VIOLIN: Vaccine Investigation and Online Information Network

Schistosoma japonicum

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. Ak1 (Protective antigen)
2. CP1412 (Protective antigen)
3. FABP (Protective antigen)
4. Il12a (Protective antigen)
5. Paramyosin (Protective antigen)
6. Sj23 (Protective antigen)
7. TPI (Protective antigen)
8. TSP (Protective antigen)
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

6182

2. Disease:

Schistosomiasis

3. Introduction

Schistosoma japonica remains endemic in seven provinces in China. Human infection is acquired during the course of domestic or occupational activities, in particular fishing and farming. Acute infection may result in fever, weakness, diarrhoea, abdominal pain and hepatomegaly. Chronic disease involves granuloma formation, tissue inflammation, liver lesions and fibrosis, which may persist after infection has been cleared. Schistosoma japonicum is also known to infect 45 species of animals, of which water buffaloes are especially important for transmission (Balen et al., 2007).

II. Vaccine Related Pathogen Genes

1. Ak1

Gene Name : Ak1
Sequence Strain (Species/Organism) : Schistosoma japonicum
NCBI Protein GI : CAX82987
Other Database IDs : CDD:130427
CDD:238713
GOA:Q86BY2
HSSP: 3ADK
InterPro: IPR000850
UniProtKB/TrEMBL: Q86BY2
Taxonomy ID : 6182
Protein Name : adenylate kinase 1
Protein pI : 8.37
Protein Weight : 21124.542
Protein Length : 256
Protein Note : complete sequence of full-length cDNA

Protein Sequence : Show Sequence

>CAX82987.1 adenylate kinase 1 [Schistosoma japonicum]
MTDQKLAKAKVIFVLGGPGSGKGTQCEKLVHKFNFNHLSSGDLLRAEVQSGSQKGKELKAMMERGELVPL
EVVLSLLKEAMIKLVDKNCHFLIDGYPRELDQGLKFEKEVCPCLCVINFDVSEEVMRKRLLKRAETSNRV
DDNEETIVKRFRTFNELTKPVIEYYKKQNKVITIDASGTVDDIFEKLTHELQKFGIK

Molecule Role : Protective antigen
Molecule Role Annotation : (Gao et al., 2017)

2. CP1412

Gene Name : CP1412
Sequence Strain (Species/Organism) : Schistosoma japonicum
NCBI Protein GI : AAS68246
Other Database IDs : CDD:238512
Taxonomy ID : 6182
Protein Name : egg protein CP1412
Protein pI : 6.72
Protein Weight : 26644.42
Protein Length : 300
Protein Note : Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf...; cd01061

Protein Sequence : Show Sequence

>AAS68246.1 egg protein CP1412 [Schistosoma japonicum]
MYPLLICLIVLSSMITLKSQSVQDNSWDRFVFEIIWTPSVCNHSVKCNPPEGFDHFTIVGLWPVTSSGSR
PKCTTIVNFNMSEIEDLRGELLTYWPYYRDVKTSENKWRIEYEINGPCAIEGPIILSERDYFSYSIAQLK
NMDILKTLWSHGIMPSDTIPQNKTSFQNALELEYKVNVTLMCTRSSDQAKKKSLERIVICLTRDFTFTQC
PPGLGGRPVECPPQFMFPAYKQLTQLSTSV

Molecule Role : Protective antigen
Molecule Role Annotation : (Ke et al., 2017)

3. FABP

Gene Name : FABP
Sequence Strain (Species/Organism) : Schistosoma japonicum
NCBI Protein GI : 349804
Other Database IDs : CDD:278490
Taxonomy ID : 6182
Gene Strand (Orientation) : ?
Protein Name : fatty acid binding protein
Protein pI : 9.01
Protein Weight : 13671.32
Protein Length : 198
Protein Note : Lipocalin / cytosolic fatty-acid binding protein family; pfam00061

Protein Sequence : Show Sequence

>AAA64426.1 fatty acid binding protein [Schistosoma japonicum]
MSSFLGKWKLSESHNFDAVMSKLGVSWATRQIGNTVTPTVTFTMDGDTMTMLTESTFKNLSVTFKFGEEF
DEKTSDGRSVKSVVTKDSESKITHTQKDSKNTTVIVREIVGDTMKTTVTVDDVTAIRNYKRL

Molecule Role : Protective antigen
Related Vaccine(s): Chinese S. japonicum DNA vaccine pVAX/SjFABP encoding FABP

4. Il12a

Gene Name : Il12a
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16159
NCBI Protein GI : 226874945
Genbank Accession : AC121306
Protein Accession : NP_001152896
Taxonomy ID : 10090
Chromosome No : 3
Gene Starting Position : 68690643
Gene Ending Position : 68698549
Gene Strand (Orientation) : +
Protein Name : transcript variant 1
Protein pI : 8.33
Protein Weight : 25111.9
Protein Length : 236
Protein Note : Also known as p35; Ll12a; Il-12a; IL-12p35

