Molecule Role Annotation :
IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
Additional Molecule Role :
Vaximmutor
Additional Molecule Role Annotation :
The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
The antigen expressed on S. enterica is the "210-239 amino acid epitope, (designated as Ag30) derived from the 43 kDa glycoprotein of T. spiralis muscle larvae" (Pompa-Mera et al., 2011).
e. Vector:
The strain SL3261 of the bacterium Salmonella enterica serovar Typhimurium is used as a live, attenuated vaccine vector expressing Ag30 from T. spiralis, displayed on the surface of the bacterium by the autotransporter ShdA (Pompa-Mera et al., 2011).
This vaccine was shown to elicit protective immunity against T. spiralis challenge, in the form of the induction of antigen-specific IgG1 and IL-5 production (Pompa-Mera et al., 2011).
i.
Mouse Response
Host Strain:
BALB/c mice
Vaccine Immune Response Type:
VO_0000287
Immune Response:
The mice were shown to have produced antigen-specific IgG1 and IL-5 antibodies in response to T. spiralis challenge.
Efficacy:
At day 8 post-challenge, the adult burden was reduced by 61.83% in mice that had been inoculated with the recombinant vector vaccine (Pompa-Mera et al., 2011).
Description:
The immune response and protection offered by the vaccine shows the usefulness of S. enterica for displaying nematode epitopes found in intestinal parasites (Pompa-Mera et al., 2011).
V. References
1. Liu et al., 2017: Liu CY, Song YY, Ren HN, Sun GG, Liu RD, Jiang P, Long SR, Zhang X, Wang ZQ, Cui J. Cloning and expression of a Trichinella spiralis putative glutathione S-transferase and its elicited protective immunity against challenge infections. Parasites & vectors. 2017; 10(1); 448. [PubMed: 28962639].
3. Yang et al., 2018: Yang Z, Li W, Yang Z, Pan A, Liao W, Zhou X. A novel antigenic cathepsin B protease induces protective immunity in Trichinella-infected mice. Vaccine. 2018; 36(2); 248-255. [PubMed: 29199042].
