VIOLIN: Vaccine Investigation and Online Information Network

Edwardsiella tarda

Table of Contents

General Information
1. NCBI Taxonomy ID
2. Introduction
Vaccine Related Pathogen Genes
1. degP (Protective antigen)
2. dnaK (Protective antigen)
3. esa1 (Protective antigen)
4. eseC (Protective antigen)
5. eseD (Protective antigen)
6. et18 (Protective antigen)
7. eta21 (Protective antigen)
8. eta6 (Protective antigen)
9. eta81 (Protective antigen)
10. fimA (Protective antigen)
11. flagellar hook assembly protein FlgD (Protective antigen)
12. flagellin FliC (Protective antigen)
13. fliC (Protective antigen)
14. GAPDH (Protective antigen)
15. groEL (Protective antigen)
16. invasin (Protective antigen)
17. nanA (Protective antigen)
18. ompA (Protective antigen)
19. ompC (Protective antigen)
20. ompX (Protective antigen)
21. putative exoprotein-precursor (Protective antigen)
22. putative flagellin associated protein (Protective antigen)
23. aroC (Virmugen)
24. esrB (Virmugen)
25. esrC (Virmugen)
26. slyA (Virmugen)
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

2. Introduction

E. tarda is typically found in the normal gut flora of fish and humans, and can be an opportunistic pathogen in human, causing gastroenteritis and diarrhea. E. tarda has a high affinity for red blood cells due to specific fimbriae that it produces (Microbe Wiki: E. tarda).

II. Vaccine Related Pathogen Genes

1. aroC

Gene Name : aroC
Sequence Strain (Species/Organism) : Edwardsiella tarda EIB202
NCBI Gene ID : 8608790
NCBI Protein GI : 269139790
Locus Tag : ETAE_2445
Genbank Accession : CP001135
Protein Accession : YP_003296491
Taxonomy ID : 498217
Gene Starting Position : 2576311
Gene Ending Position : 2577396
Gene Strand (Orientation) : -
Protein Name : chorismate synthase
Protein pI : 6.99
Protein Weight : 36170.26
Protein Length : 361

DNA Sequence : Show Sequence

>gi|269137358:2576311-2577396 Edwardsiella tarda EIB202 chromosome, complete genome
TTTACCAGCGCGGTATCGTACAGGCGACATCCGCACACTGCGCACGCTGGCGCAGCAAATGATCCATCAG
CACCAGCGCCATCATCGCCTCGGCGATCGGCACCGCCCGGATCCCCACGCAGGGATCGTGGCGGCCGCGG
GTGACCATGGTCGTCGCCTCGCCGTCGCGGGTAATGGTCTGCCCCGGCACCATAATGCTCGACGTCGGCT
TCAGCGCCAGACGCGCCAGCAGCGGCTGACCGCTGCTGATCCCGCCCAGAATGCCGCCGGCATGGTTGCT
GAGAAAGCCCGTCGGGCTGATCTCGTCACGGTGCTCGCTGCCGCGCTGCCCCACCACCGCAAAGCCGTCG
CCGATCTCGACGCCCTTCACCGCATTGATGCTCATCAGCGCATGGGCCACGTCGGCATCCAGGCGATCGA
AGACCGGCTCGCCCAGCCCGGGGGGAACCCCGGTCGCCATCACCATCACCTCGGCGCCGATCGAGTCGCC
GCTCTTTTTCAGATCGCGCATCAGGGCATCCAGCGCCGCCAGCTGCTGGACATCGGGACAGAAGAAGGGA
TTCTGCTCTACCTGCGCCCAGTCATACTGACGGCAGACGATATCGCCCATCCGGCTCAGGCAGGCGCGGA
TAACGATGCCATGCTGCTGCTGAAGGTATTTTTTCGCGATCGCCCCGGCGGCGACGCGCATCGCGGTCTC
CCGCGCAGAGGAGCGCCCGCCGCCGCGGTAGTCGCGCAGACCATACTTCTGCTCATAGCTGTAGTCAGCG
TGACCCGGACGAAACAGATCCTTGATGGCGCTATAGTCCTGCGAGCGCTGATCGGTGTTCTCGATCAGCA
GTCCAATACTGGTGCCGGTGGTCACCCCCTCAAAGACACCCGACAGGATCCGCACCCGATCATCCTCTCG
CCGCGCCGTGGTGTAGCGCGATGCGCCGGGGCGACGGCGATCGAGATCGTGCTGCAGATCCGCCTCGCTC
AGCGCCATTCCCGGCGGAACGCCGTCCACAATGCAGCCCAGCGCCGCACCGTGTGACTCGCCGAAGGTGG
TGACTCTGAATACCTGACCAATGCTGTTTCCCGCCA

Protein Sequence : Show Sequence

>gi|269139790|ref|YP_003296491.1| chorismate synthase [Edwardsiella tarda EIB202]
MAGNSIGQVFRVTTFGESHGAALGCIVDGVPPGMALSEADLQHDLDRRRPGASRYTTARREDDRVRILSG
VFEGVTTGTSIGLLIENTDQRSQDYSAIKDLFRPGHADYSYEQKYGLRDYRGGGRSSARETAMRVAAGAI
AKKYLQQQHGIVIRACLSRMGDIVCRQYDWAQVEQNPFFCPDVQQLAALDALMRDLKKSGDSIGAEVMVM
ATGVPPGLGEPVFDRLDADVAHALMSINAVKGVEIGDGFAVVGQRGSEHRDEISPTGFLSNHAGGILGGI
SSGQPLLARLALKPTSSIMVPGQTITRDGEATTMVTRGRHDPCVGIRAVPIAEAMMALVLMDHLLRQRAQ
CADVACTIPRW

Molecule Role : Virmugen
Molecule Role Annotation : An aroC mutant is attenuated in zebra fish. The aroC mutant is also protective against challenge with wild type E. tarda and has a relative percent survival of 68.3% (Xiao et al., 2011).
Related Vaccine(s): Edwardsiella tarda aroC mutant vaccine , Edwardsiella tarda aroC/esrC mutant vaccine

2. degP

Gene Name : degP
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : ADB84596
Other Database IDs : CDD:236802
CDD:290102
CDD:238487
Taxonomy ID : 636
Protein Name : DegP
Protein pI : 7.41
Protein Weight : 45471.82
Protein Length : 517
Protein Note : serine endoprotease; Provisional

Protein Sequence : Show Sequence

>ADB84596.1 DegP [Edwardsiella tarda]
MKKTTLVLSAVALSLGMALSPMSASAAETSSATTAQALPSLAPMLEKVMPSVVSINVEGSATVNNSRVPP
QFQQFFGEDSPLCQPGSPFGGSPMCQGGGNAPSKERFKALGSGVIIDAGKGYVVTNNHVVANADSIKVQL
SDGRRFDAKVIGTDPRTDIALIQLKDAKNLTAIKMADSDNLRVGDYAVAIGNPYGLGETVTSGIVSALGR
SGLNVENYENFIQTDAAINRGNSGGALVNLNGDLIGINTAILAPDGGNIGIGFAIPSNMVKNLTGQMVEY
GQVKRGELGIMGTELNSELAKAMKIDAQRGAFISQVLPKSAAAKAGLKAGDVIVSLNGKNISSFASFRAE
IGTMPVGSKLALGIIRDGKPMNVEVTLEQSAQSQVASDNIFSGIEGAELSNAAGGKGVKVDNVKKGSTAA
RIGLQKGDVILGVNQHAIANIGELRKILDAKPPVLALNIQRGDNTLYLLMQ

