VIOLIN: Vaccine Investigation and Online Information Network

Haemophilus parasuis

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. csgG (Protective antigen)
2. FOG (Protective antigen)
3. gapA (Protective antigen)
4. gcp (Protective antigen)
5. hsdS (Protective antigen)
6. hxuB (Protective antigen)
7. hxuC (Protective antigen)
8. LppC (Protective antigen)
9. ndk (Protective antigen)
10. omp26 (Protective antigen)
11. oppA (Protective antigen)
12. outer membrane protein A (Protective antigen)
13. outer membrane protein D15 (Protective antigen)
14. outer membrane protein P2 (Protective antigen)
15. palA (Protective antigen)
16. pflA (Protective antigen)
17. plpA (Protective antigen)
18. rnfC (Protective antigen)
19. superoxide dismutase (Protective antigen)
20. tbpA (Protective antigen)
21. tbpB (Protective antigen)
22. TolC (Protective antigen)
23. VtaA5 (Protective antigen)
24. VtaA6 (Protective antigen)
25. VtaA8 (Protective antigen)
26. VtaA9 (Protective antigen)
27. yfeA (Protective antigen)
28. ygiW (Protective antigen)
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

738

2. Disease:

Glasser's disease

3. Introduction

H. parasuis is a non-motile, small, pleomorphic rod in the family Pasteurellaceae, and is the etiologic agent of Glasser's disease. Glasser’s disease is characterized by fibrinous polyserositis, polyarthritis and meningitis. H. parasuis often produces acute septicemia, and its endotoxin induces disseminated intravascular coagulation, resulting in the formation of microthrombi in several organs (Oliveira and Pijoan, 2004).

4. Microbial Pathogenesis

H. parasuis colonizes the respiratory tracts of pigs, and can then cause systemic infection (Oliveira and Pijoan, 2004).

5. Host Ranges and Animal Models

Pigs

6. Host Protective Immunity

Early colonized pigs are protected against systemic infection by maternal antibodies. As maternal immunity decreases, these pigs are able to mount an active immune response to these virulent strains (Oliveira and Pijoan, 2004).

II. Vaccine Related Pathogen Genes

1. csgG

Gene Name : csgG
Sequence Strain (Species/Organism) : Haemophilus parasuis SH0165
NCBI Protein GI : ACL33081
Other Database IDs : CDD:224379
Taxonomy ID : 557723
Protein Name : putative lipoprotein, curli production assembly/transport component CsgG
Protein pI : 8.61
Protein Weight : 22023.72
Protein Length : 345
Protein Note : Curli biogenesis system outer membrane secretion channel CsgG [Cell wall/membrane/envelope biogenesis]; COG1462

Protein Sequence : Show Sequence

>ACL33081.1 putative lipoprotein, curli production assembly/transport component CsgG [Glaesserella parasuis SH0165]
MSNLKVGVLCFTVLTLVGCATEGSRVITPTSTQAAITASQYKGVKSVISVGKFDNKSSFQNGVFSDGADQ
LGNQSKTILISHLQQTGRFSVVDRTNMSEIASEAKIKGKKQNLKGATYVITGDVTEFGRKTVGDHQLFGI
LGRGKEQVAYAKVTLNVVNVETSEVVYSSQGAGEYNLSNREVLGFGGTASYDATLNGKVLDLAIREAVNS
LVNGIESGSWQPSH

Molecule Role : Protective antigen
Molecule Role Annotation : (Hong et al., 2011)

2. FOG

Gene Name : FOG
Sequence Strain (Species/Organism) : Haemophilus parasuis SH0165
NCBI Protein GI : ACL32772
Other Database IDs : CDD:276809
CDD:290160
CDD:285020
CDD:304382
Taxonomy ID : 557723
Protein Name : possible outer membrane protein, FOG: TPR repeat protein
Protein pI : 9.94
Protein Weight : 54014.03
Protein Length : 584
Protein Note : putative protein binding surface [polypeptide binding]

Protein Sequence : Show Sequence

>ACL32772.1 possible outer membrane protein, FOG: TPR repeat protein [Glaesserella parasuis SH0165]
MPDLHFMKSSQFPFILLFVSPILLANPFADPVSFERLSQAVGFEQKFAKKSADLTASPKAVLSLPNPQSL
EAQINQAIIEQQWQRLPTLLQAYQQQSHYDITLVHYALGAFYYGQQRYPEAIEHYQAVLAQQPTLAYPRF
DLGVMLYENQQYRQATEQLTMAKANLAPSMQPLTERYLARMVEEQSWQPDFSLQYVRTDNVNNASSSPVV
NINGRQFVKDKTALPQSAEGFRYGIGVSKAHNLAGNHYLNGSVHYSGVHYWDNQDYSEQSLRFSLGYQYR
NAKMGLGVTPFIEQNWLGSPRYSRQFGATLHGYRYLTQKWIVSSRLTHNQKRYHDSLTASRYNGFQNSGA
LTLRWQAVRSWQIFASIEANRDQTKEKASSSHKIGGTLGVIYRWKNWGSELSGGYAKRNFEGKHYLYGYK
RVDNEYRANWSLWNKQWTWKGLMPKLNFSYVKIQSNIPDFYSRKTGEWFLTVEKRF

Molecule Role : Protective antigen
Molecule Role Annotation : (Yuan et al., 2012)

3. gapA

Gene Name : gapA
Sequence Strain (Species/Organism) : Haemophilus parasuis SH0165
NCBI Gene ID : 7278596
NCBI Protein GI : 498484148
Locus Tag : HAPS_RS00005
Genbank Accession : CP001321
Protein Accession : WP_010785946
Taxonomy ID : 557723
Gene Starting Position : 1
Gene Ending Position : 1020
Gene Strand (Orientation) : +
Protein Name : type I glyceraldehyde-3-phosphate dehydrogenase
Protein pI : 6.07
Protein Weight : 32717.08
Protein Length : 339
Protein Note : COG:G;COG0057

DNA Sequence : Show Sequence

>NC_011852.1:1-1020 Haemophilus parasuis SH0165, complete genome
TATGGCAATTAAAATTGGTATTAACGGCTTTGGCCGTATCGGTCGTATCGTATTCCGTGCAGCACAAAAA
CGTGATGACATCGAAGTAGTTGGTATCAACGACTTAATTGATGTTGAGTATATGGCATATATGCTCAAAT
ACGATTCAACTCACGGTCGTTTCGATGGCACTGTCGAAGTAAAAGACGGCAAATTAGTTGTAAACGGTAA
AGAAATCCGTGTTACTTCTGAGCGTGACCCAGCTAACTTAAAATGGAATGAAATTGGTGTGGATATCGCA
GTTGAAGCAACAGGTTTATTCTTGGACGATACAACTGCTCGTAAACACATCGAAGCTGGTGCGAAAAAAG
TCGTTTTAACTGGTCCATCTAAAGATAGCACACCTATGTTCGTAAACGGTGTAAACTTCAACGCTTATGC
TGGTCAAGACATCGTTTCTAACGCTTCTTGTACAACAAACTGCTTAGCACCACTTGCTCGTGTTATCCAC
GAAACATTTGGTATCAAAGAAGGTTTAATGACAACTGTTCATGCAACAACAGCAACACAAAAAACTGTTG
ATGGTCCATCAGCAAAAGACTGGCGCGGTGGTCGTGGTGCGGCACAAAACATCATTCCATCTTCAACAGG
TGCAGCGAAAGCAGTAGGTAAAGTATTACCAGCATTAAACGGTAAATTAACAGGTATGGCGTTCCGTGTT
CCAACAGCAAACGTATCTGTTGTTGATTTAACTGTAAACTTAGAAAAACCAGCAAGCTACGAAGAGATCA
AAAAAGCGATCAAAGATGCAGCAGAAGGTAAAACATTCAACGGCGAATTAAAAGGCGTTTTAGGTTATAC
TGAAGATGCAGTAGTATCAACAGACTTCAACGGTGCAGTTGAAACTTCTGTATTTGATGCAGATGCAGGT
ATCGCATTAACAGATACATTCGTGAAACTTGTTTCTTGGTATGACAACGAAACTGGTTACTCAAACAAAG
TATTAGACTTAGTTGCTCACGTTTACAACTACAAAGGCTA

