VIOLIN: Vaccine Investigation and Online Information Network

Pseudorabies virus

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. gB (Protective antigen)
2. GI (Protective antigen)
3. GII (Protective antigen)
4. GIII (Protective antigen)
5. gp50 (Protective antigen)
6. IE180 (Protective antigen)
7. UL44 (Protective antigen)
8. US6 (Protective antigen)
9. glycoprotein E (Virmugen)
10. glycoprotein G (Virmugen)
11. glycoprotein M (Virmugen)
12. gp50 (Virmugen)
13. gp63 (Virmugen)
14. UL23 (Virmugen)
15. UL50 (Virmugen)
16. US3 (Virmugen)
Vaccine Related Host Genes
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

10345

2. Disease:

Aujeszky's disease

3. Introduction

Pseudorabies is a viral disease in swine that is endemic in most parts of the world. It is caused by porcine herpesvirus 1, which is also called pseudorabies virus (PRV) or suid herpesvirus-1 (SHV-1) and is also known as Aujeszky's disease, and in cattle as mad itch. PRV is considered to be the most economically important viral disease of swine in areas where hog cholera has been eradicated. Other domestic and wild mammals, such a cattle, sheep, dogs, raccoons are also susceptible. The disease is usually fatal in these hosts. The virus is shed in the saliva and nasal secretions of infected swine and is spread through oral or nasal contact. Aerosolization of the virus and transmission by fomites also may occur. The virus may potentially survive for seven hours in humid air and spread up to two kilometers. Furthermore, it may survive on well water for up to seven hours, in green grass, soil, and feces for up to two days, in contaminated feed for up to three days, and in straw bedding for up to four days (Wiki: Pseudorabies).

4. Microbial Pathogenesis

After natural infection, the primary site of viral replication is nasal, pharyngeal, or tonsillar epithelium. The virus spreads via the lymphatics to regional lymph nodes, where replication continues. Virus also spreads via nervous tissue to the brain, where it replicates, preferentially in neurons of the pons and medulla. In addition, virus has been isolated from alveolar macrophages, bronchial epithelium, spleen, lymph nodes, trophoblasts, embryos, and luteal cells (Merck Vet Manual: Pseudorabies).

5. Host Ranges and Animal Models

Although the pig is the only natural host, the virus can infect cattle, sheep, cats, dogs, and goats as well as wildlife, including raccoons, opossums, skunks, and rodents. Experimental studies in nonhuman primates indicate that rhesus monkeys and marmosets are susceptible but chimpanzees are not. Reports of human infection are limited and are based on seroconversion rather than virus isolation. Infections in horses are rare (Merck Vet Manual: Pseudorabies).

II. Vaccine Related Pathogen Genes

1. gB

Gene Name : gB
Sequence Strain (Species/Organism) : Suid herpesvirus 1
VO ID : VO_0011238
NCBI Protein GI : 4138504
Other Database IDs : UniProtKB/TrEMBL: Q9WJ33
Taxonomy ID : 10345
Gene Starting Position : 16807
Gene Ending Position : 19721
Gene Strand (Orientation) : -
Protein Name : envelope glycoprotein B
Protein pI : 3.29
Protein Weight : 1207.11
Protein Length : 60
Protein Note : strains Kaplan (Ka), Becker (Be), Rice, Indiana-Funkhauser (In-Fh), NIA-33 and TNL

Protein Sequence : Show Sequence

>CAA09075.1 gB, partial [Suid alphaherpesvirus 1]
ESEDPDAL

Molecule Role : Protective antigen
Molecule Role Annotation : Recombinant adenovirus expressing gB (rAd-gB) was found to induce effective protective immunity against a virulent viral infection, regardless of whether it was administered via the muscular or systemic route (Han et al., 2008).
Related Vaccine(s): Pseudorabies Subunit gB Protein Vaccine

2. GI

Gene Name : GI
Sequence Strain (Species/Organism) : Suid herpesvirus 1
VO ID : VO_0011251
NCBI Protein GI : 255957222
Other Database IDs : CDD:177577
CDD:165547
Taxonomy ID : 10345
Gene Strand (Orientation) : ?
Protein Name : envelope glycoprotein gI
Protein pI : 9.1
Protein Weight : 35302.37
Protein Length : 440
Protein Note : envelope glycoprotein I; Provisional

Protein Sequence : Show Sequence

>ACU43467.1 envelope glycoprotein gI, partial [Suid alphaherpesvirus 1]
RDVTRLPAGLLLAALTLAALTPRVGGVLFRGAGVSVHVAGSAVLVPGDAPNLTIDGTLLFLEGPSPSNYS
GRVELLRLDPKRACYTREYAAEYDLCPRVHHEAFRGCLRKREPLARRASAAVEARRLLFVSRPAPPDAGS
YVLRVRVNGTTDLFVLTALVPPRGRPHHPTPSSADECRPVVGSWHDSLRAVDPAEDAVFTTQPPPEPEPP
TTPAPPRGTGATPEPRSDEEEEDEEGATTAMTPAPGTLDANGTMVLNASVVSRVLLAAANATAGARGPGK
IAMVLGPTIVVLLIFLGGVACAARRCARNRIYRPRPGRGPAVHAPPPRRPPPSPVAGAPVPQPKMTLAEL
RQKLATIAEEQ

Molecule Role : Protective antigen
Molecule Role Annotation : Vaccination of mice with fusion proteins containing the N terminus of gI from Suid herpesvirus 1 are able to confer protection to mice against a lethal challenge of PRV (Fuchs et al., 1990).
Related Vaccine(s): Pseudorabies Subunit gI Protein Vaccine

3. GII

Gene Name : GII
Sequence Strain (Species/Organism) : Suid herpesvirus 1
VO ID : VO_0011249
NCBI Protein GI : 1335775
Other Database IDs : CDD:223014
Taxonomy ID : 10345
Gene Strand (Orientation) : ?
Protein Name : glycoprotein gII
Protein pI : 11.38
Protein Weight : 14546.78
Protein Length : 200
Protein Note : glycoprotein BALF4; Provisional

Protein Sequence : Show Sequence

>AAB26946.1 glycoprotein gII, partial [Suid alphaherpesvirus 1]
PFGALAIGLLVLAGLVAAFLAYRHISRLRRNPMKALYPVTTKALKEDGVEEGDVDEAKLDQARDMIRYMS
IVSALEQQEHRRARRTAGPRSWPAGSGRWLRAAGTTSASRARTPTPCSPLPRETIKMRLFGNT

Molecule Role : Protective antigen
Molecule Role Annotation : A NYVAC vaccinia vector containing genes for pseudorables virus glycoproteins gII and gp50 was administered to pigs to determine if it would have a greater protective effect than a vector containing the gene for gp50 alone. Both NYVAC vectors protected pigs similarly from virulent pseudorabies virus challenge (Brockmeier and Mengeling, 1996).
Related Vaccine(s): NYVAC-PRV- gII/ gp50 , Pseudorabies recombinant vector vaccine encoding gp50 and gII protein

4. GIII

Gene Name : GIII
Sequence Strain (Species/Organism) : Suid herpesvirus 1
VO ID : VO_0011250
NCBI Protein GI : 334050
Other Database IDs : CDD:223030
CDD:280317
Taxonomy ID : 10345
Gene Strand (Orientation) : ?
Protein Name : glycoprotein gIII mature peptide
Protein pI : 6.78
Protein Weight : 46490.04
Protein Length : 543
Protein Note : glycoprotein gIII signal peptide

Protein Sequence : Show Sequence

>AAA47464.1 glycoprotein gIII [Suid alphaherpesvirus 1]
MASLARAMLALLALYAAAIAAAPSTTTALDTTPNGGGGGNSSEGELSPSPPPTPAPASPEAGAVSTPPVP
PPSVSRRKPPRNNNRTRVHGDKATAHGRKRIVCRERLFSARVGDAVSFGCAVFPRAGETFEVRFYRRGRF
RSPDADPEYFDEPPRPELPRERLLFSSANASLAHADALAPVVVEGERATVANVSGEVSVRVAAADAETEG
VYTWRVLSANGTEVRSANVSLLLYSQPEFGLSAPPVLFGEPFRAVCVVRDYYPRRSVRLRWFADEHPVDA
AFVTNSTVADELGRRTRVSVVNVTRADVPGLAAADAADALAPSLRCEAVWYRDSVASQRFSEALRPHVYH
PAAVSVRFVEGFAVCDGLCVPPEARLAWSDHAADTVYHLGACAEHPGLLNVRSARPLSDLDGPVDYTCRL
EGLPSQLPVFEDTQRYDASPASVSWPVVSSMIVVIAGIGILAIVLVIMATCVYYRQAGP

Molecule Role : Protective antigen
Molecule Role Annotation : Mice immunized with either gII, gIII, gVI antigen or a mixture of them were challenged intraperitoneally with 8.5 x 10(3) plaque forming units of PRV. All the mice immunized with 1.5 and 4.5 micrograms of the mixture and 4.5 micrograms of the gIII antigen survived (Matsuda et al., 1992).

5. glycoprotein E

Gene Name : glycoprotein E
Sequence Strain (Species/Organism) : Suid herpesvirus 1
NCBI Protein GI : 307634504
Other Database IDs : CDD:177575
CDD:190321
Taxonomy ID : 10345
Gene Strand (Orientation) : ?
Protein Name : glycoprotein E
Protein Length : 578
Protein Note : envelope glycoprotein E; Provisional

Protein Sequence : Show Sequence

>gi|307634504|gb|ADN78283.1| glycoprotein E [Suid herpesvirus 1]
MRPFLLRAAQLLALLALALSTEAPSLSAETTPGPVTEVPSPSVEVWDDLSTEAGDDDLNGDLDGDDRRAG
FGSALASLREAPPAHLVNVSEGANFTLDARGDGAVLAGIWTFLPVRGCDAVSVTTACFETACHPDLVLGR
ACVPEAPEMGIGDYLPPEVPRLRREPPIVTPERWSPHLSVLRATPNDTGLYTLHDASGPRAVFFVAVGDR
PPAPADPVGPARHEPRFHALGFHSQLFSPGDTFDLMPRVVSDMGDSRENFTATLDWYYARAPPRCLLYYA
YEPCIYHPRAPECLRPVDPACSFTSPARARLVARRAYASCSPLLGDRWLTACPFDAFGEEVHTNATADES
GLYVLVMTHNGHVATWDYTLVATAAEYVTVIKELTAPARAPGTPWGPGGGDDPIYVDGVTTPAPPARPWN
PYGRTTPGRLFVLALGSFVMTCVVGGAVWLCVLCSRRRAASRPFRVPTRARTHMLSPVYTSLPTHEDYYD
GDDDDEEAGVIRRRPASPGGDSGYEGSYASLDPEDEFSSDEDNGLYVRPEEAPRSGFDVWFRDPEKPEVT
NGPNYGVTANRLLNARPA

Molecule Role : Virmugen
Molecule Role Annotation : A glycoprotein E mutant, in combination with UL23 and glycoprotein G mutations, is attenuated in raccoons, with a 75% survival rate. This mutant also provides some protection from wild type PRV (Weigel et al., 2003).
Related Vaccine(s): Pseudorabies virus glycoprotein E/G/UL23 mutant vaccine , rORFV-PRV-gC/gD

6. glycoprotein G

Gene Name : glycoprotein G
Sequence Strain (Species/Organism) : Suid herpesvirus 1
NCBI Protein GI : 15422177
Other Database IDs : CDD:186820
Taxonomy ID : 10345
Gene Strand (Orientation) : ?
Protein Name : glycoprotein G
Protein Length : 499
Protein Note : Herpesvirus glycoprotein D/GG/GX domain; cl08328

Protein Sequence : Show Sequence

>gi|15422177|gb|AAK95859.1| glycoprotein G [Suid herpesvirus 1]
MKWATWILALGLLVVRTVVAREAPRELCYGHPVHDDRRPVGPATDAQPVNPLAPANATGTDYSRGCEMRL
LDPPLDVSSRSSDPVNVTVAWFFDGGHCKVPLVHREYYGCPGDAMPSVETCTGGYSYTRTRIDTLMEYAL
VNASLVLQPGLYDAGLYIVVLVFGDDAYLGTVSLSVEANLDYPCGMKHGLTITRPGATLPPIAPTAGDHQ
RWRGCFPSTDEGAWENVTAAEKGLSDDYADYYDVHIFRLESDDEVVHGDAPEAPEGEEVTEEEAELTSSD
LDNIEIEVVGSPAAPVEGAGDGEEGHGDEEDEELTSSDLDNIEIEVVGSPAAARFFAASTTPRAPTRAAE
ITTMTTVTTVRTTEDPSGITDCRRSDFVSPSDIFVTPTGSPALLLGFLGSALASRPLHLTAGETAQHVRE
AQQKSRHVRSLGGLQLSVETETTNTTTTQTGLSGDIRTSIYICVALAGLVVVGIVIMCLHMAIIRARARN
DGYRHVASA

Molecule Role : Virmugen
Molecule Role Annotation : A glycoprotein G mutant, in combination with UL23 and glycoprotein E mutations, is attenuated in raccoons, with a 75% survival rate. This mutant also provides some protection from wild type PRV (Weigel et al., 2003).
Related Vaccine(s): Pseudorabies virus glycoprotein E/G/UL23 mutant vaccine

7. glycoprotein M

Gene Name : glycoprotein M
NCBI Protein GI : 1478255
Other Database IDs : CDD:177566
GOA:Q85041
InterPro: IPR000785
UniProtKB/UniProt: Q85041
Taxonomy ID : 33703
Gene Strand (Orientation) : ?
Protein Name : UL10
Protein Length : 525
Protein Note : envelope glycoprotein M; Provisional

Protein Sequence : Show Sequence

>gi|1478255|emb|CAA65905.1| UL10 [Suid herpesvirus 1 strain Kaplan]
MTYASESKYVTNPRPASSIRLARVRVKLRRQDRTSALISVAASAAESHFSSAVVLPEPIGARATRTRRQA
VSCGDGSSTGASRCRSRETPGQRLRTTAGRSQPYSIRASDTLEANAPPSSSAPPRRPPASAAMCGPRNAE
AVSWRSWLIEVCGFALAALTLVLTLIFASLPEMGFPCFYATVADYDTLNDTSGGVWTRQPLVAPALFLET
PTVTSFFGFTATVLLAHALYAVAGAVVLRREAGRLAFQPSVVLYAASTVAAPGTLMLGALCAWTLQAVVL
LMAHKQAGLAAAAYITHFVFLALFGACHACKGTGDVRAALAASPPLRRVAVHARAVVTNVVLGAVGLGAA
VVGLMLGVLLANSFHISLWKTAEAALAVFTLLALALMVFVEVVVSGYVQVLPTPAFCVLVASAAFGVSAH
RYFAKFSEALGETHGVVIGTRAVLAVLSLIALAMIVVRLVRACIAHRARGSRFYANVDKARTTARRYLQK
RLHGRGNDEYLLAPGSGDDEFDDGDEVVYENLGFE

Molecule Role : Virmugen
Molecule Role Annotation : A glycoprotein M, gM, deletion in pseudorabies virus was attenuated and induced protection in piglets against intranasal challenge with 10⁸ p.f.u. of the highly virulent PrV strain NIA-3 ((Dijkstra et al., 1997).
Related Vaccine(s): Psuedorabies virus glycoprotein M mutant vaccine

8. gp50

Gene Name : gp50
Sequence Strain (Species/Organism) : Suid herpesvirus 1
VO ID : VO_0011248
NCBI Protein GI : 2440155
Other Database IDs : CDD:279830
InterPro: IPR002896
UniProtKB/TrEMBL: O41201
Taxonomy ID : 10345
Gene Strand (Orientation) : ?
Protein Name : glycoprotein gp50
Protein pI : 5.27
Protein Weight : 25087.4
Protein Length : 307
Protein Note : isolated from camels

