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Viral haemorrhagic septicaemia virus |
Table of Contents |
- General Information
- NCBI Taxonomy ID
- Introduction
- Vaccine Related Pathogen Genes
- Glycoprotein
(Protective antigen)
- glycoprotein
(Protective antigen)
- nucleocapsid
(Protective antigen)
- Vaccine Information
- VHSV DNA vaccine pCMV-vhsG encoding glycoprotein G
- VHSV DNA vaccine pCMV-vhsN encoding nucleocapsid protein N and glycoprotein G
- VHSV DNA vaccine pVHS-G encoding glycoproteins
- References
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I. General Information |
1. NCBI Taxonomy ID: |
11287 |
2. Introduction |
Viral Hemorrhagic Septicemia (VHS) is a deadly fish virus and aquatic invasive species that attacks and weakens the blood vessels of fish. Broken blood vessels and severe blood loss ultimately causes death. It afflicts more than 50 species of freshwater and marine fish in several parts of the northern hemisphere. Scientists are not sure how the virus arrived. It may have come in with migrating fish, or may have hitchhiked in ballast water from ships. VHS was first found in European freshwater trout in the late 1930s and continues to cause epidemics in European trout farms. It appeared on the U.S. West Coast in 1988 in marine trout and salmon (WISC - VHSV). |
II. Vaccine Related Pathogen Genes |
1. Glycoprotein |
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Gene Name :
Glycoprotein
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Sequence Strain (Species/Organism) :
Viral hemorrhagic septicemia virus Danish isolate DK-3592B
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NCBI Protein GI :
299444
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Genbank Accession :
Y18263
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Protein Accession :
NP_049548
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Other Database IDs :
CDD:279337
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Taxonomy ID :
11287
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Gene Starting Position :
2958
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Gene Ending Position :
4532
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Gene Strand (Orientation) :
?
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Protein Name :
envelope glycoprotein
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Protein pI :
7.14
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Protein Weight :
54010.72
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Protein Length :
647
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Protein Note :
G protein
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DNA Sequence : Show Sequence
>gi|9632550:2958-4532 Viral hemorrhagic septicemia virus Fil3, complete genome
CATGGAATGGAACACTTTTTTCTTGGTCATCTTGATCATCATCATAAAGAGCACCACACCACAGATCACT
CAACGACCTCCGGTCGAAAACATCTCGACGTACCATGCAGATTGGGACACTCCGCTATACACTCATCCCT
CCAACTGCAGGGACGATTCCTTTGTCCCGATTCGACCAGCTCAACTCAGGTGTCCTCATGAATTTGAAGA
CATAAACAAGGGACTGGTCTCCGTCCCAACCAAGATCATCCATCTCCCGCTATCAGTCACCAGCGTCTCC
GCAGTCGCGAGTGGCCACTACCTGCACAGAGTGACTTATCGAGTCACCTGTTCGACCAGCTTCTTTGGAG
GGCAAACCATTGAAAAGACCATCTTAGAGGCGAAACTGTCTCGTCAGGAGGCCACAGACGAGGCAAGCAA