Protein Sequence : Show Sequence

>WP_010785946.1 type I glyceraldehyde-3-phosphate dehydrogenase [Glaesserella parasuis]
MAIKIGINGFGRIGRIVFRAAQKRDDIEVVGINDLIDVEYMAYMLKYDSTHGRFDGTVEVKDGKLVVNGK
EIRVTSERDPANLKWNEIGVDIAVEATGLFLDDTTARKHIEAGAKKVVLTGPSKDSTPMFVNGVNFNAYA
GQDIVSNASCTTNCLAPLARVIHETFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGAAQNIIPSSTG
AAKAVGKVLPALNGKLTGMAFRVPTANVSVVDLTVNLEKPASYEEIKKAIKDAAEGKTFNGELKGVLGYT
EDAVVSTDFNGAVETSVFDADAGIALTDTFVKLVSWYDNETGYSNKVLDLVAHVYNYKG

Molecule Role : Protective antigen
Related Vaccine(s): Haemophilus Parasuis Avirulent Live Culture Vaccine (USDA: 19J1.00) , pCgap DNA vaccine encoding glyceraldehyde-3-phosphate dehydrogenase

4. gcp

Gene Name : gcp
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : EQA95212
Taxonomy ID : 456298
Protein Name : putative tRNA threonylcarbamoyladenosine biosynthesis protein Gcp
Protein pI : 4.89
Protein Weight : 35809.93
Protein Length : 458

Protein Sequence : Show Sequence

>EQA95212.1 putative tRNA threonylcarbamoyladenosine biosynthesis protein Gcp [Glaesserella parasuis 29755]
MKILGIETSCDETGVAIYDEAKGLVANQLYSQIEMHADYGGVVPELASRDHIRKTLPLIQEALKEANLTA
SDIDGVAYTAGPGLVGALLVGSTIARSLAYAWNVPALGVHHMEGHLLAPMLEENAPEFPFVALLVSGGHT
QLVDVKNVGEYELLGESIDDAAGEAFDKTAKLLGLDYPGGAALAKLAETGTPNRFTFPRPMTDRPGLDFS
FSGLKTFAANTINANLNEKGELDQQTRCDIAYAFQQAVIETLIIKCRRALQQTGYKRLVIAGGVSANKQL
RHDLSELMKQIGGEVFYPRPQFCTDNGAMIAYAGFLRLKNGEQTDLSVSVKPRWPMVELCPIDV

Molecule Role : Protective antigen
Molecule Role Annotation : (Li et al., 2015)

5. hsdS

Gene Name : hsdS
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : ACL32369
Other Database IDs : CDD:279728
CDD:223804
Taxonomy ID : 557723
Protein Name : type I restriction enzyme specificity protein HsdS
Protein pI : 7.36
Protein Weight : 45559.72
Protein Length : 509
Protein Note : Type I restriction modification DNA specificity domain; pfam01420

Protein Sequence : Show Sequence

>ACL32369.1 type I restriction enzyme specificity protein HsdS [Glaesserella parasuis SH0165]
MIESRFIEKLLKNREIEFKSLGDVTEMKRGKTITAKDASGGDIPVISGGQKPAYYHNEYNRNGKTITVAG
SGAYAGFIMYWEEPIFVSDAFSIKSDETLLDLKYVYHFLLQHQQKIYGMKKGSGVPHVYPKDLSTLVIPI
PPLDVQQEIVRILDAFTSLTAELTAELTAELTSRQKQYQYFRDKLLNFDDISDRGGYETNPITKALWHNK
KVVFKTLGEVTTISIGLTYTPAYVEKGIKFISAQNTSKDYLDLSNVKYISEEEFENSTDNAKPQRDDILF
TRVGSNIGHPVIVETDEKLCIFVSLGFLRVKDNNFLFNRYLKHWMSTDLFWKQVEKNVHGSAKINLNTGW
LKDFKIPIPPFEEQQRIVAILDKFETLTNSIAEGLPKEIELRRKQYEYYREKLLSFPQ

Molecule Role : Protective antigen
Molecule Role Annotation : (Li et al., 2015)

6. hxuB

Gene Name : hxuB
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : ACL33615
Other Database IDs : CDD:225387
CDD:285653
CDD:279448
Taxonomy ID : 557723
Protein Name : heme/hemopexin-binding protein B, hemolysin activation/secretion protein
Protein pI : 9.69
Protein Weight : 61003.04
Protein Length : 690
Protein Note : Hemolysin activation/secretion protein [Intracellular trafficking, secretion, and vesicular transport]; COG2831

Protein Sequence : Show Sequence

>ACL33615.1 heme/hemopexin-binding protein B, hemolysin activation/secretion protein [Glaesserella parasuis SH0165]
MKHFRRKNVITGIILSCVSGFTYANPIPDSGTLNKQLEQTRQIQVAKVKPSGELFKTPTQEENLPANKES
LKFRLDSIKLLELDGNEIQDNLTSITSPYLHQEITLDDLNRLSQQITNYYRDNNYLVAKAFIPPQEINNG
ELQIFVIKGSLDQVVVRNGSRLKEDLVMRMVNTTVKSHSYLVKNELEKLALLLNDIKGITSALSLKAGQN
TGKTDLTVNLKDNKRWNGYISIDNQGNDNTGKYRTTLGNKIYNLVGLGDELHFDVLASNTFDLYNLRADY
SFIIDGYGTRVGVNSNYLKYKLGANFKDLNAQGSTYNLGGYITHPTIRTASLRLDSKLAFNHQKLSDEQK
SVDLQQKRKVNTTSIGVSGSWNSIKEGVTYFSFNTIFGKESHSTNEKKHYLSENYQPKRTFTTFNYSLSH
EQFLPKSFSITFAASGQFADKNLDSGNKFLLGGLSGVKGYQAGTVSVDEGHIIQAELKHYLPVFSKSILV
SSLFYDEGFGKQYKNTEKLSESVENKVRLKSIGVGLNLIEPNHYSINFAYAKPIGKKYNNVDKNQFWLSA
IKTF

Molecule Role : Protective antigen
Molecule Role Annotation : (Wen et al., 2016)

7. hxuC

Gene Name : hxuC
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : ACL33614
Other Database IDs : CDD:273807
CDD:238657
Taxonomy ID : 557723
Protein Name : heme/hemopexin utilization protein C/outer membrane receptor protein, mostly Fe transport
Protein pI : 9.8
Protein Weight : 77928.54
Protein Length : 853
Protein Note : TonB-dependent heme/hemoglobin receptor family protein; TIGR01785

Protein Sequence : Show Sequence

>ACL33614.1 heme/hemopexin utilization protein C/outer membrane receptor protein, mostly Fe transport [Glaesserella parasuis SH0165]
MRLSKIYTALFLSLPTVSLADSNVSAELAEISVIASRDAFHWANHTSEKATLNKNQLLTQQPISTADAIK
RITNVDIAGGSRGIAQKPIIRGLSGNRVVQVIDGVRQNFDLSHRGSYFLPMALTQEIEVIKGPTSTLWGS
GALGGIVAIRTPNALDLLKDNQQIGAKIHQGYQSANSLSETDVSVFAANDKFDGLVSGFYNHADDLRLAD
SKKLVDSRYTQKGGLFKFGWQINDENRLELSHRLSHFEQIAPTNNEVAEEFTNQDVRNLIRNWHRNNPTA
SFAEMTRFYKGLTSVLGSVSYRAKQKITDQSSALHYYFNPKDNDYINSQLTLYRNSTKEKEQRIASGLQD
QTKLSTMGLNIRNSTDLNWLALNYGVDFISDRVVTQREKNATTPFRPNNYDAKSKNSAFYLLTHIPLWNE
RIIVSPSVRYDHFKTQSDEAKYSASRWSPSIAATWKATNWLDLSTRYNKAFRTPSMQERFVSGSHFGTNL
MGQSFNNTFVSNPNLRPETAKNKEIAAKVHFDNLFVHQDKFIFNAAYFQNDVKDFIHLDIFRASQRERIP
SLSQYRNVENARLTGYELEFAYQQERLALGLNYAQTKGKNRQTQEALSNIPANKLGFTIDYALVPQTFMI
GANITHYASQKRVPKTHAMTYPSYTLTDLKASYTPSGEWRNLRLDMMIENVFDKKFQPAFSLMEGAGRNI
KLNASYQF

