VIOLIN: Vaccine Investigation and Online Information Network

Arthritis

Table of Contents

General Information
1. Introduction
Vaccine Related Pathogen Genes
1. COL2A1 (Protective antigen)
2. Collagen type II (Protective antigen)
3. Collagen type II (Protective antigen)
4. hsp65 (Protective antigen)
Vaccine Related Host Genes
Vaccine Information
References

I. General Information

1. Introduction

Arthritis is inflammation of one or more of your joints. The main symptoms of arthritis are joint pain and stiffness, which typically worsen with age. The two most common types of arthritis are osteoarthritis and rheumatoid arthritis. Osteoarthritis is usually caused by normal wear and tear, while rheumatoid arthritis is an autoimmune disorder. Other types of arthritis can be caused by uric acid crystals, infections or even an underlying disease, such as psoriasis or lupus (Mayo Clinic - Arthritis).

II. Vaccine Related Pathogen Genes

1. COL2A1

Gene Name : COL2A1
Sequence Strain (Species/Organism) : Gallus gallus
NCBI Nucleotide GI : 15546069
NCBI Protein GI : 15546070
Protein Accession : AAK98621.1
Other Database IDs : CDD:189968
CDD:279718
Taxonomy ID : 9031
Gene Strand (Orientation) : ?
Protein Name : alpha 1 type IIA collagen precursor
Protein pI : 8.66
Protein Weight : 132343.91
Protein Length : 1506
Protein Note : breed: San-Huang

DNA Sequence : Show Sequence

>gi|15546069|gb|AY046949.1| Gallus gallus alpha 1 type IIA collagen precursor (COL2A1) mRNA, complete cds, alternatively spliced
ACTGCTCGGCCCGACGGACGGTGAGAGGGCCTCCGCCGCCCGCCATGCACGGCCGCCGCCCGCCCCGCTC
CGCCGCTCTCCTCCTCCTCCTCCTCCTTCTCACGGCCGCCGCAGCCGCGCAGGACCGCGACCTCCGACAA
CCTGGCCCCAAGGGACAGAAGGGAGAACCCGGAGATATTAAAGATGTTGTAGGACCCCGAGGGCCTCCAG
GACCACAGGGCCCAGCAGGAGAGCAGGGACAGCGAGGGGACCGTGGCGAGAAGGGGGAGAAGGGTGCTCC
TGGCCCCCGTGGGAGGGATGGAGAACCCGGCACCCCTGGAAACCCAGGCCCCCCCGGTCCCCCCGGACCT
CCTGGCCCCCCCGGACTTGGTGGAAACTTTGCGGCGCAGATGGCGGGCGGCTTCGATGAGAAGGCGGGTG
GAGCGCAGATGGGTGTCATGCAGGGACCCATGGGCCCTATGGGACCCCGCGGCCCCCCTGGCCCCACTGG
CGCACCTGGTCCCCAGGGATTTCAAGGCAACCCCGGTGAGCCCGGCGAACCCGGCGCTGCTGGTCCGATG
GGTCCCCGGGGACCTCCGGGACCACCTGGGAAACCCGGTGACGATGGTGAGACAGGCAAACCCGGCAAAT
CTGGTGAACGTGGCCCCCCCGGCCCCCAGGGCGCTCGTGGCTTCCCTGGGACTCCTGGTCTCCCCGGAGT
GAAGGGCCACCGAGGCTACCCCGGTTTGGATGGTGCCAAAGGAGAGGCGGGGGCTCCTGGAGCCAAGGGT
GAATCTGGTTCACCGGGTGAGAACGGCTCCCCCGGCCCCATGGGACCCCGTGGGCTGCCCGGAGAGCGAG
GACGTCCCGGCCCCTCCGGCGCCGCCGGTGCTCGTGGCAATGACGGTCTCCCTGGCCCTGCTGGACCCCC
TGGACCCGTCGGCCCTGCCGGAGCCCCCGGCTTCCCCGGAGCCCCCGGTTCAAAGGGTGAAGCCGGCCCC
ACTGGTGCACGGGGTCCCGAGGGTGCCCAAGGACCCCGCGGCGAATCCGGCACCCCCGGCTCTCCCGGCC
CCGCTGGCGCACCCGGTAACCCAGGGACTGATGGCATCCCCGGTGCCAAGGGCTCGGCGGGTGCCCCGGG
CATTGCAGGCGCTCCAGGATTCCCCGGCCCACGCGGCCCCCCCGGACCCCAAGGTGCCACCGGACCACTG
GGACCCAAAGGACAGACGGGCGAACCCGGCATCGCAGGCTTCAAGGGCGAGCAAGGACCGAAGGGCGAGA
CGGGCCCCGCAGGACCCCAAGGTGCCCCCGGGCCGGCTGGTGAGGAAGGCAAGAGAGGAGCTCGTGGTGA
ACCTGGTGCCGCCGGCCCTGTGGGCCCCCCCGGAGAAAGGGGCGCTCCTGGCAACCGTGGATTCCCCGGG
CAGGACGGGCTGGCCGGACCCAAGGGTGCTCCAGGTGAACGCGGCCCCGCTGGTCTCGCCGGTCCCAAAG
GTGCCACCGGTGACCCCGGACGTCCCGGAGAGCCCGGGCTGCCCGGAGCGAGGGGTCTCACCGGCCGCCC
CGGCGATGCGGGACCTCAAGGCAAAGTCGGCCCAACTGGTGCTCCTGGCGAGGATGGCCGCCCCGGCCCC
CCCGGACCTCAGGGTGCTCGTGGGCAGCCTGGTGTGATGGGTTTCCCCGGTCCCAAAGGCGCTAATGGTG
AGCCTGGAAAAGCTGGAGAGAAAGGACTGCCCGGCGCCCCAGGGCTGCGGGGTCTGCCTGGCAAGGATGG
GGAGACGGGAGCTGCCGGCCCCCCTGGACCCGCTGGTCCTGTGGGTGAGAGAGGAGAGCAAGGAGCCCCC
GGTCCTTCCGGCTTCCAGGGACTGCCCGGACCACCAGGTCCCCCTGGGGAGAGCGGCAAACCCGGAGACC
AGGGTGTTCCTGGAGAAGCCGGTGCCCCCGGTCTTGTTGGTCCCAGAGGTGAACGTGGATTCCCCGGTGA
ACGCGGCTCTCCCGGTGCCCAAGGGCTGCAGGGTCCCCGTGGGCTCCCCGGAACGCCCGGCACTGACGGA
CCCAAGGGTGCAACCGGTCCAGCCGGCCCCAACGGTGCCCAGGGTCCCCCAGGGCTGCAGGGAATGCCCG
GTGAGAGAGGAGCAGCTGGCATCGCTGGCCTCAAGGGTGACCGGGGAGATGTTGGTGAGAAAGGACCTGA
GGGAGCTCCAGGCAAGGATGGCGCACGTGGTCTGACGGGTCCCATTGGTCCCCCTGGCCCTGCTGGCCCC
AACGGTGAGAAGGGTGAATCCGGCCCTCCTGGTCCATCTGGTGCTGCCGGTGCCCGTGGTGCCCCCGGTG
AGCGTGGCGAGCCCGGTGCCCCCGGTCCTGCTGGATTTGCTGGCCCCCCGGGCGCCGATGGACAACCCGG
TGCCAAAGGCGAGCAGGGAGAGCCCGGGCAGAAGGGTGACGCGGGCGCTCCTGGTCCCCAAGGTCCCTCC
GGCGCTCCTGGCCCCCAGGGCCCAACCGGTGTCACTGGTCCCAAAGGAGCTCGTGGGGCTCAGGGTCCCC
CTGGAGCCACGGGATTCCCCGGAGCTGCCGGCCGTGTGGGACCGCCCGGCCCTAATGGTAACCCAGGCCC
CCCCGGACCCCCTGGCTCTGCTGGCAAGGACGGCCCCAAGGGTGTTCGTGGCGACGCCGGCCCCCCCGGC
CGTGCAGGTGACCCCGGCCTCCAAGGCCCCGCCGGCCCCCCCGGCGAGAAGGGCGAACCCGGCGAGGACG
GCCCCGCGGGTCCCGACGGCCCCCCCGGCCCTCAAGGCTTGGCAGGACAGCGTGGTATTGTGGGTCTCCC
AGGACAGCGTGGTGAGAGAGGCTTCCCCGGACTGCCGGGGCCATCGGGAGAACCTGGAAAGCAAGGAGCG
CCTGGCTCTGCGGGTGACCGAGGTCCCCCCGGCCCCGTGGGGCCCCCTGGGCTGACGGGTCCTGCTGGAG
AACCCGGGCGCGAGGGCAACCCTGGTGCTGACGGTCTCCCAGGCAGGGATGGCGCAGCTGGCGTGAAGGG
TGATCGTGGTGAGACCGGCCCTGTGGGTGCCCCCGGTGCTCCTGGAGCCCCTGGCGCCCCCGGCCCTGTT
GGTCCCACTGGAAAACAAGGAGACAGAGGCGAGACGGGTGCACAAGGGCCCATGGGTCCCTCTGGTCCCG
CTGGAGCTCGAGGAATGCCGGGTCCCCAAGGACCTCGTGGTGACAAAGGTGAGACGGGAGAGGCTGGAGA
GAGAGGGCTGAAGGGCCACCGTGGCTTCACCGGTCTGCAGGGTCTGCCCGGACCACCCGGCCCGTCTGGA
GACCAAGGTGCTGCCGGTCCCGCTGGTCCCTCCGGTCCCAGAGGTCCCCCTGGTCCCGTCGGCCCCTCTG
GCAAAGATGGCTCTAACGGCATGCCCGGCCCCATCGGTCCTCCCGGTCCCCGTGGACGGAGTGGTGAACC
CGGCCCTGCGGGTCCTCCTGGAAACCCCGGTCCTCCCGGTCCTCCTGGCCCCCCCGGCACCGGCATCGAC
ATGTCTGCTTTTGCTGGACTGGGTCAGACGGAGAAGGGCCCCGACCCCATCCGCTACATGAGGGCAGACG
AGGCGGCCGGAGGGCTGCGGCAGCACGACGTGGAGGTGGATGCCACCCTCAAATCCCTCAACAATCAGAT
TGAGAGCATCCGCAGCCCCGAGGGCTCCAAGAAGAACCCTGCCAGGACCTGCCGCGACATCAAACTCTGC
CATCCCGAGTGGAAGAGCGGAGATTACTGGATTGACCCGAACCAGGGCTGCACCTTGGACGCCATCAAAG
TATTCTGCAACATGGAGACGGGCGAGACCTGCGTCTACCCGACCCCCAGCAGCATCCCCAGGAAGAACTG
GTGGACCAGCAAGACGAAAGACAAGAAGCACGTCTGGTTTGCAGAGACCATCAACGGCGGTTTCCACTTC
AGCTACGGCGATGAGAACCTGTCCCCCAACACCGCCAGCATCCAGATGACCTTCCTGCGCCTCCTGTCCA
CCGAGGGCTCCCAGAACGTCACCTACCACTGCAAGAACAGCATCGCCTACATGGACGAGGAGACGGGCAA
CCTGAAGAAAGCCATCCTCATCCAGGGATCCAACGACGTGGAGATCAGAGCCGAGGGCAACAGCAGGTTC
ACCTACAGCGTCTTGGAGGACGGCTGCACGAAACACACTGGCAAATGGGGCAAGACGGTGATCGAGTACC
GGTTGCAGAAGACCTCGCGCCTGTCCATTGTAGATACTGCACCTATGGACATTGGCGGAGCCGATCAGGA
GTTTGGCGTGGATATTGGCCCAGTCTGCTTCTTGTAAAAAGGGTTGTTGTTATTTGTGTGTTTGTTTGTT
GTTTGGTTGTTGTTTTTTGTTTCTTTTTTTTTTTTTTTTAGAAAAGAAAGGAATCCAGCCCAATCCCATA
AAAGCAAACCAGTCCCACCCCCAGGACCCGCACGTTCCCAGCACAACTTCTGCACTGAACGGATGGCACG
ACCCCGCGCCCCTTCGGGACCCTCCGGCGCCGTCACCGGGCAGACTGCGAAATACAACCACGGGCTTATA
TTTATTTATTGCCTTCCTGGAAGGCCTGGTTTCGTAGGGCGGGTGGAGGTGGGAATCAATCTGGCAGGTG
TGACGGCCCCCCTCCCCACAAAGGGATCTGGCAAACGCAGGTATCGCGAATCCCCTCCCCTCCCCGTGTA
TCACCAGCAGGAGTGCTAATGTATCATACAACAGAAATGGTGCTATTCTTGTAAAACAAGTCTGTATTTT
TTAACATCAGTTGATATAAAAACAACAAAAAAAAAAACTTTTGGTGGAAAGTAAAAAAAACAAAAAAAAA
AAAAAAA

Protein Sequence : Show Sequence

>AAK98621.1 alpha 1 type IIA collagen precursor [Gallus gallus]
MHGRRPPRSAALLLLLLLLTAAAAAQDRDLRQPGPKGQKGEPGDIKDVVGPRGPPGPQGPAGEQGQRGDR
GEKGEKGAPGPRGRDGEPGTPGNPGPPGPPGPPGPPGLGGNFAAQMAGGFDEKAGGAQMGVMQGPMGPMG
PRGPPGPTGAPGPQGFQGNPGEPGEPGAAGPMGPRGPPGPPGKPGDDGETGKPGKSGERGPPGPQGARGF
PGTPGLPGVKGHRGYPGLDGAKGEAGAPGAKGESGSPGENGSPGPMGPRGLPGERGRPGPSGAAGARGND
GLPGPAGPPGPVGPAGAPGFPGAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPAGAPGNPGTDGIPG
AKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIAGFKGEQGPKGETGPAGPQGAPGPAGE
EGKRGARGEPGAAGPVGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPAGLAGPKGATGDPGRPGEPGLP
GARGLTGRPGDAGPQGKVGPTGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPG
LRGLPGKDGETGAAGPPGPAGPVGERGEQGAPGPSGFQGLPGPPGPPGESGKPGDQGVPGEAGAPGLVGP
RGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGATGPAGPNGAQGPPGLQGMPGERGAAGIAGLKGDR
GDVGEKGPEGAPGKDGARGLTGPIGPPGPAGPNGEKGESGPPGPSGAAGARGAPGERGEPGAPGPAGFAG
PPGADGQPGAKGEQGEPGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGP
PGPNGNPGPPGPPGSAGKDGPKGVRGDAGPPGRAGDPGLQGPAGPPGEKGEPGEDGPAGPDGPPGPQGLA
GQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGSAGDRGPPGPVGPPGLTGPAGEPGREGNPGADGLPG
RDGAAGVKGDRGETGPVGAPGAPGAPGAPGPVGPTGKQGDRGETGAQGPMGPSGPAGARGMPGPQGPRGD
KGETGEAGERGLKGHRGFTGLQGLPGPPGPSGDQGAAGPAGPSGPRGPPGPVGPSGKDGSNGMPGPIGPP
GPRGRSGEPGPAGPPGNPGPPGPPGPPGTGIDMSAFAGLGQTEKGPDPIRYMRADEAAGGLRQHDVEVDA
TLKSLNNQIESIRSPEGSKKNPARTCRDIKLCHPEWKSGDYWIDPNQGCTLDAIKVFCNMETGETCVYPT
PSSIPRKNWWTSKTKDKKHVWFAETINGGFHFSYGDENLSPNTASIQMTFLRLLSTEGSQNVTYHCKNSI
AYMDEETGNLKKAILIQGSNDVEIRAEGNSRFTYSVLEDGCTKHTGKWGKTVIEYRLQKTSRLSIVDTAP
MDIGGADQEFGVDIGPVCFL

Molecule Role : Protective antigen

2. Collagen type II

Gene Name : Collagen type II
Sequence Strain (Species/Organism) : Gallus gallus
NCBI Gene ID : 395069
NCBI Protein GI : 45383309
Genbank Accession : AADN05000742
Protein Accession : NP_989757
Taxonomy ID : 9031
Chromosome No : 33
Gene Starting Position : 7292547
Gene Ending Position : 7311105
Gene Strand (Orientation) : +
Protein Name : transcript variant X1
Protein pI : 8.52
Protein Weight : 131719.18
Protein Length : 1420