GGATCACGAGTACCCGTTCTTCCCTGAACCCTCCTGCATCTGGATGAAAAACAATGTCCATAAGGACATA
ACTCACTATTACAAGACCCCAAAAACAGTATCGGTGGATCTCTACAGCAGGAAATTTCTCAACCCTGATT
TCATAGAGGGGGTTTGCACAACCTCGCCCTGTCAAACTCATTGGCAGGGAGTCTATTGGGTCGGTGCCAC
ACCCAAAGCCCATTGCCCCACGTCGGAAACACTAGAAGGACACCTGTTCACCAGGACCCATGATCACAGG
GTGGTCAAGGCAATTGTGGCAGGCCATCATCCCTGGGGACTCACAATGGCATGCACAGTGACATTCTGCG
GGGCAGAATGGATCAAGACTGACCTGGGAGACCTGATCCAGGTAACAGGACCGGGGGGCACGGGGAAACT
GACTCCAAAGAAGTGTGTCAATGCTGATGTCCAGATGAGGGGGGCAACAGATGACTTTTCTTATCTCAAC
CATCTCATCACCAACATGGCTCAAAGAACCGAGTGCCTAGATGCCCATAGTGATATCACCGCTTCTGGGA
AAATATCCTCATTTCTCCTCTCAAAGTTTCGTCCCAGCCACCCTGGACCCGGCAAGGCACACTATCTTCT
CAACGGTCAAATCATGCGAGGTGACTGTGACTATGAGGCAGTAGTCAGCATCAACTACAACAGCGCTCAA
TACAAGACAGTGAACAACACATGGAAATCATGGAAACGGGTAGACAACAACACAGACGGGTACGATGGGA
TGATATTTGGGGACAAATTGATCATCCCGGACATCGAGAAGTATCAGAGTGTCTATGACAGTGGAATGCT
CGTTCAAAGAAACCTTGTGGAAGTCCCTCATCTGAGCATTGTGTTTGTCTCCAACACATCTGATCTTTCC
ACTAATCACATCCACACCAACCTAATCCCTTCGGATTGGTCATTCCACTGGAGTATTTGGCCCTCATTAT
CTGGGATGGGGGTTGTGGGAGGGGCCTTCCTTCTACTGGTACTCTGCTGTTGCTGCAAGGCGTCCCCTCC
CATTCCAAATTACGGGATTCCGATGCAGCAGTTCTCCAGAAGTCAGACGGTCTGAGCACACCTGTCCGAA
TGACCACAATTCCTCTATTAGGTAGATAGAAAAAA
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Protein Sequence : Show Sequence
>AAB26115.1 envelope glycoprotein, G protein [viral haemorrhagic septicaemia virus VHSV, Danish isolate DK-3592B, Peptide, 507 aa]
MEWNTFFLVILIIIIKSTTPQITQRPPVENISTYHADWDTPLYTHPSNCRDDSFVPIRPAQLRCPHEFED
INKGLVSVPTKIIHLPLSVTSVSAVASGHYLHRVTYRVTCSTSFFGGQTIEKTILEAKLSRQEATDEASK
DHEYPFFPEPSCIWMKNNVHKDITHYYKTPKTVSVDLYSRKFLNPDFIEGVCTTSPCQTHWQGVYWVGAT
PKAHCPTSETLEGHLFTRTHDHRVVKAIVAGHHPWGLTMACTVTFCGAEWIKTDLGDLIQVTGPGGTGKL
TPKKCVNADVQMRGATDDFSYLNHLITNMAQRTECLDAHSDITASGKISSFLLSKFRPSHPGPGKAHYLL
NGQIMRGDCDYEAVVSINYNSAQYKTVNNTWKSWKRVDNNTDGYDGMIFGDKLIIPDIEKYQSVYDSGML
VQRNLVEVPHLSTVFVSNTSDLSTNHIHTNLIPSDWSFHWSIWPSLSGMGVVGGAFLLLVLCCCCKASPP
IPNYGIPMQQFSRSQTV
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Molecule Role :
Protective antigen
- Related Vaccine(s):
VHSV DNA vaccine pCMV-vhsG encoding glycoprotein G
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VHSV DNA vaccine pCMV-vhsN encoding nucleocapsid protein N and glycoprotein G
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VHSV DNA vaccine pVHS-G encoding glycoproteins
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2. glycoprotein |
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Gene Name :
glycoprotein
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Sequence Strain (Species/Organism) :
Viral haemorrhagic septicaemia virus
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NCBI Protein GI :
CAB07737
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Other Database IDs :
CDD:279337
GOA:O42008 InterPro: IPR001903 InterPro: IPR002417 UniProtKB/TrEMBL: O42008
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Taxonomy ID :
11287
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Protein Name :
glycoprotein
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Protein pI :
7.1
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Protein Weight :
53554.36
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Protein Length :
578