Molecule Role : Protective antigen
Molecule Role Annotation : (Wen et al., 2016)

8. LppC

Gene Name : LppC
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : WP_021117166
Other Database IDs : CDD:332423
Taxonomy ID : 738
Protein Name : lppC lipofamily protein
Protein pI : 5.9
Protein Weight : 61497.82
Protein Length : 653
Protein Note : LppC putative lipoprotein; cl27602

Protein Sequence : Show Sequence

>WP_021117166.1 lppC lipofamily protein [Glaesserella parasuis]
MPTILVQSYGFRQKMKTIFIPTALALLLAACSGNKVTETLKNEAYGNSEFYINKAEQSRNIEEQQSYRLL
AVRKLIEENKVVEAQNTFSEILVAKLNDEQKLEHRLLIAQLAALQGNNSEAQGVLRALPQTLLSQSQRLR
VYQTQARIAENQNDVIAAVNARALMDRYMTDTQSRQANNDKIWEMLRNANRGMLEKAVAGPGEIGLAGWL
ALVVAYNQNMSNPAQLPQVIEMWKQQYPNHSATLLLPIELRNVSSFEQTQLNGVALLLPLSGDAKILGEI
IKRGFDDAKGITTMPVQVFDTDSAPINDLLMQAKQQGAQTIIGPLLKPRVDEMLISPEISNINVLALNST
PNVRAVAKVCYYGLSPEAEAHFAAERFLKDGLQNAVVVAPNGDFGTRSAEAFAQRWRQLTNRDTDIRYYN
QAFEVASLLQGSVLGQGSGLYALGTAEQLSDLKQSLDNSPLANQFPIYTSSRSNSPNNGPDFRLTMEGVK
FSEIPLLSDPSSSEYKKAEQIAESDFSMMRLYAMGSDTWSIANKFNEFRQIPGYKVHGLTGVLRAGPNCN
LEREMNWLQYRGGSIVDAN

Molecule Role : Protective antigen
Molecule Role Annotation : (Li et al., 2017)

9. ndk

Gene Name : ndk
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : KEZ22119
Taxonomy ID : 738
Protein Name : Nucleoside diphosphate kinase
Protein pI : 6.53
Protein Weight : 15381.74
Protein Length : 207

Protein Sequence : Show Sequence

>KEZ22119.1 Nucleoside diphosphate kinase [Glaesserella parasuis]
MIQQTLAIIKPDATKRHLIGEILSYMEKNGLAIKALKMLHLTKEQAEGFYAEHQGKDFFDPLVAFMISEP
IVVAVLEGENAVENYRLLMGATKPEERKLGTIRKMFGLGYRENSVHGSDSETSAKREIAYFFTPSEIV

Molecule Role : Protective antigen
Molecule Role Annotation : (Li et al., 2015)

10. omp26

Gene Name : omp26
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : ACL32860
Other Database IDs : CDD:332039
Taxonomy ID : 557723
Protein Name : outer membrane protein 26
Protein pI : 8.4
Protein Weight : 28348.36
Protein Length : 341
Protein Note : Outer membrane protein (OmpH-like); cl27218

Protein Sequence : Show Sequence

>ACL32860.1 outer membrane protein 26 [Glaesserella parasuis SH0165]
MKNLFKLAAVASALAFSANTVNANEKIGFVDPNFLIQNHPLTIEANDKFTKFMKDTESKFAPREKQLAAE
NKALSDEEKALLDERKKIEEDAQKLQKEQVTLEAAMKKKVAQLEKDAPRLKAKEIQARQDKINAEAKPFQ
NKVSALQQREVEFGKKAEEFQKRADEFQKKVAAFQEEIAKVQKESGVITPEQVQQKVVEDINAKIKQVAE
SKGYTLVLPPSVALYAKDGNAADITEEILVAMGGKMPEAPKTEVPAAEVTKPEEVKK

Molecule Role : Protective antigen
Molecule Role Annotation : (Li et al., 2016)

11. oppA

Gene Name : oppA
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : ACL32119
Other Database IDs : CDD:173869
CDD:185059
Taxonomy ID : 557723
Protein Name : putative oligopeptide transporter, periplasmic-binding protein, OppA
Protein pI : 6.99
Protein Weight : 55956.24
Protein Length : 634
Protein Note : The substrate-binding component of an ABC-type oligopetide import system contains the type 2 periplasmic binding fold; cd08504

Protein Sequence : Show Sequence

>ACL32119.1 putative oligopeptide transporter, periplasmic-binding protein, OppA [Glaesserella parasuis SH0165]
MASIQRIVQFIKKGLFVTACIVGFVACDPEENNGSVRPLPNAEPTNVLSRAIYATELQLDPHFVRISAEA
GVVRDLFLGLMAFDPKGQIQNAVAKEWFSEDGKNWLFILDENAKWSNDQAVTSADFVASWQRLSDPKNAS
PLAHYLVYMGIVNSRAVLAGELPPDALGVKALNPYTLQVQLEKANYQLPKMLAHSALLPTYLGQKPNSTD
LVSNGSYRVEHFEKNLLQLKARDKNTPFQVVQYHLITSVQNPSRFDIIENPLENYHQHEKRFARLCNYFY
EFNFNDPLFAQKPIRQAVRAMISPIEISKGNGIPSHLMIPKQLFSEMGHSLSHTNVEQLLNEQGIHSGNP
LKLTLSYDRQGQHSQIAARIVRALGQSELFRVQLQAVSWEELLAKREHKDFQLIRSGWCADYPDPVLFLT
QFHSQSPDNKSSYSNAKVDELLLQLQEQPLDTAQRQRIIATIAHQLEEDVAILPLFQYQRRMSIAPTIQG
FDEQNASETIYSKDLTRQYHKDK

Molecule Role : Protective antigen
Molecule Role Annotation : (Macedo et al., 2016)

12. outer membrane protein A

Gene Name : outer membrane protein A
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : ACN62162
Other Database IDs : CDD:236764
CDD:279698
CDD:143586
Taxonomy ID : 738
Protein Name : outer membrane protein A
Protein pI : 9.52
Protein Weight : 36308.42
Protein Length : 435
Protein Note : outer membrane protein A; Reviewed

Protein Sequence : Show Sequence

>ACN62162.1 outer membrane protein A [Glaesserella parasuis]
MKKSLIALAVSGLAVASVAQAAPQANTFYVGAKAGWATFHNDINQIDSKYANDARYDATNLKYGISRNSV
TYGVFGGYQIIDNLAVELGYDYFGRVRGNKQEFRAFKHSAHGTHLSLKPSYEVLNGLDVYGKVGAALVRN
DYKRYSQTAGVQTQKAHNLKTSLVLGAGVEYAILPELAFRVEYQWLSNVGNFTKAEAKENRRATYNYSPD
SHSVTAGISYRFGQGAAPVAAAPEVVTKNFAFSSDVLFDFGKANLKPAAAQTLDAVHTEIVNLGLANPAV
QVNGYTDRIGKDAANLTLSQKRAETVANYIVSKGVNPANVTAVGYGEANPVTGNTCDAVKGRKALITCLA
PDRRVEIQVQGSKEVSM

Molecule Role : Protective antigen
Molecule Role Annotation : (Tian et al., 2011)

