VIOLIN: Vaccine Investigation and Online Information Network

rinderpest virus

Table of Contents

General Information
1. NCBI Taxonomy ID
Vaccine Related Pathogen Genes
1. H protein (Protective antigen)
2. RPVgp5 F (Protective antigen)
3. RPVgp6 H (Protective antigen)
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

11241

II. Vaccine Related Pathogen Genes

1. H protein

Gene Name : H protein
Sequence Strain (Species/Organism) : rinderpest virus
NCBI Protein GI : YP_087125
Other Database IDs : CDD:271231
GOA:Q629I1
InterPro: IPR000665
InterPro: IPR011040
UniProtKB/TrEMBL: Q629I1
Taxonomy ID : 11242
Protein Name : H protein
Protein pI : 7.44
Protein Weight : 64611.9
Protein Length : 679
Protein Note : Measles virus hemagglutinin; cd15467

Protein Sequence : Show Sequence

>YP_087125.2 H protein [Rinderpest virus (strain Kabete O)]
MSPPRDRVDAYYKDNFQFKNTRVVLNKEQLLIERPCMLLTVLFVMFLSLVGLLAIAGIRLHRAAVNTAKI
NNDLTTSIDITKSIEYQVKDVLTPLFKIIGDEVGLRTPQRFTDLTKFISDKIKFLNPDKEYDFRDINWCI
NPPERIKIDYDQYCAHTAAEDLITMLVNSSLTGTTVLRTSLVNLGRNCTGPTTTKGQFSNISLTLSGIYS
GRGYNISSMITITGKGMYGSTYLVGKYNQRARRPSIVWQQDYRVFEVGIIRELGVGTPVFHMTNYLELPR
QPELETCMLALGESKLAALCLADSPVALHYGRVGDDNKIRFVKLGVWASPADRDTLATLSAIDPTLDGLY
ITTHRGIIAAGTAIWAVPVTRTDDQVKMGKCRLEACRDRPPPFCNSTDWEPLEAGRIPAYGVLTIKLGLA
DEPKVDIISEFGPLITHDSGMDLYTSFDGTKYWLTTPPLQNSALGTVNTLVLEPSLKISPNILTLPIRSG
GGDCYTPTYLSDRADDDVKLSSNLVILPSRDLQYVSATYDISRVEHAIVYHIYSTGRLSSYYYPFKLPIK
GDPVSLQIECFPWDRKLWCHHFCSVIDSGTGEQVTHIGVVGIEITCNGK

Molecule Role : Protective antigen
Molecule Role Annotation : (Khandelwal et al., 2004)
Related Vaccine(s): RPV ISCOM Vaccine

2. RPVgp5 F

Gene Name : RPVgp5 F
Sequence Strain (Species/Organism) : Rinderpest virus (strain Kabete O)
NCBI Gene ID : 3021776
NCBI Protein GI : 56410434
Locus Tag : RPVgp5
Genbank Accession : X98291
Protein Accession : YP_087124
Taxonomy ID : 11242
Gene Starting Position : 5457
Gene Ending Position : 7097
Gene Strand (Orientation) : +
Protein Name : F protein
Protein pI : 8.22
Protein Weight : 55061.12
Protein Length : 546