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Protein Note :
Rhabdovirus spike glycoprotein; pfam00974
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Protein Sequence : Show Sequence
>CAB07737.2 glycoprotein [Viral hemorrhagic septicemia virus]
MEWNTFFLVILIIIIKSTTPQITQRPPVENISTYHADWDTPLYTHPSNCREDSFVPIRPAQLRCPHEFED
INKGLVSVPTQIIHLPLSVTSVSAVASGHYLHRVTYRVTCSTSFFGGQTIEKTILEAKLSRQEATNEASK
DHEYPFFPEPSCIWMKNNVHKDITHYYKTPKTVSVDLYSRKFLNPDFIEGVCTTSPCQTHWQGVYWVGAT
PTAHCPTSETLEGHLFTRTHDHRVVKAIVAGHHPWGLTMACTVTFCGTEWIKTDLGDLIQVTGPGGARKL
TPKKCVNTDIQMRGATDDFSYLNHLITNMAQRTECLDAHSDITASGKISSFLLSKFRPSHPGPGKAHYLL
DGQIMRGDCDYEAVVSINYNSAQYKTVNNTWKSWKRVDNNTDGYDGMIFGDKLIIPDIEKYQSVYDSGML
VQRNLVEVPHLSIVFVSNTSDLSTNHIHTNLIPSDWSFNWSLWPSLSGMGVVGGAFLLLVLCCCCKASPP
IPNYGIPMQQFSRSQMV
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Molecule Role :
Protective antigen
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Molecule Role Annotation :
(Standish et al., 2016)
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3. nucleocapsid |
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Gene Name :
nucleocapsid
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Sequence Strain (Species/Organism) :
Viral hemorrhagic septicemia virus DK-3592B
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NCBI Protein GI :
3282333
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Other Database IDs :
CDD:281242
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Taxonomy ID :
11287
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Gene Strand (Orientation) :
?
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Protein Name :
nucleocapsid protein
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Protein pI :
4.89
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Protein Weight :
41709.47
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Protein Length :
490
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Protein Note :
N protein
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Protein Sequence : Show Sequence
>AAC24962.1 nucleocapsid protein, partial [Viral hemorrhagic septicemia virus]
MEGGIRAAFSGLNDVRIDPTGGEGRVLVPGDVELIVYVGGFGEEDRKVIVDALSALGGPQTVQALSVLLS
YVLQGNTQEDLETKCKVLTDMGFKVTQAVRATSIEAGIMMPMRELALTVNDDNLMEIVKGTLMTCSLLTK
YSVDKMIKYITKKLGELADTQGVGELQHFTADKAAIRKLAGCVRPGQKITKALYAFILTEIADPTTQSRA
RAMGALRLNGTGMTMIGLFTQAANNLGIAPAKLLEDLCMESLVESARRIIQLMRQVSEAKSIQERYAIMM
SRMLGESYYKSYGLNDNSKISYILSQISGKYAVDSLEGLEGIKVTEKFREFAELVAEVLVDKYERIGEDS
TEVSDVIKEAARQHARRTSAKPEPKARNFRSSTGRGKEQETGESDDDDYPEDSD
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Molecule Role :
Protective antigen
- Related Vaccine(s):
VHSV DNA vaccine pCMV-vhsN encoding nucleocapsid protein N and glycoprotein G
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III. Vaccine Information |
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1. VHSV DNA vaccine pCMV-vhsG encoding glycoprotein G |
a. Vaccine Ontology ID: |
VO_0004338 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Zebra fish |
e. Gene Engineering of
Glycoprotein |
- Type:
DNA vaccine construction
- Description:
Vector pcDNA3 expressed glycoprotein G gene of the virus (Heppel et al., 1997).
- Detailed Gene Information: Click here.
