VIOLIN: Vaccine Investigation and Online Information Network

Hepatitis E virus

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. ORF-2 (Other)
2. Hepatitis E Virus E2s Domain (Protective antigen)
3. ORF1 (Protective antigen)
4. ORF2 (Protective antigen)
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

12461

2. Disease:

Hepatitis E

3. Introduction

Hepatitis E is a serious liver disease caused by the Hepatitis E virus (HEV) that usually results in an acute infection. It does not lead to a chronic infection. While rare in the United States, Hepatitis E is common in many parts of the world. Transmission: Ingestion of fecal matter, even in microscopic amounts; outbreaks are usually associated with contaminated water supply in countries with poor sanitation (CDC - Hepatitis E).

II. Vaccine Related Pathogen Genes

1. Hepatitis E Virus E2s Domain

Gene Name : Hepatitis E Virus E2s Domain
Sequence Strain (Species/Organism) : Hepatitis E virus
NCBI Protein GI : 3RKD_B
Other Database IDs : CDD:281067
Taxonomy ID : 12461
Protein Name : Hepatitis E Virus E2s Domain
Protein pI : 8.67
Protein Weight : 14713.19
Protein Length : 186
Protein Note : Structural protein 2; pfam03014

Protein Sequence : Show Sequence

>pdb|3RKD|B Chain B, Capsid Protein
SRPFSVLRANDVLWLSLTAAEYDQSTYGSSTGPVYVSDSVTLVNVATGAQAVARSLDWTKVTLDGRPLST
IQQHSKTFFVLPLRGKLSFWEAGTTKAGYPYNYNTTASDQLLVENAAGHRVAISTYTTSLGAGPVSISAV
AVLAPP

Molecule Role : Protective antigen
Molecule Role Annotation : (Li et al., 2009)

2. ORF-2

Gene Name : ORF-2
Sequence Strain (Species/Organism) : Hepatitis E virus
NCBI Gene ID : 1494410
NCBI Protein GI : 9626450
Locus Tag : HEVgp10
Genbank Accession : L08816
Protein Accession : NP_056788
Taxonomy ID : 12461
Gene Starting Position : 5122
Gene Ending Position : 7104
Gene Strand (Orientation) : +
Protein Name : capsid protein
Protein pI : 9.05
Protein Weight : 66613.67
Protein Length : 660