13. outer membrane protein D15

Gene Name : outer membrane protein D15
Sequence Strain (Species/Organism) : Haemophilus parasuis str. Nagasaki]
NCBI Protein GI : ACI05267
Other Database IDs : CDD:236834
CDD:284624
CDD:279448
Taxonomy ID : 738
Protein Name : outer membrane protein D15
Protein pI : 7.65
Protein Weight : 85698.04
Protein Length : 881
Protein Note : outer membrane protein assembly factor YaeT; Provisional

Protein Sequence : Show Sequence

>ACI05267.1 outer membrane protein D15 [Glaesserella parasuis]
MKKLLLSTLLVANGVAFASPFVIKDIRIDGIQTSVSQTMIPTLPVKVGQLATDNDIANVIRHLFVQNRFQ
NISATREGDILIIKVVENPVINNVDIEGNSAIPKEPLEQNLKANLITKGELFNSTKLDAFKSALIEHYHS
IGRYNATIDTVITNSDNGGVNIKLDIKEGDVAYVKAINFEGNQAFTSKVLMKQLDIQPDVSWFNIFQSSK
FEQTAYNQDLENLRNFYMNQGYAKFNVEAEQPVFSDDKKEVSLTYKINEGIPYTVSEIRIVGNTAKLDNE
LNEQLKYYKTGELFSKNELSKIEDNIKQVFGNNGFASAKIELHPTFDDTNKTIRLTFVVDAGQRVYVRQI
RFEGNDVTADSTLRREMRQQEGAWLSTSLAALGKARLERTGFYDSVEMSYPNVPNTADQVDIVYKIKERN
TGSINFGVGYGTESGLSYQAGIKQDNFLGMGSTISLNGTRNDYGTSINLGYTEPYFTKDGVSLGGNIFYE
DYDNSKNNSVASYKRQTYGVNSTLGFPVNEYNSYFLGLGYTQDLIKNARREFNRELYVQSMKFPIDNDLN
VYKKIKANDFDFSFGWNYNSLNRGFLPTEGTLTSISGRITTPGSDNKYYKVSIDFKNYLPVNREHKWVIS
TKANIAYANGFSGKELPFYQTYTAGGIGSLRGFAYGAIGPKAIYLGKDNNGNIRFNSVNRDIVGGNAMAS
ATLELITPTPFVSEKYQHNVRTSLFVDAATVLNTKWKTNPVVNDIQYNYSSLENFKDYKRFRASTGIAFQ
WNSPIGPLVFSYAKPIKKYQGDEIEQFQFSIGGSF

Molecule Role : Protective antigen
Molecule Role Annotation : (Zhou et al., 2009)

14. outer membrane protein P2

Gene Name : outer membrane protein P2
Sequence Strain (Species/Organism) : Haemophilus parasuis str. Nagasaki]
NCBI Protein GI : EYE71716
Taxonomy ID : 1117322
Protein Name : outer membrane protein P2 (OMP P2)
Protein pI : 9.55
Protein Weight : 36557.65
Protein Length : 451
Protein Note : COG3203 Outer membrane protein (porin)

Protein Sequence : Show Sequence

>EYE71716.1 outer membrane protein P2 (OMP P2) [Glaesserella parasuis str. Nagasaki]
MKKTLVALAVAAFAASASAVTVYENEGTKVDFDGQLRLLLEEQATKEKGQSSTGGHTNLKNNGSRFGISI
KHNINENLYGFGRYETRLDSNSKNAAGWGDVKTKYAYVGLGGYGHEISFGKQDVIGDSIGQAGFDKVYGV
GTGGIKYSANNTNKKGFDILTSDSDSAINYTYTGIEGLTLGANYNVANERDKKTGEVNVGSTKSGFGLGA
KYTAKIAESQSVTVAAGYTHDDYKSGSVNKKDKDGVYFGLKYVNAPFTVAVDGGHGVEKTGNVKEKIDFV
RTGARFDVTPKSGVYGNYSYGTYKDKAYKATAHQFMLGADYKLHKQVVTFVEGRLIKNKDSNNKKVTDQA
LGVGLRVLW

Molecule Role : Protective antigen
Molecule Role Annotation : (Zhou et al., 2009)

15. palA

Gene Name : palA
Sequence Strain (Species/Organism) : Haemophilus parasuis SH0165
NCBI Protein GI : ACL31779
Other Database IDs : CDD:274307
CDD:143586
Taxonomy ID : 557723
Protein Name : 18K peptidoglycan-associated outer membrane lipoprotein, precursor PalA
Protein pI : 7.56
Protein Weight : 16062.54
Protein Length : 272
Protein Note : peptidoglycan-associated lipoprotein; TIGR02802

Protein Sequence : Show Sequence

>ACL31779.1 18K peptidoglycan-associated outer membrane lipoprotein, precursor PalA [Glaesserella parasuis SH0165]
MKKLAKVLMIAAPAFVLAACSSSNDNASANASANAGQTFGGMSVQDLQTRYNTVYFDFDSYTLKAEDQQL
LDAHAQYLVASKGKVTVAGHADERGTPEYNIALGQRRADAVKDFLATKGASNVATVSYGEEKPAVLGHSE
ADYSKNRRAVLEY

Molecule Role : Protective antigen
Molecule Role Annotation : (Zhou et al., 2009)

16. pflA

Gene Name : pflA
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : AMW15937
Other Database IDs : CDD:182994
CDD:302752
CDD:100105
Taxonomy ID : 738
Protein Name : pyruvate formate lyase-activating enzyme 1
Protein pI : 5.79
Protein Weight : 26955.76
Protein Length : 330
Protein Note : pyruvate formate lyase-activating enzyme 1; Provisional; PRK11145

Protein Sequence : Show Sequence

>AMW15937.1 pyruvate formate lyase-activating enzyme 1 [Glaesserella parasuis]
MTVLARYHSYESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWDLEGGKEISVENLMKEVVTYKHFMRAT
GGGVTASGGEAVLQMEFVRDWFRACKAEGIDTCLDTNGFVRNYSELVDEMLDVTDLVMLDLKQLNEEIHK
DFIGVSNKRTLDFARYLHKRNQRTWIRYVVVPGYTDADEDIHLLGQFIQGMDNIEKVELLPYHRLGAHKW
KTLGLEYELEDVMPPTKESLEHIKGIIESYGHTVKY

Molecule Role : Protective antigen
Molecule Role Annotation : (Li et al., 2015)

17. plpA

Gene Name : plpA
Sequence Strain (Species/Organism) : Haemophilus parasuis SH0165
NCBI Protein GI : ACL32115
Other Database IDs : CDD:304360
CDD:270316
Taxonomy ID : 557723
Protein Name : ABC-type metal ion transport system, periplasmic component/outer membrane lipoprotein 1 precursor
Protein pI : 6.05
Protein Weight : 27893.2
Protein Length : 425
Protein Note : Type 2 periplasmic binding fold superfamily; cl21456

Protein Sequence : Show Sequence

>ACL32115.1 ABC-type metal ion transport system, periplasmic component/outer membrane lipoprotein 1 precursor [Glaesserella parasuis SH0165]
MNFKKLLGLAVVSALALTGCKEEKKAEAPAVAPAPAAQAQKIKVGVMSGPEHTVAEKAAQIAKEKYGLEV
EFVLFNDYALPNTAVSKGDLDANAFQHKPYLDKDSASKGLDNLVIVGNTFVYPLAGYSKKIKNVAELQDG
AVIAVPNDPSNLARALILLEKQGLIKLKDNTNLFSTSLDIVENPKNLKIQEVDTSVAAKALDDVDLAVVN
NTYAGQVGLNATEHGVFVENKESPYVNIIVARKDNQASEAVQNFMKAYQTEEVFQEAQKHFKDGVVKGW

Molecule Role : Protective antigen
Molecule Role Annotation : (Hong et al., 2011)