Protein Sequence : Show Sequence

>CAA75105.1 glycoprotein gp50, partial [Suid alphaherpesvirus 1]
MLLAALLAALVARTTLGADVDAVPAPTLPPPAYPYTESWQLTLTTVPSPFVGPADVYHTRPPEDPCGVVA
LISDPQVDRLLNEAVAHRRPTYRAHVAWYRIADGCAHLLYFIEYADCDPRQIFGRCRRRTTPMWWTPSAD
YMFPTEDELGLLMVAPGRFNEGQYRRLVSVDGVNILTDFMVALPEGQECPFARVDQHRTYKFGACWSDDS
FNGGVDVMRFLTPFYQQPPHREVVNYW

Molecule Role : Protective antigen
Molecule Role Annotation : A NYVAC vaccinia vector containing genes for pseudorables virus glycoproteins gII and gp50 was administered to pigs to determine if it would have a greater protective effect than a vector containing the gene for gp50 alone. Both NYVAC vectors protected pigs similarly from virulent pseudorabies virus challenge (Brockmeier and Mengeling, 1996).
Related Vaccine(s): Pseudorabies recombinant vector vaccine encoding gp50 and gII protein , Pseudorabies recombinant vector vaccine encoding gp50 protein

9. gp50

Gene Name : gp50
Sequence Strain (Species/Organism) : Suid herpesvirus 1
NCBI Gene ID : 2952548
NCBI Protein GI : 51557533
Locus Tag : SuHV1_gp50
Genbank Accession : BK001744
Protein Accession : YP_068367
Taxonomy ID : 10345
Gene Starting Position : 83052
Gene Ending Position : 86540
Gene Strand (Orientation) : +
Protein pI : 9.08
Protein Weight : 46944.96
Protein Length : 470

DNA Sequence : Show Sequence

>gi|51557483:83052-86540 Suid herpesvirus 1, complete genome
CATGTTCGCCTACGTGAAGCCGATGGCGCACGGCCCGGACATGGCCTCCGTCTACCAGTCCACGGGGCGC
GTGCGGCGGCTGCTGCGCGACGAGCTCTTCGTGTACGTGGACGGCTCCGGCGCGCGCGGCGAGCCCATCT
TCACGCCCGTGCTGCTGAACCACGTCGTGGGCTCGGGCTGGCCGGCGCGCCTGTCGCAGGTGACGAACCT
CGTGTGCGCGCAGTTCCAGCGCCGCTGCCGCCCGGCGTTCGCGGCGGCGCGCGGGATGCGCCTCTTCTCC
CGCTTCAAGTTCAAGCACCTGTTCGAGCGCTGCACGCTGACGAGCGTCAACGACAGCCTCAACATCCTGC
ACGCGCTGCTGGAGAACAACCGCCTGCGGGTGGCGCTCGAGGGCTGCGAGCCGCCGCTGACGGCGCGCGC
CTTCTGCGACTTTCTGCGCGACGCGCGCCTGGACGCCTTCGCCAGCCAGCAGGTGCTGCGGCAGCTGCGC
CCCCCGGACCGGCCGGTGGCGGCCGACATCGCGGACAGCGGCGAGGTGGCCACCTTCGTGGAGAAGTACC
TCGTGGCCGACGTGCCCGAGGACGAGCTGCAGGAGCTGCTGCGCGCGCTCGCCAACCCCGTGACGCGGGA
GCAGTTTGTGGGCCTGGCCGTGCTCGGCGCGTGCGCGCGCGTCCCCGAGGCGCTGCGCAGCGAGCGCGTC
TTCGGGGCCGTGTACGGGCACTACGCCTCCGGGGCCGTGCCCGTGGTGGCCGACGGGCGGCTGGAGCTGG
CGGCGCTGGCCCCCGACTTCAACGTGCCCGCGCGCTGGGCCCTCACGCGGCGCTGCGCGCGCGTCGCGGA
GGCCGCCGGGCTCTTCGAGGGCGCCTCGCCGGAGGTGGACTCGGCCGCGGTCGCGGCGGCGGCGGCCGAC
GCCGAGCTGGCGCCGCTGCTGCTCGAGGTGCTGCGGTGCCACGTGCTGGACGCGACGACGGCCGCGCGGC
GCCCCGTGCGCGCGGCCCTGAGCGCGCTGGGGGCGGGCGGGGGCGCGGGCCCGCTGAGCCGCGGGCGCCA
CGCCGCGCTCGTGTTCAAGGTGATGTGGGAGGAGGCCTTCGGCGTGCGCGTCGGCCGCAGCCGGCAGACG
TTCCCGGGGCCCACGCGCGTGAAGAACCTGCGCAAGGCCGAGATCGCCGCGCTGCTGCGCGACGCCGGCC
TGGACCCGCCCGCGGGCGCCACCCACCGGCAGCTGTACGCCCTGCTGATGGAACGCCGGGGGGACTTTGC
GGGGGAGAGGTATAAACTGCGCCTGCCCGCCTGGAGCCGGCTCATGTACCTGACCCAGGGCGGCTTCGAC
GCGCCGCTGGACGCCGCGCTCTCGCTCGTGCCCGCGGAGGCGTGGCCGCGCACCGAGGGGGCCGTGGACT
TTGCGGCGCTATGACGGCCGCGGCGGGGGAGCGCGTGTGCGCGGCCGGGATCTACACGGCCTGGACGGAG
CCCGGCGCGCCGGGCGAGCTCCACGCGCTGGCGCACCTGCTGTGCCGCGACGGGGCCGGCGCGTACGCGG
CGCGCTTCGTCCACGTGACGGCGGCGCCGTGGACGGGCTCGGCGGCCGCGGTGGCCGCGGCGCTGCGCGA
CGCGAGCGGCACGGGGGCGCTGGCGGACCCGGCGCTGTGGCGCGGCGCGCACGGCGCGGTCATGGCGGCC
CTGCGCCGCGCCCTCCCGGCGCTGGCCTTCTACGAGCCGCTGCGCTTCGAGACGGACCCGGCCACGAACC
TGGTCACGGCCGCGACGCCGGCGGGCGGCGGCGGCGAAGAGGAGACGACCGGGGAGGGCGGCGAGGAGGA
CGAGGACGAGGCCGGCCCGCGCGCGGGCATCATCCACGTGGACGCCGACGTCGTCGTGGACCGCGACGCC
GCGCGCGCGCACGCCACCGAGGCGTGGCTGCCGCACGCCCAGCTGCGCGCCATGGCCGGCTGCGCCGCGC
TCTCCGTCGAGATCACGACGCGGCGGACCAGCTTCGCGCGCGCCTACGCGGAGGCCGGCGCGCCGCCGCT
GAAACGCCGGGGCGAGATGAGCGACCCCTTTGACGTGCGCGAGACGGTGCTGCGGCCCGCGGGCCGGCGC
GTGGCGGCGCGCGTGCTCGTCCCGCGCGGCTTCGACTGCCTGGTGGCCCACGGCGTGAGCAACGCGGCGC
TGGTGGCGCTCTACCGCCGGTGGCACGCGGCCGCGTACGGCGCGGGCGACGCGACCCCGCCCGTCTTCGC
CTTCCTGGGGCCCGAGCTCGCCCGCGAGGGCGCGGACGAGGACTACTACTGCGCGCTGGGCTTCCCCCTG
TTTGCGACGATCAAGGTGGCCGCGGCCGCGCCGGAGGCGGTCCGCGGCGCGCTCGCCGCCCACCGGCTCA
CGGACGGGCTGTGGCCCGCGCTCGGCTTCCGCGCCTTCCACGCCCTCGGGCCCGTGAGCTGCGGCCTGCG
CCTGCGCTGCGACGTCTGGCCGGCGGGGCGCGCGACCAGCGCCGTGGAGCACCCGTGCGCCCTCCGCGGG
GCCTGGCTGGCCAAGTTTGACTTCGCGGCCTTCTTCCCCAACCTGTTTCTGGCCCTGTGCCCGGGCCACG
AGCGGCTGCGGCGCGCCGTGCGGGCGCGCGCGGTCAAGCCGGCGCTCGTGGCCTTCTTCGGCGGGCTCAA
GCACACGTGCCCGGAGGCGTACCACAGCGTCATCGCGCTGGCCAACGGGGTCTCGCGCGCCGTCGAGGCG
GCCGCGGCCGAGGCCGGCCTGGTCACGTGCGCCTACGTCAAGGACGGCTTCTGGGGCGTGCTCGGCGACG
TGCCCGCGGCCGCGCCGGAGGCGGCCGCCGCCGCCGCGGCCGAGCGCGTGCGCGTCGCCTGCGAGGCCGC
CGCGGCGCGGCACCTGGCCGACGCGGGGGCCGCCGCGACGGACGTGACGCTGCGCTGCGAGGGCGTCTTC
GACGCGGCCCTGGCCTGGTCCTGCCACGCCTACTGGCTGCGGCGGCGCGAGGGCCCCGCGGACTTTGTCG
GCTTCCCGTCGCGCAGCGGCTTCGGCCGCGCCGCCAAGGCCGCGCTGGACGCCCTGCTGCGCGAGGCCGT
CGAGGGCCGCGCGGACGCCGAGGCCGCGCGCGCCGCCTGCGACGGCCTCGTCGAGCGGGCCTTCGCCTCG
CGCCACGACCCCGACTTCTGGGCCGCGCCGGGGGACGCCCTCGCCCCCGCCGCGTGCGCAGGGGCGACCG
CCGGGCGCGGCGGCGCGCGCTGCTCGCGGCGCTGCGTGCGCCTGCGCGGCGCCGGCCCGGTGCCCTACGA
GCAGCTGCGCCCCCCGCTGATCCTGCCCTGGATAGACTGTCTGGCCCACATGGAGCCCATCTTCGCCGCC
CTCGTCGGCATGTACAACCGCGCGCTCGAGGCGCTGAGCGCCGGCGACGCCCCGCCGCCATTTGTGTTTA
CATACACGCCGGACGCGTTTCTGTTTGGGTAAACAATAAAGTCTGTTTATTGTCAGAAC

Protein Sequence : Show Sequence

>gi|51557533|ref|YP_068367.1| UL8.5 protein [Suid herpesvirus 1]
MFAYVKPMAHGPDMASVYQSTGRVRRLLRDELFVYVDGSGARGEPIFTPVLLNHVVGSGWPARLSQVTNL
VCAQFQRRCRPAFAAARGMRLFSRFKFKHLFERCTLTSVNDSLNILHALLENNRLRVALEGCEPPLTARA
FCDFLRDARLDAFASQQVLRQLRPPDRPVAADIADSGEVATFVEKYLVADVPEDELQELLRALANPVTRE
QFVGLAVLGACARVPEALRSERVFGAVYGHYASGAVPVVADGRLELAALAPDFNVPARWALTRRCARVAE
AAGLFEGASPEVDSAAVAAAAADAELAPLLLEVLRCHVLDATTAARRPVRAALSALGAGGGAGPLSRGRH
AALVFKVMWEEAFGVRVGRSRQTFPGPTRVKNLRKAEIAALLRDAGLDPPAGATHRQLYALLMERRGDFA
GERYKLRLPAWSRLMYLTQGGFDAPLDAALSLVPAEAWPRTEGAVDFAAL

Molecule Role : Virmugen
Molecule Role Annotation : A gp50+gp63 deletion mutant in Pseudorabies virus was attenuated and provided protection from clinical signs of Aujeszky's disease after a challenge inoculation with the virulent wild-type PRV strain NIA-3 (Peeters et al., 1994).
Related Vaccine(s): NYVAC-PRV- gII/ gp50 , Pseudorabies virus gp50/gp63 mutant vaccine

10. gp63

Gene Name : gp63
Sequence Strain (Species/Organism) : Suid herpesvirus 1
NCBI Gene ID : 2952540
NCBI Protein GI : 51557548
Locus Tag : SuHV1_gp63
Genbank Accession : BK001744
Protein Accession : YP_068382
Taxonomy ID : 10345
Gene Starting Position : 102695
Gene Ending Position : 107803
Gene Strand (Orientation) : -
Protein Name : transcriptional regulator ICP4
Protein pI : 8.04
Protein Weight : 141692.15
Protein Length : 1446

