VIOLIN: Vaccine Investigation and Online Information Network

Pasteurella multocida

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. OmpA (Protective antigen)
2. ompH (Protective antigen)
3. ompH (Protective antigen)
4. aroA (Virmugen)
Vaccine Related Host Genes
1. IgG
2. IgM
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

747

2. Disease:

Pasteurellosis, fowl cholera

3. Introduction

Pasteurella multocida is a Gram-negative, non-motile coccobacillus that is penicillin-sensitive and belongs to the Pasteurellaceae family. It can cause a zoonotic infection in humans, which typically is a result of bites or scratches from domestic pets. Many mammals and fowl harbor it as part of their normal respiratory microbiota, displaying asymptomatic colonization. P. multocida is the most common cause of infection from animal injuries (pneumonia in cattle and pigs, atrophic rhinitis in pigs and goats, and wound infections after dog/cat-bites.) A high leukocyte and neutrophil count is typically observed, leading to an inflammatory reaction at the infection site (generally a diffuse localized cellulitis). It can also infect other locales, such as the respiratory tract. In more serious cases, a bacteremia can result, causing an osteomyelitis or endocarditis. The bacteria may also cross the blood-brain barrier and cause meningitis (Wiki: P. multocida).

4. Microbial Pathogenesis

In birds, P. multocida is believed to enter through the respiratory tract tissues of the host. P. multocida has been shown to adhere to air sac macrophages. The mode of transfer from the lungs to the bloodstream is poorly understood, but heterophils may play a role in the process (Harper et al., 2006).

5. Host Ranges and Animal Models

P. multocida causes can cause disease in fowl, pigs, cattle, rabbits and humans (Harper et al., 2006).

6. Host Protective Immunity

The first systemic host defence used against most invading bacteria involves the innate immune system and includes phagocytosis and bactericidal properties of serum components such as complement. However, P. multocida has mechanisms to evade this system, including the presence of a capsule. Active immunity against P. multocida infection is mainly humoral; vaccination of birds with killed P. multocida stimulates protection against homologous challenge, and opsonization studies in mice have shown that bactericidal antibodies are produced (Harper et al., 2006).

II. Vaccine Related Pathogen Genes

1. aroA

Gene Name : aroA
NCBI Protein GI : 581456
Other Database IDs : CDD:179423
CDD:30129
GOA:Q04570
InterPro: IPR001986
InterPro: IPR006264
InterPro: IPR013792
InterPro: IPR023193
UniProtKB/UniProt: Q04570
Taxonomy ID : 747
Gene Strand (Orientation) : ?
Protein Length : 441
Protein Note : 3-phosphoshikimate 1-carboxyvinyltransferase; Provisional

Protein Sequence : Show Sequence

>gi|581456|emb|CAA78480.1| aroA [Pasteurella multocida]
MIKDATAITLNPISYIEGEVRLPGSKSLSNRALLLSALAKGKTTLTNLLDSDDVRHMLNALKELGVTYQL
SEDKSVCEIEGLGRAFEWQSGLALFLGNAGTAMRPLTAALCLSTPNREGKNEIVLTGEPRMKERPIQHLV
DALCQAGAEIQYLEQEGYPPIAIRNTGLKGGRIQIDGSVSSQFLTALLMAAPMAEADTEIEIIGELVSKP
YIDITLKMMQTFGVEVENQAYQRFLVKGHQQYQSPHRFLVEGDASSASYFLAAAAIKGKVKVTGVGKNSI
QGDRLFADVLEKMGAHITWGDDFIQVEKGNLKGIDMDMNHIPDAAMTIATTALFAEGETVIRNIYNWRVK
ETDRLTAMATELRKVGAEVEEGEDFIRIQPLNLAQFQHAELNIHDHRMAMCFALIALSKTSVTILDPSCT
AKTFPTFLILFTLNTREVAYR

Molecule Role : Virmugen
Molecule Role Annotation : An aroA mutant is highly attenuated in calves and induces significant protection from challenge with wild type P. multocida (Dagleish et al., 2007).
Related Vaccine(s): Pasteurella multocida aroA mutant vaccine

2. OmpA

Gene Name : OmpA
Sequence Strain (Species/Organism) : Pasteurella multocida strain 95010872
NCBI Nucleotide GI : 49182347
NCBI Protein GI : 49182348
Protein Accession : AAT57676.1
Other Database IDs : CDD:236764
CDD:279698
CDD:143586
Taxonomy ID : 747
Gene Strand (Orientation) : ?
Protein Name : OmpA
Protein pI : 9.2
Protein Weight : 34678.17
Protein Length : 396
Protein Note : outer membrane protein A; Reviewed

DNA Sequence : Show Sequence

>gi|49182347|gb|AY643794.1| Pasteurella multocida strain 95010872 OmpA gene, complete cds
ATGAAAAAAACAGCAATTGCATTGACTATCGTTGCACTAGCCGTGGCTTCAGTTGCACAAGCTGCACCAC
AACCTAACACATTCTATGTAGGTGCTAAAGCAGGTTGGGCATCTTTCCACGATGGTTTAAATCAAGCTAA
ATATCTTGAAGCTCCTGAGGCTACATTTGGCTTTAAACGTAATAGCGTTACTTATGGCGTGTTTGGTGAA
TATCAAATCACTGATAATTTTGCAGTAGAGTTAGGCTATGATGATTTTGGTCGTGCGAAGTTACGTATGG
CTGAAAAAGATCAAAAAGCAAAAGATGCAGCAAAACACACTAACCATGGTGCGCATTTAAGCTTAAAAGC
AAGCTATCCTGTACTTGATGGTTTAGATATTTATGCGCGTGTTGGAGCAGCATTAATTCGCTCAGATTAT
AAAGTGTATGATCATTCTGACCCAGCAAAATTACCACAATTTAAGAGAACTCATAGCACCCAAGTTTCTC
CAGTATTCGCAGGTGGTTTAGAATATGCGTTTATGCCTGAGTTAGCTTTACGTGTTGAATACCAATGGGT
AAACAATGTAGGTAAAGTTAAAGATGTTCTTGGAGAACGCGTAGATTACAGACCAGATATCGGCTCTGTA
ACAGCTGGTTTCGGTCAATCTGTTTATGTACCAGAAGTTGTGAGCAAAACATTCACATTAAACTCTGATG
TGACATTCGGTTTCGATAAAGCTGACTTAAAACCAGCTGCACAAAACGTGTTAGATGGTATCTACGGTGA
AATCGCCCAGTTAAAATCTGCCTCTGTTGCAGTTGCTGGTTACACAGACCGTTTAGGTTCTGATGCATAC
AACTTAAAATTATCACAACGTCGTGCTGACACTGTGGCTAACTACTTAGTAGCGAAAGGTGTTGCACAAA
ACGCTATCAGTGCAACAGGTCATGGTGAAGCGAACCCTGTAACTGGTAACAAATGTGATTCAGTTAAAGG
TCGTAAAGCACTTATCGCATGTTTAGCTGACGATCGTCGCGTTGAAATCGCTGTTAAAGGTAACAAATAA