Molecule Role : Protective antigen
Molecule Role Annotation : (Jiao et al., 2010)

3. dnaK

Gene Name : dnaK
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : AKH90009
Other Database IDs : CDD:234715
Taxonomy ID : 636
Protein Name : molecular chaperone DnaK
Protein pI : 4.57
Protein Weight : 64300.33
Protein Length : 704
Protein Note : molecular chaperone DnaK; Provisional; PRK00290

Protein Sequence : Show Sequence

>AKH90009.1 molecular chaperone DnaK [Edwardsiella tarda]
MGKIIGIDLGTTNSCVAIMDGGQPRVLENAEGDRTTPSIIAYTQDGEVLVGQPAKRQAVTNPQNTLFAIK
RLIGRRFEDAEAQRDKDIMPYKIVPADNGDAWVEVKGQKIAPPQISAEVLKKMKKTAEDFLGEPVTEAVI
TVPAYFNDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGLDKGAGNRTIAVYDLGGGTFDISIIEIDDV
DGEKTFEVLATNGDTHLGGEDFDSRLINYLVDEFKKEQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTD
VNLPYITADATGPKHMNIKVTRAKLESLVEDLVARSMEPLKVALKDAGLSVSDIDDVILVGGQTRMPMVQ
KQVADFFGKEPRKDVNPDEAVAIGAAVQGGVLAGDVKDVLLLDVTPLSLGIETMGGVMTALISKNTTIPT
KHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGIQPAPRGMPQIEVTFDIDADGILHVSAKD
KNSGREQNITIKASSGLSEDEIKQMVRDAEANAEADRKFEELVQVRNQADQLVHGTRKLVEEAGDKLPAS
DKSAIEDALKALEAASKGEDKAEIEAKIQALVQASAKLQELAQQQAQSGEAQADTSAKKDDDVVDAEFEE
VKDKK

Molecule Role : Protective antigen
Molecule Role Annotation : (Hu et al., 2012)

4. esa1

Gene Name : esa1
Sequence Strain (Species/Organism) : Edwardsiella tarda strain TX1
NCBI Protein GI : 302136428
Other Database IDs : CDD:236834
CDD:284624
CDD:279448
Taxonomy ID : 636
Gene Strand (Orientation) : ?
Protein Name : putative surface antigen
Protein pI : 5.14
Protein Weight : 82376.08
Protein Length : 866
Protein Note : outer membrane protein assembly factor YaeT; Provisional

Protein Sequence : Show Sequence

>ADK94171.1 putative surface antigen [Edwardsiella tarda]
MAMKKLLLASLLFGSATVYGADGFVVKNIHFEGLQRVAVGAALLNMPVRVGDTVSDEDLSNTIRALYATG
NFEDVRVLRDGNNLIVQVKERPTIASITFSGNKSVKDEMLKQNLEASGVRVGEALDRTTLSSIEKGLEDF
YYSVGKYNATVKAVVTPLPRNRVDLKLVFTEGKSAQIQQINIVGNHAFSSAELLGHFQLRDDVPWWNLMA
DRKYQKQKLAGDLEALRSYYLDRGYARFAINSTQVSLTPDKKGIYITINITESEQYKLEGVDVTGNMAGY
SAEITRLTKVEPGELYNGAKVTKMEDEIKQLLGRYGYAYPCVQTQPEINDKDKTVVLHMNIDAGNRYYVR
QIRFVGNDTSKDAVLRREMRQMEGSWLGSDQVEQGKERLNRTGYFENVDVETQRVPGTPDQVDVIYKVKE
RNTGSFNFGIGYGTESGVSFQVGVQQDNWLGTGNVVAINGTKNDYQTYAELSLTDPYFTVNGVSLGGRLF
YNDFKANDADLSDYTNSSYGFDGTLGFPINENNSLRTGLGYVHNDLSDMQPQVAMWRYLRSVGQNPSDSQ
RASYKADDYTWTAGWAYNNLDRGFFPTAGSKASLNGKITLPGSDNEYYKLTFDSQSYFPINQDRTWVVLG
RTRLGYGNGFGGKEMPFYENFYAGGSGTVRGFQSNNIGPKAVYLNGDGSVDQSKTGDNDAVGGNAMAVAS
LELITPTPFVSEQYANSLRTSVFMDAGTVWDTNWDQAAYPTLPDYSKATNVRLSAGIALQWMSPLGPLVF
SYAQPVKKYEGDKAEQFQFNIGKTW

Molecule Role : Protective antigen
Molecule Role Annotation : Together these results indicate that Esa1 is a protective immunogen and an effective oral vaccine when delivered by FP3/pJsa1 as a surface-anchored antigen.(Sun et al., 2010)
Related Vaccine(s): E. tarda DNA vaccine pCEsa1

5. eseC

Gene Name : eseC
Sequence Strain (Species/Organism) : Edwardsiella tarda EIB201
NCBI Protein GI : AAV69404
Other Database IDs : CDD:237952
Taxonomy ID : 636
Protein Name : EseC
Protein pI : 6.15
Protein Weight : 50243.03
Protein Length : 553
Protein Note : pathogenicity island 2 effector protein SseC; Provisional

Protein Sequence : Show Sequence

>AAV69404.1 EseC [Edwardsiella tarda]
MVRRQAAVPGDQGGNTASVHTGDRGDTAMNNITETRYTTPPLGGSGQNDSVQSQHDHPQIGAGALDPLRP
PPQRQQDHDPKREFHRPPAPRQPVTLRLAEGALNRILDIFGPRVDGKPLTVSDIERTPMEAMTLAASLLG
VKALGDNANLKALAMEIRTHGAENLRLRQNEDLRKQVEKSIEDQAKAQKAGIFSAIVDWIVSIAEVVSGI
GKILIGDFAGGAMDVAAGCAGLVKAACETLALIDKDNAEKYQSIADVAGKVQLAFEIAGMAVDLVSVGRG
VMASKSIAKATETVMEGAAGEALHTAVTQGSETAIKTAASEIGKQVAEQVADQVTRTLAEQSGRFLGQGH
LLEAFSKQAIEQMVTRAVEAAAKKALQSGVEMSAKELSKQVVKEVQREVIKAIVGACIDSVANNAKSTTR
STLQGVNSVYQGVITKERADLQKVIQQLINETDFMQFMLEEFEKIKKRAKEDIGSLMDGAGKALSAAADA
QQKSGAMLSNIAANIA

Molecule Role : Protective antigen
Molecule Role Annotation : (Song et al., 2013)

6. eseD

Gene Name : eseD
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : AAV69405
Other Database IDs : CDD:185259
Taxonomy ID : 636
Protein Name : EseD
Protein pI : 4.77
Protein Weight : 20636.43
Protein Length : 235
Protein Note : pathogenicity island 2 effector protein SseD; Provisional

Protein Sequence : Show Sequence

>AAV69405.1 EseD [Edwardsiella tarda]
MTTIDSGSHGVNGITPPDGHRYVSQDRGGDAIGQLNELMVQLGELFGKLRDVLRQYQQTQQSNAFKMQKT
AYDTRVDAIDKDFQAKQAQAIAQILGGIAQTFGGAFGEEAHTISNGVNSMTQGIVGVGYVNDMSRQSQEE
QALSEYQHGLAEQQLKRADETLEKALKISGDLREILTTLNQAHERIASSVRMS

