VIOLIN: Vaccine Investigation and Online Information Network

Neisseria meningitidis

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. lpdA (Other)
2. ExbB (Protective antigen)
3. GNA1030 (Protective antigen)
4. GNA1162 (Protective antigen)
5. GNA1220 (Protective antigen)
6. GNA1870 (Protective antigen)
7. GNA1946 (Protective antigen)
8. GNA2001 (Protective antigen)
9. GNA2091 (Protective antigen)
10. GNA2132 (Protective antigen)
11. GNA33 (Protective antigen)
12. GNA992 (hsf) (Protective antigen)
13. gp120 (Protective antigen)
14. lctP (Protective antigen)
15. NadA (Protective antigen)
16. NspA from N. meningitidis MC58 (Protective antigen)
17. P1 (Protective antigen)
18. tbp2 (Protective antigen)
19. tbpA (Protective antigen)
20. metH (Virmugen)
21. rfaF (Virmugen)
22. siaD (Virmugen)
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

487

2. Disease:

Meningitis

3. Introduction

Neisseria meningitidis, also simply known as meningococcus, is a Gram-negative β-proteobacterium and member of the bacterial family Neisseriaceaeis. There are 13 serogroups based on different capsular polysaccharide structures. Among them, six serogroups (A, B, C, W-135, X, and Y) cause most life-threatening disease (Stephens et al., 2007).

4. Microbial Pathogenesis

Meningococcus is spread through the exchange of saliva and other respiratory secretions during activities like coughing, kissing, and chewing on toys. Those with impaired immunity may be easier to get meningococcus. Meningococcal virulence is related to both capsule expression, expression of other surface structures, and underlying genotype. N meningitidis pathogenesis is related to its ability to express and modify capsule with serogroups A, B, C, W-135, X, and Y. Capsules of N meningitidis facilitate transmission and colonisation, and protect the meningococcus from desiccation, phagocytic killing, opsonisation, and complement-mediated bactericidal killing. Outer membrane proteins (e.g., PorB, PorA , Opa and Opc) contribute to meningococcal virulence. Outer membrane porins are involved in host-cell interactions and as targets for bactericidal antibodies. Endotoxin, or lipo-oligosaccharide, a major component of the outer membrane, differs in structure from enteric endotoxins and is crucial in inflammatory signalling via Toll-like receptor 4 (TLR4) (Stephens et al., 2007).

5. Host Ranges and Animal Models

It only infects humans; there is no animal reservoir.

6. Host Protective Immunity

N. mengingitidis establishes systemic infections only in individuals who lack serum bacterial antibodies directed against the capsular or noncapsular (cell wall) antigens of the invading strain, or in patients deficient in the late-acting complement components. The presence of serum bactericidal IgG and IgM is probably the most important host factor in preventing invasive disease. The role of bactericidal antibodies in prevention of invasive disease explains why high attack rates are seen in infants from 6 to 9 months old, the time at which maternal antibodies are being lost. Individuals with complement deficiencies (C5, C6, C7, or C8) may develop meningococcemia despite protective antibody. This emphasizes the importance of the complement system in defense against meningococcal disease (Textbook of Bacteriology).

II. Vaccine Related Pathogen Genes

1. ExbB

Gene Name : ExbB
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0011186
NCBI Gene ID : 903370
NCBI Protein GI : 15677575
Locus Tag : NMB1729
Genbank Accession : AE002098
Protein Accession : NP_274732
Taxonomy ID : 122586
Gene Starting Position : 1812128
Gene Ending Position : 1812790
Gene Strand (Orientation) : -
Protein Name : biopolymer transport protein
Protein pI : 9.81
Protein Weight : 22358.4
Protein Length : 220
Protein Note : identified by similarity to EGAD:145333; match to protein family HMM PF01618

DNA Sequence : Show Sequence

>NC_003112.2:1812128-1812790 Neisseria meningitidis MC58 chromosome, complete genome
ATCAGCTATCCTTTTGATTAAGCAGGCGGACGTGCAAATCGTGCGCCATCGCATCCAAATCCTGGGTCAG
TATTTTTGTGCCGCGATTGAGGAAGTTGTATGCCAACACCGCCGGAATCGCCACGAACAAACCCGCCGCC
GTCGCCACCAGTGCCTCGCCAATCGGGCCGGCAACCGCCGCAATACTCATCTGCCCGCTTTGCCCGATAT
TGATCAGGGCGTGGTAAATCCCCCAAACCGTGCCGAACAGCCCGATAAACGGCGCGGTCGCGCCGATGGA
GGCAAGCGCGGTCATCCCGTAATCAAACCGGCGCATAATCTGCGCCATACTGTTGCGGATTTGAATGACC
AAATACTCGTTCAACGGCAAAGCCTGCGCCAGTTCGGACGCTTCGTTTCGGCGGTAGTTGCGGTAAGACT
GCAATGCCTCTTGCGCCAGTTTGGACAAAGGCGCATCGACGGCGCGCACTTTTTCGACCGCGTCGTTCAG
CGACAAAGTATCGCGCATATGCCGTTTGACGGCGGCATTCCCTTTGCGCGCCCGATACAGCTTGATGCAG
CGCAAGACAACCAAACACCACGTTACGATACTCATCAACAGCATCAACACAAACACACCAATCAGGACGG
GATCGCCCGATTCAAACACTAATTTCAAATTCA

Protein Sequence : Show Sequence

>NP_274732.1 biopolymer transport protein [Neisseria meningitidis MC58]
MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARKGNAAVKRHMRDTLSLNDAVEKVRAVD
APLSKLAQEALQSYRNYRRNEASELAQALPLNEYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLF
GTVWGIYHALINIGQSGQMSIAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDL
HVRLLNQKDS

Molecule Role : Protective antigen
Molecule Role Annotation : A total of 11 meningococcal proteins that are necessary for establishing systemic infection were expressed as recombinant antigens and assessed for their ability to protect mice against live bacterial challenge against N. meningitidis; ExbB, which is required for iron acquisition, elicited protective immunity (Sun et al., 2005).
Related Vaccine(s): N. meningitidis ExbB Protein Vaccine

2. GNA1030

Gene Name : GNA1030
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010983
NCBI Gene ID : 903167
NCBI Protein GI : 15676917
Locus Tag : NMB1030
Genbank Accession : AE002098
Protein Accession : NP_274064
Taxonomy ID : 122586
Gene Starting Position : 1045039
Gene Ending Position : 1045602
Gene Strand (Orientation) : -
Protein Name : hypothetical protein
Protein pI : 8.65
Protein Weight : 19439.53
Protein Length : 187
Protein Note : identified by similarity to EGAD:89838; match to protein family HMM PF04264

DNA Sequence : Show Sequence

>NC_003112.2:1045039-1045602 Neisseria meningitidis MC58 chromosome, complete genome
TTTATTGTTTGGCTGCCTCGATTTGGATGTCGATGCGGACGCTTTTGGTCATACCAACGTTAACGAGGTA
GTCCATGCCCCATTTGGTGCGGTCGATGGTGGTGCTGAAGTCGCCGCCACAAACTTCGGTTTTCTCCATC
GGGCTTTGGTAGCAGTTGAATTTTTCGGCTTTGAGTTTGACGGGGGCGGTTTTGCCGTGCATGGTCAGGT
TGCCGTCAACGGAAACCAGTTTTTTGCCGTTGAAGTTGAATTTGGTGGAAACAAAGCGGATGTCCGGATA
TTGGGCGGCATCGAAGATGTCGGCTGATTTCAGGTGGTCGGTAAAGTGTTGCGAACCGCTTTGCAGGTTG
GCAATGGGGATGGTGATGTCGATTTTACCGTCGCGTTTTGCTTGGTCGAACTCGACGGAACCGGTCAGAC
CGTAAAAACCGCCGACGTTGGTGCTGGTGTTGAAATGGTCGATGGCGAAACGGGCGTTGGCGTGATATTC
GTCCACTTTGTAGGTGGCGGCGGAGGCAGTACTGATGGCGGCGGCTGCGAGTGCGGCGAAGATGATTTTT
TTCA

Protein Sequence : Show Sequence

>NP_274064.1 hypothetical protein NMB1030 [Neisseria meningitidis MC58]
MKKIIFAALAAAAISTASAATYKVDEYHANARFAIDHFNTSTNVGGFYGLTGSVEFDQAKRDGKIDITIP
IANLQSGSQHFTDHLKSADIFDAAQYPDIRFVSTKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQ
SPMEKTEVCGGDFSTTIDRTKWGMDYLVNVGMTKSVRIDIQIEAAKQ

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen as shown in some assays (Giuliani et al., 2006)

3. GNA1162

Gene Name : GNA1162
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010976
NCBI Gene ID : 903582
NCBI Protein GI : 15677037
Locus Tag : NMB1162
Genbank Accession : AE002098
Protein Accession : NP_274189
Taxonomy ID : 122586
Gene Starting Position : 1167697
Gene Ending Position : 1168344
Gene Strand (Orientation) : -
Protein Name : hypothetical protein
Protein pI : 6.26
Protein Weight : 20946.7
Protein Length : 215

DNA Sequence : Show Sequence

>NC_003112.2:1167697-1168344 Neisseria meningitidis MC58 chromosome, complete genome
CTTATTTGGGCTGCTCTTCAACGAATCTCGGACCTTTCAAGATGCCGTTGTGAGAATAGGGCGACAGCAG
GTTGTATGCGGCGGTTTTGGAAACCTGATAACCGCGGTCGGTCAGGCTGTTGGCAATCTGATTGACCACT
GCGCTGACCAAAGCCCCCAACAGGCCGCTGTTGCTGTTGTTGCTGCCTTCGCGGATGCTGGCCGAACCCG
ACCACAACTCTTTTCCGTTGCGGGAATCGACCAGCCGTGCTTTGGCGGATACGGTCGTCACGCTGTCTAA
AATTTGATATGAAGTGCCGTATTCGGTAACCGTAATGTACAAAACCGCATCATTGCCGAAAATCTGATGC
AGTTTTTCCGGCCGGACGGCGTGAATATCGGCGGCATTGGTCAAGCCGTTTTGTTTGAAGGTTTCCTCCA
CGACTGCGGCGGGGAAGACGTAATAGCCGGCTTCGGAAAGCGGCGCGGCGGTCGAAGCCAGTACACCCCA
TGTTCCGTTGACATCGGGCGATTCGTTCAGCGGCGGAACCACCAAAATTGAAGCCGGTTTGCTTTCCTTG
AATGACGTGTAGTCGAAATCGGGCGCTTTTTGAACTTGGCAGGCAGACAGCGCCAACACGGCGGCAAGCC
CTAAAATCAAAGGTTTCA

Protein Sequence : Show Sequence

>NP_274189.1 hypothetical protein NMB1162 [Neisseria meningitidis MC58]
MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPLNESPDVNGTWGVLASTAAPLSEAGYY
VFPAAVVEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSR
NGKELWSGSASIREGSNNSNSGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFV
EEQPK

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen(Pizza et al., 2000)

4. GNA1220

Gene Name : GNA1220
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010975
NCBI Gene ID : 903642
NCBI Protein GI : 15677093
Locus Tag : NMB1220
Genbank Accession : AE002098
Protein Accession : NP_274245
Taxonomy ID : 122586
Gene Starting Position : 1224371
Gene Ending Position : 1225318
Gene Strand (Orientation) : -
Protein Name : hypothetical protein
Protein pI : 5.52
Protein Weight : 32124.16
Protein Length : 315
Protein Note : identified by similarity to GP:2984585; match to protein family HMM PF01145

DNA Sequence : Show Sequence

>NC_003112.2:1224371-1225318 Neisseria meningitidis MC58 chromosome, complete genome
CTTATTTGGCGGTTTTGCTGCTGTCGATAATTTTCATACCGGCAGAAATCAGGCTGCCGATGTCGGCAAC
ATTGGCGGGCATAATCAGCGTATTGCTTTCTTTGGCAAGATTGTTGAACGCAGCGACGTATTGTTCCGCA
ATCTTCAGATTGACCGCATCCGCACCGCCTTGGGTTTGAAGGGCGGCGGCAATTTGACGGATGGCTTCGG
CATTGGCTTCGGCAACAAGGCGCAAGGATTCCGCTTCACCTTTGGCGCGGTTGATGCGGGCGATTTTCTC
GGCATTTGACGCATTGACCGCAGCCTGAGCCTCGCCTTCGGATTGTTGGATTTCGGCTTCGCGCTGACCA
CTGGCAAGGTTGATTTGTTCGATTTTACGACCTTCGGATTCGGCGATACGGGCGCGTTTTTCGCGTTCGG
CAGTAATTTGCGCCTGCATTGAGCGAAGGATTTCTTGCGGCGGAACCAAGTCTTTAATCTCATAACGCAA
AACCTTCACACCCCAAGCCCCGGCCGCCTCGTCCAAAGCCGCAACAACAGTACTGTTGATTTCGTCGCGT
TCTTCAAACGTTTTGTCCAACTCCATACGCCCGATAACGGAACGCAGCGTCGTTTGGGCAAGCTGGGTAA
TCGCCATAATGTAGTTGCTCGAACCGTATGAGGCGAGTTTGGGGTCGGTTACTTGGAAATAGATGATGCC
GTCAACAGTCAGCTGCGTATTGTCGCGCGTGATGCAGACCTGGCTGGGTACGTCTAAAGGGATTTCTTTC
AGCGAATGGCGGTAGGCGACGCGGTCGATAAAGGGAATCAAAATATTCAAACCGGCCGTCAGGGCGCGAT
GGAAACGCCCCAGCCTTTCGACAACGTGGACTTCCTGTTGTGGGATGACAACAAAGGATTTGAAACCGAA
AACGGCGACGGCTACCAACAAGATAATGAAAAATTCCA

Protein Sequence : Show Sequence

>NP_274245.1 hypothetical protein NMB1220 [Neisseria meningitidis MC58]
MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGLNILIPFIDRVAYRHSLKEIPLDVPSQ
VCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVA
ALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQS
EGEAQAAVNASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNN
LAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen (Pizza et al., 2000)

5. GNA1870

Gene Name : GNA1870
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010981
NCBI Gene ID : 904318
NCBI Protein GI : 15677705
Locus Tag : NMB1870
Genbank Accession : AE002098
Protein Accession : NP_274866
3D structure: PDB ID : 1YS5
Taxonomy ID : 122586
Gene Starting Position : 1975217
Gene Ending Position : 1976179
Gene Strand (Orientation) : +
Protein Name : hypothetical protein
Protein pI : 9.11
Protein Weight : 32796.64
Protein Length : 320
Protein Note : also named factor H-binding protein (fHbp) or rLP-2086 (Pizza et al., 2008).