Protein Sequence : Show Sequence

>AAT57676.1 OmpA [Pasteurella multocida]
MKKTAIALTIVALAVASVAQAAPQPNTFYVGAKAGWASFHDGLNQAKYLEAPEATFGFKRNSVTYGVFGE
YQITDNFAVELGYDDFGRAKLRMAEKDQKAKDAAKHTNHGAHLSLKASYPVLDGLDIYARVGAALIRSDY
KVYDHSDPAKLPQFKRTHSTQVSPVFAGGLEYAFMPELALRVEYQWVNNVGKVKDVLGERVDYRPDIGSV
TAGFGQSVYVPEVVSKTFTLNSDVTFGFDKADLKPAAQNVLDGIYGEIAQLKSASVAVAGYTDRLGSDAY
NLKLSQRRADTVANYLVAKGVAQNAISATGHGEANPVTGNKCDSVKGRKALIACLADDRRVEIAVKGNK

Molecule Role : Protective antigen
Molecule Role Annotation : DNA vaccines with OmpH and OmpA was superior to that provided by monovalent DNA vaccines.(Gong et al., 2013)
Related Vaccine(s): P. multocida DNA vaccine pcDNA-OMPH + pcDNA-OMPA , P. multocida DNA vaccine pcDNA-OMPH/OMPA

3. ompH

Gene Name : ompH
Sequence Strain (Species/Organism) : Pasteurella multocida strain X-73
NCBI Nucleotide GI : 2745957
NCBI Protein GI : 2745958
Protein Accession : AAC02243.1
Other Database IDs : CDD:225744
CDD:238208
Taxonomy ID : 747
Gene Strand (Orientation) : ?
Protein Name : outer membrane protein
Protein pI : 8.3
Protein Weight : 35024.16
Protein Length : 419
Protein Note : OmpH

DNA Sequence : Show Sequence

>gi|2745957|gb|U50907.1|PMU50907 Pasteurella multocida strain X-73 outer membrane protein (ompH) gene, complete cds
AAATATAAATAAAATTTGGGTGAAGTGAGAAATAGAGGCATTTACCCGATGTTTTTATCTTTGATTTATA
CTATTTTTGTTTTTAAATCAATGTGTTATGTTTTAAAAGAAAAGTTCTAAAGGAAATAAAATTTTTAATA
AATGTGATCTTTTTAACACTTTTTTAACCCTGAAAAAACCGCTGATTTTTTGTAACATGTTGTTTTTAAA
GGATAAGGTAAAAAAAAAGACAAACCTATTTTGTTTTGACAAGAAAACGGAGCTTTGCTATCTTGCATTT
GCAAAAATGTGCAGACGACTTATCGTCTGTTAGTCGATTTTAGGTTGATTGAGTGATTGGCAACAATCAT
TTAATCGAATTATAAAACTCCAAAAACAAGGTAGTGATACTATGAAAAAGACAATCGTAGCATTAGCAGT
CGCAGCAGTAGCAGCAACTTCAGCAAACGCAGCAACAGTTTACAATCAAGACGGTACCAAAGTTGATGTA
AACGGTTCTTTACGTTTAATCCTTAAAAAAGAAAAAAATGAGCGCGGTGATTTAGTGGATAACGGTTCAC
GCGTTTCATTCAAAGCATCTCATGATTTAGGCGAAGGCTTAAGCGCATTAGCTTATACAGAACTTCGTTT
TAGTAAAAATGTACCCGTGCAAGTAAAAGACCAACAAGGTGAAGTAGTACGTGAGTATGAGGTTGAGAAA
CTTGGTAACAATGTTCACGTAAAACGTCTTTATGCGGGTTTCGCGTATGAAGGTTTAGGTACATTAACAT
TCGGTAACCAATTAACTATCGGTGATGATGTTGGTCTATCTGACTATACCTATTTCAACAGTGGTATTAA
TAACCTCCTTTCTAGCGGTGAAAAAGCAATTAACTTTAAATCTGCAGAATTCAATGGTTTCACATTTGGT
GGTGCGTATGTCTTCTCTGCTGATGCTGACAAACAAGCATTACGTGATGGTCGCGGTTTCGTTGTAGCAG
GTTTATACAACAGAAAAATGGGTGATGTTGGTTTTGCATTCGAAGCCGGTTATAGCCAAAAATATGTGAA
ACAAGAAGTTGAACAGAATCCGCCAGCAGCACAAAAAGTGTTTAAAGATGAAAAAGAGAAAGCTTTCATG
GTGGGTGCTGAGTTATCATATGCTGGTTTAGCGCTTGGTGTTGACTACGCACAATCTAAAGTGACTAACG
TAGATGGTAAAAAACGTGCTCTTGAAGTGGGTTTAAATTATGACCTTAACGACAGAGCGAAAGTTTACAC
AGACTTCATCTGGGAAAAAGAAGGTCCTAAAGGTGATGTTACAAGAAACCGTACTGTCGCTGTAGGTTTT
GGTTACAAACTTCACAAACAAGTGGAAACTTTTGTTGAAGCAGCTTGGGGTAGAGAGAAAGACTCTGATG
GTGTAACAACAAAAAACAACGTAGTAGGTACAGGTTTACGCGTACACTTCTAATTTTTGTTAGAATCTGA
AAAAAGCCAGTGTTAAACACTGGCTTTTTATTGGGTTTTATTTGTTTTACTTACAATAAATTAGGATTTT
GAAAGTCGTTACGCGGTCATCTTTCTCAAAATAATACATATCAATATGTTGCAAGGAATGGATC