18. rnfC

Gene Name : rnfC
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : KEZ22306
Taxonomy ID : 738
Protein Name : Nitrogen fixation protein rnfC
Protein pI : 8.11
Protein Weight : 75511.08
Protein Length : 819

Protein Sequence : Show Sequence

>KEZ22306.1 Nitrogen fixation protein rnfC [Glaesserella parasuis]
MQADVLSRIREGKLWDFPGGIHPPEMKKQSNQKPIRSLHLPKFFYVPVLQHSGTAGDVIVQQGDLVLKGQ
ALTKGDNYRQLPVHSPTSGKVVAIQPHVSSHPSGLPELTIVIETDGEDKWIERQPIDDFLSLTSDQIIQK
VYQAGIAGLGGAVFPTSHKLHFADKRCKLLIINGAECEPYITCDDRLMQDYADEMIEGVRILRYVLRPED
VVIAIEDNKPKAIQAVEKALKGANDISVRVIPTKYPSGVSDQLVQVLTGLEIPQGKRTIEMGIVMHNVGT
AFAVKRAVMDDEPLIERVVTLTGDKIRNKGNVWARLGTPILHLLEQVDYQADERFPVFLGGPMMGFILPS
LQSPITKTANCIIAPDHFEYAPPEPERNCIRCSSCSDACPVGLLPQQLYWFARAEDHDKSKEYHLDACIE
CGVCAYVCPSYIPLIQYFRQEKAKIQEVDQKAKQAEEAKIRFEAREARLNKEKEARTARIQQAAEKRREE
VANSQGEDPVKAALERLKAKKAETATPVQTKANGEPDNSELMAQRKARRLAKQVEVSPLPDSRDITSLES
VPLPQAGEGNDPRKAAVAAALARAKAKKEAQQAVTSDENTAKAEVAEDPRKAAVAAALARAKAKKEAQQA
AVSDENVAKAEVAEDPRKAAVAAALARAKAKKEAQQAVVSDENVAKAEVAEDPRKAAVAAALARAKAKKE
AQQAVVSDENVAKAEVAEDPRKAAVAAAIARAKAKKEAQK

Molecule Role : Protective antigen
Molecule Role Annotation : (Li et al., 2015)

19. superoxide dismutase

Gene Name : superoxide dismutase
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : AIK91190
Other Database IDs : CDD:224943
CDD:238186
Taxonomy ID : 738
Protein Name : superoxide dismutase
Protein pI : 7.82
Protein Weight : 18281.37
Protein Length : 241
Protein Note : Cu/Zn superoxide dismutase [Inorganic ion transport and metabolism]; COG2032

Protein Sequence : Show Sequence

>AIK91190.1 superoxide dismutase [Glaesserella parasuis]
MKKTVLTLALTALFGFSTSAIANSATQIEVKVQQLDLQNGNKDVGTVTITESPYGLVFTPNLKGLSHGLH
GFHIHEKPSCEPKEKDGKLVAGLGAGGHWDPKETKKHGYPWSDEAHLGDLPALAVDHEGNATNPVLAPRL
KKLEEVKGRSLMIHAGGDNHSDHPAPLGGGGARMACGVIN

Molecule Role : Protective antigen
Molecule Role Annotation : (Guo et al., 2017)

20. tbpA

Gene Name : tbpA
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : AAV68905
Other Database IDs : CDD:273799
CDD:238657
Taxonomy ID : 1117322
Protein Name : TbpA
Protein pI : 9.54
Protein Weight : 97840.98
Protein Length : 967
Protein Note : TonB-dependent lactoferrin and transferrin receptors; TIGR01776

Protein Sequence : Show Sequence

>AAV68905.1 TbpA [Glaesserella parasuis str. Nagasaki]
MKNKLNLISLAILSLFAVQSYAEQAVQLNDVYVTGTKKKAHKKENEVTGLGKVVKTPDSLSKEQVLGMRD
LTRYDPGISVVEQGRGATTGYSIRGVDRNRVGLALDGLPQIQSYVSQYSRSSSGAINEIEYENLRSIQIS
KGASSSEFGSGSLGGSVQFRTKEVSDIIKPGQSWGLDTKSAYSSKNQQWLNSLAFAGTHNGFDALVIYTH
RDGKETKAHKDAESRSKSIQRVDLADNNPQGSNWFKVKNDCPSLNCEPRQQVGVSYDGQKYITEQLSAKE
YTGEERALPDPVKYKSDSWLVKLGYSLSPKHYVAGVYEHSKQRYDTRDMTYPAYWQLSDLKNGDKWYPMN
NAKGLYRDNALDGVAIDYFTEDGVKSSKGLRWAKARFIDEWHTRDRLGALYRYTDQDGNRLIDRLSLSFD
QQKINLSTRLRENNCSEYPTIDKNCRATLDKLWSSTKNEQSSYEEKHDTIQLSLDKTVQTGLGKHQLNML
LGSDRFNSTLKRHEILSEFSVGTWHRIRGNGYKDTPYIYELKDQAIYSKNECDYSGTIAGRADCATSKIK
GHNHYIALRDNFAITKYLDIGLGYRFDKHEFRSTHRWANQGDYKNSAWNIGIVAKPTSFLSLSYRASSGF
RVPSFQELFGLRYDGAMKGSSDAYQKTEKLSPEKSLNQEVATTFKGDFGVVEVSYFKNDYKQLIAPAERM
HQTQSMINYFNVQDIKLDGINLIGKLDWNGVFDKIPEGIYTTLAYSKMRVKEVKNYQGYMNIRSPLLDTI
QPARYVVGVGYDQPDEKWGVNLTMTHSSGKNPNELRGNEQVGFANYERTATKKRTRSWHTFDLTGYITPW
KHTTVRAGVYNLMNYRYTTWESVRQSSLNAIHQHTNVKDYARYAAPGRNYVVSFEMKF

Molecule Role : Protective antigen
Molecule Role Annotation : (Huang et al., 2013)

21. tbpB

Gene Name : tbpB
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : AAV68899
Other Database IDs : CDD:279621
Taxonomy ID : 738
Protein Name : TbpB
Protein pI : 6.92
Protein Weight : 62210.98
Protein Length : 643
Protein Note : Transferrin binding protein-like solute binding protein; pfam01298

Protein Sequence : Show Sequence

>AAV68899.1 TbpB [Glaesserella parasuis]
MHFKLNPYALAFTSLFLVACSGGKGGFDLEDVRPNQTAKAEKATTSYQDEETKKKTKEELDKLMEPTLGY
ETQILRRNKAPKTETGEKRNERVVELSEDKITKLYQESVEIIPHLDELNGKTTSNDVYHSHDSKRLDKNR
DLKYVRSGYVYDGSFNEIRRNDSGFHVFKQGIDGYVYYLGVTPSKSYQRESHSYKGTWDLLSNINLDREI
DGFDTSGDGKNVSATSITETVNRDHKVGEKLGDNEVKGVAHSSEFAVDFDNKKLTGSLYRNGYINRNKAQ
EVTKRYSIEADIAGNRFRGKAKAEKAGDPIFTDSNYLEGGFYGPKAEEMAGKFFTNNKSLFAVFAAKSEN
GETTTERIIDATKIDLTQFNAKELNNLGDASVLIIDGQKIDLAGVNFKNSKTVEINGKTMVAVACCSNLE
YMKFGQLWQKEGKQQVKDNSLFLQGERTATDKMPAGGNYKYVGTWDALVSKGTNWIAEADNNRESGYRTE
FDVNFSDKKVNGKLFDKGGVNPVFTVDATINGNGFIGSAKTSDSGFALDAGSSQHGNAVFSDIKVNGGFY
GPTAGELGGQFHHKSDNGSVGAVFGAKRQIEK

Molecule Role : Protective antigen
Molecule Role Annotation : (Barasuol et al., 2017)