DNA Sequence : Show Sequence

>NC_003112.2:1975217-1976179 Neisseria meningitidis MC58 chromosome, complete genome
AATGCCGTCTGAACCGCCGTTCGGACGACATTTGATTTTTGCTTCTTTGACCTGCCTCATTGATGCGGTA
TGCAAAAAAAGATACCATAACCAAAATGTTTATATATTATCTATTCTGCGTATGACTAGGAGTAAACCTG
TGAATCGAACTGCCTTCTGCTGCCTTTCTCTGACCACTGCCCTGATTCTGACCGCCTGCAGCAGCGGAGG
GGGTGGTGTCGCCGCCGACATCGGTGCGGGGCTTGCCGATGCACTAACCGCACCGCTCGACCATAAAGAC
AAAGGTTTGCAGTCTTTGACGCTGGATCAGTCCGTCAGGAAAAACGAGAAACTGAAGCTGGCGGCACAAG
GTGCGGAAAAAACTTATGGAAACGGTGACAGCCTCAATACGGGCAAATTGAAGAACGACAAGGTCAGCCG
TTTCGACTTTATCCGCCAAATCGAAGTGGACGGGCAGCTCATTACCTTGGAGAGTGGAGAGTTCCAAGTA
TACAAACAAAGCCATTCCGCCTTAACCGCCTTTCAGACCGAGCAAATACAAGATTCGGAGCATTCCGGGA
AGATGGTTGCGAAACGCCAGTTCAGAATCGGCGACATAGCGGGCGAACATACATCTTTTGACAAGCTTCC
CGAAGGCGGCAGGGCGACATATCGCGGGACGGCGTTCGGTTCAGACGATGCCGGCGGAAAACTGACCTAC
ACCATAGATTTCGCCGCCAAGCAGGGAAACGGCAAAATCGAACATTTGAAATCGCCAGAACTCAATGTCG
ACCTGGCCGCCGCCGATATCAAGCCGGATGGAAAACGCCATGCCGTCATCAGCGGTTCCGTCCTTTACAA
CCAAGCCGAGAAAGGCAGTTACTCCCTCGGTATCTTTGGCGGAAAAGCCCAGGAAGTTGCCGGCAGCGCG
GAAGTGAAAACCGTAAACGGCATACGCCATATCGGCCTTGCCGCCAAGCAATA

Protein Sequence : Show Sequence

>NP_274866.1 hypothetical protein NMB1870 [Neisseria meningitidis MC58]
MPSEPPFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRSKPVNRTAFCCLSLTTALILTACSSGG
GGVAADIGAGLADALTAPLDHKDKGLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSR
FDFIRQIEVDGQLITLESGEFQVYKQSHSALTAFQTEQIQDSEHSGKMVAKRQFRIGDIAGEHTSFDKLP
EGGRATYRGTAFGSDDAGGKLTYTIDFAAKQGNGKIEHLKSPELNVDLAAADIKPDGKRHAVISGSVLYN
QAEKGSYSLGIFGGKAQEVAGSAEVKTVNGIRHIGLAAKQ

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen (Giuliani et al., 2006)

6. GNA1946

Gene Name : GNA1946
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010974
NCBI Gene ID : 904205
NCBI Protein GI : 15677776
Locus Tag : NMB1946
Genbank Accession : AE002098
Protein Accession : NP_274940
3D structure: PDB ID : 3GXA
Taxonomy ID : 122586
Gene Starting Position : 2041081
Gene Ending Position : 2041944
Gene Strand (Orientation) : -
Protein Name : outer membrane lipoprotein
Protein pI : 4.96
Protein Weight : 29236.71
Protein Length : 287
Protein Note : identified by similarity to EGAD:30554; match to protein family HMM PF03180

DNA Sequence : Show Sequence

>NC_003112.2:2041081-2041944 Neisseria meningitidis MC58 chromosome, complete genome
CTTATTTGGCTGCGCCTTCATTCCATGCGGCAGGGGATTTGTAGCCCTCGAAGCGTTTGTGCGCGTAGGC
TTTGAACGCGTCGGAGTTATAGGCCTCGGTTACGTCTTTAAGCCATTGGCTGTCTTTGTCGGCGGTTTTG
ACGGCAGACCAGTTGACATAGGCAAAGCTCGGTTCTTGGAACAGGGCTTCGGTCAGCTTCATGCCGCTGC
TTATGGCGTAGTTGCCGTTGACGACGGCAAAATCCACGTCGGCGCGGCTACGCGGCAGTTGCGCGGCTTC
AAGCTCGACGATTTTGATGTTTTTCAGGTTCTCGGCGATGTCCGCTTTGGATGCGGTCAACGGATTGATG
CCGTCTTTGAGTTTGATCCAACCCAGTTCGTCGAGCATCACCAAGACGCGGGCGAAGTTGGACGGGTCGT
TGGGCGCGGATACGGTGCTGCCGTCTTTGACTTCTTCCAGCGATTTCAGCTTGCCCGGGTACAGTCCCAA
AGGCGCGGTCGGCACTTGGAAGACTTCGGTGATGTCCAGATTGTGTTCTTTTTTGAAGTCGTCAAGATAG
GGTTTGTGTTGGAAGACGTTGATGTCCAACTCGCCCTCAGCCAATGCCAGATTCGGGCGTACATAGTCGG
TAAACTCGACCAGTTTGACGGTGTAGCCTTTTTTCTCCAGCTCGGCTTGGATTTGTTCTTTGACCATATC
GCCGAAGTCGCCGACGGTCGTGCCGAAGACGATTTCTTTTTTCGCCGCGCCGTTGTCGGCGGCGGCAGAA
GCGGATGCGGCGGGCGCGCTGTCTTTTTGACCGCCGCAGGCGGCGAGGATGAGCGCGAGTGCGGCGGCGG
AAAGGGTTTTGAAGAAGGTTTTCA

Protein Sequence : Show Sequence

>NP_274940.1 outer membrane lipoprotein [Neisseria meningitidis MC58]
MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKEIVFGTTVGDFGDMVKEQIQAELEKKG
YTVKLVEFTDYVRPNLALAEGELDINVFQHKPYLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVK
DGSTVSAPNDPSNFARVLVMLDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFA
VVNGNYAISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYKSPAA
WNEGAAK

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen (Pizza et al., 2000)

7. GNA2001

Gene Name : GNA2001
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010947
NCBI Gene ID : 904124
NCBI Protein GI : 15677829
Locus Tag : NMB2001
Genbank Accession : AE002098
Protein Accession : NP_274993
Taxonomy ID : 122586
Gene Starting Position : 2114380
Gene Ending Position : 2115135
Gene Strand (Orientation) : +
Protein Name : hypothetical protein
Protein pI : 10.78
Protein Weight : 26229.95
Protein Length : 251
Protein Note : identified by similarity to EGAD:91561; match to protein family HMM PF00877

DNA Sequence : Show Sequence

>NC_003112.2:2114380-2115135 Neisseria meningitidis MC58 chromosome, complete genome
TATGGATTCTTTTTTCAAACCGGCAGTTTGGGCGGTTTTGTGGCTGATGTTTGCCGTCCGCCCCGCCCTT
GCCGACGAGTTGACCAACCTGCTCAGCAGCCGCGAGCAGATTCTCAGACAGTTTGCCGAAGACGAACAGC
CCGTTTTACCCATCAACCGAGCCCCCGCCCGGCGGGCGGGCAATGCCGACGAACTCATCGGCAGCGCGAT
GGGGCTTAACGAACAGCCCGTTTTACCCGTCAACCGAGTCCCCGCCCGGCGGGCGGGCAATGCCGACGAA
CTCATCGGCAACGCGATGGGGCTTAACGAACAGCCCGTTTTACCCGTCAACCGAGCCCCCGCCCGGCGGG
CGGGCAATGCCGACGAACTCATCGGCAACGCGATGGGACTTTTGGGTATTGCCTACCGCTACGGCGGCAC
ATCGGTTTCTACCGGTTTTGACTGCAGCGGCTTCATGCAGCACATCTTCAAACGCGCCATGGGCATCAAC
CTGCCGCGCACGTCGGCAGAACAGGCACGGATGGGTACGCCGGTTGCCCGAAGCGAATTGCAGCCCGGAG
ATATGGTGTTTTTCCGCACGCTCGGCGGCAGCCGCATTTCCCATGTCGGACTTTATATCGGCAACAACCG
CTTCATCCACGCGCCGCGCACGGGGAAAAATATCGAAATCACCAGCCTGAGCCACAAATATTGGAGCGGC
AAATACGCGTTCGCCCGCCGGGTCAAGAAAAACGACCCGTCCCGCTTTCTGAACTG

Protein Sequence : Show Sequence

>NP_274993.1 hypothetical protein NMB2001 [Neisseria meningitidis MC58]
MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAGNADELIGSAM
GLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGT
SVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNR
FIHAPRTGKNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen (Pizza et al., 2000)

8. GNA2091

Gene Name : GNA2091
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010982
NCBI Gene ID : 903958
NCBI Protein GI : 15677911
Locus Tag : NMB2091
Genbank Accession : AE002098
Protein Accession : NP_275079
Taxonomy ID : 122586
Gene Starting Position : 2211862
Gene Ending Position : 2212470
Gene Strand (Orientation) : +
Protein Name : hemolysin
Protein pI : 10.22
Protein Weight : 19563.68
Protein Length : 202
Protein Note : identified by similarity to EGAD:28637; match to protein family HMM PF04972

DNA Sequence : Show Sequence

>NC_003112.2:2211862-2212470 Neisseria meningitidis MC58 chromosome, complete genome
GATGAAACCCAAACCGCACACCGTCCGCACCCTGATTGCCGCCATTTTCAGCCTTGCCCTTAGCGGCTGC
GTCAGCGCAGTAATCGGAAGCGCCGCCGTCGGCGCGAAATCCGCCGTCGACCGCCGAACCACCGGCGCGC
AAACCGACGACAACGTTATGGCGTTGCGTATCGAAACCACCGCCCGTTCCTATCTGCGCCAAAACAACCA
AACCAAAGGCTACACGCCCCAAATCTCCGTCGTCGGCTACAACCGCCACCTGCTGCTGCTCGGACAAGTC
GCCACCGAAGGCGAAAAACAGTTCGTCGGTCAGATTGCACGTTCCGAACAGGCCGCCGAAGGCGTGTACA
ACTATATTACCGTCGCCTCCCTGCCGCGCACTGCCGGCGACATCGCCGGCGACACTTGGAACACATCCAA
AGTCCGCGCCACGCTGTTGGGCATCAGCCCCGCCACACAGGCGCGCGTCAAAATCGTTACCTACGGCAAC
GTAACCTACGTTATGGGCATCCTCACCCCCGAAGAACAGGCGCAGATTACCCAAAAAGTCAGCACCACCG
TCGGCGTACAAAAAGTCATCACCCTCTACCAAAACTACGTCCAACGCTG

Protein Sequence : Show Sequence

>NP_275079.1 hemolysin [Neisseria meningitidis MC58]
MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTTGAQTDDNVMALRIETTARSYLRQNNQ
TKGYTPQISVVGYNRHLLLLGQVATEGEKQFVGQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSK
VRATLLGISPATQARVKIVTYGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen as shown in some assays (Giuliani et al., 2006)

9. GNA2132

Gene Name : GNA2132
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010946
NCBI Gene ID : 903357
NCBI Protein GI : 15677945
Locus Tag : NMB2132
Genbank Accession : AE002098
Protein Accession : NP_275117
Taxonomy ID : 122586
Gene Starting Position : 2238475
Gene Ending Position : 2239941
Gene Strand (Orientation) : -
Protein Name : transferrin-binding protein-like protein
Protein pI : 4.57
Protein Weight : 48171.55
Protein Length : 488
Protein Note : identified by similarity to EGAD:39989; match to protein family HMM PF01298