Protein Sequence : Show Sequence

>AAC02243.1 outer membrane protein [Pasteurella multocida]
MKKTIVALAVAAVAATSANAATVYNQDGTKVDVNGSLRLILKKEKNERGDLVDNGSRVSFKASHDLGEGL
SALAYTELRFSKNVPVQVKDQQGEVVREYEVEKLGNNVHVKRLYAGFAYEGLGTLTFGNQLTIGDDVGLS
DYTYFNSGINNLLSSGEKAINFKSAEFNGFTFGGAYVFSADADKQALRDGRGFVVAGLYNRKMGDVGFAF
EAGYSQKYVKQEVEQNPPAAQKVFKDEKEKAFMVGAELSYAGLALGVDYAQSKVTNVDGKKRALEVGLNY
DLNDRAKVYTDFIWEKEGPKGDVTRNRTVAVGFGYKLHKQVETFVEAAWGREKDSDGVTTKNNVVGTGLR
VHF

Molecule Role : Protective antigen
Molecule Role Annotation : rOmpH was used in a mice protection study. Mice immunized and challenged intraperitoneally resulted 100% protection against P. multocida whilst mice immunized subcutaneously and challenged intraperitoneally only resulted 80% protection (Tan et al., 2010).
Related Vaccine(s): P. multocida rOmpH Vaccine

4. ompH

Gene Name : ompH
Sequence Strain (Species/Organism) : Pasteurella multocida strain Pm-17
NCBI Nucleotide GI : 99083557
NCBI Protein GI : 99083558
Protein Accession : ABF66676.1
Other Database IDs : CDD:225744
CDD:238208
Taxonomy ID : 747
Gene Strand (Orientation) : ?
Protein Name : outer membrane protein
Protein pI : 9.15
Protein Weight : 34480.88
Protein Length : 413
Protein Note : Outer membrane protein (porin) [Cell wall/membrane/envelope biogenesis]; COG3203

DNA Sequence : Show Sequence

>gi|99083557|gb|DQ417897.1| Pasteurella multocida strain Pm-17 outer membrane protein (ompH) gene, complete cds
ATGAAAAAGACAATCGTAGCATTAGCAGTCGCAGCAGTAGCAGCAACTTCAGCAAACGCAGCAACAGTTT
ACAATCAAGACGGTACAAAAGTTGATGTAAACGGTTCTGTACGTTTACTTCTTAAAAAAGAAAAAGATAT
GCGTGGTGATTTAATTGATAACGGTTCACGCGTTTCTTTCAAAGCATCTCATGACTTAGGTGAAGGCTTA
AGCGTATTAGGTTATGCAGAACTTCGTTTTAGTAAAGATGTGAAAGATAAAGAGGGTAAAGTTAACCAAC
CAATCGGTAACGAAGTTCATGCTAAACGTCTTTATGCGGGTTTTGCGTATGAAGGTTTAGGTACATTAAC
TTTTGGTAACCAATTAACAATTGGTGATGATGTTGGTGTGTCTGATTACACTTATTTTAACAGTGGTATC
AATGGCGTACTTATTACTAGTGGTCAAAAAGCAATTAACTTCAAATCAGCAGAGTTCAACGGTTTCACAT
TTGGTGGTGCGTATGTGTTCTCAGGCGATGCAAACAAAGATGCATTACGTGATGGTCGCGGTTTCGTAGT
AGCAGGTTTATACAACAGACAAATCGGTGATGTTGGTTTTGCATTCGAAGCAGGCTATAGCCAAAAATAT
GTAAAACAGAAAGTTGAACAACCTCAGCCTTTACCTCCTGGTCAAGTAGAGCGTTTCAAAGATGAAAAAG
AAAAAGCTTTCTTGGTCGGTGCAGAATTATCGTACGCTGGTTTAGCGCTTGGTGTTGACTACGCACAATC
TAAAGTGACTAACGTAGATGGTAAAAAACGTGCACTTGAAGTGGGTTTAAACTATGACCTTAATGATAAA
GCGAAAGTTTATACAGACTTCATCTGGGAAAAAGAAGGTCCTAAAGGTGATGTTGAAAGAACTCGCACTG
TAGCTGTAGGTTTTGGTTACAAACTTCACAAACAAGTTGAAACCTTTGTTGAAGGTGCTTGGGGAAGAAC
AAAAGATGCAGATGGTAAAACAACAAAAGATAATGTAGTTGGTACAGGTTTACGCGTACACTTCTAA

Protein Sequence : Show Sequence

>ABF66676.1 outer membrane protein [Pasteurella multocida]
MKKTIVALAVAAVAATSANAATVYNQDGTKVDVNGSVRLLLKKEKDMRGDLIDNGSRVSFKASHDLGEGL
SVLGYAELRFSKDVKDKEGKVNQPIGNEVHAKRLYAGFAYEGLGTLTFGNQLTIGDDVGVSDYTYFNSGI
NGVLITSGQKAINFKSAEFNGFTFGGAYVFSGDANKDALRDGRGFVVAGLYNRQIGDVGFAFEAGYSQKY
VKQKVEQPQPLPPGQVERFKDEKEKAFLVGAELSYAGLALGVDYAQSKVTNVDGKKRALEVGLNYDLNDK
AKVYTDFIWEKEGPKGDVERTRTVAVGFGYKLHKQVETFVEGAWGRTKDADGKTTKDNVVGTGLRVHF

Molecule Role : Protective antigen
Molecule Role Annotation : DNA vaccines with OmpH and OmpA was superior to that provided by monovalent DNA vaccines.(Gong et al., 2013)
Related Vaccine(s): P. multocida DNA vaccine pcDNA-OMPH + pcDNA-OMPA , P. multocida DNA vaccine pcDNA-OMPH/OMPA

III. Vaccine Related Host Genes

1. IgG

Gene Name : IgG
Sequence Strain (Species/Organism) : Bos taurus
NCBI Gene ID : 281766
NCBI Protein GI : 116686102
Locus Tag : BOS_2620
Genbank Accession : GJ060600
Protein Accession : NP_001070870
Taxonomy ID : 9913
Chromosome No : 3
Gene Starting Position : 7996510
Gene Ending Position : 8005231
Gene Strand (Orientation) : +
Protein Name : transcript variant 2
Protein pI : 8.71
Protein Weight : 25616
Protein Length : 250
Protein Note : Also known as CD16; FCGR3; FCGR3@