DNA Sequence : Show Sequence

>NC_000069.6:68690643-68698549 Mus musculus strain C57BL/6J chromosome 3, GRCm38.p4 C57BL/6J
CTGCCACCTACTCCCTTGGATCTGAGCTGGACCCTTGCATCTGGCGTCTACACTGCTGCTGAAATCTTCT
CACCGTGCACATCCAAGGATATCTCTATGGTAAAAGTCCAGAAAGGCTAAAGATGTTGTCCACAATCTCT
CGGCAATGACCAGATATATCCCCACCAGTATCACAACTTGCAACGCGCTCCGTTCCCATCTGCCCTCTGC
CTCCCTACTGTGTTGATTCCCATTTCCAAAACTAGCTCAGAATGCATCCCACTGGCAGCCAGGCAATGCC
TTCTTGCAGATCTGGAGGCTTCAGGAGCCTTTGCTGCCTCTTAGGCTCCTGCAGAGAGATGGCCTTGCTC
TCTCTGGGCCCTTTTCCCCCAAGATGCTGTGCAGTCGTCTCACTAAATCAAAACTGCCAGAACTGAGTTT
TAAGCGATATATAGCCACCACATCTATTTTGTTCCTTTATACTTTCAAAGTAGGACAGATTCGCAAAATA
CATTGAGTCAGACTCAGTGTCCACGATGGAGAGACGAGTGGGTAGCTTGTGCTTCTTTAGAAGGGGCACT
GGGCAGCGTGTTAGGGCTAGGGGGTAGTGGGGCAGGACAGACCTGTCCCGGGACAAGAGTGGCTACTCGC
AGGCCGGTTTTGCTTTCGCTCTGAGTGTGGTGGGCGGTGGGACGGTCCCGAATCTCCTCAAACGGGCACT
CCGAGAGTTAATTCGAAAGCGCCACCAGCCTTGGGGGCGATCGACGCACTTGTCCTTGAGATGTAGAGAC
AGAGAGAGAAAGCAAGAGACACAGTCCTGGGAAAGTCCTGCCGGCTATCCAGACAATTATAAAAATGTGT
CTCCCAAGGTCAGCGTTCCAACAGCCTCACCCTCGGCATCCAGCAGCTCCTCTCAGTGCCGGTCCAGCAT
GTGTCAATCACGCTGTAAGTACCTGAGGGCCGGGACCAGGGTGACTTGATGAGAGGAGAAGGCTTGAGAA
AGAACAGACAGAAGCCCTGGACCCATTAAGGGAGACTAAAGGGCTGCCAGCAACTGCAGGCCAAGGGAAG
GAAGAGGGGAAAGATGGTCGTGCTAGATTGCGCTGCACCGAGCATGGTCTCATTATAGAACAACCCTGGG
GGCTACGGCTATGCCCGTTTCACAGAAGAGAACTAAAGCCCAGCCTTGAAACCAATGTAAGGAAAATGCA
GGTCTCAGGCAGTCCCCAAACAAGATCTGGGAGGTCTTGCTTCATCTGTCAGCCCCGGGGAAGGGCTTAA
ATTATGTGTGGAGGGAGGTCAGTACCCAGAATGGATGGAAAAAATTGCCAGAATGGCTGAAGAAAGAGGC
AGGTCAAAACCAAGTTGACAAGCTCCACCTGCCCTTTGTTTCCCTTCCAGACCTCCTCTTTTTGGCCACC
CTTGCCCTCCTAAACCACCTCAGTTTGGCCAGGGTCATTCCAGTCTCTGGACCTGCCAGGTGTCTTAGCC
AGTCCCGAAACCTGCTGAAGACCACAGATGACATGGTGAAGACGGTGAGTTTCTCCTGTCCTTCTGGCTT
CAAAGTTCCGCTGTTGACATGACTTCTAACCTATCTGGTATCTGCTGGGAAGTTTATGGTATCTGGCAGG
AACCGTAGAGGGTTTCAAGACTTCTAAATATAATCTAAAGGAAACTGTGTCTCTAGAGAAAGGAAGAATG
GTAATAAATTGTAACGGCATCGGTTTAGGCTGTGTCTACACAGAGGAAAGTGGGGACTTGCAATGTCCCT
CCCAGCCTGCCTGCCTGTGTGACACAGTGTCAGGTGCTCCCTGCTTCTGCTTCAGGGCAGGTGGAACAGG
GAGACAGTCTCTAGGTACACTCTCTGTGCTTCACAAGCCATAGAGATGAATTAGATTGTCTCTCCCCCTG
CTCACCAGTGTCCAGCACTGTGATGCAGTCCCTTGTCGGATGGACACATGTCAGGGAGGACTCAGACATC
AGATAGCAAGCAGGTCCAGCCTCCAGTTTTCTGGGGCAAGTGTGATCCAGTTTTAGTCACGGCTCCACTC
ATTTTTCTCAGGTTTCATGTTTGAATGCAAAATAGCCTTTTCCGTTGTAAAATTGCCACGGTTTCCTCTC
GCTTTGGGGACAAAGCTACAGCAAAATGCTAATGTGTTTGAGATATCCTGCAGCTGCTAGGGCTGGAGAT
CCAGTAGAATTCTGGCTTAATTATGAATGGGTCTCCCGGGTTCTATCCCCCAACAGAGCGTGCGTTCATA
AGCACCTGGGAACAGAGGAGGGAAGGAGGGAAACTCTGTCTTCCTGTTTTGTTTTTAAATCTTTCTTGAG
GATCTTCTAGCTTGTTGTCTTATGGCTATCCCAACATAAGCTCCGCTCCACAAGCCCTTCTTGGTGAATT
TTCTTTCTTTCCTTCCCGTCTTGCATAACGTGCTGCTGGCTGTTCCTCCAAGCTCAGTCTTAATTGAGTG
GGGTTCCCAGGAGGGAGAGATGGCTCTGTGGTTAAGAGAGCTGGCTGCTCTTCCAGAGGTCCAGAGTTTC
CACACCCTCATGGTGGCTCACAATCATGGTAACTCTGATCATAGGGAACCTGTCGTTTTCTCTGTGCCTC
