VIOLIN: Vaccine Investigation and Online Information Network

Aeromonas hydrophila

Table of Contents

General Information
1. NCBI Taxonomy ID
2. Introduction
Vaccine Related Pathogen Genes
1. aroA (Virmugen)
Vaccine Related Host Genes
1. CD8A
2. IFN1
3. IgM
4. IL8
5. TNF-alpha
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

2. Introduction

Aeromonas hydrophila, which belongs to the genus Aeromonas, is a rod-shaped, facultative anaerobic bacterium that inhabits aquatic environments. Optimal growth occurs at 28°C. Some strains of A. hydrophila can cause disease in fish, amphibians, and humans (Wiki: Aeromonas Hydrophila).

II. Vaccine Related Pathogen Genes

1. aroA

Gene Name : aroA
Sequence Strain (Species/Organism) : Aeromonas hydrophila subsp. hydrophila ATCC 7966
NCBI Gene ID : 4489425
NCBI Protein GI : 117619998
Locus Tag : AHA_1979
Genbank Accession : CP000462
Protein Accession : YP_856510
Taxonomy ID : 7966
Gene Starting Position : 2172700
Gene Ending Position : 2174067
Gene Strand (Orientation) : +
Protein Name : 3-phosphoshikimate 1-carboxyvinyltransferase
Protein pI : 7.25
Protein Weight : 46562.74
Protein Length : 455
Protein Note : catalyzes the formation of 5-O-(1-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate and 3-phosphoshikimate in tryptophan biosynthesis

DNA Sequence : Show Sequence

>gi|117617447:2172700-2174067 Aeromonas hydrophila subsp. hydrophila ATCC 7966, complete genome
TTTGTGCCACCCGCAGTCTTGCCGAGCAGGGCGCGGGCTGCTAACGTCCGGTCAGGTCATTTTTTTGAGA
TCGTACAGGAAGTCTATGAATTCGTTGCGTTTGGAACCTATTTCTCGTGTGGCCGGTGAGGTCAATCTGC
CCGGCTCCAAGAGCGTGTCCAACCGGGCCCTGCTGCTGGCAGCCCTGGCCCGTGGCACCACCCGGCTCAC
CAACCTGCTCGACAGCGATGACATTCGTCACATGCTGGCGGCGCTGACCCAGCTCGGGGTCAAGTACAAG
CTCTCCGCCGACAAGACCGAGTGCACCGTGCACGGTCTGGGTCGCAGCTTCGCGGTGAAGGAGCCGGTCA
ATCTGTTCCTCGGCAACGCAGGCACCGCCATGCGACCGCTGTGCGCCGCCCTGTGTCTGGGCTCCGGCGA
ATACACCCTGGGCGGGGAACCGCGCATGGAAGAGCGCCCCATCGGCCATCTGGTGGATGCTCTGCGCGAA
GCGGGCGCCCATATCCAGTACCTGAAGAAAGACGGTTATCCGCCGCTGGTGGTGGATGCCAAGGGGCTAT
GGGGCGGCGATGTGCACGTCGACGGCTCTGTCTCCAGCCAGTTCCTGACGGCGTTTTTGATGGCGGCGCC
GATGGCGGCGGGCGATACCCGCATCCACATCAAGGGGGAGCTGGTCTCCAAGCCCTACATCGACATCACC
CTGCACATCATGAAGCAGTTCGGGGTGGTCATCGAGCACGACAACTACAAGCTGTTCTACATCAAGGGCA
ACCAGAGCTACGTCAGCCCGGGTGACTTCCTGGTGGAAGGGGATGCCTCCAGCGCCTCCTACTTCCTCGC
GGCGGGCGCCATCAAGGGCAAGGTGCGGGTTACCGGCATCGGCAAGCACAGCATTCAGGGCGACATCCAC
TTCGCCGACGTGCTGGAGAAGATGGGCGCGCGCATCACCTGGGGCGATGACTTCATCGAGGCCGAGCAGG
CGCCGCTGCACGGCATCGACATGGACATGAACCACATCCCCGATGCGGCCATGACCATCGCCGTGGCCGC
GCTGTTCGCCGAGGGGCCCACCTCGATTCGCAACATCTACAACTGGCGGGTGAAGGAGACCGACCGCCTG
CACGCCATGGCGACCGAGCTGCGCAAGCTGGGCGTCGAGGTGGAGGAGGGGCGTGACTTCATCACCGTTA
CCCCGCCAGCGCAGCTCAAGCATGCGGCCATCGATACCTACAACGACCACCGCATCGCCATGTGCTTCTC
GCTGGTGGCGCTGTCCGATACCGCCGTCACCATCAATGATCCGGGTTGTACCTCCAAGACTTTCCCGGAC
TACTTCGACAAGCTGGCCAGCATCAGTCAGCGCGCGTG

Protein Sequence : Show Sequence

>gi|117619998|ref|YP_856510.1| 3-phosphoshikimate 1-carboxyvinyltransferase [Aeromonas hydrophila subsp. hydrophila ATCC 7966]
MCHPQSCRAGRGLLTSGQVIFLRSYRKSMNSLRLEPISRVAGEVNLPGSKSVSNRALLLAALARGTTRLT
NLLDSDDIRHMLAALTQLGVKYKLSADKTECTVHGLGRSFAVKEPVNLFLGNAGTAMRPLCAALCLGSGE
YTLGGEPRMEERPIGHLVDALREAGAHIQYLKKDGYPPLVVDAKGLWGGDVHVDGSVSSQFLTAFLMAAP
MAAGDTRIHIKGELVSKPYIDITLHIMKQFGVVIEHDNYKLFYIKGNQSYVSPGDFLVEGDASSASYFLA
AGAIKGKVRVTGIGKHSIQGDIHFADVLEKMGARITWGDDFIEAEQAPLHGIDMDMNHIPDAAMTIAVAA
LFAEGPTSIRNIYNWRVKETDRLHAMATELRKLGVEVEEGRDFITVTPPAQLKHAAIDTYNDHRIAMCFS
LVALSDTAVTINDPGCTSKTFPDYFDKLASISQRA

Molecule Role : Virmugen
Molecule Role Annotation : An A. hydrophila aroA mutant is highly attenuated in trout, and a single immunization conferred significant protection from challenge with wild type A. hydrophila (Hernanz et al., 1998).
Related Vaccine(s): Aeromonas hydrophila aroA mutant vaccine

III. Vaccine Related Host Genes

1. CD8A

Gene Name : CD8A
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 925
NCBI Protein GI : 225007536
Genbank Accession : ABBA01075791
Protein Accession : NP_001139345
Taxonomy ID : 9606
Chromosome No : 2
Gene Starting Position : 86784604
Gene Ending Position : 86808395
Gene Strand (Orientation) : -
Protein Name : transcript variant 3
Protein pI : 9.86
Protein Weight : 24339.21
Protein Length : 235
Protein Note : Also known as CD8; MAL; p32; Leu2

