAATC
ATCGAGACAACAGAGGACACACAAAAAGGCATGTCTGGGCAATCTGGCGGCACTATCGAGTCAGAAAACA
CCAAAAAGCCTGAGGTCATGATTGGTGGGCAGGGACAAACCATCGAAACAACAGAGGACACACAAAAAGG
CATGTCTGGGCAATCTGGCGGTACTATCGAGTCAGAAGACACTAAAAAACCTGAGGTCATGATTGGCGGT
CAGGGACAAATCATCGACTTCTCTGAAAACACGCAATCAGGTATGTCTGGACAGTCTGGAGACACTACGG
TCATTGAGGATACCAAGAAGTCTGAGATAATCATTGGTGGGCAAGGACAAATCATCGACTTCTCTGAGGA
TACTCAGCCGGGTATGTCTGGGCAATCTGGAGGCACCACAATTGTCGAAGACACCAAGAAGCCGACACCT
AAGCCTAAACCTGCACCTGCGCCAATTGTTAATGACGAAAAACCTAACAAAGGCACTCAGCTCCCACAGA
CAAATGATATGAAGCAACTCACCCTAAGCATCATCGGTGCAATGTCAATGCTGCTTGTCCTATGTCTGTC
TCTATTCAAGCGACCATCTAAAAAAGACTA

Protein Sequence : Show Sequence

>WP_041790586.1 thioester-forming surface-anchored protein [Streptococcus equi]
MERRELKTKSFRKVLTTSATCIVLATSFAGGTLRVWAEQLYYGWDDGTRHDSPSFLYVTPTTESQRSPKT
IVYCFNRNNYWPDNWETHVLKPDNLPTYNKLTGTAKLFQNKATKPRSVADIEKSLVSVLSKGYPTSNLSQ
FGISNESARIVTQLAIWYFSDSVNDPESVLRQRLGGNESKALKYLIEVGEKPQLSEAEQKQTLDIYVYNS
GGYRRTGYQNLLGSTLIPKTPEKPSSESVTQSNLILLEQENHSGTISGGSGNTETEENTPHLMGIGGGLA
GESGENTPKPGETGGHSPIVEENYGSTEEFHGNSEIISETEDTNPGIILGGSGNVETHEDTRNPHLMGLG
GGLAGESGETTPKPGQTGGHGPIIETTEDTQKGMSGQSGGTIESENTKKPEVMIGGQGQTIETTEDTQKG
MSGQSGGTIESEDTKKPEVMIGGQGQIIDFSENTQSGMSGQSGDTTVIEDTKKSEIIIGGQGQIIDFSED
TQPGMSGQSGGTTIVEDTKKPTPKPKPAPAPIVNDEKPNKGTQLPQTNDMKQLTLSIIGAMSMLLVLCLS
LFKRPSKKD

Molecule Role : Protective antigen
Molecule Role Annotation : Parts of the three recombinant proteins (FNZ (cell surface-bound fibronectin binding protein), SFS (secreted fibronectin binding protein), and EAG (alpha2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein)) were used to immunize mice, either subcutaneously or intranasally, prior to nasal challenge with S. equi subsp. equi. The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin. It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) (Flock et al., 2004).
Related Vaccine(s): S. equi Subunit EAG, FNZ and SFS Protein Vaccine

6. fnz

Gene Name : fnz
Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus MGCS10565]
NCBI Protein GI : ACG61689
Other Database IDs : CDD:285533
CDD:274864
CDD:281046
Taxonomy ID : 552526
Protein Name : fibronectin-binding protein Fnz
Protein pI : 4.97
Protein Weight : 60736.07
Protein Length : 682
Protein Note : Fibronectin-binding protein signal sequence; pfam08341

Protein Sequence : Show Sequence

>ACG61689.1 fibronectin-binding protein Fnz [Streptococcus equi subsp. zooepidemicus MGCS10565]
MERRELKTKSFRKVLTTSATCIVLATSFAGGTLRVWADKYYGWNDGTRRDSPFFLYVSPQNDPKRELKDE
CIVYCFNRDKQWPETWGVNRTFSQVQSEVTFKLPLYEKKKGSDEVFKNNAPRFRGTVKNVTAALVAVLKS
GYPTVKSINHLDAEKSRKVTQLAIWYFSDSENDPDTYLQPSEKLTQEEKTAFNSLVEEGKKAGETLSSTG
DYTLDIYITTDKNYAQKQYQNLLGSHVIPKKDDTPNLHGSGPKLNGEIGGKTELPNSIGGESRYPNDEKG
EAGPPATPQKPNDPLQGLEGGNSPIVEQNYGSTEGYHGQSGILEETEDTNPPGIILGGSGNVETHEDTRN
PHLMGIGGGLAGESGETTPKPGQTGGHGPIIEITEDTQKGMSGQSGGTIESENTKKPEVLIGGQGQTIET
TEDTQKGMSGNSGGTIESEDTKKPEVLIGGQGQIIDFSENTQSGMSGQSGDTTVIEDTKKSEIIIGGQGQ
IIDFSEDTQPGMSGQSGGTTIVEDTKKPTPKPKPAPAPIVNDEKPNKGTHLPQTSEFKQLTLSIIGAMSM
LLVLCLSLFKRPSKKD

Molecule Role : Protective antigen
Molecule Role Annotation : (Flock et al., 2004)

7. Glyceraldehyde-3-phosphate dehydrogenase GAPDH

Gene Name : Glyceraldehyde-3-phosphate dehydrogenase GAPDH
Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus SzS31A1
NCBI Protein GI : EQB24294
Taxonomy ID : 1352602
Protein Name : glyceraldehyde-3-phosphate dehydrogenase
Protein pI : 4.97
Protein Weight : 32411.54
Protein Length : 445

Protein Sequence : Show Sequence

>EQB24294.1 glyceraldehyde-3-phosphate dehydrogenase [Streptococcus equi subsp. zooepidemicus SzS31A1]
MVVKVGINGFGRIGRLAFRLIQNVEGVEVTRINDLTDPAMLAHLLKYDSTQGRFDGDVEVVEGGFNVNGK
FVKVSAEANPENIDWASQEVDIVLEATGFFASKAGAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHEIL
DGTETVISGASCTTNCLAPMAKVLQDQFGVVEGLMTTIHGYTGDQMTLDAPHRKGDFRRARAAAVNIVPN
STGAAKAIGLVIPELNGKLDGAAQRVPVPTGSVTELVSVLEKEVTADEVNAAMKAAANDSFGYTEDQIVS
SDVVGIHYGSLFDATQTKVLTVDGKQLVKTVSWYDNEMSYTAQLVRTLEYFAKIAK

Molecule Role : Protective antigen
Molecule Role Annotation : (Fu et al., 2013)