22. TolC

Gene Name : TolC
Sequence Strain (Species/Organism) : Haemophilus parasuis
NCBI Protein GI : WP_075604781
Other Database IDs : CDD:224455
Taxonomy ID : 738
Protein Name : TolC family protein
Protein pI : 9.06
Protein Weight : 49474.35
Protein Length : 530
Protein Note : Outer membrane protein TolC [Cell wall/membrane/envelope biogenesis]; COG1538

Protein Sequence : Show Sequence

>WP_075604781.1 TolC family protein [Glaesserella parasuis]
MKVSKLTFTLLSALVLSACTNMSNDGSLQKAQQDYQQYQDITKQYQINEQWWLGYNDTQLNRLVETALGN
NINLAKTAISVNKALYNANLVGANLVPTFTASEKSSASKGVGSSSNLNSTGTSSIGHQIGLNLSYTLDLW
GRLRDTASAAEWEHKATQEDLQAARLSLINAVVSSYYNLAYYQDAIRITQQSVKSYEQLNHILSNKFKQG
LIDQLSVDQSMQAVLSAQNALINLQSAQKSSEQVLRNLLNLKPNEPLVVNYPSILKVKLQDVDVNVPVST
IANRPDVLASLKRLQGAFKNLTATENSWFPTVTLGGSLTGSSTKFNNTSDNPVAGGVVSFDLPFLDWNRV
QNNIKISEEGYKLAKLNYEQTVTTALNEIDTYYSTYQLSKSGYANLQKKYEYDRKISGYYKNRYEQGISE
LREWLSAMNTERSSELSLLENKYTLLKNENAIYQAMAGKYRK

Molecule Role : Protective antigen
Molecule Role Annotation : (Li et al., 2017)

23. VtaA5

Gene Name : VtaA5
Sequence Strain (Species/Organism) : Haemophilus parasuis str. Nagasaki
NCBI Protein GI : EYE71559
Taxonomy ID : 1117322
Protein Name : VtaA5
Protein pI : 9.78
Protein Weight : 102402.53
Protein Length : 1172
Protein Note : COG5295 Autotransporter adhesin

Protein Sequence : Show Sequence

>EYE71559.1 VtaA5 [Glaesserella parasuis str. Nagasaki]
MNKIFRVIWSHAQQAWVVVSELVKSHTKTSAYTDKRAQVCTSDYFLDKQQDKFKLSLLSLVLLSIFFSPV
AVGAQLHTGTPYTAGGGSKSDRGSDDGTISIGKKSKASYGAIAIGEESKAEARHNVAIGYKADSGTDANS
ITIGYNTKVSGEQGIAIGKQSKAGGRSVVLGGGAEGTTTQTVVIGNLAKATGSQSIAIGANTKAEGYGSI
SIGGDDLDSTAYHDNNSTKQTRQTTTAKGKASVAIGGLSLATGDGSTVLGPLASASHVEGIAIGARSKST
NEYGIAVGGSATAGKNAVAVGRDSKGAGTDSIAIGNSAKTTGADSVVVGASINVTGEKLVAIGRQANAGS
HSTALGYKASAGGMHSVAVGESAMTNDGAARATALGNNTVVTVGGGVALGYGSRAETRGGIEGAKQTYSV
TTGGSTVENGFKSTENVDGNNIGAVSVGGGSGNKLINRQITNVAAGKELTDAVNVAQLKSLTMKIAGDTG
NSDNEKVGIWEGTLKVLGTSGEIKTSASNDTITLKLDETLKNKIDKIDNLGWKLKIAQGMGGKATNPPAE
QLIKMDQTVTFTAGKNIKLEQAGGNITISTIGKLIEKTEWKNGNLQITYTDGSSDTIAKGKDGKNGEKGD
RGEPGPRGEPGIPGPAGPVGPMGPAGAAGLVGPMGPQGPAGPPGPVGPAGAAGPKGDKGEPGQAGPAGPR
GPAGPKGDTGQRGETGSAGPAGPKGEPGPKGEQGIPGQAGPAGPRGPQGTAGAQGPKGDKGEPGEAGPAG
PRGPAGPKGDAGPKGDTGQRGETGSAGPTGPKGEPGPKGEQGIPGPAGPAGPKGDKGDTGPAGPQGPAGP
TGPQDPAGPTGNSELKGITSIANGNDATKANGAKITLSAGSTDKTVNVNDAKITNVAAGTADTDAVNVSQ
LNTKAAASKTEVEAGKNVKVTSKTGANGQNIYNVSVSGDLSDITSISNGDTKVSLGKDKQGNPVVNMNGA
RITNVGDGSAEGDIVNVRQLNKVVSSVNTGFNQLSRDIGRVDVNARAGIASAGAMANLPQISLPGKSAIS
VSNAQYRGQSAYAIGYSRISDNGKWLIRASVSSNTQRDTMIGGGVGFVW

Molecule Role : Protective antigen
Molecule Role Annotation : (Olvera et al., 2011)

24. VtaA6

Gene Name : VtaA6
Sequence Strain (Species/Organism) : Haemophilus parasuis str. Nagasaki
NCBI Protein GI : EYE72607
Taxonomy ID : 1117322
Protein Name : VtaA6
Protein pI : 9.78
Protein Weight : 96152.6
Protein Length : 1125
Protein Note : COG5295 Autotransporter adhesin

Protein Sequence : Show Sequence

>EYE72607.1 VtaA6, partial [Glaesserella parasuis str. Nagasaki]
MNKIFRVIWSHDQQAWVVVSELVKSHTKTSACTDKRAQVCTSHYFLDKQQDKFKLSLLSLVLLGIFFSPP
AGSATHSGTPYTADGGSRSNLGSDDGTISIGKESKASYGAIAIGQRSKAEGRHGIAIGYQTNAGTHVNSV
AVGNDIRVSGEEAVAIGSSSKAGKGSVVLGRQADAANIEQAVVIGNLAKASKAQSIAIGANTKAEGYGSI
SIGGDDLKTTKYQTGNQGQSKTTTAKGKASVAIGGLSLATGDGSIVVGPLASASHVEGIAIGARSKSTNE
YGIAVGGGATAGKNAVAVGRDSNGGGTDSIAIGNSAKTTGADSVVVGANINVTGEKLVAIGREANSGSHS
TALGYKASAGGMHSVAVGESAMTNDGAARATALGNNTVVTVGGGVALGYGSNASTAGGVEGLKQTHSVTT
EGSTVANGFKSTQSVDNNAIGAVSVGGGSGNKLINRQITNVAAGKELTDAVNVAQLKSLTLKIEGNENKH
NGVNANLSSADRPKVGLWSGTLKVKGDNGLTSHASGDTITVKLTDDTKSKIDKIDNLGWKLKIAQGMGGK
ATNPPTEHLVKMSDTATVTFTAGNNIKLEQTNGNITISTIGKLIKETKMVNGNLQITYTDGSSNTITRGE
KGDTGPRGETGPAGPPGPMGPAGAAGPAGAAGLVGPMGPQGPAGPPGPVGPAGAAGPKGDKGEPGQAGPA
GPRGEPGQAGPAGPRGPAGPKGDAGPKGDTGQRGETGPAGPAGPKGEPGPKGEQGIPGPAGPTGPKGDKG
DTGPAGPQGPAGPTGPQGPAGPAGAKGDKGDTGPAGPQGPTGPTGSQDPAGPTGNSELKGITSIANGNDA
TKANGAKITLSADSTDKTVNVNDAKITNVAAGTADTDAVNVSQLNTKAAASKTEVEAGKNVKVTSKTGAN
GQNIYNVSVSGDLSDITSISNGDTKVSLGKDKQGNPVVNMNGARITNVGDGSAEGDIVNVRQLNKVVSSV
NTGFNQLSRDIGRVDVNARAGIASAGAMANLPQISLPGKSAISVSNAQYRGQSAYAIGYSRISD

Molecule Role : Protective antigen
Molecule Role Annotation : (Olvera et al., 2011)

