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Pathogen Page

Bordetella bronchiseptica

Fold this section Table of Contents
Fold this section I. General Information
1. NCBI Taxonomy ID:
518
2. Disease:
Infectious bronchitis, kennel cough
3. Introduction
B. bronchiseptica displays a broad host range which includes mice, rats, guinea pigs, rabbits, cats, dogs, pigs, sheep, horses, and bears. Although human infections have been documented, they are usually associated with a severely compromised host. B. bronchiseptica causes a variety of respiratory diseases such as kennel cough in dogs, atrophic rhinitis in pigs and snuffles in rabbits. Infections established by this subspecies are typically chronic, often asymptomatic, and notoriously difficult to clear even with antibiotic therapy. B. bronchiseptica appears to occupy a position along a continuum with "pathogen" at one end and "commensal" at the other. Its ability to establish long-term asymptomatic infection seems to be an adaptive feature and may represent a balance between immunostimulatory events associated with infection and immunomodulatory events mediated by the bacteria. B. bronchiseptica infection of laboratory animals provides an excellent model system to understand mechanisms that promote persistent bacterial infections (Mattoo et al., 2001).
4. Host Ranges and Animal Models
B. bronchiseptica displays a broad host range which includes mice, rats, guinea pigs, rabbits, cats, dogs, pigs, sheep, horses, and bears. Although human infections have been documented, they are usually associated with a severely compromised host. Animal models for B. bronchiseptica have been developed that reflect both the natural course of infection as well as those that are specifically skewed toward disease. A B. bronchiseptica strain, RB50, has been isolated from the nose of a naturally infected New Zealand White rabbit. Specific pathogen free rabbits inoculated with RB50 become colonized in the nasal cavity, larynx, trachea and lungs. Rat and mouse models have also been developed (Mattoo et al., 2001). B. bronchiseptica has been isolated from dogs, humans, monkeys, cats, rabbits, ferrets, guinea pigs, mice,swine, foxes, rats, hedgehogs, horses, skunks, opossums, raccoons, koala bears, turkeys, and lesser bushbabies (Goodnow, 1980).
5. Host Protective Immunity
Prevention of B. bronchiseptica respiratory tract (lung, tracheal, or nasal turbinate) membrane infections of mammals appears to be dependent upon prevention of attachment to and colonization of host cells by invading bacteria. As there have been few reports of septicemicphase infections, the inhibition of these infections appears to be dependent upon localized activity of humoral agglutinins, antitoxins, or cellular immune factors (Goodnow, 1980).
1. aroA
  • Gene Name : aroA
  • Sequence Strain (Species/Organism) : Bordetella bronchiseptica RB50
  • NCBI Gene ID : 2659145
  • NCBI Protein GI : 33602442
  • Locus Tag : BB3469
  • Genbank Accession : BX640447
  • Protein Accession : NP_890002
  • Taxonomy ID : 257310
  • Gene Starting Position : 3712371
  • Gene Ending Position : 3713699
  • Gene Strand (Orientation) : -
  • Protein Name : 3-phosphoshikimate 1-carboxyvinyltransferase
  • Protein pI : 5.16
  • Protein Weight : 43401.08
  • Protein Length : 442
  • Protein Note : catalyzes the formation of 5-O-(1-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate and 3-phosphoshikimate in tryptophan biosynthesis
  • DNA Sequence : Show Sequence 
    >gi|33598993:3712371-3713699 Bordetella bronchiseptica RB50, complete genome
TTCAGTCCCGCGCGGCCAGCAGGCCCGCGTACACGTCGAAATAATCGGGGAAAGTCTTGCTGACGCAGCC
CGGATCCAGGATGCGCACCGCAGCCGGACCGAACGCGGCCAGCGAGAAGCACATGGCCATGCGGTGGTCG
TCCCAGGTGCCGATGTGGGCGTCGCGCCAGCCGCCGGGCGCCGGCGGCGCCACCTCCAGCCAGTCCGCCC
CGCTTTGCACGCCCGCCCCCAGCTTCTCCAGCTCGGTGTGCATGGCGTGGATGCGGTCGGTCTCCTTGAC
GCGCCAGCTGCCGATGTTGCGCAGGCGGCATGGGCCGTCGGCGTACAGCGCCAGCGTCGCGGCCGTCATG
GCGGCGTCGGGAATCAGGTTGAAGTCGGCGTCGAACGCCTTCAGGCGTCCGCCCTCGGCCACCCGCACGC
CGCGCGTCTCGATCCAGCCCGGGCCATAGCGCACGTCGGCGCCCATCGCCGCCAGCGTCGCGGCGAACGC
CACGTCGCCCTGGATGCTGTCCTCGCCCACGCCGGTGACGCGCACCGGCCCGCCGCCGATGGCGCCCAGG
GCCAGGAAGTACGACGCCGTCGAGGCATCGCCCTCGATCGCCATGCGGCCCGGGCCGCGGTAGGCCGCAT
CGCGCGCGATCGTGAAGGCGCGCCAGCCGTCGCGCCGCACCGACACGCCAAAACGCGCCATCAGATTGAG
CGTGATCTCGATATAGGGTTTGGAAATCAGCTCGCCCACCACCTCGATGGTGATGTCCTGGCCGCTGCGC
CGAGCCAGCACGGGGGCGGCCATCAGCAAGGCGGTCAGGAACTGGCTGGACACCGAGCCCTCCACCCGCA
CCGGCCCGTCGACGCGAATGCTGCCGCCGCCGATGCGCAGCGGCGGATACCCCGCCTGCCCCAGATATTC
GATCCCGGCGCCGAACTGGCGCAAGGCGTCGACCAGGTCGCCGATGGGCCGCTCGTGCATGCGCGGCACG
CCGGACAGGCGGTAATCGCCGCCCATCAACGCCAGCGCCGCGGTCAGCGGCCGGAACGCGGTGCCGGCGT
TGCCCAGGAACAGCTCGGCCTGTTCGGTCGGAAAGCGCGCCACGCCTTCGATGGTCACGCAGCCATCGGC
CACCTCGCCCACCGATACGCCCAGCTGGCGCAACGCGGCCAGCATGACGCGGGTGTCATCGGAATCGAGC
AGGCCCGTGATTTCGGTGCTGCCTTCGGCCAGCGCGGCCAGCAGCAATACCCTGTTGGAGATGCTCTTGG
AACCCGGCAGGGCCACCTCGCCGCGCGCCAGGCGCGCCGCGGGCAGGTCGAGATATGCCAATCCGCTCA
  • Protein Sequence : Show Sequence 
    >gi|33602442|ref|NP_890002.1| 3-phosphoshikimate 1-carboxyvinyltransferase [Bordetella bronchiseptica RB50]
MSGLAYLDLPAARLARGEVALPGSKSISNRVLLLAALAEGSTEITGLLDSDDTRVMLAALRQLGVSVGEV
ADGCVTIEGVARFPTEQAELFLGNAGTAFRPLTAALALMGGDYRLSGVPRMHERPIGDLVDALRQFGAGI
EYLGQAGYPPLRIGGGSIRVDGPVRVEGSVSSQFLTALLMAAPVLARRSGQDITIEVVGELISKPYIEIT
LNLMARFGVSVRRDGWRAFTIARDAAYRGPGRMAIEGDASTASYFLALGAIGGGPVRVTGVGEDSIQGDV
AFAATLAAMGADVRYGPGWIETRGVRVAEGGRLKAFDADFNLIPDAAMTAATLALYADGPCRLRNIGSWR
VKETDRIHAMHTELEKLGAGVQSGADWLEVAPPAPGGWRDAHIGTWDDHRMAMCFSLAAFGPAAVRILDP
GCVSKTFPDYFDVYAGLLAARD
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Inactivation of the aroA gene highly attenuates B. bronchiseptica, severely impairing its ability to colonize and survive in the respiratory tract of mice (Stevenson and Roberts, 2002).