8. HAP

Gene Name : HAP
Sequence Strain (Species/Organism) : Streptococcus equi subsp. equi 4047
NCBI Protein GI : 4336671
Other Database IDs : CDD:226636
CDD:173869
Taxonomy ID : 553482
Gene Strand (Orientation) : ?
Protein Name : hyaluronate-associated protein precursor
Protein pI : 5.08
Protein Weight : 53754.78
Protein Length : 622
Protein Note : ABC-type oligopeptide transport system, periplasmic component [Amino acid transport and metabolism]; COG4166

Protein Sequence : Show Sequence

>AAD17886.1 hyaluronate-associated protein precursor [Streptococcus equi subsp. equi 4047]
MTVLGTKACKRLGLAAVTLASVAALMACGNKQSASTDKKSEINWYTPTEIITLDISKNTDTYSALAIGNS
GSNLLRADAKGKLQPDLAEKVDVSEDGLTYTATLRDGLKWSDGSDLTAEDFVYSWQRMVDPKTASEYAYL
ATESHLKNAEDINSGKNPDLDSLGVKADGNKVIFTLTEPAPQFKSLLSFSNFVPQKESFVKDAGKDYGTT
SEKQIYSGPYIVKDWNGTSGTFKLVKNKNYWDAKNVKTETVNVQTVKKPDTAVQMYKQGKLDFANISGTS
AIYNANKKHKDVVPVLEATTAYIVYNQTGAIEGLNSLKIRQALNLATDRKGIVSAAVDTGSKPATALVPT
GLAKLSDGTDLTEHVAPGYKYDDKEAAKLFKEGLAELGKDALTITITADADAPAAKSAVDYIKETWETAL
PGLTVEEKFVPFKQRLEDTKNQNFEVAVVLWGGDYPKGSTFYGLFKSGSAYNYGKFTNADYDAAYNKALT
TDALNTDAAADDYKAAEKALYDNALYNPLYLP

Molecule Role : Protective antigen
Molecule Role Annotation : Immunization with HAP-GST significantly reduced rhinitis in Balb/C mice challenged nasally with S. equi and significantly increased survival time and clearance of bacteria in CBA/CA mice challenged intraperitoneally with S. zooepidemicus (Chanter et al., 1999).

9. putative surface-anchored 5-nucleotidase

Gene Name : putative surface-anchored 5-nucleotidase
Sequence Strain (Species/Organism) : Streptococcus equi subsp. Zooepidemicus
NCBI Protein GI : CAW99100
Other Database IDs : CDD:223808
CDD:301300
CDD:277317
CDD:280945
EnsemblGenomes-Gn:SZO_08800
EnsemblGenomes-Tr:CAW99100
GOA:C0MEK3
InterPro: IPR004843
InterPro: IPR006146
InterPro: IPR006179
InterPro: IPR008334
InterPro: IPR019931
InterPro: IPR029052
UniProtKB/TrEMBL: C0MEK3
Taxonomy ID : 40041
Protein Name : putative surface-anchored 5'-nucleotidase
Protein pI : 4.64
Protein Weight : 66099.44
Protein Length : 775
Protein Note : Signal peptide predicted for SZO08800 by SignalP 2.0 HMM (Signal peptide probability 1.000) with cleavage site probability 0.986 between residues 27 and 28

Protein Sequence : Show Sequence

>CAW99100.1 putative surface-anchored 5'-nucleotidase [Streptococcus equi subsp. zooepidemicus]
MKKHFVLKSSVLGVFAGVSMLVTGVQADQVDVQFLGVNDFHGALDTTGSAYMPEGKVSNAGTAAQLAAYL
DDAEADFKQTSPDGTSIRVQAGDMVGASPANSGLLQDEPTVKVFNQMGFEYGTLGNHEFDEGLAEFNRIM
TGTAPAEDSAINDITKQYEHEASNQTLVIANVVDKKTNTIPFGWKPYAVKEIPVNDKSVKVGFIGVVTTE
IPNLVLKQNYEQYDYLDSAETIVKYAKELQEQQVNAIVVLAHIPATSDNGVVHDEMAAVMEKVNQLYPEH
SVDIVFAGHNHQYTNGTIGTTRVVQAVSQGKAYADVRGTLDTDLNDFVATPEAKVIAVAPGIKTGNADIQ
AIVDQANDIVKTVTELKIGTAATSSTISKTENEDKESPVGNLITTAQLTIGRKTFPHVDFAMTNNGGIRA
DLVVKEDQTITWGAAQAVQPFGNILQVVQMTGQQIYDVLNQQYDEDQKYFLQMSGLKYTYTANEANDPST
PFKVVKAYKDNGEEIDPAAQYTLIINDFLYGGGDGFSVFKDAKLLGAINPDTEVFIEYIKDLEAAGKTVS
AGINGVKTYVTASLEGSTQSDHTGKHEIINRVFKNREGKVLSTELVSDLFTPAQNANPATSIKPLAAKKQ
ANAALPATGTSQHSAAAMTILGLLTLTGTSLFKKKEGQ

Molecule Role : Protective antigen
Molecule Role Annotation : (Wei et al., 2012)

10. sclC

Gene Name : sclC
Sequence Strain (Species/Organism) : Streptococcus equi
NCBI Protein GI : AAV70833
Other Database IDs : CDD:333130
CDD:307064
Taxonomy ID : 148942
Protein Name : collagen-like protein C
Protein pI : 9.94
Protein Weight : 30137.67
Protein Length : 377
Protein Note : group: C

Protein Sequence : Show Sequence

>AAV70833.1 collagen-like protein C [Streptococcus equi subsp. equi]
MTNKTKRTGLVRKYGACSAAIALAALASLGAGKAVKADQPAALKYPEPRDYFLHTREGDVIYDEDIKRYF
EDLEAYLTARLGGIDKKVEEAAQKPGIPGPTGPQGPKGDKGDPGAPGERGPAGPKGDTGEAGPRGEQGPA
GQAGERGPKGDPGAPGPKGEKGDTGAVGPKGEKGDTGATGPKGDKGERGEKGEQGQRGEKGEQGQRGEKG
EQKPKGDQGKDTKPSAPKAPEKAPAPKAPKASEQSSNPKAPAPKSAPSKSAAPTGQKAALPATGEINHPF
FTLAALSVIASVGVLTLKGKKD

Molecule Role : Protective antigen
Molecule Role Annotation : (Waller et al., 2007)

11. scpZ

Gene Name : scpZ
Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus MGCS10565]
NCBI Protein GI : ACG62304
Other Database IDs : CDD:173801
CDD:278510
CDD:239048
CDD:283851
CDD:304423
Taxonomy ID : 552526
Protein Name : C5a peptidase precursor ScpZ
Protein pI : 4.77
Protein Weight : 115524.31
Protein Length : 1234
Protein Note : Peptidase S8 family domain in Streptococcal C5a peptidases; cd07475