DNA Sequence : Show Sequence

>NC_008465.4:7292547-7311105 Gallus gallus breed Red Jungle Fowl isolate RJF #256 chromosome 33, GRCg6a
ACGCGGCGTCGGGGCGGTGCGGCACTGCTCGGCCCGACGGACGGTGAGAGGGGCTCCGCCGCCCGCCATG
CACGGCCGCCGCCCGCCCCGCTCCGCCGCTCTCCTCCTCCTCCTCCTCCTTCTCACGGCCGCCGCAGCCG
CGCAGGACCGCGACCTCCGTGAGTACCACCCGCCGCCCCCCCCCAGGCCCCCCCCGCTCCACCCCGTCGG
GACTCGGAGCTGCGGGATCCCTGCGGTGCGGTGGGGGTGCGGGGTGGGGGGTTGGAAGGGGGGAAAGTGG
GGTGGAGGTGGAAGTTGTGTGTGTGGGGGGGGGGACAGCGTGGCGTGGAGGGGGGTGGGGGGGAAAGTGC
GAAGGAGAACGCGAGATGGGGGGGGCGGTGTGGGGTATGTGGGGAAAGTGATGGAGAGAGTGTGAGGATG
GGGGGGACGGTGGGGGGAAATGGGGTGAAGTGTGAGGATGGTGAGGGGGGCGGTGTGGGACAGTGAGGAC
GCGTGGGGTGGGGGGGACAGTGAGGGATACGTGGGGAAAGTTTGGGATGAGTGGGGAAAGTGATGGGGAC
AGCGTGGGGGTGGGGGGGACGGATGGGACGGTGCAAAGATAGTGGGGTGACAGCGTGGGGTATGTGAGGA
CTGCGTGAGGATGGTGGGGGGGGGACAGTGGGGGACAGGTGGGGAAGTGTGAGGATAGTGGAGGGAAGTG
GGGGGAACTGTGGGGTACATGGGGACAGTGTGGGATAAATGGGGACAGCGTGAGGGTGGTGGGGGGGGGG
ACACAGCAGGGAACAGTGTGGGACAGATGGGGAATGTGTGAGGACAAAGGGGGGACGGTGTGGGAACGGG
GGGGACACAGATTGAGACAAAGTGGGCTGATAGGGGGAAAGTGTGACAGTGGGGGTGGGGACAGCGTGGG
ACACTGCAGGCTGTGTTAGGACGGGAAGATTGTCTGGGATGGGGGGGGACAGCCTGGGAATGGGGTTTGG
GCAGCACTGGGACCATAGCGTACAGCTGAGACAGTGGGGTGCAGAGTGGGGACGGAGAGCTGCAGTGTGG
AGCAGTGGGGGACAGCCTGGGGACACAGGTCCAGCTGGGGATGGGATGTGGGGTCCCACTGGGACGTGGG
GTCCCATTGGGGACATGGGGTCCCATTGGGGACATGGGGTCCAGCCCTGGGGCCCCTCCTGGCCACCCCG
GGGGGCCTCTGGGGACCCTGGAGCACCTCGTGGAGATGCCAGGACACATCCCTGAGGCACGGGGACACCT
CCTGGGGGGGATTTTGGGGGTGGCTTCTGGCCATGCAGGGGTACCTCGTGGGGACACATCCTGGGGACCC
TGGGGTACCCCCTGAACACACGGGGATGCCTCTTGGGACCCTGGGGTGTAGCCTGGGGGGGGTCCCCAAG
GCAGAACATGGTGACTTTGGGCCACCTGCGGGGGATCCCAGTGAGGGACACTTCTTGGGGACCCCAAGGC
AACGCATGGGGACTTCAGGGCCCCTCCTGGGGTCCCTGGGACATCTTTTGGGGACACTGAGACACTTCTT
GGAGTCCCCAAGGAACAACCTGGAGACTTTGGGGCCCCTCTTGGGGTCCCCGGGACATCTTCTTGGGACC
TCGGGGGACCACCTGGGGATGCTGAGGCTCAGTTTGGGGACAGTGGGACACCTTTTGGAGACCTCGGGGA
CACCGCGTGGGGACCTGAGGCACAAGTGGGGACCCTGGGGCACCACCCGAGGACCCCCAGGCACACCCCG
GAGACCTCAGGACCCTTTTTGTGGACCTTTGGACACCTCCTGGGGACGAACCCTGCAGACCCTTCGGGCT
CCATGGGAGCACCGGGGATGCCCCCCCCCCCCCCCCCCCCCCAGGTTCAGTCCCACCAGAAAGCGCTGAT
TGTCCGCGAGGGTCCCCGTGTGGCACAGTGACACCGAATGCCATCGGGGTTGGGGCACTCCGGGAGAGGT
CTCCTCACTTCCCCCCCCCCCCGCCCCCCCGCTGCGCACCGCCTCGCTGCTTGGGGATCCTTCCTCCTCC
TCCTCCTCCTCCTCCTCCTCTGGATGGGATGGAGGCATCGCATCCACACCTCCCCCCCCCGCCCCCGCTG
CCGAATCCCCGCGTTTCGCTCCATCCCATGAATCGCTCCATTCATTGCGCACAGCGAAGGCAAACCGGGA
GGGGAAGGAGGGTGGGATCGGGGAGGGGGGGGGGTGGGGGGTTCCCCATGCCACCACGGTGCGGGGGTGA
TGCCGTCCCCCCCTCCCCACTCCTCCCCCCGTTCCCCACCCCATCGCCCCCGCAGCGGAGGCCGCCGGGG
GCTGCGTGCAGGACGGGCAGAGCTACAGCGATAAGGACGTGTGGAAGCCGGAGCCGTGCCGGATCTGCGT
GTGCGACACCGGGACCGTTCTGTGCGACGAGATCATCTGCGAGGAGCCGCAGGACTGCCCCAGCCCCGAA
ATCCCCTTCGGAGAGTGCTGCCCCGTCTGCCCCGCAGAGCTGCCCGCCGCCGCCCGTAATTTATTTATGA
CGCCCCCCCCCCCCCGTCCCCCCAAATCACTTTAATGACCCCCCCCCCCCTCCCCCCCTCCTCCCTCCAC
AGATAAATAAATTACCGTGCGCTTAATTGCCCCATGGATGGGGGGCGATCCATGGGGACGGCGGGGAAAA
GGCAACTTGGGACCCCCTCCCTAATAGGACCACCACCTCCCCCCCATAGGGACCCCCCCGGGGATCTCTC
TCCCCCAAACAGCCGCTTTTCAACATTTTTGGCCCCTTCTTGCCTTTTTTGGGGGCCCTTTGGGGCCTTT
TATTGCCCCTTTTGGTCTCTATTTGCTCCATTTTGCCTTTTTGGAGTCCCTTTTATTGCCCCTTTTTTCC
TCCATTTCCCCCTTTTTTGCCCTTATTTGCCCCCTTTTTGCCCTTTTTGACCCCTCTTCATCATTTATTA
CCCTTTTTTTTGCCACTCTTTACCCCATTCTTGGCACTCTTTGCCCCTTTGGGGTCTTTAATTCCCCCTT
TTCCCCCTTTTCCCTTTTTTGCCCAATTTTTGCCCCCATTTGGCCCTGTTTTGCCATTTTGGGCTCCTCG
TGGGACATTTATTGCCCCCTTTTCCCTTTCTTGCCCATTATTTGCCATTTTGGGCCCTTTATTCTCATTT
ATTCCTTCTTTTATCCTCTTTTTTTCCCTTTAATGCCCCCTTTTCTCCTCTGTTTTCCCCCTTTTTTTGC
CCTTATTGTCACTTTTTGGCCCTTTTGGGGCCCTCTTGGAGCTTTTCTTTCCCCTTATTTATCTTTTATG
CTCCATTTTGCCATTTTTTTGCACGTTTTTTGCCATTTTGGAGCCCCTCTGTTCCCCCTTTCCCCACTTA
TTGCCCCTTTTTTCTCTGTTTACCCAATTTTCTCCTATTTTGGCCATTTTCAGCCTTTTTTTGCCCCCCT
CCCCCCCTTTGTTCTCCTTTCCTCCCTTTTCCTCCTCTTCTCCCACTTTCTGGCCTTCCCCCCCTCCCCT
TTTTTGCTTTATTTTCCCTTTTATTGATGATTTTTTGCCCCGTTTTCACCTTCTTGGCCATTTTCTTCCC
TTTTGCTTCTCTTTTTACTGCCTTTTAACCCATTTCTCCCCCCTTCCCCCCTTTTTTTTTGCCTTAATTT
ACCTCTTTTTGGTCCCTTTTTGCCCCTTTTTTTTGGCCTTTTTTATCTCCTTTGTTTCCCCCCATTAATG
TTTGCCTTTTTTTCCCCATTTTCCCATTTTTGCTCCCCCCTTTTGCCCTTTTTTTGCCCTCTTTTCCCCT
CAGTTGTGACCATTTTCTCCCCATTTTTTTCATTCTCAGTGCCCCCCCCCCCATTTTGCACAACCAAACA
CACCCCATGAGGTTGGGGGGGTCACAGCCCCGGGTTGGATTTGGATTTGTTTTTCCTCCCCACAGTGATT
TGGCTGCCCCAAATCTCCCATTATTCACCCCAACCCCATTTCCACCCCCAGTGATGGGGAAGAGGCGCTG
ACCCCATTTTTCCCACATCAGCCCAACCCCCCCCCCCCCTCACCCCAATCCCCCCCCCCCAAACCCCAAA
GTTGCCTTTTTTTAACCCTTTCCTGCCCCCAGCCATGGAGGGGTGAAGGGTTGAGCATTTCCCCCAGCCC
AAAGCGCGTGTCCCCGCTTCCGCGTGCTTCGCTTCTGGGGGGGCCCTGAACCCCCCCCAGTGCCCCCAGT
GCACCCCAGTTTGACCAACCCCCCCCCCTCCTCGCTCTAATTTATCATTCTTTCTTTCCCTTCCCAACAG
GACAACCTGGCCCCAAGGTGAGGCTGTTCCCCCCTCCCACCTCATTTCCAGCATAATCCCAAATTCTGGG
GATTTTCAACCTTTTTCTTTGGGGGGGGGGGGGAGGGAGGGGGGGGACTGAATCCTTGGACATGTGTATG
GGGATTCGGGTTTGTCTCCTCCTGGGAGGGGTGTTTCACCTCCTGGGGGGGGTTGGTCGCTTCTTGGGGG
GGGGGGGGAATCAGAAAGTTGGGGGTGGGCACAAGAGGGGCACCCCCGCTCTCTCTCACCCCCATCTTTG
CTTTGCAGGGACAGAAGGGAGAACCCGGAGATATTAAAGATGTAAGTGATGGAGAGCAGGGGGGGTCCCC
AACATGGGATGGGGGGGTTGTTACCGTGGGGGTCTCCATGTGCCTTCCTCTCACCCTCCCATTTTCCCCC
CCCCCCCCCTTCCCCATGCAGGTTGTAGGACCCCGAGGGCCTCCAGGACCACAGGTGGGAGCGCGTTTCT
CCAGGCGCATTGCTGGAATATTTGTGGGGGGGGGGGGGGGGGGGTGGGAATTTTTTAGGGTGGGGGGGTG
GTGCTGGGGAGAGCTGACCCCCCCCTCACCTCCTTAGGGCCCAGCAGGAGAGCAGGGACAGCGAGGGGAC
CGTGGCGAGAAGGGGGAGAAGGTGAGCGCACCCCCAGAACCGGGGGGGGGGAGCACAAAGCCCATCCCCC
CCCCCCACCAACATGGAGCGTTCTGGGGTACAACAGTGCTGTGTCCCCCCCATAGGGTGCTCCTGGCCCC
CGTGGAAGGGATGGAGAACCCGGCACCCCTGGAAACCCAGGCCCCCCCGGTCCCCCCGGACCTCCTGGCC
CCCCCGGACTTGGTGGAGTGAGTATTGAGGGATGGAGGGGAGGGGGGGGGGGTGGGGCATTTTGGGGTGA
AACATGGCAGTTTTGGGGCTGAGCACTTCTCTGTTTGCAGAACTTTGCGGCGCAGATGGCGGGCGGCTTC
GATGAGAAGGCGGGTGGAGCGCAGATGGGTGTCATGCAGGGACCCATGGTAAAGCCTCCCCCTCCCCAAA
CCCCCCTCCCACCCCAAAACCAGGAGGCCCTCCAGAATTCACACCCCTCTCCCCCCCAGAAACTCAACCC
AAACCCACACCAAGGGGTCTCATCCCCTCAGCCCCATTGTGGGACATGGGGGACCCCACAGCACAGCCGC
CATTCTGGGGAGGGGTACCCTCTGTCTCTGCAGTCTGGGGTGGGGGTGTGATGGTGGTGAGAGCAACACC
CCCCCCCCCCCCATCTCCCCCATAAAACACCCCAAACTCACACCTGGGAGTCCCATAGGGGACCCTGTGC
CATCACCACTGTTCTCAGGGGGCACCCTCTGATTTTTTGGGGGGGAGGCGATGGTGTTGATGGCCGCCTC
GCTTCCCCCTCCCCCCCAAAAAAAAACCCCACAACCCAACAACACACACCTGGGGGTCTCATCCCTACTG
TGGGACATAGGGGGTGTCTCATCCCCCCAGCCCCACTGTGGGACAAAGAGGACCTGCAGCATCACCACCA
CTTGGGGGGGGGCACCCTCTGTTTTGGGTGGTGGGGGGGGGTGATGGTGATGACAGCCACCTCAACCCCT
CCCCCATGGTGACACTGTCCCTCATCTCTCTCCCTACAGGGCCCTATGGGACCCCGCGGCCCCCCTGGCC
CCACTGGCGCACCTGTAAGTACCCGCAGCCCCCCTGGGCTCCGGGGGGCTCCGGTTGGGTGTCCCACCGC
CCCCCCAATGCCACCCCCTGTGCTCTCTCCCCACTTTAGGGTCCCCAGGGATTTCAAGGCAACCCCGGTG
AGCCCGGCGAACCCGGCGCTGCTGTGAGTGTCCCCTCCTTGTCCCCACTCTGCCCCCCTTTGCGTCCCCC
CTCAACTTGGGGACGTGTCTCCTGCCCTCCTCCCCCTCCCCCCCCCACCTCACCGCTGTCTCTTTGTCCC
CTGTAGGGTCCGATGGGTCCCCGGGGACCTCCGGGACCACCTGGGAAACCCGGTGACGATGTGAGTCCCC
CCGTAACAGCGGTGCTGAGCACGAGCAGCTCGGTGCACTGATACCCCCCTTTCGAGCGCTGCTTCCTCTC
CATTAAAACCCCATTATTTCCCTGCAGGGTGAGACAGGCAAACCCGGCAAATCTGGTGAACGTGGCCCCC
CCGGCCCCCAGGTGAGGCAGCACTGGGCTGAACGTTGCCCCCATCGCTGCCCCCAACCCTTTTTCCCCTC
CCCACCTCAAGTTCACCCTAACCCTATTTCTCTCCACAGGGCGCTCGTGGCTTCCCTGGGACTCCTGGTC
TCCCCGGAGTGAAAGGCCACCGAGTGAGTGCCCCCCACCCACACCGTCCCCCCATCACTTATATCCCACC
GTGCCCCCTCATAACGTTGTTTCTGCCTCTCTCCCTTCACCCCCAACCAGGGCTACCCCGGTTTGGATGG
TGCCAAAGGAGAGGCGGGGGCTCCTGGAGCCAAGGTGAGTGGGGGGCGACCGCCTCCATGGCGTGGGGAT
GTGTACCCCAACCTGGGGCTCATCCTCTCTCGACCCTTTTTCCCATACAGGGTGAATCTGGTTCGCCGGG
TGAGAACGGCTCCCCCGGCCCCATGGTGAGTGCGGGGGGTGGGCATAGGGGAAACTGAGGCACAGAATAT
GAGGACGGACCCCATAACCTCTGCGCTGAGCCCAACCCAACACTGTGCCCCCTCCACCCCCGCAGGGACC
CCGTGGGCTGCCCGGAGAGCGAGGACGTCCCGGCCCCTCCGGCGCCGCCGTGAGTGTGACAGTGGCCCTA
TGGCACAGGGCTGCGTGTGGGGGGGGATCGGGGTGCTGTGACCCCCCCCATGACTGCCGTCTCCCCCCAG
GGTGCTCGTGGCAATGACGGTCTCCCTGGCCCTGCTGGACCCCCTGTAAGTATAGGGCTGCTTTGACCCC
ACACCTCAACTTTCTGCTGAACCCCGTAGGGGTTCTCCGGGAGCCCCCCCAGCCTGGGCAGCCCCCCCAC
ACTCTCCTGTTCCCCCAGGGACCCGTCGGCCCTGCCGGAGCCCCCGGCTTCCCCGGAGCCCCCGGTTCAA
AGGTAAGGGGTGAGCAGAGGGACGTGGGGAGGACTGGGGGGGGGGAAACTGAGGCGCAGTGCCCCGTCTC
ACCCCACTTCTCTCCCCCCAGGGTGAAGCCGGCCCCACTGGCGCACGGGGTCCCGAGGGTGCCCAAGGAC
CCCGCGGCGAATCCGGCACCCCCGGCTCTCCCGGCCCCGCTGGCGCACCCGTAAGTGTCCCCACTGTCAC
ATCCCTGGGATGTCCCATCCTCGTGGCATCCCCTTGGGCTCGGGAGCTGTGGGGATGGGGACACGGCGGG
GAGAGTGGGATGGGGGGGGGGATCTGAGCGGCAGAGGGGTTATCCTGTGCTTGGGGATGGGGAAACTGAG
GCACGGGGTGAGAGATGTCTCGCTGTTGTTCTTTTAGGGTAACCCAGGGACTGATGGCATCCCCGGTGCC
AAGGGCTCGGCGGTGAGTATGGACCAGGGTGGGAAGGGGGGAGGGGTCCCGGAACCTTTGGGGTGGAGCT
GGTGGGTCACGTGGGGGATGCTGGGAGTCATAAGGATGCTGGGGGGAGGGTTGGAGGGTGCTGAGGGGTA
CGGTGGAGATGTTGGGGGCGCTGGAGGTGCTGTTGGGGGGGGGGATGCTGGGGGGTGTGTAATGTGTGTG
GGATGCGGGGGTCACTGTGAGATGCTGGGGGGCAAGGGAGGATGATGAGGGATGTGGGGATGCTGGGGGG
TGTGTGGGAGTGCTGGGAGCCATGGAGTCATGGGGGGGGCCATGGGGCCGTGCCGTGCTCCCCCTGACGG
CTGCTGTGTGCCTGCAGGGTGCCCCGGGCATTGCAGGCGCTCCAGGATTCCCCGGCCCACGCGGCCCCCC
CGGACCCCAAGGTGCCACCGGACCACTGGGACCCAAAGGACAGACGGTAAGAGACCCCCTGAGACCCCCA
CGTGGGGACCCCGGGTATGGCCCCGCCCACTGCCAGGTGGCCCCGCCCATACTGGAGGTAGCCCCGCCCA
CTTTGCCCCAGGCCCCGCCCACCGTGCCGCAGGATGCGCCCCCTGCAGGACCCCAATGTGGGGCACTCTG
TGTCCCCTCCCCAAAGTGCCCCATGGTCACTCCCAGGAGACCCTCTTCTGGTCCCCAAAGGGGCCACTGG
GGATGGGATGGGTGATGAGGTTGGGGTGGGATGGGAATGGGATGAGATGAGATGGGAATGTGACGGTGAT
GGGGATGAGATGGGCACGTGGGTGGGTATAGGGACAGGAAAGAGGATGAGGAAAGGGCTGGGGACATGGA
TGGTGGTGGGGAGGAGGATAGGGTTGAGGATATGGGAAATGGGGGATGGGAAAGGGGATGGGGCTGAAGA
ATAAGAGGGAGATTAAGAAGGGGGCAGCTGTGGTTAGGGATTGAGGTGGGGATGGGGACACAGCTGGGAA
TAGATGGAGGCAGGAATGGGAGGGGTGTGCAGGGGAAAAGGGATGGAGGATACAGGTGGGGATGGAGTGG
GAATAGAGATGGGAGTGAGACTGTAGGGCAGGGAAAGGGATGGGGGACAGTGACGGTGTGGGGACAGAGA
TGGAATGGGGACAGGGATTTGATGGGGACAAGGATGCAGATAGGGGTGAAGGTAGGGGTAGGGGCAGGAA
AAATGCTGGAGAGATGGGGACGGGACGGAATTGGGATAGAAATGGGGTGGGGTGGGATGGGAATGAGGCC
GGGAATGGGAATGGGAATAGGGCTGGGGTTGGGATGGGGACCGCGATGGGGGCACTGAGAGCCCATTGCC
CACCGCCCTCTCTTCCCCCAGGGCGAACCCGGCATCGCAGGCTTCAAGGGCGAGCAAGGACCGAAGGGCG
AGACGGTGAGTTCTGGGGGGGCCCATCCAGCCCCCCCCCCATCCGGGGAGGACCCCGCTGCGGGGCTGCG
TCTCTAACGAACCTCTTCCCCTAACGAGGCCCCTCGAGGGGCAGCATCCCCCCTCCTGCAGCGGGGGGTG
GAGGGGGAACAATAGGCTCTGTGTTAGGGCCGCGCTGTGCCCCCCCCTCGGCCCCGGGGCCGGGGACATT
CCCCCATCGCTTCATTAACCAACCTCAATGCGTGGGGCCGCTTCCCACTGCGGGGCCGCTGAGCCCCGGC
GCCGTCGTGACATGCAGCACCGTGTGGACCCCCGGAGATGGGGCTTTTGGTTGGGGGGGGGGGGCTGCGA
ACTGAACCCCAACCCCCCACCCGTCCCGGTTTGCTGCTCTGACCCCTTTTCCTCCTCTTCCTCAGGGCCC
CGCAGGACCCCAAGGTGCCCCCGGGCCGGCTGGCGAGGAAGGCAAGAGAGGAGCTCGTGGTGAACCTGGT
GCCGCCGGCCCTGTGGGCCCCCCCGGAGAAAGGGTGTGTGCAGAGCGCTGCCCCCCACCCCGAAAAAGAC
AAAAACCCCACAGCCAGCCCTGACTTTGCCCCTCTTCTTCTTCTTCCATAGGGCGCTCCTGGCAACCGTG
GATTCCCCGGGCAGGACGGGCTGGCCGGACCCAAGGTGGGACTTCGGGGTTATGGGGGGGGGTCCTATAG
GGAGTGCCGACTCCCTGTGCCCCCCTCCAACCTGTGCCCCCCTTCCCCCCACACAGGGTGCTCCAGGTGA
ACGCGGCCCCGCTGGTCTTGCCGGTCCCAAAGGTGCCACCGGTGACCCCGGACGTCCCGGAGAGCCCGGG
CTGCCCGGAGCGAGGGTGAGAGCTGGGGGGGAGTCCCCATGGGGGGGTGGGGGTGCTGGGGGGGCTCAGG
TGGGGCAGAGGGATGTGGGGTGGTGGTGGTGGGGGCTGCTGGGGTCAGCAAGGAGGGGTTTGGGGAGCAG
TTCAGGGGGTTTTGGGGTTGGTTAAGGAGCATTTTGGGTTTGTTAAGGAGCATTTGGGGTTGGTTAGGGG
ACGTTTGAGGTTTGTTACAGAACATTTGGGGTTGGTTAGGGAGCTTTGGGGGGTTGATGCCTTTGGGGTT
GCTTCCTTTGGTTTCAGCCACGAAGCGCTTTGCCATGAAGGTGCTCTTTGGGGTTGGTTCAGGTGCTGCT
GGGCTCTGATCAGGATGCACTTTGGCATCAGCTGAGATGCACGTAGGGGTGGTTACGGTGCAGGTGGGGC
TCATTGTGGTGCATTAGGGGTGGGGGTGCTCCTTGGTGCTCATTAACGTTGTCTGTGGGGTGAGTTAGGA
TGCATTTGGGATGAGTTAAGTTGCTTTTGGAGTTACCAAAGGTGCTCCCTGGGGTTACTAAAGATGCTCT
TTAAGGGCACGCAGCCCCCCGTGCCCTCACCCGGCCCATTTCAGCCCTGGAGCTGGGCAGGGCAGGGGTT
CCCATTGTATCCCCCTCCCAACTGGGGGCCACCCGCTGCAGCCCCCACTGCTTCCTTCCCCTGCAGGGTC
TCACCGGCCGCCCTGGCGATGCGGGACCTCAAGGCAAAGTCGGCCCAACTGTAAGTGATGCTCTATGGGG
TGGGGGAAGGAGGGCTGGGGGTGGCTGTTGTGACCCACGCCGATGTTCCCCTCCCCATGTAGGGTGCTCC
TGGCGAGGATGGCCGCCCCGGCCCCCCCGGCCCTCAGGGTGCTCGTGGGCAGCCCGGTGTGATGGGTTTC
CCCGGTCCCAAAGGCGCTAATGTGAGTATTGACCATTTTCTGCCCCAAAATGGAGCTGTGAAGGTCAGCG
CAGCTGTGAGCCCATCCCGTCTCCTCTCTGTAGGGTGAGCCTGGAAAAGCTGGAGAGAAAGGACTGCCCG
GCGCTCCAGGGCTGCGGGTGAGCTCCTTCTGCCACTCTGACCCCATAAAATCACCTTGTGGGGTTTCCCC
TGCACTGTGCCCTGTATGTGGATGGGGAAACTGAGGCACGGGGCTGAGTGCCGGGGATTTCTCCTCTGCA
GGGTCTGCCTGGCAAGGATGGGGAGACGGGAGCTGCCGGCCCCCCTGGACCCGCTGTGAGTGTCCCTTCT
TTTCCTCTTGGGGACAGATGTTTGAAATGTGGGGCTGGGGGGTGTAGCCCCCAGATCTGGGTGGAAGACC
AGCCCAGGAGTGCTGGAGATACGGGGTGGGCAAAAGCCCCGGTGTTGAAGTGTGGGGATTTGGTGTCCCA
GCCGCCCCATCCCGGTGTGATTTGGATTTTGCCCAAAACCTTGGGGTGGGTGAGGGGCTGCAAAGGGGCG
AATCCAGGAGGGAGGAGGGATCTCAGAGCTGTCACTGCTGTCCTCCCTTTAGGGTCCTGTGGGTGAGAGA
GGAGAGCAAGGAGCCCCCGGTCCTTCCGGCTTCCAGGTGAGTGGGGACACACAAGGAGGGTTGGGGTGAT
GCAGGTGGGCACCCCCCCCTAACACCACTCCTTGTCCCCAAACAGGGACTGCCCGGACCACCAGGTCCCC
CTGGGGAGAGCGGCAAACCCGGAGACCAGGTGAGGCCATTCATCCCTAGGCTGCAAGTGGGGGCTTAGCC
TGTGCTGACCCCCCCCCCCCCCCCCCCCCAGTGCTGACCCCCCCAGTGCTGACCCCTGGCTCTTCCCTCC
TCTCTGCAGGGTGTTCCTGGAGAAGCCGGTGCCCCCGGTCTTGTTGGTCCCAGAGTGAGTATTGCTCATC
CCCCCCCCGCTCCTCCTGTCCCACTGAAGGGTTTGGGGGCTCAGGGGGGAGCAGAATCCAACCACCCCCA
CCAAAGCCATCGGACCCCCACAGTTCCAGCAGCTCAGTGTCCCCCCTCTCCTCCCAGTTCACCCCAATCC
CACCTGGGATGGATGCGCTGCCCTCTGTCCTGAGGACAGTGCCATTGTGTTCCTCACTATGGCCTCGGGG
GGCTTCTCTCTGACCGTCTCCCCCTCTCCCCCCCATAGGGTGAACGTGGATTCCCCGGTGAACGCGGCTC
TCCCGGTGCCCAAGGGCTGCAGGGTCCCCGTGGGCTCCCCGGAACGCCCGGCACTGACGGACCCAAGGTG
GGTGACAGAGGATGGGTGGCACAGTGCCACCACCATGGAGCCGTGATGCCATCGCGGCCCCAATGACCTC
ATCACTGTTCTGCTTTTAGGGTGCAACCGGTCCAGCCGGCCCCAACGGTGCCCAGGGTCCCCCAGGGCTG
CAGGGAATGCCCGGTGAGAGAGGAGCAGCTGGCATCGCTGGCCCCAAGGGTGACCGGGTAAGGTGATGCG
TCCCATAAGTGTCCTGAGTGACATCGGGTCTCAGCTCGGCTCACACTCATCCCATCCCACAGGGAGATGT
TGGTGAGAAAGGACCTGAGGGAGCTCCAGGCAAGGATGGCGCACGTGTAAGTGGGGCACGGCCATGGGGT
GGGCTGGCAAAGGATGCTCACAGAGACCACATCCTCATCTCTCTCTCTCTCCCATAGGGTCTGACGGGTC
CCATTGGTCCCCCTGGCCCTGCTGGCCCCAACGGTGAGAAGGTGAGAGCAGCATCACAGCACCCCACATT
ACGCCCCATGGGATGACCCCAGTGCCTCCACCTCTCCATCCTTTCTTTTCCAGGGTGAATCCGGCCCTCC
TGGTCCATCTGGTGCTGCCGGTGCCCGTGGTGCCCCCGTAAGCACAATGTCTGCAGCCCCTGGGTGCCCC
TAACCTTCACCCTAAACCCCCATCAACCCCTTTATCAACCTCCCCCATCTCTTCCCATTAGGGTGAGCGT
GGCGAGCCCGGTGCCCCCGGTCCTGCTGGATTTGCTGGCCCCCCGGTGAGTGTTTCACCCCGAAGCCCCC
ATCGCACACCCACGTCTTCACCCCACATCCTCACCCCACTCATGGTGGCTGCTGTTCCCATCAGGGCGCC
GATGGACAACCCGGTGCCAAAGGCGAGCAGGGAGAGCCCGGGCAGAAGGGTGACGCGGGCGCTCCTGGTC
CCCAAGGTCCCTCCGGCGCTCCTGGCCCCCAGGTACAACCCCAAATGGGGCAAACCCCCAAATTTGGGAC
GTCACGGCCCCAATGCAGGCACACTGCAGCTCCCGTTCGGATTTGTAACCTGTTTTTCTCTCCTTCCTAG
GGTCCAACCGGTGTCACTGGTCCCAAAGGAGCTCGTGGGGCTCAGGGTCCCCCTGTGAGTACCGGGGGGT
GGGCTGCAGGGTGGGGAAGGAACGGCCGTGGGGCTGAGCTGTGTCTGAGCCGTTTCTCCTCTCCCTCTCT
CCTCTGACTCTGTGATTCCCTCCCCAGGGAGCCACGGGATTCCCCGGAGCTGCCGGCCGTGTGGGACCGC
CCGGCCCTAATGTGAGTCTGGGGGCGTTCTGGGATTGCCCCCACCTGGGGTTTGGGTGCTGCTTCTCCGT
GCTGCGTGTTGGAGGGGGCACTGTTTCCCTGCACAGACACGTGGGGTTTTCCTCCTTGGCTCTCTGATGT
TGGCTTTTGGGGCCATTCCAGTGGTAGAGAAGGACTTTTCTAAGGGCAAGAGCTCCCCAAGAAGCAGCAG
TGGGATGCGGGTGATAAAGATGGAATGGCTGCCTCTGGTTTGCACCAGCGCTGCTTTCCTTCCCTTTAGG
GTAACCCAGGCCCCCCCGGACCCCCTGGCTCTGCTGGCAAAGACGGCCCCAAGGGTGTTCGTGGCGACGC
CGGCCCCCCCGGCCGTGCAGGTGACCCCGGCCTCCAAGGCCCCGCCGGCCCCCCCGGCGAGAAGGGCGAA
CCCGGCGAGGACGGCCCCGCGGTGAGGATTCTGGGGGTCTCCTCCCTCCGTGCACCCCCTGGCTGCGTGG
TGCCGTTGTTCTTAGTCTGATTTCCCCCTCTGCCGCCCTGCAGGGTCCCGACGGCCCCCCCGGCCCTCAA
GGCTTGGCAGGACAGCGTGGTATTGTGGGTCTCCCAGGACAGCGTGGTGAGAGAGGCTTCCCCGGACTGC
CGGGGCCATCGGTGAGTGGGTCGCTCTCATTTGGGTGCACTGAATCCTATGGGGTGCAGAGATGTGGGGG
CCGCGATGCTCTGGAGCCCATCTCAGGGGTCGCCAGCCCTTTGGTGCAGCCCGGGGACACCGTTTGCAGG
TGGGTTGGGGTTTTGCAGAGCTCCTTTTTCCCCACCAGGAGCCGCTGGTGCAAGGCTTAAAGCCGGGGCA
GGAAAACCATCGGTGGTTATTTGTTGCAGAGGGGTCTGGGAGCCATAAAAAACGGGGAAGGGGCAGCGCT
GGGGTCTCTCCCACTCATGCACTTCTTTCCCCTCTTTCAGGGAGAACCTGGAAAGCAAGGAGCGCCTGGC
TCTGCGGGTGACCGAGGTCCCCCCGGCCCCGTGGGGCCCCCTGGGCTGACAGGTCCTGCTGGAGAACCCG
GGCGCGAGGTAAGCAAAACCCCACAGCATCACAGCGGCACCGGGCATCACCAACCCCATGGCACAGCTCA
GCTCCCAGAGCTCCCCGGTGTCTTTTTCTCCAGCACTGAAAGGAGACTTTGCACAAATCCTGCTCCACCC
GGGTTGTAACATCCCCTTTTCCTCCTAGGGCAACCCTGGTGCTGACGGTCCCCCAGGCAGGGATGGCGCA
GCTGGCGTGAAGGTGAGCTTGCCATGCGCTCCCCATTGGCACTCGCCATCCCCGTGCCAAAAGCTGTGGG
GTTTTGCACAGATCTGACCTCTCTGTTGTCTGCTCGCAGGGTGATCGTGGTGAGACCGGCCCTGTGGGTG
CCCCCGGTGCTCCTGGAGCCCCTGGCGCCCCCGGCCCTGTTGGTCCCACTGGAAAACAAGGAGACAGAGG
CGAGACGGTGAGTGCTGGCACAAGGGTTTAGGGTTTAGGGTCTCCTTATGGCTGAAAATGTGCAGGGGTT
CCCCTCGAGGTTTGTTCCTTGCACCAGTGCTGAGAGCATTTAAAGATGCTGTGAGGCACCAATAGCTGCT
GATTGTCACTGTTGCCCGGATCTGGGGTGCGGAGCATGGGGCTGGCTCAGACACCCCCGAAATCCCAAAT
TCATGGCTTCGAGGTGGTGCTTCTGGTCGCTGGCACCTTCTGATGTCCTTTTTTTCTCCCTGCAGGGTGC
ACAAGGGCCCATGGGTCCCTCTGGTCCCGCTGGAGCTCGAGGAATGCCGGTGAGTGGTGCTGAGTGCATC
GGCACATCCCACGTACAGAGCGTGGGGTCCTCCGTGCCAGGAGGGGGTCTGCCACCCTGAGCCCGACACA
GCCCTGTCCCCACTTTAGGGTCCCCAAGGACCTCGTGGTGACAAAGGTGAGACGGGAGAGGCTGGAGAGA
GAGGGCTGAAGGGCCACCGTGGCTTCACCGGTCTGCAGGGTCTGCCCGGACCACCCGTAAGTTGGTTTGG
GGAGCACTGAGCCCCCCCCCCCCGTACGATGTGGCTCCTTTGGGGTCTCTGTGGCCACCGAGGCTCTGTC
TGGCCCAAAGTGCTGACCGCAGAGCTGTGACCACCCCGGCTTCCTCCTCAGGGCCCGTCTGGAGACCAAG
GTGCTGCCGGTCCCGCTGGTCCCTCCGGTCCCAGAGTAAGTCCTGACAGTGGTGTTTGGGGTGGTGGAAG
GGGAAGGAGCAGCAGTGGCCTCCCTGGGCACCTGCAGCCTCTGTTCGCTCCTGTCTGCTCATCAGCACCA
TCGCCTTCCCTGCCCTGAGGCCCCGCAATGCCTTCACCTCCCCGTTTTGGGGCTCTCTCCTAGGGTCCCC
CTGGTCCCGTCGGCCCCTCTGGCAAAGATGGCTCTAACGGCATGCCCGGCCCCATCGGTCCTCCCGGTCC
CCGTGGACGGAGTGGTGAACCCGGCCCTGCGGTGAGTCCTGGTGAGGGGAGGCAGGGAATGGGGTCCAGC
TCGCAGAGCAGCCCATCAGCATCACTTCTTTCTCCCATAGGGTCCTCCTGGAAACCCCGGTCCTCCCGGT
CCTCCTGGCCCCCCCGGCACCGGCATCGACATGTCTGCTTTTGCTGGACTGGGTCAGACGGAGAAGGGCC
CCGACCCCATCCGCTACATGAGGGCAGACGAGGCGGCCGGAGGGCTGCGGCAGCACGACGTGGAGGTGGA
TGCCACCCTCAAATCCCTCAACAATCAGATTGAGAGCATCCGCAGCCCCGAGGGCTCCAAGAAGAACCCT
GCCAGGACCTGCCGCGACATCAAACTCTGCCATCCCGAGTGGAAGAGCGGTAAGAGCTCCGCGTGCCTCT
CCCGTCCTCCCCTCTTCCCCACAGGAGAGCATCCCCAGCATCCTCGCACCGACCTGCGGTCAGGTTGGAT
GTTAGGAAAGATTCCTTGTCCAAAAGAGCTCTGGGCGCTGGGCTGGGCTGCCCGGGGAGGTGGGGCAGTC
GCTGTCCCCATAGGTGTTGGGGAACTGTGGAGATGTGGCACTTGGGAGCGTGGCTTAGTGGGGATGAGGC
AGCAGTTGGACCAATCTTCGAGGTCTTCTCCAGTCTTAATGGCTCTGTGCTTCTGTCGGTGTGCATGGTG
GTGATGGGTGGCCATTTAGACTTGGCGATCTTTGAGGTCTTTTCCGATCTTAACGACTCCTAGACCTCCC
CAACCCCATGAACGCTGTTTGTCCTCCCCCCTGCAGGAGATTACTGGATTGACCCGAACCAGGGCTGCAC
CTTGGACGCCATCAAAGTATTCTGCAACATGGAGACGGGCGAGACCTGCGTCTACCCGACCCCCAGCAGC
ATCCCCAGGAAGAACTGGTGGACCAGCAAGACGAAAGACAAGAAGCACGTCTGGTTTGCAGAGACCATCA
ACGGCGGTTTCCACGTGGGTGTCCCCCGGGTGTCCTTGGAAGGATCGATCCCACCTGGGATGTCCTTCTT
GCGGTCATGTGGATGGGTTTTAATGAAGTTATAGAGGGTGATTCTGAAGGTGTAGGTTTGGGTCAGTTCA
GCTCCACAAATCAAAGGGAAAGGATGGGATGGAGCAACTGAGCTCCCTCGGTTTGTTTGGCCCAGAAAAG
GTGAGGATGAGGGGAGGCCTCACGGCCCTACAGCCCCTTACGGCCCTACAGCAGCGTTAGGAAAAAAGTT
CTGCCCCGGAGCTGTGTTGGGCACAGAACAGCCCTGTGATGCCGGAGCTCGGGGAGCATTGGGACAACGC
TCTCAGACATTGGGTTTGGGTCAGGTCCTGGGTAACGTGATGTGCAGGGGGCAACCAGCCCATGGGTGGG
CTTTAAGGACCCTTCCAAGCCAACCATTCCATGGTTCTGTGATCTGTAAGGACCTTTCCAATCCAAACCA
CTCTGATTTTTTTCTCAGCCATTTGGGAACCTGAAGTACGGAAGTCCTCCCAAAAAGCTCCTGAGAGTAA
GGTGGTCATAATGCCCGCAGGCTTTAACTCCTCACCTCTTCCCTCCAGTTCAGCTACGGCGATGAGAACC
TGTCCCCCAACACCGCCAGCATCCAGATGACCTTCCTGCGCCTCCTGTCCACCGAGGGCTCCCAGAACGT
CACCTACCACTGCAAGAACAGCATCGCCTACATGGACGAGGAGACGGGCAACCTGAAGAAAGCCATCCTC
ATCCAGGGATCCAACGACGTGGAGATCAGAGCCGAGGGCAACAGCAGGTTCACCTACAGCGTCTTGGAGG
ACGGCTGCACGGTAGGTTGCTGGGCGCCTGCAAAGGAAAGGTGCAGATGGGGAGGGGGATGCTGAGGCTG
CGGGGATGAGGCCGGAGCAGCTGACAGCATCCCTGCCCTCCTTCCCTCCCCAGAAACACACTGGCAAATG
GGGCAAGACGGTGATCGAGTACCGGTCGCAGAAGACCTCGCGCCTGCCCATTGTAGATATTGCACCTATG
GACATTGGCGGAGCCGATCAGGAGTTTGGCGTGGATATTGGCCCAGTCTGCTTCTTGTAAAAAGGGTTGT
TGTTATTTGTGTGTTTGTTTGTTGTTTGGTTGTTGTTTTTTGTTTCTTTTTTTTTTTTTTTTAAAAAAAA
GAAAGGAATCCAGCCCAATCCCATAAAAGCAAACCAGTCCCACCCCCAGGACCCGCACGTTCCCAGCACA
ACTTCTGCACTGAACGGATGGCACGACCCCGCGCCCCTTCGGGACCCTCCGGCGCCGTCACCGGGCAGAC
TGCGAAATACAACCACGGGCTTATATTTATTTATTGCCTTCCTGGAAGGCCTGGTTTCGTAGGGCGGGTG
GAGGTGGGAATCAATCTGGCAGGTGTGACGGCCCCCCTCCCCACAAAGGGATCTGGCAAACGCAGGTATC
GCGAATCCCCTCCCCTCCCCGTGTATCACCAGCAGGAGTGCTAATGTATCATACAACAGAAATGGTGCTA
TTCTTGTAAAACAAGTCTGTATTTTTTAACATCAGTTGATATAAAAACAACAAAAAAAAAAACTTTTGGT
GGAAAGTAA