Molecule Role : Protective antigen
Molecule Role Annotation : (Wang et al., 2010)

7. esrB

Gene Name : esrB
Sequence Strain (Species/Organism) : Edwardsiella tarda EIB202
NCBI Gene ID : 8607237
NCBI Protein GI : 269138242
Locus Tag : ETAE_0886
Genbank Accession : CP001135
Protein Accession : YP_003294942
Taxonomy ID : 498217
Gene Starting Position : 964657
Gene Ending Position : 965301
Gene Strand (Orientation) : -
Protein Name : two-component response regulator EsrB, LuxR family
Protein pI : 9.13
Protein Weight : 21902.65
Protein Length : 214

DNA Sequence : Show Sequence

>gi|269137358:964657-965301 Edwardsiella tarda EIB202 chromosome, complete genome
ATTAAAACTCCAGAACCCCCAGGCGGCGGGCCCAGTTGCTCAGCTCCGCCGCGTTGTGGGCATCCAGCTT
GCGCATCAGGTTGAGCCGGTGGGTCTCCACGGTTTTCAGGCTGATGGACAGCAGCTCCGCGATATCCCGG
TTGCGCTTGCCCTCGGCGACCAGCTTGAGAATTTGCCGTTCGCGCAGGGTCAGCGGCGGCTCCTGAGCCA
TATCGGACGAGGCTACCAGGGAGTCCAGCCGGAGGGCGGGATCGACAAAGGTTTTCCCCAGCGAGGCATG
CTGCATGGCCGCCAGCAGGGTTTGCTGCGGGCTTTGCTTCAGGACGTAGGCGAGCGCGCCGGCGGCGAGG
GCTTCGCTGCCGCGCCGTGCCTCAGAGGAGGCGGTGACGACGACGATACGCATATCGCTCCAGCGACGCC
GCAGCTGACGGATCACGTCAACGCCGTCCATGCCCGGCAGACCCAGATCGAGTAAGACCAGATCGGGCGA
GAGACGCTGGCAGGCCTCATAAACGGCCAGCCCATTCGAGACGGCGCCCACGACCCGGTAGCGGGGCTGG
CTGTCCAAGAGATTACGGATGCCATCGGCTAGCAAGGCATGATCTTCAACGATCAGAATGTTCAGAGGCA
AAATAGAAATAGTCA

Protein Sequence : Show Sequence

>gi|269138242|ref|YP_003294942.1| two-component response regulator EsrB, LuxR family [Edwardsiella tarda EIB202]
MTISILPLNILIVEDHALLADGIRNLLDSQPRYRVVGAVSNGLAVYEACQRLSPDLVLLDLGLPGMDGVD
VIRQLRRRWSDMRIVVVTASSEARRGSEALAAGALAYVLKQSPQQTLLAAMQHASLGKTFVDPALRLDSL
VASSDMAQEPPLTLRERQILKLVAEGKRNRDIAELLSISLKTVETHRLNLMRKLDAHNAAELSNWARRLG
VLEF

Molecule Role : Virmugen
Molecule Role Annotation : An esrB mutant is highly attenuated in fish (turbot), with an LD50 of 10(8.1)cfu/fish. Vaccination with this mutant elicited significant protection from wild type E. tarda (Lan et al., 2007).
Related Vaccine(s): Edwardsiella tarda esrB mutant vaccine

8. esrC

Gene Name : esrC
Sequence Strain (Species/Organism) : Edwardsiella tarda EIB202
NCBI Gene ID : 8607212
NCBI Protein GI : 269138217
Locus Tag : ETAE_0861
Genbank Accession : CP001135
Protein Accession : YP_003294917
Taxonomy ID : 498217
Gene Starting Position : 942933
Gene Ending Position : 943625
Gene Strand (Orientation) : -
Protein Name : putative transcriptional regulator EsrC
Protein pI : 9.57
Protein Weight : 25155.32
Protein Length : 230

DNA Sequence : Show Sequence

>gi|269137358:942933-943625 Edwardsiella tarda EIB202 chromosome, complete genome
TTTAGCCGGCGCGGTGGTGAAGGCTGGCCCGACGCACTTCGCTCGGTGACTGACTAAAGTGACGCCGAAA
GCAGTCAGAGAAGTGGGCGGCGTTACAGAATCCGGACTCCAGCGCGACGTCGATCACCTTTTTAGAGGTG
GTTTCGAGTAGCTGGCGGGCATGGCCCAGACGCTGGCGCAGTAGCCAGCGTTTCGGCGCCATGCCGAACT
TGTCGTTGAACAGCTGGTTGAACTTACGCTGCGACAGGCCGAAGAGATCGGCGTAGCGCGCGACCGGCCA
GGGTTCGAGCCGATGGCGGTAGAGCGTGTCGAACAGGTCGGCATCGGCGGTTAACAGCGATCGCAGCAGG
GTGGAGGTCCGTTGACACTCCAGGGTCAGGCTGTAGGCCAAGACCAGCCTGAGCATGGTTTCATCGTCGG
CATCCGCGAGCCGCAGCAGCGTTTTCAGCAGGCCCGGCGCCATGGCGATGGGGAGCGGCTGAGGGGGCGC
GACGGGGGCCGGGCCGCTGTACATCAGGTGCTGCAAATCGCGGCATATGCCGTGCAGCAGATTACACGGA
TAGCTAAATAGCCGAGTATGCCGGGTCGACGCGATGCCGGGAGGACGCGAGTCAGCGATCAGCAGCGCGC
CGGGCTCCGGCAGGAGCGCGATGTTGTCTGGACCGGTGACGAGCAGTAGATGCAAATTGGGCA

Protein Sequence : Show Sequence

>gi|269138217|ref|YP_003294917.1| putative transcriptional regulator EsrC [Edwardsiella tarda EIB202]
MPNLHLLLVTGPDNIALLPEPGALLIADSRPPGIASTRHTRLFSYPCNLLHGICRDLQHLMYSGPAPVAP
PQPLPIAMAPGLLKTLLRLADADDETMLRLVLAYSLTLECQRTSTLLRSLLTADADLFDTLYRHRLEPWP
VARYADLFGLSQRKFNQLFNDKFGMAPKRWLLRQRLGHARQLLETTSKKVIDVALESGFCNAAHFSDCFR
RHFSQSPSEVRRASLHHRAG

Molecule Role : Virmugen
Molecule Role Annotation : An aroC and esrC mutant is attenuated in zebra fish. This mutant is also protective against challenge with wild type E. tarda and has a relative percent survival of 71.3% (Xiao et al., 2011).
Related Vaccine(s): Edwardsiella tarda aroC/esrC mutant vaccine

9. et18

Gene Name : et18
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : 74474893
Other Database IDs : CDD:182907
Taxonomy ID : 636
Gene Strand (Orientation) : ?
Protein Name : antigenic protein Et 18
Protein pI : 9.61
Protein Weight : 18746.93
Protein Length : 257
Protein Note : outer membrane lipoprotein; Provisional

Protein Sequence : Show Sequence

>BAE44431.1 antigenic protein Et 18 [Edwardsiella tarda]
MTMRFPLTRCFPALVVLSALLLQGCVAAVIGSATMATQAASDPRSVGTQVDDGTLEARISNALSKDAQLK
KEARVVVTAYQGQVLLTGQAPSQALISRAKQIAMGVEGTKAVYNEIRLGQPVSLGTASADAWITTKVRSQ
LLASDQVKSTNVKVTTENGEVFLLGLVTPKEGQAAAQTASKVSGVKHVTTAFSLLK