Protein Sequence : Show Sequence

>ACG62304.1 C5a peptidase precursor ScpZ [Streptococcus equi subsp. zooepidemicus MGCS10565]
MEVLLVTKKYHSKKSLLCQLTLATTSILLLHSQTVFAEEQQGSDASVSQASPVVAAEATATGDDSQAAAS
NQAASETEAKTEKEISAFAEGTESTAPTTLSVHDPANAQPLQEQTQKGSGAVIAVIDVSFDPEHDAFKLD
SDLKNNGTIPAKEAFLAKKQDQHITYGKWYNEKIIFAYDYTKQSDDIGGLTADNGHGNHTAAIAAGNGKT
PAGNGLRVEGIAPNASLMLMKVTGAPERDQFAKSYAKAITDAVNLGATVISMSFGKTADSLSTVHEDVKK
AMAYAQEKGVLLVAGAGNESAVGMGTREPLAANPDFGTLNSPAIFEEVISVAASNPVYAISQSVNAKTAD
GSQKLAIFMSEGDSFDADQDYFVADAKFGEASHFNGSDVKGKVALIERGGSLSFYQKIENAVKAGASGVI
IYNNNALEGSFTIEKASIPKEARIPVGFMSYKDAQALKAGHSFTFNKAYEKMPSNAGGRVISQSSWGVTA
EGRIKPDISAPGVNVYSAVHGNQYDYKTGTSMSTPMVSGLVAMLHKAYKEKFPELSAQELSQLVRAVLMS
SARTLYSAENKAYISPRQQGAGEVDGQKALAASYYLTDQKQNPKINLGNITDEFVINLNVNALSKNQGPK
KLYFQVNLITDQTKDGHFTLQPKALKDSEWQEINITEDQQHIQVSVDAKAYAAELLKAMPNGYFLEGFVR
FTDNLDTKEELMSIPFSGFRGDFANLPALDTPIYETLEQGAFYQKLEQDPETGKYILPEKFSKLTALIGQ
VSPYFLSQDTKNGMVNELGPEAERYIILGTQESADQKDLIAKEADRTFLISPNDDGNKDFILFQGVFLRS
VKDIKAQVLDHQGQVVWESDVATAQKYYQEGVVLPYRFEHTKWSGKDANDQPVADGSYTYRVWYTPIADG
AEAQKQDFKVQVKTSLPELPKSASYDESTRTLKIDASNFPAYRIQVGHIVEIGEGEEADIVVNYFPMAED
GTVIIPKTVTSELSGEEVEVDMAALKVIVEDEFGNFNAIALADLLKQKEPEIKDDKQPEPETPMPEQPKG
NDSSKEQQSPKGNNSGSKQPLVMKQDSAVKPMTSSPSHELPKTSEKQGSSLLAGLGMLLASLSLGLFKKK
SDN

Molecule Role : Protective antigen
Molecule Role Annotation : (Wei et al., 2013)

12. Sfs

Gene Name : Sfs
Sequence Strain (Species/Organism) : Streptococcus equi subsp. equi 4047
VO ID : VO_0011215
NCBI Gene ID : 7696331
NCBI Protein GI : 225869898
Locus Tag : SEQ_0466
Genbank Accession : FM204883
Protein Accession : YP_002745845
Taxonomy ID : 553482
Gene Starting Position : 452509
Gene Ending Position : 453624
Gene Strand (Orientation) : ?
Protein Name : fibronectin-binding protein Sfs
Protein pI : 8.97
Protein Weight : 39068.51
Protein Length : 371
Protein Note : Previously sequenced as Streptococcus equi fibronectin-binding protein Sfs UniProt: AF136451 (371 aa" target="_blank">Q9X6F4 (EMBL: AF136451 (371 aa) fasta scores: E()=1.8e-153, 100.000% id in 371 aa

DNA Sequence : Show Sequence

>NC_012471.1:452509-453624 Streptococcus equi subsp. equi 4047, complete genome
TATGAGAAAAACAGAAGGACGTTTTCGCACATGGAAGTCCAAAAAACAATGGCTATTTGCCGGTGCAGTG
GTGACCAGCTTGCTGCTGGGAGCTGCACTTGTCTTTGGAGGTTTATTAGGAAGTCTTGGTGGCTCATCCC
ATCAAGCGAGGCCAAAGGAGCAGCCAGTCAGCTCGATTGGAGATGACGATAAGTCGCACAAGAGCTCATC
AGATTCAATGGTCAGTCGGCCACCGAAAAAGGATAACTTGCAGCCTAAGCCTTCAGATCAGCCTACTAAT
CATCAGCATCAGGCTACTAGCCCGTCCCAGCCGACAGCAAAGAGCTCAGGTCATCATGGGAATCAACCTC
AGTCTCTCAGTGTGAACAGCCAAGGAAATAGTAGTGGACAGGCCTCAGAGCCTCAGGCTATTCCTAATCA
AGGGCCTTCCCAGCCACTGGGGCTGAGAGGAGGTAACAGCTCTGGTTCAGGTCATCACCATCAGCCACAA
GGAAAGCCACAGCACTTAGATCTAGGTAAGGATAATTCTAGCCCGCAGCCTCAACCAAAGCCTCAGGGCA
ATAGTCCCAAGCTGCCAGAAAAAGGCTTGAATGGTGAAAATCAGAAGGAACCGGAGCAAGGTGAACGAGG
TGAGGCTGGTCCCCCACTTTCAGGGTTGAGTGGTAATAATCAAGGCCGTCCTTCGCTTCCAGGCTTGAAT
GGTGAGAATCAGAAGGAACCAGAGCAAGGTGAACGAGGTGAAGCCGGTCCCCCATCAACTCCGAATTTAG
AAGGAAATAATCGTAAAAATCCTTTAAAAGGATTAGATGGAGAGAATAAGCCAAAGGAAGATTTAGACGG
TAAAGGCTTAAGCGGAGAAAATGATGAATCACCAAAACTTAAAGACGAACACCCCTACAATCATGGTCGT
CGTGATGGTTACCGAGTTGGCTATGAGGATGGATATGGTGGCAAAAAGCACAAAGGAGATTATCCTAAGC
GGTTTGATGAGTCTAGTCCAAAGGAATATAATGACTATAGTCAAGGGTATAATGATAATTATGGAAATGG
CTATTTAGATGGTCTAGCTGATAGAGGTGGTAAGAGAGGATACGGCTATTCTTACAATCCCGACTA

Protein Sequence : Show Sequence

>YP_002745845.1 fibronectin-binding protein Sfs [Streptococcus equi subsp. equi 4047]
MRKTEGRFRTWKSKKQWLFAGAVVTSLLLGAALVFGGLLGSLGGSSHQARPKEQPVSSIGDDDKSHKSSS
DSMVSRPPKKDNLQPKPSDQPTNHQHQATSPSQPTAKSSGHHGNQPQSLSVNSQGNSSGQASEPQAIPNQ
GPSQPLGLRGGNSSGSGHHHQPQGKPQHLDLGKDNSSPQPQPKPQGNSPKLPEKGLNGENQKEPEQGERG
EAGPPLSGLSGNNQGRPSLPGLNGENQKEPEQGERGEAGPPSTPNLEGNNRKNPLKGLDGENKPKEDLDG
KGLSGENDESPKLKDEHPYNHGRRDGYRVGYEDGYGGKKHKGDYPKRFDESSPKEYNDYSQGYNDNYGNG
YLDGLADRGGKRGYGYSYNPD