Protein Sequence : Show Sequence

>NP_989757.1 collagen alpha-1(II) chain precursor [Gallus gallus]
MHGRRPPRSAALLLLLLLLTAAAAAQDRDLRQPGPKGQKGEPGDIKDVVGPRGPPGPQGPAGEQGQRGDR
GEKGEKGAPGPRGRDGEPGTPGNPGPPGPPGPPGPPGLGGNFAAQMAGGFDEKAGGAQMGVMQGPMGPMG
PRGPPGPTGAPGPQGFQGNPGEPGEPGAAGPMGPRGPPGPPGKPGDDGETGKPGKSGERGPPGPQGARGF
PGTPGLPGVKGHRGYPGLDGAKGEAGAPGAKGESGSPGENGSPGPMGPRGLPGERGRPGPSGAAGARGND
GLPGPAGPPGPVGPAGAPGFPGAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPAGAPGNPGTDGIPG
AKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIAGFKGEQGPKGETGPAGPQGAPGPAGE
EGKRGARGEPGAAGPVGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPAGLAGPKGATGDPGRPGEPGLP
GARGLTGRPGDAGPQGKVGPTGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPG
LRGLPGKDGETGAAGPPGPAGPVGERGEQGAPGPSGFQGLPGPPGPPGESGKPGDQGVPGEAGAPGLVGP
RGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGATGPAGPNGAQGPPGLQGMPGERGAAGIAGLKGDR
GDVGEKGPEGAPGKDGARGLTGPIGPPGPAGPNGEKGESGPPGPSGAAGARGAPGERGEPGAPGPAGFAG
PPGADGQPGAKGEQGEPGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGP
PGPNGNPGPPGPPGSAGKDGPKGVRGDAGPPGRAGDPGLQGPAGPPGEKGEPGEDGPAGPDGPPGPQGLA
GQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGSAGDRGPPGPVGPPGLTGPAGEPGREGNPGADGLPG
RDGAAGVKGDRGETGPVGAPGAPGAPGAPGPVGPTGKQGDRGETGAQGPMGPSGPAGARGMPGPQGPRGD
KGETGEAGERGLKGHRGFTGLQGLPGPPGPSGDQGAAGPAGPSGPRGPPGPVGPSGKDGSNGMPGPIGPP
GPRGRSGEPGPAGPPGNPGPPGPPGPPGTGIDMSAFAGLGQTEKGPDPIRYMRADEAAGGLRQHDVEVDA
TLKSLNNQIESIRSPEGSKKNPARTCRDIKLCHPEWKSGDYWIDPNQGCTLDAIKVFCNMETGETCVYPT
PSSIPRKNWWTSKTKDKKHVWFAETINGGFHFSYGDENLSPNTASIQMTFLRLLSTEGSQNVTYHCKNSI
AYMDEETGNLKKAILIQGSNDVEIRAEGNSRFTYSVLEDGCTKHTGKWGKTVIEYRLQKTSRLSIVDTAP
MDIGGADQEFGVDIGPVCFL

Molecule Role : Protective antigen
Related Vaccine(s): Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen

3. Collagen type II

Gene Name : Collagen type II
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 12824
NCBI Protein GI : 169658375
Genbank Accession : AC113444
Protein Accession : NP_001106987
Taxonomy ID : 10090
Chromosome No : 15
Gene Starting Position : 97975601
Gene Ending Position : 98004723
Gene Strand (Orientation) : -
Protein Name : transcript variant 2
Protein pI : 8.58
Protein Weight : 131740.08
Protein Length : 1419
Protein Note : Also known as Dmm; Lpk; Col2; Del1; Col2a; Col2a-1; M100856; Rgsc856