DNA Sequence : Show Sequence

>gi|51557483:102695-107803 Suid herpesvirus 1, complete genome
CAGAAAATACAGCACAAACTTTATTTTCTCACTCTGAAAACAAACTCTTTTCTCACCCGATGGGAGAAGG
AGGAGAAGGGGACCGGGGGACCGCGGGAGGAGGAGAAGGGGACCGGGACGATCCTCCGCCGCCGAGCCCT
CCGCCGCGGCCGCCGCCGCCGCTTCCACCACCGCCGCCACCTCCGCCGCCGCCGCAGCCACCTCCGGCCG
GGGGATCCGCGCGGAGGAGAAGGAGAGGAGGAGGGCCACCGGGCCGGGGAGGCAGGCGCCGGGGAGGCAA
GCGCCGCCGGGCCGAGGGGACCGAGGCCGCCGCCGCGGACGCAGAGGAGGAGGAGGACGGGGACGGGGAC
GAGGACGAGGACGAGGACCGGGCCGAGGGCGAGGGGAGAGAAGACGGAGGAGAAGGGCCTCGAGGAGCCG
GTGGAGGGGCCGGAGAGTCAGAGTCAGAGTCCGGCCGGGCCGAGGGGGCGCCCCGCTCAGCGGAGCAGCA
GGTAGGGGTTGCCGGCGTCCTCGGCCTCCTCGTCGTCCGAGATGGCCTCCACCTTGATGGGCCCGAGCGG
GCCGCGGGGCCGGCCGTCGCCGCCGCGGACGCCGACGATCTCCACAGAGTCCCCGTCCTCGCCGGGGCCG
GCCCCGGCGCCCGAGGCCCCCGCGGGCCGGTGGGTCTCCACGGCGCCCCCGGCGGCGGCGCGGACGCTGG
TCTCGAAGGGGGCAAAGTCCCAGAGCACGGCCGGCGGGGCGCCCGCGGCGGCGACGGCGCCCGGGGTCAG
CACCAGCGGGGCGGCCTCGGCGTCGGGCTCCAGCAGCGCCGCGGCGCAGAAGGCGCGCAACTCGGCCGGC
AGGCCCTCGGGGCCGCGGAGCTCGGCGAGGCCCCGGCGGCCGCAGGAGACGAAGACGGGCCGCAGCGGGG
CGCCGAGCCCCCAGCGGTTGGCCGCGCGGTGCCCGAAGGCGGCGCCCGCGTCAAAGTCCGGGTCCCCGAG
CCCGAGCGCGGAGCGCTGGCGGGCCATGTCCTTGCAGCCGTCCACGGTGGGGAGCACGCGCTGGCGGTAG
GCGCGCGGCGGCAGCGGGACCGGGGTCCGGGGCCCGGCGCGGGTGCTCACCGTGTAGCGCACGTTGTCCT
GGCGGCAGAGGCGCAGCGGCTCGGCCCCGGGGTGCAGGCGGGCGAAGGAGGCCTCCACGCGGGCGAAGCA
GGCCGGGCCCACGATGGAGCTAGAGTCCAGCACGGCCGCGCGGAGCTCGCGGCACCCGGGCCAGCGCACG
GCGCACTGGGCGGCCGGGTCCAGGCGGACGCGGACGTAGACGTGGTAGTCCCCCACGGCCGGGCCGTCCG
CGGGCCAGTCCTCGATGGTGTCCAGCACGATGAGCCGGCGCCGCGCCGCGCCGAGCCGCGAGCAGAGGTA
CTCCACGGCGCCGGCGAAGCCGAGGTCCCGCGCCGAGAGCAGCAGCACCCCCTGGGCGTTGAGGCGGCCG
ATGTCGGGGCGCCCGGTCCAGTTCCCGGCCCAGGCGTGCGAGTCCGGCGTGCAGAGGCGGTGGGCGAAGG
CGGCGAGCAGCGCCGAGAGGCCGCCGCGGCGCGGGTCCCAGGCCGGGCGCGGGGCGCCCTCGGCGGGCTC
GGCGCAGAGCTCCTCGTGGGGCAGCGGGTCGTAGAGCACCACCACGCGCACGTCCTCGGGGTCGGCTATC
TGCCGCATCCAGGCGGCGCGGCGGCGGAGCGGGGCGCCGCGGCGCGCGGCGATGTGCGCCAGGGCGGCCG
GGTCGAAGGTGAGCGCCGGGCGCCAGAGTTCGGGGAAGACCTCCTGGTCCACGAGGGCGCGGGCCACCTC
GGGCGGGCAGTAGGCGGCGAGGGCCGCCTCGGAGGGCCGCGGCGTGTGGGTCTCGCCGGCCGGGACGCGG
CGGAAGCCGCCGTCGGGCGCGGGGTGCTCGGGCATGGGCCCGAGCGGGCGCCGGAGCCGGTCGTCCTCGG
AGGAGGAGGAGGAGGAGGACAGCAGCGCGGGAGCGGGGTCCGGAGCGGGCCCGAGTCCGAGGGAGCGGCG
CTTGCGCCGGGGCCCCCGGTCCTCTTCGTCGTCGCGGTGGCCGTGGCCGTCCCCGCGGAGGGCCGCGCCG
GAGAGCCCCTCGTCCTCCTCGCCGTCCCCGGGGCGGCGGGCCCCGGGCGCGCGGCGCTTCTTCTTGCGCC
GCTCGGGCGCTGGGTCCGGGCCGGCGGCGGGGGAGCTGGCGTAGCCGGAGGAGCCGGAGAGGCCGGACTT
GGTGCTGGAGCTGGACTTGGTGCTGGAGCTGGACTTGGTGCTGGCGGGGCTGGAGGGCCCGGAGCCGGGG
AGGCCGGAGGGGGCGCCCGCCGCCGCCGGCGCCGGCGCTGGGACGACGAGGCCGGGCTGCTCGGGCCAGA
GCGGGGGCAGGCCGGGCGCGGGCTCCGCGGGCCCGGGCCGCGCGGCGGCCTCGGCGAGCCGGGCCGCGGC
CACGTTGGCCGGGGCGAAGAGGGCCGCGGCGTAGGTCCAGGCGGCCTCGCGGGCGCGGGCCCCGTCCACG
CTGTAGCGCACCAGCGGCGCCACGGTGCGGGCGACGAGGGCGACAGAGTCCGCGGCCTGCCGCCGCTCGG
CCGGGCCGGCCCCGGGGATCGCGTCGCGGAGCGCGAGCAGCGCGGCCGTCACCTCCTCGAGGCAGGCGGG
CCCGAGGGCGGCCGGGGCGCGGGCGGGCGCGGGCAGCCGGAGCGGGCAGGGCAGCAGGCGCTCGAGGACG
CCGCGGCAGGCCAGGACGCAGGCGTCCGCCAGCTCGCGGGGCACGCGGCCGGGCTGCGCGGCGGCGAAGG
CGGCGCGGACGCGGGCGCAGAGGGCCTCGACGGTCGCCTCCCCGGCGCGGGGGTCCGCGGCGCGGCCCGG
GTAGGCCATGTCGGCGTAGGCCCGGCGGAGGCTCTGCAGGATGAAGGTCTTCTGGGTGCGATCGTAGCGG
CGGCTCATGGCCACGGCGCTCACCGCGTGCGGCAGGGCCCAGAGCGGGTCCTGGGCGGCCATGGCGTCCC
CGATGTGCGGCAGCGGCGGGGTCACGCTGCCGGTGATGAAGGAGCCGTGGCCGTGGGGCGCGTGGACCCG
GCGCTGGCAGAACTGGTTGAAGCGCTGGTCGGGGGCCTGCATCCGCGGGTTCTGCAGCCAGGACATGGCC
TCGCCGGCGGCCCCGCTGTAGATGAGGCGCACGAGGGCCTCGTGCTGCTTCCTCGAGTCCCCCATCTCCG
GGATGAAGACGGGCACGGGCCCGGCCGCGGCGCGGTAGCGGGCCGCGGCCTGGCGGACGTCGTCCTCGTC
CCAGAGCCCCTCGCGGGAGTCCCCGGCGCCGCCGTAGCGGACGCGGCCGTCGGCCGGAGGGTCGGAGCCG
GGCCAGGGCTCCCCGAGCGGGGTGAGCAGCGGCCCGTCGGTCGGCGGGGGCCCGTCGGCCATGAGCGAGA
GGTGGTTGTTGGTGGAGCGGCGCTTCCTGCGCGGGGGCCGGGCGGGCTCCGGGGCCGGGGCCGGGGAGGC
CGCGGCGGAGGAGGAGGCGGAGGAGGCCGAGGGCCGCGGGGCCGCGGCGGGCGCCGGCGGAGACGGTGGC
GGCCCGGCGCGGGCGAGTGGGGCGCCGGGCCGGACTCCTTCGTCTTCTTCTCCCTCGGAGGAGGACGAGG
ACGAGGAGGACGAGGAGGACGAGGAGGACGAGGACGAGGAGGAGGCCGAGCGCCGCGCGGCGGCGGCGGG
GGCCCGGGGGGCGGAGGGCGAGCGGGCCGGGGAGAGGTCCGAGTCGCTGCCGCTGCTGCTGGAGCTGCTG
AAGCCGCGGCCGCGGCGGAGGGCGCCCTCTCCGGCGCGGCGCCGGCGGGGCTGTCTCTGCGGGGGCGCCC
CGCCGTCCCCGGCGAGGCCGAGTCCGTCCTCGTCCTTCTCGGGGCCGCGGGCGACGGTCTCGACGGCGAC
GGTGGTGGTGGAGTTGGAGTTGGAGTTGGGGTTGGAGGAGACGGGGCTCCGGGCGCCAAGCGGCCGAGGA
TCGAGCCGCCTCGCGGCGGCGGGCTCGTCGAGCAGGGGCTCGCGGTGCTGGTGATGGTGACGACCGCGGT
CCCCTCCGGCGGAGGGGGCGCCGCCGCCGCCGGGCGCCGAGACCGGCCCGGCGGCGGGGGAGGCTGGGGA
AGCGGGCCCCCGCCGTGCCGGCGCTGCGGCCACCGCTGCTGGCTGTGCTGGTGGCGCCGGGGTCCGAGGC
CGCGCCGCCGGCCCGGGCTCACCGACCGGGTCCCCCCTCGCGGGGGACCATCTCCGCGGGGCTGCCGAGG
GGCCGGGAGAGCCGGAGGAGGAGGCCGGGGAGGCTGCGGAGGGGGACGAGCGCCCGGGGCCGCCGGGGGC
CCCGGCCTCTGCCGCTGCGAGTGCTGCCGGGGTCGGCGGCCGGGGCCCGGAGCCGGCCCGGGACCGGGGC
CCGAGGACGAGGTGACCGTGCTCGGAGCCCTGATGGAGAGCCCGACCGGGGGACCCGGCGGCCGGGGACC
CGGGCTCGTCCTCCTCCTCGTCTTCGTCGTCTAGCACCACGATCTCGCCCGAGCCCCGGCGGGGCTGCTG
CTGCTGCTGGGCCGAAGGAGGACGGGGCGGCCTCGTGGCTCCGGCCGCGGCCGCGAGGACGGCGGCCTCG
GCCTCGGCGGCGTCGTCGGAGAAGAGGCCGCCCGGGCCGAAGAGGAGATCCTCGCCGGAGGAGCCGCGGC
GCCGGGAGCCCTGGCTGCCGCCGTCGGGGCCGGACGCGATGCCCTCTTCCTCGGCCGCGGCGGCGGCCGC
CAGGAGCTGGCTGAAGTTGCCCTCGGTCTCGATGAAGTCAAAGAGATCGTCGGCCATGGTCTCGATCGGG
GTCTTTCTGCCTGAGCGAGGCCGGGCGCCGAGCGCGGAGAGCGGGCGGCGGAGAAGAAGGAGGAAGGCGG
CCGGAGGAGGAGAAGAAGAAGACTCTTCTCTGGCGGGCCGAGAGCCTCGGTGGGTCGGGCGTCCGTCGAG
GGCTGATAGCCGCCGGAGAGCCGGAGTCTTCAGAGTCCGCGCCGGAGCGGAGACGGTCGGATCCCCTCGG
GTTGGCAGAGAACGATGCTGTCCGTACCTGCACCGCAGTGAAGTGCTACGATGGAGACCGCGCTTATAA

Protein Sequence : Show Sequence

>gi|51557548|ref|YP_068382.1| transcriptional regulator ICP4 [Suid herpesvirus 1]
MADDLFDFIETEGNFSQLLAAAAAAEEEGIASGPDGGSQGSRRRGSSGEDLLFGPGGLFSDDAAEAEAAV
LAAAAGATRPPRPPSAQQQQQPRRGSGEIVVLDDEDEEEDEPGSPAAGSPGRALHQGSEHGHLVLGPRSR
AGSGPRPPTPAALAAAEAGAPGGPGRSSPSAASPASSSGSPGPSAAPRRWSPARGDPVGEPGPAARPRTP
APPAQPAAVAAAPARRGPASPASPAAGPVSAPGGGGAPSAGGDRGRHHHQHREPLLDEPAAARRLDPRPL
GARSPVSSNPNSNSNSTTTVAVETVARGPEKDEDGLGLAGDGGAPPQRQPRRRRAGEGALRRGRGFSSSS
SSGSDSDLSPARSPSAPRAPAAAARRSASSSSSSSSSSSSSSSSSSSEGEEDEGVRPGAPLARAGPPPSP
PAPAAAPRPSASSASSSAAASPAPAPEPARPPRRKRRSTNNHLSLMADGPPPTDGPLLTPLGEPWPGSDP
PADGRVRYGGAGDSREGLWDEDDVRQAAARYRAAAGPVPVFIPEMGDSRKQHEALVRLIYSGAAGEAMSW
LQNPRMQAPDQRFNQFCQRRVHAPHGHGSFITGSVTPPLPHIGDAMAAQDPLWALPHAVSAVAMSRRYDR
TQKTFILQSLRRAYADMAYPGRAADPRAGEATVEALCARVRAAFAAAQPGRVPRELADACVLACRGVLER
LLPCPLRLPAPARAPAALGPACLEEVTAALLALRDAIPGAGPAERRQAADSVALVARTVAPLVRYSVDGA
RAREAAWTYAAALFAPANVAAARLAEAAARPGPAEPAPGLPPLWPEQPGLVVPAPAPAAAGAPSGLPGSG
PSSPASTKSSSSTKSSSSTKSGLSGSSGYASSPAAGPDPAPERRKKKRRAPGARRPGDGEEDEGLSGAAL
RGDGHGHRDDEEDRGPRRKRRSLGLGPAPDPAPALLSSSSSSSEDDRLRRPLGPMPEHPAPDGGFRRVPA
GETHTPRPSEAALAAYCPPEVARALVDQEVFPELWRPALTFDPAALAHIAARRGAPLRRRAAWMRQIADP
EDVRVVVLYDPLPHEELCAEPAEGAPRPAWDPRRGGLSALLAAFAHRLCTPDSHAWAGNWTGRPDIGRLN
AQGVLLLSARDLGFAGAVEYLCSRLGAARRRLIVLDTIEDWPADGPAVGDYHVYVRVRLDPAAQCAVRWP
GCRELRAAVLDSSSIVGPACFARVEASFARLHPGAEPLRLCRQDNVRYTVSTRAGPRTPVPLPPRAYRQR
VLPTVDGCKDMARQRSALGLGDPDFDAGAAFGHRAANRWGLGAPLRPVFVSCGRRGLAELRGPEGLPAEL
RAFCAAALLEPDAEAAPLVLTPGAVAAAGAPPAVLWDFAPFETSVRAAAGGAVETHRPAGASGAGAGPGE
DGDSVEIVGVRGGDGRPRGPLGPIKVEAISDDEEAEDAGNPYLLLR

Molecule Role : Virmugen
Molecule Role Annotation : A gp50+gp63 deletion mutant in Pseudorabies virus was attenuated and provided protection from clinical signs of Aujeszky's disease after a challenge inoculation with the virulent wild-type PRV strain NIA-3 (Peeters et al., 1994).
Related Vaccine(s): Pseudorabies virus gp50/gp63 mutant vaccine

11. IE180

Gene Name : IE180
Sequence Strain (Species/Organism) : Suid alphaherpesvirus 1
VO ID : VO_0011242
NCBI Gene ID : 2952540
NCBI Protein GI : 51557548
Locus Tag : SuHV1_gp63
Genbank Accession : BK001744
Protein Accession : YP_068382
Taxonomy ID : 10345
Gene Starting Position : 102695
Gene Ending Position : 107803
Gene Strand (Orientation) : -
Protein Name : transcriptional regulator ICP4
Protein pI : 8.04
Protein Weight : 141692.15
Protein Length : 1446
Protein Note : transcriptional regulator ICP4; the Herpesviridae are non-segmented dsDNA viruses with genomes ranging from 120-230kbp; although herpes viruses vary greatly in sequence identity and homology, they all share four common elements: an envelope, a tegument which is composed of viral enzymes, a capsid of 162 capsomers, and a core composed of genomic DNA; transcriptional regulator ICP4 ( infected-cell polypeptide 4) is a sequence-specific transcriptional activator that is required for efficient transcription of early and late viral genes; ICP4 is essential for productive infection