TGTGGGCACTGAGCACATGCCACACAGACATCCATGCAGGCAGAACACTCTTGGCCATGAAACCTTAAAA
TGAAGGAGAAACCTTACGAGAGGTTCCCTAAGGAATCCCTGCAGAAGGGAGAGATTGTTTTCCTAAGATG
AGGTTTGGTGTGGAGGGGATGAGGTATTCAAAGTTAACTAGCCGCTCCTCACTCCTCTGCTGCCAAATAC
CAGCACTGGGCTTCGAGACACAATAGAATTCGACCTTGTTTTCGTTTGGGTCACTATTGCTCTCTAGTTG
AACACGGAATCCACGGGGGCACATTGCTGTCTGTCCTTTGTCACACCCCAGGACCACAGCTGCATGGAAA
AGTCATCGCAGGCTTCAGTCAGAGAGCTGTTGACTTCCTAATATGTTGTGTAATTCTGTTCAGTGGTGCT
TTCCAGCACACCAGGGAATAAGAGACAGTCCCCGCGTATGTAAATTAAAGCTCAACTAGTATTTAGGGCT
TGCAAGTTTGTTACAGCCATCAAGCTGTGACTTTAACAGTTTCCTCTTTAGAAGTTAAACTTGAAAAAAA
ATAGAGGGAGAGAGAGATTCACAGGGCACAGCTGCTTGGAAAAAGGAAAAGCCACAGGACTCACCAGAAG
CAAGCAGCACAGTGTGGCACTGATGCTGATGAGTACAGCCTGCTGCACCCCAGTGTTCACATGGTGAAAA
AAATACCCTGCTGGCGGACAGCCTAGGCAAAGGGGGAGGAGAGTAGGGAGAGCGTGCGGGAGGTTGGGTT
TCTTAGCTTATGAAGAGCCTGAGTTTCCTACAGGCCAAAGAGATGACGGTTCAGAGTGGCCAGCCTAGGC
TCTGCTGATCCACTGCGATGCAGGTCTCAGAGGGAACCAGCTGTCTTGCTGAGAGATTCACCTCAGCTTC
TCCTTATGTTTTTCCCAGGCCAGAGAAAAACTGAAACATTATTCCTGCACTGCTGAAGACATCGATCATG
AAGACATCACACGGGACCAAACCAGCACATTGAAGACCTGTTTACCACTGGAACTACACAAGGTATGGAG
GACCCCCACCCAAAAAACAACAACAACAACAACAAAAAACACAATTAAGAGCCGTGCAGCCAACCTCAGC
TGTCTCCAGATACTTACTTGTAGCTATCAAACGTGAGAGTTTTGAAGAAAAAAAAAAAAGTCAACAACCA
TACAAATACTTCTACAAGCTGAAGGATATACTCGGTAACTAGGAGGTGGCTATGGTGGTATAGACCTTTA
ATTCCAGCACTTGATGGATAGAGGAAGGCCGGCTCCTGTTAAGTTAGAGGCCAGTCAGGGATACACAGCA
AGATTTCATCTTTAAAAAGAAAGGGGGTGGTGTGGCGGGTGAAGCTTACTTATTGAAGTGCTCATGTCAC
AAGCCTGGGGACCTAAGTTCCCATCCCCAGCACCCATGTAGGAGCTGGGTGTGATGGTTTCACATGCAGA
GACAGGAGGATACCTGGGCCAGCTAGTCTAGCCGAATCCATGAGCTGCAGGCCCAGTGAGAGAACCTGTA
TCAGAAAACAAGAAAGTCAGGAGAGCGGCTGAGAAAGACCCCAAAGATCATCCTCCGATTTCCACGAGTA
CATGCACCTGCACACACGTGCACACAGGAATCCTATACATAGCCACCCAAAAGCAGTTATGATGGTGAAG
TTTCTTTTACTGGAATGAAAAGTTAGTTTGAGAAAAAAATATCAGAGAGATTTATATTAAGAGTGAATGG
GTGGGGGGTATGCATGCCTTTAATCTCAGCACTTGATAGGCAGAGGCAGATGGATCTCTGTGAGTTCAAG
GCCGGCCTGGTCTACAGAGTAAGTTCCAGGACAGCCAGTGCTACAATAGAAACAGGTGATGGTGATGATG
GTGATGATGGCGGTGATGATGATGATGATGACGAAGACAAACTCCTCGGTTTATGATTTTCTTCTAGAAC
GAGAGTTGCCTGGCTACTAGAGAGACTTCTTCCACAACAGTAAGTAAGCACTCTAAGGGTTCCTTCCCCA
TGACGGATTCATAACACTGATGCCTGGTCATTCTTTCTCTAGAGAGGGAGCTGCCTGCCCCCACAGAAGA
CGTCTTTGATGATGGTAAGCCACATCACTTTTCTGAAATGCAGCAGGGTCCTGTAGTTCCAGCCCATCTC
AATGATTCCTTGCCCATTGTGTCCTGCGCTCTAGACCCTGTGCCTTGGTAGCATCTATGAGGACTTGAAG
ATGTACCAGACAGAGTTCCAGGCCATCAACGCAGCACTTCAGAATCACAACCATCAGCAGATCATTCTAG
ACAAGGGCATGCTGGTGGCCATCGATGAGCTGATGCAGGTAAGCCTTCCTCCTATCAGCCAGCGCTCCTT
TTCCATGGTGAAGAGAGGTCCTTTATTAGATATGATAAAATCAGCACAGCTTGGGTTGGGAGAGACCTGA
ACACCCCCACCCCACCCCACACCCTCATCCCCCAACCATGAAAGGCCTTGCAAGGTTACATTTGCTTAGA
GAGCCTTGAGGGGGCCTCTACAATAAGGGCAAATGCAAGCAGCCTTCTACTTGTTGACTCATGATACAAA
TCAGGCACTTTTCAAATGCTTCTTCTCATACCATCCCCACAACAAGCAAATAAGGTCTCTACAGAGGAGG
