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Pathogen Page

Gallid herpesvirus 1

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. gB (Protective antigen)
2. UL27 (Protective antigen)
3. UL0 (Virmugen)
4. UL23 (Virmugen)
5. UL47 (Virmugen)
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

10386

2. Disease:

Avian infectious laryngotracheitis, Fowl Laryngotracheitis

3. Introduction

Infectious laryngotracheitis (ILT) is a worldwide occurring respiratory disease of chickens, which is caused by a virus designated as ILTV or gallid herpesvirus 1 (GaHV-1). Infectious laryngotracheitis virus (ILTV) is an alphaherpesvirus that causes an economically important chicken disease, which results in delayed growth, reduced egg production, and also frequently in death of the animals. After acute infection of the upper respiratory tract, the virus can establish latency in the central nervous system, and subsequent reactivations can lead to infection of naive chickens. For prevention of ILT, conventionally attenuated live vaccines are available. The severity of clinical symptoms depends on the virulence of a particular strain or isolate, and mortality rates can vary between 0 and more than 70%. Symptoms of milder forms of ILT are nasal discharge, conjunctivitis and reduced egg production, whereas severe forms are additionally characterized by gasping, coughing, expectoration of bloody mucus, and marked dyspnoea which may lead to suffocation. Pathomorphological alterations can be observed in conjunctiva, larynx, trachea, and sometimes also in the lungs and air sacs (Fuchs et al., 2007).

4. Host Ranges and Animal Models

Like other avian herpesviruses, ILTV exhibits a very narrow host range both in vivo and in vitro. Besides domestic chickens, pheasants represent the only natural hosts. Furthermore, the virus could be isolated from peafowl, and experimental infections of turkeys have also been described (Fuchs et al., 2007).

5. Host Protective Immunity

ILTV infection of chickens frequently induces production of virus-neutralizing antibodies, but antibody titers correlate only poorly with protective immunity (Fuchs et al., 2007).

II. Vaccine Related Pathogen Genes

1. gB

Gene Name : gB
Sequence Strain (Species/Organism) : Gallid herpesvirus 1 isolate CG
NCBI Protein GI : 110676569
Other Database IDs : CDD:223014
Taxonomy ID : 10386
Gene Strand (Orientation) : ?
Protein Name : glycoprotein B
Protein pI : 6.87
Protein Weight : 93635.88
Protein Length : 942
Protein Note : glycoprotein BALF4; Provisional

Protein Sequence : Show Sequence

>ABG85153.1 glycoprotein B [Gallid alphaherpesvirus 1]
MASLKMLICVCVAILIPSTLSQDSHGIAGIIDPRDTASMDVGKISFPEAIGSGAPKEPQIRNRIFACSSP
TGASVARLAQPRHCHRHADSTNMTEGIAVVFKQNIAPYVFNVTLYYKHITTVTTWALFSRPQITNEYVTR
VPIDYHEIVRIDRSGECSSKATYHKNFMFFEAYDNDEAEKKLPLVPSLLRSTVSKAFHTTNFTKRHQTLG
YRTSTSVDCVVEYLQARSVYPYDYFGMATGDTVEISPFYTKNTTGPRRHSVYRDYRFLEIANYQVRDLET
GQIRPPKKRNFLTDEQFTIGWDAMEEKESVCTLSKWIEVPEAVRVSYKNSYHFSLKDMTMTFSSGKQPFN
ISRLHLAECVPTIASEAIDGIFARKYSSTHVRSGDIEYYLGSGGFLIAFQKLMSHGLAEMHLEEAQRQNH
LPRGRERRQAAGRRTASLQSGPQGDRITTHSSATFAMLQFAYDKIQAHVNELIGNLLEAWCELQNHQLIV
WHEMKKLNPNSLMTSLFGQPVSARLLGDIVAVSKCIETPIENIRMQDSMRMPGDPTMCYTRPVLIFRYSS
SPESQFSTNSTENHNLDILGQLGEHNEILQGRNLIEPCMINHRRYFLLGENYLLYEDYTFVRQVNASEIE
EVSIFINLNATILEDLDFVPVEVYTREELRDTGTLNYDDVVRYQNIYNKRFRDIDTVIRGDRGDAIFRAI
ADFFGNTLGEVGKALGTVVMTAAAAVISTVSGIASFLSNPFAALGIGIAVVVSIILGLLAFKYVMNLKSN
PVQVLFPGAVPPAGTPPRPSRRYYKDEEEVEEDSDEDDRILATRVLKGLELLHKDEQKARRQKARFSAFA
KNMRNLFRRKPRTKEDDYPLLEYPSWAEESEDE

Molecule Role : Protective antigen
Related Vaccine(s): Laryngotracheitis DNA Vaccine encoding gB

2. UL0

Gene Name : UL0
Sequence Strain (Species/Organism) : Gallid herpesvirus 1
NCBI Gene ID : 3239037
NCBI Protein GI : 148828637
Locus Tag : GaHV1_gp60
Genbank Accession : X97256
Protein Accession : YP_182395
Taxonomy ID : 10386
Gene Starting Position : 106974
Gene Ending Position : 109923
Gene Strand (Orientation) : -
Protein Name : protein UL0
Protein pI : 6.59
Protein Weight : 53811.98
Protein Length : 506
Protein Note : UL0 family