DNA Sequence : Show Sequence

>NC_000081.6:97975601-98004723 Mus musculus strain C57BL/6J chromosome 15, GRCm38.p4 C57BL/6J
ATCCTTTCCAATGATTTTTTTTTTTTTGGTTTTTAATATCAATTGATGTTTTAAAAAATACAGAGGTGTT
TGACACAGAATAGCACCATTGTGTAGGACACGCAGTCCCTGCGCCCAGCATCTGTAGGGGTCTTCTGGCT
GGGCTGGGAACAGTCACTCGGGGGGCGTCTGACTCACACCAGATAGTTCCTGTCTCCGCCTGGACCCAGA
GGTCTTACAGGAAGACAATAAATAAATAGAACACCGAGATTTTATTTTGCAGTCTGCCCAGTTCAGGTCT
CTTAGAAAGGGGAGCACAGTCCGGGCTGTGAGGGGAGGACGGTTGGGTATCATCAGGTCAGGTCAGCCAT
TCAGTGCAGATCCTGGAGTGACTGCGGTTGGAAAGTGTTTGGGTCCTTTGGGTTCGCAATGGATTGTGTT
GTTTCAGGGTTCGGGGGTTTTACAAGAAGCAGACAGGCCCTATGTCCACACCAAATTCCTGTTCAGCCCC
TCCAATGTCCATGGGTGCGATGTCAATAATGGGAAGGCGGGAGGTCTTCTGTGATCGGTACTCGATGACG
GTCTTGCCCCACTTACCAGTGTGTTTCTAGGCAGAAAAGGGGAGGTGTCATTTGGGCGATGCCTTACAGC
AAGGACTGGCTACTTAGCACCCCCAAACTAAGCAGCCACAAGGTAGGGACGACACTGGGTGGCTTGCTAG
AACTTCTCTCCATTTGCCCAAGCCCTCTCCTCTTCAATTGTCCCCTTGATGGTAGAGCCCTCCTCTACTT
GTCTTCCTCCTTCCCACAGAGGGCTTCCTGCCTGGGATAGCCTCTCCTCTCCTATGCTAGTTTCTACGAA
TACTTCTATAAGAGTTAGTGAGCTTGCCAGCATCTTTATTGCCTCTGAATGAGGAAGTGGACAAGCCAGT
ATGGTATACGCTCCTCCCCCATCCCTGCCTCTCAGCTTGTCTCCCACATGCCCCCACGACTCAAGCCTCT
AATGAGTCCCTTCACTACAGGAAAGTGACATTTGTCACAGGCTGACCTCAGCCCCACTCACCGTGCAGCC
ATCCTTCAGGGCAGTGTATGTGAACCTGCTGTTGCCCTCGGCCCTCATCTCTACATCATTGGAGCCCTGG
ATGAGCAAGGCCTTCTTGAGGTTGCCAGCCGCTTCGTCCAGGTAGGCGATGCTGTTCTTACAGTGGTAGG
TGATGTTCTGGGAGCCCTCAGTGGACAGTAGACGGAGGAAAGTCATCTGGACGTTAGCGGTGTTGGGAGC
CAGGTTGCCATCGCCATAGCTGAACTGTGGGGCAGAGGGGCGAGGGTTGTGAGACCAGGGGCCTAGCATC
TGCTGGGATCAGAGGCTAACAGTACATTCATCTGATAGGGGCTCAGAGCATCAGATGGGCCCTGGGGCAG
GTGTGGAGCAAGCTCAGAGGACATCTTCTCTCGCTCTAATGGGGAAAATACTTTCTCCTCTTTCTTTCTT
CTAAGGCCCAAAGGGACACCGGCGGAGGATGCCAAGAAGGAGCTAAAGAATCGGAGGAGAGAGAAGAGCA
CTCAGGAGGGACCAGCTGAGCCTAGACCACCGGGCACTCACGTGGAAGCCACCGTTCATGGTCTCTCCAA
ACCAGATGTGTTTCTTTTCCTTGCTCTTGCTGCTCCACCAGTTTTTCCGAGGGACAGTCGCTGGGTTGGG
GTAGACGCAAGTCTCGCCGGTCTCCATGTTGCAGAAAACTTTCATGGCGTCCAAGGTGCAGCCCTGGTTG
GGATCAATCCAGTAGTCTCCTGCAGGAAAAAGAGGCAGCATCCCATGATGCTTTGCAAGACAGCCTACTG
GGAAACAAGTCAGGGGAGGCTTACATGTGGCCTAAGTGGGGGCCAGACAGAAGGGCATGGGAACAAAGAA
ATCTGCATGGAAAACCTAATGCTAAAGAGGGGTCCAAGGAAAAAGGGAGAGGCAGAGCTTCCCTGCTGGA
CAGAGTCACTTCTGTCATGGTCCTTCACCAGAACTTTCTAGGTCTAACCCCGCTTTGCACTACGGTACAA
TGGGGTGTGTCTTCCTGGCTGCTGAAGGCGGACATATTGGTGGGCTGGCTCTCTCCTCTCTGGGGGAGGG
GTCCTGCCCTCCAGGCTCACCGCTCTTCCACTCGGGGTGGCAGAGTTTCAGGTCTTGGCAAGTGCGAGCA
GGGTTCTTGCGGGAGCCGTCGGGGCTGCGGATGCTCTCAATCTGGTTGTTCAGTGACTTGAGTGTAGCGT
CCACCTCCACGTCGTGCTGTCTCAAGGTACTGTCTGCCTCGTCGGCCCGCATGTACTGCATGGGGTCGGG
GCCCTTCTCTCTCTGCCCTAAGCCAGCAAAGGCTGACATGTCGATGCCAGGACCAGGAGGACCTGGAGGG
CCAGGAGGACCGGGACTTCCAGGGGGACCCTGAAGCAGTAAATAGAGATGTCAGCTAGGAATCACAGGAC
AAGGCCCTCCTCTGTGCCCATCTGCTGGGACTATGCTAGTCCTCTTCTTGCTCATCTGGTGGGGACAGAG
GACAACAGGACCAAGAAAACCACAGCAACTTCCCCACTGCCTCCCTGGCTCTTCTGTTTCCCAGAACCCC
TCATGAGCCCTGTGACCCTCAGCTGGCCCTCAGTCTGTAGGATGCCTTCCCCATTAGTTGCCAGCTGATG
TTCCAAAGGCCCTATAAAATGCTGGGGATACCTCCGATGGTGGAAGGGGGAGGGTGGGGTGGGGTCAGGA
CACTTACAACAGGGCCTGTTTCTCCTGAGCGTCCACGGGGACCTGGAGGCCCGATGGGGCCAGGGATTCC
ATTAGAGCCATCTTTGCCAGAGGGACCAACAGGGCCAGGTGGACCCTATAAGCCAAGAGAGAGGAAATGG
TAGGAAAATAGCCTCTGAGTAGTGTAGTGGAGCAGCTATGGCTAGGTGGGTGGCCAGCCCCATGGTGCTG
GTCATACCATTCTGTCTCTGATCCCTGCATTCCTCAGCCACACTCCCTAAATGCAGCTCCATTTAGCCAA
AGGACTAGCAGGTTAGTTCCATGTCCCTCCTAAGTCATGTTTATAAAAATCTTATTTTATCAGGCTTGTC
TTCCAGCCAGTGTGACCTCAGATGTTTAGGCCACAGCCAGTGCAAGAGAGTCCCGCTTTTCTCGGGCTAC
CTCCTGGAAGGACTGCTAAATCCAAGGTGCCCTGAAGAATCAATGCTCATATCCATCATCTCCTGGCTGA
GGCCTTGGCCCACCCAGCAGAAAAGCTCATTCCACCCCCTGAAACCATCACACTTACTCTAGGGCCAGAA
GGACCAGCAGGGCCAGAAGCACCCTGATCTCCAGAAGGACCCTGTGGAAAAGAAGAAAACAGCGGAGGTC
AACACAGCTGGGTATAGAAATACATCTTCTTCAGAATGGGAAACAGTCACGTGCACCCCCCCCCCCTTGT
ACCCACTAAGAGATTCCAGTGCCTTCTGAGAAAGATGCTAAGCCTTTTGGGTAGCAGGAGAAGCTCAGGG
GGTCTGAAAAGTAGGAGGTCTTGGGCAGCCCCCTCATGGACAAATGACATGCAGTGACACCCACCGGAGG
GCCAGGCAGACCCTGCAGTCCAGTGAAACCTCGGTGTCCCTTCAGTCCCCTCTCGCCCTGCTCTCCAGAT
TCTCCTTTGTCACCTCGGGGGCCTTGAGGGCCCTATGAATGAGAAAGTCACAGATTTGGTCAAGTCTGGT
CCACTATGTGTCTCATTTGAGACCTGGTGTCTCTTTTCCAAGGAGGACTCCACACAGGATTTTCCAATAC
TTACTGCAATCCCACGGGCTCCAGCAGGTCCTGAGGGACCCATAGGACCTTGTGCACCCTGTTAAGGGGA
GATCGAGAAAAGCGTCAGGGAATGAAGTGTCAGGACATGAAACCCCTCGTCTACATCTGCCTCAGGCCCA
GGAGGCCCCAGACCCAGGGGCTACCTCTGGATACTCAAAACAGGAAAAGCAAACACGGAACACTCTCTAC
CACTTGGTCACCCTCTTCACTCCTAAACTCAGGATACTCACAGCCTCTCCTCTGTCTCCTTGTTTGCCAG
TTGGGCCAGCAGGACCAGGAGAGCCTGGGGGCCCAGGAGCTCCAGGGGCACCCAGTGCTCCAGTCTCACC
ACGATCTCCCTGTAGGAGCAAAGTGTCAGATTCAGCAAAGAATGTTTCAGAAGAGGCAGGCAGAGATACT
TTGTTTCTAGAGTGGCTCATTAGGCCCAGCACAGTTCCCGGAACCCCCATCCCAGACAGACGCCAGTCAC
TTACCTTGACTCCAGCTGCACCATCTCTTCCAGGGGGTCCATCAGCACCAGGGCTGCCCTGGACAAGGGG
ACATGAGGATTCACTTAGAGCCACTCTCTGTTTACTCTCCCACTCTGGTCTGAAGCATTGACCTGTGCAC
TTCTGCCCAACTGAGACTTCGGGGCTAACACGTCCACCAAGCTTCCACCCACCCTCCTCTTTGTACACCC
AGCCCTGGTCTCTCTGGGTGTCCTTCCCTCAAGATCATCCTGCCACACCAAGCCCTAGGGCTCACCTCTC
GTCCAGGTTCACCTGCAGGCCCTGTCAGGCCAGGAGGCCCCACAGGACCAGGAGGACCTCTGTCTCCAGA
CGCGCCAGGTGCACCCTGCTTGCCGGGCTCACCCTGAAAGGATGCAGACAAGGACCATGAACACACATGC
TAAACATCCCTGCCAGGGCTCCACCCCACCCTCTTCAGCAGAATCAGCCAACAAAGGGATGCTCCCAAGG
GTGCTGAGCTAAAGGAAGACCTAGGGTACCATTGCAGCAAGAACACCCAGTTAAGAGTTTTGAGCCTGAA
AAGCATCCTTCACTCACCGATGGGCCGGGAAGGCCGGGGAATCCTCTCTCACCACGCTGACCAGGCAGAC
CAACAATGCCCCTTTGACCAGCCAGCCCCTGGGGACCTGGAGGACCATCAAGACCCTAGGAGAAGAAGAA
AGAGCTGACACAGGGCTTGGCCTGGCTGTGACTCTTCCCACCCATTGAGTCCATTCTGTGCGGCTCCCTT
CCTACAATCCCCCACAGCCCTGCACCCTCCTCCTAGTGCTTAGAGGAGCTCAGGCGACACTCATAGGCCT
AGCAGGGGACGTACAGAGGGACCATCATCTCCAGGTTCTCCTTTCTCGCCAGGAGCTCCTGCAGGACCTT
GAAGACCGGGGTCACCAGCTCTGCCAGGGGGGCCACTGTCTCCTCGAACACCTTTGGGACCATCTTTTCC
AGCAGGACCAGGGGGACCGGCGGGTCCAGGATTGCCCTAGGAGGAACAAATACAGAAAGTGGTAACTTGC
CGTCCTCCTCATCCAAGTTACCGAGGCCACTGTGGCCTTGGGGGCCAAGGCATTCCACACGGTCCACCCC
TGTGTAAAGCTCTGAGCAGCTAGCTGTGGCTCCATAGTCCTTGCTTAGCCTTTCTTCATCCACTTGGAGC
TTTTCCTGCTGGATCAGCTTCATCCTCTATGACAGATACCTCCAGAAACAGCTCCAACCTTGACGCTCTG
ACTTGAGTCTGCTGTTCACGCAGCAGCCCCTCCTCCACTCTCAGTGTTGATGGATGGAATTTTTCCTTAT
CTCTTCTCACACTGAAGTCCCCCCCTCCGCCCGCCATTATCTGGTCTGGGGCTCATGGGCTTCTTTTACA
GATTGTAGATCTAGATGCACTGAACAGGTTTAGGAAATATGTCCCAGGTGCAGGGCATCATGCCAAGTCC
AAACCAGCCAGGAAGCCCCATTGCTCCCAGCTACCAATGGTCAATTTGGTAGGAATGGAGAAAAGTCAGG
GCCATACGGGAGCTCTTTTGACCCCTATGTTTGGAGGTTTGCTCCAAATGCCTCTGACCCTATCTGGGTC
TCCCACGCCAGAAGTAACCTGGAATGGTACTCACATTAGCACCTGGGGGTCCAACTCGGCCAGCAGCTCC
AGGGAATCCGGTGGCTCCCTGTGGACACAGAGCGGTGGGTGAGAGAAAGGCCCTGGATACCTCGGGTTCT
CAGCAGAGAGCAGAGTCAAGTTGGTAGGACTAGAGAGCTTGTTTCAGAGCTGGGGAATCAAGAAAAGTTG
AGGACTACAAAGTAGCAAGTGAGTCACTTGAAAACAATGAACACTGCACCTGGCCACATAGTCAGGCATG
GGGTCGGGGTGAGAGGTGTGGCTAGCCTTCAGGGAGCTGAGTGGCAGAGCTGAGGGCTGAGGACTCTAAA
GTGACTGTGCGCACACAGCAGAGTTAGATGCCGCCTCTCCTGCCAGGCTGTAGTCATGTGGGCTTAGCCT
GGGCATGTTAAAGGCTGGGCACAGAGAAGGTGGAGAATCCGGAACCTAACTAACCCACTGCAGAAATCAT
GGCCCAACGCCTTTTTACATGAGATTTACTCACCGGGGGACCTTGGGCACCTCGGGCTCCCTTAGGACCA
GTCACTCCAGTAGGACCCTGTGAAAGGAAGATGACAATGAGGTCTAGTGGACCTGAGGAGAGTGGTAAAC
ATCTGTCTGAACCTTAGCGCAGGACCCCTGAGAACCGGGCTATCAGAGCACCGCTTCCCAACACTAGGAC
CCCAGACAAAAGCTATCGTAGGTAGATCTGTGTGCATCACCACCGGAAGACTCTAGGGCTGGACTCTGTA
AGCCTTCTACAAATACAACCCCAAAGTCTCCCCTCCTGGGAAACCCACTCTCTCTGCTGCCCCAGGATAC
CAACTGCCTGTCCTGGCCATCTGAGGTCAGTGGACACAGCCTATACTCAGTGCTGAGGACCTAATACCCA
ACCTGCGTGCCCCACGCACCATTGTCCCTGCACAGCCGAGGTGCCCACGAGGCTCCCAATTAGACTCTGC
CTGCATGACCTCCCAGGGTCCTCCTCCTGTCCTGGCAGGCTCTGCCTCATGAGCTCAACAATACTCACCT
GTGGCCCAGGGGCTCCCGAGGGGCCTTGTGGGCCGGGGGCACCAGCATCTCCTTTCTGTCCGGCTTCTCC
TTGATCACCCTTGGCACCAGGCTGGCCATCAGCACCCTGTAATAGGAAGAAGAGACAGATGAAGGAGAGG
CAAGCTGATCACCCCAGGTCCTGCCAAGAGGTTCTGGGAGTACATGTGTACGCGTCTACCCTGGGCCTGA
AAACTGGGTTTAAGACTGGGCTGTTCTGTTGCCATAGAAATATGAAGAAAGTCCATTTCTCCCTTTACTG
CCCTCTGGCGCCCCCTCCTCTCTCCCACCAGAAGGTGCCCTTGAGTTCTGGATGTGAAGTCAGACTGCAC
ACATGGGTGGGAGTAAGGTAGACAGAGATACTCACAGGAGGGCCAGCGAATCCAGCAGGTCCAGGTGGCC
CGGTCTCTCCGCGTTCACCCTGTAAGATGACCAGGAAGTGTCATGTCCGTCGCCTGCCTCTGTGCTCGCC
TCAGACTGGGAACCCACTGCCCAGCCTCATGCTCAACCCCTCCCAGAGAGAAGGCGTCTGAGTGGCCTAG
GTCCCGGGGAGTCAGAGCACTGGTCATGCCTGGGCCTGTGACCTCTGCCTTTCATGACTTCTCTCCTCCC
ACCCCTAACCTGAAGGGCATGGCTGGTTTCCTCACTCCTGGGGCCAGGGAAAGCCTAATTTAGAGAGACA
CACTCACCGGGGCACCTCGAGCTCCGGTACTTCCTGACGGGCCAGGAGGTCCGACTTCTCCCTAGGAGTT
AAGAAGGTAGGGATTAGAGTGGGCCCGAGGACCCAAGTGTACCAGGAAGCCATGATGTTGGGAAGAGGAC
TCTGGGTCCCTAGACCCCATTCCACATTAGGTTTCTTCCCAGTCCCAGCTGTCCCATCTATCTCTTCAAG
GCCTTGGCCAATGGCACCCTTCTACCAGGCCAACTGCCACACAAGTCACACGTTAACTGGACCTCCACCC
TATACTTCTACCACCACCATGGTAGGCAATGGCCAATGAGTGAAATTTGACTGTCTGGCAGGAGGTAAGG
GGAAGACATTCTGGGGAGGTGGACAGAGGGATGAAGGTCAGACAGAGGGACAAAGGCCAGCTGCAACTCA
CCTTCTCGCCGTTGGCCCCTGCTGGGCCTGGGGGTCCGATGGGCCCAGTCAGACCCTGGAGGGAGAAAGA
TGGAGATAAAGGGAGAGAGTCAGGCCAGCATCATGGGGTTCCTTGGGGATTCTGGGAGCCCCATACCCAA
TAGACTTTGGTGTACCCTCCCATGGTCTGAGAGCTGCCACTGATGTCTGGGCCCCAGCTCTAAAGGGAAA
GCCCCTGTCGTCTTCCAGCCCCTCTTCTGAGTGTGAAAGGTCGTGGGAACACCTGTGTCTGTCTCCCAGC
TCCTGAGTCCTATAAGCAACAGCTACTTACTCGGCCGCCATCCTTCCCAGGAGCTCCCTCTGGGCCTTTC
TCGCCAACATCGCCCTGCAGAAGAGAATACCGGGTACCTTAGTGGGTACATCAGGAGCGACTGCCAGTTC
ATCCCCGCCTGGCTAAGGTTCTGGTATCCTGTCCCTGCCTTAGGAAGTCTTTTCTGTGGGCTTTGGGCTG
GTGACAAGGGGTAACGGGTGAGAAAACAGGGAGAAGGAGGTCATGATGGCAGAGCGGTTCGGTTGGTTGG
TTGGTTGGTTGGTTGGTTGGTTGGTTGGTTGGTTTCGGGGTAGGGGTTCCTAGTTTCTCAAAGAATGGCC
ATGATCCTCATCTTCAGAAGTACAAGGACAGAGTATGAAGCCTGGTGGTTGTGGCGTTGGGGAGAGACAC
CCATCACTTACTCTGTCTCCCTTGGGCCCAGCAATGCCAGCGGCTCCTCTCTCACCAGGCATTCCCTGTA
GACCTGGAGGCCCCTGAGCCCCAGGGGGGCCATCTGGGCCAGCTGCACCCTAGGATGACAGACAAGAGGA
CTCTGTAGGGAACGGCATCTCTCTCTCTCTCTCTCTCTCTGCCCCAGTGTATACAGCCTGGGAAGCCAGG
ATCCCAAACCCCTCTTCCCCCTTAAATCCCCTTCCCAGTGCCACCTCCCGCAAGTTCCTCCTAGCCACCA
TCTTTCGTGCCCCCTCCCCTCCCTCTCACCCCTCAGTGAGCTTCCCTAGTGCTAGGTAGAAGACACCCGC
GGGACTTGGCCTCACTTACTTTGGGACCATCAGTACCAGGAGTGCCAGGGAGGCCTCGGGGACCCTGAAG
GCCCTGAGCACCAGGAGAGCCACGTTCACCTGGGAAACCTCGCTCGCCCTGTGGAAGAGACATCAAGCAG
AAATCAGGCAAAAGTGATGGAGACGATGAGACAGGGGAGGGGCCCACAGGAAGAGATATGGGTCCCCAAG
AACCCAAGGTGGGCTGCAGTGTTGGGAGACATGGGCCAAAATGAATGAAGGCAGGCCCAAGGATATACCA
GTGCCGGGTAGCAGGACACTCACCCGAGGACCCACAAGGCCAGGAGCTCCAGCTTCACCAGGAATACCCT
AGAGAATACAAAAAAGGATGTAAGAGTGAAGGCTGCCTGCTCCCTCTGGACTCAAGAGCGTGCCTCCCAT
GGTGGCTGAGCATGTCCACAGGAGGACTGGGCTCAGACTTGTCCGTGGAGAGCAGGGAATCAGGTCTGGG
GCATTCCCAGGCTCCACAGGGCTCTCCATTCACTCCATTTCCCTTGTCTCCTGTACATCAGCTAAACAAG
GTCACAGAGCTCCCAACTCACCTGGTCACCTTGCTTTCCACCTTCACCTGGGGGACCGGGAGGGCCAGGA
AGTCCCTGAAAGCCAGAGGGACATGGATTAGCAAAGAAGGGTGTCTGGTGCCTCAGCTTCCAGGAGGGCG
CAGCACAAGGCAGACCCAAAGGAGAGAATTATTAAGGCCAGGCACGGAGAGCCACACCCTCTGATACCCC
ATGAGAGGAGTGGGCTTGCCACCCTTAGTGCCTGCCCAGAGAGCTCTAGGCATAGGAGGGGATTGCATAG
AGAGTCTGGTCCAGCCACCTACCTGGAACCCTGATGGTCCAGGAGCGCCCTGCTCGCCTCGTTCACCAGC
AGGTCCCTGCAGAAGAAAGAAAAAGTGAGCCAAGCCAGGAATTCCAAGAACCTTAGGCCCCGGGGCGTTG
ATTAGAAGCCTTCCTGCCCTGACCAACCAACACGTGGCAGGCCACTTGCTCTCCATCATCTACCCACACT
TCCCTCCACTAGGCATGTCCTCTCATCTGGGGATAGTTCAGCCCAGATCCACATCTGTCACCTCTCCTAC
TAAATGGATCCAAGGTCCCTCGTTTTAGCAGCAGGTACTCACACTGGGGCCGGGGGGTCCTGCGGCTCCC
GTCTCACCGTCTTTTCCAGGAAGACCCTACAGGGCAGAGGAGGAGGAGTTGTAACGACTGATGTCTGTGG
CGTCATCTGAGACCAGCAGATGTAGTTTTCCTTCGGTCAGGGGTTTTTAGATCAACAAGGTCTGCAACGG
CTCTCCGCTTTCACCCTTCCCTGAACTTCCGTGGTGCGTCCACCATTTGCCTCAGTCTTTCTCCACATAG
ACATCTTCCTCTGTACCTCAGCCTTGCCTCTGAAACCCACCAGCAGCCATAGACTGTCTGGGTGGATCCT
GACACAATCTAACATGTGCTCATCCACAGAATCAACTCTTGAACGTCCTCCCCACCCCCCCGTTGCTTCT
GTGAGGAATTCCACCAACACTAACACTGCACCAATAACAAGCCATAGGTAGTGGGGAGGACACTTACTCT
CAGACCAGGAGCGCCAGCCAGACCCTTCTCACCAGCTTTGCCAGGCTCGCCCTGAAGGGAGGGACGAGAA
GACCATGAGCTGGGTGATTTGGGATAGAGTGAAGTAGTAGGCTAGAGAGCTGAGGCCTGAAGCCTCAGTG
TGGTGCACAAGAGAAGGTGCCATCGAACATCAGCCCCTTCTTTTATAGTCACTGGCCTCCGGGGCACAAG
GCTCTATGGGTAATGTTGCAGTGCTGATATCTTAGGGTTTTAAGGTACCCAAGTTTACCCCAGGTCACAT
GCATTCCTTGCAGGACATGGAGGACAGAGCTTAATGACATTGAGAGGCCACTGGTCACCCACCCTGGGTT
AACTCTGCTCAGGACCCAGCTCCTTCTCCTCGAAGGATATAGGGAAAGACGAAGGAGATGAGAGAGCAAA
CTGTCACTTACGTTGGCACCTTTGGGGCCAGGGAAACCCATGACGCCAGGCTGCCCACGAGCTCCCTGAG
GACCAGGAGGTCCAGGGCGACCGTCTTCACCAGGGGCTCCCTGAAAGACAGGATTCATTCTCAGGACATA
GTCCCTCTGGCTGTATCTCCCCATCACTATTTACTAAGTGTGTGCTGTGTGGCAGGCAGGGAACTAGGTA
CCAGCCATACACATGCATCTAGGGTTGACCAACCCCACACACAGACCCAGCTACGGGTAGATGTTCCTGG
CTCAGAAGTCTCTCCTGAACTTCTAATCTTTGTCATCACTCCTCCTCCTGGAAAATGTCCAAATGCTGTG
TGTGTGTGTGTGTGTGTGTGTTTGTGATTTTCAGAGCTCAGAGTATAGGTCGCATCTGGCCATAAAACCG
ACCATGTGTCTCTTACCCCTTCACAAAATGTACAAAGTCCTAGACTGCCCTCACCCAAACTCCCTTCTCT
CTTCCCACCATCTACTGGACGATAATCCCTTATTTATCCTCCCAGGAACCTCACTCAGGCTAATAGACTT
ACAGAAGGACCAACTTTGCCTTGAGGACCAGCGTCACCAGGGCGACCGGTAAGACCCTTGGTTAGGGAGA
GAGAAGAGACCAAGGTTATTCGGGGTGGGGGAGGGGGACAGGCCCCAAATCTTCCAATTCAGGCAGCACA
GCTTAGAGGATGCAGGTGGGAAGGGTGTGGGGGTCTGTGTAGTATCCACCTTACCCTGGCTCCAGGAAGA
CCAGGTTCTCCAGGACGACCCGGGTCACCGTTGGCTCCCTTGGGACCAGTCAAGCCACTGGGCCCTCGCT
CTCCAGGGGCACCCTATACCAGAGGAGACAGGAAGTCCATGGGATGCTCTGTAACTAGGGAAACAGGCAG
GGACCAGGCCTCTCCTCCTCCACTCACCTTGGGACCTGCCAGACCATCTTGACCTGGGAATCCACGGTTG
CCAGGAGCACCCTAGGGAGAAGGGACGAGGCAGGGGATGAGGATGCTTTTCATCAGCATGCCTGGAAGGA
GTCCCACCAGGCTCCAGGGGTCTGTCCCCTCCACCTCCCACCACAGTGACTGTGGGGCGGTAGACACCGC
CCGTGAGTCAGAGAAGCTAATGTCCGTCCAGGGCCTGCTCCCACCTCTCTGGGAAGAAGCTACTGTTTTA
TGCCACCCTTGGGACAGAGGGTGCAGACAGCATGAGGATGCTGTGACCCTGGTGTTGGATGGGGAAGAGA
AGAGCCACGTTATCCAGAAAAGTCATTGGGTGTGGAAGAAAGGATGAGCCACGGAGCAGCGGGCCTGTGA
GCTGGAGCTCCTTGCCTACTTACTCTCTCTCCAGGGGGTCCGATTGGTCCAGCACCACCCGGCTCTCCTC
GAGCACCTCGTTTGCCTTCTTCACCAGCGGGGCCAGGGGCTCCTTGGGGCCCAGCAGGTCCCTGAAGACA
AGCACAAGAGGGGTGGGATGAGGTTTCCACCTAGCCTTGGGGAAAGCACTGGCCTGGTGTGCAGAGGAAG
GGGCCTTGGAAATGTTTCGGGGCTTGGCCAAGGGGGTTAAAGGGTCAGCCATTGGGTCTGGGGGCCAGAA
ACAGCCCAAGAGGAAGAGATGGCTTGGAACTCAAGCTATTCGAACCAGGAGAGGAGGCTGTGTCAGGGTC
TTCTCTACCCTACTTCACTCCCTTCTCCAGGGACAGCAGGAGAGAGCCCGGGATGGGAAGTGGCTGTGGG
GAGGGGGCACAGCGGGGGCAGAAGGGTGACCTGTGGGCAGAGCTACAGCAAAGAAAACAAAGTCTTGAGG
GCAGATACTCACAGTCTCTCCCTTGGGGCCTTGATCACCTTTAAAGCCAGCAATGCCAGGTTCACCCTAC
GTGAAAGAAAGAAATATGCTCGCACTGGCTTCTCTCCATAGAGTCTGCTCACCCTCCTCCCAGAAGCCTC
CTACGGTGAGCAGGAGGGCACAGAAAAGAGGAGCCTGTAGGTAGGACCTGCCAATCTTTTTGGGCTCTTA
CCGCCTGACCTTTGGGGCCAAGGGGACCAGTTGCACCTTGAGGACCGGGAGGGCCACGGGGCCCAGGGAA
GCCAGGGGCACCAGCAATTCCAGGAGCACCCTGTCAGCATGAACAGAAGAGAGGCACGTGAGGAGGATGA
GGAGGGACTAAGCCACCTTGGGAACACAGCCCAGGGACAATACTTACAGCGGATCCTTTGGCTCCAGGAA
TACCATCAGTCCCTGGGTTACCCTGACAAGAAGATGGTATAGCGATAAAATAAACAGCAAAGAAACAACC
CAACCATTCGGCCCATTGGGCCCCAGTCTAGGGGTCAGGGACAAGTGCTGTCTGCTTCAGGGGCATCCCT
CCTGCCTGTCTCATGGGGTGGACTACGGGTGAAGGGAGACAGTCGTGGATGTCAGGGGGAGGTGTTTGGC
CAGCATGACCATTTAGTGGTCCCAGTGACTGGGACTCCAGGTGACATGTACATTTGTGACAAGGCCACAC
ATGCCATGCCTTCCAGGCCAAAGAGATGAACTTACAGAAGCACCTGCAGGCCCAGGGGACCCAGGATTGC
CAGGCTCGCCACGAGAACCTTGAGCACCTTCAGGACCGCGAGCACCAGTGGGACCAGCTTCGCCCTGGGA
AGAGAGTGGGGGGGCTGAAGTGGGGGTGGTGGTGGCAGCACTTCACGGCCCAGTGTGCTTGACTCTTGTG
TGTGCTCACACTCCTGTGAGTGAGTTGCGTATGCATGGGGGTCTGTCTTTTCTGTTCCCACCTGACCAAG
CAGCATGAAGAGCCGATATCCCACCCCTGCCTCTTCTACCCTCACCCCTGCCCTGTGACCCCACACACAG
CTGCACAGATAGATGCCTACTGCATTCCAGCAGCAAGCAGCTTGCTGGTCCTTCAGTCCAGCAGAAAAGC
CTGGGTTAGGAGACCTGCCATCTTAGGGCCATGGGATGATGGACAGATGGGCCTCCACTCCCCCTTCCCC
TCCTTTTGTTTTCTCTTTGACAGAGGAACAGAAGCCAGAAGGGTGACTCTGTCTCCTCCAGAGCCTCAGC
TCAGAAGTGGCTTTCTTTATGGCTGCTGGGTGATCAGAGCTGCTCCAGGCTTGAGCCTCAGCCTGTTTGA
AGTGCCAGGAAGCCAGGGCTGAAAGCAAGCTGAGACAGGCTCTACTTTTCTTTAAGTTAACTGGAAGCCC
CTTATCCCCCATGTAAAAATTCTTTTTCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT
CTCTCTCTCTCTCTCTCTTCCTCTCTTATTTTTCTTCTTTCTTTTTCCTAAAAGTCACAGCTCTGTGATC
TGGCTCTCTGGACTGGAGCCTCCTCCGGGGACGGCTGCTGCCCTGGAGAGAGGTCAGAGCCTCCTCAGGT
TTCTCCACACTGGCATTCTTACTTCACTTTGGTAATTATTATTTTCTAAAGGTCAGGCCCCCCTCACCTT
CCGCTGTCCCTCTTCACAATGGGTCTCCTTCACAGGGCTCCCAGGGGCCTCAGACACTGTCTGGTTGCCA
TGGAAACCCCCAGGCGTAGAAAAGCAACCTGATGCCAGTGACACAGGGAGGCTCCTTACTCACTCCCAGG
GACGTTCACACTGGAATCCCTCCCGGGCCACAGGGGCTTTTCCTTGAGCCGGCTGGCCTCCCGCTGGTCT
CTATTAATCCCTGGAAGGACCAGGTTAACAACAGCCCAGCACCCCAAGTGAAGTAGAAATAAGAAAGGGG
CAGGGGATGCACAGAAGAGTCGCCTCGCCTCTGCCCCTCCCCCTCTATCCCTTTAAGCTCCTTCTGGGTA
CCTCAGCTTTTGCCCACCCCCAGGCTTGTTTTCTTCTTCCTCCCCTCCCTTAGTCTTCTTCTCTCTCTCT
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGGGTCTGGGGGGGGAGG
GTTCCCATCTTTGACTACTAGGTGGACTTCAGCCTCTACAACCGAACCAAACTCTGTCAGAGAGAGATGC
TGGAAACACACAGAGAGGCTAGGGGGGATGTGTGCCAACAGGAGCCTTCCTTAGCAAGGACCACAGGAAG
ACTGGGTGTCCTGACCCTCTGAGATTCTCAGCTTGACCAGCAGATCACTCACCTTGGCACCAGGAGCACC
AGGGAAGCCAGGACCACCTGCGGGACCCACAGGACCCTGGGGAGAAGAAGGGGACAGAGGGTGAGCGCTG
GGTGTCAGTGAACTCTGTCCAGGCCTCAAAGAGAGACGCCCCACCTCCCACTCCTTCCACCTCTGTGGCT
AGAAGAAACTGCCAGGGCTGCTCTCCCCAAAGACCTCCAGAGAGACACCCTGCTCCTCTTGGAGAGTTTC
TGAGTCTGTGAGAAGCCACGGGGCTTTGACCTCTCTCCTTAAAAGAGGAGACTGCTAGGGGCAGCAGGGG
ATGTCAGGCTGCAGTGTCAACAGCATGCTCACGGGATGAGCCACCTCCTTCCTGCCACAAAGTACAGGGG
CAACCCCACAGACTTCAGGGCTGCACAGCTACTGTCACCTGCTTAAGAAGGACAGCAACTTACCGGAGGT
CCAGCGGGGCCTGGCTGGCCATCGTTACCCCGAGCACCCTGTGAACAGCAATAGCACAAAGGAGCCAATT
AACACAGTGTTGGGGTGTCCCAGGCTGAGCATGAACAAAGCAGAGGAACCTTCCTGGGATATCTGTACAA
GGGGGCAGAGAGACAGACAGAGAGAGGGCTACAGGGCACTGGCCCTCGTGGGTAAGGAAGCTTGGGCAAC
TTCCCAGCAGACCTGTCACTTCTAAAACTTCAGTTAGGTCCCCAGTATTCCCGACTTGAAGCTGAATGAT
CATTCAAAGAATCCTGTGGGCTGGGCCAAAGGAACCGGACAGAAGGAAGGAGGAGCCTGCCCCTGTGCTG
GGTATCCTGGCTTAACAGAAGCAAGTCTTCTCTACCCTCTGGTCCCTCCCAGTCAGCTGCTCTTCAGGAC
AGCACAAATACCCCCACAATGTTCCCTGCTGACCGGGAACCTGACCCGCGATGAAGACAGACCCTGTCTA
CAACCGCGCCTGAGCTCCTATCTCAGAGACCCTTGGCTTTGCAGGGGTGGGGGTGGGGGGTGGGGGTGGG
GTCGAGGTTGTCCCTTCTCTAAAAGACTCGAGCTCCGAGAGTTCCACTGGTTTGGGGTGAAAACATCTTT
CTATTTTTCTCCAGTGCCCTTGAGAATTTGTACTCTAACTCCAAGAACCGCTATGTTAGGCACATCCAGG
TCTAGGACTGGGTCCCTCCCAGACCCAAAAGGCTAAGCCAGTGGCTCTCAGCTTTCCCAACACTGTGACC
CTTCAATACAATTCCTCACGTTGTGGCAAGCCACAATCATAAAATTATTTTCATCGCTCCTTCACGACTG
TAATTTGGCCACTGTTATGAATCATAATGTAAGTCTTTTTGGAGATAGCGGTCAGCCAGGGGAGTCACAA
CCCACGGGGCTAAGGGGGGACTTTCTGCACGTCTCTGACTCTCAGAAGCACAAGGCAAGGATGCTCTGCG
GTGCTCAGTCTCCTTCCTGGTGTGAGCCATCTCTCTTCCCAGAGCCTCTAGCCTGGCAGCCAGAGGGAGG
GGCATGGCGAGACAGCAAGACTCAATAGAAGGAAGAGAGAAATCAGAGCCTGATTTCCTGACCCAAGCGC
CCTGGAAAACTCTGTTACTGGGGAACCCTACAGTACCTTCCCTCCAGGAGTCAGCAGAGAGCAAGGCAGG
GGGAGAGGGCCCAGTCTGAGCTGCCTGTGTCTGAGGCCTACATGGACAACTTCTAGGCTCAGAAGGCCTT
TGGGAGTCCTCGCAGCAAACAGCAAGGCAGAGTGGGGAGCCCCGCCCCGCTCCCACCAAAAGGCCCAACA
CACATGTAGAGAACATTCTAAACAGAATCTCCTAACAGCGCTGCCACTGCTTTGTGGACGAGACCACCAA
GGAAAACCAAGAACTGGGGCTTGTGTCTGGGAAGAAGGAGAGACAATGCGAGAGGCAGTGAGTGAGCCAG
TCTGGGTTTGAGCCTCGTTAAAATCCCCAGGCCAGGGTCTCCCGACTCTGCAGAAGCAGCAGGCCCAGAG
CTTCCTTCCTTTCATACAAAGCGCCCCTGGGGCGAGGCCTTTGGCCAGCTCTGGTTGGCAGTGCGAGTGC
AGGCTGCTGGTTTCACAGACCTGCTCCTGAGGGAACTTTCCCGCCCAGATGCACGGCCTGGAAAGCAGCT
GCTTACAGTTGCCCTGCAAGGACCTAGGAGGAGGGGCCTCTGAGCTTGGTTGGCTGGGTGTAGACCAATA
GAAAGCCGTAAAACTTGGTTGGCTGGGTTGCAGACCAATAGAAAGCCGGAATCTTTCTTAAGCGCGCCGT
TCATCACATCACTCGCCTGACCAAGCACCTACAAGGCTTCTCTATTGCTTGGGGAGCCAAGATAACGGCC
TCTCGCTGGCACCCATGCCCTCCACTGCCTGCCAGTCCTTTAGCTCATGGTTCTGGGCCCTCGGTACACA
GTCTGTCTTAAGCTAGTTATTCTTCCCCGGGGACTCAGTTCCCTCTTCTCTAAAATGGGAACGAGAGATT
CAAGTTCACAGAATGAGGGGGAAGATTAGACAAAGAGAAGCAAGCCAGGTTCTAAATCAAGGGTCACGAG
GTGGATGCCGCTGGTGTCCTATCTCGACACTACTCAGAACCGCATCAAGGGCGCGGAATCGAGAGAGAGG
GCAGAGTGTATAGCATAGAGGGCTGGCACCATCTAAGCCTGATCCTGAACTCTAACATGCACGGCTCAGG
AAGGAGCAGAGGCTATTGAAGGGGTGACCTGAAGGATCCCGACTCCGGAAGAGGACTCTCACCAGCATAC
TCTCCTATAGTACGCCCTCCTTCCACCCTGCAGGCCCCACCCCACCTGAAGATTGGGCGTAGGAAACCTC
AGGATAGAAAGTTCTGGTATGATGATGCGCCCTAATCCTCACCAAGTTGGATTTTGTTGTTTATTAGGTA
TTGGGGGGTGGAGTGTGGAGAAAGGTCTTATGTGTGGCGCAGGCTGGCCTTGAACTTATGATTCAGGAAC
TCAGCTTCTTGGGTACTATGATTATAGATAGGTACGTCCCTCCTGGCTGGGGTTACCACTGAGTTGATTT
GAGGGTCAAGAGAAATTTACTTGTAAGCTGTAAAGTGTTGAGCGTGTGGTGGCACCGGGCTTTGGGGGTT
ACTCACAGCAGCACCAGCAGGGCCAGTCCGTCCTCTCTCACCAGGCAGGCCACGGGGACCCTGCAACACA
CACCAGCGCGGTTTAAGTGTGAGTTGGCATAATGATGGTCTGCGTCATGACTTCTTCCCACCCACGGTGG
CCCCATCTCGACCCTAGATCCTGCTCCCAGGACACAGGGGTTCCTTCAGGGAGACCCCTGTGTCTTCCTG
CCCCTTCCTATGGAGAATGCAGCTCGGTTCTCAAAACAGATGAGTCACCCAAATCATTGAAGACAAACAG
ATTTTTTTTTAAGAGTAACTGAAATGTGCTTTAAACGTGCCTAACTGTTCCTGTTTCGAGGAAGGGCGTG
TGAAATGTTGAACAATCTACGCTGCAGGTTGGGTTGGCTCATAATCGGATAAATCTGTGCGAACAGCTTT
GCATGAGTGCATGAAGGAGGAAATGCTGCTGTTTGCAGGGTAGCTGTCTGACAGGTGTTTCGGGAGTCTT
AAGAGTGTGGGGTGGCTTCCCCAGGGGTGACTCTCATACTCACCATTGGGCCCGGGGATCCGTTCTCACC
AGGGGAACCACTCTCACCCTGGAGAAAGAGACATATAGGGTCAGTGTCTGGAGTCTTGGCTTCCAGAAAC
TTCCTAGGTAAGTCATCGATAGGTCTGGCTGGCTCAATTAGGCCTGAGACATGTCAATTTCAGTCCGGGG
AGAGACGATGTCCCCAAACTCTAAGGCCTGCTGCTCATGTACCAAGATGTAGACTGGGTGTTCCCAGCTA
TGTTCTAACTCCCGACCCCATCAGCGCCGCCTTGACTGGACAGTTGTCCCCTACCGCCACCCCCTGTGTG
TCTTGTGGCCCCTTTACCTTCACACCCGGAGCACCAGCTTCCCCCTTAGCACCGTCGAGGCCTGGGTAAC
CCTGGGAAACAGGACGAGGGTTCCGTCACATGTCTGGGAGCCCACCCAGGAAATAGTGTGCATAACCCCC
AAACATCCTATCCCATGATACTTACTCTGTGACCCTTGACACCGGGGAGACCCGGGGTTCCTGGGAATCC
ACGAGCACCCTGCAATACCAAGAGGAGGTACTCAGAGGAAAGCAGAGATTGGGTGAAGGCTGCTGGATAG
ACTGCAAGTCCAGCTCCCTCAGGCCTACAGAAACAGGCATCCAGGTGTGTCCACAGCTCCTTCAGCCCAA
CCCAGAGCATCACCTTGTGATCATACAAACACGGTATCCAGCGTGAAGGCATTACGTACACAGTACCGTT
AGTACCGTGAGCTAACCAAGACGAGTGAGCCATATGGACTCAGAGGGAGACTGGAAAAGCCTGTGAGAGC
GAGGAAGATGAAGCTTTACCTGAGGGCCAGGGAGGCCTCTTTCCCCAGACTTTCCGGGCTTCCCAGCTTC
ACCCTGAACAGAAAGAGAGGCATCTCATTTTTCTTGGCAGTCTCTTGAGCGTATTCACTCCACGGCTGGG
CATCTGCCTATCTGCCCACCTTGACCACGCCCTTCCTGCAGATGCCCGTGACCCAGAATGCCTCTGTGGT
TTATCCCTTGGCAAGTTCCTGGGCCCTCACTCCACTGTCCCCAGCAAGCGTCTGGTCCCCTCTCCAGAGC
CCTGTGGACTGGCCTGTATGCCCTGATCTCCAGAGGCCTTGCTGTGTGCTGCCAGTCATAAAAAAAAGCT
CAAGAAAGCCAGCTCTTCTCTCTATTTTTTTATGTCCCCCTCCTCTGTAGACATTCTGGCTCAGGCTGAG
TTTGTCTATCTCATTTACAATTGAGATGATGGTAGCCTGTTTGTTGACCGACTCTTGCTTTGTTTTTCTA
CTCTATACCGCTGTGCTTTGAGTCCTCCCATCCCGGCTGGCTCTCTCCTTTCTCATTTCTCTTCCCGAAG
TTAATGATGTTTTAATTACTCTCCTTCTTGTGGGATCTAAAGGGATCCCCAGGGATCTGGCAAGCTGAGA
TGCAAATAAAGGATATGTGGGTGGTAGATAGCTGGAATTTAGGACTGGCTAGCCTGGTTCCTCAGGACGT
CTCTCTACCCCAAGCCCCAGCACATCCTCTCTCTGGGTCTGGGGCTTCTTGGGTCTACTCACGTCGTCAC
CAGGTTTTCCAGCAGGGCCAGGAGGACCTCGGGGACCCATGGGACCCTACAAATAGAGAATTCAAGACCT
GGTTGGAAGGGAAACACAACTCGAGGCGGTCGCTTTGGAAGCTAGTTACTCTACAAGGAAGAGAGACAAA
AAGGTAGTAACAAAACTGTAAAGCAGATCATGGTGGGAGGAGGGGACTGCGCAGGCTGGCGACCCCTAAG
GGGAAGTGTCCCGATGGAGTTCCTGCCATATTGATTCTCTGCCGTTGTGCCCCGTGGGTAAGGCACTCCC
ATTGTGGGAAAGGGCTGGCAGAGCCTGGGAGCAGACGGTAGCTTGGGTAGAAGATTGTCCCCAGAGCCTC
CTGGGAGAGGGTAGCCTGTGGTACTCACAGAGACACCAGGCTCGCCAGGTTCACCAGGATTGCCTTGAAA
TCCTTGAGGGCCCTAAAAGGGGAAATAAGGACGCCATGTTGACCTCCAAGCTGTGCCGCCGTCATGTGGA
TGATGAGCATCTACAGCGCCCCCAAGAGGAAAAGACTGAACACTTACGGGGGCACCGGCAGGGCCTGGGG
GTCCACGGGGTCCCATGGGGCCCTGCATTGGAACAGAAAAAACGAGACGCTTACTGGCCCGCTTTATCAT
TGGCTTCTAGGATTCCATCTTAGAAGAGGGACAAGAAGATAGTAAGGGAGCAGGAGGGGGAGGGGCAAAG
GGACTCCAGCGGCTGCTCCCTCTTCTTTCCCACTCCCCACTCAACCATCTATCTTTATTTATAGGCCCCA
TTTGGACCGAGACGCTGGCCTTGCTCCCTCCTGGATGGCGGCCATGTTTACTAAAGTCTGAATTTCATTT
AGCATGTGCCAAGCCATCGCTGCAGGGTGAGATTTCCTGCTGGAAGCCTGGTGGATGCTGAAGAACAGTC
GCTGCTGTCTGCATTCTTCAGTTCTCCTTCCAAGAGCAGTGGCAACCAGCCCCCTAATACATCGGCTCTG
TGCATCACTCCGTCCCCCTTCCTCCTAGACACACTAGAGCGTCATCCAGACTGGCATCCATCACCATTAA
AAACCAAACTCCCCAGGGTTAAATGAGAGGTTTCCCATAGCTGATGGAAATGAGATGCCCTAAATCATCG
GCGAAGGCAGAGGTTGCTGGGTCTCTGGCTTTGCGAAAGGTGCCCTCCTCCCGTCCGGTCAGCACAGAAC
TGGAATCCTACCATGGGCCCTTGCATGACTCCCATCTGGGCACCACCAGCCTTCTCGTCATACCCTCCAG
CCATCTGAGCCGCGAAGTTCTGCAAAGCAACAGAGCCGTGTTAGGGGGCTGAGAGGCCCTGGTGTCATTC
CTAATTGCTCGGAGTTCTACCTAGTCTCTGGTTATCAGGGACTAGCCGAGCAGCGTTGGTCAGAATTGCC
AAAGGGATCTGACAACGTTTTGGGAGTGTGCCCTTGCGACAGTGGTAGAATCACTTCCTTGGAAAGTCAA
GTGTTATTTGTGGTGGTCCCGGTCCCTCACGCTCTGCCTTATAAACGTTCATATTCTGAAACATTCATAA
GTAAAACATCGCCTTTAAAAGGCATCCTTTGTTCTCTCTGATGTTGATGTCTGGCAAAGGGCTGAAATCT
GGTCTTGAGGTATTTCGGGAAGGGTTATAGTAGGCGTCTGTGAACCCGCTTAAGTTGAAGGATTTAAAAC
ACAGCAGACCTCCAAATGGCACACCTACAAAGTGGGCAGTCATATAATTTGTTGACTGGTGTGATTCCAA
ACACGACAGTAGCCGCACACCTTCACTCAGTGGGGCTCTTTCATAAGAATGAATAGTTGCTTGCAATGTA
CTTGGCAAGGGCTATGGACAAATGTCTTCCAAGGACAGTGGTCACTGCTCTTCCAGAGAAATACAAGTGC
CCAGTCATTCTTTGCGGGCACCCCTACACCCTCCCCCAAGAATTAACCGCATGGTGGGGTGCCAGGAAGC
CAGATAGCTGAAGGCCTTCTTTACAGAGACCCAGTGAGTGAAAGAAAGTCAGCCCTTAGCCCCCAGCGCC
TCGAGCCTCATACACAGATCTTCCTTAATTACAGGCCGTGAACAGGCTGTGTGCGTACTGCTGGCTGGCT
CTGGGAGGCTGCCAGGTAAGAGAGTAGCAATTTAGAAGCCACAGTTCTGGAGGGTCAGCCTGGAGAAAGG
AGAACTAAGGGCACTTACTCCTGCACTAAGGCCAGGGGGACCAGGGGGACCTGGAGGGCCAGCGGGGCCA
GGATTTCCAGGGGTACCAGGTTCTCCATCTCTGCCACGGGGTCCAGGCGCACCCTTGACAGGAAGAGAAA
AAAAAGTTATCAGTGGAGCACCGCTACCTTTGGAACCATCTGCCCTGGGCAGCACTTGGGAGGTAGCCAG
GCCTTTAGACGACCCAGGGGACACAGACATCAGTATTTGTTGCTGTCTGCTCACCTTTTCTCCCTTGTCA
CCACGATCACCTCTGGGTCCTTGTTCACCTGCAGGTCCCTGAAGGTGAGGGGCAAGGTGTGTTGACATGA
AACTAGAGGTCATGATGGGTCAGGGGCAGTCAGAGAAGAATGGAAAAAGAGATCTCTCCCTTTCTTACCT
GAGGGCCAGGAGGTCCTCTGGGTCCTATGATCTGTGTCAGAGAGAGCCATGAGCCCATTGGTTAGAGGGG
TGCATTTGGGTCAGCAGGTGCACGGGTTGTGGCGGAGGTGAGTCGGGTGGGGACGGGTCACGGTGGGGAT
AATTTGTACTTACATCTCTGATATCTCCAGGTTCTCCTTTCTGCCCCTGCATCACAAAACATCGACAGGT
GAAGGCTGAGCAAGCCCGCTGAGCCCCCACACAGAACCCCACCCCACAACCCTAGCCTCTCAGATGGACA
TCTGAGGGCTAAAGAAGAGGCACAGACCAGATTAGTATCTCAGTACCCCTGAAGAAGGACACGACACATG
AAAGCAAAACATGGACCAGTGAAATGGCCCGGTGGCTAAGGCTCTGACAGAGCCGCCCTAATGCCCTATG
GTGGGAAGAGAGACCCAAGTCTTCAATGTTATCCTCGGGCCGCCACACATGCGCCATAGCACGCTTGTGT
CCACGCTCATACATACAATCGCGTGGGCAGGGAGTGAGAATGTAATTAGATTAATTAAATTAAATTAAAA
ATGGGGTGCCTTACCTTTGGCCCTAATTTTCCTGCAAGGGAAGACAGAGAAGGAGAAGTGAGACAGGGAT
GATAATTTGACACTCAAACTCTCTTCGAAGGATCAGAAAGGAAAGGGCCACAACCGAGGAAGGAAGCGCA
TCCCTCTGTCCCCGGAATCCCTCTGTGGTGGGGTTAGGATCACGGAGCCCTTTTGTGGCTGGGGACTTAG
AAGGGACAGAGGTGCCTCTACCTCATTAGTCAAGAAGCATGCATAGTGTACAGGAATATGGCGGAGAGCA
GGGGAGGAGGCAACCAGGGGTTGGAGGGCAAAGCGCAGAAGACCCAGAGGTCAGGCAGTCCTTCCCAACC
CGTGCCCTCCCAAAGAAGAGTCTAAGGAAGCCAGCCTTACATTAGCGTGGAGTCTCCTTGACGTAAGCAC
AAAAGAGAGCGGGACACAGGTGTTACAAAGAGCATTGCAGCCAGGGCCATCCCAGACCCATACGCCCCTC
CCCCACCACAGCAGCAAGCTCCACTGGGTCCTCACACCGCACTGGTATGGGCGCCTCCCTCTGAGAATCT
GCGCCATACCAGGCAACAGTGTGGGGGAAGCATGCAGGAAAGGCGACTTCTCGATTCCAGTTCAGCCCTC
TTCTCCATAGGAGTAGGAAGGGAACCATGAGCCTGTGCGGGCATCCCCCACGCTCTCCGTAGGGAGATGG
GACACTTGCGTGGGTGCTGGGAAGGGGTCAGCTGTACTCTTCTTTGTTAGCACTGGTTCTAGCTGGGAGT
GGGTAACCACAGCAGCTTGCAGGGGCACGATCATCCTTTCAACTCCCAGAGCCCAGCTACCCCTTTGGCT
TTTCTTAAATTAATTAGCCGACTTGACTCAGGAGGGAAAACTTTTGTGGAAGCATAATTGTGTTTCTGTA
CGCATCCTCCTGGGGTGTTAGGTGAGGGAGGGTCTCTGTAGTGATTCAGGCTCACCAGCCTTCAACCTGG
TGGAACCGAGAAGTGGCCTTGCCTTTGTGCCCCTGCTGGTGGGATCAAATAGATTGATTAGCAGCTTCTC
CGCTTCAAAGAGAAGGCCAAAACCCACGGAAGGCATTTGCTGTGTCTCCGAGTAATTTATTTATGTTGAA
TAATAAATAAATAAATTACGACCACTGGCAGTGGCGAGGTCAGCTGGGCAGATGGGACAGCACTCTCCGA
AGGGGATCTCGGGGTTGAGGCAGTCTGGGTCTTCACAGATAATGTCATCGCAGAGGACATTCCCAGTGTC
ACACACACAGATGCGGCAAGATGAGGGCTTCCATACATCCTTATCTTTATACCTCTGCCCATTCTGCAGA
CAGCTGCCAGCCTCCTCTGCACCAAGTGGGGTGGGAGGGGCGGCGTGCCAAGCAGGAGCGGGGAAGAGCC
AGAGAGAAAGAGAAAGAGGTTGTAATAAACTTGACATAATTAGTCCAAATGCTAGGGAATGGTGGGGGTG
AGGTAGCAGACCACACCAGACAGGCTGAACTGACACAGTAGTTTTGAAAAACATAATTTGTCTTTCTATG
TATTCTACAGTAGCTATTATGTTTTGCACTGTGCAAGACTGGTATGTTGGGTGGAGTGGGAATCGTGAAC
CAGCGATAAAGGAGGGCTGTTACTCCATTATGTCTCTTCTTCCCAGCATTGGGGGAGGGGGAATGGAGAT
AGGGAAGGGTAGTTGGTGAAACTAAGTGGCAGGGGGAGATGATGCTCTGAGCAGGAACCCTGGGGCAGTA
AAGATGGAGAATACAGAAAAAAAAAAAATAGCGGGACACTCTAACTGCAGAGCAAAGACATCCTTTTTGT
CATCCTCTAGGAATAACAAGATTTTCTAAATATAGCGACAACTAAGAATCAGAAAGCGCAGAGAATTCTA
CCTACAAACGTTAGAAGACACAAGCAATACATAATAATCTGAAAGGCAGGCCGAGCCCTCGGGCTGGGAC
GCTCCTGCCAGAAGTCTTGCCTCTGACTGAAGATGGCAGCCACAATATGGGTGGCAGAAGATTCTCAATG
CCTAGTCGTGGCTAAGACTGACCAGTCTAGACCTGCGTTCTCCAAGAATGCTAAGAAGTAGCTCGGGCTG
GACCCTCTCCACCAGCGCCCTGCCGTCTGGAATTCGCCCTGCACATGAGAACGGTGCTGACAAGAGAGCG
CCAGGGACTGAAGAGCCCGCACCGTGTGCTAGGCAGCGCCCGGCGCTGTTTTTCTCATCAAAATCTCAAA
AGCAGTGGCAAAAGCTGAGGGTGAATAATAGCGAAGAGCCACAAACCCAGTGGGCGTGGAGAGAAACGGG
CCTGCCCTCTGCTCTTCAAGGAGGGCGAGAATCCAAAGATCAAAGTCTCCTTTTTCTTTTCCACTGGAAG
AAACCCATCTATCTAGCCTTTAGATAAGACAATCGAGGAGAAGCTGAAGGAATCCCAGGGGGAAAAGGTC
GAAGCTGCTGGGATACTCCCTGCTCCTCCACCTCAGGCTCCAATCCCACTTTCCCCACCCCCAGCGGCGC
TAATATCGCTGGAGGTGAGGAAAGGCTAGTGACGCCCTGTCTTGACTAAGAAATGGCACTCTATTGCCCA
AGACTGCCTAGGACAACCCGAGAAAATCCTGGAGAGGGTGCGTCGTGAAAGCTGATCGGGTTCCCCAGGA
AGGGCGCCCTAGGGCCGCGCGGGCCAAAGGAGGGGCGGGTCTGGCCGGGTGTGAGAGCTGCTGGCTGACT
CTTTGTGGCTGGGGCTCCGGACACAGCCCTCCAGCAGACAGACATCCGTAGAGTAAAATGTAGGATTAAA
TGACTGCCCAAGAAAGGAGCCAACGCTGTAACCAGATCGCCTAGGTGCGGTCCGAAGAAGCCAGCACCAC
CCTAATTGTGGCACGAAAGGGGCTACAGATAGCATCCTGGCCGCCCGATCCGGTTCCCCTCGTTACCGCC
GTGCGGCATAAGTGATTCTTTGTGGCCGGCCAATTAAAAAGCTACCTCTTTCGGGGAACTGTTTTGCTTC
GTCGCCGCTGTGCGCGGGGCTGGAGCCCGGGAGGTGGCGGCAGGCGGGCACGGGGGGTGGGGGGGGGATG
AGGGCGGGGAGGCTGTGCATTGTGGGAGAGGGGGGTCGGGGAGTTGGGGGACCCACTGGGCCTTGCCTCT
CATGAATGGGGCTTTTCTCAAGCGCATACAGAGCCCGATTCACAGCTCTCCACGAGGAACCAGTTTAAAT
AAATACGGGCAGGGTTTCAGCCCATCTGGAAGCCATCGCTGCCAATCCCCCAACGCAAACAAGTCTCACA
AAGGAGGACTGAGGTCTCCCCTCCCGAACCAGCAGCACTTTTGGAAGGCTAGGGCTGAGAAATGCAGATG
TGGGTCAATGTCTTCGCGGCCAACAAGTTTCCCAAACTCGTGGAAAGATGGAGAGGAGGAGCGCGGTGGA
CTCTAGGTTTCTGACATTCCTAACGTCCACACAGGCTACGCCTTGCCCCACGTCAGGTCTTTGGGTCCTG
TGGATCCAAGGCTAGAGTGTGGGGTGTCTGCCATTGGTGAGGCAAGCTGGGGTGTAAAACCAACGATTTT
TTTTGGGGGGGGGGGGCATGGTCAGATGCAGAATCGTAAGAGACCGAAAGTTTTAGACAACCTCCCCCCA
CCCACCCACACTCATACAAAGCTGTGGAATTCACTCTGTCAAAAAGGTTGCAGCAGTGAGAGAGAGCTCT
TTGACACTTAAGCGAGCGCCTCTGCTATCAGATGAAGAATTCGAGAAAGCGTTAAGACGCTCAGTTCACT
CCACTGCGCTCCATAAAGAACACCGGTCAAGACCGCAGCTTTGTGGGGTGTGTGCTTGAAAGTTTAGGAC
AAAAAATACAGTCAGGCCTATCTCAGACCACATACAAACACTTCGATGCCCCCCCACCCCCGCCCCCAGA
GCTCGAACGCAGGACTCTGGCTTAGCTCGCCACCCTGCCACCCTGCGCGGCGGGGTCGGAAAGTGCTATC
CCGGGCTTCTCGCGGCAGCGCTCCCCGCTCTAGCCCTGCCCCCCGCCGGGTCCGCCAAGCTAAAGAGCAA
AAAGTCCGCCCGGGCCCGCCTCCTTCTAAAGCATCTCTGGAGACAGCCTGGTGGCAGGGGAAAGAGCACA
GAGTCTCACGGGCAGAGGGTCTTTGATCCCCACCTACTGTCCAAACAGTTTCACCTGCGATTGATTAACA
GTCTGTAACATACAGAGAAAGACAGGGCTGGAACCCTACCCGTCGGAAACGTGCCCAAACACGTGGAAGA
AAATTCTCTACCTAATACACTTTCCAGGCTTCGGTTGTCCTGAATATAATCCCAGACAGTGGATCTTACC
CAGGGAGGAGATGGAAAGGTGCGGGAACCGCCCTTGGACCGAGGCTATACAGCTGCCACCTCTAACGATA
GCCATACGCAGGTCACTAGCCTGACCCCATATCCATCCACGGCTACACACGCTGTAAAGTAACTCATTGT
AGGGTATACAGTGTGTGTGTGTGCGGGTGGGTGCTCTTGGGGACACATTTGGAAAGCAGGTCTGTTTGCA
ATGTCAACGTTTTTCAGTGTCAAATTCCTGCAGCCTAACGGAGGCAAGGGGAGAGCCGAGTTTCAAAGAA
GGGCTGCCCGGCTGCACCTTACTGTGGGCCAGCCTGATAGCGGAGGGGACTGGAGAGCTGCGCTGAAGTC
GCCGGGAGCCGCTTTTTCTTTGTGGCCTCAGATTTCAACCCATCAGGGTCAGAGTTAGTTCTTTGTCTAG
CGCCCCTTTGAGTTCTGCAGCGTCTAACCCCTAGACTAGGCTGATAAATCAACCCGTAGGCGTCTCTCGA
TTTCTCTCCTACTTTTAAGTGTGGGGCTTCTTTTACTGAGCTCGAAACGTCCTGGTCAAAGCTGGTGGAA
GGTGATGCGTCTGTGTTCAAACCTGTTGCATATCCTTGCTTAACGCAGGGACCTGAGAAAGTCTCCAATC
GTGACTCTCACTGAAGATGCTGCGACAGAGGTCCTTACTCGCAGAATTAGGATCAGTGTACTCCTGTATA
CTCTGCGCTTAGGACTTAGCGCCCTAACCCGGACCAGTACTTGGGGGAGGGAGTAGGAGGAAAAGGGTCA
CAAGTCAGGGAAGTAGGCAGGGGCGGCGGGCGACTTACGGGCATCCTGGCCCTGACACCGTAGGACCGCG
GCGATGAGCAGCGTCAGCAGCACCAGCGACTGGGGAGCCCCGAGGCGGATCATGGCTCACCGCGAGGCCT
GGCAGAGCCTGGCCCGGGCGAAGCGCAGCGAGGCGGCAGAGCACGGAGCGGGTCCGGGTCTCTACCGCTC
CCTCATGCAGGAGGCCCTAGGAGCAGGAGGAGGCAGGAGACCCGGCAGCCCAGCGGCGCTCTGCGTCTTC
CCCCGGCAGAGGCTCCAGTTATATGCTCCCGGTCGCTCCAAAGGCTGGCAAACCGGCCCAAACCGCGGGC
CGC