DNA Sequence : Show Sequence

>NC_003112.2:2238475-2239941 Neisseria meningitidis MC58 chromosome, complete genome
ATCAATCCTGCTCTTTTTTGCCGGCAAACACGCCGAATCCGCCCTTTTCCGCATCTGTCGGGCGATAGCT
GTATTTTCCCGCCACTTCCTCGCCGGCCGGGCCGTAAAACTTTCCGGAAACATCCCCGCTGCCATTTTCC
GTCCAAGTCCCCTTAAAGCCGTTTCCATCGATGGCGGCTTTGAATTTTTGCGTACCCATATGCAAATCAT
CGCCGCTGTCGATAATGCCGTCCACAGATTTGCTGCCGAAATCGACTTTTGCGGCAAACCTGCCCCTGGT
CGGGTACGGACGGCCGTTTTCCGTATGGAAATGCAGTACTTCGCCGTTGTACACGGCCGCGCCCGCAAGC
ATTTCGCCTTTTGCCGGTTCGCCTTGAACACGAAGGGCATACGATCCGCCGGGCAATTTTTCCGCCCCGT
AAGTCAGATACCGGTAATTCCCTTCGGGCGCGAAGATATTGCCGGAATGCCCCGTCAGGCTGACCGCTTC
CCCATCGACAATCAGCGTATCCGCCTGATTGACGGGAATCAGCGGCATCTCGGCCGGAAGCGACCGCCTC
GACCGTGCAGAACGCCTAAATCGCGCAAATGAAGTGGGTTTAGGTTTATAAAAGATAATATATTGATTGA
TTCCCTTCATCTGCACACTATCGGCAACCAAACCGACAAATTTATCATTCTTCCCATCTTTCTTGTAATT
ACTTATTTTGTCTGCATCACTTAATTTTTCAAATTCTGATTTTAGCTGTACTTCTTCATCCAAGAAATTA
TTGCCACTACAAGAATCGCCTTTACAGTGGGTCAACGTTATATTTTGCGACGGCCCGTCAATCAAAACGC
CATTAGCCAAATCAACCCTTCCAAAATTGCTACCGCCATTCGCAGGTGCAGGGTTTGACGCGGGGATGGG
ATCTGAAGAACCGGCGGCTTGATTGTTTCCGGCTTGATTTGCACCTTGGGCAGCCGTATTGCCGGCATTT
TGCCCGCCTGCCGACGGATCGTCCCCCTGCATTCCGTCCGCCGCATTTGCCATATCCGGTTGGTTTGCCG
GCTGAGACGATTCCCCGGCATCCGTTGCTTGATTTTCCATATTTCCGGCAAGCATATTCGGATCCGGGGT
GTGATTCGGTGTCGAACTATCTGTACCGGCGGCATTTTGCGGCATATCATTTTGTGCCACCTCGTCTTCA
TTTTTGGGATTATCCGCTGTTACCGCACCGCCATTGCCTGTATTTTCTTCCGAAACCGCCGCCATATCTT
GACTGCCTTGTGCGGATGGCGCGCCCTGTCCTTGAGAACCTGCCTGTGGCGCATCTTCCTTTGCCTCTGT
CTCTTTTTCAGAAACAACAGGGGCGGCAGGTTTTGACAGCGTGTCCGCCGACTTGACATCGGGCGATCCG
CCACCGCCGCCCCCGCAGGCTGAAAGGGCAAAAATACAAGCCATTGCGATTACGCTGCGTTTAAACA

Protein Sequence : Show Sequence

>NP_275117.1 transferrin-binding protein-like protein [Neisseria meningitidis MC58]
MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVSEKETEAKEDAPQAGSQGQGAPSAQGSQ
DMAAVSEENTGNGGAVTADNPKNEDEVAQNDMPQNAAGTDSSTPNHTPDPNMLAGNMENQATDAGESSQP
ANQPDMANAADGMQGDDPSAGGQNAGNTAAQGANQAGNNQAAGSSDPIPASNPAPANGGSNFGRVDLANG
VLIDGPSQNITLTHCKGDSCSGNNFLDEEVQLKSEFEKLSDADKISNYKKDGKNDKFVGLVADSVQMKGI
NQYIIFYKPKPTSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLTY
GAEKLPGGSYALRVQGEPAKGEMLAGAAVYNGEVLHFHTENGRPYPTRGRFAAKVDFGSKSVDGIIDSGD
DLHMGTQKFKAAIDGNGFKGTWTENGSGDVSGKFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen (Pizza et al., 2000).

10. GNA33

Gene Name : GNA33
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010978
NCBI Gene ID : 902136
NCBI Protein GI : 15675973
Locus Tag : NMB0033
Genbank Accession : AE002098
Protein Accession : NP_273099
Taxonomy ID : 122586
Gene Starting Position : 30910
Gene Ending Position : 32235
Gene Strand (Orientation) : -
Protein Name : membrane-bound lytic murein transglycosylase A
Protein pI : 10.02
Protein Weight : 45724.72
Protein Length : 441
Protein Note : identified by similarity to GP:974447; match to protein family HMM PF03562; match to protein family HMM PF06725

DNA Sequence : Show Sequence

>NC_003112.2:30910-32235 Neisseria meningitidis MC58 chromosome, complete genome
TTTACGGGCGGTATTCGGGCTTCATACCGTTGGGTAGGAGCTGCCAGACATATCCCGTGGTTTTCTGTTT
GCCGGCAAGTTCGCCGGCTTCGTCGCCGTATCCCCAAAAATAATCCACGCGCACCGCGCCTTTAATCGCG
CTGCCGGTATCCTGCGCCATAATCAGGCGGTTGAGGGCTTTGCGGGTAACCGGATGGGCGGTGGCGACAA
ATAAGGGCGCACCCAAGGTAATGTAGTGCCGGTCGACTGCGCCGGCATATTCCCCCATCAGCGGCGTGCC
CAGTGCGCCGACAGGGCCGTCATTGCTGCTTCCGGCAAGCTCGCGGAAAAAGATATAGCTGGGGTTTTGA
CCCAAAACTTCGGCGAGGCGTTGCGGATTTTGCCGCATATAAGACTTAATGCCCTGCATGGAGGTTTGTC
CGAGTTTGAGGTAGCCCTTATCCGCCATATAGCGTCCGATGGAAACGTAGGGATGTTCGTTTTTGTCGGC
ATAGCCGATGCGGATGTATTTGCCGGACGGGGTTTTCAGACGGCCCGAGCCTTGGATGTGCATAAAAAAA
AGTTCGACAGGGTCTTCGGCGTAACCGAGTATCGGGGCTTTGCCGTCAAGCGCGCCGCCGTTGATTTGGT
TGCGCGTGTGGTAGGGGAGGAAGCGGCTTCCTTCAAACCTGCCTTTGATTGCTGTTGTGCGCGCGGTGAT
GGGGAATCGGGAGAGGTCGGCGGTATGTGTGCCGCCGGTATTGTCGATTGTGCCGCTGTTTTTTCCCGTC
TGCCTGATGCGGACAAGGGCTTTTCCGCTCCGCAAACCGGCAGGCAGGGGGACGGAGATAAAATCGTCGG
GAATACCGTAAATCGGGAAGCGGGCTTGTGCCGTCCGCCTGTCGTCGCCCTTCAGCACCGGTTCGTAATA
GCCGGTAACCGTACCGGCAAGGCTTCCGTTGCCTGCAACCTGCCACGGCGTGAAATAGCGTTCAAAAAAC
TGTTTTGCCTGAAAGGAATGGACGGGGGTTTGAAAGGCTTGGGCGCACACATCCTGCCAGCCTTGGCGGT
TTTTCAAATTGGCGCAGCCGAGGCGGAAGGATTGCAGGCTTTTGGCGAAATCCTGCGCCGCCCAGTGGGG
CAGGGACAGGTGCGGTACAACGGTATAGACGGCCCCGCCGCCGCCGACCGTCGTTCCGGCGGGGTCGGGG
ATGCCGACCGGCCGGTCCGGGCCGTTGATGACGGATGTGTCGGGTTGCGGAAAGGTTTGGATGCTCTTGC
TTTGGCAGGCGGCGAGGATGGCGGCGGCGATGCCGTACAGGGCGGCGCGGAATAGGTATTTTTTCA

Protein Sequence : Show Sequence

>NP_273099.1 membrane-bound lytic murein transglycosylase A [Neisseria meningitidis MC58]
MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAVYTVVPHLSLP
HWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGY
YEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPI
TARTTAIKGRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYA
DKNEHPYVSIGRYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALG
TPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK
QKTTGYVWQLLPNGMKPEYRP

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen (Pizza et al., 2000)

11. GNA992 (hsf)

Gene Name : GNA992 (hsf)
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010977
NCBI Gene ID : 903112
NCBI Protein GI : 15676883
Locus Tag : NMB0992
Genbank Accession : AE002098
Protein Accession : NP_274028
Taxonomy ID : 122586
Gene Starting Position : 1005588
Gene Ending Position : 1007363
Gene Strand (Orientation) : -
Protein Name : adhesin
Protein pI : 5.98
Protein Weight : 56655.27
Protein Length : 591
Protein Note : identified by similarity to EGAD:131908; match to protein family HMM PF03895; match to protein family HMM PF05662
Protein Annotation : hsf or called GNA992

DNA Sequence : Show Sequence

>NC_003112.2:1005588-1007363 Neisseria meningitidis MC58 chromosome, complete genome
CTTACCACTGATAACCGACAGATGCGGAAGCACCGAAATGGCCGCGCGAATTGCCGGAAGCCGTGCCTTT
GATAATCCAATTTCCGCCGTCGGAAATACTGGAGTAGCCGATGGCGTAACCGGCTTCGCCGCGATAAGTG
CCGCCGCCGATCGCCATCATACTCTTGCCGGGCAAATACGCCTGAACCAGACCTGCGGTTGCAATCGCTT
GGGCGATGCCCGCACGCGCGTTGCCGTCCACATTGTCGATGCGGTTGTTCAAGTTTTGCGCCACGCCTTT
AAGTTGTGCGACGTTTGTAACATCCCCCTCTTTAACGCCCGGGGCGACATTGGTAATGCGGACGGGTTTG
TTGTCCTTCTTGCTGCCGACATTCAATGCGTCCCCATCCACGCTCAAAGTGGGCGCATCCGCCCCCGCGC
CGAGCGAAACGCTGGAAAACTGCGGGGTCATCGAAGTGGCGATGTCGATATTTTTACCGTTGCGGGTAAT
CTCGATGTTGTTGCCGGCATTAATGTTGACGGTTTCATCCATCTTTCCCTTGCTCGGCGAAACATTGCCG
CTGATGACTTTGCCCGAAGAACCTGCAACCGCTTTGGAATCCAAATTCCAACCGCTGTTTTGCAGCTGAT
TGACGTTTAGGGCATCGCCGACATTTACATCATACATAACAGTGATGTTGCCTTGATCATCTTTACTTAC
AGTCGCAGTTGTACCTTTACCACTAGCAAAGGTTACATTTGTGCCTGATGTAACGGTTTCAAACTTGTCA
GCTTGACCTGTTTGACCATTAGCGGTTGTTGTTTTCATTCTCCAACCAGCCTTGTTTACTGCATCAATCA
CTTCTTTTGCAGTCACTAAGCCTTCGCCTTCGTCTGTAGAAGAACCATTCTCGCCTTTGTCTTTACCAGT
AACCAACTTACCGTCTTTTTCTTTAATAACAGAAGTCTTCGCACCGATTTTAACTTCGGTTTTCTTGCCG
TTGTCTTTGCTTTCCACATTAACAGTCGTTGTTTTCGTATCTGCGCTCAAGAACTCGACTGTGTCGTAAG
TGCGGACGAAATCAACGTTATCGGAAGCTGTTGTACCGGGTTTAACGCCTTTAATGTTCCAGCCAGCGTT
TAATACGTCTTTAACGCTTGCCGCACGTTTTTTCTCGTCATCGGTAACGTTGTCGTTGGTTACGTTTGTG
GTCGCTCCGGTATTCAGCAGCGTATCGGTCAAAGTCGAACCAATACCGTTCAGATGAACCGTGGTGTCGC
CGTTCGTCCCAGCCGTTTCTTTCGCAAAATTCAAGCCTTTGGTGTCGCTTGTGATGTTGACTTTATTGCC
GTTTGCGCTAAACGATAATTTTTCAGTTCCAACACTGGTCAGATCTGTGAGGTCTTTTTTCAGCGAGTAG
GTGAAGTTTGTGCCGTTTTGTTTGATTTTCAGGTTGTCGCCGGCTTTGAGGGTGATTTCTCTGGCTGTTA
GTACTCCTTTCTCGTTGAAATATACTGCCCAATCTGAATTTTCTTCTACTTTTTCTTTTTCTCCCGTGCC
TTCTTTATCGGAATTGACTATCAACACGGCAACAGTGCGTTGTACGGGGTCTAAATATAAATCTTCTTCT
TGCTCTTCATTGTTAGCACTTGCCTGAACCGTTGCAAACAACAGTGTCGCCAATACGGCGGTCTTCACGG
TTGCGGAGGCGCGTTTGGTGTGGTTGCGTGTGAGCTCGGATACGACGACCCAGGCATTGAGGGCACTATT
CCAAATGATGCGGTATATTTTGTTCA

Protein Sequence : Show Sequence

>NP_274028.1 adhesin [Neisseria meningitidis MC58]
MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVKTAVLATLLFATVQASANNEEQEEDLYLDPVQRTVA
VLIVNSDKEGTGEKEKVEENSDWAVYFNEKGVLTAREITLKAGDNLKIKQNGTNFTYSLKKDLTDLTSVG
TEKLSFSANGNKVNITSDTKGLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKK
RAASVKDVLNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKTEVKIGAKTSV
IKEKDGKLVTGKDKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTGQADKFETVTSGTNVTF
ASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNLDSKAVAGSSGKVISGNVSPSKGKMDETV
NINAGNNIEITRNGKNIDIATSMTPQFSSVSLGAGADAPTLSVDGDALNVGSKKDNKPVRITNVAPGVKE
GDVTNVAQLKGVAQNLNNRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSS
ISDGGNWIIKGTASGNSRGHFGASASVGYQW

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen (Pizza et al., 2000)