DNA Sequence : Show Sequence

>gi|57790931:106974-109923 Gallid herpesvirus 1, complete genome
ATTTATTGTTTATCTCGCAATTTTACATATATTTTAACAGCAACAAGCTCCGCCCCAAAATAAACACCGG
CGGAAAAAACATACTAATCTAAATTCCAGTCAATCGGTGACAGGCCCTTAGATTCGAAGTATTGATTTGC
TTCCTCGAAATGGCGACAGTTGAACGGACGTCGCGTGCAGGTAGAAGGATGGATGAACTCCAACTTTAAA
TGATTTTTAGGCACGCGGGGAATAAATCGTCTAGCTTCTTGGCCCCAAAGCATCAACACCGTAGATGACA
ACTTTTCTAGTAGCGATGAGATCACTCCCTTTGTAAATTTGTCCCAGCCGATGGTTGAGTGCGATCTAGG
AAAACCTTGTTCCACCGTCAAGTATTTGTTCAAAAGCAATACTCCCTTCCTGGCCCAAGATTCTAGACAC
CCGTGAGTTTCCTTTGGAATAGATGGGTAACAGTCGCGTAGAGCTTCCAAAATGCGGCGCAAGCTGGGCG
GTATACGGCACCCCCTAGGGACACTGAAAGCTAAGCCATGCGCGTGTCCTTCCGTGGGATACGGGTCTTG
CCCAACGATAATAACACGCACATCTTGCGGATTGCAGTATCTTGTCCATGCAAAAATATCTCCTTTAGGA
GGAAAGATTTTGCGGAATCTAGAACAGTAGGAGTACTGTAACAGCGTCTCACCGAAATCTTCATTTTCAA
TTAGAGGTTTCAGAATTGACTCCCATGATGATGGTATTGAAAACTCTATGGCCACGTCGGTCCACTCCGG
TACGAGCTTCCTCGCGGATGGTATAACCAATTGGACGGAAGTCATGCTCGGAGGGGGGAGAGTACGGCGT
TTTTTAGCAGCGTTGGGGTCGGTATCTCTTTGGTTGCTTTCAGTAGGAGAGCTAATGTCCAGCGGACGCT
TGAAGGGACTTTTTATGGGCTGCTCGCATAGTCCGGGTGATAGTGGTTGATGTTCGTTTCCGGCCATCTG
AAATTTATGGTGGTCCTTTTATACAACGAATCAACAAGTCCACGAGCCAAGACGTTAGCAGCCGCAAGCC
CGGTAGCTTCCAGCCGGTCTTGAAGAAGTCTAATCCCCAAGTACGGATTCACTTTTGCAGCGTACCCATT
CTTATAGTAGAAAACAACTTCCGTGTTTACGCACAGAGGTCTGTAAATAATCACAGTCAAATTTTCAAAG
TAGTCTCCCGATGGCAACGGCCCGAAAATTCGCGCACCGTTATCTCGCGAATTACTATCACATGGTGCGG
CCGCGGCAGAAACAGGATCACGAAAAATGCCAGGTATATCGACACTTGAACATAGCATAAGAGATGCTAT
TAGAAAAAAACAAGTCAACATTTTAAAAATCATTATCATATGGAGACCTAGGTCTCCGTTCACGATCCGT
GTTACGTCGACGAGCAGATGGAACTCTTTGCGCCCGACTTCTAAATGGATCGTCGTCAGAATCTGAGTCG
TCCGAATACAAATGTAGTGGTCTACAGGAACCAGTAGGTACTCTTTGTGGCGGACTTTTGAATGGATCGT
CGTCCGAATCAGAGTCGTCCCAATATAAATATGACGGCCTAGATGGAGTACTTCGGCTCTGTAGTCCTGA
AGGACCTTCACCTGCGTCATCGTTTCGCATGCGGAGGCTCCGATTCTGCATGGAATCGAGGTCTAAAATA
CCTCTGTCTGCGGCGACGGGGGATTCGGGTTCATTCTTTATCCCCCCATGACGAGTCTGTGCACCAGTCG
TATTTTGCCCTGCGCCATCATAATTAGACGAGGGGTGGTGGGGCGAAAGCTGTGGAGGGGAGCGTCTAGA
TGGCGAGGGGAGGGATGATAACCTGAGGTCTAGGGCCGTAGGAGATGGCGCAGAAAATAAAGGGGGTATA
GAGGACGGACGTGCGGGCGGTGGACTATGAGCGGGGCTATTTTCTATTTCATGCCGCACGACCCGTCGCA
CCCACGACATCTCATATACATCTCGCACTTCTGCTCTACTCGCAAGGCCATAGTTGCCTTCCCCCTCTTC
TGTCTCCGGTAACATAAACGTATCGGATACGGTGGTCGGCCGCTTTTTAACCATTGGATGTGCGAGCCCA
ACTGGCTTGACGTCCGTGCTGTCATGAGACTCGCCTTTGACAGGAAGGCGGATCGACACCATCCCGGCGG
AATTTTTAAAATATTTCAAATCCACCGTGAGTGGTCTGGTCAGCATGTCGGGCTGTATCGTCTCAGGAAT
GGCCATTCCTACCCTGTACTCTAGACCCGATGCACTCTGTACCGTCGGGGTTTGTTCGTACGCCAAAAAG
CTATCATATCCGCTAGGGTGATTCGTCGTAAACTCACGTATCCATCCATTGATCCTGGTTAGAAACGAGT
AAAATACTTCAAGCATCTGTTCTTCATCCTGGAATGACAATTCCACTGCGCGTAGTGCGGAATTTGCTCC
GCGCGTCACCAGATGACGCGCGTTTGAGATTTCTGGAGCTGAGGCGATTGCCATACTATAAACGACACTT
TTAGCCCTCAGTAGAGTAGAATACAGCGCCTCAAAATTTCTCCACGTTAGTTGACGAGTTACAAACGTTT
CAAGCATTATCGCCCTCAAAACGACTACAACGTCATCTATGGGGGATAGCCTTCCGGTATGGTGTCTTCG
AGTTACTGGTCTTTCCTGTTCCAAAATAGTCTGGATGGCTTGGCGTTCTCCATCGGTAATAGGGGTACCG
GCCTGCCGCCTGGTTTGAAGTAATCTCCTAACGCTTTCAACTGTTGTCGGAAAATAAGCTACAACAGAAA
CCAAACCATTAATCCTCGTACCTCAAAAAATCTTACACGCGTCATCCCACCCCACAAACCACTTACGCCT
CATCAAGACCCAGAAATCCATATAGTGCGCTTCATCGATGTCCGCCACGAATGCTATATTGAAATCTCCG
TTCATTGTCA

Protein Sequence : Show Sequence

>gi|148828637|ref|YP_182395.2| protein UL0 [Gallid herpesvirus 1]
MTMNGDFNIAFVADIDEAHYMDFWVLMRPYFPTTVESVRRLLQTRRQAGTPITDGERQAIQTILEQERPV
TRRHHTGRLSPIDDVVVVLRAIMLETFVTRQLTWRNFEALYSTLLRAKSVVYSMAIASAPEISNARHLVT
RGANSALRAVELSFQDEEQMLEVFYSFLTRINGWIREFTTNHPSGYDSFLAYEQTPTVQSASGLEYRVGM
AIPETIQPDMLTRPLTVDLKYFKNSAGMVSIRLPVKGESHDSTDVKPVGLAHPMVKKRPTTVSDTFMLPE
TEEGEGNYGLASRAEVRDVYEMSWVRRVVRHEIENSPAHSPPPARPSSIPPLFSAPSPTALDLRLSSLPS
PSRRSPPQLSPHHPSSNYDGAGQNTTGAQTRHGGIKNEPESPVAADRGILDLDSMQNRSLRMRNDDAGEG
PSGLQSRSTPSRPSYLYWDDSDSDDDPFKSPPQRVPTGSCRPLHLYSDDSDSDDDPFRSRAQRVPSARRR
NTDRERRPRSPYDNDF