AAACTGAAGCTTAAGAGGATGAACGAGGGTTCCCTACAAAAGGGTTTTTTGTTGTTTGGTTTGGTTTGGT
TTGGTTTGGTTTGGTTTGGTTTTTCCGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTCAC
TTTGTAGATCAGGCTGGCCTCGAACTCAGAACTCCACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGG
TGTGCGCCACCATTGCCCAGCTTATTGTTGTTTTAAATACATTCTTTTCTTAAAGGTTCAAGATTAGGAC
TGGAGAGACAGTCCGATGATTAAGAGGACTTGAGTTTGGCCTGGGGCTCCTATGTAACAAACAGGGTGTG
GTCCTGAACTGCTCTCTAACTCCAGAGAGTGGAGACAGGAGGCCTATGGAGGCTTTCTTGCTGCAAGTTT
AGCCATGAAATCTCCAGGTTCAGGGAGAAAGCATGCCTCAAAGAAATAAGATGGAGAGTCACGTGATCTC
TTCTCTGGCTTCTGCATGGTGTAGGCACAGATATGCACATGTTTGAGTGCATACAATACAGGCAAACATA
CACGTGTCCTTTTTAAAATGTATAGGATGGACATTCTAGGGGCTGCACACATATTCAAATATTACTAGGG
GAAGCACTTGGGGCTGGTGTCCAAGTATAATCTATTTTGAACCAAAATGTGACCAAAATGAAAAGTGGTC
TTACATCTAGGTAATTACAGTGTTAGGTATCCGTGGGAACTTTGCTGTACCACCAGTAACACAAAGCACA
GGAGTGTGCACACCAGAGACAAGCACCTTGGCAACCAAGGCGTGGGATGAACATATGTTCAGAAGTCACT
GCCTGGAAAGCAATTGTTTTTCATTTCATACATACTGTCTTGCACAGGGTTTCTATGTCTTAGATAAAAT
ATCATGACGGAAAGCAAGTTGGGGAGGAAAGGGTTTATTTCATCTTGTAGAGTGTAGTGAGTCTATTACC
CAGGGAAGTGGAGGCAGAAGCTGATGCTGGAGAGGACTCCTGGTGACTGGAGTGCTCCACATGACTTGCT
CAGTGTGCTTTGTATAGCACCCAGGACCACCAGGCTAGGGTGAGCTGGTGCCTCCCACATCAACTGTCAA
TCAAGAAAATCACCAGAGGCTTGCCCACTGGCCAATCTGGCTGGAGGTGTTTTTCTCAATGACCACAGGC
TTGTGTAGAGTTAACATAAAAGCACCCAGGACATTACATATTGCCAGATGACTCCTTTGAAAGACAGTGA
AGTTTATGCAGGTCAAACAACCTATCATGTAGAGATTTGTAATGGATATTGCATGAACAAGGCCAGAAGT
CAGGTGATTTGGATAGTAGGAAGAGGTCTACCAGGGACCAGCCAACTATGCTGACAGGGTTAACTCTTGT
CAGCCTGTTTGTAAAATAAGATGACTTTAAATAGCCTATGTCTCTCTTGAGATTTTTGTGAGGAGCAAGC
AAAAATCTTAAACATCACAGAAACCTAAGTTCTTATTCTATGTTATGAAATCAAAGGATCGAGTGGGGAA
ACACGGTACTGGCTTGTTTATCTACCACAAATAGCGTTTCTATCAGAGATAGGTCTGTTGTTGATAATAA
GCTCTAATCCATAGATCCTACTTACCCTCCAACTCCCTCCTCTAAAAGAACAGGACTTCTTAACATAATT
TATAAAAATTAATTAGCTATTGCTGCAGGTGCTACCTCATAGACTCACACCTTCTGAGAGATCAGCTCTA
ATGACAATTTCTTTCTGGAAAATTTAGGGGTCTTGTCAGAGATCATTAAAAACATAGGTATATGCTCACA
AAAGAGTATTTCTTCCCTCTTTTCCTGCAGGAACACAGCAGGAGTAGATGTGAAATGGGTCTAGGAGTGT
GATGAGGGGCTAGGTTGCAGGCCCTGGACTTTGCATTGACTGTCTCCCATTTTGCAGACAAACATTCTAA
TTTTTAAATTGTGCATATGCTATGCTTGGTGTTTCTTTCTAGTCTCTGAATCATAATGGCGAGACTCTGC
GCCAGAAACCTCCTGTGGGAGAAGCAGACCCTTACAGAGTGAAAATGAAGCTCTGCATCCTGCTTCACGC
CTTCAGCACCCGCGTCGTGACCATCAACAGGGTGATGGGCTATCTGAGCTCCGCCTGAAAGGCTCAAGGC
CCTCTGCCACAGCGCCCTCCTCACACAGATAGGAAACAAAGAAAGATTCATAAGAGTCAGGTGGTCTTGG
CCTGGTGGGCCTTAAGCTCCTTCAGGAATCTGTTCTCCCATCACATCTCATCTCCCCAAAGGTGGCACAG
CTACCTCAGCATGGTCCCCTCCATCGCTTCTCTCATATTCACTATACAAGTTGTTTGTAAGTTTTCATCA
AAATATTGTTAAGGGGCGAAGACGTCCTCCCCTCAATGTGTTAGCAGAAGAGCAAGAACTGATAAGCTAT
TGTTTTTGTGCCAAAGTGTTTATGAAAACACTCAGTCACCCCTTATTTAAAAATATTTATTGCTATATTT
TATACTCATGAAAGTACATGAGCCTATTTATATTTATTTATTTTCTATTTATTATAATATTTCTTATCAG
ATGAATTTGAAACATTTTGAAACATACCTTATTTTGTGGTTCTAATAAAGTAATGTTATCACTTCAC