DNA Sequence : Show Sequence

>NC_006296.2:5457-7097 Rinderpest virus (strain Kabete O), complete genome
CATGAAGATCTTATTTGCTACCCTGCTAGTAGTGACCACCCCTCACTTAGTCACTGGCCAGATCCACTGG
GGCAATCTTTCGAAAATTGGAGTCGTAGGGACGGGTAGTGCTAGTTACAAGGTGATGACCCAATCGAGTC
ACCAGACACTAGTTATAAAGCTGATGCCCAATATCACCGCTATAGACAACTGCACTAAGACTGAGATTGA
GGAGTATAAGAGGCTGTTGGGGACAGTGCTTCAGCCTATTAAGGTCGCTCTGAACGCCATTACTAAGAAT
ATAAAACCAATCAGGAGTTCCACTACCAGTAGGAGACACAGAAGGTTCGCAGGGGTCGCTTTGGCTGGTG
CAGCTCTCGGAGTTGCAACCGCAGCCCAGATTACAGCAGGGATTGCTCTTCATCAATCAATGATGAATAC
CCAGGCTATTGAAAGTCTTAAGGCAAGTCTGGAAACAACTAATCAAGCAATTGAGGAAATACGACAAGCA
GGCCAAGAAATGATCCTAGCAGTTCAAGGTGTCCAGGACTACATCAACAATGAACTGGTACCTGCAATGG
GCCAATTATCGTGTGATATCGTGGGTCAGAAGCTAGGGCTGAAACTGCTCAGATACTACACTGAAATATT
ATCATTATTTGGCCCCAGCCTCAGAGACCCAATTTCGGCCGAGATTTCTATCCAGGCTTTGAGTTACGCC
TTAGGTGGGGACATAAACAAAATACTAGAAAAGCTAGGGTATAGCGGGAGTGATCTCCTTGCTATACTGG
AAAGTAAAGGTATCAAAGCTAAGATAACCTATGTGGATATCGAAAGTTACTTCATTGTGCTCAGCATTGC
CTATCCTTCGCTTTCTGAAATTAAAGGGGTGATAATTCATCGCCTGGAGGGTGTCTCTTACAATATAGGG
TCGCAGGAGTGGTATACCACAGTACCTCGATATGTGGCAACCCAAGGTTACCTCATTTCGAATTTCGATG
ACACACCCTGTGCATTCTCCCCAGAGGGCACTATCTGCAGCCAGAATGCATTATACCCAATGAGCCCACT
CCTCCAGGAATGTTTCCGTGGGTCAACCAGGTCATGCGCTCGTACACTGGTCTCTGGGTCTATAGGGAAT
AGGTTTATCCTATCCAAGGGGAACCTCATTGCCAACTGTGCCTCAATCTTGTGCAAGTGTTACACCACTG
GCTCAATAATAAGTCAAGATCCTGACAAGATCCTAACATATATAGCTGCAGACCAATGCCCCATCGTAGA
AGTGGACGGTGTAACCATCCAGGTTGGGAGCAGAGAGTACCCTGATGCAGTGTACCTGCATAAAATAGAC
CTTGGCCCACCAATATCGCTGGAGAAGCTAGATGTTGGGACTAACCTCGGCAATGCAGTGACAAAACTGG
AGAAAGCCAAGGATCTACTAGATTCATCTGATCTGATCCTGGAAACCATCAAGGGTGCTTCAGTCACGAA
CACAGGCCATATTCTAGTTGGAGCCGGGTTGATTGCAGTAGTGGGTATCCTCATTGTTACCTGTTGCTGT
AGGAAGCGCAGTAATGACAGCAAAGTGTCCACTGTGATATTGAATCCGGGTCTTAAACCAGACCTCACTG
GTACATCCAAATCCTACGTACGGTCACTGTA

Protein Sequence : Show Sequence

>YP_087124.2 F protein [Rinderpest virus (strain Kabete O)]
MKILFATLLVVTTPHLVTGQIHWGNLSKIGVVGTGSASYKVMTQSSHQTLVIKLMPNITAIDNCTKTEIE
EYKRLLGTVLQPIKVALNAITKNIKPIRSSTTSRRHRRFAGVALAGAALGVATAAQITAGIALHQSMMNT
QAIESLKASLETTNQAIEEIRQAGQEMILAVQGVQDYINNELVPAMGQLSCDIVGQKLGLKLLRYYTEIL
SLFGPSLRDPISAEISIQALSYALGGDINKILEKLGYSGSDLLAILESKGIKAKITYVDIESYFIVLSIA
YPSLSEIKGVIIHRLEGVSYNIGSQEWYTTVPRYVATQGYLISNFDDTPCAFSPEGTICSQNALYPMSPL
LQECFRGSTRSCARTLVSGSIGNRFILSKGNLIANCASILCKCYTTGSIISQDPDKILTYIAADQCPIVE
VDGVTIQVGSREYPDAVYLHKIDLGPPISLEKLDVGTNLGNAVTKLEKAKDLLDSSDLILETIKGASVTN
TGHILVGAGLIAVVGILIVTCCCRKRSNDSKVSTVILNPGLKPDLTGTSKSYVRSL