12. gp120

Gene Name : gp120
Sequence Strain (Species/Organism) : Human immunodeficiency virus 1
NCBI Protein GI : 2326505
Other Database IDs : CDD:278917
GOA:O40515
InterPro: IPR000777
UniProtKB/TrEMBL: O40515
Taxonomy ID : 11676
Gene Strand (Orientation) : ?
Protein pI : 9.81
Protein Weight : 11668
Protein Length : 172
Protein Note : Envelope glycoprotein GP120; pfam00516

Protein Sequence : Show Sequence

>CAA74764.1 gp120, partial [Human immunodeficiency virus 1]
NGSLAEGEVVIRSENFTDNAKTIIVQLNESVVINCTRPNNNTRKNIHLGRGRSVYATEKIIGNVKQAHCN
ISRAKWNDTLKQIVEKLREQFGKNKTIVFNQSSGGDPEI

Molecule Role : Protective antigen
Molecule Role Annotation : he DNA construct induced a significant anti-polysaccharide antibody response that was bactericidal. Mice immunized with the DNA construct were subsequently protected against challenge with a lethal dose of N. meningitidis serogroup C.(Prinz et al., 2003)
Related Vaccine(s): N. meningitidis DNA vaccine P3A

13. lctP

Gene Name : lctP
Sequence Strain (Species/Organism) : Neisseria meningitidis
VO ID : VO_0011185
NCBI Protein GI : 254667969
Other Database IDs : CDD:224535
EnsemblGenomes-Gn:NMO_0425
EnsemblGenomes-Tr:CBA04244
GOA:C6S5G4
InterPro: IPR003804
UniProtKB/TrEMBL: C6S5G4
Taxonomy ID : 662598
Gene Strand (Orientation) : ?
Protein Name : L-lactate permease
Protein pI : 8.92
Protein Weight : 52305.14
Protein Length : 601
Protein Note : L-lactate permease [Energy production and conversion]; COG1620

Protein Sequence : Show Sequence

>CBA04244.1 L-lactate permease [Neisseria meningitidis alpha14]
MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYFGDAGMLLNATAASGLVKTLTPITVIF
GAIMFNRMMETTGCIDVIRKWLATISPNPVAQLMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLK
VAIFTLVMNSVPVSFGAVGTPTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRK
NLGFVAIAVFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQVAKA
LAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFGNIFGQDVSASYQTLYV
PAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLALMGALIMVQLMLVGGDNSMVKIIGKEFAA
MAGEHWVYFSPYLGAIGAFFSGSNTVSNLTFGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIA
VCTVLDVKNSEGAIIKKTVIPMAIYGVIAVVAAMIFFL

Molecule Role : Protective antigen
Molecule Role Annotation : A total of 11 meningococcal proteins that are necessary for establishing systemic infection were expressed as recombinant antigens and assessed for their ability to protect mice against live bacterial challenge against N. meningitidis; the lactate permease (LctP) elicited protective immunity (Sun et al., 2005).
Related Vaccine(s): N. meningitidis LctP Protein Vaccine

14. lpdA

Gene Name : lpdA
Sequence Strain (Species/Organism) : Neisseria meningitidis
VO ID : VO_0011181
NCBI Protein GI : 1017430
Other Database IDs : CDD:133458
CDD:304358
CDD:180579
CDD:278498
CDD:280934
GOA:Q51191
HSSP: 1OJT
InterPro: IPR000089
InterPro: IPR000815
InterPro: IPR001327
InterPro: IPR003016
InterPro: IPR004099
InterPro: IPR006258
InterPro: IPR011053
InterPro: IPR012999
InterPro: IPR013027
InterPro: IPR016156
UniProtKB/TrEMBL: Q51191
Taxonomy ID : 487
Gene Strand (Orientation) : ?
Protein Name : outer membrane protein P64k or PM-6
Protein pI : 4.94
Protein Weight : 56755.94
Protein Length : 685
Protein Note : Lipoyl domain of the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases. 2-oxo acid dehydrogenase multienzyme complexes, like pyruvate dehydrogenase (PDH), 2-oxoglutarate dehydrogenase (OGDH) and branched-chain 2-oxo acid...; cd06849

Protein Sequence : Show Sequence

>CAA57206.1 outer membrane protein P64k or PM-6, partial [Neisseria meningitidis]
MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKATMDIPAEVAGVVKEVKVKVGDKISEG
GLIVVVEAEGAATAPKAEAPATPAQEAPKAAAPAPQAAQFGGSADAEYDVVLGGGPGGYSAAFAAADEGL
KVAIVERYKTLGGVCLNVGCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKNGVVSRLTGG
LAGMAKRRKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPFIPEDP
RIIDSSGALALKEVPGKLLIIGAGIIGLEMGTVYSTLGSRLDVVEMMDGLMQGADRDLVKVWQKQNEYRF
DNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVAAGRAPNGKLISAEKAGVAVTDRGFIEVDK
QMRTNVPHIYAIVDIVGQPMLAHKAVHEGHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKA
SGRKITKANFPWAASGRAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIG
KTIHPHPTLGESIGMAAEVALGTCTDLPPQKKK

Molecule Role : Other
Molecule Role Annotation : Sera of the challenge group immunized with CCPS-P64kR showed a three-fold higher bactericidal response than the sera of the group immunized with the plain CCPS and they were able to protect against challenge with Neisseria meningococci in the infant rat protection model (Carmenate et al., 2004).
Related Vaccine(s): CCPS-P64kR

15. metH

Gene Name : metH
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
NCBI Gene ID : 903064
NCBI Protein GI : 15676837
Locus Tag : NMB0944
Genbank Accession : AE002098
Protein Accession : NP_273982
Taxonomy ID : 122586
Gene Starting Position : 957284
Gene Ending Position : 959560
Gene Strand (Orientation) : +
Protein Name : 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
Protein pI : 5.08
Protein Weight : 79718.7
Protein Length : 758
Protein Note : catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine to form methionine

DNA Sequence : Show Sequence

>gi|77358697:957284-959560 Neisseria meningitidis MC58, complete genome
CATGACAACATTACATTTCTCAGGCTTCCCGCGTGTCGGCGCCTTCCGCGAATTGAAATTCGCACAAGAA
AAATACTGGCGCAAAGAAATCAGCGAGCAAGAATTGCTGGCTGTTGCTAAAGACTTGCGCGAGAAAAACT
GGAAACACCAGGTCGCTGCCAACGCCGATTTCGTTGCCGTAGGCGATTTCACTTTCTACGACCACATCCT
CGACCTGCAAGTCGCCACCGGCGCGATTCCCGCCCGCTTCGGCTTCGACAGCCAAAACCTGTCTTTGGAA
CAATTCTTCCAACTGGCGCGCGGTAACAAAGACCAATTCGCTATCGAAATGACCAAATGGTTCGACACCA
ACTACCACTACTTGGTGCCTGAATTCCACGCCGATACCGAATTCAAAGCCAATGCCAAACACTATGTTCA
ACAACTGCAAGAAGCCCAAGCCCTCGGTCTGAAAGCCAAACCGACCGTTGTAGGTCCGTTGACTTTCCTG
TGGGTGGGTAAAGAAAAAGGCGCCGTCGAATTCGACCGTCTGAGCCTGTTGCCTAAACTGTTGCCTGTTT
ACGTTGAAATCCTGACTGCTTTGGTTGAAGCCGGTGCCGAGTGGATTCAAATCGACGAGCCTGCTTTGGC
TGTCGATTTGCCTAAAGAATGGGTGGAAGCCTACAAAGACGTTTACGCTACTTTGAGCAAAGTAAGTGCC
AAAATCCTGTTGAGCACTTACTTCGGTTCTGTTGCCGAACACGCCGCATTGTTGAAAGCCCTGCCTGTTG
ACGGTCTGCACATCGACTTGGTACGCGCCCCCGAGCAACTGGACGCGTTCGCCGACTACGACAAAGTCCT
GTCTGCCGGCGTGATTGACGGCCGCAACATTTGGCGCGCCAACCTGAACAAAGTTTTGGAAACTGTCGAG
CCTCTGCAAGCCAAACTGGGTGACCGTTTGTGGATTTCCAGCTCTTGCTCGCTGCTGCACACTCCATTTG
ACTTGTCAGTTGAAGAAAAACTGAAAGCCAACAAACCCGACCTGTACTCTTGGTTGGCATTCACCCTGCA
AAAAACCCAAGAATTGCGCGTTCTGAAAGCTGCATTGAACGAAGGCCGTGATTCTGTTGCCGAAGAACTC
GCCGCCAGCCAAGCTGCTGCCGACTCCCGTGCCAACAGCAGCGAAATCCATCGTGCAGACGTTGCCAAAC
GCCTGGCCGATTTGCCTGCCAACGCAGACCAACGCAAATCTCCATTTGCCGACCGTATCAAAGCGCAACA
AGCATGGTTGAACCTACCTCTGCTACCGACTACCAACATCGGTTCTTTCCCGCAAACCACCGAAATCCGC
CAGGCACGCTCAGCCTTCAAAAAAGGCGAACTGTCTGCCGCCGATTACGAAGCCGCGATGAAAAAAGAAA
TCGCCTTGGTGGTTGAAGAGCAAGAAAAACTGGACTTGGACGTACTGGTACACGGCGAAGCCGAGCGTAA
CGACATGGTTGAATACTTCGGCGAATTGTTGAGCGGTTTTGCATTCACTCAATACGGCTGGGTACAAAGC
TACGGCTCACGCTGCGTGAAACCACCGATTATCTTTGGCGACGTAAGCCGTCCTGAAGCCATGACCGTGG
CTTGGTCTACTTACGCACAAAGCCTGACCAAACGCCCGATGAAAGGTATGTTGACCGGCCCTGTAACCAT
TCTGCAATGGTCTTTCGTCCGCAACGACATTCCTCGCTCTACCGTGTGCAAACAAATCGCACTGGCTCTG
AACGACGAAGTATTGGATCTGGAAAAAGCCGGCATCAAAGTCATCCAAATTGACGAACCTGCCATCCGCG
AAGGCTTGCCGCTGAAACGCGCCGATTGGGATGCCTACCTGAACTGGGCGGGCGAATCCTTCCGCCTGTC
CTCTGCCGGTTGCGAAGACAGCACCCAAATCCACACTCATATGTGTTACTCCGAGTTCAACGATATCCTG
CCTGCGATTGCTGCAATGGATGCGGACGTGATCACCATCGAGACTTCACGTTCCGACATGGAACTCTTGA
CCGCGTTCGGCGAATTCCAATACCCGAACGACATCGGCCCGGGGGTTTACGACATCCACAGCCCGCGCGT
ACCGACAGAAGCCGAAGTGGAGCACCTGTTGCGCAAAGCCATCGAGGTTGTACCGGTTGAACGTCTGTGG
GTTAACCCGGACTGCGGCCTGAAAACACGCGGCTGGAAAGAAACTCTGGAACAACTCCAAGTAATGATGA
ACGTAACCCGAAAACTGCGTGCCGAATTGGCGAAATA

Protein Sequence : Show Sequence

>gi|15676837|ref|NP_273982.1| 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Neisseria meningitidis MC58]
MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLREKNWKHQVAANADFVAVGDFTFYDHIL
DLQVATGAIPARFGFDSQNLSLEQFFQLARGNKDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQ
QLQEAQALGLKAKPTVVGPLTFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALA
VDLPKEWVEAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADYDKVL
SAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKLKANKPDLYSWLAFTLQ
KTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRADVAKRLADLPANADQRKSPFADRIKAQQ
AWLNLPLLPTTNIGSFPQTTEIRQARSAFKKGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERN
DMVEYFGELLSGFAFTQYGWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTI
LQWSFVRNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAGESFRLS
SAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEFQYPNDIGPGVYDIHSPRV
PTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLEQLQVMMNVTRKLRAELAK

Molecule Role : Virmugen
Molecule Role Annotation : A metH mutant, in combination with a siaD mutation, is attenuated in mice and induces significant protection from challenge with wild type N. meningitidis (Li et al., 2004).
Related Vaccine(s): Neisseria meningitidis metH/siaD mutant vaccine

16. NadA

Gene Name : NadA
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010980
NCBI Gene ID : 904134
NCBI Protein GI : 15677822
Locus Tag : NMB1994
Genbank Accession : AE002098
Protein Accession : NP_274986
Taxonomy ID : 122586
Gene Starting Position : 2100382
Gene Ending Position : 2101476
Gene Strand (Orientation) : +
Protein Name : adhesin/invasin
Protein pI : 4.54
Protein Weight : 35292.78
Protein Length : 364
Protein Note : identified by similarity to GP:2735093; match to protein family HMM PF03895

DNA Sequence : Show Sequence

>NC_003112.2:2100382-2101476 Neisseria meningitidis MC58 chromosome, complete genome
AATGAGCATGAAACACTTTCCATCCAAAGTACTGACCACAGCCATCCTTGCCACTTTCTGTAGCGGCGCA
CTGGCAGCCACAAGCGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGTTGCTGCCTACAACAATG
GCCAAGAAATCAACGGTTTCAAAGCTGGAGAGACCATCTACGACATTGGTGAAGACGGCACAATTACCCA
AAAAGACGCAACTGCAGCCGATGTTGAAGCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACT
AACCTGACCAAAACCGTCAATGAAAACAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAA
TAGAAAAGTTAACAACCAAGTTAGCAGACACTGATGCCGCTTTAGCAGATACTGATGCCGCTCTGGATGA
AACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACATTTGCTGAAGAGACTAAGACAAATATC
GTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCATGCCGAAGCATTCAACGATA
TCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAATGAAGCCAAACA
GACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAAGCC
GAAGCTGCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACA
TCAAAGCTGATATCGCTACGAACAAAGCTGATATTGCTAAAAACTCAGCACGCATCGACAGCTTGGACAA
AAACGTAGCTAATCTGCGCAAAGAAACCCGCCAAGGCCTTGCAGAACAAGCCGCGCTCTCCGGCCTGTTC
CAACCTTACAACGTGGGTCGGTTCAATGTAACGGCTGCAGTCGGCGGCTACAAATCCGAATCGGCAGTCG
CCATCGGTACCGGCTTCCGCTTTACCGAAAACTTTGCCGCCAAAGCAGGCGTGGCAGTCGGCACTTCGTC
CGGTTCTTCCGCAGCCTACCATGTCGGCGTCAATTACGAGTGGTA