Molecule Role : Protective antigen
Related Vaccine(s): E. tarda DNA vaccine pCE18

10. eta21

Gene Name : eta21
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : ACY64541
Other Database IDs : CDD:223685
Taxonomy ID : 636
Protein Name : putative peptidase
Protein pI : 5.08
Protein Weight : 53867.86
Protein Length : 571
Protein Note : Predicted Zn-dependent peptidase [General function prediction only]; COG0612

Protein Sequence : Show Sequence

>ACY64541.1 putative peptidase [Edwardsiella tarda]
MQYTKLRLMVGGLALAMVGTSVHSETLQPDPAWQEGKLPNGFSWQILATPQRPSDRIELRMIVNTGSLSE
SPQQVGYAHLLPRLALAQGKGLDATALQSFWQQAIDPVRPMPAAITSYDYTSYNLSLPNNRPDLMKDALR
WLANTAGDLQVNAETVGEALKGSDSRVMALPGEAGDPWWRYRLKGSNLLGHEPGRTAAVPLAPAQLKAFY
HQWYTPDAITLYVVGNVDARALSEQINQTFGPLSGKRETPATVPALPALAAAPVSLIADGAKQDILSLVW
DTPWQPIRDSQMLLRYWRSDLAREALFWHLQQVLANSALKGSVNLSFNCQVQYQRSQCAIHLETAQASLS
KTLAFIADEMSALRDDGITQPEFDTLLGQKNDQLSHLFATYARTNTDILMSQRLRSQQSGVVDIAPEAYQ
KLRQTFLSALTLDDINQELHIMLSREPAVVLRQPRGEAEENVGRLLEEYNRVMGLGGEEAVAAEAVDPPS
PPTAAENPAHETTPPQPLAQ

Molecule Role : Protective antigen
Molecule Role Annotation : (Jiao et al., 2009)

11. eta6

Gene Name : eta6
Sequence Strain (Species/Organism) : Edwardsiella tarda strain TX1
NCBI Protein GI : 262223754
Other Database IDs : CDD:320798
CDD:153074
Taxonomy ID : 636
Gene Strand (Orientation) : ?
Protein Name : hypothetical protein Eta6
Protein pI : 8.03
Protein Weight : 17712.21
Protein Length : 230
Protein Note : Protease Inhibitor Ecotin; homodimeric protease inhibitor; cl00178

Protein Sequence : Show Sequence

>ACY38270.1 hypothetical protein Eta6 [Edwardsiella tarda]
MKTFSMMMAGMMLAASSVSAWAGSADLSRQPLDKVAPYPQAEQGMTRQVIYLPPRDDEAALRVELLIGKT
LQVDCNRQRLMGQLESKTLQGWGYDYLVMDKVSGPMGTLMACPDNQRHPAFVTVHLGDSALQRYNSKLPL
VVYVPQGVQVKYRIWQAQPAVMDAQIK

Molecule Role : Protective antigen
Related Vaccine(s): E. tarda DNA vaccine pCE6

12. eta81

Gene Name : eta81
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : AFM47374
Taxonomy ID : 636
Protein Name : putative adhesin
Protein pI : 4.5
Protein Weight : 23166.97
Protein Length : 281

Protein Sequence : Show Sequence

>AFM47374.1 putative adhesin [Edwardsiella tarda]
MENNLLGDGFKLPKGLTDYGAAAQSWNQYAEKNGLTPEQKQAGLDRLAKGDLPEDTNITKAIVEGYQDGV
MIAETWYLGPAASVRKVIGGGIIAEIANGSYQWFDLSQPGNGNKSWDWKSSTSAGITGILAPRRSIGQNV
GIAMGSAFFTDGPDTGSIGGAAAGAWAGGLFGEYASGIVNSLTSKKVPGFIFNTTGSFSSEILGGYIKDA
VNGTQLSSERNKEVGE

Molecule Role : Protective antigen
Molecule Role Annotation : (Sun et al., 2012)

13. fimA

Gene Name : fimA
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : ABK57121
Other Database IDs : CDD:226069
Taxonomy ID : 636
Protein Name : major fimbrial subunit protein
Protein pI : 7.41
Protein Weight : 17441.13
Protein Length : 245
Protein Note : Pilin (type 1 fimbria component protein) [Cell motility]; COG3539

Protein Sequence : Show Sequence

>ABK57121.1 major fimbrial subunit protein [Edwardsiella tarda]
MKKILLPVMALSASVMSGQVSAANGTVNFTGEIINSTCQVSGDSKNIDVYLGKYPTSAFKAIGDKSASKA
FQINLEQCEPGTYTVRFDGNTVAGHPDLLAVSGDGSTAAAQGLGIEITDINGKHFAIANKATGEEPTVKV
VGDKKAVFNLQARYRSYADTVTAGLANATSPFTIEYK

Molecule Role : Protective antigen
Molecule Role Annotation : (Wang et al., 2013)

14. flagellar hook assembly protein FlgD

Gene Name : flagellar hook assembly protein FlgD
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : WP_035598485
Other Database IDs : CDD:235847
Taxonomy ID : 636
Gene Strand (Orientation) : ?
Protein Name : flagellar hook assembly protein FlgD
Protein pI : 4.31
Protein Weight : 22480.86
Protein Length : 311
Protein Note : flagellar basal body rod modification protein; Reviewed; PRK06655

Protein Sequence : Show Sequence

>WP_035598485.1 flagellar hook assembly protein FlgD [Edwardsiella tarda]
MAISVSLNDPVNTGSSVNASSKNGLVADNSQDLQNNFLTLLVAQLRNQDPTNPLQNNELTTQLAQISTVS
GIEKLNTTLGSISSQINSNQSLQASSLIGHGVMIPGKTILTGKGEGEGKVSTTPFGFELESGAQSVVVTI
TDNTGKVVKQIDMGSHNAGVFSYTWDGKGEDGVEVPDGAYNFSVSASNQGQQVVVQPLHFAMVNGVTQSS
KGALLELGLAGTATLADVRQIL

Molecule Role : Protective antigen
Molecule Role Annotation : (Beck et al., 2017)

15. flagellin FliC

Gene Name : flagellin FliC
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : ATI65365
Other Database IDs : CDD:235867
Taxonomy ID : 636
Protein Name : flagellin FliC
Protein pI : 4.68
Protein Weight : 42166.51
Protein Length : 479
Protein Note : flagellin; Validated

Protein Sequence : Show Sequence

>ATI65365.1 flagellin FliC [Edwardsiella tarda]
MAQVINTNSLSLMAQNNLNKSQSALGTAIERLSSGLRINSAKDDAAGQAISNRFTANINGLTQASRNAND
GISLAQTTEGALNEVNDNLQNIRRLTVQAQNGSNSSSDLQSIQDEINQRLSEIDRISQQTDFNGVKVLSS
DQKLTIQVGANDGETIDIDLKNINKDTLGLTSFSVAKGIDGTKATTALKAATNVDTGVSVDLAKTQTAKA
DSKTITGFVSTDDGAVYAINGDADKFFKVETVSKDGQVTVSATAAAGDELTAAKAGSTATKANITVAGSA
VTGADKGNLYEYTGTEKGHPQVVIKDGDKFYSASFDKDGKVTRGNELASVLKSEDPLATLDKALSQVDDL
RSGLGAVQNRFDSVINNLNSTVNNLSASRSRIQDADYATEVSNMSRAQILQQAGTSVLAQANQSTQNVLS
LLR