DNA Sequence : Show Sequence

>gi|568815596:86784604-86808395 Homo sapiens chromosome 2, GRCh38 Primary Assembly
ATCGGATGCTGTTTACCCATTAAAGAGGATGCTACAAATGTTTATTCATTAAAGCCACTCATAACAGCAT
AGTACAACCCTATTTAAAAAAGAAAGACATATTTTACATGTATGTGTAGAAAATAATCAGGAAGAATGTA
CACCATATTAACAGGAGATTATTCCTAAGGTGAAGAATTATGAGTGGTTCTTATTTCCTTATCTACTTAA
GTTTTTTCTCTCCAATGGGCAGGCATTGCTTGTACCATACCTTAAGCAAGGAAGTGCATGTTTGGGACAG
CAGTTTGGGCTCTCAGCCTCCTTAAGAGAGTCAGGTCTGCCTCATCCCTGTATCTGCTAGTTGAGCAGAG
GCCAGGGCTGTGTGAGGGGCTCTCCAACAATTGTCTTTACAAAGTATAAAAAGTCATCAGTGATCCCAGG
AACATGTTTGTATCTCAAATTCAGAGATTCAAGAGGGCCTTAGTTTAACCTCACTGATGCTCAAATTCTA
TTTGTAAAGGGGTAGCCTGTCCTCTTTCATGGGCCCCTCTGCAATGCAAGGGCTGGGAGAGCAATTCCGC
CTCCACATAGGGGTTTCACAGAGATTTTCTTTAGAGATAGAGGGATTCATTTTCCAGGGTTAAGCTCACC
ACTTCATTTTATTTTAGGTCTATACAATTTTAGGCTTGATTATAAAAAAAAAAAGTCTGATATTGTTTAC
ATTATAGAACTCTGCCAAAGGCAGTTCTCTTCTTTAATTCATTACCTCCTCGAGGCTCTGGGCACAGTAT
CCCAGGTATCAAGAAGTACTTGTTCCCTTGCCGTTGGAGACTCAAGCACCTCACCCTGAGACAGGGGCCT
CGGAAAGAAAGACCTGAATGGTGTGGAGGAAAGAGCCCTGAGCTGGGAGACAAGGTCCCTCCAGCTACTG
CTCCAACCCTGACTTGCTGTGTGCCTTTGATCAAGCTGTCTCTGGGCTTTAGCCTCCCCCTTTGTAAAAC
GGGCGGGGAAGAGGTTGAGATGGCATGGGTGCCTCCAGCTCTCTCAGCATGATTCTGAGAACTCTGCGGG
TAGCTCTGGCCTGCCCCTTTCCACGCCCTACCGCGATGTGCGCACAACAGTATTGTGACCCTTGTGGTGT
ACTGTAGATTTTACCTAGTTTTGTTTCCCGTCAAACACATAAAGAAAAAGTAATCTTTCCCACCCCGCCC
CCACTAAAATAATAATCATGAGAATGAATACACAGGGAGGAAGACTGGAAAAAATGAAAGGGAAGGACTT
GCTCCCTCAAAAGGAAGGATCTCAGTTTGAAGTAATGTAGTGGCTGTTGCACAGGGTTAGACGTATCTCG
CCGAAAGGCTGGGCTTGTCTCCCGATTTGACCACAGGCCTGAAAGAGAGGAAAGCGACCATCATTGTAGC
CAGAACCCCGCCAGTGCAGCATCCATCCAGCCACGTCCAGCCTCCCAATCCCCCAGCCTTTGAAAGGTCT
GAGAACCTGTTCCTGGGGCACCAGCATGGGACTGGGTGAATGACGTCAGCACAGCACCCGTGCTAAATGC
CTTACTTGCACCATCTCATGTAATCCTCACAGCAGCCTTAAGGGTGGGCACTGAGATTGAGAAGTTAAAC
TTGCCCAAGGTCACACATCTAGGAGGTGGCTGAGCCAGGATCTAAACCGAGTTAGTCTAGCTTCCTGGAG
CCCCTGTCAAGGTCTCCACAGAGGTGTGAGAGTGAGACTGATAACCAAAGTCATTAGCTGACCTGATTCT
CAGCCCCGGAGGATGACAGGGAGAGAGGAGGATGTGAGCAAATCACCACCATCAGCCAAATGATGTTACG
CTAAAAACGTGTTAATTCAGCACCAGGCTAGCACCTTGTAAACATGCTAATTCATCATGAGCGTCATGTC
TGCACTCTCAGCCCTCTAAAACCTCTCTGTTGAAGCTGAGCGGCCCACCATCATCACTGAGTATATTAAC
TCGGGTGTTGGAATTTCCCAGGTGTGTTTAATCTGTATAGCTTTGTATTTTAAGGCAGAACTTTATTCTC
AGTTATTCATCTCAAAATGAGAGTTTTCAGAAAAGATCGAGAAAAGGGATGGTCCTTCTGCAAAAGTTCC
AAAGTGCACCCTGAATAATAAAAATGCTAAAAACTGGCCGGGTGCGGTAGCTCACGCCTATAATCCCAGC
ACTTTGGGAGGCCAAGGCGGGCGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAA
CCCCGTCTCTACTAAAAATACAAAAAATTAGCCGGGTGTTGTGGCGGGTGCCTGTAGTCCCAGCTACTTG
GGAGGTTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCGCACCACTG
CACTCAAGCCTGGGCGACATAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAGAAAAGAAAAGAA
AAGAAAAGCTAAAAACTTCCCCCCCCACTTTTTTTTTTTTTTTTTGTGAGATGGAATCTTGCTCTGTTAC
CCAGGCTGGAGTGCAGTGGTACGATCTCAGCTCACTGCAACCTCCGCCTCCCACGTTCAAGCGATTCTCC
TGCCTCAGCCTCCTGAGTAGCCCGGATTATAGGCATGCACCACAATACCCGGCTCATTTTCAAAGTCTTT
ATCCCCTTGGTATATCACCTGTAACATATGCATTGGAAATCCATTGCATTTCCATTGCTAAAAACAACAA
TGTGTAAAGTCCTGAACTTGGATTCCCTCAGAAAAATGTAAAGGAGTATAAAATGCAAACTCCAGGTATA
AAATGTAAAATATTTCATCTACTGAAAAGAACTGGTGTACACAGTCGGTTATCCTTAACACTTCAGCCTG
CAGCCATGGGGAAGGTGTCTGAGCTTTGGATGGAAGTTGCAGCTCCCGGGCAGTATGACCTTGGACAAAT
CACTAAATGTTCTAAGCCTCAGTTTCCTTATCTGTAAAATGCAGGTAATCATGACTACTTTAAAAGTTGT
CATAAAACTTAAAATTAAAAAAAATCTATAAAGCAAGTAACGTGCGTTGTAGGCTTTCATCAAATGGTTA
TTACTATTGATAATCTGAGTTTAATGTGCCTTTGCCACTTTACTAATCCCTGAGCCATGAGATGGCACAA
AGTTGCTGTGAGGATTAAGAGAGATGGGGAGTATTACCCCTATCACAGTGCCTGACTAGAGCAGGCACTT
GTTAGATGCTACCACAGGGATGCCTCAGCCAACGGGTGGAGAGAGGTTTTTAACAGAGAGGGAGAGAGAG
AGAGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATCATGGGCTGAAACTGGATGAGGCAGTCACTTGAG
CTGCCAGATAGCCAATTGTCCCTGCATCTCGTTCCCCTTTTTTCTACTCCAGTCCTTGGCAGCAAACCAA
GGTCCTACATGACACAGAGAAGTTAGCAACCAAAGCCATTATTATTTTGGTTCAGCTGATAAAAGATTGG
CTCTCCCTCAAAAAACTAGACCAGCCAAGTAGGAAAAAAAATTGTTTTCTTAAGAAAACAACAAAACCCA
GATGGAATAATCAGAATAAAGAGGAGGTCAGGTCCTACGAGGAGCTAGGATAAATTGGGGGAATCCCTCA
GGTTTTTTTTTTTTTTTTTTGGAAGTGCCAGGTCAAATAAGATGGAGATTTACTGGAGTCACACTGCTGT
TGGCACCCGCCTAACCCGGGCCTCCTTTTTCTCAAAGACACAGGCCAGATGCCAGCCTTGGCCTCTCTTT
GCTCAGGAACTGGCTGACTCCCTGCCCCAGGATTTCACGGCCATGACCTCTCTCTGGGAAAAGTTCCCAC
TATTTGCTGCTGGGGAAACCAGGACCCCACCCCAGGGCTGGCCAAGGTGAGCAGGCTGAGTTCAAAAGAG
ACTCACCGGGGACATTTGCAAACACGTCTTCGGTTCCCTGGATAAGGAAAAAGAAGGGAAAAAGTGAGTG
CCCCTATCCATCAATAGTCCCCAAAGACAGTGTATTTTTTGTTGTTGCTGTTGTTGTTGCTGCTGTTTTT
TGGTTTTACAAAAAATCATTTCAGAACGATCATGGGCTGAAATTGGATGAGGCAAATAGGGAATTGCCTC
TACGAATTCTCCTTCCTTTCCCCTATTTCTTTGAGGATTATAACTCTTGAGAGCCGGGTAAGGGCTTCTT
ATCCATCCTTCGTCTCAAGCATCAATTAAAAAAAATTGCAACTACAGACGCGGGCGAAAGCGGGGAGCCT
GCAGACCTGATCCGGCACCTCTCTTGCATGGGACCCAAAGCAGGGTGGGCGGTTCGCCGAAGAGGGCCAA
GGACGAACCTCAAACTGATGGTGGTTTTGACTAACCCTCCTGGGCACTTGACTCGGCCACAGCGGCCCCC
TCCCGGCGGGCGTTTGTCATGTGTCCAGACATGTGCGCGCGCGCTGCGGGCTTCGTCGACGGAACACACC
CGCTGCAGCGGCTCTGGGAACGCGGCTTTGTCTCCCAAAACCTCTCCGAGAGCGCAAGGAGCGGGAAGGG
CTTTCTCGCATTCTATCTCCCAGAAAGAAGTGCCTGTACCTGCGGGGCAGCTCGGGAGTCCCAGAAAACT
CAACCCCAAGCTCCCCCCTCGCAAGGTCCGCCTGGAGCTAGCAGAGCCAAGGGCAGGAGCGGGGCCGACT
CCTTCCCGCCGCGATTCCTCGGGACTTACTGTGGTTGCAGTAAAGGGTGATAACCAGTGACAGGAGAAGG
ACCCCACAAGTCCCGGCCAAGGGCGCCCAGATGTAGATATCACAGGCGAAGTCCAGCCCCCTCGTGTGCA
CTGACGACACCAAAGACGCCGACATTTAGGAGAGGGCCCGGGACCTCCCAACCGCCCCACCGTCCCGGGA
ACGTCTCTCCGCCTCAGATCTCGGTTTCCCACCACTTGGACAGCCCTTGACTCTACCTACAGTATCAGGG
CTGTCCCTGGCATGGGCTCTCCCCGCGGTGCGTGCCGCCCCCGCCCCGGGCCCCCGCACGCCTCACCTGC
GCCCCCCGCCGCTGGCCGGCACGCCTCTGGGCGCAGGGACAGGGGCTGCGACGCGATGGTGGGCGCCGGT
GTTGGTGGTCGCGGCGCTGGCGTCGTGGTGGGCTTCGCTGCAAGAGCAACAGAGCGTGGTTGGGGGCCAG
GCTGGGGTTATGGAGGCGCCCCAGCCCCGGCCTCGCGCACCTTTCCCCACGGGGACGCCTCCCCCCGGTT
TTCCTGGGAGAAGGGATAGCAGAGGAGACAGGATGGGGACCCCGGGATGCGCGCGGACCCCTGTGCTCGG
GCCTCGGCTCAGCCCAGCGCACCCGGCGCCCAGAGCCGGAGCGCAGGAGCCAGCTCCCCTGCACCTGGCC
TCTCTCCCGGGCTGAACCAGCAACCCTGGAGCGCGGGTTGATTGCTGTCCGCATTTTACCCACGAGCAAA
CGGAGGCGCAGATAATTTAAGTCATTTGCCCAGAATCACACAAGAATCCGAACTCGAGTCCAGATCGGCC
GGACGTAAAAAGCCATCCCCAGTCCCTACTCAGCCTCGCGTTAGCCTCAGTTTGCTGTGTCTGTGAAATG
GGAACAGTATCTAAGTCGCTTCCAGGTGCGCTAAGAGGCTTGAAAGCAGGGCCCAGGTGTGGAAAACAGG
TTGAGGTGAACCCCAAGCCCCACGCGGAGAGGTGCCGCAACCCGGCGCGCGGACCTGGCAGGAAGACCGG
CACGAAGTGGCTGAAGTACATGATGGAGTTGCTCAGGGCCGAGCAGAAATAGTAGCCCTCGTTCTCTCGG
CGGAAGTCGCTCAGGGTGAGGACGAAGGTGTCCCCCAACCTCTTGCCCGAGAACCGCTGGGTGTCCAGCC