Protein Sequence : Show Sequence

>NP_274986.1 adhesin/invasin [Neisseria meningitidis MC58]
MSMKHFPSKVLTTAILATFCSGALAATSDDDVKKAATVAIVAAYNNGQEINGFKAGETIYDIGEDGTITQ
KDATAADVEADDFKGLGLKKVVTNLTKTVNENKQNVDAKVKAAESEIEKLTTKLADTDAALADTDAALDE
TTNALNKLGENITTFAEETKTNIVKIDEKLEAVADTVDKHAEAFNDIADSLDETNTKADEAVKTANEAKQ
TAEETKQNVDAKVKAAETAAGKAEAAAGTANTAADKAEAVAAKVTDIKADIATNKADIAKNSARIDSLDK
NVANLRKETRQGLAEQAALSGLFQPYNVGRFNVTAAVGGYKSESAVAIGTGFRFTENFAAKAGVAVGTSS
GSSAAYHVGVNYEW

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen (Giuliani et al., 2006; Comanducci et al., 2002). There is another nadA (quinolinate synthetase) [ Neisseria meningitidis MC58 ] with GeneID: 902509. However, according to sequence analysis, this nadA is not the same NadA that was found to be protective antigen (Giuliani et al., 2006; Comanducci et al., 2002).

17. NspA from N. meningitidis MC58

Gene Name : NspA from N. meningitidis MC58
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0010979
NCBI Gene ID : 902774
NCBI Protein GI : 15676561
Locus Tag : NMB0663
Genbank Accession : AE002098
Protein Accession : NP_273705
3D structure: PDB ID : 1P4T
Taxonomy ID : 122586
Gene Starting Position : 690297
Gene Ending Position : 690821
Gene Strand (Orientation) : -
Protein Name : outer membrane protein
Protein pI : 10.02
Protein Weight : 17106.9
Protein Length : 174
Protein Note : identified by similarity to EGAD:137514; match to protein family HMM PF02462

DNA Sequence : Show Sequence

>NC_003112.2:690297-690821 Neisseria meningitidis MC58 chromosome, complete genome
ATCAGAATTTGACGCGCACACCGGCGGACAGTTCGCCGGAACGGACGTTTTTGACAGTGTTGACTTTGCC
GATGTAGTTGTAGCGGTAGCCGGCATCCAAATCGACATTCGGGGTAACGGCATAGCTTACGCCCGTCAAT
ACGCCGAGGCCGATGGAGGTTTGGCTGAAGCTGTCGCTGCCGCCCAAGTCGACGGAGGCGCGGTTGAGGC
TCAAGCGCGCGCCGAGATACGGTTTGACGGGCGATTGGGTGTCGAAGTCGTAAATGGCGGACGCGCCGAT
GCTGTAAAGTTTGAAATCGGTGGATGGGGCTTTATAGTTTTTGTAGCGCGTGTAATCGACGGCGAAGCGG
AGGTCGTTGATGCGGTAGCCTGCGGAGATGCGCGGGCTGAAGCCTTTGGCAGAACCTAAAGAGCTTGAGG
CTTTTGCGTGTGCGGCATCGGCTTGGACGTAAAAGCCGGATGCGCCTTCCGCCAGTGCGGCGGCCGGGAG
AGCGAGGGCAATCAGTGTGGCAAGTGCTTTTTTCA

Protein Sequence : Show Sequence

>NP_273705.1 outer membrane protein [Neisseria meningitidis MC58]
MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAKGFSPRISAGYRINDLRFAVDYTRYKN
YKAPSTDFKLYSIGASAIYDFDTQSPVKPYLGARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPN
VDLDAGYRYNYIGKVNTVKNVRSGELSAGVRVKF

Molecule Role : Protective antigen
Molecule Role Annotation : Protective antigen (Martin et al., 1997)
NspA-containing outer membrane vesicles conferred protection against otherwise lethal intraperitoneal challenge of mice with N. meningitidis serogroup B, and sera raised against them mediated opsonophagocytosis of meningococcal strains expressing this antigen (O'dwyer et al., 2004).
Related Vaccine(s): N. meningitidis NspA Protein Vaccine

18. P1

Gene Name : P1
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
VO ID : VO_0011180
NCBI Gene ID : 902192
NCBI Protein GI : 15676020
Locus Tag : NMB0088
Genbank Accession : AE002098
Protein Accession : NP_273150
Taxonomy ID : 122586
Gene Starting Position : 99776
Gene Ending Position : 101176
Gene Strand (Orientation) : -
Protein Name : outer membrane protein P1
Protein pI : 9.85
Protein Weight : 47342.37
Protein Length : 466
Protein Note : identified by similarity to EGAD:16317; match to protein family HMM PF03349

DNA Sequence : Show Sequence

>NC_003112.2:99776-101176 Neisseria meningitidis MC58 chromosome, complete genome
TTTATTTGAATTTGTAGGTGTATTGCAGACCGATGATGTCGGCGTGGTTTTTGAAACGTGCGGAAGACGC
GCCTTTGCTGTCCACATCGTTGCCGCTTGCCTTCGCCGTGCGGTAGCTGGTGTCGTTGATGTGGATGTGG
GTGTAGGCGGCATCGACGACGTGGTTTTTACCGATATGGTATTTCATACCGGCGGAGAACCAGATGCGGT
TGCCGTCGGGTAGGCTGTTCATGCGGTAGTCGGCGTTGCGGACGGGCGATTTGTCAAAAGCGATGCCGGC
GCGCAGTTGCAGCGGTTCGCTGATTTGATAAGAACCGCCGAAGCCGACTTTGTAGGTGTTGCGCCAGTTG
GGGGTGATGGTGGTGCGGTCGGATTTGCCTTTGACGACGGTTTTTTCTTTTTCAAAAACCAGTTCCGCCT
TATCGAAGCGGCTGTGGCGCGTCCAAGTTACGTCGCCGAACAGGTCGGCTTTATCGGACACTTTGTACAT
ACCGTGTACGGACAAAGACTCAGGCGTAACGATTTTAACGCGGGCTTTTTCATTCGCCGTGTAGCCGTTT
GCTGCAAGCATCGTACTCCACATTGCTTTCGCCGCCGCGCCGTCTGCCGCCCATTCGGCATCGCCTTTGA
GCGTGTGCGAGACTTTGGAACGGTAGTTCACGCCCACGCGCGCACGGTCGTTGATGTCCCACATCCACGC
CAGTTGGTAGCCGAAGCCCCAATCGCTGCCTTTGACATCGGCGTGTCCGTCGGCCTGAATTTTTGCAGCT
TCGGCTACACCGTTAGGTTTGGGCGGTTTTGCCGTCAATATCTCTGCTTTACTCTTAATCCCCCAGTCGG
CATATTTGCGCAGTTCGGCGGAAGTATGTTGGGCGATGATGCCTGCGCCGAAGGAATGGCGGTCGTTGAG
TTTCCACGCGGCGACAGGTTCGACGGCGATGCTGGTCAGACCGAGTTTGTTGATGTTGTGGCGCAACACG
GAATCTTTTTCGTATTCGGTGGCAGAGCCGAAGGGGACGTACACGCCCAAGCCCACGGTCAGATTGTCGT
TGACTTTGTATGCGCCGTAGATGTGGGGCGCGACCGTGGTTTTGGTGATTTTGCCGCTTTTCGAACCTTG
GACGGGAAGCCCGGTAAAGTCGGTGGCGGAATCCGCCTCATAATGAATGCTGGGCAGCACGATGTTGGCG
TTGACGGAAATCTGGCTGCTGTCGAGTTTGGTCAGGCCGGCAGGGTTGTAGAAGATGGTCGATGCGTCGG
CGGCTTCTGCGGCGGCGGCATTTGCCGTGCTTTGCGCGTTGACCGACTGTGTGCCGAAGTGGTAGCCGGA
TGCGTGGACGGATGCGGCGGCAAAGGCAGTGCCGAGCAGCAGGACGGTTTTTTTCAGTGCGGAAGGGGTC
A

Protein Sequence : Show Sequence

>NP_273150.1 outer membrane protein P1 [Neisseria meningitidis MC58]
MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAAAEAADASTIFYNPAGLTKLDSSQISV
NANIVLPSIHYEADSATDFTGLPVQGSKSGKITKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKD
SVLRHNINKLGLTSIAVEPVAAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVA
EAAKIQADGHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWSTMLA
ANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKEKTVVKGKSDRTTIT
PNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSLPDGNRIWFSAGMKYHIGKNHVVDAAY
THIHINDTSYRTAKASGNDVDSKGASSARFKNHADIIGLQYTYKFK

Molecule Role : Protective antigen
Molecule Role Annotation : Seventeen monoclonal antibodies to one of three main cell surface antigens of Neisseria meningitidis group B were tested for protective efficacy in the infant rat using as challenge seven strains of different class 2/3 protein serotypes. Survival of the animals was regularly obtained with antibodies to the P1 protein and to LPS (Saukkonen et al., 1989).

19. rfaF

Gene Name : rfaF
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
NCBI Gene ID : 904029
NCBI Protein GI : 15677379
Locus Tag : NMB1527
Genbank Accession : AE002098
Protein Accession : NP_274534
Taxonomy ID : 122586
Gene Starting Position : 1577173
Gene Ending Position : 1578183
Gene Strand (Orientation) : -
Protein Name : ADP-heptose:LPS heptosyltransferase II
Protein pI : 7.02
Protein Weight : 35354.86
Protein Length : 336
Protein Note : identified by similarity to EGAD:40767; match to protein family HMM PF01075; match to protein family HMM TIGR02195

DNA Sequence : Show Sequence

>gi|77358697:1577173-1578183 Neisseria meningitidis MC58, complete genome
ATCATACCGCCTCTTCAACCGCCTGCACAATCTTCTCGGGATACAGCCTGTTGAGGCAGTCGGTATGCCC
CAGCGGACATTCCCGCTTAAAACACGGCGAACATTCCAAGTGCAGGCTGACGATTTTCGCCCTATCGCTC
AAAGGCGGCGTATGCGTCGGGCTGGAAGAACCGTAAACCGCCACCACCTTCCTGCCCAAAGCTGCCGCCA
AATGCATCAATCCGCTGTCGTTACACACGACCGTGTCCGCCAACGACAGCAAATCCATTGCCTGCGACAA
ATCGGTTTTGCCGCACAAATTGACACACATACCGTCTGAAAGGCGGTTGATTTCCTCGGCAATTTCATCA
TCTTTTTGCGAACCGAACAGCCAAACCTGCCAACCCGCCGCCAGATAATGTTTGCCCAACTCGGCAAAAT
GCCTTGTCGGCCAACGCTTTGCCGGCCCGAATTCCGCACCCGGACAAAAAGCCAGAACAGGCTTTCCAAT
ATCCAAGCCAAAGGTTTCGACAGAAATTTCCCGCCGCCGTTCATCAATGGAAAACTCGGGGAATCCCGAA
TGCCCGTCAAAATCTTCCTGACTCGGATGCGCGAGAGCCGTATATCGATCCACCATCAAAGGCAGACGTT
CCTTATCCAGCCTGCGTATATCGTTCAACAGAAAATAACGGCTTTCACCGACATAACCCGTCCTTTTACC
GATACCTGTCGCCAGCGCGATGATTGCCGATTTCAAAGAACCGGGCAACACGATAACCTGATCGTATCCG
CGCCGCCCCAAATCCCTACCGACCCGCCAACGGCGTTTCAACTCCAACGCACCATGTCCGAACGAATTCT
CAAGAATTTCATTCACTTCCGGCATACGCTCGAACACCGCCATCGACCACTTCGGTGCGAACACATCAAT
CGTGCAACCGGGGTGAAGTTCCTTCAAACGGCGGAACAAGGGCTGGGTCATCACGCAGTCGCCTATCCAA
CTGGGGGAAATAATCAGGATTTTGATGGACA

Protein Sequence : Show Sequence

>gi|15677379|ref|NP_274534.1| ADP-heptose:LPS heptosyltransferase II [Neisseria meningitidis MC58]
MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSMAVFERMPEVNEILENSFGHGALELKR
RWRVGRDLGRRGYDQVIVLPGSLKSAIIALATGIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTAL
AHPSQEDFDGHSGFPEFSIDERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAG
WQVWLFGSQKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKVVAVY
GSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEEAV

Molecule Role : Virmugen
Molecule Role Annotation : An rfaF mutant, in combination with a siaD mutation, is attenuated in mice and induces significant protection from challenge with wild type N. meningitidis (Li et al., 2004).
Related Vaccine(s): Neisseria meningitidis rfaF/siaD mutant vaccine