Molecule Role : Protective antigen
Molecule Role Annotation : Parts of the three recombinant proteins (FNZ (cell surface-bound fibronectin binding protein), SFS (secreted fibronectin binding protein), and EAG (alpha2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein)) were used to immunize mice, either subcutaneously or intranasally, prior to nasal challenge with S. equi subsp. equi. The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin. It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) (Flock et al., 2004).
Related Vaccine(s): S. equi Subunit EAG, FNZ and SFS Protein Vaccine

13. sfs

Gene Name : sfs
Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus MGCS10565]
NCBI Protein GI : ACG61768
Other Database IDs : CDD:274741
Taxonomy ID : 552526
Protein Name : fibronectin-binding protein Sfs-like
Protein pI : 8.85
Protein Weight : 36230.61
Protein Length : 442
Protein Note : KxYKxGKxW signal peptide; TIGR03715

Protein Sequence : Show Sequence

>ACG61768.1 fibronectin-binding protein Sfs-like [Streptococcus equi subsp. zooepidemicus MGCS10565]
MRKTEGRFRTWKSKKQWLFAGAVVTSLLLGAALVFGGLLGSLGGSSHQARPKEQPVSSIGDDDKSHKSSS
DSMVSRPPKKDNLQPKPSDQPTNHQHQATSPSQPTAKSSGHHGNQPQSLSVNSQGNSSGQASEPQASPNQ
GPSQPLGLRGGNSSGSGHHHQPQGKPQHLDLGKDNSSPQPQPKPQGNSPKLPEKGLNGENQKEPEQGERG
EAGPPSTPNLEGNNRKNPLKGLDGENKPKEDLDGKGLSGENDESPKLKDEHPYNHGRRDGYRVGYEDGYG
GKKHKGDYPKRFDESSPKEYNDYSQGYNDNYGNGYLDGLADRDGKRGYGYSYNPD

Molecule Role : Protective antigen
Molecule Role Annotation : (Flock et al., 2004)

14. spaZ

Gene Name : spaZ
Sequence Strain (Species/Organism) : Streptococcus equi
NCBI Protein GI : ACG61542
Other Database IDs : CDD:330553
CDD:307064
Taxonomy ID : 552526
Gene Strand (Orientation) : ?
Protein Name : protective antigen-like protein, fibrinogen- and Ig-binding protein precursor
Protein pI : 4.83
Protein Weight : 41874.96
Protein Length : 541
Protein Note : RecF/RecN/SMC N terminal domain; cl25732

Protein Sequence : Show Sequence

>ACG61542.1 protective antigen-like protein, fibrinogen- and Ig-binding protein precursor [Streptococcus equi subsp. zooepidemicus MGCS10565]
MKSCKSTLRKAAMAAMLAGAITTTGGVKAEENFDFYNHVMQFLEDKSDDESYEKLGKAFEKLPEEQMKAL
FRIWFSADNSDGISQSVMSIAHKLKEKEQLEFKEVGEMLWNSVTDELDRRIDSEASLKKDKVELQDNLNK
AIKVSTDLMNQLGAAKADRDKAQAEADRLSQAVDSLGRLTNSAKRELTEKLAEIDQLTQDKAKAASDLET
ANANITTLQEELAGAKASYDKLSEDKAAADQALAEANVRVSELEQQVAAANNRVVDLEAQLAEKTAELDM
VKADKAELEAKVKELQSLLEQKDRSNADLQANIERLKQELADKFKDQAQPRAKTPQASNLVAKQKASRPA
AKPAGQLPSTGEDAANPFFTAAAVAIMTGASLVTVMPKRQKD

Molecule Role : Protective antigen
Molecule Role Annotation : (Wei et al., 2012)

15. surface-anchored protein

Gene Name : surface-anchored protein
Sequence Strain (Species/Organism) : Streptococcus equi
NCBI Protein GI : KIS15526
Taxonomy ID : 1381082
Gene Strand (Orientation) : ?
Protein Name : surface-anchored protein
Protein pI : 4.68
Protein Weight : 105684.48
Protein Length : 1089

Protein Sequence : Show Sequence

>KIS15526.1 surface-anchored protein [Streptococcus equi subsp. zooepidemicus Sz4is]
MEQRKFKKILALVLTVLTMLSSLSFGTNAVNANGTDKYVGWSEYQGSYSAAFYLGKENSTDRYVAYCYNK
KREAPPRKIDGTVYYQKTGGTPQEYGQSLYHPMTDQQKEETLIKDLRRVLYNGYPHNKGKLSEQFSSVPE
VQRDGLFREVTQWGVWHYTDGFDPEVYYKKDFELASGSAKQMNELKVKVYKTLIGQGLQEADSSFEMDLF
TTSQTSQETGNRYQNILVTKDNMPLPKPPDTPYGREEKIYITKVGVINGMIERNALSDAKLEIREGEGLT
GTALASNITIYPDQFEFSGFEFDLRTYGKRFGDVFTLVETQAPPGYKKSEPIVFMVAGVDELGGDYWIQI
KQPDGTWKQQELPDKSKVQDGDDLDENSLKQTLIVYDEKQNPEYDITFKKMDETGDYLDGAEFALYKEDD
PTKAIKTFITGKQDINLKLEAGNYILRERSAPTGYVGVEEDIKFTVNNQGQVTAHGSTPEGVTFNAQSIE
CKNKKEVAEETVTLKVKKLWDNQEKAEHPETKFGLFKDGVLQIDKVQTLSNGMTEITWENIKKSEYQSYQ
PFELIEKDGQLEKAGKYITLEDKTYEVFSDLEWKDEGNNVYSIKINNYLTNIITIDKEWQDKDGRKLEDD
LPSINIHIEMDNGAREDKTFDTDNTSNEYYISATLKIESFFEENVKLQLNNQVEVTVDGKVYLATLTQTS
ENHYKVVNKEKALGHVVKISKKSGSETGAELKDATLKVFREADDETTAQPIKEWKTKETPEELLLEEGSY
KLVESEAPQGYRKAETIHFTVNDDGTIAVTGQQLQPGEPIVMVDKAIQTVTIEKKVTGVNGNKQKEFNFK
LTVNADDDLKNGEVLDGKLYKDGQVLDVNIMVGREYLFNLKDTDKLEVTGLVAESTYTLNEDEYNKDGYT
TTVMVNDKEKKNADTDATYTVVDGKNNVIFINHNEKIVPTGILFDIMPYLIGMAFVTLSSIAFLLSVKRR
IVLKNAKK