CCTCGGCCGCCTTGGGCTTGTTTTGGGAGAGGTATAGGAGGAAGGTGGGACTGGCGGCGGCGCCGCGCGG
CTGGAAGAGCCACGAGCAGCCCGACGTCGGGTTGGACAGCAGCACCTGGCACTTCAGCTCCACTGTCTCG
CCCAGGTTCCAGGTCCGATCCAGCGGCGACACCCGGAACTGGCTCGGCCTGGCGGCGTCTGCAGGCGGCA
AGCAGCGAGGCTGAGCCCGCAGTCCCGCGCCCCCCGCCCCCCGCCCGCCCCATCCCCTGCCTTCCCGGGC
GTCTCAAACTCACGGAGCAGCAAGGCCAGCGGCAGGAGCAAGGCGGTCACTGGTAAGGCCATGACGCGCT
CCCCAGGACGCTGCTTGGCTCGAAGCTCGGGCGCGAGGGGAGGCGCGCGGGAGCCGGTGGGGCGCCGAGG
GGGGAAAGTTGCGCCCTTCGGCCGGCCCGGAGCCTGATTTCGCATTTGGAGGATGTGATGTCACCCGAAG
CCCCCGCCGAGGAGAGTCACCCTCCTTTTCGCGGTTGTCGCCTTCCAGCCCGGCGAGGAGGCTGGGGCCC
GTGAATAGGGCCGTCGAGGCAGCCTGGCCAGGCAACTGGGGGCAGCTGAAAACTGCGGGTTTGGGGATGA
GGAAAAGGGCTTGGAAATAGTCCTTGGAAATGGTTGTCTTGTGAGAGTGACAGAGTGGGTGAAGGGAGAC
CAAAGATTTCAAGAAGTGAGGGCGAGAGTAGGCAGCAAAGGAGGGGAGTGTCCCTTCCTTTGCCTTCACT
AAAGGCGTCTCTTGTACTGTCACCTTGGGACTTTTTATTGGCAAAATGGGCACTGAGGGCTGAAAAGGAA
GAGGAACTAGCGACCTGCCCGCTTCTGAGGAACTCGCTAGAGCAGCCCCAGTTTTCACCGAGGAAGGACC
CTCTCCCTTCCCCCAGGAGATTTCCATGAGAGCGGCAGCAGCCGAAGCTTTGGGTGTCGGTGTCAGTGCG
CTGCTGACCTCATTCTTCCGGCCTTTCATCCAGCGGCTAAACTCACCACACAGCCTCATCTCTTCTTGGA
GCCATTGCAACAGCCTTCAATTCACACCAGTCTCTCTCCTGATCAGTCCTCCAGCCACGTTGTTATAAAA
ATTATTATTCTCACAAAGGGGATCTGACAGCGTCACTGCTGCAGCCTCCCATGGCCTGCATTGCTGGATG
GAAATTCAACCCCCAGCTTGGTTGACCAGCCCTTGGGTGCTGGCTCCTCCCAGCCTCCGCACAGCCCCCT
GCCCTGCCTTTCCCATACACCTGCAGCTGCAGCTTCACAGGCTTGAAGTCATTCAACCCCTGCGCTCTGT
GCTTTCACGCCCTTGCACTTGCGGTCCCTCGGCCTGGAATGCTGGTCTGTTTAAGCCCTGGAGGCAGCTC
CTGTGCTGTCTCATTTGGACCTCATTTCTTTCACCCCCAGCTCTGGGTGCTCTGCAGGGAGGTGTGGGTG
ATGTGGGTGAATGCTTGGGTAGAGGCGGTTTTCTCTTTTCTGTCCCTAACACATGTGTGTCCCCTTCCTT
TTTGTTTCTTTAAAAAATAAAATAAAATAAAAAAGACTGGAGTCTCGCTGTTTTGCTCAGGCTGGCCTTG
GGACTCCTGAGCTCCAGTGATCCTCCCACCCCAGCCCCCTGAGTAGCTGGGACTACAGGCTCATCTTTCC
TTTTTGACTGTTGCTGTCCTGAAACCTTCCAGCTCCAGTGGCATGCCCTCTTCCCATTTCTTCAGGGTTT
CTGAGTCTTTCCACTTTGAATCCCATAGTGGCTGCACCTCACATTGCTACTGCTTGATCCTAGAGACAAG
ACACCTGGCATTCTGTGTTCCATGTGTCTGCCCCACCCATTAGTTCTGCTGGGCGAGGACCATGCCGAGA
CTTCATGATGTGTCCTCCATCCCCAGAAACACAGCATGGGCTGAGCTGTGCTTGTTGAACTGCATTCTCC
TTGTCCTTGTGAAGTACAAACTTAAGTGTTGGATGGGTTCTTTGTCGTTGTTGCTATTTTTTTTTTTGAG
ATGGGGTCTGGCTGTGTTGCCCAGGCTGGAGTGCAGTGTGGCACCACCTCGGCTCACTGCAGCCTCTACC
TCCCAGGCTCAAGTGATCCTTCCACCTCAGCCTCCCAAGTAGCTGGGACCACAGGAGCACACCACCATGC
CCAGCTAATTTTTTGTATTTTTGGTAGAGATGGGGTTTCACCATGTTGCCCAGGCTGGTCTCCAATGCCC
GAGGTCAGGCGCTTTGCCCCGCCTTGGCCTCCAAAGTGTTGAGATTACAGGTGTGAGCCACCATATCAGG
GCCTGGATGGGCTTTTTTTTTTTTTTTTAATTATTTTGATTTCAGTACCTCCTTCCAGCTCCCTCAAAGC
ATCCAAGATGCTTATTCTAGCATGGAACATGTAACATTTTATATTCAGAAATTTTTAAAAATGTCTTTTA
AGTGACAACAGTGCAATATATTCTTGTACGAAAACAAACACAAAGTTCCCTGTCTCAACTCTCCTCACTG
TCACCTGCTCCCCACAGAAAACCACTGTTACAGTTGAATGTGTCCCTTAGATATTATCTGTACATAAACA
GATGTTTATACATCATCTTAGACACATGGGATTGTATGAGATAAATATTTTTTTGCAACCTATCTTGTTT
CACAGTAGAAGGAGAGCGTTCAGCAGATGAAGATCCACCCCATTCTTATAAATGGGTGCATTATATATGG
GTGTTCCATAATTTAATTCTGCTGTTAGTGGACATTTAGGCGATTTCTCATTATTGCAACACTAGCAAGG
CTGTGGTAGATATAATGTATCTGTATCACTGAGGGCTTGCACCAGTACCTGCAGCTTTTTCAAATGGGAG
ATGAGGGAGTTGGGGAAGAAAACACCTTTACAATGATGGTTATAACTGGCCTATCGATGTATGCATGTGT
GTGTGTGTGTGCATGGTATGAGGGTCCCTCGCTGTGTAGACACAGTGCTGTCCCTCAACTCACATGAATG
AGGGAGACAAGGAAGCAATCAAGTCCAGACAGGCCACCAACACATCAATTCTTGCTGTAGATGGTAGTTT
GCCATTTTGCTCAGCACCACCAGCACTGTATCAGGAGTGTCAAATGGCCCTGCTGGCCAAAGGCTACAAT
CAACAGGGTGGTAAGGAAGATGGCTAGACACGAAACTGCACTTGCCCAGTGGTGGAAGCACTGAGGGGGC
CAGAGTCAACCTCCACCTATTTGGATCTTGAGAATCAGGCCCCTGAGTGTGATGATGGCCAGATTTGGGG
GTGTACTGCTTTAAAATAGACTAGTTCCATCTTAGTCTCACGGAAATCAGCTTGGGGGCCTTCTAGCCCT
GCAGCTCAGAAAAGTGTCAGCCAGTGGGGTGGCAGCCCCTTTGTACAGAGCACCATGGGGGTTGGGGTGG
GGAGGATAAGGCAACATGTCAAACCCATCAAGGAGGCTTTGTGACCCCAGTGATATTTTTTGCAGAACGT
GGTGGATTTTCGATGTGACCACAATGACATCCGCCTTGCAGTGGCAGAACAGATGCAATTGCACAAGTTC
TGGAGAAACTTTCTGTAGACCAGGTGTGCAGAGGGCTGGGTTGGGCCTGTCCATCACTGCCAGGTAAGTT
CCCAAACCCCACACTGTAGCACTGACTTGGGATGGCATGAAGAAATGTGAATGACTGCACTCCTTTAAGC
AAGCCCCTTATTTAAAAAAATATCCAGAAGTAGATAGAGTAAGATAGTAACCAGTTTCTATGCTGATTTA
AACTGGTCCATGGAAATGGAAAACTTTAATGTAGATTAAAGACATATTCAAAATGCAAAACCGGGCAGAG
CAGTGGTAGGAAATCCATCAGGGTTCTGTGCCACTGCTCCTAAGACATGAACTGCAGGGACAACTTGGAA
TCATGGCTGGGAAATTGCCTGCTGTGTCCCCATCGCCAATTCAGGTCTATGGAGATGTTACTGTTCTTGG
AAGGAGTTAGCTTGTCCAGGGCTAGAAACTCTAGAGTTATCAGGGCTTGTTTTATCTTAATTGGAAATAC
CAATCAATTTGGCCTCACTTCTGTCACCTTTCTTTTCCTCCACCCCGAGTTCCCCAAGTTCATCTAGCTG
CTGTCCTTCTTTCCTGGACTTTTGCAATGGCCTCTTAACTGGCCAACCCCATTTCTGCTCTGGTCCCTTC
TAGAGAAAATCTCATCATGCCAACCCCCTGCTTCAACCTGAAAGGACTTCCTGGTGTCCCTAGTAGAATG
TCGGGGATCCCTAATGTTGCCTGTGAGGCCCTGCACACTTGGTTTCATAGTCTCTGTCCTCTTGTCTGTC
ACCTTCTTCACTCCTCCTAGGTTCCGCATCTCTCATTTCTCAAGCGGACCCTAGTCCTTTCCACTTCAGA
GCCATTAAACATGCTATTCCCTCAGCCAAGAATGTTCTCACCTCTGCTCTCTGCTTACCAAACTCCTCTC
CCTTAAGACCCAGCTCAAGCATCTCCTTCCTGTGGGGTTTAGCTCCTTCCCCCTCCCACTCCCAGACAGT
ACAGACCACATCCTTCTCTTCTCTGTGTCACCCGGACCTTGGGTATCTGCAGACTGGAGCACAATGGTTT
GTTTGCCTGTCCAGCTTGACAACCAAACCCTTGGTCCCTTGAGGAAGGAGCCTTGTCCCTCTCATGTTTA
CACCAGGGTGCCAAACAGAGGGCTTGGCACACACTTGAGTGGCACACACTCAAGAAATTCTTGCTCAGTA
AAAGCTAAAGGAAGGTAGAAGTAGGGTACAGCAGACCAGAGGAGGCTTAGATAGTTCATTCCAAGTAAGA
GTGGTCAGGAAAGGCTTCACATATAGTTGGGTTTGAAGATAAAGGAAGAATTAGGAGAGAGGTTGTTTAG
AATGAGGCATTTGATATGTCTGATCAAGATCAGCTGATACCATGCTTTAACATATAGCAATGGTATAGTT
AAAAACAAAAGAAGAGGAGGAAGGGGAGAAGGCAGATGTACTCAAAAGCAAGATAAACGTCTTCAAGGAT
GTAGTTGCAGGACAATGGCTGGAATCAGGGGCTGGCTTGCTGGGATCTGTTCTTAGCAAACCATTCTTGG
GCCTGGGTCTTGAGGGTCTGTTCTTAGTGTAGAGGCACCAAGAGTTTGACTCCTTCAGCTAACAGAGTTT
CACAGTGCTTTGCCTATAGAGGAAGGCTGGAGCCAGGCTCTCTGCTTGAAACAGGGATTTGGATCTGATT
TTCTCTTGATTGAAAGAGAGCCCACAGCCACCCATGTAAGGCCTAGTTCTGACTGTGGGCCCTGGAAAGT
CAGAGGCTACTTTAAAAAACTCAGGAAAAGAGAGAGTAAAGAGAGGAAGAGATTTTCAAAAATTTCTATT
CAAGATAACCCTGCACACACACTCGAAATTCAAAGTATACATAAAAAAGACTCAACAAGTGGAATGTGAA
TTCACTTCCAAAAAGAGGCAAATTATTGAACAATTTGAAAAATGTTTAAAACAAAGAGAACAACAAATGT
AGAAAATGCAGAAATTAAAGAAGAACAGTTGAAGATGATAAATACTGAGCATACTGGAAATGAACACTCT
ACATATTAAACAAGGATATACTCTAGGTTGGACATAGTTGAAGGGATAATTAATGAATTGGAAGCTAGCA
TTGAGAAATTCATAGAGAATGCAACACAGGGAGATGAAGACAGAAGCAGTTTGAAAAAGCTGTTGAGATG
TATATTAGCATGGTGAAGTGTTTAATAGGATTTCAGAAAGGGGAGAACAAAGGGAATGTGGAGAAGAAAT
ATGGGAAGAAAGCATGGCTGAAAATTTTTCACAATTAAAGACAAACATTCTCAGATCAAAAATACTCTTA
GTGCCAAGTATAAAAACATATACATCTTTGCACATATCAAGGTGAATTATAAACATCAAGAGTAAAGAGA
AGAGGGCATCCCAGGAAGCTAGGATGTTTGAAATATAATTGGGAGAATCCTTGTTGCAGTTTGGATTGGG