20. siaD

Gene Name : siaD
Sequence Strain (Species/Organism) : Neisseria meningitidis MC58
NCBI Gene ID : 903210
NCBI Protein GI : 15677983
Locus Tag : NMB0067
Genbank Accession : AE002098
Protein Accession : NP_273131
Taxonomy ID : 122586
Gene Starting Position : 76612
Gene Ending Position : 77289
Gene Strand (Orientation) : -
Protein Name : polysialic acid capsule biosynthesis protein SiaD
Protein pI : 10.48
Protein Weight : 25814.56
Protein Length : 225
Protein Note : identified by similarity to GP:530041

DNA Sequence : Show Sequence

>gi|77358697:76612-77289 Neisseria meningitidis MC58, complete genome
CTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGGCAAAAAACCGGTTATATTTTTTTGCATTAAATAT
TTTTTTAGCATATTCAGGAAAGGGGACATGCAATATGTCAAAATGATCTATATATCCTTTAATATTAAGA
TTATTTCCAATCAAATAACGTTCTAATTTTGTTGGATGATATGAAAATGATTCTAATAAAGGAGCATATG
TTCCAGTCCCTTCATCAATTAAATGAGTCGTAATATTCTTTTTTTTTGCAATACTAATCAGATAGGAGTA
GTGGCCTGTAAAAGACAGCATATAGAGATGAGCAGGCTGTATAATATTAAGGATTTTTTTGTAACTTCTA
TAAATATAAAGTAATTTTTTAGGAGTTATATTATTAGGGCTTCTAGGAAGCTCAAATAGATAAATAGATT
CAAATAGATTCTTGTTAGCTGATTGATGAACTAACTTAGGCATTTTTAAGTTTTTAGAAGTATATAAAAT
TACTAGTAAATTATTGGTTAATTTTTGTATTTTAATTAGGCTTTGGACTTGGTTAAGCTGACCTAAATTA
GATATGACAAATAAATTGTTACGTGGGGGGGTAAGATAAAATGGAGATGTTGTCAACCACATTGAATCTT
GAAAAAACTTTTTAGGCTGAAAAAGAGCTTTTTTTATTTTCTTTAGCA

Protein Sequence : Show Sequence

>gi|15677983|ref|NP_273131.1| polysialic acid capsule biosynthesis protein SiaD [Neisseria meningitidis MC58]
MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQLNQVQSLIKIQKLTNNLLVILYTSKN
LKMPKLVHQSANKNLFESIYLFELPRSPNNITPKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISI
AKKKNITTHLIDEGTGTYAPLLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKY
NRFFAWRKGDVLQGD

Molecule Role : Virmugen
Molecule Role Annotation : A siaD mutant, in combination with either a metH or rfaF mutation, is attenuated in mice and induces protection from challenge with wild type N. meningitidis (Li et al., 2004).
Related Vaccine(s): Neisseria meningitidis metH/siaD mutant vaccine , Neisseria meningitidis rfaF/siaD mutant vaccine

21. tbp2

Gene Name : tbp2
Sequence Strain (Species/Organism) : Neisseria meningitidis
VO ID : VO_0011182
NCBI Protein GI : NP_273507
Other Database IDs : CDD:307461
Taxonomy ID : 122586
Gene Strand (Orientation) : ?
Protein Name : transferrin-binding protein B
Protein pI : 5.85
Protein Weight : 74897.85
Protein Length : 797
Protein Note : Transferrin binding protein-like solute binding protein; pfam01298

Protein Sequence : Show Sequence

>NP_273507.1 transferrin-binding protein B [Neisseria meningitidis MC58]
MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKYQDVFSEKPQAQKDQGGYGFAMRLKRR
NWYPQAKEDEVKLDESDWEATGLPDEPKELPKRQKSVIEKVETDSDNNIYSSPYLKPSNHQNGNTGNGIN
QPKNQAKDYENFKYVYSGWFYKHAKREFNLKVEPKSAKNGDDGYIFYHGKEPSRQLPASGKITYKGVWHF
ATDTKKGQKFREIIQPSKSQGDRYSGFSGDDGEEYSNKNKSTLTDGQEGYGFTSNLEVDFHNKKLTGKLI
RNNANTDNNQATTTQYYSLEAQVTGNRFNGKATATDKPQQNSETKEHPFVSDSSSLSGGFFGPQGEELGF
RFLSDDQKVAVVGSAKTKDKPANGNTAAASGGTDAAASNGAAGTSSENGKLTTVLDAVELKLGDKEVQKL
DNFSNAAQLVVDGIMIPLLPEASESGNNQANQGTNGGTAFTRKFDHTPESDKKDAQAGTQTNGAQTASNT
AGDTNGKTKTYEVEVCCSNLNYLKYGMLTRKNSKSAMQAGESSSQADAKTEQVEQSMFLQGERTDEKEIP
SEQNIVYRGSWYGYIANDKSTSWSGNASNATSGNRAEFTVNFADKKITGTLTADNRQEATFTIDGNIKDN
GFEGTAKTAESGFDLDQSNTTRTPKAYITDAKVQGGFYGPKAEELGGWFAYPGDKQTKNATNASGNSSAT
VVFGAKRQQPVR

Molecule Role : Protective antigen
Molecule Role Annotation : Mice immunized with purified Tbp2 survived a lethal challenge to a similar degree as animals immunized with the Tbp1-Tbp2 complex, demonstrating that Tbp2 played an important role in the protective activity observed with the complex, eliciting antibodies that are not only bactericidal but also inhibitory for meningococcal growth (Lissolo et al., 1995).
Related Vaccine(s): N. miningitidis TBP2 Protein Vaccine

22. tbpA

Gene Name : tbpA
Sequence Strain (Species/Organism) : Neisseria meningitidis K454
VO ID : VO_0012380
NCBI Protein GI : 8926211
Other Database IDs : CDD:273799
CDD:238657
Taxonomy ID : 487
Gene Strand (Orientation) : ?
Protein Name : transferrin-binding protein A
Protein pI : 9.8
Protein Weight : 96910.19
Protein Length : 997
Protein Note : TbpA

Protein Sequence : Show Sequence

>AAF81744.1 transferrin-binding protein A [Neisseria meningitidis]
MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKAKKQKTRRDNEVTGLGKLVKSSDTLSK
EQVLNIRDLTRYDPGIAVVEQGRGASSGYSIRGMDKNRVSLTVDGVSQIQSYTAQAALGGTRTAGSSGAI
NEIEYENVKAVEISKGSNSVEQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALA
GRIGGAEALLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDSSNYAYFIVKEECKNGSYETCKANPKKDV
VGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHTQQTFDTRDMTVPAFLT
KAVFDANKKQAGSLPGNGKYAGNHKYGGLFTNGENGALVGAEYGTGVFYDETHTKSRYGLEYVYTNADKD
TWADYARLSYDRQGVGLDNHFQQTHCSADGSDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDT
AKIRHNLSVNLGFDRFGSNLRHQDYYYQHANRAYSSNTPPQNNGKKISPNGSETSPYWVTIGRGNVVTGQ
ICRLGNNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLSWNAGI
VLKPTDWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIVFKGDFGNLEASWFNNAY
RDLIVRGYEAQIKDGKEEAKGDPAYLNAQSARITGINILGKIDWNGVWDKLPEGWYSTFAYNRVRVRDIK
KRADRTDIQSHLFDAIQPSRYVVGLGYDQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATAR
RTRPWYIVDVSGYYTVKKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFS
LEMKF

Molecule Role : Protective antigen
Molecule Role Annotation : Recombinant TbpA protein was used in a mouse intraperitoneal-infection model to determine their ability to protect against meningococcal infection and to induce cross-reactive and bactericidal antibodies. TbpA was found to afford protection against meningococcal challenge when administered as the sole immunogen (West et al., 2001).
Related Vaccine(s): N. meningitidis TbpA Protein Vaccine

III. Vaccine Information

1. 5CVMB

a. Vaccine Ontology ID:

VO_0011348

b. Type:

Subunit vaccine

c. Status:

Licensed

d. Antigen

Five antigens are included: GNA2132, GNA1870, NadA, GNA1030, and GNA2091 (Giuliani et al., 2006)

e. Adjuvant:

VO ID: VO_0000127
Description: Adjuvant: aluminum hydroxide combined with CpG oligonucleotides (Giuliani et al., 2006)

f. Adjuvant:

VO ID: VO_0001237
Description: aluminum hydroxide combined with CpG oligonucleotides (Giuliani et al., 2006).

g. Immunization Route

Intraperitoneal injection (i.p.)

2. BEXSERO

a. Product Name:

Meningococcal Group B Vaccine

b. Tradename:

BEXSERO

c. Manufacturer:

Novartis Vaccines and Diagnostics, Inc

d. CDC CVX code:

163

e. Type:

Subunit vaccine

f. Status:

Licensed

g. Location Licensed:

USA, Canada

h. Host Species for Licensed Use:

Human

i. Allergen:

Latex

j. Immunization Route

Intramuscular injection (i.m.)

k. Storage

Store at 2°C to 8°C (36°F to 46°F), do not freeze, and keep away from light.

l . Approved Age for Licensed Use

10 to 25 years of age.

m. Description

BEXSERO (Meningococcal Group B Vaccine) is a sterile, white, opalescent, suspension for intramuscular injection. Each 0.5 mL dose of BEXSERO is formulated to contain 50 micrograms each of recombinant proteins Neisserial adhesin A (NadA), Neisserial Heparin Binding Antigen (NHBA), and factor H binding protein (fHbp), 25 micrograms of Outer Membrane Vesicles (OMV), 1.5 mg aluminum hydroxide (0.519 mg of Al3+), 3.125 mg sodium chloride, 0.776 mg histidine, and 10 mg sucrose at pH 6.4 – 6.7.(FDA: BEXSERO)

3. CCPS-P64kR

a. Vaccine Ontology ID:

VO_0004165

b. Type:

Conjugate vaccine

c. Status:

Research

d. Gene Engineering of lpdA

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

e. Adjuvant:

VO ID: VO_0000127

f. Immunization Route

Not specified

g. Rat Response

Vaccination Protocol: Rat pups, aged 5 or 6 days, were injected i.p. with 100 μl of sera ( from immunized mice) previously diluted 1:10 and ferric dextran (Carmenate et al., 2004).
Challenge Protocol: One hour after immunization, infant rats were challenged with 10^7 cfu of meningococcal strain Z4181. The development of bacteremia was assessed by culturing the blood samples taken 4 h after challenge (Carmenate et al., 2004).
Efficacy: Sera of the challenge group of mice immunized with CCPS-P64kR showed a three-fold higher bactericidal response than the sera of the group immunized with the plain CCPS and they were able to protect against challenge with Neisseria meningococci in the infant rat protection model (Carmenate et al., 2004).

h. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: Two experiments were carried out; in both cases mice were immunized with 2.5 μg of either plain or conjugated CCPS, and Al(OH)3 as adjuvant. Two doses were given 15 days apart and 7 days after the last dose mice were bled, sera were stored at −20°C. In the first experiment four groups of 10 mice each were immunized with the following immunogens: CCPS–P64kR, CCPS–P64kC, carrier protein P64k, and plain polysaccharide. For the second experiment, four groups of 10 mice each were immunized with the three conjugates obtained from different size polysaccharides using the reductive amination method and with plain CCPS as control (Carmenate et al., 2004).
Challenge Protocol: Sera of immunized mice was used in rats to detect an immune response (Carmenate et al., 2004).
Efficacy: Sera of the challenge group immunized with CCPS-P64kR showed a three-fold higher bactericidal response than the sera of the group immunized with the plain CCPS and they were able to protect against challenge with Neisseria meningococci in the infant rat protection model (Carmenate et al., 2004).

4. Menactra

a. Product Name:

Meningococcal Polysaccharide (Serogroups A, C, Y and W-135) Diphtheria Toxoid Conjugate Vaccine

b. Tradename:

Menactra

c. Manufacturer:

Sanofi Pasteur Inc.

d. Vaccine Ontology ID:

VO_0000071

e. CDC CVX code:

114

f. Type:

Conjugate vaccine

g. Status:

Licensed

h. Location Licensed:

USA (License #1725), Canada

i. Host Species for Licensed Use:

Human

j. Allergen:

Latex

k. Preparation

Cultured on Mueller Hinton agar and grown in Watson Scherp media. The polysaccharides are extracted from the N meningitidis cells and purified by centrifugation, detergent precipitation, alcohol precipitation, solvent extraction and diafiltration (FDA: Menactra).

l. Immunization Route

Intramuscular injection (i.m.)

m. Storage

Store at 2° to 8°C (35° to 46°F). DO NOT FREEZE.

n . Approved Age for Licensed Use

2-55 years of age.

o. Contraindication

This vaccine should not be administered to anyone with known hypersensitivity to any component of Menactra vaccine including diphtheria toxoid, or a life-threatening reaction after previous administration of a vaccine containing similar components (FDA: Menactra).

p. Description

Contains Neisseria meningitidis serogroup A, C, Y and W-135 capsular polysaccharide antigens individually conjugated to diphtheria toxoid protein. Menactra vaccine is not indicated for immunization against diphtheria (FDA: Menactra).

q. Human Response

Immune Response: Clinical studies reported antibodies were seen in 90-100% of those who were vaccinated with Menactra (FDA: Menactra).
Side Effects: Side effects of vaccination include: injection site reactions, headache, fatigue, irritability and fever.