DNA Sequence : Show Sequence

>NC_006151.1:102695-107803 Suid herpesvirus 1, complete genome
CAGAAAATACAGCACAAACTTTATTTTCTCACTCTGAAAACAAACTCTTTTCTCACCCGATGGGAGAAGG
AGGAGAAGGGGACCGGGGGACCGCGGGAGGAGGAGAAGGGGACCGGGACGATCCTCCGCCGCCGAGCCCT
CCGCCGCGGCCGCCGCCGCCGCTTCCACCACCGCCGCCACCTCCGCCGCCGCCGCAGCCACCTCCGGCCG
GGGGATCCGCGCGGAGGAGAAGGAGAGGAGGAGGGCCACCGGGCCGGGGAGGCAGGCGCCGGGGAGGCAA
GCGCCGCCGGGCCGAGGGGACCGAGGCCGCCGCCGCGGACGCAGAGGAGGAGGAGGACGGGGACGGGGAC
GAGGACGAGGACGAGGACCGGGCCGAGGGCGAGGGGAGAGAAGACGGAGGAGAAGGGCCTCGAGGAGCCG
GTGGAGGGGCCGGAGAGTCAGAGTCAGAGTCCGGCCGGGCCGAGGGGGCGCCCCGCTCAGCGGAGCAGCA
GGTAGGGGTTGCCGGCGTCCTCGGCCTCCTCGTCGTCCGAGATGGCCTCCACCTTGATGGGCCCGAGCGG
GCCGCGGGGCCGGCCGTCGCCGCCGCGGACGCCGACGATCTCCACAGAGTCCCCGTCCTCGCCGGGGCCG
GCCCCGGCGCCCGAGGCCCCCGCGGGCCGGTGGGTCTCCACGGCGCCCCCGGCGGCGGCGCGGACGCTGG
TCTCGAAGGGGGCAAAGTCCCAGAGCACGGCCGGCGGGGCGCCCGCGGCGGCGACGGCGCCCGGGGTCAG
CACCAGCGGGGCGGCCTCGGCGTCGGGCTCCAGCAGCGCCGCGGCGCAGAAGGCGCGCAACTCGGCCGGC
AGGCCCTCGGGGCCGCGGAGCTCGGCGAGGCCCCGGCGGCCGCAGGAGACGAAGACGGGCCGCAGCGGGG
CGCCGAGCCCCCAGCGGTTGGCCGCGCGGTGCCCGAAGGCGGCGCCCGCGTCAAAGTCCGGGTCCCCGAG
CCCGAGCGCGGAGCGCTGGCGGGCCATGTCCTTGCAGCCGTCCACGGTGGGGAGCACGCGCTGGCGGTAG
GCGCGCGGCGGCAGCGGGACCGGGGTCCGGGGCCCGGCGCGGGTGCTCACCGTGTAGCGCACGTTGTCCT
GGCGGCAGAGGCGCAGCGGCTCGGCCCCGGGGTGCAGGCGGGCGAAGGAGGCCTCCACGCGGGCGAAGCA
GGCCGGGCCCACGATGGAGCTAGAGTCCAGCACGGCCGCGCGGAGCTCGCGGCACCCGGGCCAGCGCACG
GCGCACTGGGCGGCCGGGTCCAGGCGGACGCGGACGTAGACGTGGTAGTCCCCCACGGCCGGGCCGTCCG
CGGGCCAGTCCTCGATGGTGTCCAGCACGATGAGCCGGCGCCGCGCCGCGCCGAGCCGCGAGCAGAGGTA
CTCCACGGCGCCGGCGAAGCCGAGGTCCCGCGCCGAGAGCAGCAGCACCCCCTGGGCGTTGAGGCGGCCG
ATGTCGGGGCGCCCGGTCCAGTTCCCGGCCCAGGCGTGCGAGTCCGGCGTGCAGAGGCGGTGGGCGAAGG
CGGCGAGCAGCGCCGAGAGGCCGCCGCGGCGCGGGTCCCAGGCCGGGCGCGGGGCGCCCTCGGCGGGCTC
GGCGCAGAGCTCCTCGTGGGGCAGCGGGTCGTAGAGCACCACCACGCGCACGTCCTCGGGGTCGGCTATC
TGCCGCATCCAGGCGGCGCGGCGGCGGAGCGGGGCGCCGCGGCGCGCGGCGATGTGCGCCAGGGCGGCCG
GGTCGAAGGTGAGCGCCGGGCGCCAGAGTTCGGGGAAGACCTCCTGGTCCACGAGGGCGCGGGCCACCTC
GGGCGGGCAGTAGGCGGCGAGGGCCGCCTCGGAGGGCCGCGGCGTGTGGGTCTCGCCGGCCGGGACGCGG
CGGAAGCCGCCGTCGGGCGCGGGGTGCTCGGGCATGGGCCCGAGCGGGCGCCGGAGCCGGTCGTCCTCGG
AGGAGGAGGAGGAGGAGGACAGCAGCGCGGGAGCGGGGTCCGGAGCGGGCCCGAGTCCGAGGGAGCGGCG
CTTGCGCCGGGGCCCCCGGTCCTCTTCGTCGTCGCGGTGGCCGTGGCCGTCCCCGCGGAGGGCCGCGCCG
GAGAGCCCCTCGTCCTCCTCGCCGTCCCCGGGGCGGCGGGCCCCGGGCGCGCGGCGCTTCTTCTTGCGCC
GCTCGGGCGCTGGGTCCGGGCCGGCGGCGGGGGAGCTGGCGTAGCCGGAGGAGCCGGAGAGGCCGGACTT
GGTGCTGGAGCTGGACTTGGTGCTGGAGCTGGACTTGGTGCTGGCGGGGCTGGAGGGCCCGGAGCCGGGG
AGGCCGGAGGGGGCGCCCGCCGCCGCCGGCGCCGGCGCTGGGACGACGAGGCCGGGCTGCTCGGGCCAGA
GCGGGGGCAGGCCGGGCGCGGGCTCCGCGGGCCCGGGCCGCGCGGCGGCCTCGGCGAGCCGGGCCGCGGC
CACGTTGGCCGGGGCGAAGAGGGCCGCGGCGTAGGTCCAGGCGGCCTCGCGGGCGCGGGCCCCGTCCACG
CTGTAGCGCACCAGCGGCGCCACGGTGCGGGCGACGAGGGCGACAGAGTCCGCGGCCTGCCGCCGCTCGG
CCGGGCCGGCCCCGGGGATCGCGTCGCGGAGCGCGAGCAGCGCGGCCGTCACCTCCTCGAGGCAGGCGGG
CCCGAGGGCGGCCGGGGCGCGGGCGGGCGCGGGCAGCCGGAGCGGGCAGGGCAGCAGGCGCTCGAGGACG
CCGCGGCAGGCCAGGACGCAGGCGTCCGCCAGCTCGCGGGGCACGCGGCCGGGCTGCGCGGCGGCGAAGG
CGGCGCGGACGCGGGCGCAGAGGGCCTCGACGGTCGCCTCCCCGGCGCGGGGGTCCGCGGCGCGGCCCGG
GTAGGCCATGTCGGCGTAGGCCCGGCGGAGGCTCTGCAGGATGAAGGTCTTCTGGGTGCGATCGTAGCGG
CGGCTCATGGCCACGGCGCTCACCGCGTGCGGCAGGGCCCAGAGCGGGTCCTGGGCGGCCATGGCGTCCC
CGATGTGCGGCAGCGGCGGGGTCACGCTGCCGGTGATGAAGGAGCCGTGGCCGTGGGGCGCGTGGACCCG
GCGCTGGCAGAACTGGTTGAAGCGCTGGTCGGGGGCCTGCATCCGCGGGTTCTGCAGCCAGGACATGGCC
TCGCCGGCGGCCCCGCTGTAGATGAGGCGCACGAGGGCCTCGTGCTGCTTCCTCGAGTCCCCCATCTCCG
GGATGAAGACGGGCACGGGCCCGGCCGCGGCGCGGTAGCGGGCCGCGGCCTGGCGGACGTCGTCCTCGTC
CCAGAGCCCCTCGCGGGAGTCCCCGGCGCCGCCGTAGCGGACGCGGCCGTCGGCCGGAGGGTCGGAGCCG
GGCCAGGGCTCCCCGAGCGGGGTGAGCAGCGGCCCGTCGGTCGGCGGGGGCCCGTCGGCCATGAGCGAGA
GGTGGTTGTTGGTGGAGCGGCGCTTCCTGCGCGGGGGCCGGGCGGGCTCCGGGGCCGGGGCCGGGGAGGC
CGCGGCGGAGGAGGAGGCGGAGGAGGCCGAGGGCCGCGGGGCCGCGGCGGGCGCCGGCGGAGACGGTGGC
GGCCCGGCGCGGGCGAGTGGGGCGCCGGGCCGGACTCCTTCGTCTTCTTCTCCCTCGGAGGAGGACGAGG
ACGAGGAGGACGAGGAGGACGAGGAGGACGAGGACGAGGAGGAGGCCGAGCGCCGCGCGGCGGCGGCGGG
GGCCCGGGGGGCGGAGGGCGAGCGGGCCGGGGAGAGGTCCGAGTCGCTGCCGCTGCTGCTGGAGCTGCTG
AAGCCGCGGCCGCGGCGGAGGGCGCCCTCTCCGGCGCGGCGCCGGCGGGGCTGTCTCTGCGGGGGCGCCC
CGCCGTCCCCGGCGAGGCCGAGTCCGTCCTCGTCCTTCTCGGGGCCGCGGGCGACGGTCTCGACGGCGAC
GGTGGTGGTGGAGTTGGAGTTGGAGTTGGGGTTGGAGGAGACGGGGCTCCGGGCGCCAAGCGGCCGAGGA
TCGAGCCGCCTCGCGGCGGCGGGCTCGTCGAGCAGGGGCTCGCGGTGCTGGTGATGGTGACGACCGCGGT
CCCCTCCGGCGGAGGGGGCGCCGCCGCCGCCGGGCGCCGAGACCGGCCCGGCGGCGGGGGAGGCTGGGGA
AGCGGGCCCCCGCCGTGCCGGCGCTGCGGCCACCGCTGCTGGCTGTGCTGGTGGCGCCGGGGTCCGAGGC
CGCGCCGCCGGCCCGGGCTCACCGACCGGGTCCCCCCTCGCGGGGGACCATCTCCGCGGGGCTGCCGAGG
GGCCGGGAGAGCCGGAGGAGGAGGCCGGGGAGGCTGCGGAGGGGGACGAGCGCCCGGGGCCGCCGGGGGC
CCCGGCCTCTGCCGCTGCGAGTGCTGCCGGGGTCGGCGGCCGGGGCCCGGAGCCGGCCCGGGACCGGGGC
CCGAGGACGAGGTGACCGTGCTCGGAGCCCTGATGGAGAGCCCGACCGGGGGACCCGGCGGCCGGGGACC
CGGGCTCGTCCTCCTCCTCGTCTTCGTCGTCTAGCACCACGATCTCGCCCGAGCCCCGGCGGGGCTGCTG
CTGCTGCTGGGCCGAAGGAGGACGGGGCGGCCTCGTGGCTCCGGCCGCGGCCGCGAGGACGGCGGCCTCG
GCCTCGGCGGCGTCGTCGGAGAAGAGGCCGCCCGGGCCGAAGAGGAGATCCTCGCCGGAGGAGCCGCGGC
GCCGGGAGCCCTGGCTGCCGCCGTCGGGGCCGGACGCGATGCCCTCTTCCTCGGCCGCGGCGGCGGCCGC
CAGGAGCTGGCTGAAGTTGCCCTCGGTCTCGATGAAGTCAAAGAGATCGTCGGCCATGGTCTCGATCGGG
GTCTTTCTGCCTGAGCGAGGCCGGGCGCCGAGCGCGGAGAGCGGGCGGCGGAGAAGAAGGAGGAAGGCGG
CCGGAGGAGGAGAAGAAGAAGACTCTTCTCTGGCGGGCCGAGAGCCTCGGTGGGTCGGGCGTCCGTCGAG
GGCTGATAGCCGCCGGAGAGCCGGAGTCTTCAGAGTCCGCGCCGGAGCGGAGACGGTCGGATCCCCTCGG
GTTGGCAGAGAACGATGCTGTCCGTACCTGCACCGCAGTGAAGTGCTACGATGGAGACCGCGCTTATAA

Protein Sequence : Show Sequence

>YP_068382.1 transcriptional regulator ICP4 [Suid alphaherpesvirus 1]
MADDLFDFIETEGNFSQLLAAAAAAEEEGIASGPDGGSQGSRRRGSSGEDLLFGPGGLFSDDAAEAEAAV
LAAAAGATRPPRPPSAQQQQQPRRGSGEIVVLDDEDEEEDEPGSPAAGSPGRALHQGSEHGHLVLGPRSR
AGSGPRPPTPAALAAAEAGAPGGPGRSSPSAASPASSSGSPGPSAAPRRWSPARGDPVGEPGPAARPRTP
APPAQPAAVAAAPARRGPASPASPAAGPVSAPGGGGAPSAGGDRGRHHHQHREPLLDEPAAARRLDPRPL
GARSPVSSNPNSNSNSTTTVAVETVARGPEKDEDGLGLAGDGGAPPQRQPRRRRAGEGALRRGRGFSSSS
SSGSDSDLSPARSPSAPRAPAAAARRSASSSSSSSSSSSSSSSSSSSEGEEDEGVRPGAPLARAGPPPSP
PAPAAAPRPSASSASSSAAASPAPAPEPARPPRRKRRSTNNHLSLMADGPPPTDGPLLTPLGEPWPGSDP
PADGRVRYGGAGDSREGLWDEDDVRQAAARYRAAAGPVPVFIPEMGDSRKQHEALVRLIYSGAAGEAMSW
LQNPRMQAPDQRFNQFCQRRVHAPHGHGSFITGSVTPPLPHIGDAMAAQDPLWALPHAVSAVAMSRRYDR
TQKTFILQSLRRAYADMAYPGRAADPRAGEATVEALCARVRAAFAAAQPGRVPRELADACVLACRGVLER
LLPCPLRLPAPARAPAALGPACLEEVTAALLALRDAIPGAGPAERRQAADSVALVARTVAPLVRYSVDGA
RAREAAWTYAAALFAPANVAAARLAEAAARPGPAEPAPGLPPLWPEQPGLVVPAPAPAAAGAPSGLPGSG
PSSPASTKSSSSTKSSSSTKSGLSGSSGYASSPAAGPDPAPERRKKKRRAPGARRPGDGEEDEGLSGAAL
RGDGHGHRDDEEDRGPRRKRRSLGLGPAPDPAPALLSSSSSSSEDDRLRRPLGPMPEHPAPDGGFRRVPA
GETHTPRPSEAALAAYCPPEVARALVDQEVFPELWRPALTFDPAALAHIAARRGAPLRRRAAWMRQIADP
EDVRVVVLYDPLPHEELCAEPAEGAPRPAWDPRRGGLSALLAAFAHRLCTPDSHAWAGNWTGRPDIGRLN
AQGVLLLSARDLGFAGAVEYLCSRLGAARRRLIVLDTIEDWPADGPAVGDYHVYVRVRLDPAAQCAVRWP
GCRELRAAVLDSSSIVGPACFARVEASFARLHPGAEPLRLCRQDNVRYTVSTRAGPRTPVPLPPRAYRQR
VLPTVDGCKDMARQRSALGLGDPDFDAGAAFGHRAANRWGLGAPLRPVFVSCGRRGLAELRGPEGLPAEL
RAFCAAALLEPDAEAAPLVLTPGAVAAAGAPPAVLWDFAPFETSVRAAAGGAVETHRPAGASGAGAGPGE
DGDSVEIVGVRGGDGRPRGPLGPIKVEAISDDEEAEDAGNPYLLLR

Molecule Role : Protective antigen
Molecule Role Annotation : To examine the response by IE180 more closely, a vaccine trial in mice with a vector DNA construct that contains the gene encoding for IE180, designated pcDNAIE180 was conducted. Seven months after immunization with pcDNAIE180, an overall 25% of BALB/c, C3H/HeJ, and C57BL/6 mice receiving a lethal PrV challenge were protected (Chang et al., 1998).
Related Vaccine(s): Psuedorabies DNA vaccine encoding IE180 protein

12. UL23

Gene Name : UL23
Sequence Strain (Species/Organism) : Suid herpesvirus 1
NCBI Gene ID : 2952559
NCBI Protein GI : 51557518
Locus Tag : SuHV1_gp35
Genbank Accession : BK001744
Protein Accession : YP_068352
Taxonomy ID : 10345
Gene Starting Position : 59511
Gene Ending Position : 60589
Gene Strand (Orientation) : +
Protein Name : thymidine kinase
Protein pI : 7.14
Protein Weight : 32400.33
Protein Length : 320
Protein Note : 2.7.1.21

DNA Sequence : Show Sequence

>gi|51557483:59511-60589 Suid herpesvirus 1, complete genome
GATGCGCATCCTCCGGATCTACCTCGACGGCGCCTACGGCACCGGCAAGAGCACCACTGCCCGGGTGATG
GCGCTCGGCGGGGCGCTGTACGTGCCCGAGCCGATGGCGTACTGGCGCACTCTGTTCGACACGGACACGG
TGGCCGGTATTTACGATGCGCAGACCCGGAAGCAGAACGGCAGCCTGAGCGAGGAGGACGCGGCCCTCGT
CACGGCGCAGCACCAGGCCGCCTTCGCGACGCCGTACCTGCTGCTGCACACGCGCCTGGTCCCGCTCTTC
GGGCCCGCGGTCGAGGGCCCGCCCGAGATGACGGTCGTCTTTGACCGCCACCCGGTGGCCGCGACGGTGT
GCTTCCCGCTGGCGCGCTTCATCGTCGGGGACATCAGCGCGGCGGCCTTCGTGGGCCTGGCGGCCACGCT
GCCCGGGGAGCCCCCCGGCGGCAACCTGGTGGTGGCCTCGCTGGACCCGGACGAGCACCTGCGGCGCCTG
CGCGCCCGCGCGCGCGCCGGGGAGCACGTGGACGCGCGCCTGCTCACGGCCCTGCGCAACGTCTACGCCA
TGCTGGTCAACACGTCGCGCTACCTGAGCTCGGGGCGCCGCTGGCGCGACGACTGGGGGCGCGCGCCGCG
CTTCGACCAGACCACGCGGGACTGCCTCGCGCTCAACGAGCTCTGCCGCCCGCGCGACGACCCCGAGCTC
CAGGACACCCTCTTTGGCGCGTACAAGGCGCCCGAGCTCTGCGACCGGCGCGGGCGCCCGCTCGAGGTGC
ACGCGTGGGCGATGGACGCGCTCGTGGCCAAGCTGCTGCCGCTGCGCGTCTCCACCGTCGACCTGGGGCC
CTCGCCGCGCGTCTGCGCCGCGGCCGTGGCGGCGCAGACGCGCGGCATGGAGGTGACGGAGTCCGCGTAC
GGCGACCACATCCGGCAGTGCGTGTGCGCCTTCACGTCGGAGATGGGGGTGTGACCCTCGCCCCTCCCAC
CCGCGCCGCGGCCAGATGGAGACCGCGACGGAGGCAACGACGACGGCGTGGGAGGGGGCTCGGGGCGCGT
ATAAAGCTATGTGTATGTCATCCCAATAA