Protein Sequence : Show Sequence

>NP_001106987.2 collagen alpha-1(II) chain isoform 2 precursor [Mus musculus]
MIRLGAPQSLVLLTLLIAAVLRCQGQDARKLGPKGQKGEPGDIRDIIGPRGPPGPQGPAGEQGPRGDRGD
KGEKGAPGPRGRDGEPGTPGNPGPAGPPGPPGPPGLSAGNFAAQMAGGYDEKAGGAQMGVMQGPMGPMGP
RGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGLPGPQGARGFP
GTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDG
QPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGSRGEPGNPGSPGPAGASGNPGTDGIPGA
KGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQAGEPGIAGFKGDQGPKGETGPAGPQGAPGPAGEE
GKRGARGEPGGAGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLTGPKGANGDPGRPGEPGLPG
ARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAGAPGL
RGLPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGDQGIPGEAGAPGLVGPR
GERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPPGAQGPPGLQGMPGERGAAGIAGPKGDRG
DVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGP
PGADGQPGAKGDQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPP
GANGNPGPAGPPGPAGKDGPKGVRGDSGPPGRAGDPGLQGPAGAPGEKGEPGDDGPSGLDGPPGPQGLAG
QRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGR
DGAAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIAGPQGPRGDK
GESGEQGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPG
PRGRSGETGPVGPPGSPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPMQYMRADEADSTLRQHDVEVDAT
LKSLNNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNP
ATVPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIA
YLDEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPM
DIGGAEQEFGVDIGPVCFL