Molecule Role : Protective antigen
Molecule Role Annotation : (Wei et al., 2012)

16. szm

Gene Name : szm
Sequence Strain (Species/Organism) : Streptococcus equi subsp. Zooepidemicus
NCBI Protein GI : AKL88101
Other Database IDs : CDD:273479
CDD:302998
CDD:290581
CDD:295219
CDD:287215
CDD:279132
Taxonomy ID : 40041
Gene Strand (Orientation) : ?
Protein Name : Szm
Protein pI : 5.64
Protein Weight : 56605.01
Protein Length : 608
Protein Note : Gram-positive signal peptide, YSIRK family; TIGR01168

Protein Sequence : Show Sequence

>AKL88101.1 Szm [Streptococcus equi subsp. zooepidemicus]
MFLRNKKQKFSIRKLSAGAASVLVATSVLGGAAVEFTSGTIYAEEKIVEADSAKFFDLMVEKKLYDEFSR
FGYGKKPEEVLASLKQVLKDASPQTLNSLINTLDNGRIDYQTGMGQVFSQAFGSFRADHDPEYLAYSFYS
LLIQAIKERVVNESELKVQLQEKEEAVEVLTKILDKTVNEKIEIDRKAKEAEGKLKESSLKLTKAYKITK
DLADQLSNVKQQRDKAFKVSKDLADKLSNAEASRDKAFAVSKDLADKLSSAEASRDKAFAVSKDLADKLA
AKTAEAEKLMQNVGSLDRLVESAKREMAEKLAEIDQLTADKAKADAELATANDTIASLQTELEKVKTELA
VSERLIESGKREIAELQKQKDASDKALAESQANVAELEKQKAASDAKVAELEKEVEAAKAEVADLKAQLA
KKEEELEAVKKEKEALEAKIEELKKAHAEELSKLKEMLENKDHANADLQAEINRLKQELADRIKSLSQGG
RASQANPGSTTAKAGQLPSTGESANPFFTIAALTVIAGAGMAVVSPKRKEN

Molecule Role : Protective antigen
Molecule Role Annotation : (Velineni and Timoney, 2013)

17. SzP

Gene Name : SzP
Sequence Strain (Species/Organism) : Streptococcus equi subsp. Zooepidemicus
NCBI Protein GI : AAB92635
Taxonomy ID : 40041
Protein Name : M-like protein
Protein pI : 4.25
Protein Weight : 6257.51
Protein Length : 132
Protein Note : protectively immunogenic surface protein; hypervariable region

Protein Sequence : Show Sequence

>AAB92635.1 M-like protein, partial [Streptococcus equi subsp. zooepidemicus]
IPELEKKIQEVQEKLNYENRLSPERVGSDDEDDTEARRLIAEQEALKAELQK

Molecule Role : Protective antigen
Molecule Role Annotation : (Lin et al., 2011)

18. vicK

Gene Name : vicK
Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus MGCS10565
NCBI Gene ID : 6760966
NCBI Protein GI : 195978500
Locus Tag : Sez_1395
Genbank Accession : CP001129
Protein Accession : YP_002123744
Taxonomy ID : 552526
Gene Starting Position : 1433586
Gene Ending Position : 1434938
Gene Strand (Orientation) : -
Protein Name : TCS sensor kinase VicK
Protein pI : 5.4
Protein Weight : 49245.51
Protein Length : 450
Protein Note : similar to ref|YP_600051.1| Two-component sensor histidine kinase VicK [Streptococcus pyogenes MGAS2096]

DNA Sequence : Show Sequence

>gi|195977156:1433586-1434938 Streptococcus equi subsp. zooepidemicus MGCS10565, complete genome
ATTAATCAATATCATCCTCCCATTCATCATAGATAGCAGCATCCTTTTCATAAGGTAACACAATGGTAAA
GGTTGAGCCCTTGCCATAATCACTCTTAGCCCAAATAAAGCCTCGGTGCTGCTTAATAATTTCTTTAGCA
ATCGCTAGGCCTAGCCCGGTCCCTCCTTGAGCTCTACTCCTAGCCTTATCCACTCGGTAAAAACGGTCAA
AAATCAAAGGCAGGTCCTTTTTTGGAATACCCAAGCCCTCATCAGAAATCGAAATGATTAGCTGGGTATC
TGTTGTTTTCATGTTGACGACAATTTTTCCGCCATCGGGTGAATATTTGATCGCATTGTTTAAAATATTT
TCGATAACCTGCGTCATTTTGTCATCGTCAATTTCTAGCCAAATAGAGGTAATGGGGTAATCTCTGACAA
TTTCATAGGATTTTCCTGTGCTTGTGTGCTGATTTTTCACCAAATCAAAGCGGTTTAAGATAGAGGTCAT
AAAGGCTGTAAAGTTGGTCATTTCAACAGACAGCTGTGTCACATGATTATCAATTCTTGAGAGGTTGAGC
AAATCTGAAATCATGCGCATCATGCGATTGGTTTCATCTAGTGAGACCTTTATAAAGCTTGGAGCGATGT
CCTCCTTTAGCGCGCCCTCATCTAGAGCCTCTAGGTATGACTTGACAGAGGTCAAAGGTGTCCGCAGCTC
ATGGCTAACATTTGAAACAAAGAGTCTGCGCTCGCGCTCTTCCTTTTCCTGCTCAGTCGTGTCATGAAGT
ACAACAACCAGACCTGAAATAAAGCCGCTCTCTCGCCTATTAAGCGCAAAGCGCAGCCGCAGGGAAACAA
ATTCTCCTGTATCATTACGACGGTTTAAGGTCACAATCGGTGTTTTTGAGACCAGCTCTCGGTAGGTATA
AGGGGAGTCACTGTCTAATAAATCTGTAATGTTTTTTTCCAAGGCCTGCTCGCGATTTAAATTGAGCTGC
TTTTGAGCAGTCTCATTAATCATGATAATCTTGCCGGAACGATTGGTCGCAAGGACCCCATCTGTCATAT
AAGCCAAAATACTAGCAAGACGATTTTTTTCTTGAGCAAGGTTTTCATGAGTCAGACGAAAAACATCTGA
TAGGTCATTGAGCTGCTCAGACAGCTCCATCAATTCAATATCAGCCTTTTCATTGATCGTATCAGTATAG
CGACCATTGATCAAATCTCTTATCTTGTGGCTCATCAGACGAATGATCCGAGCATTGCGATAATCACGAA
TGGCTAAATGAATGAAATAAAAAGCAACAAAAACAAGCAATAAGAGTATGGCTAGCTCAAATGCAGAAAG
GTTTCCAATGATGTCCTTAGTCA