  • Related Vaccine(s): Bordetella bronchiseptica aroA mutant vaccine (strain BBS18) , Bordetella bronchiseptica aroA/trpE mutant vaccine
2. bscN
  • Gene Name : bscN
  • Sequence Strain (Species/Organism) : Bordetella bronchiseptica RB50
  • NCBI Gene ID : 2662485
  • NCBI Protein GI : 33600613
  • Locus Tag : BB1628
  • Genbank Accession : BX640441
  • Protein Accession : NP_888173
  • Taxonomy ID : 257310
  • Gene Starting Position : 1729444
  • Gene Ending Position : 1730778
  • Gene Strand (Orientation) : +
  • Protein Name : type III secretion system ATPase
  • Protein pI : 6.86
  • Protein Weight : 43916.31
  • Protein Length : 444
  • Protein Note : ortholog of Bordetella pertussis (BX470248) BP2245
  • DNA Sequence : Show Sequence 
    >gi|33598993:1729444-1730778 Bordetella bronchiseptica RB50, complete genome
CATGCGTCAGTACCACTACATCACGGAGATGATGCGGGTGGCCCTGCAGGATCTGTCCACGTTGCGGATA
AAGGGCCGAGTGGTGCAAGTGGTGGGAACGATCATCAAGGCCGTCGTTCCGATGGTCAAGATCGGCGAAG
TGTGCCTGCTGCGCAATCCTGGCGAGGACTTCGAGATGCACGGCGAAGTGGTGGGCTTTGTTCGCGACGC
CGCCTTGCTCACGCCCATCGGCGACATGTACGGGATTTCCTCGGCGACCGAGGTGATACCGACCGGACGC
ACGCATATGGTGCCCGTCGGTCCGGGCTTGCTGGGACGCGTGCTGGACGGGCTGGGACGTCCGCTGGACG
TCGCCGAGTCAGGGCCGCTGCATGCCCACAAGTTCTATCCGGTCTTCGCCGATGCGCCGGATCCGCTGAC
GCGTCGCATCATCCATGCTCCGCTGGAGCTGGGGGTGCGCGTACTGGACGGTTTGCTTACATGCGGGGAA
GGCCAGCGTCTGGGAATTTTCGCAGCAGCCGGCGGCGGCAAGTCGACCTTGCTGGGCATGCTGGTCAAGG
GCGCCGCGGTCGACGTGACGGTGGTGGCGCTGATCGGCGAGCGTGGGCGGGAAGTTCGCGAGTTCCTTGA
GCACGAACTCGGTCCGGAGGGCAGACGCAAGAGCGTGATCGTCTGCGCGACCAGCGACAAGTCCTCGATG
GAGCGTGCCAAGGCGGCATACGTCGCAACCGCCATCGCCGAATACTTCCGCGATCAAGGGCAGCGTGTAC
TTTTTCTGATGGACTCGGTCACCCGCTTTGCGCGAGCCCAGCGTGAAATCGGCTTGGCGGCAGGCGAGCC
GCCGACGCGGCGCGGCTATCCGCCGTCGGTGTTCGCCACCCTGCCAAAACTGATGGAGCGCGCCGGCATG
AACCAGACGGGTTCGATCACGGCGCTGTATACGGTGCTGGTCGAGGGGGACGACATGAACGAACCGGTGG
CCGACGAGACGCGTTCGATACTGGACGGCCACATCGTGCTCTCGCGCAAGCTGGGAGCGGCGAATCACTA
TCCTGCCGTCGACGTGCTGGCCTCAGCCAGCCGGGTCATGAATGCCGTGGTGTCGCCACGTCACAAGTAC
CTGGCCGGACGTATGCGCGAACTGATGGCCAAGTACCAGGACGTCGAGCTGTTGGTGAAAATCGGCGAGT
ACAAGCAGGGCGCCGATGCGTCGACCGATGAGGCGATACAGAAGATCGGACAGATCAATGCGTTTCTCAG
ACAACTAACCGACGAACGCGAAGCATTCGAGGATACCGTACTGCGCATGGCTGAAATCATCGGACCCGAA
TCCTA
  • Protein Sequence : Show Sequence 
    >gi|33600613|ref|NP_888173.1| type III secretion system ATPase [Bordetella bronchiseptica RB50]
MRQYHYITEMMRVALQDLSTLRIKGRVVQVVGTIIKAVVPMVKIGEVCLLRNPGEDFEMHGEVVGFVRDA
ALLTPIGDMYGISSATEVIPTGRTHMVPVGPGLLGRVLDGLGRPLDVAESGPLHAHKFYPVFADAPDPLT
RRIIHAPLELGVRVLDGLLTCGEGQRLGIFAAAGGGKSTLLGMLVKGAAVDVTVVALIGERGREVREFLE
HELGPEGRRKSVIVCATSDKSSMERAKAAYVATAIAEYFRDQGQRVLFLMDSVTRFARAQREIGLAAGEP
PTRRGYPPSVFATLPKLMERAGMNQTGSITALYTVLVEGDDMNEPVADETRSILDGHIVLSRKLGAANHY
PAVDVLASASRVMNAVVSPRHKYLAGRMRELMAKYQDVELLVKIGEYKQGADASTDEAIQKIGQINAFLR
QLTDEREAFEDTVLRMAEIIGPES
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A double mutant strain of Bordetella Bronchiseptica lacking adenylate cyclase(cyaA gene) and type III secretion(bscN gene) is attenuated and provides protection in mice against 5 × 10^5 CFU of RB50, the B. Bronchiseptica parent strain (Mann et al., 2007).
  • Related Vaccine(s): Bordetella bronchiseptica bscN and cyaA double mutant vaccine
3. cyaA
  • Gene Name : cyaA
  • Sequence Strain (Species/Organism) : Bordetella bronchiseptica RB50
  • NCBI Gene ID : 2661688
  • NCBI Protein GI : 33599313
  • Locus Tag : BB0324
  • Genbank Accession : BX640437
  • Protein Accession : NP_886873
  • Taxonomy ID : 257310
  • Gene Starting Position : 331399
  • Gene Ending Position : 336621
  • Gene Strand (Orientation) : +
  • Protein Name : bifunctional hemolysin-adenylate cyclase precursor
  • Protein pI : 4.4
  • Protein Weight : 167791.35
  • Protein Length : 1740
  • Protein Note : Also known as cyaortholog of Bordetella pertussis (BX470248) BP0760
  • DNA Sequence : Show Sequence 
    >gi|33598993:331399-336621 Bordetella bronchiseptica RB50, complete genome
CGTGCTGGATGTTTGGTTCTTGCAGAAGGATGAGGTTCTGAGCGCTACACACCGGTTGCGTCGGTGCGAA
TCCGTTCAATCGACTACTTATCGACAGATCCACATGCAGCAATCGCATCAGGCTGGTTACGCAAACGCCG
CCGACCGGGAGTCTGGCATCCCCGCAGCCGTACTCGATGGCATCAAGGCCGTGGCGAAGGAAAAAAACGC
CACATTGATGTTCCGCCTGGTCAACCCCCATTCCACCAGCCTGATTGCCGAAGGGGTGGCCACCAAAGGA
TTGGGCGTGCACGCCAAGTCGTCCGATTGGGGGTTGCAGGCGGGCTACATTCCCGTCAACCCGAATCTTT
CCAAACTGTTCGGCCGTGCGCCCGAGGTGATCGCGCGGGCCGACAACGACGTCAACAGCAGCCTGGCGCA
TGGCCATACCGCGGTCGACCTGACGCTGTCGAAAGAGCGGCTTGACTATCTGCGGCAAGCGGGCCTGGTC
ACCGGCATGGCCGATGGCGTGGTCGCGAGCAACCACGCAGGCTACGAGCAGTTCGAGTTTCGCGTGAAGG
AAACCTCGGACGGGCGCTATGCCGTGCAGTATCGCCGCAAGGGCGGCGACGATTTCGAGGCGGTCAAGGT
GATCGGCAATGCCGCCGGTATTCCACTGACGGCGGATATCGACATGTTCGCCATCATGCCGCATCTGTCC
AACTTCCGCGACTCGGCGCGCAGTTCGGTGACCAGCGGCGATTCGGTGACCGATTACCTGGCGCGCACGC
GGCGGGCCGCCAGCGAGGCCACGGGCGGCCTGGATCGCGAACGCATCGACTTGTTGTGGAAAATCGCTCG
CGCCGGCGCCCGTTCCGCAGTGGGCACCGAGGCGCGTCGCCAGTTCCGCTACGACGGCGACATGAATATC
GGCGTGATCACCGATTTCGAGCTGGAAGTGCGCAATGCGCTGAACAGGCGGGCGCACGCCGTCGGCGCGC
AGGACGTGGTCCAGCATGGCACTGAGCAGAACAATCCTTTCCCGGAGGCAGATGAGAAGATTTTCGTCGT
ATCGGCCACCGGTGAAAGCCAGATGCTCACGCGCGGGCAACTGAAGGAATACATTGGCCAGCAGCGCGGC
GAGGGCTATGTCTTCTACGAGAACCGTGCGTACGGCGTGGCGGGGAAAAGCCTGTTCGACGATGGGCTGG
GAGCCGCGCCCGGCGTGCCGGGCGGACGTTCGAAGTCCTCGCCGGATGTACTGGAAACGGTGCCGGCGTC
ACCCGGATTGCGGCGGCCGTCGCTGGGCGCAGTGGAACGCCAGGATTCCGGCTATGACAGCCTTGATGGG
GTGGGATCGCGATCGTTCTCGTTGGGCGAGGTGTCCGACATGGCCGCCGTGGAAGCGGCGGAACTGGAAA
TGACCCGGCAAGTCTTGCACGCCGGGGCGCGGCAGGACGATGCCGAGCCGGGCGTGAGCGGTGCGTCGGC
GCACTGGGGGCAGCGGGCGCTGCAGGGCGCCCAGGCGGTGGCGGCGGCGCAGCGGCTGGTTCATGCCATT
GCCCTGATGACGCAATTCGGCCGGGCCGGTTCCACCAACACGCCGCAGGAAGCGGCCTCGTTGTCGGCGG
CCGTGTTCGGCTTGGGCGAGGCCAGCAGCGCCGTGGCCGAAACCGTGAGCGGTTTTTTCCGCGGGTCTTC
GCGCTGGGCCGGCGGTTTCGGCGTGGCTGGCGGCGCGATGGCGCTGGGAGGCGGCATCGCCGCGGCCGTT
GGCGCCGGGATGTCGTTGACCGATGACGCGCCGGCCGGACAGAAGGCCGCCGCCGGCGCCGAGATCGCGC