Molecule Role : Protective antigen
Related Vaccine(s): Adjuvant arthritis DNA vaccine CII encoding type II collagen

4. hsp65

Gene Name : hsp65
Sequence Strain (Species/Organism) : Mycobacterium leprae
NCBI Protein GI : 34333423
Other Database IDs : CDD:295468
Taxonomy ID : 1769
Gene Strand (Orientation) : ?
Protein Name : heat shock protein 65
Protein pI : 4.14
Protein Weight : 19773.694
Protein Length : 271
Protein Note : chaperonin_like superfamily. Chaperonins are involved in productive folding of proteins. They share a common general morphology, a double toroid of 2 stacked rings, each composed of 7-9 subunits. There are 2 main chaperonin groups. The symmetry of type I...; cl02777

Protein Sequence : Show Sequence

>AAQ64514.1 heat shock protein 65, partial [Mycobacterium leprae]
EDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVKEGLRNVAAGANPLGLKRGIEKAVDKVTETLL
KDAKEVETKEQIAATAAISAGDQSIGDLIAEAMDKVGNEGVITVEESNTFGLQLELTEGMRFDKGYISGY
FVTDAERQEAVLEEPYILLVSSKVSTVKDLLPLXXXXXXXGKSLLIIAEDVEGEALSTLVV

Molecule Role : Protective antigen
Related Vaccine(s): Adjuvant arthritis DNA vaccine pCMV3.65 encoding HSP65 , Pristane-induced arthritis DNA vaccine DNA-hsp65 encoding Mycobacterium leprae 65-kDa heat shock protein

III. Vaccine Related Host Genes

1. Ifng (Interferon gamma)

Gene Name : Ifng (Interferon gamma)
Sequence Strain (Species/Organism) : Mouse
NCBI Gene ID : 15978
NCBI Protein GI : 33468859
Genbank Accession : NM_008337
Protein Accession : NP_032363.1
Other Database IDs : MGI:107656; UniProt: P01580
Taxonomy ID : 10090
Gene Strand (Orientation) : ?

DNA Sequence : Show Sequence

>gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
AGTATACCTTTGCAACC

Protein Sequence : Show Sequence

>gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
LLPESSLRKRKRSRC

Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
Additional Molecule Role : Vaximmutor
Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
Related Vaccine(s): Adjuvant arthritis DNA vaccine CII encoding type II collagen

2. IgG (partial)

Gene Name : IgG (partial)
Sequence Strain (Species/Organism) : Rattus norvegicus
NCBI Protein GI : 204718
Other Database IDs : CDD:209398
Taxonomy ID : 10116
Gene Strand (Orientation) : ?
Protein Name : immunoglobulin gamma-1 chain
Protein Length : 106
Protein Note : Immunoglobulin domain; cl11960

Protein Sequence : Show Sequence

>gi|204718|gb|AAA41373.1| immunoglobulin gamma-1 chain, partial [Rattus norvegicus]
RTQVPHVYTMSPTKEEMTQNEVSITCMVKGFYPPDIYVEWQMNGQPQENYKNTPPTMDTDGSYFLYSKLN
VKKEKWQQGNTFTCSVLHEGLHNHHTEKSLSHSPGK

Molecule Role : Vaximmutor
Related Vaccine(s): Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen

3. Il10 from Rattus norvegicus

Gene Name : Il10 from Rattus norvegicus
Sequence Strain (Species/Organism) : Rattus norvegicus
NCBI Gene ID : 25325
NCBI Protein GI : 148747382
Genbank Accession : AABR06074649
Protein Accession : NP_036986
Taxonomy ID : 10116
Chromosome No : 13
Gene Starting Position : 52824593
Gene Ending Position : 52828559
Gene Strand (Orientation) : +
Protein Name : interleukin 10
Protein pI : 8.68
Protein Weight : 19532.21
Protein Length : 178
Protein Note : Also known as IL10X

DNA Sequence : Show Sequence

>gi|389675116:52824593-52828559 Rattus norvegicus strain BN/SsNHsdMCW chromosome 13, Rnor_5.0
TCGGTATAAAAGGGGGACACCGGGCAGGAGATCTACACATTAGGGACTTGCTCTTATACTACCATAGCCA
CAACGCAGCCTTGCAGAAAACAGAGCTTCAGCATGCCTGGCTCAGCACTGCTATGTTGCCTGCTCTTACT
GGCTGGAGTGAAGACCAGCAAAGGCCATTCCATCCGGGGTGACAATAACTGCACCCACTTCCCAGTCAGC
CAGACCCACATGCTCCGAGAGCTGAGGGCTGCCTTCAGTCAAGTGAAGACTTTCTTTGTAAGTATAATGC
CCACCTAGCCTTTCTTCCTAGGATCAACTGAAATATGCATTCTGATGGAACTGTAGCTCTCCTCCTCCTC
CTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTTCTCCTCCTGCTACTCCTCC
TCTATCTCCAGCATCTATTACCCTTAAACTTGAATCCAGAGAGTCCTAGGCCAAGCCATGGGTTATGAGT
TAAACTAAATCCAGGCATAGCTGAAAACTAGGTTAACTAGCTAGCTAGCTAGCTAGCTAGCTAGGTTAAC
TCCTAGCTAGCTAGGAGGTGAATGCATTGACTCTCCCATGCTCAAGAACTTTCTGTTAAGTTTCCGATGA
GACCCCATGTTTTGCTGGCTAGGACAAAGCCTGGGGTAGAAGATACATGCTGTCTCTGCTTAGTCTCAAG
ATCTGGAGGACCAAAGTCTGGGGGTTTGGGGGAACACCAGAGCTCAGGAAACTGCTGTAGGAGGTCCAGA
GAAGGCAGAGAACCATGCAAAGCAGAGGCTTGCCGTAAGGGACTCCAGACAGGAAGGCTTGACACAGGTG
CCCTAGAAACCTCAACTGACTCATCTGAAAGAAGACCAGGCCTGGGTCAAACTTCTCCCAATCTGTTAAA
TTCAGTCAGAAGTTTTTAAAAACAACTTGATCCAAAGAGGAAGGACTTTGATTTAACTCCGGGCTCTAGC
ACCATCTAATCCTGTTTTTAAAACTCACCTTTCCAAAGTCACCAGGGCTACCTTGATTACAAATTCAAAA
CTCTACAAATGGAAAAGGTATTCAAATGGCACGCGTGATGGGTATTCCCCCAGCCCTTGTATTCATGGTC
CTATTGTCCCTCCAGCAAAAGAAGGACCAGCTGGACAACATACTGCTGACAGATTCCTTACTGCAGGACT
TTAAGGTAAGAATCCACTGGGTGCTGGGGAGCAATTAAGTGATTAGGGAGGGGAGGTGCTGCTTGTGACA
GAGAGAGCTGGGTCTGGTGCCGTGGCTTTCAAAAAGAGAAGTGAGAAGACCTTAACTCAGCATATTGCCA
GCAGTCGAGGGTTATGAAGAGCTCATTCTGTGGTAAAGAGAGAAAATGTAACTGGGGGGGGGGGGTCCCC
TAGGGACAAGACTGTCCATCAACATTAGTGGCAACAGTCCATCCTTCCAATAAGCCACACCCAACCTTTG
ATCCCTGCCTTTAGGGTTACTTGGGTTGCCAAGCCTTGTCAGAAATGATCAAGTTTTACCTGGTAGAAGT
GATGCCCCAGGCAGAGAACCATGGCCCAGAAATCAAGGAGCATTTGAATTCCCTGGGAGAGAAGCTGAAG
ACCCTCTGGATACAGCTGCGACGCTGTGTGAGTAGCAGACCAGTTCTTTCCCCTGTAGCCACCCAACAAA
CACTGTCTCCTACAATCCAGCCAGGCCATGCGCATCCAGACACACACAGACTAGACAGAGGACTAGGTAA
ATCTAGAGGGACACCCGGAGTCAGATCTCTTGCTTGTCTGTCTCTGGGCGAGAGTGGGGGTGGCTTTGAG
GCGATCACACGTGAAGATTTGCGTATAGCCTTCCTGTTATTTGTGAGTCATTGTGGGTCATTAGCTACTC
CCCTCTCTTCAAGGGAAGGCTAGGGCTTCAGTCATAGTTCCCTCGTGTTGGGGCTGCCAGCCAAGGCTCC
CCAAGCAGAGGAAAACATTGGTCTCTGTATCTCCTCTTTTGGAAAGCAAAGTCAGTGCCACACACCTGGG
CTGATAGAAATCTCCCCGGTATTTCAGAAGGGCCAGAAGACTACTGTCTCTAAGCCAGTATCTATGAATA
GCATATCCAGGACATCTATGTCTCTGACTGAAGTGATTCCTTCGCTGCTGTTGTTTCTTCTTTCTTCTCT
CACCGATACTGGAAAATCAGTTGGAGTCTACCTGTGCCCAACGATGGTTCTGGCCCAACAATTTGGCGCC
AGTAACTGAGGCTTTTTCCGATCCACTCTCAGACACCTAGAAATAGCTCATGGTGGTTGAGATTGGAGCT
AGACAAGGAGAGACATTAAAAACATAACATGTTTTGGGGCTGGCTCGGAGGAACAAAACCCAAGACCTAC
CTAAGTTCACACCCACGCTCAGCAAACCATGATTTCAAAGACATACATTGTATCTTCTTTAGGCTGTTTT
CCTCGGCACAGAAAGATAAAGGGCCAATCATGGCTCAGTTCTCCCTGGGGGCTAGGAAGTAGATATCGAA
GATAAGCAAGGGTAGCTCCTTCCTCATTACAAGAAGATAGTTTCCTCCCAAGAGCCATGGATACAGGGTT
CGAGCAAGAATTATCAACACTCTTTTTTTTTTTTTCTTCTACAGCATCGATTTCTCCCCTGTGAGAATAA
AAGCAAGGCAGTGGAGCAGGTGAAGAATGATTTTAATAAGGTAAGCCTGGGCCATGGCAAAGAGAACGCG
TGGAACGTGACCTCCGTCCGTCCACTCGCAAGGGCAGAAAGAGGGCCCGACCTATTCCGACCGAACCTGG
AGTGTGGGAACCAGCAAATGGTGTGACCTCCTCTGCCAGTTAGAAAGCCACCACCTCAGTCACATTTGTT
TTCTGCAAAGCGTCTCTGGCAGTTTCTAAATGACTGCTCCACTTTTGCAGGCTTTTGGCTTAGACTGACC
AGACAGCCTATAAGCGCAGAGCGCTAGGTGCTGGGGAGAGCGCCGTAGGAAACAGAAAGTACAGAAAGTA
CCTTGTTGGGAAACAGGCAGAACCCACAAGTACAGAAAGCAGACATGCATGAGTGCACGCAGGTACATCT
TGGGGCTCAGCCTGGATGCTCCTCGGAACCAGAAGGAGCAGGAAGCAGAATTCTTAGGGGTCCCAGGGCA
CTCTCCACCTGGCAAACAAAATGAGGTTTCATACTTCCTTCTGCCTGTGAACCTATTCAACCCTAGTTCC
CAGAAGCCATGTGGCCCACATCTTCTTTGTGGGCTAGGCAGAGCTCCAACATCAGTGGGCATGGATTCCA
TGACAGCAGGCCAGAGCAGCGGGGTGAAGACTGCCTACACACGCAAAACATTAAAAAAAAAATGTTTTTC
CTTGTTATCTCTATCACTGTCCTGCTTTGAAATTGCTTCCTCTAGCATCTTTGCATAACATTCTGCATAC
GCATTATGTGGGCACTGGCCTCTGGGGGGGGGGGGGGGAGTTGAAAAGGCTCTACTCTGAAGACAGTGCT
TTGAGAACCGAATGCTTCCCTTTCCCTACTGACCCCTGGCAGTGCTTAAAGTCGGAGAAGGTGCAAGGGA
AGACACAGCCCCTTCCCAAAGCAGATGAACAAATCTGCTTTGGGTCCTTCAGCTCCCAACACAAGAAAGC
TGAGATCTTTCTACACTCAGTGGCCTTCCTAGTGCAGGGAGCCCCTCAGCCCTCCAATGGGTCCTAACCA
GATTCTTCTCTCCCCACACAGCTCCAAGACAAAGGTGTCTACAAGGCCATGAATGAGTTTGACATCTTCA
TCAACTGCATAGAAGCCTACGTGACACTCAAAATGAAAAATTGAACCACCCGGCATCTACTGGACTGCAG
GACATAAATAGAGCTTCTAAATCTGATCCAGAGATCTTAGCTAACGGGAGCAACTCCTTGGAAAACCTCG
TTTGTACCTCTCTCCAAAATATTTATTACCTCTGATACCTCAGTTCC

Protein Sequence : Show Sequence

>gi|148747382|ref|NP_036986.2| interleukin-10 precursor [Rattus norvegicus]
MPGSALLCCLLLLAGVKTSKGHSIRGDNNCTHFPVSQTHMLRELRAAFSQVKTFFQKKDQLDNILLTDSL
LQDFKGYLGCQALSEMIKFYLVEVMPQAENHGPEIKEHLNSLGEKLKTLWIQLRRCHRFLPCENKSKAVE
QVKNDFNKLQDKGVYKAMNEFDIFINCIEAYVTLKMKN

Molecule Role : Other
Related Vaccine(s): Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen

4. TGF-B from Rattus norvegicus

Gene Name : TGF-B from Rattus norvegicus
Sequence Strain (Species/Organism) : Rattus norvegicus
NCBI Gene ID : 59086
NCBI Protein GI : 11024652
Genbank Accession : AABR06003977
Protein Accession : NP_067589
Taxonomy ID : 10116
Chromosome No : 1
Gene Starting Position : 83742150
Gene Ending Position : 83758471
Gene Strand (Orientation) : +
Protein Name : transforming growth factor, beta 1
Protein pI : 8.63
Protein Weight : 42045.82
Protein Length : 390
Protein Note : Also known as Tgfb