Molecule Role : Protective antigen
Related Vaccine(s): v2RVFH

3. RPVgp6 H

Gene Name : RPVgp6 H
Sequence Strain (Species/Organism) : Rinderpest virus (strain Kabete O)
NCBI Gene ID : 3021774
NCBI Protein GI : 56410435
Locus Tag : RPVgp6
Genbank Accession : X98291
Protein Accession : YP_087125
Taxonomy ID : 11242
Gene Starting Position : 7258
Gene Ending Position : 9087
Gene Strand (Orientation) : +
Protein Name : H protein
Protein pI : 7.04
Protein Weight : 63930.13
Protein Length : 609

DNA Sequence : Show Sequence

>NC_006296.2:7258-9087 Rinderpest virus (strain Kabete O), complete genome
CATGTCTCCCCCAAGAGACAGGGTCGACGCTTACTACAAGGACAACTTCCAATTCAAGAACACTCGGGTG
GTTCTTAATAAAGAGCAGCTCCTGATAGAGAGACCTTGCATGTTGCTGACGGTGTTGTTTGTCATGTTCC
TGAGTCTAGTCGGGCTATTAGCTATCGCAGGTATCAGACTCCATCGAGCTGCTGTCAATACAGCAAAAAT
CAACAATGACCTGACAACAAGTATTGATATTACCAAATCCATTGAGTACCAGGTCAAGGATGTTTTAACT
CCACTCTTCAAAATAATTGGAGATGAGGTCGGGTTGAGGACGCCTCAGAGATTCACAGATCTGACTAAAT
TCATATCAGACAAGATCAAGTTCCTTAACCCTGATAAGGAGTACGACTTTAGAGATATCAACTGGTGCAT
CAACCCTCCAGAGAGAATTAAGATTGATTATGATCAGTATTGTGCTCACACAGCAGCTGAGGATCTGATA
ACAATGCTTGTCAATTCATCCTTAACAGGGACTACAGTACTCCGCACGTCATTAGTCAACTTGGGGAGGA
ACTGTACTGGACCCACAACCACTAAGGGTCAGTTCTCTAATATATCATTGACCCTTTCCGGGATATACTC
AGGTCGTGGCTACAACATTTCATCTATGATCACTATTACTGGGAAAGGCATGTACGGGAGCACTTATCTG
GTCGGGAAATACAATCAAAGAGCCAGGAGGCCAAGCATAGTATGGCAACAGGATTACCGAGTCTTCGAAG
TAGGCATAATTAGAGAACTAGGAGTGGGCACACCAGTGTTTCATATGACAAACTACCTGGAACTTCCAAG
ACAGCCGGAATTGGAGACTTGCATGCTAGCCCTAGGAGAGTCCAAATTAGCTGCCCTCTGCCTAGCTGAC
AGCCCCGTCGCACTGCACTACGGGAGGGTAGGAGACGACAACAAGATCAGATTTGTCAAATTGGGAGTAT
GGGCATCACCGGCCGACCGAGACACTTTGGCCACTCTTTCAGCAATAGACCCGACATTGGACGGACTCTA
TATCACAACTCATAGGGGGATCATAGCTGCAGGGACTGCCATATGGGCTGTCCCTGTGACGAGAACAGAT
GACCAAGTGAAAATGGGAAAGTGCCGCCTAGAGGCTTGTCGAGACAGACCACCACCTTTCTGTAACAGTA
CAGATTGGGAGCCACTAGAGGCTGGCCGAATACCGGCGTATGGAGTACTAACCATCAAACTAGGGCTGGC
TGATGAGCCTAAAGTTGACATAATTTCAGAATTTGGTCCTCTAATCACCCATGACTCAGGGATGGATTTA
TACACCTCATTTGACGGTACCAAGTACTGGTTGACTACTCCTCCATTGCAGAACTCAGCTCTAGGAACTG
TGAACACCCTAGTTTTAGAGCCCAGTCTCAAAATTAGTCCTAACATCCTTACTCTCCCCATCAGGTCGGG
AGGGGGTGACTGTTATACTCCTACTTACCTGTCAGACCGGGCTGACGATGATGTTAAATTGAGCTCCAAT
CTTGTAATCCTCCCAAGTAGGGACCTCCAATATGTGTCTGCAACCTACGACATCTCCAGAGTTGAACATG
CCATTGTGTACCACATCTATAGTACCGGGCGACTATCATCGTATTACTACCCTTTCAAGTTGCCCATAAA
GGGAGATCCTGTCAGTCTGCAGATCGAATGCTTCCCGTGGGACCGCAAACTGTGGTGCCATCATTTCTGC
TCTGTTATAGATTCAGGGACTGGTGAGCAGGTCACCCATATAGGGGTAGTAGGGATTGAGATCACTTGCA
ATGGGAAATA