Protein Sequence : Show Sequence

>gi|51557518|ref|YP_068352.1| thymidine kinase [Suid herpesvirus 1]
MRILRIYLDGAYGTGKSTTARVMALGGALYVPEPMAYWRTLFDTDTVAGIYDAQTRKQNGSLSEEDAALV
TAQHQAAFATPYLLLHTRLVPLFGPAVEGPPEMTVVFDRHPVAATVCFPLARFIVGDISAAAFVGLAATL
PGEPPGGNLVVASLDPDEHLRRLRARARAGEHVDARLLTALRNVYAMLVNTSRYLSSGRRWRDDWGRAPR
FDQTTRDCLALNELCRPRDDPELQDTLFGAYKAPELCDRRGRPLEVHAWAMDALVAKLLPLRVSTVDLGP
SPRVCAAAVAAQTRGMEVTESAYGDHIRQCVCAFTSEMGV

Molecule Role : Virmugen
Molecule Role Annotation : A UL23 mutant, in combination with mutations in glycoproteins G and E, is attenuated in raccoons, with a 75% survival rate. This mutant also provides some protection from wild type PRV (Weigel et al., 2003).
Related Vaccine(s): Pseudorabies virus glycoprotein E/G/UL23 mutant vaccine

13. UL44

Gene Name : UL44
Sequence Strain (Species/Organism) : Suid alphaherpesvirus 1
VO ID : VO_0011239
NCBI Gene ID : 2952505
NCBI Protein GI : 51557513
Locus Tag : SuHV1_gp30
Genbank Accession : BK001744
Protein Accession : YP_068347
Taxonomy ID : 10345
Gene Starting Position : 53985
Gene Ending Position : 55504
Gene Strand (Orientation) : +
Protein Name : envelope glycoprotein C
Protein pI : 6.98
Protein Weight : 45878.36
Protein Length : 479
Protein Note : envelope glycoprotein C; the Herpesviridae are non-segmented dsDNA viruses with genomes ranging from 120-230kbp; although herpes viruses vary greatly in sequence identity and homology, they all share four common elements: an envelope, a tegument which is composed of viral enzymes, a capsid of 162 capsomers, and a core composed of genomic DNA;virion envelope glycoproteins bind to cellular receptors; the nonessential glycoprotein gC interacts with cell surface proteoglycans, whereas the essential glycoprotein gD is involved in stable secondary attachment

DNA Sequence : Show Sequence

>NC_006151.1:53985-55504 Suid herpesvirus 1, complete genome
TTTTAAAACCGCGATGGGGGGACGGGGGGCCATTCGCACGCGCCATGGCCTCGCTCGCGCGTGCGATGCT
CGCTCTGCTGGCGCTCTACGCGGCGGCCATCGCCGCGGCGCCGTCGACCACGACGGCGCTCGACACGACG
CCCAACGGGGGCGGCGGCGGCAACAGCAGCGAGGGAGAACTCTCGCCCTCTCCGCCCCCGACCCCCGCGC
CCGCCTCGCCCGAGGCGGGCGCGGTCTCGACGCCCCCGGTCCCGCCGCCCTCGGTCTCGCGCAGGAAGCC
CCCGCGGAACAACAACCGGACGCGCGTCCACGGCGACAAGGCCACCGCGCACGGGCGCAAGCGCATCGTG
TGCCGGGAGCGGCTGTTCTCGGCGCGGGTGGGGGACGCGGTCAGCTTCGGGTGCGCCGTCTTCCCGCGCG
CCGGGGAGACCTTCGAGGTCCGCTTCTACCGCCGCGGGCGCTTCCGCTCGCCCGACGCCGACCCCGAGTA
CTTTGACGAGCCCCCGCGCCCGGAGCTCCCGCGGGAGCGGCTCCTCTTCAGCTCCGCCAACGCCTCCCTC
GCCCACGCGGACGCGCTCGCGCCCGTCGTCGTCGAGGGCGAGCGCGCGACCGTCGCCAACGTCTCGGGCG
AGGTGTCCGTGCGCGTGGCCGCGGCGGACGCCGAGACCGAGGGCGTCTACACGTGGCGCGTGCTGTCCGC
CAACGGCACCGAGGTCCGGAGCGCCAACGTCTCGCTCCTCCTGTACAGCCAGCCCGAGTTCGGCCTGAGC
GCGCCGCCCGTCCTCTTCGGTGAGCCCTTCCGGGCGGTGTGCGTCGTCCGCGACTACTACCCGCGGCGCA
GCGTGCGCCTGCGCTGGTTCGCGGACGAGCACCCGGTGGACGCCGCCTTCGTGACCAACAGCACCGTGGC
CGACGAGCTCGGGCGCCGCACGCGCGTCTCCGTGGTGAACGTGACGCGCGCGGACGTCCCGGGCCTCGCG
GCCGCGGACGCCGCGGACGCGCTCGCGCCGAGCCTGCGCTGCGAGGCCGTGTGGTACCGCGACAGCGTGG
CCTCGCAGCGCTTCTCCGAGGCCCTGCGCCCCCACGTCTACCACCCGGCGGCGGTCTCGGTGCGCTTCGT
CGAGGGCTTCGCCGTCTGCGACGGCCTCTGCGTGCCCCCGGAGGCGCGCCTCGCCTGGTCCGACCACGCC
GCCGACACCGTCTACCACCTCGGCGCCTGCGCGGAGCACCCCGGCCTGCTCAACGTGCGGAGCGCCCGCC
CGCTGTCGGACCTCGACGGGCCCGTCGACTACACCTGCCGCCTCGAGGGCCTGCCCTCGCAGCTGCCCGT
CTTCGAGGACACGCAGCGCTACGACGCCTCCCCCGCGTCCGTGAGCTGGCCCGTCGTGAGCAGCATGATC
GTCGTCATCGCCGGCATCGGGATCCTGGCCATCGTGCTGGTCATCATGGCGACGTGCGTCTACTACCGCC
GGGCGGGGCCGTGACGCCCCGCGCGTTCCCCCCCCACGTCGAATCAATAA

Protein Sequence : Show Sequence

>YP_068347.1 envelope glycoprotein C [Suid alphaherpesvirus 1]
MASLARAMLALLALYAAAIAAAPSTTTALDTTPNGGGGGNSSEGELSPSPPPTPAPASPEAGAVSTPPVP
PPSVSRRKPPRNNNRTRVHGDKATAHGRKRIVCRERLFSARVGDAVSFGCAVFPRAGETFEVRFYRRGRF
RSPDADPEYFDEPPRPELPRERLLFSSANASLAHADALAPVVVEGERATVANVSGEVSVRVAAADAETEG
VYTWRVLSANGTEVRSANVSLLLYSQPEFGLSAPPVLFGEPFRAVCVVRDYYPRRSVRLRWFADEHPVDA
AFVTNSTVADELGRRTRVSVVNVTRADVPGLAAADAADALAPSLRCEAVWYRDSVASQRFSEALRPHVYH
PAAVSVRFVEGFAVCDGLCVPPEARLAWSDHAADTVYHLGACAEHPGLLNVRSARPLSDLDGPVDYTCRL
EGLPSQLPVFEDTQRYDASPASVSWPVVSSMIVVIAGIGILAIVLVIMATCVYYRRAGP

Molecule Role : Protective antigen
Molecule Role Annotation : A trial vaccine containing pseudorabies virus (PRV) glycoprotein gC as the main component showed excellent protection against virulent virus infection in pigs (Katayama et al., 1997).
Related Vaccine(s): Pseduorabies Subunit Glycoprotein C Vaccine

14. UL50

Gene Name : UL50
Sequence Strain (Species/Organism) : Suid herpesvirus 1
NCBI Gene ID : 2952537
NCBI Protein GI : 51557490
Locus Tag : SuHV1_gp07
Genbank Accession : BK001744
Protein Accession : YP_068324
Taxonomy ID : 10345
Gene Starting Position : 8442
Gene Ending Position : 9457
Gene Strand (Orientation) : -
Protein Name : deoxyuridine triphosphatase
Protein pI : 9.02
Protein Weight : 26335.82
Protein Length : 268
Protein Note : 3.6.1.23

DNA Sequence : Show Sequence

>gi|51557483:8442-9457 Suid herpesvirus 1, complete genome
CTTTATTGAAAAATGAGAAGAGCGGGCCCCGCGTGTGTGCGCGCGCGGTCGCCACCGTCCGGGTCCGGCG
CGGGCCGCCGCGGCCTCACAGGCCCGTGGAGCCGAAGCCGCGGGCCCCGCGCGCGCTGCTCGGGGCGACA
AAGTCCCGCGCGAAGAGCCACGCGGGGCGGTGCTGCATCGGGGCGCGCGGGGTGGCGGGGAACGGGGACC
GTCCCGTGATCCAGCCCAGCGGCTCGCGCGTCAGCACCAGCTGGGCCACGCGCTGGCCCGACTCCAGGGT
CACCGGGTGCGCGCCGCGGTTCTGGATCCGGAAGCGGCACGGGCCGCTCTCCCAGGGCGTGGGGAAGACG
ACGATGCCGCGGAGGTTGAGGGACGAGCGCCCGAAGACGTAGGCCCAGTGGCGGCCGTCCGTGCGGTGCA
CCGGTAGCGTCACGGTCTCGGCGCCCCCGGGGGGCAGCACCAGCTCGCGCGGGCAGGGGATGTCGTATCC
GGCGTCCTCGTCGCGCTTCGGCGCAAAGACCTCGAAGAAGGGCACGTCCGTGGCGATGCCCGGCGCGAGG
CGCAGCGGCGTGCAATAAAATTGCGCCCGTGGGCGGCCCGGGGCTACAATCGCCTGCACCTCGCCGCGAA
AGCCCGCGTCCACGATCCCGTTGGTGGTGCGACCGCCCCCGCGCTGCGCGACGAGCATCGCGTATCCGGT
GGGCAGCGCCACCTTGAGGCCCAGGGGAACCTTATAAAATCCCTCCGGCCCCGGACTCCGGCACACCAGC
AGCCGCCCTCCGTCCACGGTCACGGGCTCGCTCGCACACACCAGGATGGTCTCCTCCGCCGGGCTCTCGC
TGACGCTCGTCGCCGCGCTCTGCGCGCTGGTGGCCCCCGCGCTCTCTTCCATCGTCTCCACCGAGGGGCC
GCTGCCGCTGCTGCGCGAGGAGTCGCGGATCAACTTCTGGAACGCCGCCTGCGCCGCCAGGGGAGTCCCG
GTGGATCAGCCCACGGCCGCCGCCGTGACATTTTAT

Protein Sequence : Show Sequence

>gi|51557490|ref|YP_068324.1| deoxyuridine triphosphatase [Suid herpesvirus 1]
MEESAGATSAQSAATSVSESPAEETILVCASEPVTVDGGRLLVCRSPGPEGFYKVPLGLKVALPTGYAML
VAQRGGGRTTNGIVDAGFRGEVQAIVAPGRPRAQFYCTPLRLAPGIATDVPFFEVFAPKRDEDAGYDIPC
PRELVLPPGGAETVTLPVHRTDGRHWAYVFGRSSLNLRGIVVFPTPWESGPCRFRIQNRGAHPVTLESGQ
RVAQLVLTREPLGWITGRSPFPATPRAPMQHRPAWLFARDFVAPSSARGARGFGSTGL

Molecule Role : Virmugen
Molecule Role Annotation : A UL50 mutant is attenuated in pseudorabies virus and induces significant protection in pigs from challenge with the highly virulent NIA-3 strain of PrV (Jöns et al., 1997).
Related Vaccine(s): Pseudorabies virus UL50 mutant vaccine

15. US3

Gene Name : US3
Sequence Strain (Species/Organism) : Suid herpesvirus 1
NCBI Gene ID : 2952561
NCBI Protein GI : 126802155
Locus Tag : SuHV1_gp65
Genbank Accession : BK001744
Protein Accession : YP_068384
Taxonomy ID : 10345
Gene Starting Position : 118238
Gene Ending Position : 120955
Gene Strand (Orientation) : +
Protein Name : serine/threonine protein kinase US3
Protein pI : 4.59
Protein Weight : 34398.44
Protein Length : 334
Protein Note : PK family

DNA Sequence : Show Sequence

>gi|51557483:118238-120955 Suid herpesvirus 1, complete genome
AAATAAAGGAGTCCGGGGAATTTGTTCCTTATACCTTGCCGGGCTCAGCAGGGGGTTGTCGCGCGTCCAC
GCCCAGCGCTCGCACGCAGCAACAATGGCCGACGCCGGAATCCCCGACGAGATCCTGTACTCGGACATCA
GCGACGACGAGATCATCATCGACGGCGACGGCGACAGCAGCGGGGACGAGGACGACGATGACGGGGGGCT
GACGCGGCAGGCCGCGGCGCGCATCGTCACGGACCTGGGCTTCGAGGTGCTGCAGCCCCTGCAGTCGGGC
TCGGAGGGCCGCGTCTTCGTGGCCCGCCGGCCGGGCGAGGCGGACACGGTGGTGCTGAAGGTGGGCCAGA
AGCCCTCGACGCTGATGGAGGGCATGCTGCTGCAGCGCCTGTCCCACGATAACGTCATGCGCATGAAACA
GATGCTCGCCCGGGGCCCGGCGACGTGCCTGGTCCTGCCGCACTTTCGGTGCGATCTGTACAGCTACCTG
ACCATGCGGGACGGGCCGCTGGACATGCGCGACGCCGGGTGCGTGATCCGGGCCGTGCTCCGCGGGCTCG
CCTACCTGCACGGGATGCGCATCATGCATCGCGACGTCAAGGCGGAGAACATCTTCCTCGAGGACGTGGA
CACGGTGTGCCTAGGGGACCTCGGGGCCGCGCGCTGCAACGTGGCGGCGCCCAACTTTTACGGGCTCGCC
GGGACCATCGAGACCAACGCCCCCGAGGTGCTCGCGCGCGACCGCTACGACACCAAGGTCGACGTCTGGG
GCGCGGGGGTGGTGCTCTTCGAGACGCTGGCCTACCCCAAGACGATCACCGGCGGGGACGAGCCCGCGAT
CAACGGGGAGATGCACCTGATCGACCTCATCCGCGCCCTCGGGGTGCACCCCGAGGAGTTCCCGCCCGAC
ACGCGCCTCCGGAGCGAGTTCGTCCGGTACGCCGGGACCCACCGCCAGCCGTACACGCAGTACGCGCGCG
TGGCTCGCCTCGGGCTGCCCGAGACGGGGGCTTTCCTGATTTACAAGATGTTGACGTTTGATCCCGTCCG
CCGCCCTTCCGCTGATGAGATACTCAACTTTGGAATGTGGACCGTATAAAACGGGCCGGCTCCGAGCGGT
AGGACACACACACCTTTGCGCATCTCCACAGCTCAACAATGAAGTGGGCAACGTGGATCCTCGCCCTCGG
GCTCCTCGTGGTCCGCACCGTCGTGGCCAGAGAGGCCCCTCGGGAGCTCTGCTACGGCCACCCCGTCCAC
GACGACCGGCGGCCCGTCGGGCCCGCGACCGACGCCCAGCCCGTGAACCCGCTCGCCCCCGCCAACGCCA
CCGGGACGGACTACTCTCGCGGCTGCGAGATGCGCCTCCTGGATCCGCCTCTCGACGTATCGTCCCGCTC
CTCGGACCCCGTCAACGTGACCGTCGCCTGGTTCTTTGACGGCGGCCACTGCAAGGTGCCCCTCGTCCAC
CGCGAGTACTACGGCTGCCCCGGGGACGCCATGCCCTCCGTCGAGACGTGCACCGGCGGGTACTCGTACA
CCCGCACGCGCATCGACACCCTGATGGAGTACGCCCTCGTGAACGCCAGCCTCGTGCTGCAGCCCGGGCT
GTACGACGCCGGCCTGTACATCGTCGTGCTCGTCTTTGGCGACGACGCCTACCTCGGCACCGTCTCCCTG
TCGGTGGAGGCCAACCTGGACTACCCCTGCGGCATGAAGCACGGGCTCACGATCACCCGCCCCGGGGCCA
CCCTCCCACCCATCGCCCCCACGGCCGGCGACCACCAGCGCTGGCGCGGGTGCTTCCCCTCGACCGACGA
GGGCGCCTGGGAGAACGTGACCGCCGCCGAGAAGGGCCTGTCCGACGACTACGCCGACTACTACGACGTG
CACATCTTCCGCTCGGAGTCTGACGACGAGGTCGTCCACGGCGATGCCCCCGAGGCCCCCGAGGGCGAGG
AGGTGACCGAGGAGGAGGCCGAGCTGACCTCCAGCGACCTCGACAACATCGAGATCGAGGTCGTGGGCTC
TCCCGCCGCTCCCGCCGAGGGCCCGGCGACGGAGGAGGGGCGCGGGGCCGAGGAGGACGAGGAGCTGACC
TCCAGCGACCTCGACAACATCGAGATCGAGGTCGTGGGCTCGCCGCGGCCGCCCGCTTCTTCGCCGCCTC
CACCACCCCCGCGCCCCCACCCGCGCGGCCGAGATCACGACCATGACCACGGTCACCACCGTGCGGACGA
CCGAGGACCCCAGCGGCATCACCGACTGCCGCCGGAGCCGACCTTCGTCTCGCCCTCTGACATCTTCGTG
ACCCCCACCGGCAGCCCCGCCCTGCTCCTGGGCTTCCTGGGCAGCGCGCTCGCCTCGCGCCCCCTGCACC
TGACGGCCGGGGAGACGGCCCAGCACGTGCGCGAGGCCCAGCAGAAGAGCCGCCACATCCGCTCCCTCGG
CGGCCTCCAGCTCTCGGTCGAGACCGAGACCACCAACACCACCACCACCCAGACGGGCCTGTCGGGCGAC
ATCCGCACCTCGATCTACATCTGCGTCGCCCTCGCCGGCCTGGTCGTCGTGGGCATCGTCATCATGTGCC
TCCATATGGCGATCATCAGGGCCCGGGCCCGGAACGACGGCTACCGCCACGTGGCCTCCGCCTGACCCGG
CCCCGCCCGACTCCCCCGCGATTCCCCCCCTCTCTCACCGGGTGTCCATCTTCAATAA