DNA Sequence : Show Sequence

>gi|258513364:7996510-8005231 Bos taurus breed Hereford chromosome 3, Bos_taurus_UMD_3.1, whole genome shotgun sequence
CACTCTAACACACAGCATGTGGCAACTGCTACCACCGGCAGCTCTGCTGCTTCTAGGTAAGTCAGACTCA
CTTGGCCTGAGAGCAGAGCAAAGATGCCCTGGGCACAGCAGGCATCTCTTCTCAGGCAAGGAATGAGACC
CTTAACCAAGGACAGACCCCTTCCTATGTCTATAGACCTTGGTTTAGCTCCTAGGACACAGCTTCAGGGG
TTAGAGATACAGGAAATGGGCATAATCGGGAGGTGAAAGGAGAGGACGTGAGTTCTCCGTCACTTCACTC
CTCTAGGTCTCAGCTTCTCTGGCTATAAACTGACAAGTATGCACTGGGTAGCCCCTAAGGTCTGTCTCCC
ATTCTCACTTTCCAATGCTCCTCTCTCAGTCGTTTCATTTCTATCCTTTCCAGAGGACTTGGGTGGAGAT
AGTTTCCATGGCTTGATTCAGAGATATTCAACCTGTGCCCTTAACTTTGGGGTTTGAACAGGGACCAAGG
AGGAAGGGTATTGAAAACAGGGATTTGGGCAGACAACAGATTAGGAGGTACAGACGCTGGAAACCAGAAG
GCAGGCAGTGGGGGAGATACCCACTCTATTCATCTTTGTTGAAGATGTCTTTACCTTGTTTGTTTATTTA
ATGGCTATACGGGGCCTTCACTGTGGCGCTCAGGCTCTTCATCGCTTTGCGCGGGCTGCCTCTAGTGGCG
GAGCACAAGCCTCTTGTCGCGGTGGTCTCTCGTCGCGGTGGTCTCTCTTGTTGTGGAGCACAGGCTCTAG
GGTGCTCAGGCTTCAGTAGTTGTGGCTCATGGGCTCTAGAACATGAGCTCTGGTTGCTCTGCAGTGTGTG
GGATCTTACTTCTGGCGCCAGGGATTGAACCTGCGTCCCCTTTACTGGCAGGCGGATTCTTACCACCAGG
GAAGTCCCCACTATCTTTATCCTGATTCTTGGGAACACTGGCCTCTGAGGGCAGAGGTGGGCAGGGGAGT
TTAAATACCACACTCCAGTCACCCTGTTCTCTGCTGCTGACCAATATTTGTTTTATTCTTTTTTTCTTAC
AGTTTCAGCTGACACGCAAACTGGTGAGTTTGCTTCATGGTCTTGGACGGATCGAGTGAGGCATGTAAAT
GTGTAATAGCTGCAGGGCACTGTTAGGAAATGTTCTTTGCACAAGAAACTGTTGACATGGAGTTGATGGT
GGTGGTGGTGGTGGTTTAGTCGCTAAGTTTTGTCTGACTTGCAACCCCATGGACTATAGCCTTCCAGGCT
CCTCTGTCCATGGGATTCTCCAGGCAAAATATTGGAGTGGATTGCCATTTCCTTCTCCAGAGGATCTTCC
CGACCCAGGGATTGAAACCAGGTCTCTTGCATTGCGGGCAGATTCTTGGCAGGGGCAATTCCCCCAATAC
AGTGGGCTTGGCCCTACCAAGGAGGAGGCTAAGTCTCTCCAAGTCAGTATGCTCAGACTGAGTGCAATGC
TGCTGCTGCTGCTGCTGCTGCTGCTAAGTCACTTCAGTCGTGTCCGACTCTGTGCGACCCCACAGACGGC
AGCCCACCAGGCTCCGCCGTCCCTGGGATTCTCCAGGCAAGAACAGTGGAGTGGGTTGCCATTTCCTTCT
CCAATGCGTGAAAGTAAAGTCGCTCAGTCGTGTCCAACTCTTAGCGACCCCATGGACTGCAGCCTACCAG
GCTCCTCCATCCATGGGATTTTCCAGGCAAGAGTACTGGAGTGGGTTGTCATTGCGTTCTCCAGACTGAG
TGCAATACTAAGGCTGAAATGCCAACTCTCAGTTTGCAATGGGTTTTGAGCCTCCTTGGGAATACCAGTA
TAGAGCCTGCTCACTTCTCTGCCCTTATCTACATTCTCTTAACACTTCAGCAGATCCCTCAAAGGCTGTG
GTGCTCCTAGACCCTCAGTGGAACCACGTGCTCACGAATGACCGTGTGACTCTGAAGTGCCAGGGAGACT
ACCCTGTTGAAGACAATTCCACAAAGTGGTGGCACAATGGGACTCTCATCTCAAGCCAGACCCCCAGCTA
CTTCATCGCAGATGTCAAGGTTCAGGACAGTGGCGAGTACAAGTGTCAGACAGGCCTCTCTGCACCCAGT
GACCCGGTGAAGCTAGAAGTCCACGTAGGTGAGTAGGAGAAGGGGAAAAAGGAACTGACCAATAAAGGAT
AAAAAAAAAAAGAGGCCCTGAAAAAAAATAATGTTCCTGGGGATTAAAACTAGGATGAGATGATCTGTAG
TCTATTGAATTACATCAGTATTGTAGTCCTAGCTCCAAGTAGGCCTTAGATAAAATGTCAAAGCCTGGCA
TACACTAGGGACAGGGCTGCTGGACTGCACAATTCCAAGAATCTACATTCACATAGAAGATGAATATATA
CAGCATGAATGGTGTCATCTGAAGGTCAGGGCATGACATCAATGCTCTATAACAGTTATTGAATAAATTA
ATGAATCCACTTTAGGACCTCAATCTCCTCATTCTCAGACACAATCTCCACATTCTCTAAGCTACCCAAC
AAGCCACCCCTAATCTAGCATCATATCTATTTCCTCTTCCCATCAACGCTACACTCAAATATACACTTCA
GCCTAAATTTGGCAATGTGAAATCTCTCCTAATAGAGATAGATACACGTTCAGCCCGCACATTCATCATG
GAACGAAAGTCTTTGTATCTTAACAAAATCCAGGCATTGGATGCAAGGAGAAGTATGTTGAGGCAGAGAT
CTCTCCTGGTGTCCCTGAAATCACACCACATAGCCTGGTACCTGCTGTGTCTTGTAAGCTTCACTCTATT