DNA Sequence : Show Sequence

>gi|9626440:5122-7104 Hepatitis E virus, complete genome
CATGCGCCCTCGGCCTATTTTGCTGTTGCTCCTCATGTTTCTGCCTATGCTGCCCGCGCCACCGCCCGGT
CAGCCGTCTGGCCGCCGCCGTGGGCGGCGCAGCGGCGGTTCCGGCGGTGGTTTCTGGGGTGACCGGGCTG
ATTCTCAGCCCTTCGCAATCCCCTATATTCATCCAACCAACCCCTTCGCCCCCGATGTCACCGCTGCGGC
CGGGGCTGGACCTCGTGTTCGCCAACCCGCCCGACCACTCGGCTCCGCTTGGCGTGACCAGGCCCAGCGC
CCCGCCGCTGCCTCACGTCGTAGACCTACCACAGCTGGGGCCGCGCCGCTAACCGCGGTCGCTCCGGCCC
ATGACACCCCGCCAGTGCCTGATGTTGACTCCCGCGGCGCCATCCTGCGCCGGCAGTATAACCTATCAAC
ATCTCCCCTTACCTCTTCCGTGGCCACCGGTACAAACTTGGTTCTTTACGCCGCTCCTCTTAGCCCGCTT
CTACCCCTCCAGGACGGCACCAATACTCATATAATGGCTACAGAAGCTTCTAATTATGCCCAGTACCGGG
TTGTTCGTGCTACAATTCGCTACCGCCCGCTGGTCCCCAACGCTGTTGGTGGCTACGCCATCTCCATCTC
GTTCTGGCCACAGACCACCACCACCCCGACGTCCGTTGACATGAATTCAATAACCTCGACTGATGTTCGT
ATTTTAGTCCAGCCCGGCATAGCCTCCGAGCATGTTATCCCAAGTGAGCGCCTACACTATCGTAACCAAG
GTTGGCGCTCTGTTGAGACCTCCGGGGTGGCGGAGGAGGAGGCCACCTCTGGTCTTGTTATGCTTTGCAT
ACATGGCTCACTCGTAAACTCTTATACTAATACACCTTATACCGGTGCCCTCGGGCTGTTGGACTTTGCC
CTCGAACTTGAGTTCCGCAACCTCACCCCCGGTAATACCAACACGCGGGTCTCCCGTTACTCCAGCACTG
CCCGTCACCGCCTTCGTCGCGGTGCAGATGGGACTGCCGAGCTCACCACCACGGCTGCTACCCGCTTCAT
GAAGGACCTCTATTTTACTAGTACTAATGGTGTCGGTGAGATCGGCCGCGGGATAGCGCTTACCCTGTTT
AACCTTGCTGACACCCTGCTTGGCGGTCTACCGACAGAATTGATTTCGTCGGCTGGTGGCCAGCTGTTCT
ACTCTCGCCCCGTCGTCTCAGCCAATGGCGAGCCGACTGTTAAGCTGTATACATCTGTAGAGAATGCTCA
GCAGGATAAGGGTATTGCAATCCCGCATGACATCGACCTCGGGGAATCTCGTGTAGTTATTCAGGATTAT
GATAACCAACATGAGCAGGACCGACCGACACCTTCCCCAGCCCCATCGCGCCCCTTTTCTGTCCTCCGAG
CTAATGATGTGCTTTGGCTTTCTCTCACCGCTGCCGAGTATGACCAGTCCACTTACGGCTCTTCGACCGG
CCCAGTCTATGTCTCTGACTCTGTGACCTTGGTTAATGTAGCGACCGGCGCGCAGGCCGTTGCCCGGTCG
CTCGACTGGACCAAGGTCACACTTGATGGTCGCCCCCTTTCCACCACCCAGCAGTATTCAAAGACCTTCT
TTGTCCTGCCGCTCCGCGGTAAGCTCTCCTTTTGGGAGGCAGGTACTACTAAAGCCGGGTACCCTTATAA
TTATAACACCACTGCTAGTGACCAACTGCTCGTTGAGAATGCCGCTGGGCATCGGGTTGCTATTTCCACT
TACACCACTAGCCTGGGTGCTGGCCCCGTCTCTATTTCCGCGGTTGCTGTTTTAGCCCCCCACTCTGCGC
TAGCATTGCTTGAGGATACCATGGACTACCCTGCCCGCGCCCATACTTTCGATGACTTCTGCCCGGAGTG
CCGCCCCCTTGGCCTCCAGGGCTGTGCTTTTCAGTCTACTGTCGCTGAGCTTCAGCGCCTTAAGATGAAG
GTGGGTAAAACTCGGGAGTTATA

Protein Sequence : Show Sequence

>gi|9626450|ref|NP_056788.1| capsid protein [Hepatitis E virus]
MRPRPILLLLLMFLPMLPAPPPGQPSGRRRGRRSGGSGGGFWGDRADSQPFAIPYIHPTNPFAPDVTAAA
GAGPRVRQPARPLGSAWRDQAQRPAAASRRRPTTAGAAPLTAVAPAHDTPPVPDVDSRGAILRRQYNLST
SPLTSSVATGTNLVLYAAPLSPLLPLQDGTNTHIMATEASNYAQYRVVRATIRYRPLVPNAVGGYAISIS
FWPQTTTTPTSVDMNSITSTDVRILVQPGIASEHVIPSERLHYRNQGWRSVETSGVAEEEATSGLVMLCI
HGSLVNSYTNTPYTGALGLLDFALELEFRNLTPGNTNTRVSRYSSTARHRLRRGADGTAELTTTAATRFM
KDLYFTSTNGVGEIGRGIALTLFNLADTLLGGLPTELISSAGGQLFYSRPVVSANGEPTVKLYTSVENAQ
QDKGIAIPHDIDLGESRVVIQDYDNQHEQDRPTPSPAPSRPFSVLRANDVLWLSLTAAEYDQSTYGSSTG
PVYVSDSVTLVNVATGAQAVARSLDWTKVTLDGRPLSTTQQYSKTFFVLPLRGKLSFWEAGTTKAGYPYN
YNTTASDQLLVENAAGHRVAISTYTTSLGAGPVSISAVAVLAPHSALALLEDTMDYPARAHTFDDFCPEC
RPLGLQGCAFQSTVAELQRLKMKVGKTREL

Molecule Role : Other

3. ORF1

Gene Name : ORF1
Sequence Strain (Species/Organism) : Hepatitis E virus
NCBI Protein GI : BAD35096
Other Database IDs : CDD:110646
Taxonomy ID : 12461
Protein Name : ORF1
Protein pI : 11.17
Protein Weight : 14981.95
Protein Length : 186
Protein Note : genotype 4