Protein Sequence : Show Sequence

>gi|126802155|ref|YP_068384.2| serine/threonine protein kinase US3 [Suid herpesvirus 1]
MADAGIPDEILYSDISDDEIIIDGDGDSSGDEDDDDGGLTRQAAARIVTDLGFEVLQPLQSGSEGRVFVA
RRPGEADTVVLKVGQKPSTLMEGMLLQRLSHDNVMRMKQMLARGPATCLVLPHFRCDLYSYLTMRDGPLD
MRDAGCVIRAVLRGLAYLHGMRIMHRDVKAENIFLEDVDTVCLGDLGAARCNVAAPNFYGLAGTIETNAP
EVLARDRYDTKVDVWGAGVVLFETLAYPKTITGGDEPAINGEMHLIDLIRALGVHPEEFPPDTRLRSEFV
RYAGTHRQPYTQYARVARLGLPETGAFLIYKMLTFDPVRRPSADEILNFGMWTV

Molecule Role : Virmugen
Molecule Role Annotation : Researchers intranasally inoculated pigs, the natural host of this virus, with mutant PRV strains in which the genes encoding the protein kinase (US3) proteins were inactivated. After challenge infection with the virulent PRV strain NIA-3, no virus was excreted by wt PRV- and PK- mutant-immunized animals, indicating complete protective immunity (Kimman et al., 1994).
Related Vaccine(s): Pseudorabies virus US3 mutant vaccine

16. US6

Gene Name : US6
Sequence Strain (Species/Organism) : Suid alphaherpesvirus 1
VO ID : VO_0011240
NCBI Gene ID : 2952521
NCBI Protein GI : 51557553
Locus Tag : SuHV1_gp68
Genbank Accession : BK001744
Protein Accession : YP_068387
Taxonomy ID : 10345
Gene Starting Position : 121023
Gene Ending Position : 123397
Gene Strand (Orientation) : +
Protein Name : envelope glycoprotein D
Protein pI : 9.38
Protein Weight : 41362.03
Protein Length : 400
Protein Note : envelope glycoprotein D; the Herpesviridae are non-segmented dsDNA viruses with genomes ranging from 120-230kbp; although herpes viruses vary greatly in sequence identity and homology, they all share four common elements: an envelope, a tegument which is composed of viral enzymes, a capsid of 162 capsomers, and a core composed of genomic DNA;virion envelope glycoproteins bind to cellular receptors; the nonessential glycoprotein gC interacts with cell surface proteoglycans, whereas the essential glycoprotein gD is involved in stable secondary attachment

DNA Sequence : Show Sequence

>NC_006151.1:121023-123397 Suid herpesvirus 1, complete genome
CCATAAAATTGGGTGGGGGCCCCAGGTTCCCATACACTCACCTGCCAGCGCCATGCTGCTCGCAGCGCTA
TTGGCGGCGCTGGTCGCCCGGACGACGCTCGGCGCGGACGTGGACGCCGTGCCCGCGCCGACCTTCCCCC
CGCCCGCGTACCCGTACACCGAGTCGTGGCAGCTGACGCTGACGACGGTCCCCTCGCCCTTCGTCGGCCC
CGCGGACGTCTACCACACGCGCCCGCTGGAGGACCCGTGCGGGGTGGTGGCGCTGATCTCCGACCCGCAG
GTGGACCGGCTGCTGAACGAGGCGGTGGCCCACCGGCGGCCCACGTACCGCGCCCACGTGGCCTGGTACC
GCATCGCGGACGGGTGCGCGCACCTGCTGTACTTTATCGAGTACGCCGACTGCGACCCCAGGCAGATCTT
TGGGCGCTGCCGGCGCCGCACCACGCCGATGTGGTGGACCCCGTCCGCGGACTACATGTTCCCCACGGAG
GACGAGCTGGGGCTGCTCATGGTGGCTCCGGGGCGGTTCAACGAGGGCCAGTACCGGCGCCTGGTGTCCG
TCGACGGCGTGAACATCCTCACCGACTTCATGGTGGCGCTCCCCGAGGGGCAAGAGTGCCCGTTCGCCCG
CGTGGACCAGCACCGCACGTACAAGTTCGGCGCGTGCTGGAACGACGAGAGCTTCAGGCGGGGCGTGGAC
GTGATGCGATTCCTGACGCCGTTCTACCAGCAGCCCCCGCACCGGGAGGTGGTGAACTACTGGTACCGCA
AGAACGGCCGGACGCTCCCGCGGGCCTACGCCGCCGCCACGCCGTACGCCATCGACCCCGCGCGGCCCTC
GGCGGGCTCGCCGAGGCCCAGGCCCCGGCCCCGGCCCCGGCCGAAGCCCGAGCCCGCCCCGGTGACGCCC
GCGCCCCCCGGCCGCCTGCCCGAGCCGGCGACGCGGGACCACGCCGCCGGGGGCCACCCCACGCCGCGAC
CCCCGAGGCCCGAGACGCCGCACCGCCCCTTCGCCCCGCCGGCCGTCGTGCCCAGCGGGTGGCCGCAGCC
CGCGGAGCCGTTCCAGCCGCGGACCCCCGCCGCGCCGGGCGTCTCGCGCCACCGCTCGGTGATCGTCGGC
ACGGGCACCGCGATGGGCGCGCTCCTGGTGGGCGTGTGCGTCTACATCTTCTTCCGCCTGAGGGGGGCGA
AGGGGTATCGCCTCCTGGGCGGTCCCGCGGACACCGACGAGCTAAAAGCGCAGCCCGGTCCGTAGCCTCC
GCAGTACCGGCGTCGATGATGATGGTGGCGCGCGACGTGACCCGGCTCCCCGCGGGGCTCCTCCTCGCCG
CCCTGACCCTGGCCGCCCTGACCCCGCGCGTCGGGGGCGTCCTCTTCAGGGGCGCCGGCGTCAGCGTGCA
CGTCGCCGGCAGCGCCGTCCTCGTGCCCGGCGACGCGCCCAACCTGACGATCGACGGGACGCTGCTGTTT
CTGGAGGGGCCCTCGCCGAGCAACTACAGCGGGCGCGTGGAGCTGCTGCGCCTCGACCCCAAGCGCGCCT
GCTACACGCGCGAGTACGCCGCCGAGTACGACCTCTGCCCCCGCGTGCACCACGAGGCCTTCCGCGGCTG
TCTGCGCAAGCGCGAGCCGCTCGCCCGGCGCGCGTCCGCCGCGGTGGAGGCGCGCCGGCTGCTGTTCGTC
TCGCGCCCGGCCCCGCCGGACGCGGGGTCGTACGTGCTGCGGGTCCGCGTGAACGGGACCACGGACCTCT
TTGTGCTGACGGCCCTGGTGCCGCCCAGGGGGCGCCCCCACCACCCCACGCCGTCGTCCGCGGACGAGTG
CCGGCCCGTCGTCGGATCGTGGCACGACAGCCTGCGCGTCGTGGACCCCGCCGAGGACGCCGTGTTCACC
ACGCCGCCCCCGATCGAGCCAGAGCCGCCGACGACCCCCGCGCCCCCCCGGGGGACCGGCGCCACCCCCG
AGCCCCGCTCCGACGAAGAGGAGGAGGACGAGGAGGGGGCGACGACGGCGATGACCCCGGTGCCCGGGAC
CCTGGACGCGAACGGCACGATGGTGCTGAACGCCAGCGTCGTGTCGCGCGTCCTGCTCGCCGCCGCCAAC
GCCACGGCGGGCGCCCGGGGCCCCGGGAAGATAGCCATGGTGCTGGGGCCCACGATCGTCGTCCTCCTGA
TCTTCTTGGGCGGGGTCGCCTGCGCGGCCCGGCGCTGCGCGCGGAATCGCATCTACCGGCCGCGACCCGG
GCGCGGCCCGGCGGTCCACGCGCCGCCCCCGCGGCGCCCGCCCCCCAGCCCCGTCGCCGGGGCGCCCGTC
CCCCAGCCCAAGATGACGTTGGCCGAGCTTCGCCAGAAGCTGGCCACCATCGCAGAGGAACAATA

Protein Sequence : Show Sequence

>YP_068387.1 envelope glycoprotein D [Suid alphaherpesvirus 1]
MLLAALLAALVARTTLGADVDAVPAPTFPPPAYPYTESWQLTLTTVPSPFVGPADVYHTRPLEDPCGVVA
LISDPQVDRLLNEAVAHRRPTYRAHVAWYRIADGCAHLLYFIEYADCDPRQIFGRCRRRTTPMWWTPSAD
YMFPTEDELGLLMVAPGRFNEGQYRRLVSVDGVNILTDFMVALPEGQECPFARVDQHRTYKFGACWNDES
FRRGVDVMRFLTPFYQQPPHREVVNYWYRKNGRTLPRAYAAATPYAIDPARPSAGSPRPRPRPRPRPKPE
PAPVTPAPPGRLPEPATRDHAAGGHPTPRPPRPETPHRPFAPPAVVPSGWPQPAEPFQPRTPAAPGVSRH
RSVIVGTGTAMGALLVGVCVYIFFRLRGAKGYRLLGGPADTDELKAQPGP

Molecule Role : Protective antigen
Molecule Role Annotation : The pseudorabies virus (PrV) DNA vaccine carrying the glycoprotein D (gD) gene delivered by E. coli was able to induce protective immune responses in mice against a lethal PrV challenge (Shiau et al., 2001). Immunization of mice with affinity-purified gD of Aujeszky's disease virus (ADV, Suid herpesvirus 1) induced a strong humoral immune response and protected mice against lethal ADV challenge (Vrublevskaia et al., 2007).

III. Vaccine Related Host Genes

1. Ifng (Interferon gamma)

Gene Name : Ifng (Interferon gamma)
Sequence Strain (Species/Organism) : Mouse
NCBI Gene ID : 15978
NCBI Protein GI : 33468859
Genbank Accession : NM_008337
Protein Accession : NP_032363.1
Other Database IDs : MGI:107656; UniProt: P01580
Taxonomy ID : 10090
Gene Strand (Orientation) : ?

DNA Sequence : Show Sequence

>gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
AGTATACCTTTGCAACC

Protein Sequence : Show Sequence

>gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
LLPESSLRKRKRSRC

Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
Additional Molecule Role : Vaximmutor
Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
Related Vaccine(s): Pseudorabies Subunit gB Protein Vaccine

2. Il2

Gene Name : Il2
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16183
NCBI Protein GI : 7110653
Locus Tag : DN-144H19.3
Genbank Accession : AF195954
Protein Accession : NP_032392
Taxonomy ID : 10090
Chromosome No : 3
Gene Starting Position : 37120712
Gene Ending Position : 37125953
Gene Strand (Orientation) : -
Protein Name : interleukin 2
Protein pI : 4.63
Protein Weight : 18506.61
Protein Length : 169
Protein Note : Also known as Il-2