TGCATGACTGCCCCATCTCCATAATGTATGTGAGTGGAACAAGCCTGGAGGGAAAGGCGGGTTTAATGAA
GGAGGACCTGCAACAAAATTCCACGGACCTGGGTCTCTGAAGCTGATTTTCTGGGCTCCTACACCTGACT
GTGTTACTTAGTTGACTTAGTTCTCAGTCTAGCTAGGTGAGAGATGGCCATGCCTGTGTGCCAAGTTGCT
TCAGTCATATCCGACTCTTTGTGACCCTATGGACTGTAGCCTGCCAGGCTCCTCTGTCCATGGGATTCTC
CAGGCAAGAATACTGGAGTGGATGGCCATGTCTTCCTCCAGGGGATCTTCCCAACCCAGGGATCTAAGCC
ACATCTCTTACATCTCGTGCTTTGGCAGGCAGGTTCTTTACCACTAGCACCACCTGGGAATCCCAAGAGA
TGGCCACCTGTGACCAAATAAAATGGGGAATTACCAACAGGAAGCAAGAGAAACCTCAAGTCTTGGAGTT
GTTAGTTTGTCTTAAACTTGGCTCTAGAAACTGACTTTAGAAGTCTAATTAGTGTGAACAGTTTTAAACT
CTGACCAAGAGCATTAAGTTCACCAGGCAAACTCCTGATCCTTCTTTTGGACCCACTTCACTTATCTTCT
TCTCTGAGAAGCTTTGCTTACTTTCTTCGGGCCGGATTTCTTTGCTCCATTCTCTTTGGTCATGCAGTCC
TTTGTTCTTAGCCTGTTGTAGCACTTATCACCTGGTATTATAATTATTTCTGAGTATATGTGTTTCTACG
CCTAGACCACAGGTTCTTTGATGTGTCTCAGTCATCTGGGTGTCCCCAATCTGCATGACATGACATAGTA
AGTTCTCAATAAGATATTTGTTTAACTAATGTATATATGAACATTCACTGAGTCCATGACACCCTGAATG
GAATAAGAACGTTCTCCCCTCAAAGAAACCAAACTATATGTGTGTGTGTGTGTGTGTGCGTATATGTGTG
TATATACAACATAAGTTATGTATATATATGTGTGTGTGTATGTATGTATATATATGATTACATATGTAAC
CCCTGTTAATATTTAAGCACATGTTCTTTCTTTTTTTATGAGCATACATATGTTTTATATTCTTTTTAAC
TTAACATTCTCTGATAAATGTTTTCTCATGTCATTTTAAAATTTCTCTGCAGTATCGTTTTAAATTATTC
AGGCCAACTTCCTCACCATGACTTCAAGTTCTCTGTGTGTGTGAAGTCTGGTTACTTGGCAAGGCAGTTG
TGAAAAGAGTTGTTTTGATGATTAACTGTTTCCGTCTTAAAGGCTGTGCTCTGTAATGAAATGGTCTTGC
TAAGACACTGTGGCCCCTGAAATTAGCAGTTACCATCAGCACTACCAAGCATGCTTTTCTTTCCTCTCCC
TGACTTTTACAAGGAGTTCTCCTTCACAATTTCTGTAGCCACTCCTACGGATACTCAGTAACCTTCTTCC
TGACTGGTTGCAACAGTCTTCCAACATTCCTAAAACCGTCTATTCCTTCTGAAGTCTACCTTTCCTTGTA
TTCTAATATGATTCTTACTCTTTGATCTAGAATTTACAATGGCCCCTTATCACCTTCCATAGCAAATCCA
AGTGTGGACTTTGAGGATGCTGCAGCTTGCTTCTCACCTGCCTCATCACTTAGTCCTTCTGCTCTCACTT
TATGCTTTGATCATGTGTGTGTCTGTCCCTCTCTCCCATTAGACAAAAGCTCTTGGAGTGACTTGAGTTC
TCATCCAGAGTCCAGTTGTCTTTTTCCCAAAGAAAGTTACATAAGGACGGCGCCGCCCGGTCTGGAGTTC
CACTGTCCCCCAGCACCTGGGTGGTGAGCATTCTCTTCAGAAGAGCTTTGTGCTGGGTGCTGAGGCCTCC
TGGGCCTGTGTTCTCTTTGTGTTTTGCAGGCTGGCTATTGCTCCAGGTCGCTCAACGGGTGGTAAATGTG
GGAAAGCCCATTCGGCTGAAGTGTCACAGCTGGAAGAAAACTCCTGTAGCAAAGGTCCAGTATTTCCGGA
ATGGCAGAGGCAAGAAGTATTCTCATGGGAATTCTGACTTCCACATTCCAGAAGCAAAACTTGAACACAG
TGGTTCCTACTTCTGCAGGGGCATTATCGGGTCTAAAAACGAGTCTTCAGAGTCTGTGCAGATCACTGTT
CAAGGTAAGACATGTGCGGCACTAAAGGGACCAGAGACCCCTGTGCTGGGAATCTTGCATTCACACTGGG
GAGATGAGTTGGGAGGAAATTGCTGACAATTCCACAGACTTCTTTACGAGTGTCATTCACTTTGAAACAT
GACAGCAGGTGCAAAGGCCACATTTCCTTCCTCATAAATATGCCTGTCCTAACTTACCTCATTCAAACCA
TATCCACACTCTTGTGTATCTCTATCGTGCTGGCCCTTTTTTTGCCTGCTCCTGCTCCCCTTTCTCTTCC
AAGTCTCAAGTCTTAGCCAAGAGTCTAGCTGGCAGTAACCCCTCACTTTCTCACAGAAAGACCCAGAACC
TGTGTTCTGGCTCTGCTGCTGATTAACCATATGACTGTGGCCAGTTAGTCTCTTGAACTCAATTTCCACT
TTTGTAACAAAGACAAGCTGACGTTTATTGGGCATTTTTCAGTGTGCTGGACACTGTGCTGAATGCTTCA
TTACATGCACTTAATCTGAACACCTGAGAATTAATGCTTTTGAGCTGAGGTGTTGGAAAAGACTCTTGAG
AGCCCCTTGGACAGCAAGGACAGTCAATCCTAAAGGAAATCAGTCCTGAATATTCATTGGAAGGACTGAT
GCTGAAGCTGAAACTCCAATCCTTTGGCCACCTGATGAGAAGAACTGACTCATTGGAAAAGACCCTGATG
CTGGGAAAGATTGAAGCAGAAGAAGAAGGGATGACAGAGGATGAGATGATTGGATGGCCTCACCGACTCA