TGCAGTTGACAGGTGGAACGGTCGAGCTGGCTTCTTCCATCGCGTTGGCGCTGGCCGCGGCGCGCGGCGT
GACCAGCGGCTTGCAGGTGGCGGGGGCGTCGGCCGGGGCGGCTGCCGGCGCATTGGCCGCGGCGCTCAGT
CCCATGGAGATCTACGGCCTGGTGCAGCAATCGCACTATGCGGATCAGCTGGACAAGCTGGCGCAGGAAT
CGAGCGCATACGGTTACGAGGGCGACGCCTTGCTGGCCCAGCTGTATCGCGACAAGACGGCCGCCGAGGG
CGCCGTCGCCGGCGTCTCCGCCGTCCTGAGCACGGTGGGGGCTGCGGTGTCGATCGCCGCGGCGGCCAGC
GTGGTAGGCGCCCCGGTGGCGGTGGTCACTTCCTTGTTGACCGGGGCTCTCAACGGCATCCTGCGCGGCG
TGCAGCAGCCCATCATCGAAAAGCTGGCCAATGATTACGCTCGCAAGATCGACGAGCTGGGCGGGCCGCA
AGCGTACTTCGAGAAAAACCTGCAGGCGCGTCACGAACAACTGGCCAATTCGGACGGCCTACGGAAAATG
CTGGCCGACCTGCAGGCCGGGTGGAACGCCAGCAGCGTGATCGGGGTGCAGACGACAGAGATTTCCAAGT
CGGCGCTCGAACTGGCCGCCATTACCGGCAACGCGGACAACCTGAAATCCGCCGACGTGTTCGTGGACCG
CTTCATCCAGGGCGAGCGGGTGGCCGGCCAGCCGGTGGTACTCGACGTCGCCGCCGGCGGCATCGATATC
GCCAGCCGCAAGGGCGAGCGGCCGGCGCTGACGTTCATCACGCCGCTGGCCGCGCCAGGAGAAGAGCAGC
GCCGGCGCACGAAAACGGGCAAGAGCGAATTCACCACATTCGTCGAGATCGTGGGCAAGCAGGACCGCTG
GCGCATCCGGGACGGCGCGGCCGACACCACCATCGATCTGGCCAAGGTGGTGTCGCAACTGGTCGACGCC
AATGGCGTGCTCAAGCACAGCATCAAACTGGAGGTGATCGGCGGAGATGGCGATGATGTCGTGCTTGCCA
ATGCTTCGCGCATCCATTACGACGGCGGCGCGGGAACCAACACGGTCAGCTATGCCGCCCTGGGCCGACA
GGATTCCATTACCGTGTCCGCCGACGGGGAACGTTTCAACGTGCGCAAGCAGTTGAACAACGCCAACGTG
TATCGCGAAGGCGTGGCTACCCAGAAAACCGCCTACGGCAAGCGCACGGAGAATGTCCAATACCGCCATG
TCGAGCTGGCCCGTGTCGGGCAACTGGTGGAGGTCGACACGCTCGAGCATGTGCAGCACATCATCGGCGG
GGCCGGCAACGATTCGATCACCGGCAATGCGCACGACAACTTCCTGGCCGGCGGGGCGGGCGACGACAGG
CTGGATGGCGGCGCCGGCAACGACACACTGGTCGGCGGCGAGGGCCACAACACGGTCGTCGGCGGCGCTG
GCGACGACGTATTCCTGCAGGACCTGGGGGTATGGAGCAACCAGCTCGATGGCGGCGCGGGCGTCGATAC
CGTGAAGTACAACGTGCACCAGCCTTCCGAGGAACGCCTCGAACGCATGGGCGACACGGGCATCCATGCC
GATCTTCAAAAGGGCACGGTCGAGAAGTGGCCGGCCCTGAACCTGTTCAGCGTCGACCATGTCAAGAATA
TCGAGAATCTGCACGGCTCCAGCCTGAACGACAGCATCGCCGGCGACGACCGGGACAACGAGCTCTGGGG
CGACGATGGCAACGACACGATACACGGCCGGGGCGGCGACGATATCCTGCGCGGCGGCCTGGGCCTGGAC
ACGCTGTATGGCGAGGACGGCAACGACATCTTCCTGCAGGACGACGAGACCGTCAGCGATGACATCGACG
GTGGCGCGGGACTGGACACCGTCGACTATTCCGCCATGATCCATGCAGGCAAGATCGTTGCGCCGCATGA
ATACGGCTTCGGGATCGAGGCGGACCTGTCCGAAGGGTGGGTGCGCAAGGCGGCCCGGCGCGGCATGGAC
TACTACGACAGTGTCCGCAGTGTCGAAAACGTCATCGGCACGAGCATGAAGGATGTGCTCATCGGCGACG
CGCAAGCCAATACCCTGATGGGCCAGGGCGGCGACGATACCGTGCGCGGCGGCGACGGCGATGATCTGCT
GTTCGGCGGCGACGGCAACGACATGCTGTATGGAGACGCCGGCAACGACACCCTCTACGGAGGGCTGGGC
GACGATACCCTTGAAGGCGGCGCGGGCAACGATTGGTTCGGCCAGACGCCGGCGCGCGAGCATGACGTGC
TGCGCGGCGGGGCTGGGGTGGATACCGTGGATTACAGCCAGGCGGGCGCGCATGCCGGCGTTGCCACGGG
TCGCATCGGGCTGGGTATTCTGGCGGACCTGGGCGCCGGCCGCGTCGACAAGCTGGGCGAGGCCGGCAGC
AGCGCCTACGATACGGTTTCCGGCATCGAAAATGTGGTGGGCACGGAACTGGCCGACCGCATCACGGGCG
ATGCGCAGGCCAACGTACTGCGCGGCGCGGGTGGCGCCGACGTGCTTGCGGGCGGCGAGGGCGACGATGT
GCTGCTGGGCGGCGACGGCGACGACCAGCTGTCGGGCGACGCCGGACGCGACCGCTTGTACGGCGAAGCC
GGTGACGACTGGTTCTTCCAGGATGCCGCCAATGCCGGCAATCTGCTCGACGGTGGTGACGGCAACGATA
CCGTGGATTTCAGCGGCCCGGGCCGGGGCCTCGACGCCGGCGCAAAGGGCGTATTCCTGAGCCTGGGCAA
GGGGTTCGCCAGCCTGATGGACGAACCCGAAACCAGCAACGTGTTGCGCCATATCGAGAACGCCGTGGGC
AGCGTGCGTGATGACGTGCTGATCGGCGACGCAGGCGCCAACGTCCTCAATGGCCTGGCGGGCAACGACG
TGCTGTCCGGCGGCGCCGGCGACGATGTGCTGCTGGGCGACGAGGGCTCGGACCTGCTCAGCGGCGATGC
GGGCAACGACGATCTGTTCGGCGGGCAGGGCGATGATACCTATCTGTTCGGGGCCGGGTACGGACATGAC
ACGATCTACGAATCGGGCGGCGGCCATGACACCATCCGTATCAACGCGGGGGCGGACCAGCTGTGGTTTG
CGCGCCAGGGCAACGACCTGGAGATCCGCATTCTTGGCACCGACGATGCACTTACCGTGCACGACTGGTA
TCGCGACGCCGATCACCGGGTGGAAGCCATCCATGCCGCCAACCAGGCCATAGACCCGGCCGGCATCGAA
AAGCTGGTCGAGGCAATGGCGCAGTACCCGGACCCCGGCGCGGCGGCGGCTGCCCCGCCGGCGGCGCGCG
TGCCGGACACGCTGATGCAGTCCCTGGCTGTCAACTGGCGCTG
  • Protein Sequence : Show Sequence 
    >gi|33599313|ref|NP_886873.1| bifunctional hemolysin-adenylate cyclase precursor [Bordetella bronchiseptica RB50]
MLDVWFLQKDEVLSATHRLRRCESVQSTTYRQIHMQQSHQAGYANAADRESGIPAAVLDGIKAVAKEKNA
TLMFRLVNPHSTSLIAEGVATKGLGVHAKSSDWGLQAGYIPVNPNLSKLFGRAPEVIARADNDVNSSLAH
GHTAVDLTLSKERLDYLRQAGLVTGMADGVVASNHAGYEQFEFRVKETSDGRYAVQYRRKGGDDFEAVKV
IGNAAGIPLTADIDMFAIMPHLSNFRDSARSSVTSGDSVTDYLARTRRAASEATGGLDRERIDLLWKIAR
AGARSAVGTEARRQFRYDGDMNIGVITDFELEVRNALNRRAHAVGAQDVVQHGTEQNNPFPEADEKIFVV
SATGESQMLTRGQLKEYIGQQRGEGYVFYENRAYGVAGKSLFDDGLGAAPGVPGGRSKSSPDVLETVPAS
PGLRRPSLGAVERQDSGYDSLDGVGSRSFSLGEVSDMAAVEAAELEMTRQVLHAGARQDDAEPGVSGASA
HWGQRALQGAQAVAAAQRLVHAIALMTQFGRAGSTNTPQEAASLSAAVFGLGEASSAVAETVSGFFRGSS
RWAGGFGVAGGAMALGGGIAAAVGAGMSLTDDAPAGQKAAAGAEIALQLTGGTVELASSIALALAAARGV
TSGLQVAGASAGAAAGALAAALSPMEIYGLVQQSHYADQLDKLAQESSAYGYEGDALLAQLYRDKTAAEG
AVAGVSAVLSTVGAAVSIAAAASVVGAPVAVVTSLLTGALNGILRGVQQPIIEKLANDYARKIDELGGPQ
AYFEKNLQARHEQLANSDGLRKMLADLQAGWNASSVIGVQTTEISKSALELAAITGNADNLKSADVFVDR
FIQGERVAGQPVVLDVAAGGIDIASRKGERPALTFITPLAAPGEEQRRRTKTGKSEFTTFVEIVGKQDRW
RIRDGAADTTIDLAKVVSQLVDANGVLKHSIKLEVIGGDGDDVVLANASRIHYDGGAGTNTVSYAALGRQ
DSITVSADGERFNVRKQLNNANVYREGVATQKTAYGKRTENVQYRHVELARVGQLVEVDTLEHVQHIIGG
AGNDSITGNAHDNFLAGGAGDDRLDGGAGNDTLVGGEGHNTVVGGAGDDVFLQDLGVWSNQLDGGAGVDT
VKYNVHQPSEERLERMGDTGIHADLQKGTVEKWPALNLFSVDHVKNIENLHGSSLNDSIAGDDRDNELWG
DDGNDTIHGRGGDDILRGGLGLDTLYGEDGNDIFLQDDETVSDDIDGGAGLDTVDYSAMIHAGKIVAPHE
YGFGIEADLSEGWVRKAARRGMDYYDSVRSVENVIGTSMKDVLIGDAQANTLMGQGGDDTVRGGDGDDLL
FGGDGNDMLYGDAGNDTLYGGLGDDTLEGGAGNDWFGQTPAREHDVLRGGAGVDTVDYSQAGAHAGVATG
RIGLGILADLGAGRVDKLGEAGSSAYDTVSGIENVVGTELADRITGDAQANVLRGAGGADVLAGGEGDDV
LLGGDGDDQLSGDAGRDRLYGEAGDDWFFQDAANAGNLLDGGDGNDTVDFSGPGRGLDAGAKGVFLSLGK
GFASLMDEPETSNVLRHIENAVGSVRDDVLIGDAGANVLNGLAGNDVLSGGAGDDVLLGDEGSDLLSGDA
GNDDLFGGQGDDTYLFGAGYGHDTIYESGGGHDTIRINAGADQLWFARQGNDLEIRILGTDDALTVHDWY
RDADHRVEAIHAANQAIDPAGIEKLVEAMAQYPDPGAAAAAPPAARVPDTLMQSLAVNWR
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A double mutant strain of Bordetella Bronchiseptica lacking adenylate cyclase(cyaA gene) and type III secretion(bscN gene) is attenuated and provides protection in mice against 5 × 10^5 CFU of RB50, the B. Bronchiseptica parent strain (Mann et al., 2007).