Molecule Role : Protective antigen
Molecule Role Annotation : (Jiao et al., 2010)

16. fliC

Gene Name : fliC
Sequence Strain (Species/Organism) : Edwardsiella tarda strain TX1
NCBI Protein GI : 262223752
Other Database IDs : CDD:235867
CDD:279061
CDD:279090
Taxonomy ID : 636
Gene Strand (Orientation) : ?
Protein Name : flagellin
Protein pI : 4.71
Protein Weight : 41122.32
Protein Length : 467
Protein Note : flagellin; Validated

Protein Sequence : Show Sequence

>ACY38269.1 flagellin [Edwardsiella tarda]
MAQVINTNSLSLMAQNNLNKSQSALGTAIERLSSGLRINSAKDDAAGQAISNRFTANINGLTQASRNAND
GISLAQATEGALNEVNDNLQNIRRLTVQAQNGSNSSSDLQSIQDEITQRLSEIDRISQQTDFNGVKVLSK
DQKLTIQVGANDGETIDIDLKNINAQSLGLDKFNVADSVDTTKVAAAAATKVNGPMVNIDTNGKDAGGKA
ITGYVKDDKGDVYGAYDDAGTTKYAKATIGDDGKVTAFDTASATPSGTTNAVSQVTSKVAPKGADAANVY
AYTGTEKGASAYVIKEGTGADAKYFKASVAEDGTVTKGSALSTTAKTADPLATLDKALSQVDDLRSGLGA
VQNRFDSVINNLNSTVNNLSASRSRIQDADYATEVSNMSRAQILQQAGTSVLAQANQSTQNVLSLLR

Molecule Role : Protective antigen
Related Vaccine(s): E. tarda DNA vaccine pCE18 , E. tarda DNA vaccine pCE6

17. GAPDH

Gene Name : GAPDH
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : AGO59148
Other Database IDs : CDD:185323
CDD:278473
CDD:280894
Taxonomy ID : 636
Protein Name : glyceraldehyde-3-phosphate dehydrogenase
Protein pI : 6.52
Protein Weight : 32379.9
Protein Length : 411
Protein Note : glyceraldehyde-3-phosphate dehydrogenase A; Provisional; PRK15425

Protein Sequence : Show Sequence

>AGO59148.1 glyceraldehyde-3-phosphate dehydrogenase [Edwardsiella tarda]
MTIKVGINGFGRIGRIVFRAAQERCDIEIVGINDLLDANYMAYMLKYDSTHGRFNGTVEVKDGHLIVNGK
KIRVTAERDPANLKWDEIGVDVVAEATGLFLTDETARKHIAAGAKKVVMTGPSKDATPMFVMGVNHKNYA
GQEIVSNASCTTNCLAPLAKVLNDNFGIVEALMTTVHATTATQKTVDGPSMKDWRGGRGASQNIIPSSTG
AAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTARLAKPATYQQICDVMKAASEGEMKGVLGYTDEAVV
STDFNGEVCTSVFDADAGISLNDNFVKLVSWYDNETGYSNKVLDLIAHISK

Molecule Role : Protective antigen
Molecule Role Annotation : (Trung et al., 2014)

18. groEL

Gene Name : groEL
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : AGN29187
Other Database IDs : CDD:295468
Taxonomy ID : 636
Protein Name : GroEL
Protein pI : 4.4
Protein Weight : 35715.74
Protein Length : 421
Protein Note : chaperonin_like superfamily. Chaperonins are involved in productive folding of proteins. They share a common general morphology, a double toroid of 2 stacked rings, each composed of 7-9 subunits. There are 2 main chaperonin groups. The symmetry of type I...; cl02777

Protein Sequence : Show Sequence

>AGN29187.1 GroEL, partial [Edwardsiella tarda]
VLDKSFGSPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNEGLKAVA
AGMNPMDLKRGIDKAVIAAVEELKALSVPCSDSKAIAQVGTISANADDTVGQLIAEAMEKVGKEGVITVE
EGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLL
IIAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIATLTAGTVISEEIGMELEKATLEDLG
QAKRVVINKDTTTIIDGVGEESAIQGRVTQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVE
MKEKKARVEDALHATR

Molecule Role : Protective antigen
Molecule Role Annotation : (Liu et al., 2016)

19. invasin

Gene Name : invasin
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : AKH88306
Other Database IDs : CDD:288753
CDD:214751
Taxonomy ID : 636
Protein Name : invasin
Protein pI : 6.4
Protein Weight : 73137.11
Protein Length : 746
Protein Note : Invasin beta-domain of outer membrane; pfam11924

Protein Sequence : Show Sequence

>AKH88306.1 invasin [Edwardsiella tarda]
MLTWVNVICQILSPISMTFTPVIVARAEVPPRPVYTSTQPYILRNDDSIASIAAKFNTTPAQLEKLNQFR
HFAHGFMQVGPGDEIDVPNPAMEKRRQHDEESAAQRSRVDSSRRETWLQPPAPLMQAGMALASSHPSEAT
TSLARTLATSAANEEIQTWLKQYGTARVQLNVDKNFSLQESALDWLLPLSDTPNLTLFTQLGARNKDHRH
TMNIGVGARTLFGNWLFGLNTFYDRDLTGHNSRIGMGAEAWTDYLQLSANSYFRLGSWHQSHDFADYDER
AANGFDLRANGWLPALPQLGAKLVYEQYQGEHVALFGKDNLQRNPSALTAGINYTPFPLLTIGIDERLGK
EGHNDTQFNIQLNYHPGAEWQSQIDPGAVAATRRIDEARYNLVERNNNIVLEYKKQDLIHLALSSHAIRA
WPGSVHTLSAILQSKYGIEQISWQDGGLAAAGGQLIALDKTHFRLTLPPYKVARARNAAPTPTAEEIAAN
TYHLTAIAFDSQGNPSNSETLVVTVQPLQAVLRSAPIVTGDYALANGRDQIGVDFIVQDSQGNPLVDREV
ILHTNNGAQPTTLTVKTDSRGIAHIAVTNTNAGITRVTAEIDGQTQYQDITFVHPDISITISGLVKGYPL
VGETLSAQVSCGTAGCPNALRYQWQIEDAINSGRYSDIPGANATTYTPIGSDQKRRIQVIVNPE

Molecule Role : Protective antigen
Molecule Role Annotation : (Li et al., 2012)

20. nanA

Gene Name : nanA
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : AFP73415
Other Database IDs : CDD:271234
CDD:214872
Taxonomy ID : 636
Protein Name : NanA
Protein pI : 5.17
Protein Weight : 71114.69
Protein Length : 719
Protein Note : Non-viral sialidases; cd15482