Protein Sequence : Show Sequence

>YP_087125.2 H protein [Rinderpest virus (strain Kabete O)]
MSPPRDRVDAYYKDNFQFKNTRVVLNKEQLLIERPCMLLTVLFVMFLSLVGLLAIAGIRLHRAAVNTAKI
NNDLTTSIDITKSIEYQVKDVLTPLFKIIGDEVGLRTPQRFTDLTKFISDKIKFLNPDKEYDFRDINWCI
NPPERIKIDYDQYCAHTAAEDLITMLVNSSLTGTTVLRTSLVNLGRNCTGPTTTKGQFSNISLTLSGIYS
GRGYNISSMITITGKGMYGSTYLVGKYNQRARRPSIVWQQDYRVFEVGIIRELGVGTPVFHMTNYLELPR
QPELETCMLALGESKLAALCLADSPVALHYGRVGDDNKIRFVKLGVWASPADRDTLATLSAIDPTLDGLY
ITTHRGIIAAGTAIWAVPVTRTDDQVKMGKCRLEACRDRPPPFCNSTDWEPLEAGRIPAYGVLTIKLGLA
DEPKVDIISEFGPLITHDSGMDLYTSFDGTKYWLTTPPLQNSALGTVNTLVLEPSLKISPNILTLPIRSG
GGDCYTPTYLSDRADDDVKLSSNLVILPSRDLQYVSATYDISRVEHAIVYHIYSTGRLSSYYYPFKLPIK
GDPVSLQIECFPWDRKLWCHHFCSVIDSGTGEQVTHIGVVGIEITCNGK

Molecule Role : Protective antigen
Related Vaccine(s): v2RVFH

III. Vaccine Information

1. rFP-RPV-H/F

a. Vaccine Ontology ID:

VO_0004752

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Preparation

Rinderpest virus (RPV) antigens, expressed in pox virus vectors (Jones et al., 1997).

f. Immunization Route

Intramuscular injection (i.m.)

g. Ferret Response

Vaccination Protocol: Ferrets were inoculated intradermally with recombinant vaccinia viruses expressing the H gene of RPV, the F gene of RPV, the H and F genes of RPV, or fowlpox virus recombinant expressing both genes. Two ferrets were vaccinated s.c. with CDV vaccine as positive controls, and 1 group was left unvaccinated as a negative control (Jones et al., 1997).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: The ferrets were challenge exposed with a high dose of CDV (Jones et al., 1997).
Efficacy: Partial protection was seen in all groups, with vRVFH vaccination being the most protective (Jones et al., 1997).