  • Related Vaccine(s): Bordetella bronchiseptica bscN and cyaA double mutant vaccine
4. dnt
  • Gene Name : dnt
  • Sequence Strain (Species/Organism) : Bordetella bronchiseptica RB50
  • NCBI Gene ID : 2661538
  • NCBI Protein GI : 33602952
  • Locus Tag : BB3978
  • Genbank Accession : BX640449
  • Protein Accession : NP_890512
  • Taxonomy ID : 257310
  • Gene Starting Position : 4225249
  • Gene Ending Position : 4229643
  • Gene Strand (Orientation) : -
  • Protein Name : dermonecrotic toxin
  • Protein pI : 6.6
  • Protein Weight : 150178.45
  • Protein Length : 1464
  • Protein Note : ortholog of Bordetella pertussis (BX470248) BP3439
  • DNA Sequence : Show Sequence 
    >gi|33598993:4225249-4229643 Bordetella bronchiseptica RB50, complete genome
TTCAGACCGGCGCCGGAAACAACGTCACCAATCGTTCGTTCAACACATCCAAGGCACTGCCGCTGCCTGC
CCAGGCCAGATCCTCCGCCAGCAGGACGGCTCGCGCCCCTTGTCCGTCGTTGGAAATCAGAACGTATACC
TGGCCGAGGCGGGGAACGGCCGACGCCTGGACTGCTGCGTTGTAGTCGAACGAAACCACACTGCCCGCCC
CCGCATCGTGACGCGTTATGCGCGTACTGCCGCCTCCCGGCACATCCTTGCCCAGATAGGCAATGACCGC
CCGGTCGTAGGTCGCGGCGATGCTGACCAAGTCGTCATTGCGCATCGGCGCCGGGATTGAGATCGGCGCG
TACCCCATCGCCAAATGGGCCTGGTAAAGCGCCTGTACGCCTTCGCGCGCAGTCGCCCAATCCCCGAGTT
CGGTCGACTTGCCAGTGTGGTAGAAGGCCAGGTAGCCCTCCTTGACCCCAACCATCGTCGTGCACCCGCT
CAAGGAGCCGCCAGTCAGGATGAGCGGCGTCGCGACGGGAACAGTGTCGAGCCTCACGCGCACCCCGATC
GCCCCTTCATCGCCGTTCTCGATGCTGATGATCCGCACATTGTCGGTCAGCATGACCTCGCGTGCCGCTG
CGGGCGCGCCGTACGCATATTCGATCTCAGCCCCCGTCCACGATTGAATATAGCCATGGACAGGCCCGTC
GACCGGCCTGAAGACGCTGGCGTCGAGCGCACGCGGCGCGGACATGCGCACCGGGCGGTCGTAACGCACG
AACGAAATATCCAGCCCCGGAGGAAGATCGATGTCGCGCCTGCCGCCCAATGGCGGCCTGGCGCGCAGCG
GGGAAATATCCCCATGGTTGAGCAATCCGATATCGGTATTTGCGTAATCCAGGCGCGTCGGGCTGATGGC
GGCAGGACCGAAGTTCCATACCAGCGGCCCCCCGCCGGAAGCCTGTCGCCATGCGGGAAATATATCGTTC
ACGAACGACTGGAATATCGCGGGCGACGATTCCGAATAGGCGCCCAGCATGACGAAAAGACCGGTGCTGG
CGGGATTCCCGATACCGCGTTCGAGCTGGGCGACGTTCGAGATATCGAACACATTGCCCAGCAGCCGGGG
CGAAACGGCCTCCACGACCTCACTGGCCGTCGCGGCATGGCCCAGGCTGAGCAGGTGGAACGCCATCAGG
TCGACAAAACGCGCATTTTCATAGCCGAGCCTGGCATGCAAATCGACAATGGCATCCAGATACAACTCCA
TCCGAGCCTGCCTGCCCAGCGACGTGTTCCGGTCCACTGCGTTAAACAGCGTTGCGACATCGTCGAGGTC
ATATCCCGCCCGGTACAGCACGTCATTGACCGGACTCAGATCGCCGATATAGGCCCTGACCTCCTGCAGG
CGTCGCCCGATGCGCTGGCGAAGGCCGGCGTCCGGCTCGCCGAAGAAAATCCGGATCGCCGCGTCCATAT
CGGATTCAGCGCGCGGCGACGCAAGCCTGCTCCTGCAGGTATCGATATGCCTGAGCGCCACACGCCTCGC
CTGGTTGAGCAGGCGGCGCATCGGCGCGGCCTGCTCGGCGGCAAGGGCGACCAGATTACGCGTGCGGATG
CGTCCGAAGATTTCCGGCGCCCCACCCCGGCCAGGGACATCCAGATGCGTCTCCCAGTGGCCTTGCCCGT
TCAGCCGAACCGGAACGGTATAGGTCAGCCTCGGCGGCAAGCGCCCATTGACCACGCGCCAACCATGCGT
GTATGCATCCCAGATGACTCTGTAGGCATTCCCGGATTCGACGATGAAGTACTCGGTGCGCTCGCTCAAC
GGTCCCAAGCCGCGCGTGTAGATGCCCTCAAACCTGCCGCCCGACGTGGACTCGACCAAACCGGTGGCGT
CCACGCCGGCCATCCGCCATTCGCTTGCCAATTGGCCCCCCGTGCGCCCGGCCGAGGAACTGGCAGCGGA
GAATGCCTGCCGCAGGAAGGCCTGGTTACGCCCGCCGACCGTAGTGGCGCTCACGAATCCCGCGACAAGC
CGACGGATCGGCACGGGCTCGAAGATCTCATACAGATCGACGAGCAGCGAACGCGACAGGCGCAACCCGC
TGGCTCCGATCAATGCCGGAAGCTCCTTGAGCACCGAACGCCACAAGCGCTGCCCGCTCAACCCCGCGAT
GCGTTGCCGCACCACGAAATTGCGCAGGTTGCCATACTGGGTACGGGTGAAACTGCCCAACTTCCCTCCT
ATGCTGAACACCGAGGCAAGCAAGGAAATCGAATCAAAGACGATGCTCCCCACATCGACCCGATAGGAAC
GATCCTGGGTCGAAAAGAATATTTCCCGGTAGAACGGCACGATCCAGAAGGCGAACTTCTGCCATTCGCT
GGGTTTGTACAAAGCGGACTTCGCGTGCGCCGCGAGGTCGTCCAGACTGTGCCTCAGGGCAACGGAAACA
GCGAATAGCGGCACACCCAGCGCGCCTGCGGGCAGCCTGTACTCTTGCCGATACCCGAAATACCAGCATT
TCTTGACATCCAGCCCCGGATTGCTCTGCCTCAATTTGAGGAGGTAGTTGTCGCGCAGACTTGGCCCTTC
AGTCGCGGGGCCGCCGAGAAACCACCCGCCCGTTCGACGGCCGGTTCGAAAGCGGCCTTGCGGCTCGGCA
AGGGCGGCCAGCGTAGGATTCTGCATCACCTCAGCGTTCGCAAGGACATCAGACCAAAGCTCACCGTTGA
TGTTCGATACCAACAGCCAGCTGGAATCATGGAGCTGGAACATGGTGAAAAGACCCGGCAGATCGGTCAG
CGGCGGCCGCCCCAGGCAATATTCAATGCCCCTGTTGTGATTTTCGATTCCACGCGCGATCAGCCCTGGC
TGCGATGTCAGCACCTTGTAGTGCCGTACTTTCTGCAGGGGAAGGCACCATTGATCGACCGTCATCCGCG
AGCCCAGGGCCAGAATCCGCGCCAGCCGCTGCGCCTCGAGTATGCTGTATTGATCGATATAGTCGTTGAA
CTGGGCCGCATACTCCGCGTAGCTGGCGAACGCGTCGCTATCGCGAAAATCGGTAAAACTGATGAAGCCC
CGAGGCTCCGGCTCCTGGTCGCCAAGCAGCATGCCCGCGGTTTCCTGCTCCAGCCTTCTGAATCGCGCAA
GCACGCCAAGGGGCCGAAACCTGGCCAATACGACGGGCAACGTGCGCTGCGCGCTCTCGAGATCGCAGAG
CACGCCCGCGATCAACGCGGCGGCCATGGATACATAAGCCGCGTCATTGGCTGCTCCACTGGCCACGATT
TCGTCGTACTCGCCAGCCGCAACCGCCGGCATCGTAGAGGCGTTGCCGCGCCGCGCCCCCAGCAACCGCA
AGGCCTCGCGCTTGATGAACCGAAGAGCCAGCGCATTGCGGTAATCGCCGTAGACGCGCACATCGCGCAA
GGCGGCGTAGTCGAGCGCGAACGCCGCCTCGAGCAGATCAACCCGCGCCTGCTGGGTCGCGGCCAGATGA
AACTTGGCGATTTCCCACGCATCAAGTTTGCGTGGCATCTCGTCTCCCGGGCTATCGACCGAGCCTTGCG
CCCGGATGCGCAGTTGGGCATGCAGGCAATCCGCCAATGCATCGGCATACGCCAGATCGGCAACGGTGCG
CATATACCCTGCATCCGCGACCACGCCATATGGGCCGGATGCCTGGAAAATCGCCTGCTCGCCCCGATTG
ATGCCGTCGCGGCACAAACGCCATACGTCGTGTTCGGCGGTATCCAGACTGCCGTCGTCCGGCTCTCCGT
CCATGCGGCGTTCGATGCTTTCGGCGAGCGCCTGCTCGTAGATGTCGGCATCCAGATCGACGGTGCCGGC
CAATTCGTCGTAGGCGAACCATAGCGGGTGTTCGAACACCGAGCCCAGACGTCCCGAACGATCCACCGGA
CGCTGGCGGCGGCGCTTGAGGTACGCCGCCGCCAGCGGATGCGGCACGCGATTCAGTAGGTAGTAGATCT
CGTAGCGCGCCTTGACATACTCGAATACCAGGTCGCTGATCGCAGTGCGGGACAAGGCGGCGATCAACAC
GCCGAGATAGACATCGAGTGCGATCAACCACTGCAGGATCAATGACTCAGGCTCGAGACGAGCACGAATC
GCCACCTGCATTTTCTTCAAATTGGAAACCGCCACCGGGCGGTACTTGGGATAATAGAACCATGTACGAG
CATCCTGGCCGATGGGTTCATCCCCGTTGGGCGCTTCGCTAAACAGCGCGAATTCGGCCTTCTCTCGTTT
GGCCCGTCCACCCGCAAGATCCCCGGACTCTTCCGAGGGCAGCGCCAGCAACTCTTCCACCACGCCGTCG
TAGCCTACAAGCGCCATGTCGACAAGTTGCCGCAATGCCGATTCATCTTTATCCA
  • Protein Sequence : Show Sequence 
    >gi|33602952|ref|NP_890512.1| dermonecrotic toxin [Bordetella bronchiseptica RB50]