25. VtaA8

Gene Name : VtaA8
Sequence Strain (Species/Organism) : Haemophilus parasuis str. Nagasaki
NCBI Protein GI : EYE72886
Taxonomy ID : 1117322
Protein Name : VtaA8
Protein pI : 9.41
Protein Weight : 94207.97
Protein Length : 1080
Protein Note : COG5295 Autotransporter adhesin

Protein Sequence : Show Sequence

>EYE72886.1 VtaA8 [Glaesserella parasuis str. Nagasaki]
MNKIFRVIWSHAQQAWVVVSELVKSHTKTSAYTDKRAQVCTSDYFLDKQQDKFKLSFLSLVLLGIFFSPV
GSARLFLDEARQGSAGVDDGSIGIGRESKVGPGSITIGQKSEAEGRTAVAIGYSAKTGSEHDNAIAIGNS
SKATGPGTVTVGHSSQAKDRSVVLGANAEGKNAQTVVIGTLAKASASQSIAIGADTKAEGYGSISIGGDD
LDQTKYNDNNSTNQPRHTTTAKGKASVAIGGLSLAEGDGSIVVGPLASASDVEGIAIGARSKSTNEYGIA
VGGGATAGKNAVALGRESKGAGTDSIAIGNSARTTGADSVVVGANIDVTDEKLVAIGYQASAKSHATALG
YMASAGGMHSVAVGESAMTNDGAARATALGNNTVVTVGGGVALGYGSRAETRGGIEGAKQSYSVTTGTSS
VDNGFKSTGSVDNNSIPIGAVSVGNNKIKRQIVNVAAGKELTDAVNVAQLKSLTLKIAGNTSEQTQPAVG
LWEGTLKVMGTSGEIKTSASGDTITLKLDETLKNKINTIDNLGWNLKIAKGAGEANPPNAAHLIKMSDTA
TVTFTAGNNIKLEQTNGNITISTIGKLIAKTEWENDGLKITYTDGMHDIIKKGEKGDRGERGLRGETGPA
GPAGPMGPAGAAGLVGPMGPQGPAGPPGPVGPAGAAGPKGDKGEPGQAGPTGPRGPAGPKGDAGPKGDTG
QRGETGPAGPAGPQGPAGPTGPRGDKGDTGPAGPQGPAGPTGPQGPAGPTGSQDPAGPTGNSELKGITSI
ANGNDATKANGAKITLSAGSTDKTVNVNDAKITNVAAGTADTDAVNVSQLNTKAAAARTEVEAGKNVKVT
SKTGANGQNIYNVSVSGDLSDITSISNGDTKVSLGKDKQGNPVVNMNGARITNVGDGSAEGDIVNVRQLN
KVVSSVNTGFNQLSRDIGRVDVNARAGIASAGAMANLPQISLPGKSAISVSNAQYRGQSAYAIGYSRISD
NGKWLIRASVSSNTQRDTMIGGGVGFVW

Molecule Role : Protective antigen
Molecule Role Annotation : (Olvera et al., 2011)

26. VtaA9

Gene Name : VtaA9
Sequence Strain (Species/Organism) : Haemophilus parasuis str. Nagasaki
NCBI Protein GI : EYE71168
Taxonomy ID : 1117322
Protein Name : VtaA9
Protein pI : 9.71
Protein Weight : 88376.07
Protein Length : 1024
Protein Note : COG5295 Autotransporter adhesin

Protein Sequence : Show Sequence

>EYE71168.1 VtaA9 [Glaesserella parasuis str. Nagasaki]
MNKIFRVIWSHAQQAWVVVSELVKSHTKTSAYTDKRAQVCTSDYFLDKQQDKFKLSLLSLVLLSIFFSPV
AVGAQLHTGTAFLLDGSNKSKLGTDDGTIGIGKDSKAGYGAIAIGQYSKAKARHNVAIGYKADSGVQPNT
ITIGYNTKVSGQEAIAIGKESKAGKESVVLGGQASAANIEQAVVIGHSATASASQSIAIGARSKSTADYG
IAVGGGATAGKNAVAVGRDSKGAGTDSIAIGNSAKTTGVDSVVVGANINVTDGQLVAIGREAKAGSHSTA
LGYKASAGGMHSVAVGESAMTNDGAARATALGNNTVVTVGGGVALGYGSNASTAGGVVGLKQNHSVTTGE
STVDNGFKSTESVDNNPNPIPIGAVSVGNNNIKRQIVNVAAGKELTDAVNVAQLQSLTMKISGDNSSSGK
VGIWDGTLKVVGTSGQIKTSANGDTITLKLDETLKNKIDNIDNLGWKLAITKGSGGEVTTPNTPYLIKMS
DMATVTFTAGNNIKLEQADGNITISTIGKLIAKTEWENDGLKITYTDGMHDIIKKGEKGDRGEKGPKGDR
GETGSAGPAGPAGAQGPVGPAGPAGPTGPQGATGPAGPKGEAGAAGPKGEKGDPGPKGEAGSTGPTGPAG
PKGDPGQAGPKGDTGQKGETGPAGPAGPQGPAGPAGAKGDKGDTGPAGPRGPAGPTGPQGPAGPAGPQDP
AGPTGNSELKGITSIANGNDATKANGAKITLSAGSTDKTVNVNDAKITNVAAGTADTDAVNVSQLNTKAA
ASKTEVEAGKNVKVTSKTGANGQNIYNVSVSGDLSDITSISNGDTKVSLGKDKQGNPVVNMNGARITNVG
DGSAEGDIVNVRQLNKVVSSVNTGFNQLSRDIGRVDVNARAGIASAGAMANLPQISLPGKSAISVSNAQY
RGQSAYAIGYSRISDNGKWLIRASVSSNTQRDTMIGGGVGFVW

Molecule Role : Protective antigen
Molecule Role Annotation : (Olvera et al., 2011)

27. yfeA

Gene Name : yfeA
Sequence Strain (Species/Organism) : Haemophilus parasuis SH0165
NCBI Protein GI : ACL32714
Other Database IDs : CDD:238557
Taxonomy ID : 557723
Protein Name : chelated iron ABC transporter, periplasmic-binding protein
Protein pI : 8.15
Protein Weight : 30617.57
Protein Length : 400
Protein Note : Metal binding protein PsaA. These proteins have been shown to function as initial receptors in ABC transport of Mn2+ and as surface adhesins in some eubacterial species. They belong to the TroA superfamily of periplasmic metal binding proteins that...; cd01137

Protein Sequence : Show Sequence

>ACL32714.1 chelated iron ABC transporter, periplasmic-binding protein [Glaesserella parasuis SH0165]
MKKLAALLSMLFLVSNPLAAQQFKVVTTFTVIQDIAQNVAGDKAVVESITKPGAEIHDYQPTPQDIVKAQ
SADLVLWNGMNLESWFERFFAQVKDKPAVVVTEGIEPISIYEGPYKDKPNPHAWMSTKNALIYIENIRQA
LVKYDPANAEAYNANAKAYADKIVALDQPLRERLAKVPEAQRWLVTSEGAFSYLARDYGFKELYLWAINQ
DEQGTPKQIRKVIDQVRKHQIPVVFSESTISDKPAKQVAKESKARYGGVLYVDSLSTKDGKVPTYIDLLN
VTVSTIVAGFEK

Molecule Role : Protective antigen
Molecule Role Annotation : (Hong et al., 2011)

28. ygiW

Gene Name : ygiW
Sequence Strain (Species/Organism) : Haemophilus parasuis D75
NCBI Protein GI : EQA00462
Taxonomy ID : 1117322
Protein Name : ygiW domain protein
Protein pI : 5.7
Protein Weight : 14278.97
Protein Length : 205

Protein Sequence : Show Sequence

>EQA00462.1 ygiW domain protein [Glaesserella parasuis str. Nagasaki]
MKKAIILTTLLAVSTVSMAKGDDDYRHGGYHDGKARTHQSQGFFDESIAVKSVADALKAADDTPALLEGQ
IVKQIDKDEFIFKDATGEIEIDVSKRAWNGLNIGPQDTIQIRGKVDNDWNKVQVDVKQITKK