2. rLSDV-RPV

a. Vaccine Ontology ID:

VO_0004763

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Preparation

Recombinant capripox-rinderpest vaccine (Ngichabe et al., 2002).

f. Immunization Route

Intramuscular injection (i.m.)

g. Cattle Response

Vaccination Protocol: A dose of 10^5.3 TCID50/ml of the recombinant vaccine, determined previously as an effective dose, was injected subcutaneously in the shoulder region of each animal (Ngichabe et al., 2002).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: Two years after vaccination, cattle were challenged with virulent RPV (Ngichabe et al., 2002).
Efficacy: In the case of LSDV, all of 4 vaccinated cattle challenged with virulent LSDV at 2 years were completely protected from clinical disease while 2 of 5 vaccinated cattle were completely protected at 3 years. The recombinant vaccine showed no loss of potency when stored lyophylized at 4 degrees C for up to 1 year (Ngichabe et al., 2002).

3. RPV ISCOM Vaccine

a. Type:

Subunit vaccine

b. Status:

Research

c. Host Species for Licensed Use:

None

d. Antigen

H protein (Kamata et al., 2001)

e. Gene Engineering of H protein

Type: Recombinant protein preparation
Description: (Kamata et al., 2001)
Detailed Gene Information: Click here.

f. Preparation

The recombinant baculovirus expressed the RPV H protein as a membrane-bound protein in infected Sf21 insect cells and the protein was purified by solubilizing purified cell membranes with octylglycoside. ISCOMs incorporating the RPV H protein were produced according to a standard method. (Kamata et al., 2001)

g. Immunization Route

subcutaneous injection

h. Description

Rinderpest virus (RPV) ISCOM (Immune-stimulating complex) vaccine induces protection in cattle against virulent RPV challenge. (Kamata et al., 2001)

i. Cattle Response

Vaccination Protocol: Four Friesian cross Aberdeen Angus calves were inoculated subcutaneously with the ISCOM vaccine containing 100 mg RPV H protein in a volume of 1.0 ml. After 5 or 6 weeks, the cattle received the second vaccination with the ISCOM vaccine incorporating 50 mg RPV H protein. Three cattle were used as unvaccinated controls. (Kamata et al., 2001)
Immune Response: Two of the four cattle developed significant levels of neutralizing antibody after the first vaccination, while after the second vaccination, high levels of neutralizing antibody were present in all four animals. Following challenge, a slight increase in antibody titers was observed in the completely protected cattle, whereas a rapid rise in antibody titer was seen in the partially protected animal. The control cattle remained antibody-negative throughout the experiment. (Kamata et al., 2001)
Challenge Protocol: All seven animals were challenged with 104TCID50 of virulent Saudi 1/81 strain of RPV at 25 weeks or 15 weeks after the first vaccination. (Kamata et al., 2001)
Efficacy: The three unvaccinated controls developed severe clinical signs of rinderpest, high fever, severe stomatitis and diarrhea, and were euthanized on either day 9 or 10 following challenge. In contrast, all four vaccinated cattle survived the chal- lenge. Three of the four vaccinated cattle were solidly protected from the disease and showed no clinical signs of infection throughout the experiment. The remaining animal developed a delayed and transient fever and a mild mouth erosion but quickly recovered. (Kamata et al., 2001)

4. v2RVFH

a. Vaccine Ontology ID:

VO_0004813

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Cattle

e. Gene Engineering of RPVgp5 F

Type: Recombinant vector construction
Description: The vaccine v2RVFH is a recombinant vector vaccine that expresses two proteins including the F protein from the rinderpest virus (Verardi et al., 2002).
Detailed Gene Information: Click here.