CAGAGAGGGTAAGAAGCTGAACTGTAATGGGGAAAAATATACACATTTTGGTTACACCTTGCATTGACTC
TCTGTCATCCCCTCCCTTGGTGCTGACACATGCTTACTGTAAAACTGATCCAATGATCCCATAGAGTTGA
TGTTTGTGGTTTCTTTGAATAAACATATAAATTGATCCTTGCCTTCTTAAAACCTGAGAAAGTTACATTT
GTCTTATCTGAGTTCCTTTCTAGGGAAACCAACTATCAGGCCTCCCAGATGGTAGCAATGAGCTGAAACT
CACCAGATCACTTATTATGAACAATAAGACGTCAGACTCTTCACCTGGTATGATGGCCTAACTAACCTCC
TGTTTCCTGTTGTCCAACTCCTTTTCCTTACTCCTCCCAACTTCCTGTTTTCCCACACATGATTACATGT
CTTCCCTGCTATATAAACCCCTTAATTTTAGTCAGGTCAGAGGGATGGATTTGAGACTGATCTCCTATCT
CCTTGGCTGCAGCACCTGACTAAATTCTTCTTCCTTGGCAATACTTGTTGTCTCAGTGATTGGCTTTCTG
TGAGGTGAGCAGAAGGACCTAGATGGATCCCCCGGTGTTTCGGTAACAAAATTGTTTTGGATCTTTAATC
TCAGTAACACTGAAACAGAAGCGTTCTGAATGCAACTGGAGAGCCAGGATTTGAGTTCAGAAGAGGGGTC
AGGGCTGGGGATGTAGCCTTGGGAAGGAGGTGATAAGTTGTGAGAGTGGGTGTCATTGCAAAGGGAGGTG
GTGTGGAAAGAACATACAGAAGAGTATATGGAACCTTCAGGGAAGGCTCGTATTGAAGAGCAGGAAATAA
AACATTGGCAAGGAATCCAGGTAGTGCAGGAAATTCAGAATGGGGCTTGAGAAGGTGGAAGAACTTCACA
GGGGAGGTTTGGGCTATAAGAGAAGCACAGAGAGGACATCAAAGCCTGTGGCAGGTAGAGATTGTAAGCT
CATGTCTGCCCCTGACCCACATGAGGATGTCCCACATGTTTGCCATTGGTGGATGATAAGATGAGGAGGA
CTCACAGGAGAAAGGCCAACAAGGGAGGGTAGGGGCAGCATGGGGACTTGTGGAGGGCAATGTCAGATGA
AGTGATGTTGACTTCTGAACTTCATTCATTATTCACTCATTTAAATATTTCTGCTATTCACTTTTTTAAA
AAAAAGTTTTGAAAACATTTTAACATAGAACAATATGTCAAATAATATAAAATGCCCACTCTTTATAAGT
GTTAGCATTTTGTCGCATTTCCTTCACATGAACATTACAGATAAAACTGTACCCTCCCAAGTACCACCCC
ACCCGCCTTCTCATGAAAAGCTAAATTTGGAGTTTTGTTCATTAATTATGACAGAAAGATGGTAAGTTTT
TTAATTTTTAATCTGTTAACCTTTCCTATGGAGCTGAGAGAGAAGGATGCCGAATGTCCAATGAGTGTGG
ATTTGTCTATTTCTCCTTTTAATTCAGTTCATATTTTGTCTCCTATATTTTGAAGCTTTGTTATTAGGTG
CATACACATTTAGAATTGTGCCTTCTGGATGAATTCACTCTTTTATCATTATGAAATGTTCGTCTTTACC
TCTGCCTCTGGTAATACTTTGTTGTGAAGTCTACCTTTTATTCATAAGGAACAGAGCTTTCTTTCTTTTT
TTTTTTTTTGAGATGGAGTTTCCCTCTTGTTGCTCAGGTTGGAGTGCAATGGCACGATGTTGGCTCACCG
CAACCTCCACCTCCCAGGTTCAAGCTATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATG
CACCACCACGTCCGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTTCTCCATGTTGGTCAGGCTGGTCT
CGAACTCCCAACCTCAGGTGATCCACCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCAC
TGTGCCCGGCCAGAAACACAACTTTCTTATGCTTCCCGTTTGCATAATGTATCTTTTCCTTTTTCTTTTT
TTTTTTTGAGGCAGAGTCTAGCTCTGTTGCCCAGGCTGCAGTGCAGTGGTGCGATCTCATCTCACTGCAA
CCTTTGCCTCCAGGGTTCAAGCAATTCTCCTGTCTCAGCCTCCTGAGTAGCTAGGACTACAGGTGCACGG
CACCATGCCCAGCTAATTTTTATATTTTTAGTAGAGATGGGGTTTCACTATGTTGGCCAGGCTGGTTTTG
AACTCCTGACCTCAGGCAATCTGCCCACCTGGCCTTCCAAAGTGCTGGGATTACAGGCATGAACCACTGT
GCCCAGCCTGCATAATGTATCTTTTTCTATCCATTTACTTTCAACTTCCCCTTCTCTGTTTTGAAATGTT
TACTAGATATGTATACATGTATATGTGTATATATATCTATAAATGCTATATAATTTTATTTTCTGAGAGT
TTCAAAATACAGAAATGTCATCATATTGTGTGTCTTTGAGCTATGTTGATACATATAGATTTATTTTAAA
TGCTGTGTGGTGTATTATCCTGTGAATGTGCCACAGTTTCATGTTCAGTGGTTCTTGAACCAGAGTGTTC
ATCTCTCTGGAAGTATATGGAGGCTGTCCTTGGGGATACACATAATTTTAAGGAAATTGTTTTCCAGATT
CTCAACTCCCATTGGTTCTCTTCTCATAAAATGGACCTGCCCAAGAACAAGTCCCTGTGTTTAGGCTTTG
TGCTCGTTCTCCTTTCTGGCCTATTCTTTCATGATTATTTTATTTCCCACTTTAAAACTGAAAGGCAAAC
TTCCCGCCCTCCTGTTCCTTACTATGCTCCAGAGGTGGGCACGTAATCCTCTGGGCCACCAATAAAAAGA
CAAATCCAAACATTTTTCATGTAATTTCCATCCGTTAGAAATTGCCAAAGAATGCATTTCTCCTGAGCAG
AGTCCCCAGTATCCAGGTAGAGTCATCTGGGGGCCGGGCACGGTGGCTCACGCTTGTAATCCCAGCACTT
TGGAAGGCCGAGGTGGGCGGATCACGAAGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCC
ATCTCTACCAAAAATACAAAAAATTAGCCGGGCACGGTGGCGGGCGCCTGTAGTCCCAGCGACTCGGGAG
GCTGCGGCAGGAGAATGGCTTGAACCCGGGAAGTGGAGCTTCCAGTGAGCCAAGATCACACCACTGCACT
CCAGCCTGGGCGACAGAGCGAGACTCTGTCTCAAAAAAAAAAGAAAGAAAAGAGTCATCTGGGAGGGATG
CAGGAGGCAGAGTGCCTCACAGCAGGCTTTGGGGCCTCATCCCTCTACTCAGCTCTCTATCATCGAGCTA
GAATTCAGAGGTGAGACAGTCATTATGAGGATGCATATTAACGTGTTCATTATGATTACAAAAGGAATCA
GAATTCTTTTTTTTTTTTTTAACAGAAATTAAGTCTGAGTTGTTAATTGGTTTGACATTGAAGACCAGCT
TTGCCAATGAGGTTAATGGCAGTATTTTAACTTTCCTTGAATCTAGTGAGCTAAATCTTTAGCTCCAAGG
TTTTGATGAGAATCAAGTTTAGCGCTAAAGAAACAGCTTAGAAAAAGAAAGAAAAGAAAACACTTTTAAC
AAGTTTAAAATCTATGATAAGATAAAAGCATTTTGGCCAGGTGCGGTGGCTCATGCCTGTAATCCCAGCA
CTCTGGGAGGCCGCAGTGGGTGGATCACAAGGTCAGGAGTTTGAGACTAGCCTGGCCAACATGGTGAAAC
CCCGTCTCTACTAAAAATACAAAGAAAATTTAGTCGGGCATGGTGGCAAGCGCCTGTAATCCCAGCTACT
CAGGAGGCTGAGGCAGGAGAATTGCTTGAACCCAGGAGGTGGAGGTAGCAGTGAGCCGAGATCACACCAC
TGCACTCCAGCCTGGGCGACAGAAAAAAAAAAAAAGAAAGAAAAAAAAAGATAAAAACATTATATCACAA
TATTGTTTGTATTGGAATTTGTCTTGAAATTAAATTAAAAATATTTCTAATACCCCAACACTTTCCAAGT
TAATCAATTTCAGCAATCGTTTTTAAGTGAAAGAAGAATGATGTCATTGATGGCTACTTGGTAGGCTTCG
GTAAAACCTAGTTTAGGGCTTCCAAAACCCAAGAAAGTGAATAAATCTAAAAATGGTAACAAACCCATGT
GCAGGTCAGTGGTTCCCAAGTGTTTGCTTTCAACAAAATTGGAAGAAGGCCTTCTAGATTTGGCAACTAT
CACAGGTTGAATTGTGTCCCCCCAAGTATATTTGTTGAAGTCTTATACTCTAGCATCTCAGAATGTGACC
TTATTTGGAGACAAGGTCCTTACAGAGGTAATCAAGTTCAAATGAGGCCAATAGGATGGGTCCTAATCCA
ATATAACTGGTGTCCTTGTGGAAAGGGGCAATCAGGACACACACACAGAGAACATCATCTGAAAACAAGG
CAGAGATGGGGTGATGTTTCTACAAGTCAAGGAATGCCAAAGATTGCCAGCAACTGACTAGAAGCTAGGT
GAGAAGCATGGAACAAATTCCTTCTGTAGCCCTCAGAAGGAACACACCCAGCTGACACCTTGATCCTGAA
CTTCTAGCTTCTAGAACTGTGAGGCAATAAATTTCTTTTGTTGAAGCCACCTAGTTTGTGGTACTTTGTT
AAAACAGCCCTGGCAAACTAATACAGCAACTAATAGATCATTAAAATTAATTTTTGATGATAGATAACAT
TGTCTCCCTCCCTCCCTGCCTCCCTCCCTTCCTTCCTTCCTTCCCTCCTTCCCGCTCTCTTTCTCTCTCT
CTTTTTCCCTTTCTCCCTTTCTCTCTCTCTCTCTCTTTCTCCCTTGGAGAAAGGGTCTTGCACTTTCGCC
CAGGCTGGAGTGCAGTGGAGTCACTGCAGCCTCAACCTCCTGGGCTCAAACAGTCCTCCCACCTCAGCCT
CCCAAGTAGCTGGGACTATGGGTGCACACCATCATACCTGGCTAAATTTTTGTAGAGATGGGGGTTCACC
ATGTTGCCCAGGCTGGTCTTGAACTCCTGGACTCAAGGAATCCATCCATCCACCTTGGCCTCCAAAATGC
TGGGATTACAGACGTGAGCCACTGCACCTGGTTCATGCTTTTGTCTTTTTGAAAAAAAAAGGAAAAAGTT
AAAAGATTTAGTGATTCTGCTGTAACAAAGCCAACTATTTATTTATGGAGACAAAATTTCTCAGTACTTA
CATTGTATGACAAACAAAAGCAAAACAAAATTAATACTGAACTCTGTCTCATTCTAGCAATCAATAAATA
AAATAAATCCTGTTATTTTATGGGTTCATTAATTAATTGGACCACAAAATTCATTTGTGGGGGGTCCATT
TCATTAAGAGCTGAATTTTCCCCCAAAATTATGTTTTAAAAAGCTTTTTTTGAGATAATTGTAGATTGAC
ATGCACATACAAGAAAGGGTACAGAGAATTCCATACCCTTCACCCAGTTTCCCCTAACAAAGGTTACTTT
TATATTTATTTATTTATTTTTAGAGACAGAGCATTCTTCTGTTGCCCAGGCTGGAGTGCAGTGGTGTGAT
CTGGGCCCACTGCAACCTCCGCCTCCTGGATTCAAGTGAGTCTCTTGCTTCAGCCTCCCTAGTAGCTGGG
ATTACAGGCACCCGCCATCATGTCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGATTTCACCATGTTA
GCCAGGCTGGTCTCAAACTCCTGAACTCTAGTAATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTAC
AGGTGTGAGCCACCATGCCTGGCCAAAGGTTACTTTTAATGTTCATTAATTTTGAAGACTTGCAGTTTAT
TTATGCTATAAAGGACAATTGTGCACTATTAATAGTCGCAATGATGACATAATCTAAGACTTTTAATATT