ATGGCCATGAGTTTGAGTGAGCTCTGGGAGTTGGTGATGGACAGGGAAGCCTGGCGTGTTGTAGTCCATG
GGGTCACAAAGAGTCGGACACGACTGAGTGACTGAACTGAACTGATATTGAATTCCACTTTCCAAATCAT
TTAGAAAACATCTCCAGTTTCTGTAGCACCATCCTCCAGGGTCAGAGATGCCCTGCCATGGTGCTCCTGG
ACTTCCTAATCTCCCAAAACTTCCACCTGGAATCAGACTGAGATAAAAACCCTGATACTGGGAAAGATTG
AGGGCAGGAAGAGAAGGGGGCGACAGAGGATGAGATGGTTGGATAGTGTCATTGACTAAACGGACATGAG
TTTGAGCAAACTCTGAGAGATAGTGGAGGACAGTTAAGCCTGGCATGCTGCAGTCCATGGGGTCACCAAG
ATTCGACACGACTGAGTGACTGAACAACAATACACTGTAAGCAATTTACATGTAATAATACAGCAATATA
GACACACATCTTCTGAGATTCTAACAAAACAATGCCATAGTTTGTATAAAAAATAACTTCATCACAATTT
AAATTGAAAGTAAATACTCTCAAATAGCTAGGAAAAGAGAGAGCAAATAATAAGGAAAATGGGGGCAAAT
TGCAAACAATTAGTAAATCTAGGTAAAGAATATCTGAGAGTTATTTGTACTTTATTGACATCCATTTTTT
AAGTTCAAAATTATATCAAAATTAAGATTTTTTAAATTGAGACTAAAAATGCACTCTCATCTTACTACCT
TATAGGCAACAGCAGAGAATGACTTTAAAACAATCTTAATGGCTAAGAGGTTGAGAAGCAACAAATTCTA
GCCCTTTCCATTTTGGGTTCTTAGTTACTGAGCCTTGATAAAGAAATATTTTCTGACGATAAATAGGATA
AGTAGTAGATTTCAACGGCTTACCTTGTGTGTGTGTTGGTTTTTGTTTTTGTTTTTTCATTTTCTTGAGT
CGGGTGAATACAATGAGTATAATTTTGAGACACAAGAGAAAGTTACTGAATTAAAAAACAGTGTTCTCAC
AACAACTTAAACTTTGATATCTCTCATTGCTGTTGTACAGTCACTAAGTCATGTCCGACTTTTTGCAGCC
CCATAGACAGCATGCCAGGCTTCCCTGTCCTTACCTAACTCCCAAGTTTGCTCAAACTCATGTCTATTGA
GTTAGACATCTCATCCTCTGTCACCTCCTTCTCCTTCGCATCAGGTGGCCAAAGTATTGGAGCTTCAGCT
TCAGCATCAGTCCTTCCAATGAATATTCAGGGTTGATGATCTCACGCAAAACCCAGCTACAAGTACCTCC
ATCAAACATGAAAACACTATTGCTCAAGAAGCACTAGAATCCAAAACTGGTAGGAGGAAGCTGTGGCTTT
CTTCCAGACTCCCCCACATTCAATAGAATCTGTTTCTTAGCAGGTGTCAGAAAGACTTCACAGTGATGAC
CTCACTGTTTATGCCAATTTTCTGCCTTAACAAATGTCATTTTTTTCTCATCTCTCTGCTCAGCTCCAGA
AACTTTACAAACTGTCTCGTCATTCTTTCTACCTTGGCACCAGATCACCTTCTGTCTGGTGATGGGAGTC
CTGTTTGCAGTGGACACGGGACTGTATTTTTCTGTACGGAGACACCTTCAAAGCTCAGAGGAATGGAGGG
ATGGCAAAGTCACATGGAGCAAGGGCCCTTAGGACAAATGGAGGTAATGAAAGCAGCATCCCCATCCCAT
GGGGTGATAAAAGCAGCATCTATGAACCTCTTCCTAATTTCACAGCCTTATCATCTCCCAACAGGCATCT
CTGGGAGCAGCAGGAAAATTACACGTCAGACTCTAGGGATCTGAGGATCCTTCACCCAACTCTGTTTCTT
CTAGTCTCCTATGGAAAGAAAGTGCCAACCGTGAAGGACCAGTAAATATCTGCATCACCAGAAATAGAAG
TCTCAGAGCTACGTGGCTGCTTTCTGTATTCCAACCATTCTGTCCCAAGAGAACAACCATACAGACTCTC
AAAGATCAGCCCTGATGTATGAATATTGTGCTAAAATAAATGGATATGTAAAGAAGAAAATTCATGGTGG
AGAGGCAGCTGGATGGTGGTCTACATCTTTTGCTGCACGCTGACTGCCTTCAAGGCACCCTGAAACTCCT
TCTAGATAGTCTGTGGGTACATGTGGGGGTGTAGGGAACAGAGCACAACCATTGAAGAGGGTGGCTGAGA
AAGAGCAGGGTAGTCCAGGATAGACCAGACAAGACCACCACCTGGGCTGTCTTCCTAGAACATGAACCAT
GGTGGAGGTAAAAGAAAATCACAGACGTGAGATGGGATTGAGTTTTCCAGGGGAGCCCCTGTCAAAAGGA
TACAGTGTGAGTCTGTGAGTATACAATGATAAATCCTCTCAATGTCATGAGTAGAAAATGATCCAGGAAG
GGGGTTAGGGGTTGGAGGATTTGCAAAAGAAAGCACAGAATAAACTCATTGGAAGGAACATTGCTATCCC
AAATTAAGCCCACTGAACAAAGGTATATCAGTGTCTAAACCGAGAAAGCATTACAAGAAAAAGTCAACCA
CAACTGCACAGGAAGGAGCTGGCAGGAGATGAGGATGCTTTTAGACTGAGAATTAGAGAGAAGGGGCATA
TAAAACCAGAATTTTCACTTCAATATCTAATTCAGCTTTATTCACATCACCCTTAAACAAATGTTGGATA
ACCACTATTAAATGTACTAAACTCTACTGAATTAAATCATGAAACTTTTAAATCCTTCAGCATGTCAATA
ATAATGGAGTAGAGGTGAAATCAATCACTGCTTCTGGAAGAAAGCTTTAGCTATCTCTCTTTGTGTTTTA
GAAGCTTTAAACTGAACATTTCTTGATTCCGGTAAGGCATTTTTACAAGATTCTGCATGTTATTGTTAGA
TAGAAGATGGAAAAACCACGATAATAAAACATGAATGATAGA