f. Gene Engineering of RPVgp6 H

Type: Recombinant protein preparation
Description: The vaccine v2RVFH is a recombinant vector vaccine that expresses the H protein from a rinderpest virus (Verardi et al., 2002).
Detailed Gene Information: Click here.

g. Vector:

Vaccinia virus vector (Verardi et al., 2002)

h. Preparation

The v2RVFH vaccine was prepared by using a recombinant vaccinia virus vaccine to expresses both the fusion (F) and hemagglutinin (H) genes of rinderpest virus (RPV) under strong synthetic vaccinia virus promoters (Verardi et al., 2002).

i. Immunization Route

Intramuscular injection (i.m.)

j. Cattle Response

Host Strain: Zebu cattle
Vaccination Protocol: Groups of Zebu cattle (Bos indicus, 2 years old on average) vaccinated intramuscularly (1 ml) with various doses of v2RVFH at the side of the neck. Control groups were also included (Verardi et al., 2002).
Immune Response: Intramuscular vaccination of cattle with 10^8 PFU of v2RVFH provided long-term sterilizing immunity against rinderpest (Verardi et al., 2002).
Side Effects: The vaccine is highly safe. Animals vaccinated with v2RVFH did not develop pock lesions and did not transmit the recombinant vaccinia virus to contact animals (Verardi et al., 2002).
Challenge Protocol: Animals were challenged subcutaneously (1 ml) at the side of the neck with 10^3 to 10^4 TCID50 of the pathogenic Kabete ‘O’ RPV; as little as 1 TCID50 of the virus administered subcutaneously induces clinical rinderpest with 100% mortality in U.S. cattle. Nasal and ocular swabs were taken at 2, 3, 4, and 7 days postchallenge from a group of NVI animals challenged at 4 weeks postvaccination. In addition, prescapular and mesenteric lymph nodes as well as lung, spleen, tonsil, kidney, and heart tissue samples were taken from animals that died following RPV challenge. RPV isolation was attempted from the collected swabs and necropsy samples in primary calf cells (kidney and testis) and Vero cells (Verardi et al., 2002).
Efficacy: Cattle vaccinated intramuscularly with as little as 10^3 PFU of v2RVFH and challenged 1 month later with a lethal dose of RPV were completely protected from clinical disease; the 50% protective dose was determined to be 102 PFU. Animals vaccinated with v2RVFH did not develop pock lesions and did not transmit the recombinant vaccinia virus to contact animals (Verardi et al., 2002).

IV. References

1. Jones et al., 1997: Jones L, Tenorio E, Gorham J, Yilma T. Protective vaccination of ferrets against canine distemper with recombinant pox virus vaccines expressing the H or F genes of rinderpest virus. American journal of veterinary research. 1997; 58(6); 590-593. [PubMed: 9185963].

2. Khandelwal et al., 2004: Khandelwal A, Renukaradhya GJ, Rajasekhar M, Sita GL, Shaila MS. Systemic and oral immunogenicity of hemagglutinin protein of rinderpest virus expressed by transgenic peanut plants in a mouse model. Virology. 2004; 323(2); 284-291. [PubMed: 15193924].

3. Ngichabe et al., 2002: Ngichabe CK, Wamwayi HM, Ndungu EK, Mirangi PK, Bostock CJ, Black DN, Barrett T. Long term immunity in African cattle vaccinated with a recombinant capripox-rinderpest virus vaccine. Epidemiology and infection. 2002; 128(2); 343-349. [PubMed: 12002554].

4. Verardi et al., 2002: Verardi PH, Aziz FH, Ahmad S, Jones LA, Beyene B, Ngotho RN, Wamwayi HM, Yesus MG, Egziabher BG, Yilma TD. Long-term sterilizing immunity to rinderpest in cattle vaccinated with a recombinant vaccinia virus expressing high levels of the fusion and hemagglutinin glycoproteins. Journal of virology. 2002; 76(2); 484-491. [PubMed: 11752138].