DNA Sequence : Show Sequence

>gi|389675128:83742150-83758471 Rattus norvegicus strain BN/SsNHsdMCW chromosome 1, Rnor_5.0
TCAACCTCAAATTATTCAGGACTATCACCTACCTTTCCTTGGGAGACCCCACCCCACCCCACAAGCCCTG
CAGGGGCGGGGCCTCCGCATCCCACCTTTGCCCGGGGTTCGCGCTCTCCGAAGTTCCGTGGGGCGCCGCC
TCCCCCATGCCGCCCTCGGGGCTGCGGCTGCTGCCGCTTCTGCTCCCACTCCCGTGGCTTCTAGTGCTGA
CGCCCGGGAGGCCAGCCGCGGGACTCTCCACCTGCAAGACCATCGACATGGAGCTGGTGAAACGGAAGCG
CATCGAAGCCATCCGTGGCCAGATCCTGTCCAAACTAAGGCTCGCCAGTCCCCCGAGCCAGGGGGAGGTA
CCGCCGGGCCCGCTGCCCGAGGCGGTGCTCGCTTTGTACAACAGCACCCGCGACCGGGTGGCAGGCGAGA
GCGCTGACCCGGAGCCCGAGCCCGAGGCGGACTACTACGCCAAAGAAGTCACCCGCGTGCTAATGGTGGA
CCGCAACAACGGTGAGCTCTGAGGGGCTGGGGAGCCAGGAGGGAGCCCCCAGGGGGCGCCGGAGTGCAGG
GGTCACCGGGAGGAAATTACCCGCAGAGGAAACTGGCTGGAGGAAGGGGACCCCTGGGGGCGCTGGGATG
ACTGTTTGGGTGTCCCAAAGAGGATGGCGCTTACCCCTACCCCAGGTGTCTGGTCTTAGAGAGAAAGACA
GCCTGCAAAAGTGAACCTCCCCTAGAAAGGCATGATCCTGGGGAGAGTCCACTCTAGAAGAGGAGCATTT
GTCAGGTTTTGTTTTAAGTTCCCCTTATGGTGCCTGAAAATGCAAGGCTCGTTGGGTCCTTTTCCAGAAA
GGTAAAGCCCGGCTCAGGAAAAATGGAAGGCGCCCAGCATTTGGGAAACAAGAGACAGTGTGGAAAGCCG
ACACTAGACCGGGTCGTGAGATGGAGAGAAGAGATTCAAGGGAAAAAAAAAACCTAGGGGATGGGTACCC
TAGAAATGCCAAATAAAATCTCTACTGGGCTTACTACCAGAAGGCGACAGGCGCGTGCTGGAGGGTGTGA
TCTGTGGAGTCATTGTGGGGTTCCAAGTGATAGAGGAAAGCGAAGTTGTTAGGAGAAGGAGGCTAGAGAG
AGAGGAGCGGAGAGGGTGCGGAAAATGAGCCCGGAGGTGTCACGAGTGTTTTGCTTGGGGGCGTAAAAAA
GCTTGCGAGCGGGCAGGCGGAGCCCAGTGGAGGAAGCGAGCGAGTCCTCAAGAAATTTAGAATACTGGGT
CATTGGGAAGGAATTATAAAAGAGGGGAGACTGGCGTAGGAAGGTGGCCCCGCGAGGCGGGCAGGCAGAA
CAGGGATACTATGCCATCCAGGCGAGAGTGGCGAGCGCCCCTCGCCTGGGCGTGGAAGGGAGTGGCTGAA
CCTCGGGCCCTAGGGGAGCTGCTAGAGGTAGGAGAGAGGCAGTCTTCAAAACCCAAATGAAAAAAACGAG
TCGGTAAGGGTGAGGAATCTCCACGTGGGTGCCACGCGCGTGGGGAGGAGCTTGCGCTTCTTGAGGGGGT
TAGAGGACCCCACGGAGCTGAACGCTGGCAGGCTGTACCCCCAAAGTCCCAGTTCCTTCTACGGGAGGCT
GGGGAAAACCCAGCGTCCGGTCGCCTCGCCCTCCTCCCTTCCCTTTCCTTTCCGTGCCCAGGAGGAGGGG
GGAGACTGAGAACGCGCGCGCGGAGCCTGGGGCGAGACGAGGCAGGTCCGGATCCCGCCCTCCCTAGGCT
GTTAGTCTCCCGCCCTCCTCACCCCAGCAGTGGGCGGGGACACTCACGGCCTCGCCCTTTAGTCACCCAC
GCGGGACCTAGGAGTCCAGTGTGACCGGGAACTGTGGGTGTCAGAAGCTTCTCCAGGTTTCACCGTCCTA
ACCCCCAGAGGTGGACTTAAGAATTCCTTGCACATCGCTGAACCCAATTGTGGCTCAGGCCTAATGACAC
CCCTCCCTAGTCACCATTTAATGTGACCTCTTCGTTAAAGTCTTTTCCTCTCCCCTAAGACTGTATTTCT
TCTCTTACGCATATATCTCTTCCGGCATGTGCCTCCCCGTTTCCGTCTCCTCTCCCACGTATCTATTCAT
CTCTATGTCACCCCACTTCCCTCTGCGACACCCACAATCCTTCCTGCCCTCTTATCTCTCCCCTATCTGC
TACCCCCAACTGAGATCCCACCCCCTCCATTGTGGTTTGCTGAGTAACAACTGGTCCAAGAATAGGGCCC
CTCTGTCCTTCATCGATGTCTGGGTCACCATCTCATCCCCACTACAGGAAGTCAAAAGCTCCAGTTGATG
GAGGATTTCAGGACTTTGCCCCTTCGTCCACCGACGCAGACATCCTTTAGCCAAGGAAGCCCTTTAGTGA
ATGGGGCTCCCAAACATGCAGTTATTCTTGCCTAGAAATCAACCTCGCTACTAACCCGGCCAGGAAGTGG
GCTAAAGAGGGAAGTAGCCGATGATAGGCAGATGATAATTACCCTGCACTGGCCAGGAAGTAACAGAGTT
TCACGATTAAGACCACAGTCTGGTTGTCTCTACACCTGATTCCCCACCCCCCCAACTCCCCTCTCCCACT
CAGTTCTCTCCCTTCATCACCTCTGATGGCTTCCTGACTTGATGTGCAGGGCTGGATTGGTACCCAGCGG
TGAGGGAAGGGGCTGTGTGAGGGGGGGACTGGAAAGAGGGAAATCTTCCGTTGGGATGAATAAATAAATA
AGTAAATAAATAAGTGGGGGGGGGGACAAACCACCATCTGCCAAGCAGAGCCGTCCATGACTGTAGTCCC
AGTACGAGGGAGATAGAGGCGAAAGGATTGCTTCGAGCCTAAGCTATGAGATCCTGTCACCAGAAACAAA
AGAAAATAAACAAACAAAAAATTAGCCCCTAGCATAAGATGGCTTGAATCTTACCACATTGTGAAGTCTT
GATGTCTTTATCTTTGTAGTGTTTAAAACAATTTCGTTACCATTCACTGTATTCCCCTCACCCTTATTCA
CACATGCATGCACGCATGCACGCAGGCAGGCAGGCAGGCTAACTCCCAGTGAACAGCTTCCTGACTCGGG
CTCCTGAGTGCTGCTGGCTGCAGACTCGCACCGGCTAAAATACACATTATCGACGTGTGTGTCACATGTT
TAGCTTTTGCCCCATGTCTTATCAGCTCCCTGGAATTGGATGGTATCTTGCAGTTAAAGAGAAGAATTTG
CTCACCATTGGGTACATAGTGCTTCAGTTGAGGCCACCTCAGTTGGGAGTCAGAACTTAATGGTACCTGT
TTCTTCCAAGGTCAGAAGGAGATTGGCCCAATGGGAGAGCATTTCCCTAGAATCCACCTTTGAGGTGTTC
GCTCCAGCACAGCTGTTCGTTCCAGCCTAGAACCCGCTGTGGAAGTCACTCAGTGCCACTTTCTGGTCCC
CACAATTATGCTATGCCATTCACTCTACAACTGCTGTGTGGTTTTGTTTGAGGTGTGGTTTTGTTTGAGG
CGTGGTCTCGCAAAGCCCAGGTGAGGTCAGCCAGTTAAGAGCATTAGAGAGGGGCTCAGCAGTTCAGAGA
ACCAGCTGTTCTTCCAGAGGTCAGCCGGGGCCCACATGGCTGCTCACAGCCCTCTGTGGCTCCAGTTCTA
GAGGATCTGACGCCCTCTTCTGGCCTCCACAGGCGCCAGGCATACACTCAGTACACAGACGCACAGACAC
AGCCGAGAACACCCATAGACTTAAAGTTAGAAGAGTGGACATGTCAAAGTGGAGCGCCAACCTGAGTTAG
AGCCCATGGGCTCAGAAGCAGGGCACCTCTTTTTCTCCCATCCTCGCCACAGACCTGATGTCTTTCTCCT
TCTGCTCCTCCCCACCCAGCAATCTATGACAAAACCAAAGACATCACACACAGTATATATATGTTCTTCA
ATACGTCAGACATTCGGGAAGCAGTGCCAGAACCCCCATTGCTGTCCCGTGCAGAGCTGCGCCTGCAGAG
ATTCAAGTCAACTGTGGAGCAACACGTAGAACTCTACCAGGTGAGGCTTTGAGCCTGAGAGGTGGCTCAC
AGGTCACAACTGCTGAGGTGTGGCCAGGTGGCTCTGTTTGACAACTACAGGGCACACAGCCAGTCCTTGA
GGAGAGAATACAGAGTGTTGAAGCCTAAGGGTCTTCTTGTTTTCTTTTGGTTGGTTGTTTCGGTTTTTCA
GGACAAGGTCTCTGTGTAGCCCTGGCTGCCCTAGAACTCACTCTGTAGATCAGGCTGGCCTCAAATTCAC
AGAGATCCATCCACCTGCCTTTGCCTCTCCAGTGCTGGGACTGAAGGCAGGTGTGTGTCACCATAGCCCC
CGAGAATCCTAAGGTTTTTAAAAAATGCTTAGATTTCGTCCCATTAGTTGAATTTGGGATGTTGAACTAA
AAATGCAGAAAGAGCCATGGGATGGATATCTGGATTTGTATGCTTGCCTAGAATCCCCCAGTGAGGGGCT
AGGGGCGTGGCTCAGTGGTAGACTGCCTAGCATGAGTAAAGCATGGGGGTGGGAGTGGGTGGGTGAGGAG
AGGGGATAACAGTGAGGAAGGAAAGGAAGTTGGCAGACACTTGCAATCCCAGCACTTGGAGGATGGAGGC
AGGAGACCAGGAGTCTGAGGCCAGCCTGAGCTACTTGAGACCCCGTCTGGAGGCTGAGGCAATAAGATCT
CTTGAACCCAAGTATTCAAGTCCAACATGGAATAGTGAGATCTTGTCCCGGACAGAGAAATTAAGCATTT
GAAATGCCCTTGTAGTCTCTTAAGTCAGCAATCACCATTCTGGACTTCAAAAGTAGCTTCTTTTTTTCTT
TTTAAAGACAGGGGTTCTCTGGGTGTCTTGGAACTCAGCAATCCACTCACCTCTGCCTTCTGAGACTTGG
GCCTTAAAGGCTTGGGCCACCACTGCCAAGCCAAAAATAAAAATTTTAAACCAGGAGGCAGAGGCAGGAG
GGTCTCAGAGTTCAAGGCCAGCCTGGTCTCCATTGTTAGCTCCAGAACAGCCAGGGCAACACAGAGAGAC
CCTGTCTCAAAAAACAACAATGACAAAAACAAAAACATTGGGAAGACTGTTTTGTGAGTGTACTCATAAT
AATAATTGTAAGTAAGATAACAAACTAAAGATAGAGAGATAGATAGATAGATAGATAGATAGATAGATAG
ATAGGAAAGAGAGTCACCACTCTCCCAAACTCAGGATAGTAAACCGGTTAAACTGTGCTCCACAGTATGA
GCAATGAGCACTTGCGTTGAGGGCCCTCCCAGCTGCTCAGCAGCGTCCACCATGGAAGAGATTGAGGTCC
CGCAAGGCTGTTGAGTGCCCATAGCTTGCACAGAATCTCATGCCCAAGGGGGAGCCTCACGACTGACCAC
AGCCTGGGGTTGTCGTTCCTGTCCCATGCTGTGTTCCAGTCTCTGTCCACCCCTCCACCCCCACTCCTGG
CTACTTTTTGCTCTTGGGTGCTGTATGTGAGGGCGTGGCCTGCAGGATCCAGAGTCTGTACGTGTCAGAA
GCCAGTGTCTGGGGGATGCCAGTTTGTGGGACAGGCAGCATGGGTTTGCAGCCAGATGATCTGGGGACAA
GATGGCAGCCACCATACTCCTCTTTGCTTTCTTTGTTTCCATTCAGAAATATAGCAACAATTCCTGGCGT
TACCTTGGTAACCGGCTGCTGACCCCCACTGATACGCCTGAGTGGCTGTCTTTTGACGTCACTGGAGTTG
TCCGGCAGTGGCTGAACCAAGGAGGTGAGATTGCTTGTCTTGTACCATCCTCCAGTGGGCCCCCCCCCGT
TTGTCCTGGCATCATTGTGCATAGCACTGCATTCCCCCCAACTGCACCCCTAACACACACACACACACAC
ACACACAAACACACACCACACACACACTCACACACACACACACACACACACACACACACCACACACACAC
ACACACACACACATACCTGGTACTGATGTCAAGAAGTAGTACTTCACAGTTCTTACCAATGGATCAATCT
GTGGAGGGATGCTAGGGATAGACGGTGACCCAAACAGTCCCTATCCTTGCCTTCAGTAGACCGAGTGGTA
CCTTGGACAGTTGAAGGATAGGTTGACTTTTAGCTGTGCTTTATGACTCATCGTAGAGAGGAACGTGGTT
GAGCACTCAGGAGGTGCCTGACTGTCTGGATTCAAGGAGGGCTTCCTGTAGGAGAGGGCATCTGAGGTAA
TGTCTCTGAGATGAATAAAAGCAAGCAGGAAAAGGAGAAATGAGAGTATCCTGTACCAAGGCTCTGCAGC
CAGGGAGAGAGCCTGCTGTGTCCGTTTGTCCAGGAGTGAACAGTCTCCCTTGTCTCTTGTGGGTCCTTCC
CTTGCAAGGCCCACACGATCTTTCCAGACACTGGTAAGGTCCTGTCAGAGCCCTAGCAGGCAGTAATGGC
CTATAAGGATGTAGGCCAGAAAGGGAGACCTACAGAGAGGGCAAGGCTTAGATGAGGGAAACCAAAGTGT
TGTGGAAACCCAGGCAAGGTTAGGACCCAGGAGGGCTTCTGGGATGAGAGAAGACTGACTCATGGGATGT
GTAGTTTAGCCACATGGAGGATGAGTCCGAGTGGGCTGGCACCAAGAGGAATGTGGAAAGCCCACTGGAT
GGAGGTCAAGAAGAATTTTGTGTGGTATACAGTAGGCGCTTAGTATCATTTATGTGTCCACTCAAGGAAC
ATCTCCCTTGAACCAGTAAGATGGCTCAGCAGATAAAGGTGCCCACTGCTGAGATTAATGGCTTAGGTTC
AAGCCCCAGGAACACCCAGGAAGTTGGAGGGAACCAACTTCTACAAATTGACCCCTGACCTCCCTGCATA
CCCTGGCACATGTGCACCCCCCTGAATAATGAGAAGAGGCCAGCTTAGGTTTGCACCGTGCCTTTGCCTC
TGGCTCCCTGTGTGGCCCTGGCTAAGTCGCTTCCTCCTGGAGTGAAGGACTCTGAAGATCAGTGGAGTTT
AGATCAGTGAGTGACTGGCGTCTGTGTTCGCTCTGAATGTCCTTCGACACCAACACTGCTCCTGGCCCTT
GGCACCTAGATCATGATGATGTTTATCTTTGTGACCAAGGACCAGGCTTTACAGAGCAAGAAGATAGCAA
GAACCCAGGGCAACATGCCTGATGGTACGAAAGCCTGCTAAACTCTGCGCTTGGGAGGCGAAGGCAAGAG
GATCATGAGTTCAAGGTCTTAGCTCCATAGTGAGTTCGAGAATTTGAGGCCAGCTTGGGCTACGTGAGAC
CCTGTCTCCAAAGAAAAAGAGGGCCATGGAGAGGGGTCATGGGCGCAAGAGCTTCCATTTTGCAAGCACA
AGGACCTGAATTCTCATCCCTGGCACTCATGTAAAAGTCCGACATTGGCGTAGTGTGTGCCTGTAACCCC
AGCCTTGTTGGGGGTTCGGTGTATGTATGGAGACAGGCCAACTCATGACTCTGTGATCTGCCAATCTGGC
CAAAGGGGTGGGCTCAAGTCCAGAGATCTGTCTGAGGCAATGAGGGAGAAAGGATTAGAGGAAGATGCTT
GTCACCCAGGTATAAGCCTACCTGGTATAAAATGTACATTATACATGCATACATATACATATATACACAT
ACATATCTACAGTTCCAAAAAATATAAAATGAAAGGAAATAAGGCAGGCACAGGATCCCTCCTCTGTTCA
TGTTGTTCTACTGAGAATAATCAAAGAAGTAAATACTCATGCAGAAATGTCAGAGCCCCTGCCCGCTATG
GAGGAAAGGAATGGATGCCACATTTGGAGAGGGAAGTGTCTCCTCAGAGCCTTTCCCAAGGGGTTGACGT
TTGAGCTGAGCCCTGAATAAAGGGGAGAAGGAACCAGCAACTTAAAGAGCGAAAAGTGTGGGGTGACTAG
CAAGGAGGCCCCAGGGCTGGCAGCTTCCTGCCCCCTCTCCCTTCCCCCCATCAAGGCCTCACCAGTCACC
TCAGACTCCTTTCCTCTCCTGCAGACGGAATACAGGGCTTTCGCTTCAGTGCTCACTGCTCTTGTGACAG
CAAAGATAATGTACTCCACGTGGAAATCAATGGTGAGTTAACTTCCCAGCTCCTGCCGGACACAGACCCT
GCATGTGCGTGCGTGCGTGCGTGCGTGCGTGCGTGCGTGTGTGTGTGTGTGTGTGAGAGAAATCTCACTC
GCCCCTTGCTCACCCATCCATCCCTCCACAACATTCAAACCCAAACAGGGATCAGTCCCAAACGTCGAGG
TGACCTGGGCACCATCCATGACATGAACCGACCCTTCCTGCTCCTCATGGCCACCCCCCTGGAAAGGGCT
CAACACCTGCACAGCTCCAGGCACCGGAGAGCCCTGGATACCAACTACTGCTTCAGGTAAACTCTGTCTC
TCTCGGAGGCTGCACAGGCTGGGGGCTTGACTGCAGCCTGTGGACTGGTGTGCCACCATTCATGCCTGGA
GGGCTTGGGGACAAGTTCTTGGTTCTGTGGGCAGCTGAGAGTCTAGAATGTGGGGTGTTTTCTGGCTGGA
GTGTTCAGGTTAGCACAAGAGTTAGAAGTCGGTTGGAAGGTTATCTTGTTGGGTTTTAAAATTCTTGCTA
GGCTTCCACCACTTTGCAATGCTAATGAAAGAATTTACTGTGAGCAATTGAGAGTGCTGACCCTAGAAAG
CGGGAGAGTTGAGATCCAAGCATTTGGGTTCAGAATCAGAAGGCGGTCTGCACTGGGTTCCCACACTCGA
GGCAGATGGTGGTCTCCAATGCTGGTAGTTGGGAACGACAATTATTGGGGCTCAGAATGGCAGGGTCTGG
CATGGAGATGTTAGCGTTCAGAGTGGAGGTGGAGAAAGCCAGAATGTGAGTCCCCTGAGGGTCAGGGCTT
CATCTTGCAGTGGTGCACCCTGAGGAAGCTGGGAGGCGGAACTTCCTGAGATGTAGGCTTACAAGTGTGG
AGTGTTGGAAGACAGCACTGAAGCAGGGAACTCTGAGGAGTCGGAGAGTGGGCATCCTAGAGTGTCCAGA
ATGGCCATCCCAAGGGCAAATGCTTTTTGGTCATTGTTTTTAAATTATATCTGTGTGTGCGTGTGTGTGT
GCGTGCTTGCGTGTAGGGGCACCCATGCCATGACACTTGTGTGAAGGTCAGAGGACAACTGTTGGGAGTC
CGTTCTCCTTCCACCGCGAGTGTCCCAGAGATAAGACTCAGGTTATCAGGTTTGGCAGTGAGCACCTTGA
CCCACTTTGTTTTGTTCAAGACAGGGCTTTGCTGTGTGACTGAGGCTACCCTCAGATTCACTCCTTTAGC
CCAGGCTAGCTTGAATTCTCTGTCCTGGGATTACAGGTGTTACCCAACCACCCCATTTCAGACAGAATGG
CAGACTCATACTAAGCCTCGGTAATGCGGATTGCGAGAAAGAAGTTGAAACACTAGATCCTAGGAGGTGT
GAAATTCAGGAGAGTTGAGATGAGAGCAGTGAGCTACCTGTCCCTTCCCTGCTTCAGACTTTATAGAGTG
CTGTGTTGTATGTCGGTCCTTGGCTGGAGCTGCTCCTCCCCCTCCCCCACCTCCACCTCCTCCTCCTCCT
CCTCCTTCACCGTCCCTTTTTTTTTTTTTTTTTTTGGTTCTTTTTTTCGGAGCTGGGGACCGAATCCAGG
GCCTTGTGCTTCATAGGCAAGCGCTCTACCACTGAGCTAAATCCCCAACCCCCCCAACGCCTTCACCGTC
CTTTTGCTGTTAGCCTTCACATAGGAACAGATGGAGGGGCTTCACACTGCAGGTTACTAAGAGATGAGAA
AACTATGCAAGCTCTGTTCCCCGCATACTGGGAAACGAACTTAGGGAATCAGGAAAATCTCTTTGTCTGT
CTGTCTGTCTGTCTGTCTGTCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTCTCTCTCTACATTTGTTTTG
TTTATGCCTGATGTATGTGTGGGCTCTGGGAATCCAAACCCAGGTCTTCATGCTTGCATAGCAAGCACTT
GACTCACTGAGCCACGTCCTCTGCTGTGACAAGTCCCCAGTTGCTTGGAAGAACATAGTGCCAACGAAGA
AGGCCTGTCAGATACATCAACCGTGATCTGATGGGGATGGTAGCTCCAACTCCCATGTTTCCCTGCAACA
GAGGGACAGACTAGCAACATTAACCAGCACCACAGGGATGCACCCAGCAAAGTGCAGATGGTGGGAAACC
TAGTCGTTTCTTTAACACATACAGTACAAAGAAGGGAGGGCAGGATATATAACCCACAGGACTAGAAGAG
AGGGAGAGAAGGGCCAAGAGGTGGGAAGAGGAGGAAAGAAAAGGAAAGGGAAAAAGACTTTTATGGGATA
TGGTGGGAAATATGAACCTTGAGTAGTATTTGATTTTAGTGAGTTACTGTTAAATTCTTGACCAGGGTTG
GGGATGCTGTGAAGAGATGGCTCAGTGGTTAAGAGCACTGGCTGCTCTTCAAGAGAACCTGGGTTCAATT
CCCAGCCCCCACATGGAGGATCACACCTGTGTGTAACTCTTGTTCCAGAAGATCTGATGCCCTCACACAG
GCATAGATGCAGGCAGAACAGCAATGTACAGAAAAAAATTCTTGCCTAGGATTTTGTTGTTGCTGCTGCC
TTTGTGATACTGAGGAGGGAACCCAGTACCTTGTACGTGGTAGAAAAGCAGTTGACCACTGAGCCACGCC
CCCAGCCCCTCACTGGGGGATTCTAGGCAGGGGCTCTACCACTGAGCCACGCCCCCGGGCTCTACCACTG
AACATATTGATCTATACTTTTGGTTCATGTTTAAATTGTAAAATATTCTTCACTTTCTAAGAGAAAGTGA
ACCATTCTATAAAAGTTCATTAAACAAATGAGAAAAAAACTACAGTGGCGGACAGGAAGTGTAGGTAGGA
TGAAATTAGCCACGAGTCAGCAACTGTTGCACTTAACAATTGTGGGGATGTGAATTAATTAAGAAACCAT
AATTCAGAAGTCCAAAAATCTCTCTGGCCATGATGAGGAACAGGAACAGAAGGAGCTCAAAGTCATTGGT
AGGTATGCAGTGTGTTTGTGGCAACCTGGACTACATTAGACCCTGCCTCAAGAAAAGGGTTGGGGCACCA
GAGCTCAGCTGCTCTTTTATACAGGACGCAGCAGCTCTGGGGCGTTCTAGGCACCCACATGGCTGCTCAC
AACAATCCATTAAGGCCAGTTCCAGGGAATCTCATTCCTTCTTCTGGCCTCTGCGGGCACTGCACGGATA
TGGCGTGTGCACACATATGTGCAGAGACATACTCCTACACATAAAACTTTCTTTTAAATTGCCAGGTGCG
GTGGCGCATGTCTCTAATCCCAGCATTCAGGAAGCAGAGAGAGGCAGATCTCTGTTTGAGTTTGAGGCTG
GCAAGTTCACTCACATAGCAAATTCCAGGACATAACATGTCGAGATCTTGTCTCAAGAACTAGGAGGAAT
AATAAAAATAAAATTGGGGGCTGGGGATTTAGCTCAGTGGTAGAGCGCTTACCTAGGAAGCGCAAGGCCC
TGGGTTCGGTCCCCAGCTCCGAAAAAAAAAAAACCAAAAAAAAAACAAAAAAAAAATGTGGAGCAGGAGG
GTACTTGACATTTTATAAAGTCTGTGTAAATATCCCCTTATCCCCAAAAGCTTTTAGTCCCTAGCACTTC
TGAAAAGGGGTATTCAGTTTGTATTGTAACACTCTGCTTCTGTGTCCGTTCGCAACTTTCCACGGCAAAG
CTGGTTTTATGCCAGCTTATCGGCTCAAAAACCACCTCCCCCAGGGCCGCAGAGAGGGCCCTTCACAGAT
TAGGCACGGCTGGCTCTCAGTAGACTGGCAAGGGAGGCTTGAAAAAGCAGTGGCTGTGAAAATCCTAAGA
CTGCTTCCCAGAAAGAAAGGCCGATATGTGCCAGGCTGTGGTGGTGCACAGCTTTAATCCCAGCACTCAG
TAGGCAGAGGCAGGTGGATCTCTGTGAGGGCAAGTCCAGCCTGGCCTGTAGATTGAGTTTCAGGACAGCT
GGGGTTACAGAGAAGAACCTTGTCTCCAAAAAAAGAAAGCAAGCGAGCCCTGGGGTGTGTCACCCTGGGG
TGTGTCACCCTGGGGTATATCACGGTTACCCGGAAGGCTTGCTTGTTCAATATGGGCCCAGTGTCTCTGT
CTCAGCAGAAACGAGTGGGTAGAGTCCACAGGAGCCCCAGTACCCTGAGCCTGGAGCTCTGTGCTCTGAA
AAGGAGGTGTCACCCTGTCCCGGGAGCTGCTCACGAGAACCTCCCTTTCAGCCACCCTACCCTGTTGTGT
CTCCTTCCAGAGTTTCTTTCTCTCTGTGGCATACCTTCAGATGCTTTGTATGTCATTGTTGAAAACAGGG
TCTCCCCGTGTAGCCCTGCCTGGATTAAAATGTTCTGCATGGACCAGGCTGGCTTTGAACTTGCAGCGAC
CCTCCCATTTCTGCTTCCTGACTGCTGGGGTTATAGGTGTGCACGTGTGCATAATCATGCCTGCCTGGCT
CACATGTGTCAAATTTTATTTATTATTTTATTTTACATGCATGTTTTGCCCACATGGGTATATGTGCACT
GTGCATACGTGATACCCAGAGGACAAAAGAGGACATTGGATCCCCTAGGTCTGGAGTTACAGATGGCCAT
GAGCCTCCATGGGGGTGCTGGGAATTGAACCTGGGTCTTCTGCAAGAGCAGCCACTGCACTTGACCACTG
AGCCATCTCCTAGTCCCTTGCCCACATTTTTAGAGTTATTTTTAAAGGTGTAATGAGGAGGAACCTGCAT
TTTCCTTCCTTGAATTACTTCGTTCTGATTCTAAAAATAATCCCAGCCCCTGCTAAGAGTGTGGGAAATC
CAGAAAGCCATCACACCAGGGAACATAAAGACCCACGGGCAGCATCTTTAGTGCGCAGTCTGCCGGCCTC
TTTGTAATGGGATTTGATTTACTCACTACCTGCATCTTTAGATCCTGATTCTGATACATGCCGTGAACAC
AGGTGCCCAAGTGTTCTTCTGGTCAGCCTGGGCTACAAGGTGAATTCTGGGGCAACTTAGGGTACATGGC
GAGACCTCATCTTAACTTTCCTCCCTCTTGAGGGGATCGTGCAGGGTGAGGTGGCAATTGCTGGTAATTC
CAGCACTTGGGAGGCGAAGGCAGGAATTATCAGGAGTTCATGGCCAGCCTGGGTTACCTAACAAAACCCA
GTCTTGAAACAAAGGACTGACCAGTACTCAGGAAGCAGGGACAGGAGGGCCTCGGGCAGCCTGGTCTTTG
TAGTGAATACTGGGACAGCCAGGACTCCACAGAGAGAGTCTCTCTTAAAAAAAAAAAATGTAATGTTTGT
CAGTTAATTCATATAACCAGCATTTGCCAAGCATGTGTACATTAAGAGACACTGCCCCATGGTGAGATGA
ATCAGATGATATTTAATAAAAACAAGCGGAAACATTCTGAGCAGGAAAATAAAGCTGGTGAAGGGGGCAG
GTTAGAAGCCAGGCCAGGAAAAATGAATAATAGTGGAAGCCAAGTGTGGTGCACCCTTGTGATCCCAGCA
CTTGAGAGGCAGAGGCAGGAGGGTCAGGAGTTTAGGGTTGGCCTTGGCTACAAAGGGTCTTGGAGTCCAG
CCTTGGCTACATGAAAGCCTGTTTCAGAAAAGACAAAGAGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC
CTTGCCTAGGAAGCGCAAGGCCCTGGGTTCAGTCCCCAGCTCTGAGAAAAAAAAAAAAAAGAACCAGAAA
AGACAAAGAACAAAAGACATCTTGGGGACTGATTAATTTTTCTATTCTGATGAAAATCATTTTATGGTGG
AAAATTTAAATATAGACAGAAAAAGCAGAGGTAGTATAACCTTCCAGCTTCCAGAATTATCAGGATTTTG
ATGACTCGCTTCAGACAGTTTGGGGCTGGAGACTTGGCCCAAGAGTTAAAAGCACTGGCTGTTCTTCCAG
AGGACCTGGGTTCAGTTCCAAGCACCCGTATATGGTCGCTTAAAAGGACAACTATCAATAACTCCAGTTT
CCGGGAACCTGATGCCCTCTTCTGGCCTTCTTGGGCATTGCACAAATATGGTGCACATAAAAATAAATCT
TTAAAACAGAACAAGCCAGGTGGTGGTGGTGCACACCTTTAGTCCCAGCACTCTGGAGGCAGAGACAGGT
GCGTTCCCAAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCTGGGTCAGCCAGGGCTACACAGAGAAA
CTCTGTCTCAAGCAAGCAAACAAACAATACCGAAACAGCTGCATAGAAATTCACAGTCCTTGCAGTAAGT
GTAGCATTGAATTCCCAGGATGGAATTGAACAGTTGTATGGAGGGACGCTTTGGATGGCAAGTGGGCAGG
GCTGGTCAGAGGAGGGAGGCACTGTTCATTTATGTAACGGGGATACTGAATATCTGCCTCAAGCCAGGCA
GCATTTTTAGGAGTTAAAGCTCCCTGGGTGGTAGATAGTATTTAAAGGCACCGTCTTACCCTGTAGCCGT
AGCTACCCTGGAACCGTCAGGGATCCAGCCTACCCTGCCTCTTTGTTGGTAGTAAAGGTATGCACCGCCA
TGCACGGATTCACTTTAATCTTGTTGTTATTTTGTGCAAGTGCCCACACATGCTGTGGTGCACACGAGCA
GGGCAGAGGTCAGTCTCGTGGAGCCTCTTCTCCACACTCAACTCATCCGGTTTTCAAGTCAGGCTCCTTT
ATCCGCTAGGCTGTCTTGTCAACCCTGAGTCAAACCTAAATGTAGTGAAGGAGGAAGCCAGAGTGGGCAT
CTCTGGGAAGAACAAGTCAGGCAAACAAAAACAACCAGCGTGTGTAAATGCCCTGAGGTAGGCTTGACTG
TGTCTGAGGACTAGAGAGGAAGCCTGTGTGGCTGATGTCAGGGGTGGGTGAGGGCAGAAGCTGAGGAAAG
CCATATTCGGTGTACCTGTGACTCATGGTGATAATTTAGGCATTTGTAGTAAAGGGGAAGGAAGAGTATC
ATAGCCGGCTATGGCCTCCAAAGGCTGCCCTCGGCCTTGTTTGTGCCACTCAGCTGCACAGGCCTTAGCC
CCACATGCAGTAGAGGTTCAGTAAACACTTGTCGGACAGATAGGATGTGTCGGCACACGTGGGGCCGTGG
ACATGGCATGATTTATTACGTCCCCCCCGTGAGTGCTCATGGGCCTTCTCAGTGCCTGTCGTGATGGATG
GTTGTAGTGTTTCTGTTATCGCAGGGGTCCTGGGGAGCCTGGGCTGGAGGCTGAGGGCAGCCTAGGGTTG
GAGGGGGCAGCAGGGTTGTCCCTGCTCCTGACCTTTGTTCCTCCCGCCTGCAGCTCCACAGAGAAGAACT
GCTGTGTACGGCAGCTGTACATTGACTTTAGGAAGGACCTGGGTTGGAAGTGGATCCACGAGCCCAAGGG
CTACCATGCCAACTTCTGTCTGGGGCCCTGCCCCTACATTTGGAGCCTGGACACACAGTACAGCAAGGTG
GGTCTGGCCTGTGGGACAGGCTGGAGCAGAGGCTGGGTGGTTGGGAGAGAATGAGAGGCTAGAGCAGTGG
GGGAGTCAAGGGAATAGGGTGACAGGGAAACAGAGGAGAGACAGAAATACAGAGAGGCAGAGTTGGAGAG
ATGGGAAGAAACAGATTAGGTGAGAAAGACAGGAGAGCTAGTGATGTGGAGAGACACAGAAATGGAGGTG
GGGAGGATTGGAGATGGGAACTAGAGTAAGTGACCAAGACAGACAGGGAGGGGAGATAAGATGAAGAGAG
GGAGAGAGGAAGAGGCAGAGATGGGGGCAGATGAGAGAGAGGTGGAACATTAGGAAGAGACATAAATAGA
AATAGACTAGGATAGGTATCGAGGCCGATGGAAGCTGGGGAGACTCAGAGATAAGAGACAGAAATGTAGA
GATCCTGGTAGGGAGAGAGACCGGGGAGTCCCTGGACACTGGCAGTGACTCTGGCCCACCCGCAGGTCCT
TGCCCTCTACAACCAACACAACCCGGGTGCTTCCGCATCACCGTGCTGCGTGCCGCAGGCTTTGGAGCCA
CTGCCCATCGTCTACTACGTGGGTCGCAAGCCCAAGGTGGAGCAGTTGTCCAACATGATCGTGCGCTCCT
GCAAGTGCAGCTGAGGCCCCGCCCCCGCCCCGCCCCGCCCCTCCCGGCAGGCCCGGCCCCGCCCCCGCCC
CGCCCGCAGTGCCCGGGGCTGTATTTAAGGACACCTGCACCCCCCCCAAGCCCATCTGGGGGCCCATTAA
AGGTGACAGAGG