Protein Sequence : Show Sequence

>gi|116686102|ref|NP_001070870.1| low affinity immunoglobulin gamma Fc region receptor III isoform 1 precursor [Bos taurus]
MWQLLPPAALLLLVSADTQTADPSKAVVLLDPQWNHVLTNDRVTLKCQGDYPVEDNSTKWWHNGTLISSQ
TPSYFIADVKVQDSGEYKCQTGLSAPSDPVKLEVHVGWLLLQVAQRVVNVGKPIRLKCHSWKKTPVAKVQ
YFRNGRGKKYSHGNSDFHIPEAKLEHSGSYFCRGIIGSKNESSESVQITVQAPETLQTVSSFFLPWHQIT
FCLVMGVLFAVDTGLYFSVRRHLQSSEEWRDGKVTWSKGP

Molecule Role : Vaximmutor
Related Vaccine(s): Pasteurella multocida aroA mutant vaccine

2. IgM

Gene Name : IgM
Sequence Strain (Species/Organism) : Bos taurus
NCBI Gene ID : 444875
Locus Tag : BOS_20600
Genbank Accession : GJ062732
Taxonomy ID : 9913
Chromosome No : 21
Gene Starting Position : 71507508
Gene Ending Position : 71508717
Gene Strand (Orientation) : +
Protein Name : IgM

DNA Sequence : Show Sequence

>gi|258513346:71507508-71508717 Bos taurus breed Hereford chromosome 21, Bos_taurus_UMD_3.1, whole genome shotgun sequence
AGTGACTTCTGGGAGTGGTGACTCTCATCTGCTCGAAGATGAACCCACTGTGGACCCTCCTCTTTGTGCT
GTCAGCCCCCAGAGGTGAGTGTCTCTGGGTCAGACATGGGCACGTGGGGAAGCTGCCTCTGAGCCCACGG
GTCACCGTGCTTCTCTCTCTCCACAGGGGTCCTGTCCCAGGTGCAGCTGCGGGAGTCGGGCCCCAGCCTG
GTGAAGCCCTCACAGACCCTCTCCCTCACCTGCACGGTCTCTGGATTCTCATTAAGCGATAATAGTGTAG
GCTGGGTCCGCCAGGCTCCAGGAAAGGCGCTGGAGTGGCTCGGTGTCATATATAGTGGTGGAAGCACAGG
CTATAACCCAGCCCTGAAATCCCGGCTCAGCATCACCAAGGACAACTCCAAGAGCCAAGTCTCTCTATCA
CTGAGCAGCGTGACAACTGAGGACACGGCCACATACTACTGTGCAAGAGACACAGTGAGGGGAAGTCAGT
GTGAGCCCAGACAAAAACCTCCCTGCACAGGGGCCCGTGACCACGAGGGGGCGCTCAGGACTCAGTACAG
AGCTGAGTCCTGGAGCAGGTACGGAAGAGGCGTTGGGCGGTGGGGGTCCCGGGGGGGATTCTCCTCAGAG
CTTCTCTTTGCTCCTCCTGCCCAGGAGCTGCAGCATAGACCCATTTCTGTGTTCTGGTATTTCATTGTTG
TGGTTTATGTTACTCTCAGACAACTGTGTGTATATATATATTTCATTTTATCTTGAAGCAAGGATAGCTT
TAGGAAAACATACAGTCACACATAATACTCAACAGCTAAATTTTTTCTCCTTCATTTTTATTCCTTCAAG
GGAACTCAGTAAAACACTTGCTGCTGATACTGCTAAGTCGCTTCAGTTGTGTCCGACTCTGTGTGACCCC
ATAGATGCCAGCCCATTAGGCCCCACGGTCCCTGGGATTCTCCAGGCAAGAACACTGGAGTGGGTTGCCA
TTTCGTTCTCCAATGCATGAAAGTGAAAAGTGAAAGTGAAGTCGCTCAGTCCTGTCCAACTCTTCACGAC
TCCATGGACTGCAGCCTACCAGGCTCCTCCGTCCATGGGATTTTCCAGGCAAGAGTACTGGAGTGGGGTG
CCATTGCCTCCTCCGTCAGTAAAACCCTTGACTCTCGATAATTTCAAAACTGTTAACTATCCTGAAAGGA
AACATAAGATATTTAAACAT

Molecule Role : Vaximmutor
Related Vaccine(s): Pasteurella multocida aroA mutant vaccine

IV. Vaccine Information

1. Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45H5.20)

a. Manufacturer:

Texas Vet Lab, Inc.

b. Vaccine Ontology ID:

VO_0001877

c. Type:

Inactivated or "killed" vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Cattle

2. Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45H5.21)

a. Manufacturer:

Texas Vet Lab, Inc.

b. Vaccine Ontology ID:

VO_0001878

c. Type:

Inactivated or "killed" vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Cattle

3. Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 4109.20)

a. Manufacturer:

Texas Vet Lab, Inc.

b. Vaccine Ontology ID:

VO_0001879

c. Type:

Inactivated or "killed" vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Cattle

4. Bovine Rhinotracheitis-Parainfluenza 3 Killed Virus Vaccine-Mannheimia Haemolytica-Pasteurella Multocida Bacterin (USDA: 4265.00)

a. Manufacturer:

Boehringer Ingelheim Vetmedica, Inc.

b. Vaccine Ontology ID:

VO_0001885

c. Type:

Inactivated or "killed" vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Cattle

5. Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 43S5.20)

a. Manufacturer:

Texas Vet Lab, Inc.

b. Vaccine Ontology ID:

VO_0001905

c. Type:

Inactivated or "killed" vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Cattle

6. Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 43T5.20)

a. Manufacturer:

Texas Vet Lab, Inc.

b. Vaccine Ontology ID:

VO_0001906

c. Type:

Inactivated or "killed" vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Cattle

7. Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45B9.20)

a. Manufacturer:

Boehringer Ingelheim Vetmedica, Inc.

b. Vaccine Ontology ID:

VO_0002006

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Cattle

8. Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45B9.21)

a. Manufacturer:

Boehringer Ingelheim Vetmedica, Inc.

b. Vaccine Ontology ID:

VO_0002007

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Cattle

9. Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus-Mannheimia Haemolytica-Pasteurella Multocida Modified Live Virus, Avirulent Live Culture Vaccine (USDA: 11A8.22)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0002008

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Cattle

10. Bovine Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 4C95.20)

a. Manufacturer:

Texas Vet Lab, Inc.

b. Vaccine Ontology ID:

VO_0002024

c. Type:

Inactivated or "killed" vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Cattle

11. Mannheimia Haemolytica-Pasteurella Multocida Avirulent Live Culture Vaccine (USDA: 1861.01)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0002029

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Cattle

12. P. multocida DNA vaccine pcDNA-OMPH + pcDNA-OMPA

a. Vaccine Ontology ID:

VO_0004536

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Chickens

e. Gene Engineering of OmpA

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

f. Gene Engineering of ompH

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

g. Vector:

pcDNA3.1(+) (Gong et al., 2013)

h. Immunization Route

Intramuscular injection (i.m.)

i. Chicken Response

Vaccine Immune Response Type: VO_0000286
Immune Response: A significant increase in serum antibody levels was observed in chickens vaccinated with divalent combination. Additionally, the lymphocyte proliferation (SI value) and the levels of IFN-γ were both higher in chickens immunized with divalent combination than in those vaccinated with monovalent DNA vaccines (P<0.05) (Gong et al., 2013).
Efficacy: The protection provided by divalent combination vaccine was superior to that provided by monovalent DNA vaccines after challenging with the avian P. multocida strain CVCC474 (Gong et al., 2013).