Protein Sequence : Show Sequence

>NP_001152896.1 interleukin-12 subunit alpha isoform 1 [Mus musculus]
MVSVPTASPSASSSSSQCRSSMCQSRYLLFLATLALLNHLSLARVIPVSGPARCLSQSRNLLKTTDDMVK
TAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLC
LGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKM
KLCILLHAFSTRVVTINRVMGYLSSA

Molecule Role : Protective antigen
Related Vaccine(s): Chinese S. japonicum DNA vaccine pVIVO2-IL12-Sj23 encoding Sj23 and IL-12

5. Paramyosin

Gene Name : Paramyosin
Sequence Strain (Species/Organism) : Schistosoma japonicum
NCBI Protein GI : 4761226
Other Database IDs : CDD:278467
CDD:283846
Taxonomy ID : 6182
Gene Strand (Orientation) : ?
Protein Name : paramyosin
Protein pI : 5.01
Protein Weight : 97539.21
Protein Length : 927
Protein Note : Intermediate filament protein; pfam00038

Protein Sequence : Show Sequence

>AAD29285.1 paramyosin [Schistosoma japonicum]
MMNHDTESHVKISRTIYRGVSPSTTRLESRVRELEDILDLERDARVRAERHAADLSYQVDALSERLDEAG
GSTTQTQELLKRREMEINKLRKDLENANASLELAETSMRRRHQTALNELSLEVENLQKQKGKAEKDKSHL
IMEVDNVLGQLDGALKAKQSAESKLEGLDSQLNRLKTLTDDLQRQLTELNNAKSRLTSENFELLHINQDY
EAQILNYSKAKSSLESQVDDLKRSLDDESRNRFNLQAQLTSLQMDYDNLQAKYDEESEEASNLRNQVSKF
NADIAALKSKFERELMSKTEEFEEMKRKLTMRITELEDVAERERLKAVSLEKLKTKLTLEIKDLQSEIES
LSLENGELIRRAKSAESLASELQRRVDELTIEVNTLTSQNNQLESENMRLKSLVNDLTDKNNALERENRQ
MNDQVKELKSSLRDANRRLTDLEALRSQLEAERDNLASALHDAEEALRDMDQKYQASQAALNHLKSEMEQ
RLRERDEELESLRKSTTRTIEELTVTITEMEVKYKSELSRLKKRYESSIADLEIQLDATNKANANLMKEN
KNLAQRIKDLETFLDDERRLREAAENNLQITEHKRIQLANEVEELRSAMENLERLRKHAETELEETQSRV
SELTIQVNTLSNDKRRLEGDIGVMQADMDDAINAKQAAEDRATRLNNEVLRLADELRQEQENYKHAEALR
KQLEIEIREITVKLEEAEAYATREGRRMVQKLQARVRELEAEFDGESRRCKDALAQARKFERQYKELQTQ
AEDDRRMVLELQDLLDKTQMKMKAYKRQLEEMEEVSQITMNKYRKAQQQIEEAEHRADMAERTVTVRRVG
PGGRAVSVARELSVTSNRGMRATSMM

Molecule Role : Protective antigen
Related Vaccine(s): Chinese S. japonicum DNA vaccine pCMV-Sjc97 encoding paramyosin