Protein Sequence : Show Sequence

>AFP73415.1 NanA [Edwardsiella tarda]
MLIFAEHDRTANNGWRIPAITVTQGGALIAVSDARAVGTSDIGQNQDVHFGYRISYDRGDTWGEIHDIAP
SFNGKRQISDPAIVYNPDSGSTFLFGYYNDRFITSKPLSRVSDFFMFTSDDGGKTWDRGTSLYDLAPTGY
KYILQGPGSGMYHDGTLYLAAQAWHHDRDSSLVGSGATVTSGYLYSSDNGVTWNSAWLRPDDKIVGAPGT
GVDDLPDITSESSVFHHDGHIYLAAKSETSRESKSRVVYRTKDNGQRWERVEEAFLPENISRAETSSLSL
DDSVYLVGYSTATERANRDGIYITTNTGRTIQVYDSPSYGYTSMTKDEDNLYILFEGEGDIYLQRYDIAA
RDYANISATILNRSNDLFDIATKLRTASSYIKGGYGSHASSDAEFVYADDWGKLGVFFARQSENSQRVPG
TIQYTNSDVSLTLAKDDILFSGDNVFVGYQHSDIQYDNDAANKVKSVLAGYSYQHDFGVLSYHLMVNTIY
GENRFSRNRREGLGRKADFNAYSVAIKNMLAKDIYLADGFYAGVASGLHSTFFSHSAIQERGGNGWNNAS
VDSADHASNQLLLDGQLSKRLHIMQSNELTLTAKASYQYELMDTAKWAEAYRVLDARRLMPAPVKRYPHG
LATGYLEAALSLHPQAVIAAGASLNTDGDGLVAGSFTYTF

Molecule Role : Protective antigen
Molecule Role Annotation : (Jin et al., 2012)

21. ompA

Gene Name : ompA
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : ACT33424
Other Database IDs : CDD:236764
CDD:279698
CDD:143586
Taxonomy ID : 636
Protein Name : outer membrane protein A precursor
Protein pI : 7.48
Protein Weight : 35556.31
Protein Length : 426
Protein Note : outer membrane protein A; Reviewed

Protein Sequence : Show Sequence

>ACT33424.1 outer membrane protein A precursor [Edwardsiella tarda]
MKKTAIALAVALAGFATVAQAAPKDDTWYVGGKLGWSHFISNSFEDMGTTKSHPNQLGAGAFFGYQANPY
LGFEMGYDWLGRMGYTGDVNAKFKSQGVQLAAKLSYPLMDDLDVYTRLGGMVWRSDIHGAADGGDYTSSH
DTGVSPLAAIGVEYALNKDWATRLDYQYVNKVGTRSETGARPDNTMLSLGVVYRFGQDEVAAPAPIPAPA
PAPVVETKRFTLKSDVLFNFNKYTLKAEGRQALDQLYSQLSSMDPKDGSVVVLGYTDRIGSDQYNLKLSK
QRAQTVVDYLVSKGIPADKIAARGMGKADPVTGSTCDNVKPRAALINCLAPDRRVVIEVKGIKEEVTQPQ
A

Molecule Role : Protective antigen
Molecule Role Annotation : (Beck et al., 2017)

22. ompC

Gene Name : ompC
Sequence Strain (Species/Organism) : Edwardsiella tarda
NCBI Protein GI : ADM43235
Other Database IDs : CDD:328747
CDD:238208
Taxonomy ID : 718251
Protein Name : Outer membrane protein C precursor Porin OmpC
Protein pI : 8.23
Protein Weight : 40772.04
Protein Length : 470
Protein Note : Porin superfamily. These outer membrane channels share a beta-barrel structure that differ in strand and shear number. Classical (gram-negative) porins are non-specific channels for small hydrophillic molecules and form 16 beta-stranded barrels (16,20)...; cl21487

Protein Sequence : Show Sequence

>ADM43235.1 Outer membrane protein C precursor Porin OmpC [Edwardsiella tarda FL6-60]
MMNKIRSLVALSTIGCIGIFPLISHAAEIYNQNGNRVYLDGSFKVRHYFSQDKYIAGDQSKVKFTLRGET
KINDLMIGYARWEYNVKLNQPESAGSTKNTTRIGYAGVGFGQYGSFDYGRNYGILNDINGWTGAPIPVFG
GVSYDGVDNFMTYRTNNVATYRNRNIFNLVDGLDFGLQVQGKNDGWNDPENQTGPLSSNPRGVAHQNGNG
VGTSLVYRFENGLSVGGAYANSARTLEQRQDDLGKRADGWNVGARYDNHNVYLAAIYGEVKNMHYIGKQD
GFAPKARGYELLAQYQFDCGLKPSIAYLNGTAKDLKGNAGSNQTYVKFIDLATTYSFNKNLALIFEYKIN
LLDDNTFTRNNGISTDDVFVTMLNYKF

Molecule Role : Protective antigen
Molecule Role Annotation : (Liu et al., 2017)

23. ompX

Gene Name : ompX
Sequence Strain (Species/Organism) : Edwardsiella tarda ATCC 15947
NCBI Protein GI : GAC64620
Taxonomy ID : 667121
Protein Name : outer membrane protein X
Protein pI : 8.81
Protein Weight : 20276.732
Protein Length : 277
Protein Note : contig: ET112;
type strain of Edwardsiella tarda

Protein Sequence : Show Sequence

>GAC64620.1 outer membrane protein X [Edwardsiella tarda ATCC 15947 = NBRC 105688]
MKTRWRALSLLITMGITAPAMADAQTVSLGYAQAKVADFKNIAGVNLKYRYEWDSPLSLMTSFTYMSGSK
SFEDKAGLDYSRGNGDVKYYSLAAGPAWRISDTVSVYGLVGVNVNKTTVSSTWREVSFGSYQEGSLQESR
SKGAVMYGAGVQINPLANWSVDIGYEGSRVDLGGSKHSINGFNLGVGYRF

Molecule Role : Protective antigen
Molecule Role Annotation : (Liu et al., 2017)

24. putative exoprotein-precursor

Gene Name : putative exoprotein-precursor
Sequence Strain (Species/Organism) : Edwardsiella tarda EIB202
NCBI Protein GI : ACY84923
Other Database IDs : CDD:328937
Taxonomy ID : 498217
Protein Name : putative exoprotein-precursor
Protein pI : 4.89
Protein Weight : 29144.23
Protein Length : 349
Protein Note : Autotransporter beta-domain; cl22877

Protein Sequence : Show Sequence

>ACY84923.1 putative exoprotein-precursor [Edwardsiella tarda EIB202]
MGFSEVMPALITREADRQLDWVLIGFDVAPDSEREEDAASLLALNYRSFMAEVQYLDKRQLDLRNDPTDS
GVWARVLHGAGSAAENYRDRYLHLQVGADRRYGLGETGDLFAGATATFTDGRASGDAFSSKSRALGAGLY
ATALFPSGLFVDLNGKYVHHSTEYQVSLAGLGERKATNHAWYARADVGYRYGLSESAFVEPHLEMVYGTY
SSTRFDWRDQGMAVSLAQPRFTPLLATAGVALGKQFTLGEWQTTLRAGVDYQRELISGGGYAAA

Molecule Role : Protective antigen
Molecule Role Annotation : (Song et al., 2013)

25. putative flagellin associated protein

Gene Name : putative flagellin associated protein
Sequence Strain (Species/Organism) : Edwardsiella tarda EIB203
NCBI Protein GI : ACY84965
Other Database IDs : CDD:235867
Taxonomy ID : 498217
Protein Name : putative flagellin associated protein
Protein pI : 4.67
Protein Weight : 41788.22
Protein Length : 501
Protein Note : flagellin; Validated