13. P. multocida DNA vaccine pcDNA-OMPH/OMPA

a. Vaccine Ontology ID:

VO_0004537

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Chickens

e. Gene Engineering of OmpA

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

f. Gene Engineering of ompH

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

g. Vector:

pcDNA3.1(+) (Gong et al., 2013)

h. Immunization Route

Intramuscular injection (i.m.)

i. Chicken Response

Vaccine Immune Response Type: VO_0000286
Immune Response: A significant increase in serum antibody levels was observed in chickens vaccinated with the fusion DNA vaccine. Additionally, the lymphocyte proliferation (SI value) and the levels of IFN-γ were both higher in chickens immunized with the fusion DNA vaccine than in those vaccinated with monovalent DNA vaccines (P<0.05) (Gong et al., 2013).
Efficacy: Protection provided by the fusion DNA vaccine was superior to that provided by monovalent DNA vaccines after challenging with the avian P. multocida strain CVCC474. The protective efficacy in chickens immunized three times with the fusion DNA vaccine was equivalent to the protective efficacy in chickens vaccinated once with the attenuated live vaccine (Gong et al., 2013).

14. P. multocida rOmpH Vaccine

a. Vaccine Ontology ID:

VO_0004221

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

100μg of rOmpH (Tan et al., 2010).

e. Gene Engineering of ompH

Type: Recombinant protein preparation
Description: The P. multocida ompH gene was cloned into E. coli and then extracted and purified to result in rOmpH (Tan et al., 2010).
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0000133
Description: Freund's adjuvant

g. Immunization Route

subcutaneous injection

15. Pasteurella multocida aroA mutant vaccine

a. Vaccine Ontology ID:

VO_0002862

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Cow

e. Gene Engineering of aroA

Type: Gene mutation
Description: This aroA mutant is from Pasteurella multocida (Dagleish et al., 2007).
Detailed Gene Information: Click here.

f. Immunization Route

Intramuscular injection (i.m.)

g. Cattle Response

Persistence: An aroA mutant is highly attenuated in calves (Dagleish et al., 2007).
Efficacy: An aroA mutant induces significant protection in calves from challenge with wild type Pasteurella multocida (Dagleish et al., 2007).
Host Gene Response of IgG
- Gene Response: After second vaccination, serum IgG titers rose. Groups receiving 10^9 and 10^8 CFU of the mutant had significantly higher titer of IgG than those receiving 10^7 CFU, and all vaccinated groups had significantly higher titers than the unvaccinated challenge control group of calves (Dagleish et al., 2007).
- Detailed Gene Information: Click here.
Host Gene Response of IgM
- Gene Response: After the second vaccination, serum IgM titers rose. Groups receiving 10^9 and 10^8 CFU of the mutant had significantly higher titer of IgM than those receiving 10^7 CFU, and all vaccinated groups had significantly higher titers than the unvaccinated challenge control group of calves (Dagleish et al., 2007).
- Detailed Gene Information: Click here.

16. Pasteurella Multocida Avirulent Live Culture, Avian Isolate Vaccine (USDA: 1871.01)

a. Manufacturer:

Biomune Company

b. Vaccine Ontology ID:

VO_0001722

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

17. Pasteurella Multocida Avirulent Live Culture, Avian Isolate Vaccine (USDA: 1871.02)

a. Manufacturer:

Lohmann Animal Health International, Arko Laboratories Ltd., Biomune Company

b. Vaccine Ontology ID:

VO_0001723

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

18. Pasteurella Multocida Avirulent Live Culture, Avian Isolate Vaccine (USDA: 1871.03)

a. Manufacturer:

Intervet Inc., Arko Laboratories Ltd., Biomune Company

b. Vaccine Ontology ID:

VO_0001724

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

19. Pasteurella Multocida Avirulent Live Culture, Avian Isolate Vaccine (USDA: 1871.04)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0001725

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

20. Pasteurella Multocida Avirulent Live Culture, Avian Isolate Vaccine (USDA: 1871.05)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0001726

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

21. Porcine Rotavirus-Transmissible Gastroenteritis Modified Live Virus Vaccine-Bordetella Bronchiseptica-Clostridium Perfringens Type C-Erysipelothrix Rhusiopathiae-Escherichia Coli-Pasteurella Multocida Bacterin-Toxoid (USDA: 49T9.21)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0002319

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Pig

V. References

1. Dagleish et al., 2007: Dagleish MP, Hodgson JC, Ataei S, Finucane A, Finlayson J, Sales J, Parton R, Coote JG. Safety and protective efficacy of intramuscular vaccination with a live aroA derivative of Pasteurella multocida B:2 against experimental hemorrhagic septicemia in calves. Infection and immunity. 2007; 75(12); 5837-5844. [PubMed: 17875632].

2. Gong et al., 2013: Gong Q, Qu N, Niu M, Qin C, Cheng M, Sun X, Zhang A. Immune responses and protective efficacy of a novel DNA vaccine encoding outer membrane protein of avian Pasteurella multocida. Veterinary immunology and immunopathology. 2013; 152(3-4); 317-324. [PubMed: 23340446].

3. Harper et al., 2006: Harper M, Boyce JD, Adler B. Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS microbiology letters. 2006; 265(1); 1-10. [PubMed: 17107417].

4. Tan et al., 2010: Tan HY, Nagoor NH, Sekaran SD. Cloning, expression and protective capacity of 37 kDa outer membrane protein gene (ompH) of Pasteurella multocida serotype B:2. Tropical biomedicine. 2010; 27(3); 430-441. [PubMed: 21399583].

5. Wiki: P. multocida: Wiki: Pasteurella multocida [http://en.wikipedia.org/wiki/Pasteurella_multocida]