Protein Sequence : Show Sequence

>gi|195978500|ref|YP_002123744.1| TCS sensor kinase VicK [Streptococcus equi subsp. zooepidemicus MGCS10565]
MTKDIIGNLSAFELAILLLLVFVAFYFIHLAIRDYRNARIIRLMSHKIRDLINGRYTDTINEKADIELME
LSEQLNDLSDVFRLTHENLAQEKNRLASILAYMTDGVLATNRSGKIIMINETAQKQLNLNREQALEKNIT
DLLDSDSPYTYRELVSKTPIVTLNRRNDTGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERR
LFVSNVSHELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNHVTQLSV
EMTNFTAFMTSILNRFDLVKNQHTSTGKSYEIVRDYPITSIWLEIDDDKMTQVIENILNNAIKYSPDGGK
IVVNMKTTDTQLIISISDEGLGIPKKDLPLIFDRFYRVDKARSRAQGGTGLGLAIAKEIIKQHRGFIWAK
SDYGKGSTFTIVLPYEKDAAIYDEWEDDID

Molecule Role : Virmugen
Molecule Role Annotation : A vicK mutant is attenuated in mice and induced protection from challenge with wild type S. equi (Liu et al., 2008).
Related Vaccine(s): Streptococcus equi vicK mutant vaccine

III. Vaccine Related Host Genes

1. IgA

Gene Name : IgA
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 238447
Genbank Accession : AC160982
Taxonomy ID : 10090
Chromosome No : 12
Gene Starting Position : 8607
Gene Ending Position : 12639
Gene Strand (Orientation) : -
Protein Name : immunoglobulin heavy constant alpha
Protein Note : Also known as IgA; Igh-2

DNA Sequence : Show Sequence

>gi|121699722:8607-12639 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
AGTACTGGGGGACCTCTTTGCTGCCAAACGGGCCTCGAACAGTTGTAACAGTGAGTGCTGTGCTGTAGAA
CAAGCTCAGTAGGAAGAGGGTGAGGAAGGTCACAGTGGTGGGCCACAGGCTGGCACCTGGGGCCTCTTCC
TCCAGGATGTCTTCTGACTGGTCCAGTAGCACATAGGAAAGTGGCTCTTGACGTTCTGTAAGACATAGAT
GACCTTCTAGATCCAAACCCATGGGGATGGGGGCTCAGACTTAAGGTTTCTGCTTCTATGCCTCCTTGGG
CTTTGGGGACAGTGGTGGTAGGGGTCCCTTATTCCCGGGAAGGGACTTGTTCAGGGATAATGAGGAGGCA
GGGTCCCAGGTAAGGGACTGGAATCCCAGCAGTTCTTGAGATGGCCCCAAGACCCATCTCTCTCTCTTAG
GGAAACCTTAATTAGAGACCTGTCATGGTAAGAGAGTTGTTTCTGTCAAACTCTGAGAAGAGTTTGAACC
CAAGGCTCCTTAGGTCTCAGTCCTTTCTAGATATTTAGCAATACCTCCCTAAACCCCAGTTCTTAGGCTA
ATCTTGGCTTGGGTACTAGGGTTGTGTAAGATCAGACACCTTCAAACAGATACAGGCCAATGTGATTTCT
GGGCCAAAGTTAGACTACCAAGGCCTGGACATGTGGTCAGAGGACTCTGTTGCATTCTTAAGAGCTGTGA
CTGGGGGTTAAGCAACAGTAAAGACCCCCAGTAGAGCCAACCCTGCTGTCCTCAGGTTCTCTGCGCACAG
AGTTTCATTTTAAGTGGCAGTAGGAAATGTGTACTGATAGTCAACCACTCGTATTTCTGTCACTGCAGCC
ATCCATCAATCCATCCATCATCCCCATCGTTCATCCTTGTAGCCAGCCATCCATTACCCTCCAGCCGCTC
ACTCATCATCTTTCCCGACTATCGTGATTCTCCAGCTTCTCTGCCCCCTCTCCCTGTGGGGATTCAGTCT
TTCACCTATTGACTCCTCCTTACTAGGATTCTCTGTCATGGGCCAGGCCTTGGATTCCATGGGTGGCTAT
GGTACTTTTCTGAGTCAGGTTTCCCAAGCTGGTCTGTTGCTCTTGCTATTCTCAGGGAACTGGACTTTCA
AGCTGGAGAGTATCCAGGGCATGCATGGGCCTTTACTCCACTCTTGCTCTGAGAGCAGAGGGAAGTTGCT
AGAAACCAGCAACTCCTCCCCCCCCCCCCCGAAGTTCTGAAGGCCTGTGAGACAGGAGCAGGGCCCCCAC
TGCCCTCTTGTGGACACCTGGGCTCCAAGTATACCTGGTTGTAGAACTTTGTGTTCTAGGGGTGCCCATG
GAGGAGGCCGAGGCCTGTGTGGGAGTGGTCTCCATGGCCTGCTCCTGCTTCTTCCCAGATTTGCCTCTTA
TCCTGCTTCTGGTTTGTGATACTTGGGCTCAGGATGGGAAGGGAGACTTAGGTTAGAAAGGGGGCACCTG
GAGGGGCCTATGCCCTTGTCCATTGTGTGTAGTTGTCAGTGTCGGGCCTGGAAATTCTCCCGGGGGCTTG
GAGGGGACTCCCCTTGCTTTCCAGAAGCTGGGTGTGGGCTGTGAACTTCATCTTCCTCCAGGGTTCAGGT
CTGTGTGAGTGTGCAAATGTGAGTGTCTGTGCATGTATGCCCATGTGCATGTATGCAGAACAGGGTGTAT
GCCCATGTGCATGTGTGAAGAGCAGGGTGCTGCTGAGGGTTGTCTGTGCCATGCTATATGTCTTTCTCAT
GAGTCAGTGGTCACCCTGAATGCATGTTTCCATTCAGGGATGGCCTACTGAGGGTGCGTGGCATCTTCTT
CCCAGTGCCCCTGTGTTCATCCCTTCATTCTCATACCATCATCTCTATTGTGACCCCCACACGGCACCAG
CCTTGATGGTGCTACCCTTTTGCTCCATCTATCCCTGTCTTGTTTGGGTTAGAAGCTGCGGTCTAATAAA
CAGCCACACCACCCACCACTCAGAGTACTTGTGCAGCAACCCACTGCCCTGGGGACTCTCCCATGAGCTT
TGCCTTCTTGGCTGAGTCTGCTCGCCTTGGATTTCATCCCCAGTTTATTCTTACTTCCCATTCACTCATT
GCACATTCACAGGGCAGGTGCACACACTCTCATGTTTCCACCAACATGAACACACATAGATATATGCATG
CTCATAGGTATGCACAGGCACACACTTGCTCACAGGGACATAGATATAGTGTGTCCACATATGTACTTAT
ACCCATATACATACCTATAAACCATAGGTGCACACAAACTCACTCCTCTCTTGCTGCACACAGGTACACA
CATGAAGGCACATGGACCCATCCATAAGCACTCACCAATACATAGACATGCATACACATATACTCTAGGC
ATGAAGGGTCTTCAAAGTCTCCGAGGCCTTACAAGCTTAGGTGGTGGTGTTTCTCCCTCCCTGGGCCCTG
ACCCCTCCCTGTGTTCTGTAGACCCTAAGGATGATGGACAGGCACTGGATGGAAGTGCAGGGATACTTTG
GATGAGCACAGAGTTTATTTCAGGAGTAGGGACAGGCAGGGTGGCTCAGTAGCAGATGCCATCTCCCTCT
GACATGATCACAGACACGCTGACATTGGTGGGTTTACCCGACAGACGGTCGATGGTCTTCTGGGTGAAGT
TCATGGGCAAGGCCTCGTGGCCCACCATGCAGGAGTACTGGTCACCCTGTTTCCAGAGTTCAGCTGATAC
ACGCAACACGCTTGTCACCAGGTAGGTGGTGGCTCCCTCGCCTGGCTCCTTTAGGGGCTCAAACACTAGG
TAGCTTTCTGGGGACAGCTCCTCATTTCCATGCAGCCATCGCACCAGCACTTCTTTAGGGTTGAAAGCTC
GCACCAGGCATGTCAGGGACACGAGCTCATTCAGGGCCAGCTCCTCCGACGGCGGCGGTAGCAGGTGGAC
CTGGGGTGGGAAGGTGTTCACTGGAAGGTAGAAAGAAGAGTTATGGCTCAGACAAGGAGAGCAAGACCCC
TCTGCCCTTCCCTTCTGGGCAGTTCCTCAGATAGCTCCAATTTCCCTCTGTGATATAAGGACAGGAGCAG
TTAGTTGAGCATCTGTATTATAGGAAGAAAGCAGGCATGTGAGCAGGGAACGTCATACAATGTCCCGGGT
ATGCATCTGGGCTCACCTGTGATTTTGGCAATTGTGCCAGTTAAGGTGTCAGACTCAGGATGGGTAACTG
TGCACTTGAATGATGCGCCACTGTTCCAGCGCTCAGCACAGCCAGGCAGGACGCTGGACACACTGTAGCA
GCCGCAGGAATTCTGCACAGCTTTCTTCTGCACTGCATCCTTCCCAGTGGAGGGCTCCCAGGTGAAGACA
GCTCCCTCAGGATTTCTCAGGCCATTCAGAGTACATGTGAGGCTGGCATCTGAACCCAGGAGCAGGTCCT
CAAGAGCTGGCCGCTGCAGTGACAGGCTGGGATGGCAGGAAGGAGGACAAGGAGGACAAGGAGGAGGAGG
ACCTGTAACAGAGAAGTCCTAGCAAATCAGTATATTCTCCTTTTCTCCTTTGCTGTCCCCTTTTCTGGAT
GTTCTGAGGCCTCAGTTCCTTATGGCCTGTTTACGCTTCCCTTCCCCCACCCCCATGGGAGGTTTCTGAG
GGTCCCTGTGAGGGTGTGCCTCAGAGGGATATATGGATCTAGATAAGGTAGAACTTATCCCACCCCCAGC
TGACCCCCTAACGTTCTTTACCAGAGCACTTCACATCCAATTCTTGGACGGCGTTAGAGTCATGTTGCAC
GGAACATTTCACGGATTCTCCTTCTGGGCACTCGACAGCTGGCAGGGTCAACTGGCTGCTCATGGTGTAC
CCTCCCCCAGAGGCCAGGGCAGGTGGGAAGTTTACGGTGGTTATATCCTTCCCACTCTTTCCCCAGGTCA
CATTCATCGTGCCGGAAGGGAAGTAATCGTGAATCAGGCAGCCGATTATCACTGGGTCACTTGACAGAGC
TCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGAC

Molecule Role : Vaximmutor
Related Vaccine(s): S. equi Subunit EAG, FNZ and SFS Protein Vaccine , Streptococcus equi vicK mutant vaccine

2. IgG

Gene Name : IgG
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16059
Genbank Accession : AF010213
Taxonomy ID : 10090
Chromosome No : 12
Gene Starting Position : 881411
Gene Ending Position : 914690
Gene Strand (Orientation) : +
Protein Name : immunoglobulin heavy chain (V7183 family)
Protein Note : Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)

DNA Sequence : Show Sequence

>gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
ACAAGCACAGTGCAAATTTCTGTCACCCTG

Molecule Role : Vaximmutor
Additional Molecule Role : Vaximmutor
Related Vaccine(s): Streptococcus equi vicK mutant vaccine

IV. Vaccine Information

1. Equilis StrepE

a. Tradename:

Equilis StrepE

b. Manufacturer:

Intervet

c. Vaccine Ontology ID:

VO_0001054

d. Type:

Live, attenuated vaccine

e. Status:

Licensed

f. Host Species for Licensed Use:

Horse

g. Immunization Route

Intramuscular injection (i.m.)

h. Description

Live submucosal vaccine; deletions in aroA gene(Jacobs et al., 2000)

2. S. equi Subunit CNE Protein Vaccine

a. Vaccine Ontology ID:

VO_0011546

b. Type:

Subunit vaccine

c. Status:

Research

d. Gene Engineering of collagen binding, ancillary pilus subunit Cne

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

e. Adjuvant:

VO ID: VO_0001324

f. Immunization Route

Intranasally

g. Mouse Response

Host Strain: NMRI mice
Vaccination Protocol: Fifteen to twenty microgram of each antigen and 10 μg of the adjuvant in a total volume of 12–16 μl were given to the mice by placing the antigens onto the nares and allowing the mice to inhale. This was done at three occasions at 1-week intervals. In each experiment, control animals were given only adjuvant at the same concentration omitting the antigen (Flock et al., 2006).
Challenge Protocol: A dose containing 10^6 CFU in a 10-μl volume was used for infection of mice (Flock et al., 2006).
Efficacy: Mice immunized with recombinant CNE lost less weight and had less nasal colonization than the unvaccinated controls (Flock et al., 2006).

3. S. equi Subunit EAG, FNZ and SFS Protein Vaccine

a. Vaccine Ontology ID:

VO_0011544

b. Type:

Subunit vaccine

c. Status:

Research

d. Gene Engineering of binding protein Eag

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

e. Gene Engineering of Fnz

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Gene Engineering of Sfs

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

g. Adjuvant:

VO ID: VO_0001272
Description: The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin (Flock et al., 2004).

h. Immunization Route

Subcutaneously

i. Mouse Response

Vaccination Protocol: Mice were immunized either subcutaneously (s.c.) or intranasally (i.n.). For each antigen (FNZ, SFS, and EAG), 12 μg of antigen was mixed with an equal amount of EtxB so that all components achieved a final concentration of 300 μg/ml. In the case of i.n. immunization, the total volume was kept to less than 40 μl and applied into the nostrils of anaesthetized mice . For both s.c. and i.n. immunizations, four doses were given with 1-week intervals (Flock et al., 2004).
Challenge Protocol: Challenge i.n. with S. equi subsp. equi (10^6 CFU in a 10-μl volume) was given 1 week after the last booster dose (Flock et al., 2004).
Efficacy: It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) (Flock et al., 2004).
Host Gene Response of IgA
- Gene Response: An analysis of the bronchoalveolar lavage (BAL) fluid taken after the last immunization revealed significant IgA levels to EAG in the animals immunized with the three antigens and the EtxB adjuvant. By contrast, mice immunized with the three S. equi antigens in the absence of EtxB gave significantly lower IgA responses (Flock et al., 2004).
- Detailed Gene Information: Click here.