Protein Sequence : Show Sequence

>ACY84965.1 putative flagellin associated protein [Edwardsiella tarda EIB202]
MAQVINTNSLSLMAQNNLNKSQSALGTAIERLSSGLRINSAKDDAAGQAISNRFTANINGLTQASRNAND
GISLAQTTEGALNEVNDNLQNIRRLTVQAQNGSNSSSDLQSIQDEITQRLSEIDRISQQTDFNGVKVLSK
DQKLTIQVGANDGETIDIDLKNINAQSLGLDKFNVADSVDTTKVAAAAPAKVKTNIDVAINNDATLKADS
KDITGYEQKGADLYAKNTDGKLFKVTTIDNTTGKVTGVDTTEYTGGGTTAVTSIKKEVAPTGPDAANLRA
YSGIEKGASAYVIQEGTGADAKYFKASVADDGTVTKGSALSTTVKTADPLATLDKALSQVDDLRSGLGAV
QNRFDSVINNLNSTVNNLSASRSRIQDADYATEVSNMSRAQILQQAGTSVLAQANQSTQNVLSLLR

Molecule Role : Protective antigen
Molecule Role Annotation : (Song et al., 2013)

26. slyA

Gene Name : slyA
Sequence Strain (Species/Organism) : Edwardsiella tarda EIB202
NCBI Gene ID : 8608031
NCBI Protein GI : 269139033
Locus Tag : ETAE_1684
Genbank Accession : CP001135
Protein Accession : YP_003295734
Taxonomy ID : 498217
Gene Starting Position : 1791955
Gene Ending Position : 1792332
Gene Strand (Orientation) : +
Protein Name : transcriptional regulator
Protein pI : 6.52
Protein Weight : 13582.65
Protein Length : 125

DNA Sequence : Show Sequence

>gi|269137358:1791955-1792332 Edwardsiella tarda EIB202 chromosome, complete genome
CTTGATCGATCAGCGACTGAAGCCGCTGGAGCTGACGCAGACGCATTGGGTGACGCTGTATAACATTCAC
CGCCTGCCGCCGGAGCAGTCACAGATTCAGCTGGCCAAGGCGATTGGCATTGAGCAGCCGTCGCTGGTGC
GTACGCTGGATCAGCTGGAAGATAAGGGTCTGATTACTCGACATATCTGTGCTAACGATCGCCGTGCCAA
ACGCATTAAGCTGACGGACGGTGCCGAACCCGTGATTAGGGAAGTGACCGACGTGATTTCGCTGACGCGC
AGTGAAATCCTGGACGGTATCTCAGCGGATGAGGTCTCGCTGTTGACCAGTCTGGTCGAACGTCTGGAGC
AGAACATTATTCATTTACAGAATAAATA

Protein Sequence : Show Sequence

>gi|269139033|ref|YP_003295734.1| transcriptional regulator [Edwardsiella tarda EIB202]
MIDQRLKPLELTQTHWVTLYNIHRLPPEQSQIQLAKAIGIEQPSLVRTLDQLEDKGLITRHICANDRRAK
RIKLTDGAEPVIREVTDVISLTRSEILDGISADEVSLLTSLVERLEQNIIHLQNK

Molecule Role : Virmugen
Molecule Role Annotation : An aroC and slyA mutant is attenuated in zebra fish. This mutant is also protective against challenge from wild type E. tarda, resulting in a relative percent survival of 80.1% (Xiao et al., 2011).
Related Vaccine(s): Edwardsiella tarda aroC/slyA mutant vaccine

III. Vaccine Information

1. BL21(DE3)/pUTa-E + pET28a-FT

a. Vaccine Ontology ID:

VO_0004804

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Zebrafish

e. Preparation

Cell-penetrating peptides (CPPs) are short cationic/amphipathic peptides which facilitate cellular uptake of various molecular cargoes and therefore have great potentials in vector vaccine design. Among the tested CPPs, TAT showed an excellent capability to deliver the cargo protein EGFP into cytoplasm (Ma et al., 2014). In order to establish an efficient antigen delivery system in Escherichia coli, the EGFP-TAT synthesis circuit was combined with an in vivo inducible lysis circuit PviuA-E in E. coli to form an integrated antigen delivery system, the resultant E. coli was proved to be able to lyse upon the induction of a mimic in vivo signal and thus release intracellular EGFP-TAT intensively, which were assumed to undergo a more efficient intracellular delivery by CPP to evoke protective immune responses. Based on the established antigen delivery system, the protective antigen gene flgD from an invasive intracellular fish pathogen Edwardsiella tarda EIB202, was applied to establish an E. coli recombinant vector vaccine (Ma et al., 2014).

f. Immunization Route

Intramuscular injection (i.m.)

g. Zebrafish Response

Vaccine Immune Response Type: VO_0000287
Efficacy: BL21(DE3)/pUTa-E + pET28a-FT conferred an significantly better immune protection (RPS of 63%) than the other control strains. This result suggested fused cell-penetrating peptides (CPPs) could facilitate the internalization of antigens, released by the lysed bacteria, into the immune-related cells, such as macrophage, and in turn promote a more protective immune response in host (Ma et al., 2014).

2. E. coli-CPPs (Edwardsiella tarda)

a. Vaccine Ontology ID:

VO_0004640

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Preparation

GFP-TAT synthesis circuit was combined with an in vivo inducible lysis circuit PviuA-E in E. coli to form an integrated antigen delivery system (Ma et al., 2014).

f. Immunization Route

Intramuscular injection (i.m.)

g. Fish Response

Vaccine Immune Response Type: VO_0003057
Challenge Protocol: Hosts were challenged with E. tarda EIB202 (Ma et al., 2014).
Efficacy: This E. coli vector vaccine presented superior immune protection under the challenge with E. tarda EIB202, suggesting that the novel antigen delivery system had great potential in bacterial vector vaccine applications (Ma et al., 2014).

3. E. tarda DNA vaccine pCE18

a. Vaccine Ontology ID:

VO_0004556

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Japanese flounder

e. Antigen

FliC-Et18 fusion protein from E. tarda TX1 (Jiao et al., 2009)

f. Gene Engineering of et18

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

g. Gene Engineering of fliC

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

h. Vector:

pCN3 (Jiao et al., 2009)

i. Immunization Route

Intramuscular injection (i.m.)

j. Fish Response

Vaccine Immune Response Type: VO_0003057
Efficacy: Following TX1 challenge, fish immunized with pCE18, pCN3 (plasmid), and PBS exhibited cumulative mortalities of 35%, 90%, and 95%, respectively. Hence, with pCN3 as a control, the protective efficacy of pCE18 was 61% in terms of RPS (Jiao et al., 2009).

4. E. tarda DNA vaccine pCE6

a. Vaccine Ontology ID:

VO_0004557

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Japanese flounder

e. Antigen

FliC-Eta6 fusion protein from E. tarda TX1 (Jiao et al., 2009)

f. Gene Engineering of fliC

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

g. Gene Engineering of eta6

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

h. Vector:

pCN3 (Jiao et al., 2009)

i. Immunization Route

Intramuscular injection (i.m.)

j. Fish Response

Vaccine Immune Response Type: VO_0003057
Efficacy: Upon exposure to TX1 challenge, the cumulative mortalities of the pCE6-, pCN3-, and PBS-vaccinated fish were 25%, 90%, and 95%, respectively. Therefore, compared to vaccination with pCN3, vaccination with pCE6 produced a RPS of 72%, which is significantly higher than that produced by pEta6 (Jiao et al., 2009).