Protein Sequence : Show Sequence

>gi|11024652|ref|NP_067589.1| transforming growth factor beta-1 precursor [Rattus norvegicus]
MPPSGLRLLPLLLPLPWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPP
GPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYDKTKDITHSIYMFFNTSDI
REAVPEPPLLSRAELRLQRFKSTVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQ
GDGIQGFRFSAHCSCDSKDNVLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRAL
DTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGA
SASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Molecule Role : Other
Related Vaccine(s): Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen

5. TNF-alpha

Gene Name : TNF-alpha
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 21926
NCBI Protein GI : 7305585
Genbank Accession : AB039224
Protein Accession : NP_038721
Taxonomy ID : 10090
Chromosome No : 17
Gene Starting Position : 35199389
Gene Ending Position : 35201995
Gene Strand (Orientation) : -
Protein Name : tumor necrosis factor
Protein pI : 4.75
Protein Weight : 24010.47
Protein Length : 235
Protein Note : Also known as DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434

DNA Sequence : Show Sequence

>gi|372099093:35199389-35201995 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
CTTTATTTCTCTCAATGACCCGTAGGGCGATTACAGTCACGGCTCCCGTGGGGAGCAGAGGTTCAGTGAT
GTAGCGACAGCCTGGTCACCAAATCAGCGTTATTAAGACAATTGGGTTAGATAAATATTTTGTTTTAAAC
ATAAGCAAAAGAGGAGGCAACAAGGTAGAGAGGCCAGGTGGGGACAGCTCAGCTCCGTTTTCACAGAAAA
CATGTCTGTCTGAAGACAGCTTCCCACACTGGGTCCTCCAGGACACCCCGGCCTTCCAAATAAATACATT
CATAAGCAAATAAATAAATAATAAATAAATAATAAATAATAAGTGCAAATATAAATAGAGGGGGGCTGGC
TCTGTGAGGAAGGCTGTGCATTGCACCTCAGGGAAGAATCTGGAAAGGTCTGAAGGTAGGAAGGCCTGAG
ATCTTATCCAGCCTCATTCTGAGACAGAGGCAACCTGACCACTCTCCCTTTGCAGAACTCAGGAATGGAC
ATTCGAGGCTCCAGTGAATTCGGAAAGCCCATTTGAGTCCTTGATGGTGGTGCATGAGAGGCCCACAGTC
CAGGTCACTGTCCCAGCATCTTGTGTTTCTGAGTAGTTGTTGAAAGCTCTGAGCACAGAGTTGGACCCTG
AGCCATAATCCCCTTTCTAAGTTAGAAGGATACAGACTGGGGGCTCTGAGGAGTAGACAATAAAGGGGTC
AGAGTAAAGGGGTCAGAGTGGGGGCTGGGTAGAGAATGGATGAACACCCATTCCCTTCACAGAGCAATGA
CTCCAAAGTAGACCTGCCCGGACTCCGCAAAGTCTAAGTACTTGGGCAGATTGACCTCAGCGCTGAGTTG
GTCCCCCTTCTCCAGCTGGAAGACTCCTCCCAGGTATATGGGCTCATACCAGGGTTTGAGCTCAGCCCCC
TCAGGGGTGTCCTTGGGGCAGGGGCTCTTGACGGCAGAGAGGAGGTTGACTTTCTCCTGGTATGAGATAG
CAAATCGGCTGACGGTGTGGGTGAGGAGCACGTAGTCGGGGCAGCCTTGTCCCTTGAAGAGAACCTGGGA
GTAGACAAGGTACAACCCATCGGCTGGCACCACTAGTTGGTTGTCTTTGAGATCCATGCCGTTGGCCAGG
AGGGCGTTGGCGCGCTGGCTCAGCCACTCCAGCTGCTCCTCCACTTGGTGGTTTGCTGAGGGGGGGGGGG
AGGATTGAGTCAGTGTCACCCTCTTAGTTCACACTCCACATCCTGAGCCTCAGCAGCTACCCACACTTCA
CTTCCGGTTCCTGCACCCTCTGTCTTTCCACATCCCATTGGCTATGAGGTCCCGGGTGGCCCCCTGATGC
CTTGCTTTTGAGTCACTGCTCTGACTCTCACGTGCTGTCTCTAAGAGCTCTGTCTTTTCTCAGCCTGGCT
CGACACCCCTCAACCCGCCCCCCAAAATCATGCCCCTTCATTCTCAAGGCACATGTAAAGAAATCTTACC
TACGACGTGGGCTACAGGCTTGTCACTCGAATTTTGAGAAGATGATCCTGGAGGGGAAGAGACAAAGGCA
AGGATGAGCCTTTTAGGCTTCCCAGCAAGCATCTATGCACTTAGACCCCTTTCCTCCCAAACCAAAGCTT
TAAGTTCTCCCCCCACCCCATCTCATCCCATGCCTAACTGCCCTTCCTCCATCTTAAATTAAGAGAGAGG
TGTGGGAACACTTACTGAGTGTGAGGGTCTGGGCCATAGAACTGATGAGAGGGAGGCCATTTGGGAACTT
CTGTGTAGGAAAAGGAGGTTAGTTAAGACAGACTCACCCCAAAGGAGAAGCCTCCCGGCTGATTGCCCCG
CTTACAGTTCCTCTTTGCCCCACCCCACCCCCCAGCTTTGTGTTTTTCTTCTTCATTCATTCATCTGTCC
AACCCACGGCTTCTTTCTGCGGTGCCCTCTGTGCTTGATCTCCCGTTATCTCCCCTTCATCTTCCTCCTT
ATCTCTCATGCCTCTCTCATTTCTGTCTCTGAGTTTTATCTCTTGCTTATCCCCTCTTCCCCTGGCCACA
TCTTTCCAGATCTCTCCACGTGTGAACACACTTGTTCGTTCATTCATCTCTCTGTGCATCCGACGAAGGA
TGTTTAGTCAGCTGGACGCATGGGTCCGAGGTCCTGACTCTGTCCCCTCCACACTCTCCTCCACCTTGCC
CTGCCCATTAGCCCACTTCTTTCCCTCACACTGTCCTTCTTGCCCTCCTAACCCGTTTTGCTTGTGAGCG
AGAATAAGGGTTGCCCAGACACTCACCTCATCCCTTTGGGGACCGATCACCCCGAAGTTCAGTAGACAGA
AGAGCGTGGTGGCCCCTGCCACAAGCAGGAATGAGAAGAGGCTGAGACATAGGCACCGCCTGGAGTTCTG
GAAGCCCCCCATCTTTTGGGGGAGTGCCTCTTCTGCCAGTTCCACGTCGCGGATCATGCTTTCTGTGCTC
ATGGTGTCTTTTCTGGAGGGAGATGTGGCGCCTTGGGCCAGTGAGTGAAAGGGACAGAACCTGCCTGGTT
GGCTGCTTGCTTTTCTGGGAGCTATTTCCAAGATGTTCTGGAGTTTCTGTTCTCCCTCCTGGCTAGTCCC
TTGCTGTCCTCGCTGAG

Protein Sequence : Show Sequence

>gi|7305585|ref|NP_038721.1| tumor necrosis factor [Mus musculus]
MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPL
ISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQ
VLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQL
SAEVNLPKYLDFAESGQVYFGVIAL

Molecule Role : Vaximmutor
Related Vaccine(s): Adjuvant arthritis DNA vaccine CII encoding type II collagen

6. TNF-alpha from Rattus norvegicus

Gene Name : TNF-alpha from Rattus norvegicus
Sequence Strain (Species/Organism) : Rattus norvegicus
NCBI Gene ID : 24835
NCBI Protein GI : 6981662
Genbank Accession : AABR06099883
Protein Accession : NP_036807
Taxonomy ID : 10116
Chromosome No : 20
Gene Starting Position : 6936228
Gene Ending Position : 6938846
Gene Strand (Orientation) : +
Protein Name : tumor necrosis factor
Protein pI : 4.9
Protein Weight : 24217.89
Protein Length : 235
Protein Note : Also known as Tnfa; RATTNF; TNF-alpha

DNA Sequence : Show Sequence

>gi|389675109:6936228-6938846 Rattus norvegicus strain BN/SsNHsdMCW chromosome 20, Rnor_5.0
ACACCAAGGGACCAGCCAGGAGGGAGAACAGCAACTCCAGAACACCCTGGTACTAACTCCCAGAAAAGCA
AGCAACCAGCCAGGCAGGTTCCGTCCCTCTCATACACTGGCCCGAGGCAACACATCTCCCTCCGGAAAGG
ACACCATGAGCACGGAAAGCATGATCCGAGATGTGGAACTGGCAGAGGAGGCGCTCCCCAAAAAGATGGG
GGGCCTCCAGAACTCCAGGCGGTGTCTGTGCCTCAGCCTCTTCTCATTCCTGCTCGTGGCGGGGGCCACC
ACGCTCTTCTGTCTACTGAACTTCGGGGTGATCGGTCCCAACAAGGAGGAGGTGAGTGCCTGGGCAGCGT
TTATTCTCACTCACAAGCAAAGTAGGGTGGGAGGGCAAGAAAGGCAGGAGGGTAAGGGAAAGAGGTCGGC
TGGTGGGCACAGTAATGTGGAGGAGAGTTGGGGAGGGAGACCTCGGACCCGTGCAGCCAGTTGTCTAAAC
ATCCTTGGGTGGATGCACGGAGGGGCGAATGAACGAACAAGCGTGCGTACACGTGCAGAGATGTGCAGAG
ACGTGGCCAGGGGAACAGGGAAGAAGCAAGAGATAAAAATTCGAGACGGAGATGGGAGACGAGGGAGATA
AGGAGATATGAGAGATAAGGAGGGAGATGGAGGGGAAACAACAGATCAAGCACAGAGGGTACCACAGAAA
GATGCTGAGGTTGGACGGATAAATGAATGAGGAGGAAAAGCACTGACTGGGGGGGGGGGGCTGGCAAAGA
GCGGGGAGGCTTCTCCTTTGTGGTGAGTCTGTCTACTAACCTACTTCTTTCCCTACACAGAAGTTCCCAA
ATGGGCTCCCTCTCATCAGTTCCATGGCCCAGACCCTCACACTCAGTAAGTGTTCCCAAGCCTCTCTCTT
AATTTAGGATGGGGGGCGGGGGACTTAAAGCTTTGGTTTGGGAGGAAAGGGAATAGGGGTCTAACTACAC
AGATGCTTGCTGGGAAACCTAAGGGTCTCATCTCCGCCTTTGTCCCCTTCCAGGATCATCTTCTCAAAAC
TCGAGTGACAAGCCCGTAGCCCACGTCGTAGGTAAGTTTCTTCGCATGTGCCTTGGGGGGGGGGGTGTGA
CAGGGCAGGATTTTGGGGTCGTGCCAGGCTGAGAGAAGACAAGGGCTCTCAGAGACAGCAGGTGAGAGAG
TCAGAGCGGTGATTCAAAGGCAAGGCATTAGGAGGCCACCCCGGACCTCGTAGCCAATGGGACGTGGAAA
GACCAGAGGGCGCAGGAACCGGAAGTGAAGTGTGGGTAGAAGTTGAGGTTCAGGATGTGGAGTGTGAACT
AAGAGGGTGACACTGACTCAATTCTCCCTCCCTCAGCAAACCACCAAGCGGAGGAGCAGCTGGAGTGGCT
GAGCCAGCGTGCCAACGCCCTCCTGGCCAATGGCATGGATCTCAAAGACAACCAACTGGTGGTACCAGCA
GATGGGCTGTACCTTATCTACTCCCAGGTTCTCTTCAAGGGACAAGGCTGCCCCGACTACGTGCTCCTCA
CCCACACCGTCAGCCGATTTGCCATTTCATACCAGGAGAAAGTCAGCCTCCTCTCTGCCATCAAGAGCCC
TTGCCCTAAGGACACCCCTGAGGGAGCTGAGCTCAAGCCCTGGTATGAGCCCATGTACCTGGGAGGAGTC
TTCCAGCTGGAGAAGGGGGACCTGCTCAGCGCTGAGGTCAACCTGCCCAAGTACTTAGACATCACGGAGT
CCGGGCAGGTCTACTTTGGAGTCATTGCTCTGTGAGGCGACTGGCGTGTTCATCCGTTCTCTACCCAGCC
CCTGTCCCCGACTCTGACCCCCATTACTCTGACCCCTTTATCGTCTACTCCTCAGAGCCCCCAATCTGTG
TCCTTCTAACTTAGAAAGGGAATTGTGGCTCTGGGTCCAACTCCGGGCTCAGAATTTCCAACAACTACGA
TGCTCAGAAACACACGAGACGCTGAAGTAGTGGCCTGGATTGCGGGCTGCTCATGAGCCGCCACCGGCAA
GGATTCCAATGGGCTTTCGGAACTCACTGGATCCCGGAATGTCGATGCCTGAGTGGATCGGGGGAGTGGA
GGGGGGAGTGGATCAGGCTGCCTCTGGCTCACAAGGCTGCTGAAGATGTCTCAGGCCTCCCTTCCTTCCT
TCCTTCCCTCCCTCAGAGCTTCCCAGACTGTTCCCTGAGGCACAGCGCCCAGCCTTCCTTACGGAACCCC
CTCTATTTATAATTGCACCTGTGACTATTTATTTATTATTTATTATTTATTTATTTGCTTATGAATGTAT
TTATTTGGAAGGCCGGGGCGTCCTGGAGGACCCAGCGTTGGGAAGCTGTCTTCAGGCCAACATGTTTTCT
GTGAAAACGGAGCTAAACTACCAGCTATCCCCATCTGGTCCCCCACCCCTGCCTCGTTGCCTCCCCCCTT
TTCTTTTGCTTATGTTTAAAACAAGATATTTATCTAACCCAGTTGTCTTAATAATGCTGATTTGGTGACC
AGGCTGTCGCTACATCACTGAACCTCTGCTCCCCACGGGAGCCGTGACTGTAATCGCCCTACGGGTCATT
GAGAGAAATAAAGTTCGCTTGGAAAAGAA

Protein Sequence : Show Sequence

>gi|6981662|ref|NP_036807.1| tumor necrosis factor [Rattus norvegicus]
MSTESMIRDVELAEEALPKKMGGLQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPNKEEKFPNGLPL
ISSMAQTLTLRSSSQNSSDKPVAHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQ
VLFKGQGCPDYVLLTHTVSRFAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLL
SAEVNLPKYLDITESGQVYFGVIAL

Molecule Role : Other
Related Vaccine(s): Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen

IV. Vaccine Information

1. Adjuvant arthritis DNA vaccine CII encoding type II collagen

a. Vaccine Ontology ID:

VO_0004409

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of Collagen type II

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Vaccine Immune Response Type: VO_0000286
Immune Response: In vitro analysis of anti-CII T cells demonstrated reduced production of the pro-inflammatory cytokines IFN-γ and TNF-α in CII vaccinated mice. Further, CII DNA vaccines reduced epitope spreading of auto-reactive B cell responses in CIA (Ho et al., 2006).
Efficacy: DNA encoding CII reduced the disease severity and disease incidence of collagen-induced arthritis to 50% as compared to the PBS-treated control group in which the disease was more severe and the disease incidence was 89% (Ho et al., 2006).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: CII-stimulated lymphocytes isolated from CIA mice treated with a DNA vaccine encoding CII produced significantly less IFN-g and TNF-a compared to PBS and DNA vector-IL-4 treated mice (Ho et al., 2006).
- Detailed Gene Information: Click here.
Host Gene Response of TNF-alpha
- Gene Response: CII-stimulated lymphocytes isolated from CIA mice treated with a DNA vaccine encoding CII produced significantly less IFN-g and TNF-a compared to PBS and DNA vector-IL-4 treated mice (Ho et al., 2006).
- Detailed Gene Information: Click here.

2. Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen

a. Vaccine Ontology ID:

VO_0004410

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Rat

e. Gene Engineering of Collagen type II

Type: DNA vaccine construction
Description: Vector pcDNA3 expressed chicken type II collagen (CCOL2A1) (Song et al., 2009).
Detailed Gene Information: Click here.

f. Vector:

pcDNA3 (Song et al., 2009)

g. Immunization Route

Intramuscular injection (i.m.)

h. Rat Response

Vaccine Immune Response Type: VO_0000286
Immune Response: CD4+CD25+Treg cells play a role in restraining auto-aggressive T cells in experimental settings, and 200 μg/kg of pcDNA-CCOL2A1vaccine increased the proportion of CD4+CD25+Treg cells in the blood of arthritic rats compared with those in all other groups (Song et al., 2009).
Efficacy: Comparison of all treated and control groups demonstrated clearly that 200 μg/kg of pcDNA-CCOL2A1 vaccine alone in a single intravenous treatment significantly reduced the severity of disease, footpad swelling, arthritic incidence, clinical scores, and deferred the onset of disease (Song et al., 2009).
Host Gene Response of IgG (partial)
- Gene Response: pcDNA-CCOL2A1 vaccine significantly reduced the levels of serum anti-CII IgG antibodies compared to the negative control group (P < 0.05) (Song et al., 2009).
- Detailed Gene Information: Click here.
Host Gene Response of Il10 from Rattus norvegicus
- Gene Response: Concentrations of the Th2-cytokine IL-10 in 200 μg/kg of pcDNA-CCOL2A1 vaccine group were significantly higher than those in all other groups, including the MTX positive control group (P < 0.05) (Song et al., 2009).
- Detailed Gene Information: Click here.
Host Gene Response of TGF-B from Rattus norvegicus
- Gene Response: Concentrations of the Th3-cytokine TGF-β in 200 μg/kg of pcDNA-CCOL2A1 vaccine group were significantly higher than those in all other groups, including the MTX positive control group (P < 0.05) (Song et al., 2009).
- Detailed Gene Information: Click here.
Host Gene Response of TNF-alpha from Rattus norvegicus
- Gene Response: Concentrations of the Th1-cytokine TNF-α in both 200 μg/kg of pcDNA-CCOL2A1 vaccine and MTX groups were significantly lower than those in the negative control and other treatment groups (P < 0.05). Of note, the level of TNF-α in 200 μg/kg of pcDNA-CCOL2A1 vaccine group recovered to that of the normal group (Song et al., 2009).
- Detailed Gene Information: Click here.

3. Adjuvant arthritis DNA vaccine pCMV3.65 encoding HSP65

a. Vaccine Ontology ID:

VO_0004407

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Rat

e. Gene Engineering of hsp65

Type: DNA vaccine construction
Description: Vector pcDNA3 expressed mycobacterial heat shock protein 65 (hsp65) (Ragno et al., 1997).
Detailed Gene Information: Click here.

f. Vector:

pcDNA3 (Ragno et al., 1997)

g. Immunization Route

Intramuscular injection (i.m.)

h. Rat Response

Vaccine Immune Response Type: VO_0000286
Immune Response: Rats treated with pCMV3.65 showed a significantly elevated antibody levels (median 0.707) when compared with vector-treated rats (median 0.351; P < 0.01) and with naive animals (median 0.0715; P < 0.01). Similarly, spleen cells from pCMV3.65-treated rats exhibited significantly higher proliferative responses against the mycobacterial recombinant antigen when compared with vector treated animals (P < 0.05) or with naive rats (P < 0.01) (Ragno et al., 1997).
Efficacy: The pCMV3.65-treated rats were significantly protected from disease development in comparison with the control groups injected with the empty plasmid (Ragno et al., 1997).

4. Pristane-induced arthritis DNA vaccine DNA-hsp65 encoding Mycobacterium leprae 65-kDa heat shock protein

a. Vaccine Ontology ID:

VO_0004408

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of hsp65

Type: DNA vaccine construction
Description: Vector pcDNA3 expressed hsp65 from M. leprae (Santos-Junior et al., 2005).
Detailed Gene Information: Click here.

f. Vector:

pcDNA3 (Santos-Junior et al., 2005)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Immune Response: Cellular and humoral immune responses to the protein are elicited, and long-lived memory lymphocytes are induced (Santos-Junior et al., 2005).
Efficacy: DNA-hsp65 vaccination was protective against arthritis in AIRmax mice (maximal acute inflammatory reaction), as evidenced by the absence of arthritis incidence during the 150-day follow-up period. This protective effect was more pronounced after the onset of pristane-induced arthritis (Santos-Junior et al., 2005).

V. References

1. Ho et al., 2006: Ho PP, Higgins JP, Kidd BA, Tomooka B, Digennaro C, Lee LY, de Vegvar HE, Steinman L, Robinson WH. Tolerizing DNA vaccines for autoimmune arthritis. Autoimmunity. 2006; 39(8); 675-682. [PubMed: 17178564].

2. Mayo Clinic - Arthritis: Arthritis [http://www.mayoclinic.com/health/arthritis/DS01122]

3. Quintana et al., 2002: Quintana FJ, Carmi P, Mor F, Cohen IR. Inhibition of adjuvant arthritis by a DNA vaccine encoding human heat shock protein 60. Journal of immunology (Baltimore, Md. : 1950). 2002; 169(6); 3422-3428. [PubMed: 12218165].

4. Ragno et al., 1997: Ragno S, Colston MJ, Lowrie DB, Winrow VR, Blake DR, Tascon R. Protection of rats from adjuvant arthritis by immunization with naked DNA encoding for mycobacterial heat shock protein 65. Arthritis and rheumatism. 1997; 40(2); 277-283. [PubMed: 9041939].

5. Santos-Junior et al., 2005: Santos-Junior RR, Sartori A, De Franco M, Filho OG, Coelho-Castelo AA, Bonato VL, Cabrera WH, IbaÃ±ez OM, Silva CL. Immunomodulation and protection induced by DNA-hsp65 vaccination in an animal model of arthritis. Human gene therapy. 2005; 16(11); 1338-1345. [PubMed: 16259568].

6. Song et al., 2009: Song X, Liang F, Liu N, Luo Y, Xue H, Yuan F, Tan L, Sun Y, Xi C, Xi Y. Construction and characterization of a novel DNA vaccine that is potent antigen-specific tolerizing therapy for experimental arthritis by increasing CD4+CD25+Treg cells and inducing Th1 to Th2 shift in both cells and cytokines. Vaccine. 2009; 27(5); 690-700. [PubMed: 19095031].