MDKDESALRQLVDMALVGYDGVVEELLALPSEESGDLAGGRAKREKAEFALFSEAPNGDEPIGQDARTWF
YYPKYRPVAVSNLKKMQVAIRARLEPESLILQWLIALDVYLGVLIAALSRTAISDLVFEYVKARYEIYYL
LNRVPHPLAAAYLKRRRQRPVDRSGRLGSVFEHPLWFAYDELAGTVDLDADIYEQALAESIERRMDGEPD
DGSLDTAEHDVWRLCRDGINRGEQAIFQASGPYGVVADAGYMRTVADLAYADALADCLHAQLRIRAQGSV
DSPGDEMPRKLDAWEIAKFHLAATQQARVDLLEAAFALDYAALRDVRVYGDYRNALALRFIKREALRLLG
ARRGNASTMPAVAAGEYDEIVASGAANDAAYVSMAAALIAGVLCDLESAQRTLPVVLARFRPLGVLARFR
RLEQETAGMLLGDQEPEPRGFISFTDFRDSDAFASYAEYAAQFNDYIDQYSILEAQRLARILALGSRMTV
DQWCLPLQKVRHYKVLTSQPGLIARGIENHNRGIEYCLGRPPLTDLPGLFTMFQLHDSSWLLVSNINGEL
WSDVLANAEVMQNPTLAALAEPQGRFRTGRRTGGWFLGGPATEGPSLRDNYLLKLRQSNPGLDVKKCWYF
GYRQEYRLPAGALGVPLFAVSVALRHSLDDLAAHAKSALYKPSEWQKFAFWIVPFYREIFFSTQDRSYRV
DVGSIVFDSISLLASVFSIGGKLGSFTRTQYGNLRNFVVRQRIAGLSGQRLWRSVLKELPALIGASGLRL
SRSLLVDLYEIFEPVPIRRLVAGFVSATTVGGRNQAFLRQAFSAASSSAGRTGGQLASEWRMAGVDATGL
VESTSGGRFEGIYTRGLGPLSERTEYFIVESGNAYRVIWDAYTHGWRVVNGRLPPRLTYTVPVRLNGQGH
WETHLDVPGRGGAPEIFGRIRTRNLVALAAEQAAPMRRLLNQARRVALRHIDTCRSRLASPRAESDMDAA
IRIFFGEPDAGLRQRIGRRLQEVRAYIGDLSPVNDVLYRAGYDLDDVATLFNAVDRNTSLGRQARMELYL
DAIVDLHARLGYENARFVDLMAFHLLSLGHAATASEVVEAVSPRLLGNVFDISNVAQLERGIGNPASTGL
FVMLGAYSESSPAIFQSFVNDIFPAWRQASGGGPLVWNFGPAAISPTRLDYANTDIGLLNHGDISPLRAR
PPLGGRRDIDLPPGLDISFVRYDRPVRMSAPRALDASVFRPVDGPVHGYIQSWTGAEIEYAYGAPAAARE
VMLTDNVRIISIENGDEGAIGVRVRLDTVPVATPLILTGGSLSGCTTMVGVKEGYLAFYHTGKSTELGDW
ATAREGVQALYQAHLAMGYAPISIPAPMRNDDLVSIAATYDRAVIAYLGKDVPGGGSTRITRHDAGAGSV
VSFDYNAAVQASAVPRLGQVYVLISNDGQGARAVLLAEDLAWAGSGSALDVLNERLVTLFPAPV
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A dnt mutant in Bordetella Bronchiseptica was attenuated and provides protection in guinea pigs against intranasal challenge with strain L3 (Nagano et al., 1988).
  • Related Vaccine(s): Bordetella bronchiseptica dnt mutant vaccine
5. trpE
  • Gene Name : trpE
  • Sequence Strain (Species/Organism) : Bordetella bronchiseptica
  • NCBI Protein GI : 8515760
  • Other Database IDs : CDD:184146
    CDD:147064
    CDD:187007
  • Taxonomy ID : 518
  • Gene Strand (Orientation) : ?
  • Protein Name : anthranilate synthase large subunit
  • Protein Length : 506
  • Protein Note : anthranilate synthase component I; Provisional
  • Protein Sequence : Show Sequence 
    >gi|8515760|gb|AAF76162.1|AF266751_1 anthranilate synthase large subunit [Bordetella bronchiseptica]
MTELEFKALAAQGYNRIPLVAETYADLDTPLAIYLKLAHSGPQGGRMSCLMESVVGGERFGRYSFIGLPA
RTVIRASGSTTEVLQDGHVAETHEGDPLAFIESYQARFKVALRPGMPRFCGGLAGYFGYDTVRHIEQRLG
PAVKPFPDGMEEGTPDIMLLHVDELVIVDNLAGRTYLMVYADPAQPEAYSRAQQRLLELRARLRRPVEIP
YSHASMQTEERRDFQKADYLAAVARAKEYIAAGDVMQVQVGQVIAKPFRDAPLSLYRALRSLNPSPYMYF
WNFGDFQVVGASPEILVRQEQIDDQGETRSQITIRPLAGTRKRGGTPEEDLALAAELQADPKERAEHVML
IDLARNDVGRVAEIGSVRVSDTMVIERYSHVMHLVSNVAGKLLPGMSSMDVLRAAFPAGTLTGAPKVRAM
EIIDELEPVRRGIYGGAAGYLSYGGEMDVAIAIRTGVIKNGTLYVQAAAGIVADSSPEAEWAETEAKARA
VLRAAEQVQHGLDEPI
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A trpE mutant, in combination with an aroA mutation, is attenuated in mice and induces significant protection from challenge with wild type B. bronchiseptica (McArthur et al., 2003).
  • Related Vaccine(s): Bordetella bronchiseptica aroA/trpE mutant vaccine
1. Ifng (Interferon gamma)
  • Gene Name : Ifng (Interferon gamma)
  • Sequence Strain (Species/Organism) : Mouse
  • NCBI Gene ID : 15978
  • NCBI Protein GI : 33468859
  • Genbank Accession : NM_008337
  • Protein Accession : NP_032363.1
  • Other Database IDs : MGI:107656; UniProt: P01580
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence 
    >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
AGTATACCTTTGCAACC
  • Protein Sequence : Show Sequence 
    >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
LLPESSLRKRKRSRC
  • Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
  • Additional Molecule Role : Vaximmutor
  • Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
  • Related Vaccine(s): Bordetella bronchiseptica bscN and cyaA double mutant vaccine
2. IgA
  • Gene Name : IgA
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 238447
  • Genbank Accession : AC160982
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 8607
  • Gene Ending Position : 12639
  • Gene Strand (Orientation) : -
  • Protein Name : immunoglobulin heavy constant alpha
  • Protein Note : Also known as IgA; Igh-2
  • DNA Sequence : Show Sequence 
    >gi|121699722:8607-12639 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
AGTACTGGGGGACCTCTTTGCTGCCAAACGGGCCTCGAACAGTTGTAACAGTGAGTGCTGTGCTGTAGAA
CAAGCTCAGTAGGAAGAGGGTGAGGAAGGTCACAGTGGTGGGCCACAGGCTGGCACCTGGGGCCTCTTCC
TCCAGGATGTCTTCTGACTGGTCCAGTAGCACATAGGAAAGTGGCTCTTGACGTTCTGTAAGACATAGAT
GACCTTCTAGATCCAAACCCATGGGGATGGGGGCTCAGACTTAAGGTTTCTGCTTCTATGCCTCCTTGGG
CTTTGGGGACAGTGGTGGTAGGGGTCCCTTATTCCCGGGAAGGGACTTGTTCAGGGATAATGAGGAGGCA
GGGTCCCAGGTAAGGGACTGGAATCCCAGCAGTTCTTGAGATGGCCCCAAGACCCATCTCTCTCTCTTAG
GGAAACCTTAATTAGAGACCTGTCATGGTAAGAGAGTTGTTTCTGTCAAACTCTGAGAAGAGTTTGAACC
CAAGGCTCCTTAGGTCTCAGTCCTTTCTAGATATTTAGCAATACCTCCCTAAACCCCAGTTCTTAGGCTA
ATCTTGGCTTGGGTACTAGGGTTGTGTAAGATCAGACACCTTCAAACAGATACAGGCCAATGTGATTTCT
GGGCCAAAGTTAGACTACCAAGGCCTGGACATGTGGTCAGAGGACTCTGTTGCATTCTTAAGAGCTGTGA
CTGGGGGTTAAGCAACAGTAAAGACCCCCAGTAGAGCCAACCCTGCTGTCCTCAGGTTCTCTGCGCACAG
AGTTTCATTTTAAGTGGCAGTAGGAAATGTGTACTGATAGTCAACCACTCGTATTTCTGTCACTGCAGCC
ATCCATCAATCCATCCATCATCCCCATCGTTCATCCTTGTAGCCAGCCATCCATTACCCTCCAGCCGCTC
ACTCATCATCTTTCCCGACTATCGTGATTCTCCAGCTTCTCTGCCCCCTCTCCCTGTGGGGATTCAGTCT