Molecule Role : Virmugen
Molecule Role Annotation : A UL0 mutant in infectious laryngotracheitis virus (ILTV) was attenuated. Chickens immunized with UL0-negative ILTV were protected from clinical disease after subsequent infection with virulent ILTV. (Veits et al., 2003).
Related Vaccine(s): Gallid herpesvirus 1 UL0 mutant vaccine

3. UL23

Gene Name : UL23
Sequence Strain (Species/Organism) : Gallid herpesvirus 1
NCBI Gene ID : 3239035
NCBI Protein GI : 57790948
Locus Tag : GaHV1_gp17
Genbank Accession : L36139
Protein Accession : YP_182352
Taxonomy ID : 10386
Gene Starting Position : 29808
Gene Ending Position : 34666
Gene Strand (Orientation) : -
Protein Name : thymidine kinase
Protein pI : 5.45
Protein Weight : 37832.47
Protein Length : 364
Protein Note : 2.7.1.21

DNA Sequence : Show Sequence

>gi|57790931:29808-34666 Gallid herpesvirus 1, complete genome
GTTTATTGATGTTTATGTTTCGGGTAATTATCCAAACAGATCAGGCCGCGTCGCAATTCCCATGTTCTTC
CATGCTTCGGACACTGCTGTGCAAGCAGCCGTCTTAAAATCGCCTATCTCATCGGGGGTCCAATGAGAGT
GCAATAAAATGCCGAGTCTAGGTGGATCGTACGCGTGGCAAATTGCTGCGCACAGAAACTTGCCGCACAT
GGGAGTTTCTTCTAAAAGAGCGCGCCAGAAGGTAAAGCCTCGCCCAGTAAGCCAGCTAGTAGCTCTTGAT
GCCCATGTATCTACCGCTAGATATGAGCGTTCGTCCGATGCCAATACCCCCGAAACTGGGAAAAACGCGC
AAAGAATGTGAGTTCCTTTCGGAATCGAGGCTGTCTGCTGTGCGCGCGGTGGATGTATTTGGCTGTCGCC
AATCGCAACACAAAACGGGATACGGAAAGCCCATTCTTCGCGGCAAGTAATTATCGGCGAAGCTATCTGT
GATCGATACGCGCGTAACCCAGTAAATATCGCCGCTTGTAGTAGTCCGACGGGCGAGTGATGCGGGCTGT
CTCCGTGCTTTAAATCAAGGATTTCTCCGCTTAGAGTAAGTGATGGAGGACTTTGAAAGAACAAGGCCCC
GCTGAATGCGCGTACAGCAGCAACAACCGGAGAAAGTTCCAAATCCGAGATAAGTCCTAACATTAGAAAT
CCGACTCCATCTTTCCACTTGTCTGCAGACAACAGTTTAAAAGGCAAATACTTGGCTCCAAAAGTCATCG
GGTTTTTTGAATGCACGCTATCAGCTACGGCTTGCCATCTTTGGAAATTTTTTAAGGAAGCTCTTTTCAT
GGCAAGAAGGAGCTGCTCGACGCCAATCTCTAAATTAGAATTAGTTGTGTAGGGAGTTCTAGTCATGATA
ATTGCAGTAGAAGGAATTCCAGTGTCGTTGGAAAGGGATTCTTGCGGATCCTCAAATAATGGTGCTGCTA
CGAATCGGATTTGAGAGTTATCGGGCTGTTTTGAGTCATGGTCTTGAAACACATGAATAGTCAAATCCTT
GTTGCAATCCAGAAAAAACGATGCTGTGCCGTGCAGTACAGCTAGGCCATATTCTACCTTTTCTGTCCAT
AGTTTTCCACGATGTGTTCCTAATTCCATGTGAGACGAGTCTACAAAGTTTGCGAGGCAGTGAGTTACTG
CAAAACGACATTCGCGATTGCTGGTAGACAGTGCTTTGGAAGCCTGAGACTGCTTCTTTGACGGAGCCAT
AGCCAGTTATTCGTGCTGCCGCAAGTGCAGTTGAAAAGGGTTTGTAAGGAACAAGGATTAAAGTTCGCTC
TCCTCGTCAGACGGTAGCCGTACACGTCTAAATTGCGTTGCCGAGCTGAGGCAGAATTTATATATAGCCC
TGATGGCACTCCATAGAGCAACCAATCCAACAAGTGCCCCTACCACAATACCGATAATGTATGCCTTGTC
AAAAACTATTATGCTTCTGTCAAGTCCTAAAATTCGGACAGCAGTTCCGTGTGGTCCTAGAAGCGCGTAG
TTATAGAACTCGGGGTTCATAGACTTAAAAAATGCAATAGATGAGTTGGCACCGGCTAACAGTTCTGATT
GAACATATGCACTGTCTATAAAGAAAGCATCCTGGAACCCCCGTAGACCGTATTGAACTATCAGGTGGTT
GCAATATATGCAAGTTTTCGCTCGTCCATTCAAATGACCATCCTCCATTTTTGCTGTTCGCGCGATTTGC
GGTAGGGAGTTGCAACTTGAATTCACAAACGTTACAAACAAAGGGGCAGCTGTTGAGGTATCCGGAAGTC
TGTAGGTCATTCCAGTGCTCGGCGGGCTACTTGTCATCACCCAACTAGAATGCGCGGTAACAGGGAGGAA
AAATACGATGTGGTGTTCCAGGTCCCAGTTTAAACATTTCTTTACCTCTGGAAAAAACGCAGTTGCCAGG
ATCTGAGGGGTCAACAGAGCTTGTGGCGGTATCGGTGCACTATATGATGGCAATTCACCTTCAGATGATA