DNA Sequence : Show Sequence

>gi|372099107:37120712-37125953 Mus musculus strain C57BL/6J chromosome 3, GRCm38 C57BL/6J
ATTTTTTTTTAGAGGAGAGCTTTATTTCTTGAAAACACTGATTAACATAGAGTTCACAGGAATAACTGAG
AAGTTATTTTAGCGCTTACTTTGTGCTGTCCTAAAAATGACAGACATCTGAGCTTATTTATATTTGAATC
ATCTAAATACTTTTATTAATGAGTCTACCTACACATGATATTTAACAATTCAATATAATAAATAATTTCA
GATAAATAGTTTAAAACATTTTTGAGCCCTTGGGGCTTACAAAAAGAATCTTTAAAGATCCATATTTATC
ATCTGAAGACTAGTAGTTACAAAAGATAGTAAACAATACATCCAAAAATAAATTAAAGTTAAATATTTAA
ATAAATAGAGAGCCTTATGTGTTGTAAGCAGGAGGTACATAGTTATTGAGGGCTTGTTGAGATGATGCTT
TGACAGAAGGCTATCCATCTCCTCAGAAAGTCCACCACAGTTGCTGACTCATCATCGAATTGGCACTCAA
ATGTGTTGTCAGAGCCCTAAAAAAGAATATAAAATTGTAGGCTAAGGTAGCTTACTTTGCATATAATTAT
TCTTCACGGAAGCTTTTAGGCATTTATTTTAACACTGGTTAAATATATTGAACATTCACATTTCTGAAAT
GATCACAATTATTGGGTTATGTTTTATTATGATATCAAATGGTAAAGCATAAAAAATAAAGCTCTCCAGA
GTTGATTACCAACGAGCATTTGATGGGAACACTGAACACTTCTGTAGAATTCAGTTGCTTGACCACTTTT
TCTCTGGTAAGTGTACACAGCTATGCATCAATTTAGGCTTCAGGAATGGAGAAATGATACTTGTTATATG
TTCTTTGCTAAATCTTAAGAGTCTATTATTCAATTCTTTCCCCCCCAAGTCAGAATGGGAACAACCCAGA
GTAGGTTAGGACAAGCATCTATTGGGGGAAATTTGGAGAATTTTGTTATAGATATACCTACCCTGATACC
AGTGTAGATGAATCACAAAGTTTGTACATGTATAAATATTATAAATTTGTTCTGTGGATTAGCTTTTTGA
AAGCTAACCCTCTTTGCTAAAGGAGATATTTTAATTCTGTTTTTAGGTGACAAGCTGAGAAGGAAATATT
TTGACAAATTTATGGGATGGCTTTTCCAAATCTGACTAATTCCTTGTAAGTCAAATGAGGCAGATTATTC
CTTTTAAATAAATACTCCTTTTCAAATAAGCTGCTGTGGTCTTATATACCCTCTTTACATAGCTACTTCA
ATATTCTAATGTCTTGAATTTTTCATTTTTAAAAATATGTGGCCCTTGAAACATTTTTCTAGTTCTCATG
TAGCTCTAAAGTTTGTACCATTCATTAGGGGTGAACTTTCTGATGTTGGCCATAGATTTTAGAGCATCCA
TCTTTGCATACGTAAGTACTCGATTTTAGAGCATCCATCTTAGCATACGTAAGTACTCACACCTTTCAAC
GTCTAGATGATGTGTATTTCAGCCTGCTTTAAAAACCTCAAATGGAAGCTGGGCAGTGGTGGTGCAGGCC
TTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGT
GAGTTCCAGGACAGCCAGGGCTACACAGAGAAATCCTGTCTCAAAAAAACAAACAAACAAACAAACAAGA
AACAAACAAACAAAAAAAACAAAAACAAACAAAACAAAACCTCAAATGGAAACAGTGCACTGAAATGTGA
CTTTGGTTTGGAAAGGACTAGCCCACACCCTCTTGGAGGCTTGCTGCTACTGTCTCACCAGAGTCCATGA
TTCTTGTGCATTTAAAAACAAAGCTCTGCAGTGGGCTCTCCCCGTCCTGCTCTGTCCATTTTAATGGCTG
CCATTTTTGGAGAGAAATGTCTGTTTTTCTACCAATACCAGCACAACTTCTCTGGAAAAACTTTTCAGAT
AATTTTTTCTGATCTGATGAATGTAACACCAGCAAGAGTTGCTTGTTTCTTGTGGAATTCTACTCCGTGC
TTTCTCTCACATCCAGTTCTATGCTGGTGTGGAGGGAGCAGAGTGTTCATGTTCCCAGTTTCCTTGCAGG
TGATGGTAGGTGGAAATTCTAGCATCATCCTACAGTGGAAGGATTCACTTGCACAGTGACTTTAAACTTT
GGCTGACTAAATGCACAGAACCCATCAAAGACCAGAAATGGCAAGCCACTTAAAAATGCATTTCTTCTCT
ATTTTATTTCCAGATTAGCAAATAAAGCAACACCTTACCTTTAGTTTTACAACAGTTACTCTGATATTGC
TGATGAAATTCTCAGCATCTTCCAATTGAAAGCTTTTGCTTTGAGTCAAATCCAGAACATGCCGCAGAGG
TCCAAGTTCATCTTCTAGGCACTGAAGATCTTTCAATTCTGTGGCCTAGAGGAGTAATAAGCTTAACCAT
CAGCTCAGCTCACCACATACTGAAGAGCCAGAAAGTTAGTCTGCAGTCTCTCTAATCAAGAGAAGGCAGC
ACATAGCTTTTACCACTCCCTGTCCTCTGTAAAGGGGGACTGAGGTAATCGATGCCATAAATCTAGAAGA
GCAAGAGCTCACAACCATAAGGGTCACCTTGACTGTTAGGCCACTCTAGTGAGCTCTTCTGGCTTCATTA
GACTTTGTAAAAAGTCTGTGTTTCTCTACCAATGCATAGCACAAGTTCAGACTATTGTTCCAATCTACAA
AGAAATCTGCAAGGTTCACATTCTAATATCTAATCGCAGAGTTGAGAATCACAGAAGGGTAAGGGACAGG
AATCCTTGGATGCCAACTGTCATAGGCCTAAATCTTACAGATTAGGTTATCATGGCCCTGACTGAGGGGT
GTCAAGATAGCCAGGAAGACACATGTAATATTTTTTAAATTTATACTTCTCTGCAATTACTAAAGATGAG
TTTTTTAAAAACCCATATGTCAAGCTAGGAAGATGGCTTTTAAGGTAAAGGTACCTGCCACCAAACTTGA
TGATTTGAGTTCAATTGCAGTGACCCACCCACATTGTGGAAAGAATGGCCCAACTTTCATATTTCGTCTT
CTGACCTCCACAGTTACACTGTGATACACATGCACCTCCACACATACATACATACATACATACATACATA
CATACATACATACATACATACATACATAAAATGTAAAATCTATTTGTCCCAATGAAAAAAGTCATAACAT
TTTGGCAGAACACTTGGGAGAATTTTTCTGATCCATGTATGAAATAATGTTTAAATACATCATGCATACT
TACATACATATACAGGGAAAAAGGATATTGTCTAGCTCTATAGTACACTATGATTTCCATAAAGTCAGCT
GCTTTAAGTCTACAGTGAACTGTGTTATCTCCATTTTACCAAGTCTCACAATCAGTGTCTTTCCCACAAC
CACATGAACGGGGAGCAAGGGTGGCCCGGTCGGTCGGTCGCTCCTTTCATTAACCACAGCCTTTGAATTA
CATGCTTCTATTGTTTTTAAAATCTTACATACCGAATGCAGGTTAAATGAAAACATCAGCAGAATTTACC
ACATTACTCTTGTTTTCAATAATTATTTTAAAACTTAGATTTCTAGGAATTTCACCACTCTCTAAATTAA
CATGATCTCTGTTGTTTCTCTAACAATCCTTAGAGGAAGAGGTTTGACAAGATAACACGTAGTTCCTGAT
TGGTCTGCTATTGACCTTCCAACTTGTTATGGTGTGTCTTGGGTATTCAGTGTGGAGCTCCTTCCCTCAC
AGAACTCTCCAGTTTGTTACTGTGGGAGCTGAACATTATGTGACTGTAATTAAGCTGGAAGAGCAACTGG
GGCCTTTCTTTTTCATCTCCTGTAGAACAGCTAGAGGCAAAGTTCCCCTGACTCAATAGGAATGGGTCAA
CAGCTGCCTCTTTGTCCCCTTAGATCCCATTTTGCTCTAGGCTTGGATTTACTTGGGACAAGCTCTTTCT
AGGACCAAAGTTATCCATTCTCTTTTGACTTTCGGGACTAGAGCAAATTTCTACCCTTGCTGGAGTTCTA
AGAGCTTGTGTAAGCTGTTTTGTATCACACAACATTCTGCCTCCAAAGGAGACCAAAAAACCTTTCAATA
TTGAGAGATAGTATCATGTAGTAATATTACAGCTCTCTAGTTCCACTAGTTAAAAGAGACTGTATAAATC
CAAAATTGTTTTATACATCTCGGACTTCTTCTCTAGCACTAATAGTGTGTTTATGTGCTGTTGGCCAGAC
ACTGTTCTGAAACCTGACAAGTGATAAGCATAGCAATGATCATTTATGTAAGTGGGTGAATGAGCATAGA
CAACTTCCTGAGGCCATGAGAGCTAGGCCCTAGCAGGGCTGGACTCCACTGCAGATCATTGGCCCTAGAA
TCTTAACATTGGTTATGCTGCTCCTCAAAAATGCAGTCATAGGAATCATTCTTAAAACAGTAAAGTGTGT
GTAAAATGAGACCCTCTCAAGTCAATTTTTACATTTAAGAAGCTGAAATAATGCACCTCTCTCAAATATC
TGTAGGACAAGCATAAAACAGAAATCAACAGTCTTTAAATTATCCCAGCATCAAAATGCAATCATCTTGC
ATTTTCTTCTCATTCCCTTTCCCAACACATAAATACACAGGAAAAATGTAAAGAAAACCATTTAAAAGAA
GTACCATTGTCAAGATTTTTTTACAGAGAGATATCAGAGAAACTTATTTTCTTCAGATGAGGTACTATTC
ATCTGGTCACATGTTATTTACACGTTATACACATGTATGTTACTTTGAATTCATTAGAGCACCAGTTAAA
CACAGAAACTCACTCACCTGCTTGGGCAAGTAAAATTTGAAGGTGAGCATCCTGGGGAGTTTCAGGTTCC
TGTAATTCTGAGAAAGCGTAACACATTATTATTAAAGGTTATTAAACACAGCCTTTGGCAAGAAAGCTAA
AGGTATTGCCTATAGATGGGATGGCTGTGCACTTACCTCCATCCTGCTCAGGAGCTCCTGTAGGTCCATC
AACAGCTGCTCCAGGTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGTGCTTCCGCTGTAGAGC
TTGAAGTGGAGCTTGAAGTGGGTGCGCTGTTGACAAGGAGCACAAGTGTCAATGTGACACAGGATGCGAG
CTGCATGCTGTACATGCCTGCAGGACTTGAGGTCACTGTGAGGAGTGATTAGCAAGGGTGAT

Protein Sequence : Show Sequence

>gi|7110653|ref|NP_032392.1| interleukin-2 precursor [Mus musculus]
MYSMQLASCVTLTLVLLVNSAPTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKL
PRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFEC
QFDDESATVVDFLRRWIAFCQSIISTSPQ

Molecule Role : Vaximmutor
Related Vaccine(s): Pseudorabies Subunit gB Protein Vaccine

3. Il4 (interleukin 4)

Gene Name : Il4 (interleukin 4)
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16189
NCBI Protein GI : 10946584
Locus Tag : RP23-188H3.4
Genbank Accession : NM_021283.1
Protein Accession : NM_021283.1
Taxonomy ID : 10090
Gene Strand (Orientation) : ?
Protein Name : IL-4, Interleukin 4

DNA Sequence : Show Sequence

>gi|10946583|ref|NM_021283.1| Mus musculus interleukin 4 (Il4), mRNA
GGATCCCCGGGCAGAGCTGGGGGGGGATTTGTTAGCATCTCTTGATAAACTTAATTGTCTCTCGTCACTG
ACGGCACAGAGCTATTGATGGGTCTCAACCCCCAGCTAGTTGTCATCCTGCTCTTCTTTCTCGAATGTAC
CAGGAGCCATATCCACGGATGCGACAAAAATCACTTGAGAGAGATCATCGGCATTTTGAACGAGGTCACA
GGAGAAGGGACGCCATGCACGGAGATGGATGTGCCAAACGTCCTCACAGCAACGAAGAACACCACAGAGA
GTGAGCTCGTCTGTAGGGCTTCCAAGGTGCTTCGCATATTTTATTTAAAACATGGGAAAACTCCATGCTT
GAAGAAGAACTCTAGTGTTCTCATGGAGCTGCAGAGACTCTTTCGGGCTTTTCGATGCCTGGATTCATCG
ATAAGCTGCACCATGAATGAGTCCAAGTCCACATCACTGAAAGACTTCCTGGAAAGCCTAAAGAGCATCA
TGCAAATGGATTACTCGTAGTACTGAGCCACCATGCTTTAACTTATGAATTTTTAATGGTTTTATTTTAA
TATTTATATATTTATAATTCATAAAATAAAATATTTGTATAATGT

Protein Sequence : Show Sequence

>gi|10946584|ref|NP_067258.1| interleukin 4 [Mus musculus]
MGLNPQLVVILLFFLECTRSHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCR
ASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS

Molecule Role : Vaximmutor
Molecule Role Annotation : IL-4 plays an important role in Th2 immune response.
Related Vaccine(s): Pseudorabies Subunit gB Protein Vaccine

IV. Vaccine Information

1. NYVAC-PRV- gII/ gp50

a. Vaccine Ontology ID:

VO_0004778

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Gene Engineering of GII

Type: Recombinant vector construction
Description: A NYVAC vaccinia vector containing genes for pseudorables virus glycoproteins gII and gp50 (Brockmeier and Mengeling, 1996).
Detailed Gene Information: Click here.

f. Gene Engineering of gp50

Type: Recombinant vector construction
Description: A NYVAC vaccinia vector containing genes for pseudorables virus glycoproteins gII and gp50 (Brockmeier and Mengeling, 1996).
Detailed Gene Information: Click here.

g. Preparation

(Brockmeier and Mengeling, 1996) A NYVAC vaccinia vector containing genes for PRV gII and gp50 .

h. Immunization Route

Intramuscular injection (i.m.)

i. Pig Response

Vaccination Protocol: The pigs were vaccinated via intramuscular injection in the thigh with 10⁷ CCID50 of NYVAC/gp50 or NYVAC/gpII,gp50, 4 weeks apart (Brockmeier and Mengeling, 1996).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: Vaccinated pigs were challenged with 10⁸ PFU of Indiana-Funkhauser strain of PRV oronasally, 4 weeks after the second vaccination (Brockmeier and Mengeling, 1996).
Efficacy: Both NYVAC vectors protected pigs similarly from virulent pseudorabies virus challenge (Brockmeier and Mengeling, 1996).

2. Pseduorabies Subunit Glycoprotein C Vaccine

a. Vaccine Ontology ID:

VO_0004155

b. Type:

Subunit vaccine

c. Status:

Research

d. Gene Engineering of UL44

Type: Recombinant protein preparation
Description: Psuedorabies glycoprotein C was purified from pseudorabies infected cell lysates (Katayama et al., 1997).
Detailed Gene Information: Click here.

e. Immunization Route

Intramuscular injection (i.m.)

f. Pig Response

Vaccination Protocol: Pigs of each group were immunized intramuscularly with one ml of the different trial vaccines at two week intervals. Control pigs were vaccinated with unifected cell lysate antigens (Katayama et al., 1997).
Challenge Protocol: One week after immunization pigs were challenged intranasally with 10⁶ TCID50 of PRV Yamagata S-81 strain (Katayama et al., 1997).
Efficacy: Immunized pigs had a high (87.5%) rate of survival against lethal PRV challenge (Katayama et al., 1997).

3. Pseudorabies Modified Live Virus Vaccine (USDA: 1891.20)

a. Manufacturer:

Wyeth, Boehringer Ingelheim Vetmedica, Inc., Pfizer, Inc.

b. Vaccine Ontology ID:

VO_0001806

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Pig

4. Pseudorabies Modified Live Virus Vaccine (USDA: 1891.22)

a. Manufacturer:

Pfizer, Inc.

b. Vaccine Ontology ID:

VO_0001807

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Pig

5. Pseudorabies Modified Live Virus Vaccine (USDA: 1891.R2)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0001808

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Pig

6. Pseudorabies Modified Live Virus Vaccine (USDA: 1891.R4)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0001809

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Pig

7. Pseudorabies recombinant vector vaccine encoding gp50 and gII protein

a. Vaccine Ontology ID:

VO_0004156

b. Type:

Recombinant vector vaccine

c. Status:

Licensed

d. Gene Engineering of gp50

Type: Recombinant vector construction
Description:
Detailed Gene Information: Click here.

e. Gene Engineering of GII

Type: Recombinant vector construction
Description:
Detailed Gene Information: Click here.

f. Vector:

NYVAC vaccinia vector (Brockmeier and Mengeling, 1996).

g. Immunization Route

Intramuscular injection (i.m.)

h. Pig Response

Vaccination Protocol: 4-6 wk old male pigs from a PRV negative herd were vaccinated intramuscularly with two injections in the thigh of 10⁷ CCID50 of NYVAC/gp50gpII 4 weeks apart. The control group was not vaccinated (Brockmeier and Mengeling, 1996).
Challenge Protocol: Challenge consisted of administering 10⁸ PFU of Indiana-Funkhauser strain of PRV oranasally, 4 weeks after the 2nd immunization (Brockmeier and Mengeling, 1996).
Efficacy: Vaccinated pigs were protected from PRV challenge (Brockmeier and Mengeling, 1996).

8. Pseudorabies recombinant vector vaccine encoding gp50 protein

a. Vaccine Ontology ID:

VO_0004003

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Gene Engineering of gp50

Type: Recombinant vector construction
Description:
Detailed Gene Information: Click here.

e. Vector:

NYVAC vaccinia vector (Brockmeier and Mengeling, 1996).

f. Immunization Route

Intramuscular injection (i.m.)

g. Pig Response

Vaccination Protocol: 4-6 wk old male pigs from a PRV negative herd were vaccinated intramuscularly with two injections in the thigh of 10⁷ CCID50 of NYVAC/gpSO 4 weeks apart. The control group was not vaccinated (Brockmeier and Mengeling, 1996).
Challenge Protocol: Challenge consisted of administering 10⁸ PFU of Indiana-Funkhauser strain of PRV oranasally, 4 weeks after the 2nd immunization (Brockmeier and Mengeling, 1996).
Efficacy: Vaccinated pigs were protected from PRV challenge (Brockmeier and Mengeling, 1996).