Protein Sequence : Show Sequence

>BAD35096.1 ORF1, partial [Hepatitis E virus]
QFIKAPGVTTAIEQAALAAANSALANAVVVRPFLSRLQTEILINLMQPRQLVFRPEVLWNHPIQRVIHNE
LEQYCRARAGRCLEVGAHPRSINDNPNVLHRCFLKPVGRDVQRWYTAPTRGPAANCRRSALRGLPPA

Molecule Role : Protective antigen
Molecule Role Annotation : (Al-Ayoubi et al., 2018)

4. ORF2

Gene Name : ORF2
Sequence Strain (Species/Organism) : Hepatitis E virus
NCBI Protein GI : AAA03191
Other Database IDs : CDD:281067
Taxonomy ID : 12461
Protein Name : ORF2
Protein pI : 9.05
Protein Weight : 67396.48
Protein Length : 709
Protein Note : Structural protein 2; pfam03014

Protein Sequence : Show Sequence

>AAA03191.1 ORF 2 [Hepatitis E virus]
MRPRPILLLLLMFLPMLPAPPPGQPSGRRRGRRSGGSGGGFWGDRADSQPFAIPYIHPTNPFAPDVTAAA
GAGPRVRQPARPLGSAWRDQAQRPAAASRRRPTTAGAAPLTAVAPAHDTPPVPDVDSRGAILRRQYNLST
SPLTSSVATGTNLVLYAAPLSPLLPLQDGTNTHIMATEASNYAQYRVVRATIRYRPLVPNAVGGYAISIS
FWPQTTTTPTSVDMNSITSTDVRILVQPGIASEHVIPSERLHYRNQGWRSVETSGVAEEEATSGLVMLCI
HGSLVNSYTNTPYTGALGLLDFALELEFRNLTPGNTNTRVSRYSSTARHRLRRGADGTAELTTTAATRFM
KDLYFTSTNGVGEIGRGIALTLFNLADTLLGGLPTELISSAGGQLFYSRPVVSANGEPTVKLYTSVENAQ
QDKGIAIPHDIDLGESRVVIQDYDNQHEQDRPTPSPAPSRPFSVLRANDVLWLSLTAAEYDQSTYGSSTG
PVYVSDSVTLVNVATGAQAVARSLDWTKVTLDGRPLSTTQQYSKTFFVLPLRGKLSFWEAGTTKAGYPYN
YNTTASDQLLVENAAGHRVAISTYTTSLGAGPVSISAVAVLAPHSALALLEDTMDYPARAHTFDDFCPEC
RPLGLQGCAFQSTVAELQRLKMKVGKTREL

Molecule Role : Protective antigen
Molecule Role Annotation : (Joshi and Arankalle, 2016)

III. Vaccine Information

IV. References

1. Al-Ayoubi et al., 2018: Al-Ayoubi J, Behrendt P, Bremer B, Suneetha PV, Gisa A, Rinker F, Manns MP, Cornberg M, Wedemeyer H, Kraft ARM. Hepatitis E virus ORF 1 induces proliferative and functional T-cell responses in patients with ongoing and resolved hepatitis E. Liver international : official journal of the International Association for the Study of the Liver. 2018; 38(2); 266-277. [PubMed: 28718943].

2. CDC - Hepatitis E: Hepatitis E Information for Health Professionals [http://www.cdc.gov/hepatitis/HEV/]

3. He et al., 1997: He J, Hoffman SL, Hayes CG. DNA inoculation with a plasmid vector carrying the hepatitis E virus structural protein gene induces immune response in mice. Vaccine. 1997; 15(4); 357-362. [PubMed: 9141205].

4. Joshi and Arankalle, 2016: Joshi SS, Arankalle VA. Differential Immune Responses in Mice Immunized with Recombinant Neutralizing Epitope Protein of Hepatitis E Virus Formulated with Liposome and Alum Adjuvants. Viral immunology. 2016; 29(6); 350-360. [PubMed: 27285290].

5. Li et al., 2009: Li S, Tang X, Seetharaman J, Yang C, Gu Y, Zhang J, Du H, Shih JW, Hew CL, Sivaraman J, Xia N. Dimerization of hepatitis E virus capsid protein E2s domain is essential for virus-host interaction. PLoS pathogens. 2009; 5(8); e1000537. [PubMed: 19662165].