AATATATTTTGGTGTAAAAAAATCGAAATGGAGCAGATGCTGTCAGAATTATTTTCCATGGTGCACTTCT
GGAAATATCAAACCTTGTCGCAATTGCACAAGGAGAAAACCACTCTTCAATTTTCGATGCCTGTCCTTTC
TTGAAGCTCCCGTCTGGTTGGGAGTGAATCTCGTTGATATCGGCTAGCCGGTCGACAGAGCATAAAATTG
TCATGCGCCTGAGGAAGTTCCTTCCATGTTGTATAGAAAGTTGAGGAGCCCGCAAAATAATGTACGTAGA
AACTGCCAATAACTTTCTGTACGCGGGGGTCATTACCTTTTGTGTAATGATTTCTCTGTAACTGTTATCT
ATTATTTCAAGAGCCAGTTGGCTGGGGGTCACTCCATCGATGCTCGCCAGGAATGCATTTTGGGGATCTT
GTTCTGCAGTGATAAGCACCGCATTCATGATAGTAAGAAGCAGGTTATGATTTTTTATCATGGGTTCCCA
TGGCCTGTCGTGCATAATTTTAAGAATACTGGATGGGATAGAAGTAAGAGCACCACCGACATTCTGAATA
AAGCGAATATTCGTTGTATAGTTTACTGAATTCGCCCCCCCAAAACACACTGCGACTTGGTCTGCCCATG
TTTGCGTTTCTGCAATAAACTCGACGATCTCCGAATCAGTTACGTATGCATCGATCGTAAACTTTAGAAA
TCGAAACCACGCGGCCATAAACCGTGCTGCGAATCTTACAAAAAAAGACTCGTCTATGGTATTCGGGCAA
CCTGCGATTGCGACCTTCTGCAAAGTTAAAAAGTGTCCCGCGGATATTTGTTCGTCGAAGCTGGCCAGCT
CTAAGAAATCTTGAGCAGCGGTCGTTACTGCTGTAAACAAATTTGGCCGATAATTTGGTCCAGCTGCGAC
GTTGATGCGCACAGTTAACCCCTGATCTTCAGCCTTCAGCATATAGGGCCATGACTTCAGGGACGTTTCT
TTAACCTTCGCGCTGGTTGGTGTAATTATCATCTCTATTTGAGGAGAATGCTGGGTCGCGATTATAAGGG
TCGCCACATTCAGTCCGATGTTAATCTTAAGATCTATGTCACTAGTAACTGCGAGGCTAATATATCGTGT
AAACACAAATGGAAAAACAGTTTGTAAAGGATCACTGTAAATTACACGAATCGGAGCGATGATGCTCTCG
CCCCTATGAACTGAGTTATTTGGTGGAAAGTTGACGAGTTTCGCAGATGTAAGTGCATTCCCCTTGTATC
CTACCAAAAGTCGATGCTCAAGCTTTGGATCGTTGCTGAATTTTCGTGGTTGGATGGTAGCCTCGCTAGC
AGAACCCCATCTCGAGTAAGTATAGGCTACGTACAGTTGTCCAAGCAAATCTAGAGCGATCGATAAATTC
CTGTTAGCGCTTCCAGGAGGCCCAAAGATTAAGTTTCGATCGACAAGGCATAGTTGTGTGATGTGGCCGT
TTTCGACTGCTGTCGCTATCGCAATGAATTCTTTCGGAGGGGCCAAATTTTCACTGGTGGGGGGCACGAA
GACAATTTTTGCCTTTGCCTTGTCATCCCCTTTAGCTCTAAGTAATATCGCCAGAATGAGGAAAAATATT
TCTCTCGCGTGTCCCATCGGCGCGGGTTGCTGGTGAAGCCACGCTCTCTCGAGTAAGAATGAGTACAAAG
CAAGGAAATGAGTAAAGGACATACTCTTATTGTGAAGGATTATGGAAGCGGAACATTACGAACCCATATC
AGCATTGTAGCGCTCTACTGCGTCAATTAAGCTCGCGATCGCCGCTCGTTTGGTTGTAAGAGAACTTGCC
GTCTCGGCAAACTCTAAACATTTCAGTGCACATGCCTCCGGGGTGAGGCCATGTGCTGGTAAGTAAAAAA
TGCACAAGTTTTTCAATACCGTCGCGAGTTTTTGAACCACAAATGAAAGTACAGTTTTTAATCTTTCCCC
GTGAACATTTTGCTGATCAAAAGCTAATATTGCAAATAGCGTCTGGTCGATTGAAGGATTGATTTCTGGA
ACAGTAGCAGTTTCTAAGCACTCTCTCTTGAATTTTAAGAGCGTGGCACTTGTTAAGGCTTCTTGCTCCT
TCAACAATGCTGGATAAGATGTACGTTGGAGGTAGGTGGTAGTATTCATGAGGCAGATGTACGAGTTACG
TAATGCACACAAATACGTCCAGTCGACCGTTTCCCCTGGGCGCCCCCTTTGTATTATACGCCGTGCCTGT
TCGCAAATATCCAAATCCATTAAAACAATGTTGGAGTGTGTTGGAATGTCAGGAATTCCTGACAATAAAG
TTATTAAATCTCCAAATTCGAGCGCTCCGCTTAGATATTGTGCAACAGGGAAACACAAACATGCCGCGAG
TGGGTGCCTATCTACGAGGAATAAGACATCATCTGGTCCGGATGCAGCTGCGCTGTAGCAGCGTTCAGAT
CCCAAATAATTATAAATGACGTGGTTCATTATTCTTAGTGGGGCAGCAATAGTCATCTGAACTTCCGCTA
CGGCAACCGGAGACGCCTCCCGACATTCAAGTTTTCCGGAAGCCTTTAGGGTTTGTATTTCATTAATTTC
TTTGATCATATCCGCTCCGCCAAACCATTGGCGCCATGCTTGCATAGGTTCCGCTAAGTATAGACGGCAA
CCTCTCCAATTTGGCATTTGCATCAACGTCTTTGCAGTGACTGTTTTACCGACTCCGAATGGCCCGTCGA
CGTAAAGAAGTACAACGCGGCGAAAGTCGGAGTCATCGTTCTCGAACTCGGAGCAAAACTGCACACCACC
GGAAGTTTTCACGGCGCCAGCTACGGCCA