6. Sj23

Gene Name : Sj23
Sequence Strain (Species/Organism) : Schistosoma japonicum
NCBI Protein GI : 124390
Other Database IDs : CDD:278750
CDD:239401
Taxonomy ID : 6182
Gene Strand (Orientation) : ?
Protein Name : 23 kDa integral membrane protein
Protein pI : 6.08
Protein Weight : 22840.74
Protein Length : 314
Protein Note : Sj23

Protein Sequence : Show Sequence

>sp|P27591.1|IM23_SCHJA RecName: Full=23 kDa integral membrane protein; AltName: Full=Sj23
MATLGTGMRCLKSCVFILNIICLLCSLVLIGAGAYVEVKFSQYEANLHKVWQAAPIAIIVVGVVILIVSF
LGCCGAIKENVCMLYMYAFFLIVLLIAELVAAIVAVVYKDKIDDEINTLMTGALENPNEEITATMDKIQT
SFHCCGVKGPDDYKGNVPASCKEGQEVYVQGCLSVFSAFLKRNLIIVACVAFGVCFFQLLSIVIACCLGQ
RIHDYQNV

Molecule Role : Protective antigen
Related Vaccine(s): Chinese S. japonicum DNA vaccine pVIVO2-IL12-Sj23 encoding Sj23 and IL-12 , Chinese S. japonicum DNA vaccine SjC23 , Chinese S. japonicum DNA vaccine SjC23-Hsp70

7. TPI

Gene Name : TPI
Sequence Strain (Species/Organism) : Schistosoma japonicum
NCBI Protein GI : 2501312
Other Database IDs : CDD:238190
Taxonomy ID : 6182
Gene Strand (Orientation) : ?
Protein Name : Triosephosphate isomerase
Protein pI : 7.47
Protein Weight : 27589.91
Protein Length : 374
Protein Note : TIM; Triose-phosphate isomerase

Protein Sequence : Show Sequence

>sp|Q27775.1|TPIS_SCHJA RecName: Full=Triosephosphate isomerase; Short=TIM; AltName: Full=Triose-phosphate isomerase
MSGSRKFFVGGNWKMNGSRADNKVLIHSLSEAHFCGDTEILIAAPFVYLNEVRQSLAKEIHVAAQNCYKV
PKGAFTGEISPSMIKDVGCDWVILGHSERRSIFNESDELVAEKVQHALAEGLSVVACIGETLSERESGKT
EEVCVRQLNAIANKIKSIDEWKRVVVAYEPVWAIGTGKVASPGQAQEVHNFLRKWFKTNTPSGVDQQIRI
IYGGSVTAANCKELAQQHDVDGFLVGGASLKPEFIDICKARQ

Molecule Role : Protective antigen
Related Vaccine(s): Chinese S. japonicum DNA vaccine SjCTPI , Chinese S. japonicum DNA vaccine SjCTPI-Hsp70

8. TSP

Gene Name : TSP
Sequence Strain (Species/Organism) : Schistosoma japonicum
NCBI Protein GI : ABQ44513
Other Database IDs : CDD:278750
CDD:239401
Taxonomy ID : 6182
Protein Name : TSP
Protein pI : 7.76
Protein Weight : 22314.55
Protein Length : 260
Protein Note : Tetraspanin family; pfam00335

Protein Sequence : Show Sequence

>ABQ44513.1 TSP [Schistosoma japonicum]
MALGCGYKCLQCLLIIFNSGVFICGIGLIVVGALGLHSTVNHWKELDPPLQSLIIFIIALGCFLFVLGAL
GMFGACTKNVCLLTTYCILLSILIIAQIAAGIYAIIEKPKVKRHVTTALKDLVGQYGHDRHLDKVLDEIQ
DKLQCCGAESPADYSRGPPPSCKNYNEGCIGKVTDLTKKHLNATIVTVFIFALLQMICLVFAVCVLMAIK
RGDDE

Molecule Role : Protective antigen
Molecule Role Annotation : (Chen et al., 2016)

III. Vaccine Information

1. Chinese S. japonicum DNA vaccine pCMV-Sjc97 encoding paramyosin

a. Vaccine Ontology ID:

VO_0004456

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of Paramyosin

Type: DNA vaccine construction
Description: Vector pcDNA vector/Amp expressed the full-length cDNA encoding paramyosin of Chinese S. japonicum (Sjc97) (Zhou et al., 2000).
Detailed Gene Information: Click here.

f. Vector:

pcDNA1/Amp (Zhou et al., 2000)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Efficacy: In three separate experiments, pCMV-Sjc97, inoculated intramuscularly three times at doses of 100 or 200 μg per C57BL/6 mouse, was found to provide significant worm reduction rates. In addition, there was a substantial reduction in the number of eggs present in the livers and spleens of the pCMV-Sjc97 immunized group when compared with the pCMV blank vector control groups. Therefore, preliminary results suggest pCMV-Sjc97 can confer protective immunity in C57BL/6 mice (Zhou et al., 2000).