9. Pseudorabies Subunit gB Protein Vaccine

a. Vaccine Ontology ID:

VO_0004041

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Gene Engineering of gB

Type: Recombinant vector construction
Description:
Detailed Gene Information: Click here.

e. Vector:

Replication-incompetent adenovirus (Han et al., 2008).

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Host Strain: C57BL/6
Vaccination Protocol: Groups of female mice were immunized with replication-incompetent adenoviruses expressing PrV glycoprotein (rAd-gB) by either the intranasal (i.n.) or intramuscular (i.m.) route. For i.m. administration, recombinant adenoviruses (10⁶ pfu/mouse) were injected into the anterior tibialis muscle three times at weekly intervals (0, 7, and 14 days). The i.n. immunizations were also performed three times at weekly intervals (0, 7, and 14 days) by depositing 10⁶ pfu of recombinant adenovirus onto the nares of deeply anesthetized mice. Control mice were immunized with replication-incompetent adenovirus expressing the LacZ gene (rAd-LacZ) (Han et al., 2008).
Challenge Protocol: The immunized mice were infected i.n. with the virulent PrV YS strain (10 LD50) two weeks after the final immunization. The challenged mice were examined daily to quantify the number of dead animals. Mice generally began to exhibit clinical signs of illness 3 to 4 days post-challenge (Han et al., 2008).
Efficacy: Recombinant adenovirus expressing gB (rAd-gB) was found to induce effective protective immunity against a virulent viral infection, regardless of whether it was administered via the muscular or systemic route (Han et al., 2008).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: In splenocytes and popliteal lymph node cells, IFN-gamma was induced to a significantly higher level in immunized animals as compared to replication-incompetent adenovirus expressing LacZ (rAd-LacZ) vaccinated mice. These elevated levels were detected 2 weeks after the final immunization
- Detailed Gene Information: Click here.
Host Gene Response of Il2
- Gene Response: In splenocytes and popliteal lymph node cells, IL-2 was induced to a significantly higher level in immunized animals as compared to replication-incompetent adenovirus expressing LacZ (rAd-LacZ) vaccinated mice. These elevated levels were detected 2 weeks after the final immunization (Han et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of Il4 (interleukin 4)
- Gene Response: In splenocytes and popliteal lymph node cells, IL-4 was induced to a significantly higher level in immunized animals as compared to replication-incompetent adenovirus expressing LacZ (rAd-LacZ) vaccinated mice. These elevated levels were detected 2 weeks after the final immunization (Han et al., 2008).
- Detailed Gene Information: Click here.

10. Pseudorabies Subunit gI Protein Vaccine

a. Vaccine Ontology ID:

VO_0004040

b. Type:

Subunit vaccine

c. Status:

Research

d. Gene Engineering of GI

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

e. Immunization Route

Intramuscular injection (i.m.)

f. Mouse Response

Host Strain: C57BL/10
Vaccination Protocol: For active immunization mice were injected i.m. (hind leg) with different concentrations of emulsified purified (by electroelution) fusion protein (Fuchs et al., 1990).
Challenge Protocol: Twenty-four h later the animals were infected intramuscularly (i.m.) into a hind leg using 0-1 ml of a lethal dose ( > 100 LD50) of strain Phylaxia, and the mice were observed for at least 10 days (Fuchs et al., 1990).
Efficacy: Vaccination of mice with fusion proteins containing the N terminus of gI from Suid herpesvirus 1 are able to confer protection to mice against a lethal challenge of PRV (Fuchs et al., 1990).

11. Pseudorabies virus glycoprotein E/G/UL23 mutant vaccine

a. Type:

Live, attenuated vaccine

b. Status:

Research

c. Host Species as Laboratory Animal Model:

Raccoon

d. Gene Engineering of glycoprotein G

Type: Gene mutation
Description: This glycoprotein E/G/UL23 mutant is from Suid herpesvirus 1 (Weigel et al., 2003).
Detailed Gene Information: Click here.

e. Gene Engineering of glycoprotein E

Type: Gene mutation
Description: This glycoprotein E/G/UL23 mutant is from Suid herpesvirus 1 (Weigel et al., 2003).
Detailed Gene Information: Click here.

f. Gene Engineering of UL23

Type: Gene mutation
Description: This glycoprotein E/G/UL23 mutant is from Suid herpesvirus 1 (Weigel et al., 2003).
Detailed Gene Information: Click here.

g. Immunization Route

intranasal immunization

h. Raccoon Response

Persistence: A glycoprotein E/G/UL23 is attenuated in raccoons (Weigel et al., 2003).
Efficacy: A glycoprotein E/G/UL23 mutant provides some protection in raccoons from challenge with wild type Pseudorabies virus (Weigel et al., 2003).

12. Pseudorabies virus gp50/gp63 mutant vaccine

a. Type:

Live, attenuated vaccine

b. Status:

Research

c. Host Species as Laboratory Animal Model:

Pig

d. Gene Engineering of gp50

Type: Gene mutation
Description: This gp50/gp63 mutant is from Suid herpesvirus 1 (Peeters et al., 1994).
Detailed Gene Information: Click here.

e. Gene Engineering of gp63

Type: Gene mutation
Description: This gp50/gp63 mutant is from Suid herpesvirus 1 (Peeters et al., 1994).
Detailed Gene Information: Click here.

f. Immunization Route

Intramuscular injection (i.m.)

g. Pig Response

Persistence: A gp50/gp63 mutant is attenuated in pigs (Peeters et al., 1994).
Efficacy: A gp50/gp63 mutant vaccine induces protection in pigs from challenge with wild type Pseudorabies virus (Peeters et al., 1994).

13. Pseudorabies virus UL50 mutant vaccine

a. Vaccine Ontology ID:

VO_0002989

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Pig

e. Gene Engineering of UL50

Type: Gene mutation
Description: This UL50 mutant is from Suid herpesvirus 1 (Jöns et al., 1997).
Detailed Gene Information: Click here.

f. Immunization Route

intranasal immunization

g. Pig Response

Persistence: A UL50 mutant is attenuated in pigs (Jöns et al., 1997).
Efficacy: A UL50 mutant induces protection in pigs from challenge with wild type Pseudorabies virus (Jöns et al., 1997).

14. Pseudorabies virus US3 mutant vaccine

a. Vaccine Ontology ID:

VO_0002990

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Pig

e. Gene Engineering of US3

Type: Gene mutation
Description: This US3 mutant is from Suid herpesvirus 1 (Kimman et al., 1994).
Detailed Gene Information: Click here.

f. Immunization Route

intranasal immunization

g. Pig Response

Persistence: A US3 mutant is attenuated in pigs (Kimman et al., 1994).
Efficacy: A US3 mutant induces complete protection in pigs from challenge with wild type Pseudorabies virus (Kimman et al., 1994).

15. Psuedorabies DNA vaccine encoding IE180 protein

a. Vaccine Ontology ID:

VO_0004039

b. Type:

DNA vaccine

c. Status:

Research

d. Gene Engineering of IE180

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

e. Vector:

pcDNA3.1 (Chang et al., 1998)

f. Immunization Route

Gene Gun

g. Mouse Response

Host Strain: BALB/c, C3H/HeJ, and C57BL/6
Vaccination Protocol: Two shots of DNA/mouse (1 /Ag/shot) were delivered with helium at 200 psi via the Helios Gene Gun to the shaven abdominal skin of 10 male mice of each strain, BALB/c, C57BL/6, C3H/HeJ. The mice received a booster once 14 days later with the same dose of DNA. In some experiments, pregnant mice of each strain were vaccinated immediately before mating and a booster was given 2 weeks later (Chang et al., 1998).
Challenge Protocol: Six months after second vaccination, groups of pcDNAIE180 immunized mice were challenged intraperitoneally with a precalibrated mouse acute lethal dose of wild-type PrV-Ka TK+ (15 plaque-forming units (PFU)/g of body weight). The challenged mice were observed three times daily for clinical signs, and percent survival was assessed within 4 to 5 days postchallenge (Chang et al., 1998).
Efficacy: Seven months after immunization with pcDNAIE180, an overall 25% of BALB/c, C3H/HeJ, and C57BL/6 mice receiving a lethal PrV challenge were protected (Chang et al., 1998).

16. Psuedorabies virus glycoprotein M mutant vaccine

a. Vaccine Ontology ID:

VO_0002985

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Pig

e. Gene Engineering of glycoprotein M

Type: Gene mutation
Description: This glycoprotein M mutant is from Pseudorabies virus (Dijkstra et al., 1997).
Detailed Gene Information: Click here.

f. Immunization Route

intranasal immunization

g. Pig Response

Persistence: A glycoprotein M mutant is attenuated in piglets (Dijkstra et al., 1997).
Efficacy: A glycoprotein M mutant induces protection in piglets from challenge with wild type Pseudorabies virus (Dijkstra et al., 1997).

17. rORFV-PRV-gC/gD

a. Vaccine Ontology ID:

VO_0004770

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Preparation

Based on the attenuated ORFV strain D1701-V, recombinants were produced that express the glycoproteins gC (D1701-VrVgC) or gD (D1701-VrVgD) of the alphaherpesvirus of swine, pseudorabies virus (PRV) (Fischer et al., 2003).

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Vaccination Protocol: Mice were injected intramuscularly (i.m.) with 107 TCID50s of the ORFV recombinants expressing the PRV glycoproteins in a total volume of 0.2 ml (0.1 ml for each hind leg). Immunization was repeated at 2-week intervals (Fischer et al., 2003).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: Mice were challenge infected intraperitoneally (i.p.) with 10² PFU of the highly virulent PRV strain NIA-3, and the C57BL/6 and 129/Sv/Ev mice were infected with 10³ PFU
Efficacy: Single or combined immunization with the ORFV recombinants protected different mouse strains of a host species nonpermissive for ORFV against a fulminant, lethal PRV challenge infection equal to immunization with PRV live vaccine. Most notably, even a single immunization with D1701-VrVgC was protective, whereas two applications of D1701-VrVgD were required for immune protection (Fischer et al., 2003).

18. Suvaxyn Aujeszky

a. Tradename:

Suvaxyn Aujeszky

b. Manufacturer:

Fort Dodge

c. Vaccine Ontology ID:

VO_0000936

d. Type:

Inactivated or "killed" vaccine

e. Status:

Licensed

f. Host Species for Licensed Use:

Pig

g. Immunization Route

Intramuscular injection (i.m.)

h. Description

gE- and thymidine kinase-deleted marker vaccine(Ferrari et al., 2000)

V. References

1. Brockmeier and Mengeling, 1996: Brockmeier SL, Mengeling WL. Comparison of the protective response induced by NYVAC vaccinia recombinants expressing either gp50 or gII and gp50 of pseudorabies virus. Canadian journal of veterinary research = Revue canadienne de recherche veterinaire. 1996; 60(4); 315-317. [PubMed: 8904669].

2. Chang et al., 1998: Chang SW, Bu J, Rompato G, Garmendia AE. A vector DNA vaccine encoding pseudorabies virus immediate early protein demonstrates partial protection in mice against lethal virus challenge. Viral immunology. 1998; 11(1); 27-36. [PubMed: 9586695].

3. Dijkstra et al., 1997: Dijkstra JM, Gerdts V, Klupp BG, Mettenleiter TC. Deletion of glycoprotein gM of pseudorabies virus results in attenuation for the natural host. The Journal of general virology. 1997; 78 ( Pt 9); 2147-2151. [PubMed: 9292000].

4. Eo et al., 2006: Eo SK, Yoon HA, Aleyas AG, Park SO, Han YW, Chae JS, Lee JH, Song HJ, Cho JG. Systemic and mucosal immunity induced by oral somatic transgene vaccination against glycoprotein B of pseudorabies virus using live attenuated Salmonella typhimurium. FEMS immunology and medical microbiology. 2006; 47(3); 451-461. [PubMed: 16872383].

5. Ferrari et al., 2000: Ferrari M, Brack A, Romanelli MG, Mettenleiter TC, Corradi A, Dal Mas N, Losio MN, Silini R, Pinoni C, Pratelli A. A study of the ability of a TK-negative and gI/gE-negative pseudorabies virus (PRV) mutant inoculated by different routes to protect pigs against PRV infection. Journal of veterinary medicine. B, Infectious diseases and veterinary public health. 2000; 47(10); 753-762. [PubMed: 11204130].

6. Fischer et al., 2003: Fischer T, Planz O, Stitz L, Rziha HJ. Novel recombinant parapoxvirus vectors induce protective humoral and cellular immunity against lethal herpesvirus challenge infection in mice. Journal of virology. 2003; 77(17); 9312-9323. [PubMed: 12915547].

7. Fuchs et al., 1990: Fuchs W, Rziha HJ, Lukàcs N, Braunschweiger I, Visser N, Lütticken D, Schreurs CS, Thiel HJ, Mettenleiter TC. Pseudorabies virus glycoprotein gI: in vitro and in vivo analysis of immunorelevant epitopes. The Journal of general virology. 1990; 71 ( Pt 5); 1141-1151. [PubMed: 1693164].

8. Grabowska et al., 2009: Grabowska AK, Lipińska AD, Rohde J, Szewczyk B, Bienkowska-Szewczyk K, Rziha HJ. New baculovirus recombinants expressing Pseudorabies virus (PRV) glycoproteins protect mice against lethal challenge infection. Vaccine. 2009; 27(27); 3584-3591. [PubMed: 19464538].

9. Han et al., 2008: Han YW, Aleyas AG, George JA, Kim SJ, Kim HK, Yoon HA, Yoo DJ, Kang SH, Kim K, Eo SK. Polarization of protective immunity induced by replication-incompetent adenovirus expressing glycoproteins of pseudorabies virus. Experimental & molecular medicine. 2008; 40(6); 583-595. [PubMed: 19116444].

10. Jöns et al., 1997: Jöns A, Gerdts V, Lange E, Kaden V, Mettenleiter TC. Attenuation of dUTPase-deficient pseudorabies virus for the natural host. Veterinary microbiology. 1997; 56(1-2); 47-54. [PubMed: 9228681].

11. Katayama et al., 1997: Katayama S, Okada N, Yoshiki K, Okabe T, Shimizu Y. Protective effect of glycoprotein gC-rich antigen against pseudorabies virus. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 1997; 59(8); 657-663. [PubMed: 9300361].

12. Kimman et al., 1994: Kimman TG, De Wind N, De Bruin T, de Visser Y, Voermans J. Inactivation of glycoprotein gE and thymidine kinase or the US3-encoded protein kinase synergistically decreases in vivo replication of pseudorabies virus and the induction of protective immunity. Virology. 1994; 205(2); 511-518. [PubMed: 7975253].

13. Matsuda et al., 1992: Matsuda Tsuchida A, Katayama S, Okada N, Okabe T, Sasaki N. Protection from pseudorabies virus challenge in mice by a combination of purified gII, gIII and gVI antigens. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 1992; 54(3); 447-452. [PubMed: 1322712].

14. Merck Vet Manual: Pseudorabies: Merck Veterinary Manual- Pseudorabies [http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/102200.htm]

15. Peeters et al., 1994: Peeters B, Bouma A, de Bruin T, Moormann R, Gielkens A, Kimman T. Non-transmissible pseudorabies virus gp50 mutants: a new generation of safe live vaccines. Vaccine. 1994; 12(4); 375-380. [PubMed: 8178562].

16. Shiau et al., 2001: Shiau AL, Chu CY, Su WC, Wu CL. Vaccination with the glycoprotein D gene of pseudorabies virus delivered by nonpathogenic Escherichia coli elicits protective immune responses. Vaccine. 2001; 19(23-24); 3277-3284. [PubMed: 11312026].

17. Vrublevskaia et al., 2007: Vrublevskaia VV, Musienko VS, Skarga IuIu, Morenkov OS. [Immunological characteristics of Aujeszky's disease virus glycoprotein]. Voprosy virusologii. 2007; 52(3); 33-37. [PubMed: 17601050].

18. Weigel et al., 2003: Weigel RM, Hahn EC, Scherba G. Survival and immunization of raccoons after exposure to pseudorabies (Aujeszky's disease) virus gene-deleted vaccines. Veterinary microbiology. 2003; 92(1-2); 19-24. [PubMed: 12488067].

19. Wiki: Pseudorabies: Pseudorabies [http://en.wikipedia.org/wiki/Pseudorabies]