Protein Sequence : Show Sequence

>gi|57790948|ref|YP_182352.1| thymidine kinase [Gallid herpesvirus 1]
MAVAGAVKTSGGVQFCSEFENDDSDFRRVVLLYVDGPFGVGKTVTAKTLMQMPNWRGCRLYLAEPMQAWR
QWFGGADMIKEINEIQTLKASGKLECREASPVAVAEVQMTIAAPLRIMNHVIYNYLGSERCYSAAASGPD
DVLFLVDRHPLAACLCFPVAQYLSGALEFGDLITLLSGIPDIPTHSNIVLMDLDICEQARRIIQRGRPGE
TVDWTYLCALRNSYICLMNTTTYLQRTSYPALLKEQEALTSATLLKFKRECLETATVPEINPSIDQTLFA
ILAFDQQNVHGERLKTVLSFVVQKLATVLKNLCIFYLPAHGLTPEACALKCLEFAETASSLTTKRAAIAS
LIDAVERYNADMGS

Molecule Role : Virmugen
Molecule Role Annotation : A thymidine-kinase mutant is attenuated in chickens and induced protection from challenge with wild type GHV-1 (Han et al., 2002).
Related Vaccine(s): Gallid herpesvirus 1 UL23 mutant vaccine

4. UL27

Gene Name : UL27
Sequence Strain (Species/Organism) : Gallid alphaherpesvirus 1
NCBI Gene ID : 3239023
NCBI Protein GI : 57790952
Locus Tag : GaHV1_gp21
Protein Accession : YP_182356
Taxonomy ID : 10386
Gene Starting Position : 39070
Gene Ending Position : 41746
Gene Strand (Orientation) : -
Protein Name : envelope glycoprotein B
Protein pI : 6.82
Protein Weight : 94517.88
Protein Length : 883

DNA Sequence : Show Sequence

>NC_006623.1:39070-41746 Gallid herpesvirus 1, complete genome
TTTTATTAAATAAACTGCATTTAAACTTATTCGTCTTCGCTTTCTTCTGCCCACGAAGGGTATTCGAGCA
GGGGGTAGTCATCTTCCTTGGTTCGGGGTTTTCTGCGAAATAGGTTTCTCATATTTTTAGCAAAAGCAGA
AAACCGCGCTTTCTGTCTTCGAGCTTTCTGTTCATCCTTGTGTAGAAGCTCAAGGCCTTTCAGAACTCTG
GTGGCAAGTATCCTGTCGTCCTCATCACTATCCTCCTCAACCTCCTCCTCATCCTTGTAGTAACGTCTAG
AGGGTCGTGGAGGAGTTCCGGCCGGGGGAACTGCGCCTGGGAACAGAACCTGAACTGGGTTTGATTTCAG
GTTCATTACATATTTGAACGCCAGCAGTCCTAAAATAATGCTCACCACCACCGCTATCCCAATTCCGAGT
GCGGCGAACGGGTTAGAAAGAAATGAGGCGATGCCAGATACTGTAGAAATTACTGCTGCCGCGGCTGTCA
TCACTACAGTTCCCAATGCCTTTCCTACTTCTCCAAGAGTGTTGCCAAAAAAATCTGCTATTGCTCTAAA
GATTGCATCTCCCCTATCTCCACGTATTACAGTGTCAATGTCTCTGAACCTTTTGTTATAAATATTTTGA
TATCTGACCACATCATCATAGTTTAAAGTCCCAGTATCTCTGAGTTCCTCGCGAGTGTATACTTCGACGG
GCACAAAGTCCAAATCTTCTAGTATAGTGGCGTTCAAGTTGATGAATATGCTCACTTCTTCGATCTCGGA
AGCATTTACTTGTCTAACAAATGTATAGTCTTCGTAAAGAAGGTAGTTTTCTCCCAACAGAAAGTACCGT
CTGTGATTGATCATGCATGGTTCTATCAAATTCCGCCCTTGTAAAATTTCATTATGTTCTCCGAGTTGGC
CTAATATGTCAAGATTGTGGTTTTCTGTTGAGTTCGCAGAAAACTGTGACTCAGGGGAGGACGAATACCT
GAAAATAAGTACTGGTCTGGTATAGCACATGGTTGGGTCCCCTGGCATGCGCATGGAATCCTGCATCCTA
ATATTTTCGATTGGAATTTCTATACATTTTGATACCGCTACGATGTCTCCCAATAGCCTGGCGCTTACAG
GTTGTCCGAACAAAGATGTCATCAGTGAGTTCGGGTTTAGTTTCTTCATCTCATGCCATACAATCAGTTG
GCGGTTCTGAAGCTCACACCACGCTTCCAACAAATTTCCGATAAGCTCGTTAACATGGGCTTGGATTTTG
TCGTATGCAAATTGTAACATGGCAAATGTTGCAGAACTGTGGGTAGTAATTCTATCACCCTGAGGTCCAG
ACTGCAGCGACGCCGTGCGGCGACCTGCGGCTTGGCGACGCTCTCTCCCTCTCGGGAGATGATTTTGTCT
TTGTGCCTCTTCTAGGTACATTTCAGCCAAGCCATGGCTCATGAGTTTCTGAAATGCGATCAGAAATCCG
CCACTACCGAGATAGTATTCGATGTCCCCAGAACGGACATGAGTCGAACTATACTTTCTGGCAAAGATGC
CATCTATGGCCTCCGTGGCTATGGTAGGAACGCATTCAGCCAAATGAAGCCTGCTGATGTTAAAAGGTTG
TTTTCCGGACGAGAACGTCATAGTCATATCTTTAAGTGAAAAGTGGTAACTGTTTTTGTACGAAACACGA
ACTGCTTCCGGGACTTCAATCCATTTACTGAGAGTACATACAGATTCCTTTTCTTCCATTGCATCCCAGC
CTATAGTGAATTGTTCATCTGTTAGAAAGTTTCTTTTTTTAGGGGGTCTTATTTGTCCGGTTTCCAAATC
CCTGACTTGATAATTTGCGATTTCGAGAAATCTATAGTCTCTGTAGACACTGTGACGCCTTGGTCCGGTC
GTGTTTTTGGTATAAAAAGGAGAAATTTCTACTGTATCACCTGTCGCCATTCCAAAGTAATCATACGGGT
ATACAGATCTAGCCTGTAGATATTCCACAACACAGTCGACCGATGTAGACGTTCGGTATCCCAGGGTTTG
ATGTCGCTTAGTAAAGTTAGTTGTATGAAACGCCTTGGAGACAGTTGATCTTAACAGTGATGGAACCAGG
GGCAATTTTTTTTCTGCTTCATCATTGTCGTAAGCTTCAAAAAACATGAAATTTTTATGATACGTTGCTT
TGGATGAGCATTCTCCCGATCGATCAATCCTGACAATTTCATGATAGTCTATTGGAACCCTGGTCACGTA
CTCATTTGTTATTTGGGGTCTTGAGAATAATGCCCACGTAGTAACTGTGGTTATATGTTTATAGTATAGA
GTCACATTAAAGACGTACGGGGCAATGTTTTGCTTGAAGACTACGGCAATTCCTTCAGTCATGTTAGTCG
AATCGGCATGTCGGTGACAATGTCGTGGCTGGGCAAGCCTCGCAACACTGGCGCCAGTTGGAGATGAGCA
CGCAAAAATTCTGTTTCTAATCTGGGGTTCTTTCGGTGCCCCCGACCCAATGGCTTCGGAGAAAGAGATT
TTTCCAACATCCATGCTGGCTGTATCACGAGGGTCTATTATTCCAGCAATTCCGTGTGAATCTTGAGATA
GGGTAGATGGGATCAGGATTGCCACGCACACGCAGATCAGCATTTTCAAGCTAGCCATGTCAATGTTCAC
GGCGATGTAGGATTGCA