Molecule Role : Protective antigen
Molecule Role Annotation : (Yuan et al., 2012)

III. Vaccine Information

1. Haemophilus Parasuis Avirulent Live Culture Vaccine (USDA: 19J1.00)

a. Manufacturer:

Newport Laboratories, Inc.

b. Vaccine Ontology ID:

VO_0001794

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Pig

g. Gene Engineering of gapA

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

2. pCgap DNA vaccine encoding glyceraldehyde-3-phosphate dehydrogenase

a. Vaccine Ontology ID:

VO_0004515

b. Type:

DNA vaccine

c. Status:

Research

d. Gene Engineering of gapA

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

e. Vector:

pcDNA3.1 Directional TOPO vector (pcDNA3.1D/V5-His-TOPO) (Fu et al., 2012)

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Vaccination Protocol: Four to five week old female BALB/c mice were divided randomly into three groups (30 mice per group): one group was injected intramuscularly in the hind thigh muscle with 100 μg of plasmid dissolved in 100 μl PBS; the other two groups were injected with pcDNA 3.1 vector only as negative control or PBS as blank control. Mice received a vaccination boost of an equivalent dose on day 14 and 28 following the first immunization (Fu et al., 2012).
Vaccine Immune Response Type: VO_0000286
Immune Response: Although IgG analysis showed production of both IgG1 and IgG2, the higher levels of IgG1 indicated the dominance of Th2 response over Th1 response (Fu et al., 2012).
Challenge Protocol: Two weeks after the second vaccination boost, mice immunized with pCgap were divided into groups 1 and 2 (12 mice/group). The mice from group 1 were challenged intraperitoneally with a lethal dose of 3.0 × 10^9 CFU of H. parasuis SH0165 strain and the mice from group 2 were challenged intraperitoneally with a lethal dose of 3.0 × 10^9 CFU of H. parasuis MD0322 strain. Mice were monitored for seven days post-challenge, and morbidity and mortality were recorded (Fu et al., 2012).
Efficacy: The groups vaccinated with the pCgap vaccine exhibited 83.3% and 50% protective efficacy against the H. parasuis MD0322 serovar 4 or SH0165 serovar 5 challenges, respectively (Fu et al., 2012).

3. Porcine Reproductive & Respiratory Syndrome Respiratory Form, Modified Live Virus Vaccine-Erysipelothrix Rhusiopathiae-Haemophilus Parasuis Bacterin (USDA: 49V9.20)

a. Manufacturer:

Boehringer Ingelheim Vetmedica, Inc.

b. Vaccine Ontology ID:

VO_0004216

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Pig

4. Porcine Reproductive & Respiratory Syndrome Respiratory Form, Modified Live Virus Vaccine-Haemophilus Parasuis Bacterin (USDA: 49Q9.20)

a. Manufacturer:

Boehringer Ingelheim Vetmedica, Inc.

b. Vaccine Ontology ID:

VO_0004218

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Pig

IV. References

1. Barasuol et al., 2017: Barasuol BM, Guizzo JA, Fegan JE, Mart�nez-Mart�nez S, Rodr�guez-Ferri EF, Guti�rrez-Mart�n CB, Kreutz LC, Schryvers AB, Frandoloso R. New insights about functional and cross-reactive properties of antibodies generated against recombinant TbpBs of Haemophilus parasuis. Scientific reports. 2017; 7(1); 10377. [PubMed: 28871190].

2. Fu et al., 2012: Fu S, Zhang M, Ou J, Liu H, Tan C, Liu J, Chen H, Bei W. Construction and immune effect of Haemophilus parasuis DNA vaccine encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in mice. Vaccine. 2012; 30(48); 6839-6844. [PubMed: 23000128].

3. Guo et al., 2017: Guo L, Xu L, Wu T, Fu S, Qiu Y, Hu CA, Ren X, Liu R, Ye M. Evaluation of recombinant protein superoxide dismutase of Haemophilus parasuis strain SH0165 as vaccine candidate in a mouse model. Canadian journal of microbiology. 2017; 63(4); 312-320. [PubMed: 28177794].

4. Hong et al., 2011: Hong M, Ahn J, Yoo S, Hong J, Lee E, Yoon I, Jung JK, Lee H. Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis. Veterinary microbiology. 2011; 148(1); 89-92. [PubMed: 20817421].

5. Huang et al., 2013: Huang X, Li Y, Fu Y, Ji Y, Lian K, Zheng H, Wei J, Cai X, Zhu Q. Cross-protective efficacy of recombinant transferrin-binding protein A of Haemophilus parasuis in guinea pigs. Clinical and vaccine immunology : CVI. 2013; 20(6); 912-919. [PubMed: 23616407].

6. Li et al., 2015: Li M, Song S, Yang D, Li C, Li G. Identification of secreted proteins as novel antigenic vaccine candidates of Haemophilus parasuis serovar 5. Vaccine. 2015; 33(14); 1695-1701. [PubMed: 25704800].

7. Li et al., 2016: Li M, Li C, Song S, Kang H, Yang D, Li G. Development and antigenic characterization of three recombinant proteins with potential for Gl�sser's disease prevention. Vaccine. 2016; 34(19); 2251-2258. [PubMed: 26993332].

8. Li et al., 2017: Li M, Cai RJ, Song S, Jiang ZY, Li Y, Gou HC, Chu PP, Li CL, Qiu HJ. Evaluation of immunogenicity and protective efficacy of recombinant outer membrane proteins of Haemophilus parasuis serovar 5 in a murine model. PloS one. 2017; 12(4); e0176537. [PubMed: 28448603].

9. Macedo et al., 2016: Macedo N, Oliveira S, Torremorell M, Rovira A. Immune response to oligopeptide permease A (OppA) protein in pigs naturally and experimentally infected with Haemophilus parasuis. Research in veterinary science. 2016; 107; 62-67. [PubMed: 27473976].

10. Oliveira and Pijoan, 2004: Oliveira S, Pijoan C. Haemophilus parasuis: new trends on diagnosis, epidemiology and control. Veterinary microbiology. 2004; 99(1); 1-12. [PubMed: 15019107].

11. Olvera et al., 2011: Olvera A, Pina S, P�rez-Sim� M, Arag�n V, Segal�s J, Bensaid A. Immunogenicity and protection against Haemophilus parasuis infection after vaccination with recombinant virulence associated trimeric autotransporters (VtaA). Vaccine. 2011; 29(15); 2797-2802. [PubMed: 21320547].

12. Tian et al., 2011: Tian H, Fu F, Li X, Chen X, Wang W, Lang Y, Cong F, Liu C, Tong G, Li X. Identification of the immunogenic outer membrane protein A antigen of Haemophilus parasuis by a proteomics approach and passive immunization with monoclonal antibodies in mice. Clinical and vaccine immunology : CVI. 2011; 18(10); 1695-1701. [PubMed: 21832103].

13. Wen et al., 2016: Wen Y, Yan X, Wen Y, Cao S, He L, Ding L, Zhang L, Zhou P, Huang X, Wu R, Wen X. Immunogenicity of the recombinant HxuCBA proteins encoded by hxuCBA gene cluster of Haemophilus parasuis in mice. Gene. 2016; 591(2); 478-483. [PubMed: 27378742].

14. Yuan et al., 2012: Yuan F, Fu S, Hu J, Li J, Chang H, Hu L, Chen H, Tian Y, Bei W. Evaluation of recombinant proteins of Haemophilus parasuis strain SH0165 as vaccine candidates in a mouse model. Research in veterinary science. 2012; 93(1); 51-56. [PubMed: 21596404].

15. Zhou et al., 2009: Zhou M, Guo Y, Zhao J, Hu Q, Hu Y, Zhang A, Chen H, Jin M. Identification and characterization of novel immunogenic outer membrane proteins of Haemophilus parasuis serovar 5. Vaccine. 2009; 27(38); 5271-5277. [PubMed: 19576561].