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f. Vector: |
pcDNA3 (Heppel et al., 1997) |
g. Immunization Route |
Intramuscular injection (i.m.) |
h.
Rainbow trout Response |
- Vaccine Immune Response Type:
VO_0000286
- Efficacy:
Young rainbow trout injected with the G construct, alone or together with the N construct, were strongly protected against challenge with live virus (Heppel et al., 1997).
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2. VHSV DNA vaccine pCMV-vhsN encoding nucleocapsid protein N and glycoprotein G |
a. Vaccine Ontology ID: |
VO_0004339 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Rainbow trout |
e. Gene Engineering of
nucleocapsid |
- Type:
DNA vaccine construction
- Description:
Vector pcDNA 3 expressed the gene encoding the nucleocapsid protein N (Heppel et al., 1997).
- Detailed Gene Information: Click here.
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f. Gene Engineering of
Glycoprotein |
- Type:
DNA vaccine construction
- Description:
Vector pcDNA3 expressed glycoprotein G gene of the virus (Heppel et al., 1997).
- Detailed Gene Information: Click here.
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g. Vector: |
pcDNA 3 (Heppel et al., 1997) |
h. Immunization Route |
Intramuscular injection (i.m.) |
i.
Rainbow trout Response |
- Vaccine Immune Response Type:
VO_0000286
- Efficacy:
Young rainbow trout injected with the G construct together with the N construct were strongly protected against challenge with live virus (Heppel et al., 1997).
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3. VHSV DNA vaccine pVHS-G encoding glycoproteins |
a. Vaccine Ontology ID: |
VO_0004494 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Fish |
e. Gene Engineering of
Glycoprotein |
- Type:
DNA vaccine construction
- Description:
The pcDNA3.1(+) vector expressed viral glycoproteins (G proteins) (Lorenzen et al., 2002).
- Detailed Gene Information: Click here.
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f. Vector: |
pcDNA3.1(+) vector (Lorenzen et al., 2002) |
g. Immunization Route |
Intramuscular injection (i.m.) |
h.
Rainbow trout Response |
- Vaccine Immune Response Type:
VO_0000286
- Efficacy:
Challenge experiments revealed that immunity established shortly after vaccination was cross-protective between the VHSV and IHNV whereas no increased survival was found upon challenge with bacterial pathogens. Within two months after vaccination, the cross-protection disappeared while the specific immunity to homologous virus remained high (Lorenzen et al., 2002).
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IV. References |
1. Heppel et al., 1997: JOËL HEPPELL, NIELS LORENZEN, NEIL K. ARMSTRONG, TONG WU, ELLEN LORENZEN, KATJA EINER-JENSEN, JOACHIM SCHORR, HEATHER L. DAVIS. Development of DNA vaccines for fish: vector design, intramuscular injection and antigen expression using viral haemorrhagic septicaemia virus genes as model. Fish & Shellfish Immunology. 1997; 8(4); 271-286.
2. Lorenzen et al., 2002: Lorenzen N, Lorenzen E, Einer-Jensen K, LaPatra SE. Immunity induced shortly after DNA vaccination of rainbow trout against rhabdoviruses protects against heterologous virus but not against bacterial pathogens. Developmental and comparative immunology. 2002; 26(2); 173-179. [PubMed: 11696382].
3. Standish et al., 2016: Standish IF, Millard EV, Brenden TO, Faisal M. A DNA vaccine encoding the viral hemorrhagic septicemia virus genotype IVb glycoprotein confers protection in muskellunge (Esox masquinongy), rainbow trout (Oncorhynchus mykiss), brown trout (Salmo trutta), and lake trout (Salvelinus namaycush). Virology journal. 2016; 13(1); 203. [PubMed: 27912771].
4. WISC - VHSV: Viral Hemorrhagic Septicemia Virus [http://www.invasivespecies.wa.gov/priorities/viral_hemorrhagic.shtml]
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