TAGAAACTTATGGTTGCAGGAAATTAAAAATTCATCTCATGTGCTTTTTTGGCAGAGAAGTATGAAATAT
TGATCAATATAAGGTCCTTAAGCATATGCACCTATTAAACTTTTATGGAGGAAGTAGAATAGAATTCATA
AGTCCATTTGTTTTTACTCTTTATTTTTTTGAGACAGAGTCTCACTCTGCCGCCCAGGCTGGACCGCAGT
GGCACAATCTCCACTCACTGCAACCTCTGCCTCCTTGGTTCAAGTGATTCTCGTGTCTCAGCCACCTGAG
TAGCTGGGATTGCTACTCAGGTGTGCACCACCACACCCGACTAATTTTTGTATTTTTAGTAGAGATGGGA
TTTCACCCCTTTGGCCAGGTTGGTCTTGAACTCTTGGCCTCAAGTGATCTGCCTGCCTTGGCTTCCCAAA
ATGCTGGGATTACAGGTGTGAACCATCGCGCCTGGCCTAGAATTCATAAGTTTAAGGAGAAAAAGGAGTG
ATGAAAAATTTCCAAAAGTTAAGAACTTTTTCTTGTATTTTAAAAATGAGTGACGGTTGAAACAAAATTG
ATATATTATTTATGTGCTATTAGAAATAATTAAAAAGAATAAGGTTTTCTTTTTTAAAAATCAACATTTA
CTTTGGAAATTATGTCCTTTATAGTGATGAAAAATGTTTGATGTCAAGTTAAAAGTGAGTAAAGAAGTAC
ATTCATTTTATAACAGCATTTTTCACAGTAGCTAAAATGTGGTAGCAACCCAAGTGTTTATTACTGGATG
AATAGATAAACAAAATGTGGACTCAGGCATCTCTCTTCTGGGCTCATACCCAAAGGAAATGAAATCACCA
CCTCACCAAGGTATCTGTACTCCTGTGTTTATTGTAGCATTATTCATAATAACCAAGATATGGAGACAAC
CTAGACAACCATCAATGGACGAATGGTTTAAGAAATTGTGGTACGGATATATACAAGGGAATTCAGCCTT
AAAAAAGGAGGAGATAGCATTTGCCATAACCTAGACGGGCCTGGAGGACATTATGCTAAATGAAAGAAGC
CAGACACAGAAAAAAAATATTGCACGATCTTACCTGTATGTGGAATCTTTTATTTATTTATTTTCTTAGA
CGGAGTCTCACTCTGTTGCCCACGCTGGAGTGCAGTGGCGCAATTTTGGCTCACCGCAACCTCTGCCTCC
TGGGTTCAAGTAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCACCTGCCACCACACCTG
GCTAATTGTATTTTTAGTAGATATGGGGTTTTGCCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCA
GATGATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCTACCATGCCTGGCCTGTGG
AATCTTTTAAAAAGGTCAAATATATAGAGAATAAAACAGTGGTTATCAAGATTGGAGTAGGAGAGAGGAA
ATGGGCAGATGTAGGCCTAAGGATACGAAGTAGCAAACATATAGGATGAACAAGTCAAATAAAGTACAAT
ATGAAGACTACAATTAATAATAGCATATTATTCCAGGATTTTTGCTAGATGAGCATAGTTGCTTTTGCTA
CAGGAGGAAATAAATGGGTAACTAAGTGAGATGATGCATAAGTCAATTTGTTTCACTATAGTATCCATTC
TACTAAATATGTGTGTCTTATACCATGATGTTGTATATACCTTATATATACACAATAAAATTTATTTAAA
CAAACAAAATGTGGTATATACATAGAGTGGAATATTATTCAGCCTTAAAAAGGAAGTTCTGACACATGCC
ACAATATGGATGAATCTTGAGGATATTATGCTAAGTGAATTAAGCCAGTCACAAAAAACCAAATACTGTA
TGATTCTATGTATTTGAGGTATTTAAAGTAGTCAAAATAATAGAGACAGAAAGTAGAATGGTGGTTGGGA
CAGGCTGGGGAGAGGGAGGAGTGGGAGTTATGTTTAACGGTTATAGAGTTTTAGTTTTTCTAGATGAAAA
GAGTTAGAGAGAAGGATGGTGGTGAAGGTTGCACAACAATGTGCATGAACTTAATACCACTGAACTGTAC
ACTTAAATGTTAAAATGGTAAGATTGTTATGTGTATTTTGCCACAATAAATTAGTATTATTATTTTTTTT
AGAGACAGAGTCTAGCTCTGTCAACCAGGTTGAGTGCATTGGTGTGATAACAGCTCACTGCAGCCTCCAA
CTCTTGAGCCCCCAACTCCTGGCCTCAAGCAGTCCTCCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAG
GGGTGAGCCATTCACTGTTCCTTGCTAAATCTTTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTC
GCTCTGTCACCCAGGCTGGAGTGCAGTGGTGCGATCTCAGCTCGCTGCAAGCTCCACCTCCTGGGCTTAA
ATGATTTTCCTACTTCAGCTTCCTGAGTAGCTGGGATTACAGGCACACGCCACCATGCCTGGCTAATTTT
TGTATTTGTAGTAGAGACAGAGTTTTGCCATGTTGGCCAGACTGGTCTCGAACTCCTGACCTCAAGTGAT
CCACCTGCCTAAGCCTTCCTAAGTGTTGGGATTACAGGCATGAGCCACCACACCTGGCCTAAATTTTTTT
TTTTAATGAGTGAAGGAGTCCATAGTCTTTCATACTTTGGGGTGATACAAGAAAAGGAAAAAGGTTTAAT
GACAACTGCCACTTTCATTTATTCATTCCTTTGTTGATGTACTTTTGGGCTGGGTTTTGCTGTTATAAGC
TAAACTACAATGGACATCCTTGTACACATTCCCTAGAGTCCAAGTACAAGATTTTCTCTAGGGTGTTTTC
CTGGAAAAGGAATGACTTGAGGAGTATGGCAACCTTGTGAGAAATGACTGATTCGAAAGATGTGTCCCAT
AGCTTTTGATACATTTTTCTCCGAAGTCATGGTTCCAGTCCACACTCACACCAGCTGTGGAGGAGTTTTC
ATTTACCCACGAGCTTTGTTATTTCTGGTTTTGTCAGTCTTTTCAATTCTGCCAATCTCATAGGGTGTGA
TGGTATCTCGTTGTTTAAACCTGACTTCTCTTGTTAAGGTTGAGACCGAGCATCTGGCCCCATGTTTGCT
GGCTATTCAGCTTTCCTCTTCTGCAGACTGCCCGTTTGTCCACTGCTCTGTTTCAGTAACTTCCATATAC
CAAGAACTCTCTGCCTTCCATAACTCACATCACTTAAAATACGAATTTTTTTGATGCTTAGAGAACCTGA
TTTCCTTCCAACCAATTGTGCTCTCCTAATTCCAACAACCAAATGAAGCTTCAACTCTTCTTTTTGTTCA
GAGCCCTCCTGAGTCCTGTCCTACTCACAGTAAAGGCTGTGGCAAAATAAATAAATAAGTAAATAAAGGG
GAAAAATACACTGCATCCAAAAAAGGCTCGTTTCTTTTTCTTTCTTTCTCTCTCTCTCTTTCTTTTTCTT
TTTCTCTTGTTGTGACAATTGTCACAACAAAATGTGTATGTGACAATTTGTTATCTTAAAACAGTTTACC
CAAAATAGAAACCTTACAAAGCCAGTGGAAACTTTTCTTTTTGCATAAGTTGGTTAATTCTTTTGAGTGC
TGGCCTGGGATCCATGAAATAACCTCAACTCGACCATAAAACTTTTCACTCCCACGTTATGCAAACGGTG
ATGACCTTGTTGGTGGCAAGCCCCCCGTGTGACTTTCCGAGGGGATCTGAGTGGTGCAAGGTGGAGGACG
CAGTGATGGAAACATTGCGAGAGGGAAGGCGTCTGTTTCCACCCACTTACCCTCAGAGCATGAGGGCTGG
GCGAAGGCTCTGACTCCTGTAGGGGGTGACCCATTTCTAGGACTATAGAAGGAGAGGTGTGGACCTGGAA
AAAGGAAGGAAAGACCACGGCAAGGAAAGTCAAGAAGTGGGAGAAGTCAAGGGCTCTCTCCTCCCTTCCT
CCTTGTCCTTGGCCTGCAGCAACTCCTCTTTCTCTTTTTGTGGGGACAGACTGAAGAGGATTTCTTAGTA
TGGTTTGTACCTTCCCAGTAGAGCAGGAAAAAGAGGAACGAGCTGCTCCCTCCACACCTCAGACGCTGAG
GTCAACTGCCCTTTGGGCGGGGCACGTTGGCTCACGCATCTAATCCCAGCACTTTGGGAGGCCGTGAGGG
GAGCATCACTTGAGCCCAGGAGTTGGAGACCGGCCTGGGCCACATAGTGAGGCCCCATCCTCCGGCTTGC
ACCACTCAAATTCCCTCGTAAAGTCACACGGGGAGCTTGCCCCCAGCAAGGTCGTCAGTTTGCAAAACAT
GGGAGTGAAAAGCTTTATGGTGGAGTGTAGGTTATTTCATGGATCCCAGGCCAGCACCCAAAAGAATTAA
CCATCTTGTGCAAAAAGAAAATTTTCCACTACTTTTTTAAAGTTTCTATTTTGGGTAAATCGTTTCAAGA
TAACAAATTGGAGAGAGACCTAGAGAGAAATGAGCCTTTTTAAGATGCAGTTTATTTTGCCCCATGATGT
TTTTGTTTTTGTTTTTGCCAGAGCCTTTACTGTGAGTAGGACAGGACTCAGGAGGCCTCTGAACAAAAAG
AAGCGTTGAAGCTTCATTTGGTTGTTGGAATTGGGAGAGCACAATTGGTTGGAAGGAAATCAGGTTCTCT
CAAAAAATAAAATAAAATAAAATAAAAAAAAAAGCCAGCGTGCTGGCGCGGGTCCATGGTCCAAGCTACT
TGGGAGGCTGAGGTGGGGGGATCGCTTGAGCCCGGAAGTCGAGGCTGTAGTGAGCCGTGATTGCACCACT
GCACTCCAGCCTGGGCGATAGAACAAGACCCTGTCTCTTAAAACAAACAAGAAACAAAACAAAACAAACA
ACAAAAAAATTCATTTGGGAATGTTTCTGCGCGTGCCCATAAGCAGAGCCATGCTATAGGATCTCCCCTG
TGCCCCAACAATCAGCTTCTTACTTGGAGGGTAGAGAACGGTGCTTCCCCACGCTGCTGTGCAATGGATA
GGAGCTATGTGAAGCTGGCGTATGGGTGGGGTTTGCAGGCTTCGTCCGGCTTCATCGCCGGCTGCTGACC
CGGCACCAATTCCTGTTCTGCAGGTCTCACCGCAGAGGGGCACGCCAGCCATGAGGACAGATGAGGGAAC
ACGTGATGCCACGATGGGGGTGCCAGGATGAGGTGGGTGCGGTCGCGGACAGGCGCACGAGGAGCCCAGC
GGAGCGCCACCCGGAGCAGGCGCGGAGGAGGGCTGGGGAGGGCCACCAAGGCGACGAGAGCCGGTGTGCC
TGAATCAGCCTAAAGGAGACGGAGGAGGAGTGTGGTGGGCGCAGGGGCAGGGAGCTGGGGGAAGGGCGGG
GGGCTAGCCCAGGCTGAAGGCAGGCAGGAGCAGGGCCGCGATGTCAGACAAGAAACGGCAGCAGTGTGTT
TGGGAACCTAGCACCAACCGCACCGGTGGAGACAGGGTCTGCTGTAGAGGAGGTGGGGCCGGGTCCCAGC
TAAGTAAGGCGGTGGATCTTGCAGCCCTTCCATCCTCAGCCGCTCATTCTGCGCAAATCTCGGGGCCAGC
CTTGGTGGAGCCGTAAAGCGTCCACCAGAACCTGGATCCCTCCGCCACCTTTCCCATGAATTCACCTTTC
TGTACACAGCAAGCGCCTGAGCGGAGACGGCCGACACGTTTCCCACTGTTACCCCAGGAAACCGCGGCTC
CTGAGGGGGTCAGGGCCTGGGCAGGGGGCAGAGCTCAGCGCGCCGTACTGAGGCAGAAACGGGGTCCAGA
GAGGGTGGGGTGGGGGTACAGGGAAGGGTCGCCCGAAGGTCCCTGGCGCAGGGAGGACAGAGGAGGGATC
TAGAATTCGTAGGGGAGAAGAGAACTCAGAAAAGATCCGGCCCAGCGCATTTATTTTACAGA