TTCACCTATTGACTCCTCCTTACTAGGATTCTCTGTCATGGGCCAGGCCTTGGATTCCATGGGTGGCTAT
GGTACTTTTCTGAGTCAGGTTTCCCAAGCTGGTCTGTTGCTCTTGCTATTCTCAGGGAACTGGACTTTCA
AGCTGGAGAGTATCCAGGGCATGCATGGGCCTTTACTCCACTCTTGCTCTGAGAGCAGAGGGAAGTTGCT
AGAAACCAGCAACTCCTCCCCCCCCCCCCCGAAGTTCTGAAGGCCTGTGAGACAGGAGCAGGGCCCCCAC
TGCCCTCTTGTGGACACCTGGGCTCCAAGTATACCTGGTTGTAGAACTTTGTGTTCTAGGGGTGCCCATG
GAGGAGGCCGAGGCCTGTGTGGGAGTGGTCTCCATGGCCTGCTCCTGCTTCTTCCCAGATTTGCCTCTTA
TCCTGCTTCTGGTTTGTGATACTTGGGCTCAGGATGGGAAGGGAGACTTAGGTTAGAAAGGGGGCACCTG
GAGGGGCCTATGCCCTTGTCCATTGTGTGTAGTTGTCAGTGTCGGGCCTGGAAATTCTCCCGGGGGCTTG
GAGGGGACTCCCCTTGCTTTCCAGAAGCTGGGTGTGGGCTGTGAACTTCATCTTCCTCCAGGGTTCAGGT
CTGTGTGAGTGTGCAAATGTGAGTGTCTGTGCATGTATGCCCATGTGCATGTATGCAGAACAGGGTGTAT
GCCCATGTGCATGTGTGAAGAGCAGGGTGCTGCTGAGGGTTGTCTGTGCCATGCTATATGTCTTTCTCAT
GAGTCAGTGGTCACCCTGAATGCATGTTTCCATTCAGGGATGGCCTACTGAGGGTGCGTGGCATCTTCTT
CCCAGTGCCCCTGTGTTCATCCCTTCATTCTCATACCATCATCTCTATTGTGACCCCCACACGGCACCAG
CCTTGATGGTGCTACCCTTTTGCTCCATCTATCCCTGTCTTGTTTGGGTTAGAAGCTGCGGTCTAATAAA
CAGCCACACCACCCACCACTCAGAGTACTTGTGCAGCAACCCACTGCCCTGGGGACTCTCCCATGAGCTT
TGCCTTCTTGGCTGAGTCTGCTCGCCTTGGATTTCATCCCCAGTTTATTCTTACTTCCCATTCACTCATT
GCACATTCACAGGGCAGGTGCACACACTCTCATGTTTCCACCAACATGAACACACATAGATATATGCATG
CTCATAGGTATGCACAGGCACACACTTGCTCACAGGGACATAGATATAGTGTGTCCACATATGTACTTAT
ACCCATATACATACCTATAAACCATAGGTGCACACAAACTCACTCCTCTCTTGCTGCACACAGGTACACA
CATGAAGGCACATGGACCCATCCATAAGCACTCACCAATACATAGACATGCATACACATATACTCTAGGC
ATGAAGGGTCTTCAAAGTCTCCGAGGCCTTACAAGCTTAGGTGGTGGTGTTTCTCCCTCCCTGGGCCCTG
ACCCCTCCCTGTGTTCTGTAGACCCTAAGGATGATGGACAGGCACTGGATGGAAGTGCAGGGATACTTTG
GATGAGCACAGAGTTTATTTCAGGAGTAGGGACAGGCAGGGTGGCTCAGTAGCAGATGCCATCTCCCTCT
GACATGATCACAGACACGCTGACATTGGTGGGTTTACCCGACAGACGGTCGATGGTCTTCTGGGTGAAGT
TCATGGGCAAGGCCTCGTGGCCCACCATGCAGGAGTACTGGTCACCCTGTTTCCAGAGTTCAGCTGATAC
ACGCAACACGCTTGTCACCAGGTAGGTGGTGGCTCCCTCGCCTGGCTCCTTTAGGGGCTCAAACACTAGG
TAGCTTTCTGGGGACAGCTCCTCATTTCCATGCAGCCATCGCACCAGCACTTCTTTAGGGTTGAAAGCTC
GCACCAGGCATGTCAGGGACACGAGCTCATTCAGGGCCAGCTCCTCCGACGGCGGCGGTAGCAGGTGGAC
CTGGGGTGGGAAGGTGTTCACTGGAAGGTAGAAAGAAGAGTTATGGCTCAGACAAGGAGAGCAAGACCCC
TCTGCCCTTCCCTTCTGGGCAGTTCCTCAGATAGCTCCAATTTCCCTCTGTGATATAAGGACAGGAGCAG
TTAGTTGAGCATCTGTATTATAGGAAGAAAGCAGGCATGTGAGCAGGGAACGTCATACAATGTCCCGGGT
ATGCATCTGGGCTCACCTGTGATTTTGGCAATTGTGCCAGTTAAGGTGTCAGACTCAGGATGGGTAACTG
TGCACTTGAATGATGCGCCACTGTTCCAGCGCTCAGCACAGCCAGGCAGGACGCTGGACACACTGTAGCA
GCCGCAGGAATTCTGCACAGCTTTCTTCTGCACTGCATCCTTCCCAGTGGAGGGCTCCCAGGTGAAGACA
GCTCCCTCAGGATTTCTCAGGCCATTCAGAGTACATGTGAGGCTGGCATCTGAACCCAGGAGCAGGTCCT
CAAGAGCTGGCCGCTGCAGTGACAGGCTGGGATGGCAGGAAGGAGGACAAGGAGGACAAGGAGGAGGAGG
ACCTGTAACAGAGAAGTCCTAGCAAATCAGTATATTCTCCTTTTCTCCTTTGCTGTCCCCTTTTCTGGAT
GTTCTGAGGCCTCAGTTCCTTATGGCCTGTTTACGCTTCCCTTCCCCCACCCCCATGGGAGGTTTCTGAG
GGTCCCTGTGAGGGTGTGCCTCAGAGGGATATATGGATCTAGATAAGGTAGAACTTATCCCACCCCCAGC
TGACCCCCTAACGTTCTTTACCAGAGCACTTCACATCCAATTCTTGGACGGCGTTAGAGTCATGTTGCAC
GGAACATTTCACGGATTCTCCTTCTGGGCACTCGACAGCTGGCAGGGTCAACTGGCTGCTCATGGTGTAC
CCTCCCCCAGAGGCCAGGGCAGGTGGGAAGTTTACGGTGGTTATATCCTTCCCACTCTTTCCCCAGGTCA
CATTCATCGTGCCGGAAGGGAAGTAATCGTGAATCAGGCAGCCGATTATCACTGGGTCACTTGACAGAGC
TCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGAC
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bordetella bronchiseptica aroA mutant vaccine (strain BBS18)
3. IgG
  • Gene Name : IgG
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16059
  • Genbank Accession : AF010213
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 881411
  • Gene Ending Position : 914690
  • Gene Strand (Orientation) : +
  • Protein Name : immunoglobulin heavy chain (V7183 family)
  • Protein Note : Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)
  • DNA Sequence : Show Sequence 
    >gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
ACAAGCACAGTGCAAATTTCTGTCACCCTG
  • Molecule Role : Vaximmutor
  • Additional Molecule Role : Vaximmutor
  • Related Vaccine(s): Bordetella bronchiseptica aroA mutant vaccine (strain BBS18)
4. IgM
  • Gene Name : IgM
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16019
  • Genbank Accession : AC073553
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 171229
  • Gene Ending Position : 175133
  • Gene Strand (Orientation) : -
  • Protein Name : immunoglobulin heavy constant mu
  • Protein Note : Also known as Igm; muH; Igh6; Igh-6; Igh-M; AI326478; MGC107036; MGC107507
  • DNA Sequence : Show Sequence 
    >gi|121699722:171229-175133 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
GTCATTTCACCTGCAGGTGACAGAGACAGTGTGAATTGCTGTCCACTTGTAGGGAGTAGAGGTAGAGGCA
GTGAGGACTGGTGACTCCCTGTCCCAGCCCTGTGTCCTCAGCCCCACAACCACACTACCTTGAACAGGGT
GACGGTGGTGCTGTAGAAGAGGCTCAGGAGGAAGAGGACGATGAAGGTGGAGGCAGTGGTCCACAGGTTC
TCAAAGCCTTCCTCCTCAGCATTCACCTCCCCCTCTATGAAGGGACACAGAGAGGGAGGGTCAAGCCAAG
TCTTGGGGTGACTGAGCATTTGCATAAGGGTTGGGGTGCCCTGATGGTTCTGGAGAATCTTAGCGAGTAT
TGACTGGTCCCCTAATAGCTCAAGCTAGACGGAGGCACAGAACAGGCCAAGCTGTAGGACACACTAATTT
GGAGCCTTTGTTCCAGGAGCCCTGGGGCAGCAGAGCAAGCAAGACAGATGCAATGTATGGACCTGGGTAA
AGGCAGACTCAGGAACTGTGGCAAAATGATATTGGTGTGCTCTGGACTTTCCCTGAGCAGGTGGTGCCAT
CGGTGCTGTTTAGACTGAGAATTTAGAGCAGGTCTCCCTGTCTCTTGGGCTTTAGCAATAAGAACAGGGA
TACCAGAGGTTGTCCCTCCTTACTTCCTGAAGGACTCAGGCATCTCATGACTTTCTTTGATCAGAGTTCC
CGTGCGTTGCTTGTCCTACCCCTGCCAGCAATGTTGGGTCTTGGTACCAAGAGATAAACAGACAGATACC
TGAGGGAGGCTGTGGGACCAGGGCATTTATTGGGATCTCTGATCCCTGTAAAGATGAGCCCTTGGGGATA
TCTAAAGCCTAGGCCCCAAATGGATATGAATATGAAGAATGTCCAGGATATGAGCCCAAAGAGGCAGTGT
TATTGGGTGGTGACTCCTTACAGAGGCTCCTTGATGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