Protein Sequence : Show Sequence

>YP_182356.1 envelope glycoprotein B [Gallid alphaherpesvirus 1]
MQSYIAVNIDMASLKMLICVCVAILIPSTLSQDSHGIAGIIDPRDTASMDVGKISFSEAIGSGAPKEPQI
RNRIFACSSPTGASVARLAQPRHCHRHADSTNMTEGIAVVFKQNIAPYVFNVTLYYKHITTVTTWALFSR
PQITNEYVTRVPIDYHEIVRIDRSGECSSKATYHKNFMFFEAYDNDEAEKKLPLVPSLLRSTVSKAFHTT
NFTKRHQTLGYRTSTSVDCVVEYLQARSVYPYDYFGMATGDTVEISPFYTKNTTGPRRHSVYRDYRFLEI
ANYQVRDLETGQIRPPKKRNFLTDEQFTIGWDAMEEKESVCTLSKWIEVPEAVRVSYKNSYHFSLKDMTM
TFSSGKQPFNISRLHLAECVPTIATEAIDGIFARKYSSTHVRSGDIEYYLGSGGFLIAFQKLMSHGLAEM
YLEEAQRQNHLPRGRERRQAAGRRTASLQSGPQGDRITTHSSATFAMLQFAYDKIQAHVNELIGNLLEAW
CELQNRQLIVWHEMKKLNPNSLMTSLFGQPVSARLLGDIVAVSKCIEIPIENIRMQDSMRMPGDPTMCYT
RPVLIFRYSSSPESQFSANSTENHNLDILGQLGEHNEILQGRNLIEPCMINHRRYFLLGENYLLYEDYTF
VRQVNASEIEEVSIFINLNATILEDLDFVPVEVYTREELRDTGTLNYDDVVRYQNIYNKRFRDIDTVIRG
DRGDAIFRAIADFFGNTLGEVGKALGTVVMTAAAAVISTVSGIASFLSNPFAALGIGIAVVVSIILGLLA
FKYVMNLKSNPVQVLFPGAVPPAGTPPRPSRRYYKDEEEVEEDSDEDDRILATRVLKGLELLHKDEQKAR
RQKARFSAFAKNMRNLFRRKPRTKEDDYPLLEYPSWAEESEDE

Molecule Role : Protective antigen

5. UL47

Gene Name : UL47
Sequence Strain (Species/Organism) : Gallid herpesvirus 1
NCBI Gene ID : 3239064
NCBI Protein GI : 57790998
Locus Tag : GaHV1_gp67
Genbank Accession : EU104855
Protein Accession : YP_182402
Taxonomy ID : 10386
Gene Starting Position : 126615
Gene Ending Position : 129614
Gene Strand (Orientation) : +
Protein Name : tegument protein VP13/14
Protein pI : 9.89
Protein Weight : 65621.89
Protein Length : 623