5. MenHibrix

a. Product Name:

Meningococcal Groups C and Y and Haemophilus b Tetanus Toxoid Conjugate Vaccine

b. Tradename:

MenHibrix

c. Manufacturer:

GlaxoSmithKline Biologicals

d. Vaccine Ontology ID:

VO_0000031

e. Type:

Conjugate vaccine

f. Status:

Licensed

g. Location Licensed:

United States

h. Host Species for Licensed Use:

Human

i. Antigen

Neisseria meningitidis serogroup C and Y capsular polysaccharide antigens and
Haemophilus b capsular polysaccharide (polyribosyl-ribitol-phosphate [PRP]) (FDA: MenHibrix).

j. Immunization Route

Intramuscular injection (i.m.)

k . Approved Age for Licensed Use

6 months to 18 years (FDA: MenHibrix)

6. Meningitec

a. Product Name:

Meningococcal Group C Conjugate Vaccine (Diphtheria CRM197 Protein)

b. Tradename:

Meningitec

c. Manufacturer:

Berna Biotech, Wyeth Canada

d. Vaccine Ontology ID:

VO_0010724

e. Type:

Subunit vaccine

f. Status:

Licensed

g. Location Licensed:

Canada

h. Host Species for Licensed Use:

Human

i. Adjuvant:

VO ID: VO_0000193
Description: Products: Conjugate.

j. Allergen:

Latex in stopper of vial and plunger stopper of prefilled syringe

k. Immunization Route

Intramuscular injection (i.m.)

l. Storage

2°C to 8°C (36°F to 46°F).

m . Approved Age for Licensed Use

2 months of age and older.

n. Description

Products: Conjugate.

7. Meningococcal Polysaccharide Vaccine, Groups A & C, Menomune A/C

a. Tradename:

Meningococcal Polysaccharide Vaccine, Groups A & C, Menomune A/C

b. Manufacturer:

Sanofi Pasteur Ltd

c. Vaccine Ontology ID:

VO_0010725

d. Type:

Subunit vaccine

e. Status:

Licensed

f. Location Licensed:

Canada

g. Host Species for Licensed Use:

Human

h. Preservative:

Thimerosal

i. Allergen:

Latex in stopper of vial

j. Immunization Route

Subcutaneous injection

k. Storage

Store at at 2° to 8°C (35° to 46°F).

l . Approved Age for Licensed Use

2 years and older.

m. Description

Products: Polysaccharide. Other components: Lactose.

8. Menjugate

a. Product Name:

Meningococcal Group C CRM-197 Conjugate Vaccine

b. Tradename:

Menjugate

c. Manufacturer:

Novartis Vaccines and Diagnostics

d. Vaccine Ontology ID:

VO_0010726

e. Type:

Conjugate vaccine

f. Status:

Licensed

g. Location Licensed:

Canada

h. Host Species for Licensed Use:

Human

i. Adjuvant:

VO ID: VO_0000127

j. Immunization Route

Intramuscular injection (i.m.)

k. Storage

Do not store above 25° C.

l . Approved Age for Licensed Use

2 months and older.

m. Description

Products: Conjugate.

9. Menomune-A/C/Y/W-135

a. Product Name:

Meningococcal Polysaccharide Vaccine, Groups A, C, Y, W135 Combined

b. Tradename:

Menomune-A/C/Y/W-135

c. Manufacturer:

Sanofi Pasteur, Inc.

d. Vaccine Ontology ID:

VO_0000072

e. CDC CVX code:

f. Type:

Live, attenuated vaccine

g. Status:

Licensed

h. Location Licensed:

USA (License #1725)

i. Host Species for Licensed Use:

Human

j. Antigen

Meningococcal Polysaccharide Vaccine, Groups A, C, Y and W-135 Combined

k. Preparation

MEMOMUNE is cultivated with Mueller Hinton agar and Watson Scherp media and is freeze-dried with the group-specific polysaccharide antigens from Neisseria meningitidis, Group A, Group C, Group Y and Group W-135 (FDA: Menomune– A/C/Y/W-135).

l. Immunization Route

subcutaneous injection

m. Storage

Store the freeze-dried vaccine and reconstituted vaccine, when not in use, at 2° to 8°C (35° to 46°F).

n . Approved Age for Licensed Use

2 years of age and older

o. Contraindication

Individuals with known sensitivity to thimerosal or any other component of the vaccine.

p. Description

Meningococcal Polysaccharide Vaccine, Groups A, C, Y and W-135 Combined, is indicated for active immunization against invasive meningococcal disease caused by these serogroups (FDA: Menomune– A/C/Y/W-135).

q. Human Response

Immune Response: Measurable levels of antibodies against the group A and C polysaccharides decrease markedly during the first 3 years following a single dose of vaccine (FDA: Menomune– A/C/Y/W-135).
Side Effects: Side effects of vaccination include: pain and redness of injection site, fever and/or headache (FDA: Menomune– A/C/Y/W-135).
Efficacy: The serogroup A polysaccharide induces and antibody response in children as young as 3 months of age, the serogroup C component can be poorly immunogenic in recipients who are less than 18 to 24 months of age 5. The serogroups A and C have demonstrated estimated clinical efficacies of 85% to 100% in older children and adults. Serogroups Y and W-135 polysaccharides are safe and immunogenic in adults and in children greater than 2 years of age (FDA: Menomune– A/C/Y/W-135).

10. Menveo

a. Product Name:

Meningococcal (Groups A, C, Y and W-135) Oligosaccharide Diphtheria CRM197 Conjugate Vaccine

b. Tradename:

Menveo

c. Manufacturer:

Novartis Vaccines and Diagnostics, Inc.

d. Vaccine Ontology ID:

VO_0001246

e. CDC CVX code:

136

f. Type:

Conjugate vaccine

g. Status:

Licensed

h. Location Licensed:

USA, Canada

i. Host Species for Licensed Use:

Human

j. Antigen

N. meningitidis serogroup A, C, Y and W-135 oligosaccharides conjugated individually to Corynebacterium diphtheriae CRM197 protein (FDA: Menveo).

k. Preparation

The polysaccharides are produced by bacterial fermentation of N. meningitidis (serogroups A, C, Y or W-135). N. meningitidis strains A, C, Y and W-135 are each cultured and grown on Franz Complete medium and treated with formaldehyde. MenA, MenW-135 and MenY polysaccharides are purified by several extraction and precipitation steps. MenC polysaccharide is purified by a combination of chromatography and precipitation steps. The protein carrier (CRM197) is produced by bacterial fermentation and is purified by a series of chromatography and ultrafiltration steps. C. diphtheriae is cultured and grown on CY medium containing yeast extracts and amino acids. The oligosaccharides are prepared for conjugation from purified polysaccharides by hydrolysis, sizing, and reductive amination. After activation, each oligosaccharide is covalently linked to the CRM197 protein. The resulting glycoconjugates are purified to yield the four drug substances, which compose the final vaccine (FDA: Menveo).

l. Immunization Route

Intramuscular injection (i.m.)

m. Storage

Store refrigerated, away from the freezer compartment, at 36°F to 46°F (2°C to 8°C). Keep away from light (FDA: Menveo).

n . Approved Age for Licensed Use

MENVEO is approved for use in persons 11 to 55 years of age (FDA: Menveo).

o. Description

MENVEO [Meningococcal (Groups A, C, Y and W-135) Oligosaccharide Diphtheria CRM197 Conjugate Vaccine] is a sterile liquid vaccine administered by intramuscular injection that contains N. meningitidis serogroup A, C, Y and W-135 oligosaccharides conjugated individually to Corynebacterium diphtheriae CRM197 protein (FDA: Menveo).

11. N. meningitidis DNA vaccine P3A

a. Vaccine Ontology ID:

VO_0004569

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Antigen

a T-cell helper epitope and a peptide mimic of N. meningitidis serogroup C (Prinz et al., 2003)

f. Gene Engineering of gp120

Type: DNA vaccine construction
Description: The T-cell epitope used was from HIV gp120 (Prinz et al., 2003).
Detailed Gene Information: Click here.

g. Vector:

pcDNA3.1 (Prinz et al., 2003)

h. Immunization Route

Intramuscular injection (i.m.)

i. Mouse Response

Vaccine Immune Response Type: VO_0003057
Immune Response: Mice immunized with P3A DNA produced an anti-MCPS IgM antibody response that was significantly higher (P < 0.05) than that of mice immunized with P3C DNA (negative control) (Prinz et al., 2003).
Efficacy: Mice immunized with P3A DNA were protected against meningococcal infection, with 100% survival. Five of the six mice immunized with P3C DNA (negative control) died within 24 hr postchallenge (Prinz et al., 2003).

12. N. meningitidis ExbB Protein Vaccine

a. Vaccine Ontology ID:

VO_0004011

b. Type:

Subunit vaccine

c. Status:

Research

d. Gene Engineering of ExbB

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

e. Adjuvant:

VO ID: VO_0000142

f. Immunization Route

Subcutaneous injection

g. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: To examine the protective efficacy of recombinant proteins, adult mice (6-week-old, female BALB/c animals, 35 per group) were immunised on days 1 and 21 with 25 μg of antigen given by subcutaneous (s.c.) injection. Each protein was mixed with Freund's incomplete adjuvant (50% v/v) prior to administration. Mice in control groups were immunised with adjuvant alone or recombinant homologous PorA prior to challenge (Sun et al., 2005).
Challenge Protocol: 28 days after immunization, animals were challenged with live bacteria at either a high (10^7 CFU, 15 mice) or low (10^6 CFU, 15 mice) dose of MC58 (Sun et al., 2005).
Efficacy: ExbB, which is required for iron acquisition, elicited protective immunity and was able to protect mice from bacterial challenge (Sun et al., 2005).

13. N. meningitidis LctP Protein Vaccine

a. Vaccine Ontology ID:

VO_0004012

b. Type:

Subunit vaccine

c. Status:

Research

d. Gene Engineering of lctP

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

e. Adjuvant:

VO ID: VO_0000142

f. Immunization Route

Subcutaneous injection

g. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: To examine the protective efficacy of recombinant proteins, adult mice (6-week-old, female BALB/c animals, 35 per group) were immunised on days 1 and 21 with 25 μg of LctP given by subcutaneous (s.c.) injection. Each protein was mixed with Freund's incomplete adjuvant (50% v/v) prior to administration. Mice in control groups were immunised with adjuvant alone or recombinant homologous PorA prior to challenge (Sun et al., 2005).
Challenge Protocol: 28 days after immunization, animals were challenged with live bacteria at either a high (10^7 CFU, 15 mice) or low (10^6 CFU, 15 mice) dose of MC58 (Sun et al., 2005).
Efficacy: LctP which is required for iron acquisition, elicited protective immunity and was able to protect mice from bacterial challenge (Sun et al., 2005).

14. N. meningitidis NspA Protein Vaccine

a. Vaccine Ontology ID:

VO_0004013

b. Type:

Subunit vaccine

c. Status:

Research

d. Gene Engineering of NspA from N. meningitidis MC58

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

e. Adjuvant:

VO ID: VO_0001263
Description: QuilA (CedarLane Laboratories, Hornby, Ontario, Canada) (Martin et al., 1997).

f. Immunization Route

Intraperitoneal injection (i.p.)

g. Mouse Response

Vaccination Protocol: Groups of mice were injected three times at 3-wk intervals with 10 or 20 μg of affinity-purified NspA recombinant protein and 25 μg of QuilA (CedarLane Laboratories, Hornby, Ontario, Canada) as the adjuvant. Control mice were injected with either 20 μg of BSA (Sigma), concentrated E. coli BL21(DE3) supernatant, or PBS. 2 wk after the third injection the mice were used for the protection experiments (Martin et al., 1997).
Challenge Protocol: For inoculation of mice, meningococci were removed from the chocolate agar plates after ∼20 h of incubation and suspended in PBS and injected into mice (Martin et al., 1997).
Efficacy: 80% of the mice immunized with three injections of either 10 or 20 μg of purified recombinant meningococcal NspA protein survived the bacterial challenge comparatively to 0 to 20% in the control groups. Survivors at 72 h did not succumb during an additional two weeks of observation. The mice in the control group injected with concentrated E. coli culture supernatant were not protected against the bacterial challenge indicating that the components present in the culture media and other E. coli antigens that might be present in small amounts after purification do not contribute to the observed protection against N. meningitidis (Martin et al., 1997).

15. N. meningitidis TbpA Protein Vaccine

a. Vaccine Ontology ID:

VO_0004064

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

rTbpA

e. Gene Engineering of tbpA

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0000139
Description: Vaccines were prepared with an equal volume of either Freund's complete adjuvant (first immunization) or Freund's incomplete adjuvant (subsequent immunizations) (West et al., 2001).

g. Adjuvant:

VO ID: VO_0000142
Description: Vaccines were prepared with an equal volume of either Freund's complete adjuvant (first immunization) or Freund's incomplete adjuvant (subsequent immunizations) (West et al., 2001).

h. Immunization Route

subcutaneous injection

i. Mouse Response

Host Strain: NIH
Vaccination Protocol: Each mouse received 0.2 ml, containing 10 μg of rTbpA protein and Freund's complete adjuvant, by subcutaneous injection. All animals were immunized on days 1, 21, and 28 (West et al., 2001).
Challenge Protocol: Mice were infected by intraperitoneal injection of N. meningitidis at several challenge doses (West et al., 2001).
Efficacy: 100% protection was afforded by vaccination with rTbpA at the 2 × 10^7 CFU challenge dose andat the 2 × 10^8 CFU challenge dose, the rTbpA-vaccinated group had an 85% survival rate (West et al., 2001).