GTGTGTGTGTGTGTGTGTGTGTGTGTGGTTGGGGCATCTTTTGGACAACTGCATTATGTTTTCTGCATTT
TGAACTGGAGATCAGGGTGGGTGTGAGTGGGTTAGGCAATTTCACATTGGTCTTCTGAGGTGTTCTATTA
ATGGAGACACAGCCAAGGCTAACTTTCTGTCATGGGTGGTTAGTGGAGTATCTTCTGCCCCATGGTGTTG
AGACAGATAGGCATACTTTGTACCTGGGGTTATCTCTCCCAATCATAGAGTGGGCTCCTGAGGAGTATCA
GTGACCCAGCCTAGTGTAGGCTCTCTGAGCCGTGAGCCTTGGAGATTACAGCCCTGGTCAGAGTCCAGGT
GTATCTGTGTGCTATACCTGTTCACATGTTAGTGTACACATGTGAAGATGTTTCTGTACATGTGTGTATA
TGCATATCTTTGTATCTATATAGTTTATTTGTGTATATTTGCCTATAGTCTGTTTCTGTGTACACAGGAC
CATGCATATTGGTATCTGTTTCTGTGAGTGGTTGTGTGTCTATGCCTATTTGTCTCTGAAGGCGTTTGTA
AGTGTCCATGCATGTGTGTGCGCATGAACTCATGTGTATATATGTGTACCTTTGTCTGTGTGTCCATGAA
TACACATTCATGTATGTGTCTACACATGTGTACCTATATATCTATGTATAAATGCATGTGTGTCTATATA
TGTGCCTGAATGCTGCCTGCACCAGGTTAGGCAAGCTTTCAGCCATATATCTAGAAGTAGCTGGAGGAAT
CGCATGTTCATACTCTGTCTGTTGTAAGCCTGACTGAGTTCACACACAAGGAGGAAACTGGCAAGGTAGA
GGAAGGGCCAGGGTGGAGGCAAGTATGCAGGGTGTGTGCAGAGCAAGCAAAACACAACTGTCTCAGCAGG
CATGAGCATTGTATAATCAAAACCAGTGACGTTTGAATGGATTTTTTTTATTTCTAATTTTTATAAAATG
CAACATCTCACTCTGACATGGTTAGTTTGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGA
GCGCTAGCATGGTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTACAGTGTGGGT
TTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGTGGCAGGGCCTCGTGGCCTACAACACAGG
TATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGCTGTGGGTAAAGTAGAAGCCTGG
GGCCCCAGGCTCTGGCATCGGGGCACTGGTCACATACTTCTCTTGGGGCAAGAGTTGCCCTCTCTGAAGC
CACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAGGCAGGTGACTGTGGCTGACTCCCTCAGGT
TCAGTTGCTCACGAGCTGGTGGCAGCAGGTACACAGCAGGTGGATGTTTGTGCACCTCTGTAAATGAAGA
CAGTAGTGAGGATAGGGTGGAAGAGGACCTGCCCTCCCTATGAGGTACAGGAAGGGTCCTGCAATTGGAG
GGGAAGGGAAGGGGGGATACCTACCATTGGGTTTTGAGATGAATTTCTTCTGTGGTGAAGGCAGATCCCT
GTGAGTCACAGTACACACAAATTCCTTCCTGTTATTCCAGTCTTCCACACAAACACTAGCCACACCCTTA
GCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTTGGTTTCCAGTGGTTCACCACTTTGAG
AAGCCCAGGAGATATTCAGGGTTTCATAGGTTGCCAGGTTTGAGACCAGACAGGTCAGGTTAGCGGACTT
GCTGAGGAAGATGTCGGCAAAGGAGGGGGGGATGGTGAAGGTTAGGATGTCTGTGGAGGGACCTGCAGTC
AAGAGAACACTGTGTCAGGTTATGACATATCTAGTTGTGAAGTTGGAAAAAAGCTTAGTCTCCATCCCCC
AAACCTACTTAAGGGAAGATGCTGGTCCTTCCAGCTGATTCTCACCCCTCTACTGCCTATTGCTCATTCT
TGTCCTTGGCCACTCTGGGGAGCCAAAGTTCAAGGAGCAAATGACCATGTCTGGGTGGCATTGGCCATAA
TTGTAGGAGGACTTCCCTAATCTGGGAGGGCTAGGCATTGGGCTTAGCCCAGGCCACTCACTGGCAGCAC
ATGTGGAGGACACGTTCTTCAAGAAGGTGAGACCCCTGTGATCCACACGGCAGGTGTACACATTCAGGTT
CAGCCAGTCGATTTCAGAGATGGTAAGTGTGCTTATGACCTTGTAGGTTTGGGGTGTGGATCCTTTGTTC
TCGATGGTCACCGGATCTGTGGTGAAGCCAGATTCCACGAGCTTCCCATCCTTTAGCCAGGATACTGTGA
TCGGTTTTGGAGTGAAGTTCGTGGCCTCGCAGATGAGTTTAGACTTGCGTGGTGCAGGGCCAGAGAAGCC
ATCCCGTGGTGGGACGAACACATTTACATTGGGGTTCATCTCTGCGACAGCTGGAATGAAAGGTCAAGGT
GTGAGCTCAGCTTGGCCTAAGCCCCACCCCGCTGCTCCCTCTGGGCCATGCCTGGGCCTGGGCACCCCTG
CTGGGAGGGTTTGGTTCTTACCTGGAATGGGCACATGCAGATCTTTGTTTTTGCCTCCGTAGTGGATTTT
GCATACCAGGTATTCATCTGAACCTTCAAGGATGCTCTTGGGAGACAGCAACACCTGCGAGGTGGCTAGG
TACTTGCCCCCTGTCCTCAGTGTTGGGAAGGTTCTGATACCCTGGATGACTTCAGTGTTGTTCTGGTAGT
TCCAGGTGAAGGAAATGGTGCTGGGCAGGAAGTCCCGGGCCAGGCAGCCCATGGCCACCAGATTCTTATC
AGACAGGGGGCTCTCGCAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTC
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bordetella bronchiseptica aroA mutant vaccine (strain BBS18)
5. Il10 (interleukin 10)
  • Gene Name : Il10 (interleukin 10)
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16153
  • NCBI Protein GI : 6754318
  • Locus Tag : AL513351.1
  • Genbank Accession : NM_010548
  • Protein Accession : NM_010548
  • Other Database IDs : MGI:96537; Ensembl:ENSMUSG00000016529;
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence 
    >gi|6754317|ref|NM_010548.1| Mus musculus interleukin 10 (Il10), mRNA
GGGGGGGGGGATTTAGAGACTTGCTCTTGCACTACCAAAGCCACAAAGCAGCCTTGCAGAAAAGAGAGCT
CCATCATGCCTGGCTCAGCACTGCTATGCTGCCTGCTCTTACTGACTGGCATGAGGATCAGCAGGGGCCA
GTACAGCCGGGAAGACAATAACTGCACCCACTTCCCAGTCGGCCAGAGCCACATGCTCCTAGAGCTGCGG
ACTGCCTTCAGCCAGGTGAAGACTTTCTTTCAAACAAAGGACCAGCTGGACAACATACTGCTAACCGACT
CCTTAATGCAGGACTTTAAGGGTTACTTGGGTTGCCAAGCCTTATCGGAAATGATCCAGTTTTACCTGGT
AGAAGTGATGCCCCAGGCAGAGAAGCATGGCCCAGAAATCAAGGAGCATTTGAATTCCCTGGGTGAGAAG
CTGAAGACCCTCAGGATGCGGCTGAGGCGCTGTCATCGATTTCTCCCCTGTGAAAATAAGAGCAAGGCAG
TGGAGCAGGTGAAGAGTGATTTTAATAAGCTCCAAGACCAAGGTGTCTACAAGGCCATGAATGAATTTGA
CATCTTCATCAACTGCATAGAAGCATACATGATGATCAAAATGAAAAGCTAAAACACCTGCAGTGTGTAT
TGAGTCTGCTGGACTCCAGGACCTAGACAGAGCTCTCTAAATCTGATCCAGGGATCTTAGCTAACGGAAA
CAACTCCTTGGAAAACCTCGTTTGTACCTCTCTCCGAAATATTTATTACCTCTGATACCTCAGTTCCCAT
TCTATTTATTCACTGAGCTTCTCTGTGAACTATTTAGAAAGAAGCCCAATATTATAATTTTACAGTATTT
ATTATTTTTAACCTGTGTTTAAGCTGTTTCCATTGGGGACACTTTATAGTATTTAAAGGGAGATTATATT
ATATGATGGGAGGGGTTCTTCCTTGGGAAGCAATTGAAGCTTCTATTCTAAGGCTGGCCACACTTGAGAG
CTGCAGGGCCCTTTGCTATGGTGTCCTTTCAATTGCTCTCATCCCTGAGTTCAGAGCTCCTAAGAGAGTT
GTGAAGAAACTCATGGGTCTTGGGAAGAGAAACCAGGGAGATCCTTTGATGATCATTCCTGCAGCAGCTC
AGAGGGTTCCCCTACTGTCATCCCCCAGCCGCTTCATCCCTGAAAACTGTGGCCAGTTTGTTATTTATAA
CCACCTAAAATTAGTTCTAATAGAACTCATTTTTAACTAGAAGTAATGCAATTCCTCTGGGAATGGTGTA
TTGTTTGTCTGCCTTTGTAGCAGCATCTAATTTTGAATAAATGGATCTTATTCG
  • Protein Sequence : Show Sequence 
    >gi|6754318|ref|NP_034678.1| interleukin 10 [Mus musculus]
MPGSALLCCLLLLTGMRISRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSL
MQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVE
QVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS
  • Molecule Role Annotation : IL-10 plays an important role in Th2 type immune response.