2. Chinese S. japonicum DNA vaccine pVAX/SjFABP encoding FABP

a. Vaccine Ontology ID:

VO_0004457

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of FABP

Type: DNA vaccine construction
Description: Vector pVAX1 expressed Schistosoma japonicum 14 kDa fatty acid binding protein (SjFABP) (Wei et al., 2009).
Detailed Gene Information: Click here.

f. Vector:

pVAX1 (Wei et al., 2009)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Efficacy: The mice inoculated intramuscularly with pVAX/SjFABP showed significant worm reduction rate of 22.94% (P < 0.05) and egg reduction rate of 30.23% when compared with the pVAX1 control group. The addition of the pVAX/mIL-18 plasmids dramatically enhanced protection with worm reduction rate of 36.92% and egg reduction rate of 43.23% (P < 0.05), compared to the pVAX/SjFABP group (Wei et al., 2009).

3. Chinese S. japonicum DNA vaccine pVIVO2-IL12-Sj23 encoding Sj23 and IL-12

a. Vaccine Ontology ID:

VO_0004460

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of Sj23

Type: DNA vaccine construction
Description: Vector pVIVO2 co-expressed S. japonicum integral membrane protein Sj23 and murine cytokine IL-12 (Gan et al., 2004).
Detailed Gene Information: Click here.

f. Gene Engineering of Il12a

Type: DNA vaccine construction
Description: Vector pVIVO2 co-expressed S. japonicum integral membrane protein Sj23 and murine cytokine IL-12 (Gan et al., 2004).
Detailed Gene Information: Click here.

g. Vector:

pVIVO2 (Gan et al., 2004)

h. Immunization Route

Intramuscular injection (i.m.)

i. Mouse Response

Vaccine Immune Response Type: VO_0000286
Efficacy: A 45.53% reduction in worm burden as well as a 58.35% egg reduction rate were found in mice vaccinated with pVIVO2-IL12-Sj23. The multivalent DNA vaccine pVIVO2-IL12-Sj23 induced stronger protection than the monovalent vaccine pVIVO2-Sj23 (P<0.05) (Gan et al., 2004).

4. Chinese S. japonicum DNA vaccine SjC23

a. Vaccine Ontology ID:

VO_0004512

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Water buffalo

e. Gene Engineering of Sj23

Type: DNA vaccine construction
Description: pVAX1 expressed SjC23 (Da'dara et al., 2008).
Detailed Gene Information: Click here.

f. Vector:

pVAX1 (Da'dara et al., 2008)

g. Immunization Route

Intramuscular injection (i.m.)

h. Water buffalo Response

Vaccine Immune Response Type: VO_0000286
Efficacy: This vaccine reduced worm burdens by 45.5% and fecal miracidial hatching by 47.4% after challenge (Da'dara et al., 2008).

5. Chinese S. japonicum DNA vaccine SjC23-Hsp70

a. Vaccine Ontology ID:

VO_0004511

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Water buffalo

e. Gene Engineering of Sj23

Type: DNA vaccine construction
Description: pVAX1 expressed SjC23 and bovine heat shock protein 70 (Da'dara et al., 2008).
Detailed Gene Information: Click here.

f. Vector:

pVAX1 (Da'dara et al., 2008)

g. Immunization Route

Intramuscular injection (i.m.)

h. Water buffalo Response

Vaccine Immune Response Type: VO_0000286
Efficacy: Worm burdens were reduced by 50.9% and fecal miracidial hatching by 52.0% after challenge (Da'dara et al., 2008).

6. Chinese S. japonicum DNA vaccine SjCTPI

a. Vaccine Ontology ID:

VO_0004458

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Water buffalo

e. Gene Engineering of TPI

Type: DNA vaccine construction
Description: This DNA vaccine expressed triose-phosphate isomerase (SjCTPI) (Da'dara et al., 2008).
Detailed Gene Information: Click here.

f. Immunization Route

Intramuscular injection (i.m.)

g. Water buffalo Response

Vaccine Immune Response Type: VO_0000286
Efficacy: Worm burdens were reduced by 41.5% and fecal miracidial hatching were reduced by 33.2% (Da'dara et al., 2008).

7. Chinese S. japonicum DNA vaccine SjCTPI-Hsp70

a. Vaccine Ontology ID:

VO_0004459

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Water buffalo

e. Gene Engineering of TPI

Type: DNA vaccine construction
Description: This DNA vaccine expressed triose-phosphate isomerase (SjCTPI) fused to bovine heat shock protein 70 (Da'dara et al., 2008).
Detailed Gene Information: Click here.

f. Immunization Route

Intramuscular injection (i.m.)

g. Water buffalo Response

Vaccine Immune Response Type: VO_0000286
Efficacy: This vaccine produced a 51.2% reduction in worm burden, 61.5% liver egg reduction, 52.1% fecal egg reduction and 52.1% reduction in fecal miracidia (Da'dara et al., 2008).