5. E. tarda DNA vaccine pCEsa1

a. Vaccine Ontology ID:

VO_0004558

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Japanese flounder

e. Antigen

Esa1 from E. tarda TX1 (Sun et al., 2011)

f. Gene Engineering of esa1

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

g. Vector:

pCN3 (Sun et al., 2011)

h. Immunization Route

Intramuscular injection (i.m.)

i. Fish Response

Vaccine Immune Response Type: VO_0003057
Efficacy: The accumulated mortalities of pCEsa1-, pCN3-, and PBS-vaccinated fish were 20%, 76%, and 80%, respectively. Hence, compared to PBS-vaccinated fish, pCEsa1-vaccinated fish were significantly (P < 0.05) protected, with a RPS of 75% (Sun et al., 2011).

6. Edwardsiella tarda aroC mutant vaccine

a. Vaccine Ontology ID:

VO_0002830

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Zebra fish

e. Gene Engineering of aroC

Type: Gene mutation
Description: This aroC mutant is from Edwardsiella tarda (Xiao et al., 2011)
Detailed Gene Information: Click here.

f. Immunization Route

Intramuscular injection (i.m.)

g. Fish Response

Persistence: An aroC mutant is attenuated in zebra fish (Xiao et al., 2011).
Efficacy: An aroC mutant is protective in zebra fish against challenge from wild type E. tarda, resulting in a 68.3% relative percent survival (Xiao et al., 2011).

7. Edwardsiella tarda aroC/esrC mutant vaccine

a. Type:

Live, attenuated vaccine

b. Status:

Research

c. Host Species as Laboratory Animal Model:

Zebra fish

d. Gene Engineering of aroC

Type: Gene mutation
Description: This aroC/esrC mutant is from Edwardsiella tarda (Xiao et al., 2011).
Detailed Gene Information: Click here.

e. Gene Engineering of esrC

Type: Gene mutation
Description: This aroC/esrC mutant is from Edwardsiella tarda (Xiao et al., 2011).
Detailed Gene Information: Click here.

f. Immunization Route

Intramuscular injection (i.m.)

g. Fish Response

Persistence: This aroC/esrC mutant is attenuated in zebra fish (Xiao et al., 2011).
Efficacy: This aroC/esrC mutant is protective against challenge from wild type E. tarda, with a 71.3% relative percent survival (Xiao et al., 2011).

8. Edwardsiella tarda aroC/slyA mutant vaccine

a. Type:

Live, attenuated vaccine

b. Status:

Research

c. Host Species as Laboratory Animal Model:

Zebra fish

d. Gene Engineering of slyA

Type: Gene mutation
Description: An aroC and slyA mutant is attenuated in zebra fish. This mutant is also protective against challenge from wild type E. tarda, resulting in a relative percent survival of 80.1% (Xiao et al., 2011).
Detailed Gene Information: Click here.

e. Immunization Route

Intramuscular injection (i.m.)

f. Fish Response

Persistence: This aroC/slyA mutant is attenuated in zebra fish (Xiao et al., 2011).
Efficacy: This aroC/slyA mutant is protective in zebra fish against challenge with wild type E. tarda. The percent relative survival is 80.1% (Xiao et al., 2011).

9. Edwardsiella tarda esrB mutant vaccine

a. Vaccine Ontology ID:

VO_0002833

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Fish

e. Gene Engineering of esrB

Type: Gene mutation
Description: This esrB mutant is from Edwardsiella tarda (Lan et al., 2007).
Detailed Gene Information: Click here.

f. Immunization Route

Intraperitoneal injection (i.p.)

g. Fish Response

Persistence: A esrB mutant is attenuated in fish (Lan et al., 2007).
Efficacy: An esrB mutant induces significant protection in fish from challenge with wild type Edwardsiella tarda (Lan et al., 2007).

IV. References

1. Beck et al., 2017: Beck BR, Lee SH, Kim D, Park JH, Lee HK, Kwon SS, Lee KH, Lee JI, Song SK. A Lactococcus lactis BFE920 feed vaccine expressing a fusion protein composed of the OmpA and FlgD antigens from Edwardsiella tarda was significantly better at protecting olive flounder (Paralichthys olivaceus) from edwardsiellosis than single antigen vaccines. Fish & shellfish immunology. 2017; 68; 19-28. [PubMed: 28687358].

2. Hu et al., 2012: Hu YH, Dang W, Deng T, Sun L. Edwardsiella tarda DnaK: expression, activity, and the basis for the construction of a bivalent live vaccine against E. tarda and Streptococcus iniae. Fish & shellfish immunology. 2012; 32(4); 616-620. [PubMed: 22281608].

3. Jiao et al., 2009: Jiao XD, Zhang M, Hu YH, Sun L. Construction and evaluation of DNA vaccines encoding Edwardsiella tarda antigens. Vaccine. 2009; 27(38); 5195-5202. [PubMed: 19596416].

4. Jiao et al., 2009: Jiao XD, Dang W, Hu YH, Sun L. Identification and immunoprotective analysis of an in vivo-induced Edwardsiella tarda antigen. Fish & shellfish immunology. 2009; 27(5); 633-638. [PubMed: 19706328].

5. Jiao et al., 2010: Jiao XD, Zhang M, Cheng S, Sun L. Analysis of Edwardsiella tarda DegP, a serine protease and a protective immunogen. Fish & shellfish immunology. 2010; 28(4); 672-677. [PubMed: 20060910].

6. Jiao et al., 2010: Jiao XD, Hu YH, Sun L. Dissection and localization of the immunostimulating domain of Edwardsiella tarda FliC. Vaccine. 2010; 28(34); 5635-5640. [PubMed: 20580470].

7. Jin et al., 2012: Jin RP, Hu YH, Sun BG, Zhang XH, Sun L. Edwardsiella tarda sialidase: pathogenicity involvement and vaccine potential. Fish & shellfish immunology. 2012; 33(3); 514-521. [PubMed: 22705341].

8. Lan et al., 2007: Lan MZ, Peng X, Xiang MY, Xia ZY, Bo W, Jie L, Li XY, Jun ZP. Construction and characterization of a live, attenuated esrB mutant of Edwardsiella tarda and its potential as a vaccine against the haemorrhagic septicaemia in turbot, Scophthamus maximus (L.). Fish & shellfish immunology. 2007; 23(3); 521-530. [PubMed: 17478097].

9. Li et al., 2012: Li MF, Hu YH, Zheng WJ, Sun BG, Wang CL, Sun L. Inv1: an Edwardsiella tarda invasin and a protective immunogen that is required for host infection. Fish & shellfish immunology. 2012; 32(4); 586-592. [PubMed: 22289712].

10. Liu et al., 2016: Liu F, Tang X, Sheng X, Xing J, Zhan W. DNA vaccine encoding molecular chaperone GroEL of Edwardsiella tarda confers protective efficacy against edwardsiellosis. Molecular immunology. 2016; 79; 55-65. [PubMed: 27701022].

11. Liu et al., 2017: Liu F, Tang X, Sheng X, Xing J, Zhan W. Comparative study of the vaccine potential of six outer membrane proteins of Edwardsiella tarda and the immune responses of flounder (Paralichthys olivaceus) after vaccination. Veterinary immunology and immunopathology. 2017; 185; 38-47. [PubMed: 28242001].

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