DNA Sequence : Show Sequence

>gi|57790931:126615-129614 Gallid herpesvirus 1, complete genome
AATGACCTTGCCCCATCGATTAACGAAAAGACCTTTCGCGCGTCGATTCTGCTCGGTCTTTGTGATACAT
TATAGTGAGACTAAACTCGACCGATATAACAAGACAATGTTACTCTATAGACCGGACTCAACCATGCGGC
ATAGCGGAGGCGACGCAAATCACAGAGGGATAAGGCCGAGGCGGAAATCTATTGGAGCGTTTAGCGCGCG
CGAAAAGACTGGAAAACGAAATGCGCTGACGGAAAGCAGCTCCTCCTCCGACATGCTAGATCCGTTTTCC
ACGGATAAGGAATTTGGCGGTAAGTGGACGGTAGACGGACCTGCCGACATTACTGCCGAGGTCCTTTCTC
AGGCATGGGACGTTCTCCAATTAGTGAAGCATGAAGATGCGGAGGAGGAGAGAGTGACTTATGAGTCCAA
ACCGACCCCGATACAGCCGTTCAATGCCTGGCCGGACGGGCCGAGTTGGAACGCGCAGGATTTTACTCGA
GCGCCAATAGTTTATCCCTCTGCGGAGGTATTGGACGCAGAGGCGTTGAAAGTAGGGGCATTCGTTAGCC
GAGTTTTACAATGTGTACCGTTCACGCGATCAAAGAAAAGCGTTACGGTGCGGGATGCGCAGTCGTTTTT
GGGGGACTCGTTCTGGAGAATAATGCAGAACGTTTACACGGTTGTCTTACGACAGCACATAACTCGACTC
AGGCACCCTTCCAGCAAAAGCATTGTTAACTGCAACGACCCTCTATGGTACGCCTACGCGAATCAATTTC
ACTGGAGAGGAATGCGCGTGCCGTCGCTTAAATTAGCCTCTCCCCCGGAGGAGAATATTCAACACGGCCC
AATGGCCGCCGTTTTTAGAAACGCGGGGGCTGGTCTGTTCCTGTGGCCTGCCATGCGCGCAGCCTTTGAA
GAGCGCGACAAGCGACTGTTAAGAGCATGCCTGTCTTCACTCGATATCATGGACGCAGCCGTCCTCGCGT
CGTTTCCATTTTACTGGCGCGGCGTCCAAGACACCTCGCGCTTCGAGCCTGCGCTGGGCTGTTTGTCAGA
GTACTTTGCACTAGTGGTGTTACTGGCCGAGACGGTCTTAGCGACCATGTTCGACCACGCACTGGTATTC
ATGAGGGCGCTGGCAGACGGCAATTTCGATGACTATGACGAAACTAGATATATAGACCCCGTTAAAAACG
AGTACCTGAACGGAGCCGAGGGTACTCTGTTACGGGGCATAGTGGCCTCCAACACCGCTCTGGCGGTGGT
TTGCGCAAACACCTATTCGACGATAAGAAAACTCCCGTCCGTGGCAACTAGCGCGTGCAATGTTGCCTAC
AGGACCGAAACGCTGAAAGCGAGGCGCCCTGGCATGAGCGACATATACCGGATATTACAAAAAGAGTTTT
TCTTTTACATTGCGTGGCTCCAGAGGGTTGCAACACACGCAAATTTCTGTTTAAACATTCTGAAGAGAAG
CGTGGATACGGGGGCCCCGCCATTTTTGTTCAGGGCCAGCTCGGAGAAGCGGCTGCAGCAGTTAAATAAA
ATGCTCTGCCCCCTTCTCGTGCCGATTCAATATGAAGACTTTTCGAAGGCCATGGGGTCTGAGCTCAAGA
GGGAAAAGTTAGAGACATTCGTTAAAGCTATTTCCAGCGACAGGGACCCGAGGGGGTCCTTAAGATTTCT
CATTTCGGACCATGCAAGGGAAATTATTGCAGACGGAGTACGGTTTAAGCCGGTGATAGACGAGCCGGTT
CGGGCTTCAGTTGCGCTGAGTACCGCTGCCGCTGGGAAAGTGAAAGCGCGACGCTTAACCTCAGTTCGCG
CGCCCGTACCGGGCGCAGGCGCCGTTTCCGCGCGCCGGAAATCGGAAATATGATAAAAATGCTTGGCATT
TGCGGGCGAAGAGGCGTGATCTGAAGGGCTCCACAATGACGTAACTGAGCTACGCATCCCTATAAAGTGT
ACCCGCTGACCGCTAGCCCATACAGTGTTACAGGAGGGGAGAGAGACAACTTCAGCTCGAAGTCTGAAGA
GACATCATGAGCGGCTTCAGTAACATAGGATCGATTGCCACCGTTTCCCTAGTATGCTCGCTTTTGTGCG
CATCTGTATTAGGGGCGCCGGTACTGGACGGGCTCGAGTCGAGCCCTTTCCCGTTCGGGGGCAAAATTAT
AGCCCAGGCGTGCAACCGCACCACGATTGAGGTGACGGTCCCGTGGAGCGACTACTCTGGTCGCACCGAA
GGAGTGTCAGTCGAGGTGAAATGGTTCTACGGGAATAGTAATCCCGAAAGCTTCGTGTTCGGGGTGGATA
GCGAAACGGGCAGTGGACACGAGGACCTGTCTACGTGCTGGGCTCTAATCCATAATCTGAACGCGTCTGT
GTGCAGGGCGTCTGACGCCGGGATACCTGATTTCGACAAGCAGTGCGAAAAAGTGCAGAGAAGACTGCGC
TCCGGGGTGGAACTTGGTAGTTACGTGTCTGGCAATGGATCCCTGGTGCTGTACCCAGGGATGTACGATG
CCGGCATCTACGCCTACCAGCTCTCAGTGGGTGGGAAGGGATATACCGGGTCTGTTTATCTAGACGTCGG
ACCAAACCCCGGATGCCACGACCAGTATGGGTACACCTATTACAGCCTGGCCGACGAGGCGTCAGACTTA
TCATCTTATGACGTAGCCTCGCCCGAACTCGACGGTCCTATGGAGGAAGATTATTCCAATTGTCTAGACA
TGCCCCCGCTACGCCCATGGACAACCGTTTGTTCGCATGACGTCGAGGAGCAGGAAAACGCCACGGACGA
GCTTTACCTATGGGACGAGGAATGCGCCGGTCCGCTGGACGAGTACGTCGACGAAAGGTCAGAGACGATG
CCCAGGATGGTTGTCTTTTCACCGCCCTCTACGCTCCAGCAGTAGCCACCCGAGAGTGTTTTTTGTGAGC
GCCCACGCAACATACCTAACTGCTTCATTTCTGATCAATTATTGCGTATTGAATAAATAA

Protein Sequence : Show Sequence

>gi|57790998|ref|YP_182402.1| tegument protein VP13/14 [Gallid herpesvirus 1]
MTLPHRLTKRPFARRFCSVFVIHYSETKLDRYNKTMLLYRPDSTMRHSGGDANHRGIRPRRKSIGAFSAR
EKTGKRNALTESSSSSDMLDPFSTDKEFGGKWTVDGPADITAEVLSQAWDVLQLVKHEDAEEERVTYESK
PTPIQPFNAWPDGPSWNAQDFTRAPIVYPSAEVLDAEALKVGAFVSRVLQCVPFTRSKKSVTVRDAQSFL
GDSFWRIMQNVYTVVLRQHITRLRHPSSKSIVNCNDPLWYAYANQFHWRGMRVPSLKLASPPEENIQHGP
MAAVFRNAGAGLFLWPAMRAAFEERDKRLLRACLSSLDIMDAAVLASFPFYWRGVQDTSRFEPALGCLSE
YFALVVLLAETVLATMFDHALVFMRALADGNFDDYDETRYIDPVKNEYLNGAEGTLLRGIVASNTALAVV
CANTYSTIRKLPSVATSACNVAYRTETLKARRPGMSDIYRILQKEFFFYIAWLQRVATHANFCLNILKRS
VDTGAPPFLFRASSEKRLQQLNKMLCPLLVPIQYEDFSKAMGSELKREKLETFVKAISSDRDPRGSLRFL
ISDHAREIIADGVRFKPVIDEPVRASVALSTAAAGKVKARRLTSVRAPVPGAGAVSARRKSEI

Molecule Role : Virmugen
Molecule Role Annotation : A UL47 mutant in avian infectious laryngotracheitis virus is attenuated and provides protection in chicken against subsequent challenge infection with virulent ILTV (Helferich et al., 2007).
Related Vaccine(s): Gallid herpesvirus 1 UL47 mutant vaccine

III. Vaccine Information

1. Avian Encephalomyelitis-Fowl Pox-Laryngotracheitis Live Virus, Live Fowl Pox Vector Vaccine (USDA: 10S1.R0)

a. Manufacturer:

Biomune Company

b. Vaccine Ontology ID:

VO_0002038

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

2. Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.01)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0001671

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

3. Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.02)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0001672

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

4. Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.11)

a. Manufacturer:

Wyeth, Lohmann Animal Health International, Merial, Inc.

b. Vaccine Ontology ID:

VO_0001673

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

5. Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.13)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0001674

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

6. Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.14)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0001675

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

7. Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.15)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0001676

c. Type:

Live, attenuated vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

8. Fowl Laryngotracheitis-Marek's Disease Serotype 3, Live Marek's Disease Vector Vaccine (USDA: 16J1.R1)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0002141

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

9. Fowl Laryngotracheitis-Marek's Disease Serotypes 2 & 3, Modified Live & Live Marek's Disease Vector Vaccine (USDA: 16J1.R2)

a. Manufacturer:

Intervet Inc.

b. Vaccine Ontology ID:

VO_0002142

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

10. Fowl Pox-Laryngotracheitis Live Fowl Pox Vector Vaccine (USDA: 1F31.R0)

a. Manufacturer:

Biomune Company

b. Vaccine Ontology ID:

VO_0002202

c. Type:

Live vaccine

d. Status:

Licensed

e. Location Licensed:

USA

f. Host Species for Licensed Use:

Chicken

11. Gallid herpesvirus 1 UL0 mutant vaccine

a. Vaccine Ontology ID:

VO_0002966

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Chicken

e. Gene Engineering of UL0

Type: Gene mutation
Description: This UL0 mutant is from Gallid herpesvirus 1 (Veits et al., 2003).
Detailed Gene Information: Click here.

f. Immunization Route

Eye drop

g. Chicken Response

Persistence: A UL0 mutant is attenuated in chickens (Veits et al., 2003).
Efficacy: A UL0 mutant induces significant protection in chickens from challenge with wild type gallid herpesvirus 1 (Veits et al., 2003).

12. Gallid herpesvirus 1 UL23 mutant vaccine

a. Vaccine Ontology ID:

VO_0002967

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Chicken

e. Gene Engineering of UL23

Type: Gene mutation
Description: This UL23 mutant is from Gallid herpesvirus 1 (Han et al., 2002).
Detailed Gene Information: Click here.

f. Immunization Route

Intraocular immunization

g. Chicken Response

Persistence: A UL23 mutant is attenuated in chickens (Han et al., 2002).
Efficacy: A UL23 mutant induces significant protection in chickens from challenge with wild type GHV-1 (Han et al., 2002).

13. Gallid herpesvirus 1 UL47 mutant vaccine

a. Vaccine Ontology ID:

VO_0002968

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Chicken

e. Gene Engineering of UL47

Type: Gene mutation
Description: This UL47 mutant is from Gallid herpesvirus 1 (Helferich et al., 2007).
Detailed Gene Information: Click here.

f. Immunization Route

Intratracheal immunization

g. Chicken Response

Persistence: A UL47 mutant is attenuated in chickens (Helferich et al., 2007).
Efficacy: A UL47 mutant induces significant protection in chickens from challenge with wild type GHV-1 (Helferich et al., 2007).

14. Laryngotracheitis DNA Vaccine encoding gB

a. Vaccine Ontology ID:

VO_0004565

b. Type:

DNA vaccine

c. Status:

Research

d. Gene Engineering of gB

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

e. Vector:

pcDNA3.1(+) (Chen et al., 2011)

f. Immunization Route

Intramuscular injection (i.m.)

g. Baboon Response

Vaccination Protocol: At 21 days of age, the chickens were randomly divided into four groups of 20 chickens each. Chickens in group 1 were administered 150 μg plasmid pgB alone by intramuscular injection into the quadriceps muscle. Chickens in group 2 were immunized with 150 μg plasmid pgB plus 150 μg plasmid pIL-18. Chickens in group 3, which served as a negative-control group, were immunized with 150 μg plasmid pcDNA3.1(+). Chickens in group 4 were immunized with 0.5 ml sterile PBS alone as a blank control (Chen et al., 2011).
Vaccine Immune Response Type: VO_0003057
Immune Response: Compared with the chickens in the pcDNA3.1(+) and PBS group, the chickens in the pgB with or without pIL-18 groups showed significantly increased levels of anti-ILTV antibodies with subsequent vaccination. In addition, an enhanced T-cell proliferative response to gB was clearly observed in the groups immunized with pgB alone or with pgB plus pIL-18 (Chen et al., 2011).
Challenge Protocol: At 15 days after the booster immunization, all of the chickens were challenged intratracheally with 150 EID50 virulent ILTV CG strain (Chen et al., 2011).
Efficacy: The protection rates in the pgB and pgB plus pIL-18 groups were 60 and 80 %, respectively (Chen et al., 2011).

IV. References

1. Chen et al., 2011: Chen HY, Zhang HY, Li XS, Cui BA, Wang SJ, Geng JW, Li K. Interleukin-18-mediated enhancement of the protective effect of an infectious laryngotracheitis virus glycoprotein B plasmid DNA vaccine in chickens. Journal of medical microbiology. 2011; 60(Pt 1); 110-116. [PubMed: 20829398].

2. Fuchs et al., 2007: Fuchs W, Veits J, Helferich D, Granzow H, Teifke JP, Mettenleiter TC. Molecular biology of avian infectious laryngotracheitis virus. Veterinary research. 2007; 38(2); 261-279. [PubMed: 17296156].

3. Han et al., 2002: Han MG, Kweon CH, Mo IP, Kim SJ. Pathogenicity and vaccine efficacy of a thymidine kinase gene deleted infectious laryngotracheitis virus expressing the green fluorescent protein gene. Archives of virology. 2002; 147(5); 1017-1031. [PubMed: 12021870].

4. Helferich et al., 2007: Helferich D, Veits J, Teifke JP, Mettenleiter TC, Fuchs W. The UL47 gene of avian infectious laryngotracheitis virus is not essential for in vitro replication but is relevant for virulence in chickens. The Journal of general virology. 2007; 88(Pt 3); 732-742. [PubMed: 17325345].

5. Veits et al., 2003: Veits J, Lüschow D, Kindermann K, Werner O, Teifke JP, Mettenleiter TC, Fuchs W. Deletion of the non-essential UL0 gene of infectious laryngotracheitis (ILT) virus leads to attenuation in chickens, and UL0 mutants expressing influenza virus haemagglutinin (H7) protect against ILT and fowl plague. The Journal of general virology. 2003; 84(Pt 12); 3343-3352. [PubMed: 14645915].