8. Chinese S. japonicum M6-Sj-F1 Vaccine Vector

a. Type:

Recombinant vector vaccine

b. Status:

Research

c. Antigen

The Sj-F1 antigen of S. japonicum (Wang et al., 2013).

d. Vector:

The bacterium Streptococcus gordonii is used as the vaccine vector, with the Streptococcus pyogenes protein M6 designed to surface-localize the S. japonicum antigen on S. gordonii (Wang et al., 2013).

e. Immunization Route

intranasal immunization

f. Description

Because chemotherapy is currently the main effective method to treat schistosomiasis, but it doesn't prevent reinfection, there has been a need to create a vaccine that can safely express the pathogenic antigen and elicit protection against the disease. This vectored vaccine has been shown to partially protect laboratory mice from S. japonicum challenge, making it a good candidate for schistosomiasis prevention (Wang et al., 2013).

g. Mouse Response

Vaccine Immune Response Type: VO_0000287
Immune Response: The mice inoculated with the vaccine showed strong serum IgG, serum IgA, and saliva IgA antibodies against the antigen Sj-F1 (Wang et al., 2013).
Challenge Protocol: The mice were challenged with the cercariae (parasitic larva) of S. japonicum (Wang et al., 2013).
Efficacy: The mice immunized with the vaccine showed "significant protection" including a 21.45% reduction in the number of parasitic worms present and a 34.77% reduction in the number of eggs (Wang et al., 2013).
Description: The protection received shows that the S. gordonii vector expressing S. japonicum is highly immunogenic and has potential to become a safe, effective vaccine against schistosomiasis (Wang et al., 2013).

9. rAdV-SjTPI.opt

a. Vaccine Ontology ID:

VO_0004782

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Preparation

Replication-defective adenoviral vector-based vaccine with optimized SjTPI (rAdV-SjTPI.opt) (Dai et al., 2014).

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Vaccination Protocol: Mice were randomly divided into different vaccination groups (16 mice/group) for intramuscular (i.m.), subcutaneous (s.c.) and oral immunization (o.i.). Groups consisted of vaccination with the adenoviral vector alone or without vaccination were used as vector or normal control, respectively (Dai et al., 2014).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: Each mouse was challenged with 40 ± 1 S. japonicum cercariae by abdominal skin penetration two weeks after the last vaccination (Dai et al., 2014).
Efficacy: Results showed that intramuscular rAdV-SjTPI.opt induced Th1 biased immune responses in the host, while subcutaneous rAdV-SjTPI.opt induced Th2 predominant immune responses. Oral rAdV-SjTPI.opt induced low levels of immune responses and no significant protection. Intramuscular rAdV-SjTPI.opt provided a consistent and repeatable higher protective effect in mice (Dai et al., 2014).

10. S. gordonii -M6-Sj-F1

a. Vaccine Ontology ID:

VO_0004668

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Immunization Route

Intramuscular injection (i.m.)

IV. References

1. Balen et al., 2007: Balen J, Zhao ZY, Williams GM, McManus DP, Raso G, Utzinger J, Zhou J, Li YS. Prevalence, intensity and associated morbidity of Schistosoma japonicum infection in the Dongting Lake region, China. Bulletin of the World Health Organization. 2007; 85(7); 519-526. [PubMed: 17768500].

2. Chen et al., 2016: Chen L, Chen Y, Zhang D, Hou M, Yang B, Zhang F, Zhang W, Luo X, Ji M, Wu G. Protection and immunological study on two tetraspanin-derived vaccine candidates against schistosomiasis japonicum. Parasite immunology. 2016; 38(10); 589-598. [PubMed: 27189226].

3. Da'dara et al., 2008: Da'dara AA, Li YS, Xiong T, Zhou J, Williams GM, McManus DP, Feng Z, Yu XL, Gray DJ, Harn DA. DNA-based vaccines protect against zoonotic schistosomiasis in water buffalo. Vaccine. 2008; 26(29-30); 3617-3625. [PubMed: 18524429].

4. Gan et al., 2004: Gan Y, Shi YE, Bu LY, Zhu XH, Ning CX, Zhu HG. Immune responses against Schistosoma japonicum after vaccinating mice with a multivalent DNA vaccine encoding integrated membrane protein Sj23 and cytokine interleukin-12. Chinese medical journal. 2004; 117(12); 1842-1846. [PubMed: 15603716].

5. Gao et al., 2017: Gao Y, Zhou X, Wang H, Liu R, Ye Q, Zhao Q, Ming Z, Dong H. Immunization with recombinant schistosome adenylate kinase 1 partially protects mice against Schistosoma japonicum infection. Parasitology research. 2017; 116(6); 1665-1674. [PubMed: 28455627].

6. Ke et al., 2017: Ke XD, Shen S, Song LJ, Yu CX, Kikuchi M, Hirayama K, Gao H, Wang J, Yin X, Yao Y, Liu Q, Zhou W. Characterization of Schistosoma japonicum CP1412 protein as a novel member of the ribonuclease T2 molecule family with immune regulatory function. Parasites & vectors. 2017; 10(1); 89. [PubMed: 28212670].

7. Wei et al., 2009: Wei F, Liu Q, Zhai Y, Fu Z, Liu W, Shang L, Men J, Gao S, Lian H, Jin H, Chen C, Lin J, Shi Y, Xia Z, Zhu XQ. IL-18 enhances protective effect in mice immunized with a Schistosoma japonicum FABP DNA vaccine. Acta tropica. 2009; 111(3); 284-288. [PubMed: 19467215].

8. Zhou et al., 2000: Zhou S, Liu S, Song G, Xu Y, Sun W. Protective immunity induced by the full-length cDNA encoding paramyosin of Chinese Schistosoma japonicum. Vaccine. 2000; 18(27); 3196-3204. [PubMed: 10856799].