16. N. miningitidis TBP2 Protein Vaccine

a. Vaccine Ontology ID:

VO_0004014

b. Type:

Subunit vaccine

c. Status:

Research

d. Gene Engineering of tbp2

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

e. Adjuvant:

VO ID: VO_0000127

f. Immunization Route

Subcutaneous Injection

g. Mouse Response

Vaccination Protocol: Groups of 48 mice were immunized subcutaneously on days 0, 21, and 35 with different vaccine preparations containing 5 mg of protein adsorbed onto 0.1 mg of aluminum hydroxide per 0.5 ml; PBS containing aluminum hydroxide and heat-inactivated N. meningitidis B16B6 were used as controls (Lissolo et al., 1995).
Challenge Protocol: 41 days after immunization, mice within a group were subdivided into groups of eight, and each subgroup received by the intraperitoneal route 24 mg of iron-loaded hTf (Sigma) and, immediately after by the intraperitoneal route, 0.5 ml of N. meningitidis grown under iron restriction. The bacterial load varied from 10^4 to 10^8 CFU. Mortality rates were measured for 5 days following challenge (Lissolo et al., 1995).
Efficacy: Mice immunized with purified Tbp2 survived a lethal challenge to a similar degree as animals immunized with the Tbp1-Tbp2 complex, demonstrating that Tbp2 played an important role in the protective activity observed with the complex, eliciting antibodies that are not only bactericidal but also inhibitory for meningococcal growth (Lissolo et al., 1995).

17. Neisseria meningitidis metH/siaD mutant vaccine

a. Type:

Live, attenuated vaccine

b. Status:

Research

c. Host Species as Laboratory Animal Model:

Mouse

d. Gene Engineering of metH

Type: Gene mutation
Description: This metH/siaD mutant is from Neisseria meningitidis (Li et al., 2004).
Detailed Gene Information: Click here.

e. Gene Engineering of siaD

Type: Gene mutation
Description: This metH/siaD mutant is from Neisseria meningitidis (Li et al., 2004).
Detailed Gene Information: Click here.

f. Gene Engineering of siaD

Type: Gene mutation
Description: This metH/siaD mutant is from Neisseria meningitidis (Li et al., 2004).
Detailed Gene Information: Click here.

g. Immunization Route

Intraperitoneal injection (i.p.)

h. Mouse Response

Persistence: A metH/siaD mutant is attenuated in mice (Li et al., 2004).
Efficacy: A metH/siaD mutant induces significant protection in mice from challenge with wild type neisseria meningitidis (Li et al., 2004).

18. Neisseria meningitidis rfaF/siaD mutant vaccine

a. Type:

Live, attenuated vaccine

b. Status:

Research

c. Host Species as Laboratory Animal Model:

Mouse

d. Gene Engineering of rfaF

Type: Gene mutation
Description: This rfaF/siaD mutant is from Neisseria meningitidis (Li et al., 2004).
Detailed Gene Information: Click here.

e. Gene Engineering of siaD

Type: Gene mutation
Description: This rfaF/siaD mutant is from Neisseria meningitidis (Li et al., 2004).
Detailed Gene Information: Click here.

f. Immunization Route

Intraperitoneal injection (i.p.)

g. Mouse Response

Persistence: A rfaF/siaD mutant is attenuated in mice (Li et al., 2004).
Efficacy: An rfaF/siaD mutant induces significant protection in mice from challenge with wild type Neisseria meningitidis (Li et al., 2004).

19. Neisvac-C

a. Product Name:

Meningococcal Group C-TT Conjugate Vaccine, Adsorbed

b. Tradename:

Neisvac-C

c. Manufacturer:

Baxter Healthcare Corporation, GlaxoSmithKline

d. Vaccine Ontology ID:

VO_0010729

e. Type:

Conjugate vaccine

f. Status:

Licensed

g. Location Licensed:

Canada

h. Host Species for Licensed Use:

Human

i. Adjuvant:

VO ID: VO_0000127

j. Immunization Route

Intramuscular injection (i.m.)

k. Storage

Store at 2°C to 8°C.

l . Approved Age for Licensed Use

2 months of age and older.

m. Description

Products: Conjugate.

20. NIMENRIX

a. Product Name:

Meningococcal polysaccharide groups A, C, W-135 and Y conjugate vaccine

b. Tradename:

NIMENRIX

c. Manufacturer:

Pfizer Inc.

d. Type:

Conjugate vaccine

e. Status:

Licensed

f. Location Licensed:

Canada

g. Host Species for Licensed Use:

Human

h. Immunization Route

Intramuscular injection (i.m.)

i. Storage

Store at 2°C to 8°C (36°F to 46°F), do not freeze, and protect from light.

j . Approved Age for Licensed Use

1 to 55 years of age.

k. Description

NIMENRIX® (meningococcal polysaccharide groups A, C, W-135 and Y conjugate vaccine) is a tetravalent meningococcal polysaccharide conjugated vaccine consisting of Neisseria
meningitidis capsular polysaccharides A, C, W-135 and Y each coupled to tetanus toxoid as a carrier protein. The Neisseria meningitidis serogroups A and C polysaccharides are conjugated with an adipic dihydrazide (AH) spacer and indirectly conjugated to the tetanus toxoid whereas the W-135 and Y polysaccharides are conjugated directly to tetanus toxoid. (Pfizer: NIMENRIX)

21. TRUMENBA

a. Product Name:

Meningococcal Group B Vaccine

b. Tradename:

TRUMENBA

c. Manufacturer:

Pfizer Inc.

d. Type:

Subunit vaccine

e. Status:

Licensed

f. Location Licensed:

USA

g. Host Species for Licensed Use:

Human

h. Immunization Route

Intramuscular injection (i.m.)

i. Storage

Store at 2°C to 8°C (36°F to 46°F), do not freeze.

j . Approved Age for Licensed Use

10 to 25 years of age.

k. Description

Trumenba is a sterile suspension composed of two recombinant lipidated factor H binding protein (fHBP) variants from N. meningitidis serogroup B, one from fHBP subfamily A and one from subfamily B (A05 and B01, respectively). The proteins are individually produced in E. coli. Production strains are grown in defined fermentation growth media to a specific density. The recombinant proteins are extracted from the production strains and purified through a series of column chromatography steps. Polysorbate 80 (PS80) is added to the drug substances and is present in the final drug product. (FDA: TRUMENBA)

IV. References

1. Carmenate et al., 2004: Carmenate T, Canaán L, Alvarez A, Delgado M, González S, Menéndez T, Rodés L, Guillén G. Effect of conjugation methodology on the immunogenicity and protective efficacy of meningococcal group C polysaccharide-P64k protein conjugates. FEMS immunology and medical microbiology. 2004; 40(3); 193-199. [PubMed: 15039094].

2. Comanducci et al., 2002: Comanducci M, Bambini S, Brunelli B, Adu-Bobie J, Aricò B, Capecchi B, Giuliani MM, Masignani V, Santini L, Savino S, Granoff DM, Caugant DA, Pizza M, Rappuoli R, Mora M. NadA, a novel vaccine candidate of Neisseria meningitidis. The Journal of experimental medicine. 2002; 195(11); 1445-1454. [PubMed: 12045242].

3. FDA: BEXSERO: FDA: BEXSERO vaccine information [https://www.fda.gov/downloads/BiologicsBloodVaccines/Vaccines/ApprovedProducts/UCM431447.pdf]

4. FDA: Menactra: FDA: Menactra [https://www.fda.gov/downloads/BiologicsBloodVaccines/Vaccines/ApprovedProducts/UCM131170.pdf]

5. FDA: MenHibrix: FDA: MenHibrix Package insert [http://www.fda.gov/BiologicsBloodVaccines/Vaccines/ApprovedProducts/ucm308566.htm]

6. FDA: Menomune– A/C/Y/W-135: FDA Menomune– A/C/Y/W-135 Neisseria meningitidis vaccine [https://www.fda.gov/downloads/BiologicsBloodVaccines/Vaccines/ApprovedProducts/UCM308370.pdf]

7. FDA: Menveo: FDA: Menveo [http://www.fda.gov/BiologicsBloodVaccines/Vaccines/ApprovedProducts/ucm201342.htm]

8. FDA: TRUMENBA: FDA: TRUMENBA vaccine information [https://www.fda.gov/downloads/BiologicsBloodVaccines/Vaccines/ApprovedProducts/UCM421139.pdf]

9. Giuliani et al., 2006: Giuliani MM, Adu-Bobie J, Comanducci M, Aricò B, Savino S, Santini L, Brunelli B, Bambini S, Biolchi A, Capecchi B, Cartocci E, Ciucchi L, Di Marcello F, Ferlicca F, Galli B, Luzzi E, Masignani V, Serruto D, Veggi D, Contorni M, Morandi M, Bartalesi A, Cinotti V, Mannucci D, Titta F, Ovidi E, Welsch JA, Granoff D, Rappuoli R, Pizza M. A universal vaccine for serogroup B meningococcus. Proceedings of the National Academy of Sciences of the United States of America. 2006; 103(29); 10834-10839. [PubMed: 16825336].

10. GSK: Menjugate: GSK: Menjugate vaccine information [https://ca.gsk.com/media/1213633/menjugate.pdf]

11. Li et al., 2004: Li Y, Sun YH, Ison C, Levine MM, Tang CM. Vaccination with attenuated Neisseria meningitidis strains protects against challenge with live Meningococci. Infection and immunity. 2004; 72(1); 345-351. [PubMed: 14688114].

12. Li et al., 2014: Li S, Rouphael N, Duraisingham S, Romero-Steiner S, Presnell S, Davis C, Schmidt DS, Johnson SE, Milton A, Rajam G, Kasturi S, Carlone GM, Quinn C, Chaussabel D, Palucka AK, Mulligan MJ, Ahmed R, Stephens DS, Nakaya HI, Pulendran B. Molecular signatures of antibody responses derived from a systems biology study of five human vaccines. Nature immunology. 2014; 15(2); 195-204. [PubMed: 24336226].

13. Lissolo et al., 1995: Lissolo L, Maitre-Wilmotte G, Dumas P, Mignon M, Danve B, Quentin-Millet MJ. Evaluation of transferrin-binding protein 2 within the transferrin-binding protein complex as a potential antigen for future meningococcal vaccines. Infection and immunity. 1995; 63(3); 884-890. [PubMed: 7868259].

14. Martin et al., 1997: Martin D, Cadieux N, Hamel J, Brodeur BR. Highly conserved Neisseria meningitidis surface protein confers protection against experimental infection. The Journal of experimental medicine. 1997; 185(7); 1173-1183. [PubMed: 9104804].

15. O'dwyer et al., 2004: O'dwyer CA, Reddin K, Martin D, Taylor SC, Gorringe AR, Hudson MJ, Brodeur BR, Langford PR, Kroll JS. Expression of heterologous antigens in commensal Neisseria spp.: preservation of conformational epitopes with vaccine potential. Infection and immunity. 2004; 72(11); 6511-6518. [PubMed: 15501782].

16. Pfizer: NeisVac-C: Pfizer: NeisVac-C vaccine information [http://www.pfizer.ca/sites/g/files/g10017036/f/201505/NeisVac-C_PM_182023_31Mar2015_EN.pdf]

17. Pfizer: NIMENRIX: Pfizer: NIMENRIX vaccine information [http://www.pfizer.ca/sites/g/files/g10028126/f/201604/Nimenrix_PM_184020_23Mar2016_E.pdf]

18. Pizza et al., 2000: Pizza M, Scarlato V, Masignani V, Giuliani MM, Aricò B, Comanducci M, Jennings GT, Baldi L, Bartolini E, Capecchi B, Galeotti CL, Luzzi E, Manetti R, Marchetti E, Mora M, Nuti S, Ratti G, Santini L, Savino S, Scarselli M, Storni E, Zuo P, Broeker M, Hundt E, Knapp B, Blair E, Mason T, Tettelin H, Hood DW, Jeffries AC, Saunders NJ, Granoff DM, Venter JC, Moxon ER, Grandi G, Rappuoli R. Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing. Science (New York, N.Y.). 2000; 287(5459); 1816-1820. [PubMed: 10710308].

19. Pizza et al., 2008: Pizza M, Donnelly J, Rappuoli R. Factor H-binding protein, a unique meningococcal vaccine antigen. Vaccine. 2008; 26 Suppl 8; I46-48. [PubMed: 19388164].

20. Prinz et al., 2003: Prinz DM, Smithson SL, Kieber-Emmons T, Westerink MA. Induction of a protective capsular polysaccharide antibody response to a multiepitope DNA vaccine encoding a peptide mimic of meningococcal serogroup C capsular polysaccharide. Immunology. 2003; 110(2); 242-249. [PubMed: 14511238].

21. Saukkonen et al., 1989: Saukkonen K, Leinonen M, Abdillahi H, Poolman JT. Comparative evaluation of potential components for group B meningococcal vaccine by passive protection in the infant rat and in vitro bactericidal assay. Vaccine. 1989; 7(4); 325-328. [PubMed: 2510417].

22. Stephens et al., 2007: Stephens DS, Greenwood B, Brandtzaeg P. Epidemic meningitis, meningococcaemia, and Neisseria meningitidis. Lancet. 2007; 369(9580); 2196-2210. [PubMed: 17604802].

23. Sun et al., 2005: Sun Y, Li Y, Exley RM, Winterbotham M, Ison C, Smith H, Tang CM. Identification of novel antigens that protect against systemic meningococcal infection. Vaccine. 2005; 23(32); 4136-4141. [PubMed: 15964482].

24. Textbook of Bacteriology: Staphylococcus bacteriaPrint this Page Pathogenic Neisseriae: Gonorrhea, Neonatal Ophthalmia and Meningococcal Meningitis [http://textbookofbacteriology.net/neisseria]

25. West et al., 2001: West D, Reddin K, Matheson M, Heath R, Funnell S, Hudson M, Robinson A, Gorringe A. Recombinant Neisseria meningitidis transferrin binding protein A protects against experimental meningococcal infection. Infection and immunity. 2001; 69(3); 1561-1567. [PubMed: 11179327].