  • Related Vaccine(s): Bordetella bronchiseptica bscN and cyaA double mutant vaccine
IV. Vaccine Information
1. Bordetella Bronchiseptica Avirulent Live Culture Vaccine (USDA: 1081.00)
a. Manufacturer:
Wyeth, MVP Laboratories, Inc., Addison Biological Laboratory, Inc.
b. Vaccine Ontology ID:
VO_0001741
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Gray wolf
2. Bordetella Bronchiseptica Avirulent Live Culture Vaccine (USDA: 1081.01)
a. Manufacturer:
Intervet Inc., Novartis Animal Health US, Inc.
b. Vaccine Ontology ID:
VO_0001742
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Gray wolf
3. Bordetella bronchiseptica aroA mutant vaccine (strain BBS18)
a. Vaccine Ontology ID:
VO_0002801
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Gene Engineering of aroA
  • Type: Gene mutation
  • Description:
  • Detailed Gene Information: Click here.
e. Preparation
A B. bronchiseptica aroA mutant was constructed by allelic exchange. Introduction of the Km-R cassette into the aroA gene of this strain was confirmed by PCR using aroA-specific primers (aroAfw 5′GGCGTGCAAAGCGGGGCGGACTGGCTGGAG3′ and aroArv 5′ATAATCGGGGAAAGTCTTGCTGACGCAGCC3′) and by Southern blotting and DNA:DNA hybridization using an aroA-specific probe generated by PCR (Stevenson and Roberts, 2002).
f. Immunization Route
intranasal immunization
g. Mouse Response
  • Host Strain: BALB/c
  • Persistence: Wild type B. bronchiseptica could still be detected in lungs up to 56 days post-infection. In contrast, the numbers of BBC18 did not increase following inoculation, but decreased rapidly, and were cleared from the lungs completely by day 6.The nasal cavity samples showed the same trend and were also clear of bacteria after 6 days. The behaviour of the mutant and the wild type strain was mirrored in outbred NIH-S mice. Thus the B. bronchiseptica aroA mutant is highly attenuated (Stevenson and Roberts, 2002).
  • Efficacy: Immunization with GVB120 (B. bronchiseptica aroA mutant expressing FrgC), by either regime, had a major effect on colonization of the lungs, trachea and nasal cavity by wild type B. bronchiseptica BBC17. The effect of immunization on the subsequent colonization with BBC17 was more pronounced in the lower than the upper respiratory tract (Stevenson and Roberts, 2002).
  • Host Gene Response of IgA
    • Gene Response: High titers of anti-B. bronchiseptica IgA were detected in serum samples from immunized, but not naive, mice prior to challenge (Stevenson and Roberts, 2002).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: High titers of anti-B. bronchiseptica IgG were detected in serum samples from immunized, but not naive, mice prior to challenge (Stevenson and Roberts, 2002).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgM
    • Gene Response: High titers of anti-B. bronchiseptica IgM were detected in serum samples from immunized, but not naive, mice prior to challenge (Stevenson and Roberts, 2002).
    • Detailed Gene Information: Click here.
4. Bordetella bronchiseptica aroA/trpE mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of aroA
e. Gene Engineering of trpE
f. Immunization Route
intranasal immunization
g. Mouse Response
  • Persistence: An aroA/trpE mutant is attenuated in mice (McArthur et al., 2003).
  • Efficacy: An aroA/trpE mutant induces significant protection from challenge with wild type B. bronchiseptica (McArthur et al., 2003).
5. Bordetella bronchiseptica bscN and cyaA double mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Gene Engineering of bscN
  • Type: Gene mutation
  • Description:
  • Detailed Gene Information: Click here.
d. Gene Engineering of cyaA
  • Type: Gene mutation
  • Description:
  • Detailed Gene Information: Click here.
e. Preparation
An isogenic mutant of RB50 containing an in-frame deletion of the adenylate cyclase toxin gene (cyaA), was constructed with an in-frame deletion of the ATPase (bscN) gene required for type III secretion (Mann et al., 2007).
f. Immunization Route
intranasal immunization
g. Mouse Response
  • Persistence: TLR4def and TNF-α−/− mice were intranasally inoculated with 103, 104, or 105 CFU of RB50 or 105 CFU of AVS in a 50-μl inoculum and observed them for signs of severe disease. WT mice given similar doses of RB50 are able to control the disease and to clear bacteria from the lower respiratory tract (Mann et al., 2007).
  • Efficacy: The nasal cavities of vaccinated TLR4def mice and TNF-α−/− mice contained 10,000-fold fewer CFU than those of control mice. The tracheae and lungs of vaccinated TLR4def mice and TNF-α−/− mice contained <20 CFU, while the same organs of control mice contained approximately 106 and 108 CFU, respectively. These results indicate that low-dose intranasal vaccination with AVS protects susceptible mice from severe infection by a variety of B. bronchiseptica strains (Mann et al., 2007).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: AVS induces significantly increased IFN-gamma production in mouse splenocytes as compared to naive mice. This increase occurred 28 days after vaccination and after stimulation with heat-killed RB50 (Mann et al., 2007).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Il10 (interleukin 10)
    • Gene Response: AVS induces decreased IL-10 production in mouse splenocytes as compared to inoculation with wild type strain. This decrease occurred 28 days after vaccination and after stimulation with heat-killed RB50. However, IL-10 was significantly upregulated in splenocytes compared to naive mice (Mann et al., 2007).
    • Detailed Gene Information: Click here.
6. Bordetella bronchiseptica dnt mutant vaccine
a. Vaccine Ontology ID:
VO_0002805
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Gene Engineering of dnt
  • Type: Gene mutation
  • Description:
  • Detailed Gene Information: Click here.
e. Preparation
Dom+ Scs+ Hly+ organisms of strain L3 were streaked on two BG-20 agar plates and incubated at 37°C. Ten Dom+ Scs+ Hly- colonies (0.5 to 0.7mm in diameter) grown on each plate were subcultured 10 times under the same culture conditions. Finally, four mutant strains showing the Dom+ Scs+ Hly -phenotype and lacking DNT- producing ability were obtained (Nagano et al., 1988).
f. Immunization Route
intranasal immunization
g. Guinea pig Response
  • Efficacy: Most of the guinea pigs inoculated intranasally or intramuscularly with 2.3 x 10^7 or 2.3 x 10^8 viable cells of strain B-42 produced serum agglutination antibodies at 3 week after the inoculation, and most of them survived intranasal challenge with strain L3 (770 x LD50) during observation period (Nagano et al., 1988).
7. Canine Adenovirus Type 2-Parainfluenza-Bordetella Bronchiseptica Modified Live Virus & Avirulent Live Culture Vaccine (USDA: 12X1.20)
a. Manufacturer:
Wyeth, Intervet Inc.
b. Vaccine Ontology ID:
VO_0002089
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Gray wolf
8. Canine Parainfluenza-Bordetella Bronchiseptica Modified Live Virus, Avirulent Live Culture Vaccine (USDA: 14M1.20)
a. Manufacturer:
Boehringer Ingelheim Vetmedica, Inc., Intervet Inc.
b. Vaccine Ontology ID:
VO_0002119
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Gray wolf
9. Canine Parainfluenza-Bordetella Bronchiseptica Modified Live Virus, Avirulent Live Culture Vaccine (USDA: 14M1.22)
a. Manufacturer:
Intervet Inc.
b. Vaccine Ontology ID:
VO_0002120
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Gray wolf
10. Porcine Rotavirus-Transmissible Gastroenteritis Modified Live Virus Vaccine-Bordetella Bronchiseptica-Clostridium Perfringens Type C-Erysipelothrix Rhusiopathiae-Escherichia Coli-Pasteurella Multocida Bacterin-Toxoid (USDA: 49T9.21)
a. Manufacturer:
Intervet Inc.
b. Vaccine Ontology ID:
VO_0002319
c. Type:
Live, attenuated vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Pig
V. References
1. Goodnow, 1980: Goodnow RA. Biology of Bordetella bronchiseptica. Microbiological reviews. 1980; 44(4); 722-738. [PubMed: 7010115].
2. Mann et al., 2007: Mann P, Goebel E, Barbarich J, Pilione M, Kennett M, Harvill E. Use of a genetically defined double mutant strain of Bordetella bronchiseptica lacking adenylate cyclase and type III secretion as a live vaccine. Infection and immunity. 2007; 75(7); 3665-3672. [PubMed: 17452472].
3. Mattoo et al., 2001: Mattoo S, Foreman-Wykert AK, Cotter PA, Miller JF. Mechanisms of Bordetella pathogenesis. Frontiers in bioscience : a journal and virtual library. 2001; 6; E168-186. [PubMed: 11689354].
4. McArthur et al., 2003: McArthur JD, West NP, Cole JN, Jungnitz H, Guzmán CA, Chin J, Lehrbach PR, Djordjevic SP, Walker MJ. An aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica is attenuated and immunogenic in a mouse model of infection. FEMS microbiology letters. 2003; 221(1); 7-16. [PubMed: 12694904].
5. Nagano et al., 1988: Nagano H, Nakai T, Horiguchi Y, Kume K. Isolation and characterization of mutant strains of Bordetella bronchiseptica lacking dermonecrotic toxin-producing ability. Journal of clinical microbiology. 1988; 26(10); 1983-1987. [PubMed: 3182989].
6. Scheiblhofer et al., 2003: Scheiblhofer S, Weiss R, Dürnberger H, Mostböck S, Breitenbach M, Livey I, Thalhamer J. A DNA vaccine encoding the outer surface protein C from Borrelia burgdorferi is able to induce protective immune responses. Microbes and infection / Institut Pasteur. 2003; 5(11); 939-946. [PubMed: 12941385].
7. Stevenson and Roberts, 2002: Stevenson A, Roberts M. Use of a rationally attenuated Bordetella bronchiseptica as a live mucosal vaccine and vector for heterologous antigens. Vaccine. 2002; 20(17-18); 2325-2335. [PubMed: 12009288].