4. Streptococcus Equi Live Culture Vaccine (USDA: 1831.00)

a. Manufacturer:

Wyeth

b. Vaccine Ontology ID:

VO_0001784

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Horse

5. Streptococcus equi vicK mutant vaccine

a. Vaccine Ontology ID:

VO_0002798

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of vicK

Type: Gene mutation
Description: This vicK mutant is from Streptococcus equi (Liu et al., 2008).
Detailed Gene Information: Click here.

f. Immunization Route

intranasal immunization

g. Mouse Response

Persistence: A vicK mutant is attenuated in mice (Liu et al., 2008).
Efficacy: A vicK mutant induces protection in mice from challenge with wild type S. equi (Liu et al., 2008).

V. References

1. Chanter et al., 1999: Chanter N, Ward CL, Talbot NC, Flanagan JA, Binns M, Houghton SB, Smith KC, Mumford JA. Recombinant hyaluronate associated protein as a protective immunogen against Streptococcus equi and Streptococcus zooepidemicus challenge in mice. Microbial pathogenesis. 1999; 27(3); 133-143. [PubMed: 10455004].

2. Flock et al., 2004: Flock M, Jacobsson K, Frykberg L, Hirst TR, Franklin A, Guss B, Flock JI. Recombinant Streptococcus equi proteins protect mice in challenge experiments and induce immune response in horses. Infection and immunity. 2004; 72(6); 3228-3236. [PubMed: 15155624].

3. Flock et al., 2004: Flock M, Jacobsson K, Frykberg L, Hirst TR, Franklin A, Guss B, Flock JI. Recombinant Streptococcus equi proteins protect mice in challenge experiments and induce immune response in horses. Infection and immunity. 2004; 72(6); 3228-3236. [PubMed: 15155624].

4. Flock et al., 2006: Flock M, Karlström A, Lannergård J, Guss B, Flock JI. Protective effect of vaccination with recombinant proteins from Streptococcus equi subspecies equi in a strangles model in the mouse. Vaccine. 2006; 24(19); 4144-4151. [PubMed: 16580099].

5. Fu et al., 2013: Fu Q, Wei Z, Liu X, Xiao P, Lu Z, Chen Y. Glyceraldehyde-3-phosphate dehydrogenase, an immunogenic Streptococcus equi ssp. zooepidemicus adhesion protein and protective antigen. Journal of microbiology and biotechnology. 2013; 23(4); 579-585. [PubMed: 23568215].

6. Fu et al., 2013: Fu Q, Wei Z, Chen Y, Xiao P, Lu Z, Liu X. Identification of a surface protective antigen, CSP of Streptococcus equi ssp. zooepidemicus. Vaccine. 2013; 31(10); 1400-1405. [PubMed: 23306366].

7. Jacobs et al., 2000: Jacobs AA, Goovaerts D, Nuijten PJ, Theelen RP, Hartford OM, Foster TJ. Investigations towards an efficacious and safe strangles vaccine: submucosal vaccination with a live attenuated Streptococcus equi. The Veterinary record. 2000; 147(20); 563-567. [PubMed: 11104039].

8. Lin et al., 2011: Lin HX, Huang DY, Wang Y, Lu CP, Fan HJ. A novel vaccine against Streptococcus equi ssp. zooepidemicus infections: the recombinant swinepox virus expressing M-like protein. Vaccine. 2011; 29(40); 7027-7034. [PubMed: 21807055].

9. Liu et al., 2008: Liu M, McClure MJ, Zhu H, Xie G, Lei B. The Two-Component Regulatory System VicRK is Important to Virulence of Streptococcus equi Subspecies equi. The open microbiology journal. 2008; 2; 89-93. [PubMed: 19088917].

10. Meehan et al., 1998: Meehan M, Nowlan P, Owen P. Affinity purification and characterization of a fibrinogen-binding protein complex which protects mice against lethal challenge with Streptococcus equi subsp. equi. Microbiology (Reading, England). 1998; 144 ( Pt 4); 993-991003. [PubMed: 9579073].

11. MicrobeWiki: S. zooepidemicus: MicrobeWiki: S. zooepidemicus [http://microbewiki.kenyon.edu/index.php/Streptococcus_zooepidemicus]

12. Nally et al., 2000: Nally JE, Artiushin S, Sheoran AS, Burns PJ, Simon B, Gilley RM, Gibson J, Sullivan S, Timoney JF. Induction of mucosal and systemic antibody specific for SeMF3 of Streptococcus equi by intranasal vaccination using a sucrose acetate isobutyrate based delivery system. Vaccine. 2000; 19(4-5); 492-497. [PubMed: 11027813].

13. Timoney, 2004: Timoney JF. The pathogenic equine streptococci. Veterinary research. 2004; 35(4); 397-409. [PubMed: 15236673].

14. Velineni and Timoney, 2013: Velineni S, Timoney JF. Characterization and protective immunogenicity of the SzM protein of Streptococcus zooepidemicus NC78 from a clonal outbreak of equine respiratory disease. Clinical and vaccine immunology : CVI. 2013; 20(8); 1181-1188. [PubMed: 23740925].

15. Waller et al., 2007: Waller A, Flock M, Smith K, Robinson C, Mitchell Z, Karlstr�m A, Lannerg�rd J, Bergman R, Guss B, Flock JI. Vaccination of horses against strangles using recombinant antigens from Streptococcus equi. Vaccine. 2007; 25(18); 3629-3635. [PubMed: 17321016].

16. Wei et al., 2012: Wei Z, Fu Q, Liu X, Xiao P, Lu Z, Chen Y. Identification of Streptococcus equi ssp. zooepidemicus surface associated proteins by enzymatic shaving. Veterinary microbiology. 2012; 159(3-4); 519-525. [PubMed: 22613253].

17. Wei et al., 2013: Wei Z, Fu Q, Chen Y, Li M, Cong P, Mo D, Liu X. Streptococcus equi ssp. zooepidemicus C5a peptidase, a putative invasin, induces protective immune response in mice. Research in veterinary science. 2013; 95(2); 444-450. [PubMed: 23632199].

18. Wiki: Strangles: Strangles [http://en.wikipedia.org/wiki/Streptococcus_equi]