Protein Sequence : Show Sequence

>gi|225007536|ref|NP_001139345.1| T-cell surface glycoprotein CD8 alpha chain isoform 1 precursor [Homo sapiens]
MALPVTALLLPLALLLHAARPSQFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFL
LYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGYYFCSALSNSIMYFSHFVPVFLPAKPTTT
PAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCNHRN
RRRVCKCPRPVVKSGDKPSLSARYV

Molecule Role : Vaximmutor
Related Vaccine(s): Recombinant Aeromonas hydrophila vaccine (Aera)

2. IFN1

Gene Name : IFN1
Sequence Strain (Species/Organism) : Ovis aries
NCBI Gene ID : 492823
Genbank Accession : AMGL02007291
Taxonomy ID : 9940
Chromosome No : 2
Gene Starting Position : 89212449
Gene Ending Position : 89212898
Gene Strand (Orientation) : +
Protein Name : transcript variant X1

DNA Sequence : Show Sequence

>NC_019459.2:89212449-89212898 Ovis aries breed Texel chromosome 2, Oar_v4.0, whole genome shotgun sequence
AATGGCCGCAGCCTGGTCCTTGCTCCTGGCCCTGCTGCTGCTCAGCTGCAACGCCATCTGCTCTTGGGGC
TGCCACCTGCCTCACACCCACAGCCTGGCCAACAGGAGGGTCCTGACACTCCTGCAACAACTGAGGAGGG
TCTCCTCTTCCTCCTGCCTGCAGGACAGAAAAGACTTCGCATTCCCCCAGAAGGCGCTGGGCGGCAGCCA
GTTGCAGAAGGCTCAAGCCATCTCTGTGCTCCACGAGGTGACCCAGCACACCTTCCAGCTCTTCAGCACA
GAGGGCTCGGCCGCCGCGTGGGACCAGAGACTCCTGGACAAGCTCCGCGCTGCACTGGATCAGCAGCTCA
CTGACCTGCAAGCCTGTCTCAGGCAGGAGGAGGGGCTGCGGGGGGGTCCCCCGCCCCAGGAGGGGGCGCG
GTTGCAGGAGAGATTCAGGAGCAAGGACTG

Molecule Role : Protective antigen
Related Vaccine(s): Recombinant Aeromonas hydrophila vaccine (Aera)

3. IgM

Gene Name : IgM
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16019
Genbank Accession : AC073553
Taxonomy ID : 10090
Chromosome No : 12
Gene Starting Position : 171229
Gene Ending Position : 175133
Gene Strand (Orientation) : -
Protein Name : immunoglobulin heavy constant mu
Protein Note : Also known as Igm; muH; Igh6; Igh-6; Igh-M; AI326478; MGC107036; MGC107507

DNA Sequence : Show Sequence

>gi|121699722:171229-175133 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
GTCATTTCACCTGCAGGTGACAGAGACAGTGTGAATTGCTGTCCACTTGTAGGGAGTAGAGGTAGAGGCA
GTGAGGACTGGTGACTCCCTGTCCCAGCCCTGTGTCCTCAGCCCCACAACCACACTACCTTGAACAGGGT
GACGGTGGTGCTGTAGAAGAGGCTCAGGAGGAAGAGGACGATGAAGGTGGAGGCAGTGGTCCACAGGTTC
TCAAAGCCTTCCTCCTCAGCATTCACCTCCCCCTCTATGAAGGGACACAGAGAGGGAGGGTCAAGCCAAG
TCTTGGGGTGACTGAGCATTTGCATAAGGGTTGGGGTGCCCTGATGGTTCTGGAGAATCTTAGCGAGTAT
TGACTGGTCCCCTAATAGCTCAAGCTAGACGGAGGCACAGAACAGGCCAAGCTGTAGGACACACTAATTT
GGAGCCTTTGTTCCAGGAGCCCTGGGGCAGCAGAGCAAGCAAGACAGATGCAATGTATGGACCTGGGTAA
AGGCAGACTCAGGAACTGTGGCAAAATGATATTGGTGTGCTCTGGACTTTCCCTGAGCAGGTGGTGCCAT
CGGTGCTGTTTAGACTGAGAATTTAGAGCAGGTCTCCCTGTCTCTTGGGCTTTAGCAATAAGAACAGGGA
TACCAGAGGTTGTCCCTCCTTACTTCCTGAAGGACTCAGGCATCTCATGACTTTCTTTGATCAGAGTTCC
CGTGCGTTGCTTGTCCTACCCCTGCCAGCAATGTTGGGTCTTGGTACCAAGAGATAAACAGACAGATACC
TGAGGGAGGCTGTGGGACCAGGGCATTTATTGGGATCTCTGATCCCTGTAAAGATGAGCCCTTGGGGATA
TCTAAAGCCTAGGCCCCAAATGGATATGAATATGAAGAATGTCCAGGATATGAGCCCAAAGAGGCAGTGT
TATTGGGTGGTGACTCCTTACAGAGGCTCCTTGATGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
GTGTGTGTGTGTGTGTGTGTGTGTGTGGTTGGGGCATCTTTTGGACAACTGCATTATGTTTTCTGCATTT
TGAACTGGAGATCAGGGTGGGTGTGAGTGGGTTAGGCAATTTCACATTGGTCTTCTGAGGTGTTCTATTA
ATGGAGACACAGCCAAGGCTAACTTTCTGTCATGGGTGGTTAGTGGAGTATCTTCTGCCCCATGGTGTTG
AGACAGATAGGCATACTTTGTACCTGGGGTTATCTCTCCCAATCATAGAGTGGGCTCCTGAGGAGTATCA
GTGACCCAGCCTAGTGTAGGCTCTCTGAGCCGTGAGCCTTGGAGATTACAGCCCTGGTCAGAGTCCAGGT
GTATCTGTGTGCTATACCTGTTCACATGTTAGTGTACACATGTGAAGATGTTTCTGTACATGTGTGTATA
TGCATATCTTTGTATCTATATAGTTTATTTGTGTATATTTGCCTATAGTCTGTTTCTGTGTACACAGGAC
CATGCATATTGGTATCTGTTTCTGTGAGTGGTTGTGTGTCTATGCCTATTTGTCTCTGAAGGCGTTTGTA
AGTGTCCATGCATGTGTGTGCGCATGAACTCATGTGTATATATGTGTACCTTTGTCTGTGTGTCCATGAA
TACACATTCATGTATGTGTCTACACATGTGTACCTATATATCTATGTATAAATGCATGTGTGTCTATATA
TGTGCCTGAATGCTGCCTGCACCAGGTTAGGCAAGCTTTCAGCCATATATCTAGAAGTAGCTGGAGGAAT
CGCATGTTCATACTCTGTCTGTTGTAAGCCTGACTGAGTTCACACACAAGGAGGAAACTGGCAAGGTAGA
GGAAGGGCCAGGGTGGAGGCAAGTATGCAGGGTGTGTGCAGAGCAAGCAAAACACAACTGTCTCAGCAGG
CATGAGCATTGTATAATCAAAACCAGTGACGTTTGAATGGATTTTTTTTATTTCTAATTTTTATAAAATG
CAACATCTCACTCTGACATGGTTAGTTTGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGA
GCGCTAGCATGGTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTACAGTGTGGGT
TTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGTGGCAGGGCCTCGTGGCCTACAACACAGG
TATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGCTGTGGGTAAAGTAGAAGCCTGG
GGCCCCAGGCTCTGGCATCGGGGCACTGGTCACATACTTCTCTTGGGGCAAGAGTTGCCCTCTCTGAAGC
CACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAGGCAGGTGACTGTGGCTGACTCCCTCAGGT
TCAGTTGCTCACGAGCTGGTGGCAGCAGGTACACAGCAGGTGGATGTTTGTGCACCTCTGTAAATGAAGA
CAGTAGTGAGGATAGGGTGGAAGAGGACCTGCCCTCCCTATGAGGTACAGGAAGGGTCCTGCAATTGGAG
GGGAAGGGAAGGGGGGATACCTACCATTGGGTTTTGAGATGAATTTCTTCTGTGGTGAAGGCAGATCCCT
GTGAGTCACAGTACACACAAATTCCTTCCTGTTATTCCAGTCTTCCACACAAACACTAGCCACACCCTTA
GCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTTGGTTTCCAGTGGTTCACCACTTTGAG
AAGCCCAGGAGATATTCAGGGTTTCATAGGTTGCCAGGTTTGAGACCAGACAGGTCAGGTTAGCGGACTT
GCTGAGGAAGATGTCGGCAAAGGAGGGGGGGATGGTGAAGGTTAGGATGTCTGTGGAGGGACCTGCAGTC
AAGAGAACACTGTGTCAGGTTATGACATATCTAGTTGTGAAGTTGGAAAAAAGCTTAGTCTCCATCCCCC
AAACCTACTTAAGGGAAGATGCTGGTCCTTCCAGCTGATTCTCACCCCTCTACTGCCTATTGCTCATTCT
TGTCCTTGGCCACTCTGGGGAGCCAAAGTTCAAGGAGCAAATGACCATGTCTGGGTGGCATTGGCCATAA
TTGTAGGAGGACTTCCCTAATCTGGGAGGGCTAGGCATTGGGCTTAGCCCAGGCCACTCACTGGCAGCAC
ATGTGGAGGACACGTTCTTCAAGAAGGTGAGACCCCTGTGATCCACACGGCAGGTGTACACATTCAGGTT
CAGCCAGTCGATTTCAGAGATGGTAAGTGTGCTTATGACCTTGTAGGTTTGGGGTGTGGATCCTTTGTTC
TCGATGGTCACCGGATCTGTGGTGAAGCCAGATTCCACGAGCTTCCCATCCTTTAGCCAGGATACTGTGA
TCGGTTTTGGAGTGAAGTTCGTGGCCTCGCAGATGAGTTTAGACTTGCGTGGTGCAGGGCCAGAGAAGCC
ATCCCGTGGTGGGACGAACACATTTACATTGGGGTTCATCTCTGCGACAGCTGGAATGAAAGGTCAAGGT
GTGAGCTCAGCTTGGCCTAAGCCCCACCCCGCTGCTCCCTCTGGGCCATGCCTGGGCCTGGGCACCCCTG
CTGGGAGGGTTTGGTTCTTACCTGGAATGGGCACATGCAGATCTTTGTTTTTGCCTCCGTAGTGGATTTT
GCATACCAGGTATTCATCTGAACCTTCAAGGATGCTCTTGGGAGACAGCAACACCTGCGAGGTGGCTAGG
TACTTGCCCCCTGTCCTCAGTGTTGGGAAGGTTCTGATACCCTGGATGACTTCAGTGTTGTTCTGGTAGT
TCCAGGTGAAGGAAATGGTGCTGGGCAGGAAGTCCCGGGCCAGGCAGCCCATGGCCACCAGATTCTTATC
AGACAGGGGGCTCTCGCAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTC

Molecule Role : Vaximmutor
Related Vaccine(s): Recombinant Aeromonas hydrophila vaccine (Aera)

4. IL8

Gene Name : IL8
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 3576
NCBI Protein GI : 10834978
Genbank Accession : ABBA01022993
Protein Accession : NP_000575
Taxonomy ID : 9606
Chromosome No : 4
Gene Starting Position : 74606222
Gene Ending Position : 74609432
Gene Strand (Orientation) : +
Protein Name : interleukin 8
Protein pI : 9.09
Protein Weight : 10403.36
Protein Length : 99
Protein Note : Also known as NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1

DNA Sequence : Show Sequence

>gi|224589816:74606222-74609432 Homo sapiens chromosome 4, GRCh37.p10 Primary Assembly
TGAGGGTGCATAAGTTCTCTAGTAGGGTGATGATATAAAAAGCCACCGGAGCACTCCATAAGGCACAAAC
TTTCAGAGACAGCAGAGCACACAAGCTTCTAGGACAAGAGCCAGGAAGAAACCACCGGAAGGAACCATCT
CACTGTGTGTAAACATGACTTCCAAGCTGGCCGTGGCTCTCTTGGCAGCCTTCCTGATTTCTGCAGCTCT
GTGTGAAGGTAAGCACATCTTTCTGACCTACAGCGTTTTCCTATGTCTAAATGTGATCCTTAGATAGCAA
AGCTATTCTTGATGCTTTGGTAACAAACATCCTTTTTATTCAGAAACAGAATATAATCTTAGCAGTCAAT
TAATGTTAAATTGAAGATTTAGAAAAAACTATATATAACACTTAGGAAAGTATAAAGTTTGATCAATATA
GATATTCTGCTTTTATAATTTATACCATGTAGCATGCATATATTTAACGTAAATAAGTAATTTATAGTAT
GTCCTATTGAGAACCACGGTTACCTATATTATGTATTAATATTGAGTTGAGCAAGGTAACTCAGACAATT
CCACTCCTTGTAGTATTTCATTGACAAGCCTCAGATTTGTCATTAATTCCTGTCTGGTTTAAAGATACCC
TGATTATAGACCAGGCATGTATAACTTATTTATATATTTCTGTTAATTCTTTCTGAAGGCAATTTCTATG
CTGGAGAGTCTTAGCTTGCCTACTATAAATAACACTGTGGTATCACAGAGGATTATGCAATATTGACCAG
ATAAAAATACCATGAAGATGTTGATATTGTACAAAAAGAACTCTAACTCTTTATATAGGAAGTCGTTCAA
TGTTGTCAGTTATGACTGTTTTTTAAAACAAAGAACTAACTGAGGTCAAGGGCTAGGAGAATATTCAGGA
ATGAGTTCACTAGAAACATGATGCCTTCCATAGTCTCCAAATAATCATATTGGAATTAGAAAGGAAGTAG
CTGGCAGAGCTGTGCCTGTTGATAAAATCAATCCTTAATCACTTTTTCCCCCAACAGGTGCAGTTTTGCC
AAGGAGTGCTAAAGAACTTAGATGTCAGTGCATAAAGACATACTCCAAACCTTTCCACCCCAAATTTATC
AAAGAACTGAGAGTGATTGAGAGTGGACCACACTGCGCCAACACAGAAATTATGTAAGTACTTTAAAAAA
GATTAGATATTTTGTTTTAGCAAACTTAAAATTAAGGAAGGTGGAAATATTTAGGAAAGTTCCAGGTGTT
AGGATTACAGTAGTAAATGAAACAAAACAAAATAAAAATATTTGTCTACATGACATTTAAATATGGTAGC
TTCCACAACTACTATAAATGTTATTTTGGACTTAGACTTTATGCCTGACTTAAGGAATCATGATTTGAAT
GCAAAAACTAAATATTAATCTGAACCATTTCTTTCTTATTTCAGTGTAAAGCTTTCTGATGGAAGAGAGC
TCTGTCTGGACCCCAAGGAAAACTGGGTGCAGAGGGTTGTGGAGAAGTTTTTGAAGAGGTAAGTTATATA
TTTTTTAATTTAAATTTTTCATTTATCCTGAGACATATAATCCAAAGTCAGCCTATAAATTTCTTTCTGT
TGCTAAAAATCGTCATTAGGTATCTGCCTTTTTGGTTAAAAAAAAAAGGAATAGCATCAATAGTGAGTTT
GTTGTACTCATGACCAGAAAGACCATACATAGTTTGCCCAGGAAATTCTGGGTTTAAGCTTGTGTCCTAT
ACTCTTAGTAAAGTTCTTTGTCACTCCCAGTAGTGTCCTATTTTAGATGATAATTTCTTTGATCTCCCTA
TTTATAGTTGAGAATATAGAGCATTTCTAACACATGAATGTCAAAGACTATATTGACTTTTCAAGAACCC
TACTTTCCTTCTTATTAAACATAGCTCATCTTTATATTTTTAATTTTATTTTAGGGCTGAGAATTCATAA
AAAAATTCATTCTCTGTGGTATCCAAGAATCAGTGAAGATGCCAGTGAAACTTCAAGCAAATCTACTTCA
ACACTTCATGTATTGTGTGGGTCTGTTGTAGGGTTGCCAGATGCAATACAAGATTCCTGGTTAAATTTGA
ATTTCAGTAAACAATGAATAGTTTTTCATTGTACCATGAAATATCCAGAACATACTTATATGTAAAGTAT
TATTTATTTGAATCTACAAAAAACAACAAATAATTTTTAAATATAAGGATTTTCCTAGATATTGCACGGG
AGAATATACAAATAGCAAAATTGAGGCCAAGGGCCAAGAGAATATCCGAACTTTAATTTCAGGAATTGAA
TGGGTTTGCTAGAATGTGATATTTGAAGCATCACATAAAAATGATGGGACAATAAATTTTGCCATAAAGT
CAAATTTAGCTGGAAATCCTGGATTTTTTTCTGTTAAATCTGGCAACCCTAGTCTGCTAGCCAGGATCCA
CAAGTCCTTGTTCCACTGTGCCTTGGTTTCTCCTTTATTTCTAAGTGGAAAAAGTATTAGCCACCATCTT
ACCTCACAGTGATGTTGTGAGGACATGTGGAAGCACTTTAAGTTTTTTCATCATAACATAAATTATTTTC
AAGTGTAACTTATTAACCTATTTATTATTTATGTATTTATTTAAGCATCAAATATTTGTGCAAGAATTTG
GAAAAATAGAAGATGAATCATTGATTGAATAGTTATAAAGATGTTATAGTAAATTTATTTTATTTTAGAT
ATTAAATGATGTTTTATTAGATAAATTTCAATCAGGGTTTTTAGATTAAACAAACAAACAATTGGGTACC
CAGTTAAATTTTCATTTCAGATAAACAACAAATAATTTTTTAGTATAAGTACATTATTGTTTATCTGAAA
TTTTAATTGAACTAACAATCCTAGTTTGATACTCCCAGTCTTGTCATTGCCAGCTGTGTTGGTAGTGCTG
TGTTGAATTACGGAATAATGAGTTAGAACTATTAAAACAGCCAAAACTCCACAGTCAATATTAGTAATTT
CTTGCTGGTTGAAACTTGTTTATTATGTACAAATAGATTCTTATAATATTATTTAAATGACTGCATTTTT
AAATACAAGGCTTTATATTTTTAACTTTAAGATGTTTTTATGTGCTCTCCAAATTTTTTTTACTGTTTCT
GATTGTATGGAAATATAAAAGTAAATATGAAACATTTAAAATATAATTTGTTGTCAAAGTA

Protein Sequence : Show Sequence

>gi|10834978|ref|NP_000575.1| interleukin-8 precursor [Homo sapiens]
MTSKLAVALLAAFLISAALCEGAVLPRSAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKL
SDGRELCLDPKENWVQRVVEKFLKRAENS

Molecule Role : Vaximmutor
Related Vaccine(s): Recombinant Aeromonas hydrophila vaccine (Aera)

5. TNF-alpha

Gene Name : TNF-alpha
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 21926
NCBI Protein GI : 7305585
Genbank Accession : AB039224
Protein Accession : NP_038721
Taxonomy ID : 10090
Chromosome No : 17
Gene Starting Position : 35199389
Gene Ending Position : 35201995
Gene Strand (Orientation) : -
Protein Name : tumor necrosis factor
Protein pI : 4.75
Protein Weight : 24010.47
Protein Length : 235
Protein Note : Also known as DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434

DNA Sequence : Show Sequence

>gi|372099093:35199389-35201995 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
CTTTATTTCTCTCAATGACCCGTAGGGCGATTACAGTCACGGCTCCCGTGGGGAGCAGAGGTTCAGTGAT
GTAGCGACAGCCTGGTCACCAAATCAGCGTTATTAAGACAATTGGGTTAGATAAATATTTTGTTTTAAAC
ATAAGCAAAAGAGGAGGCAACAAGGTAGAGAGGCCAGGTGGGGACAGCTCAGCTCCGTTTTCACAGAAAA
CATGTCTGTCTGAAGACAGCTTCCCACACTGGGTCCTCCAGGACACCCCGGCCTTCCAAATAAATACATT
CATAAGCAAATAAATAAATAATAAATAAATAATAAATAATAAGTGCAAATATAAATAGAGGGGGGCTGGC
TCTGTGAGGAAGGCTGTGCATTGCACCTCAGGGAAGAATCTGGAAAGGTCTGAAGGTAGGAAGGCCTGAG
ATCTTATCCAGCCTCATTCTGAGACAGAGGCAACCTGACCACTCTCCCTTTGCAGAACTCAGGAATGGAC
ATTCGAGGCTCCAGTGAATTCGGAAAGCCCATTTGAGTCCTTGATGGTGGTGCATGAGAGGCCCACAGTC
CAGGTCACTGTCCCAGCATCTTGTGTTTCTGAGTAGTTGTTGAAAGCTCTGAGCACAGAGTTGGACCCTG
AGCCATAATCCCCTTTCTAAGTTAGAAGGATACAGACTGGGGGCTCTGAGGAGTAGACAATAAAGGGGTC
AGAGTAAAGGGGTCAGAGTGGGGGCTGGGTAGAGAATGGATGAACACCCATTCCCTTCACAGAGCAATGA
CTCCAAAGTAGACCTGCCCGGACTCCGCAAAGTCTAAGTACTTGGGCAGATTGACCTCAGCGCTGAGTTG
GTCCCCCTTCTCCAGCTGGAAGACTCCTCCCAGGTATATGGGCTCATACCAGGGTTTGAGCTCAGCCCCC
TCAGGGGTGTCCTTGGGGCAGGGGCTCTTGACGGCAGAGAGGAGGTTGACTTTCTCCTGGTATGAGATAG
CAAATCGGCTGACGGTGTGGGTGAGGAGCACGTAGTCGGGGCAGCCTTGTCCCTTGAAGAGAACCTGGGA
GTAGACAAGGTACAACCCATCGGCTGGCACCACTAGTTGGTTGTCTTTGAGATCCATGCCGTTGGCCAGG
AGGGCGTTGGCGCGCTGGCTCAGCCACTCCAGCTGCTCCTCCACTTGGTGGTTTGCTGAGGGGGGGGGGG
AGGATTGAGTCAGTGTCACCCTCTTAGTTCACACTCCACATCCTGAGCCTCAGCAGCTACCCACACTTCA
CTTCCGGTTCCTGCACCCTCTGTCTTTCCACATCCCATTGGCTATGAGGTCCCGGGTGGCCCCCTGATGC
CTTGCTTTTGAGTCACTGCTCTGACTCTCACGTGCTGTCTCTAAGAGCTCTGTCTTTTCTCAGCCTGGCT
CGACACCCCTCAACCCGCCCCCCAAAATCATGCCCCTTCATTCTCAAGGCACATGTAAAGAAATCTTACC
TACGACGTGGGCTACAGGCTTGTCACTCGAATTTTGAGAAGATGATCCTGGAGGGGAAGAGACAAAGGCA
AGGATGAGCCTTTTAGGCTTCCCAGCAAGCATCTATGCACTTAGACCCCTTTCCTCCCAAACCAAAGCTT
TAAGTTCTCCCCCCACCCCATCTCATCCCATGCCTAACTGCCCTTCCTCCATCTTAAATTAAGAGAGAGG
TGTGGGAACACTTACTGAGTGTGAGGGTCTGGGCCATAGAACTGATGAGAGGGAGGCCATTTGGGAACTT
CTGTGTAGGAAAAGGAGGTTAGTTAAGACAGACTCACCCCAAAGGAGAAGCCTCCCGGCTGATTGCCCCG
CTTACAGTTCCTCTTTGCCCCACCCCACCCCCCAGCTTTGTGTTTTTCTTCTTCATTCATTCATCTGTCC
AACCCACGGCTTCTTTCTGCGGTGCCCTCTGTGCTTGATCTCCCGTTATCTCCCCTTCATCTTCCTCCTT
ATCTCTCATGCCTCTCTCATTTCTGTCTCTGAGTTTTATCTCTTGCTTATCCCCTCTTCCCCTGGCCACA
TCTTTCCAGATCTCTCCACGTGTGAACACACTTGTTCGTTCATTCATCTCTCTGTGCATCCGACGAAGGA
TGTTTAGTCAGCTGGACGCATGGGTCCGAGGTCCTGACTCTGTCCCCTCCACACTCTCCTCCACCTTGCC
CTGCCCATTAGCCCACTTCTTTCCCTCACACTGTCCTTCTTGCCCTCCTAACCCGTTTTGCTTGTGAGCG
AGAATAAGGGTTGCCCAGACACTCACCTCATCCCTTTGGGGACCGATCACCCCGAAGTTCAGTAGACAGA
AGAGCGTGGTGGCCCCTGCCACAAGCAGGAATGAGAAGAGGCTGAGACATAGGCACCGCCTGGAGTTCTG
GAAGCCCCCCATCTTTTGGGGGAGTGCCTCTTCTGCCAGTTCCACGTCGCGGATCATGCTTTCTGTGCTC
ATGGTGTCTTTTCTGGAGGGAGATGTGGCGCCTTGGGCCAGTGAGTGAAAGGGACAGAACCTGCCTGGTT
GGCTGCTTGCTTTTCTGGGAGCTATTTCCAAGATGTTCTGGAGTTTCTGTTCTCCCTCCTGGCTAGTCCC
TTGCTGTCCTCGCTGAG

Protein Sequence : Show Sequence

>gi|7305585|ref|NP_038721.1| tumor necrosis factor [Mus musculus]
MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPL
ISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQ
VLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQL
SAEVNLPKYLDFAESGQVYFGVIAL

Molecule Role : Vaximmutor
Related Vaccine(s): Recombinant Aeromonas hydrophila vaccine (Aera)

IV. Vaccine Information

1. Aeromonas hydrophila aroA mutant vaccine

a. Vaccine Ontology ID:

VO_0002799

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Gene Engineering of aroA

Type: Gene mutation
Description:
Detailed Gene Information: Click here.

e. Immunization Route

Intraperitoneal injection (i.p.)

2. Aeromonas hydrophila LSA34 vector vaccine WED/pUTDgap

a. Vaccine Ontology ID:

VO_0004606

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Antigen

The protective antigen GAPDH from the fish pathogen A. hydrophila, encoded by the gene gapA34 (Mu et al., 2011).

e. Vector:

The Edwardsiella tarda strain EIB202 is used a a live, attenuated vaccine vector, using the promoter P(dps) as the protein-based expression system for the antigen (Mu et al., 2011).

f. Preparation

The gapA34 gene was introduced into P(dps) and transformed into live, attenuated E. tarda strain EIB202 (Mu et al., 2011).

g. Immunization Route

Intramuscular injection (i.m.)

h. Description

A. hydrophila is a common fish pathogen, so in order to create a vaccine against this pathogen, the gapA34 gene of A. hydrophila was expressed in the bacterial vector E. tarda strain EIB202, and it was shown to increase survival of vaccinated fish (Mu et al., 2011).

i. Fish Response

Host Strain: Turbot (Scophtalmus maximus)
Vaccine Immune Response Type: VO_0000287
Efficacy: Over 60% of the fish vaccinated with the WED/pUTDgap vector vaccine survived the A. hydrophila challenge (Mu et al., 2011).
Description: The P(dps) promoter--which was chosen due to its high transcription activity-- was shown to elicit survival in turbot, meaning that it has the potential to be an important part of bacterial vaccine vector application (Mu et al., 2011) .

3. Recombinant Aeromonas hydrophila vaccine (Aera)

a. Vaccine Ontology ID:

VO_0004811

b. Type:

Recombinant vector vaccine

c. Status:

Licensed

d. Host Species for Licensed Use:

Baboon

e. Immunization Route

Intramuscular injection (i.m.)

f. Fish Response

Vaccination Protocol: In vaccination experiments, healthy grass carp were distributed randomly into control group and bath immunization group (150 fish per group). For bath immunization, fish were immersed in 20 mg L−1 SWCNTs-Aera and the control group was immersed in PBS for 6 h. At the end of the vaccination, grass carp were transferred to different tanks without SWCNTs-Aera for 28 d. After 28 d, 15 fish in each replicate were euthanized in the laboratory through washrag soaked with 20.0 g/m3 MS-222. Sampled fish were dissected immediately with sterile scissors. The intestine, kidney and spleen were aseptically removed from their abdominal cavity, separately, where five fish were sampled for analyzing expression of seven immune-related genes through quantitative real-time PCR (qRT-PCR) and the experiment was performed in triplicate replicates. Thereafter, the intestine of three fish (15 fish per replicate) were also collected and pooled together as described elsewhere to extract microbial DNA for deep sequencing [29]. After 2 d, the remaining fish (15 grass carp in each replicate) were intramuscularly injected with 6.5 × 104 cfu mL−1A. hydrophila, kept for 10 days and thereafter euthanized for sampling. Subsequently, the intestine was carefully excised and maintained as above, which was also for extracting microbial DNA for deep sequencing. All the samples were placed into sterile polypropylene centrifuge tubes and stored in −80 °C until DNA extraction. All experiments were performed in triplicate (Liu et al., 2015).
Host Gene Response of CD8A
- Gene Response: The expression of CD8A was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
- Detailed Gene Information: Click here.
Host Gene Response of IFN1
- Gene Response: The expression of IFN1 was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
- Detailed Gene Information: Click here.
Host Gene Response of IgM
- Gene Response: The expression of IgM was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
- Detailed Gene Information: Click here.
Host Gene Response of IL8
- Gene Response: The expression of IL8 was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
- Detailed Gene Information: Click here.
Host Gene Response of TNF-alpha
- Gene Response: The expression of TNF-A was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
- Detailed Gene Information: Click here.

V. References

1. Hernanz et al., 1998: Hernanz Moral C, Flaño del Castillo E, López Fierro P, Villena Cortés A, Anguita Castillo J, Cascón Soriano A, Sánchez Salazar M, Razquín Peralta B, Naharro Carrasco G. Molecular characterization of the Aeromonas hydrophila aroA gene and potential use of an auxotrophic aroA mutant as a live attenuated vaccine. Infection and immunity. 1998; 66(5); 1813-1821. [PubMed: 9573055].

2. Liu et al., 2015: Liu L, Gong YX, Zhu B, Liu GL, Wang GX, Ling F. Effect of a new recombinant Aeromonas hydrophila vaccine on the grass carp intestinal microbiota and correlations with immunological responses. Fish & shellfish immunology. 2015; ; . [PubMed: 25862971].

3. Mu et al., 2011: Mu W, Guan L, Yan Y, Liu Q, Zhang Y. A novel in vivo inducible expression system in Edwardsiella tarda for potential application in bacterial polyvalence vaccine. Fish & shellfish immunology. 2011; 31(6); 1097-1105. [PubMed: 21964456].

4. Wiki: Aeromonas Hydrophila: Aeromonas Hydrophila [http://microbewiki.kenyon.edu/index.php/Aeromonas_Hydrophila]