|
Staphylococcus aureus |
Table of Contents |
- General Information
- NCBI Taxonomy ID
- Disease
- Introduction
- Microbial Pathogenesis
- Host Ranges and Animal Models
- Host Protective Immunity
- Vaccine Related Pathogen Genes
- ClfA
(Protective antigen)
- clfB
(Protective antigen)
- cna
(Protective antigen)
- Csa1A
(Protective antigen)
- efb
(Protective antigen)
- entA_1
(Protective antigen)
- esxA
(Protective antigen)
- esxB
(Protective antigen)
- Fhud2
(Protective antigen)
- fnbA
(Protective antigen)
- FnbpA
(Protective antigen)
- gapC
(Protective antigen)
- gst
(Protective antigen)
- hla
(Protective antigen)
- hlgB
(Protective antigen)
- isdB
(Protective antigen)
- IsdH
(Protective antigen)
- lukF
(Protective antigen)
- lukS
(Protective antigen)
- MecA
(Protective antigen)
- mntC
(Protective antigen)
- sasA
(Protective antigen)
- sdrD
(Protective antigen)
- seb
(Protective antigen)
- spa
(Protective antigen)
- staphylococcal enterotoxin A
(Protective antigen)
- traP
(Protective antigen)
- TSST-1
(Protective antigen)
- aroA
(Virmugen)
- Vaccine Related Host Genes
- Ifng (Interferon gamma)
- Il4 (interleukin 4)
- Vaccine Information
- S. aureus ClfA Protein Vaccine
- S. aureus CNA Protein Vaccine
- S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna
- S. aureus DNA Vaccine encoding PBP2a Protein
- S. aureus DNA vaccine pClfaSrtD13
- S. aureus FnbA Protein Vaccine
- S. aureus IsdB subunit vaccine (V710)
- Staphylococcus aureus aroA mutant vaccine
- References
|
I. General Information |
1. NCBI Taxonomy ID: |
1280 |
2. Disease: |
Staph infections |
3. Introduction |
Staphylococcus aureus is a facultatively anaerobic, gram-positive coccus and is the most common cause of staph infections. It is frequently part of the skin flora found in the nose and on skin. S. aureus can cause a range of illnesses from minor skin infections, such as pimples, impetigo, boils (furuncles), cellulitis folliculitis, carbuncles, scalded skin syndrome, and abscesses, to life-threatening diseases such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome (TSS), chest pain, bacteremia, and sepsis. Its incidence is from skin, soft tissue, respiratory, bone, joint, endovascular to wound infections. It is still one of the five most common causes of nosocomial infections, often causing postsurgical wound infections (Wiki: S. aureus). |
4. Microbial Pathogenesis |
The initial critical event in most staphylococcal infections is the ability of bacteria to adhere to components within host tissues (Nilsson et al., 1998). |
5. Host Ranges and Animal Models |
S. aureus can survive on domesticated animals such as dogs, cats, and horses, and can cause bumblefoot in chickens. It is also a common commensal of human skin (Wiki: S. aureus). Mice are used as an animal model of infection (Senna et al., 2003). |
6. Host Protective Immunity |
In general, protection against S. aureus infection is attributed to intact epithelial and mucosal barriers and normal cellular and humoral responses (Nilsson et al., 1998). |
II. Vaccine Related Pathogen Genes |
1. aroA |
-
Gene Name :
aroA
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus subsp. aureus ED98
-
NCBI Gene ID :
8614197
-
NCBI Protein GI :
269203089
-
Locus Tag :
SAAV_1451
-
Genbank Accession :
CP001781
-
Protein Accession :
YP_003282358
-
Taxonomy ID :
681288
-
Gene Starting Position :
1517365
-
Gene Ending Position :
1518663
-
Gene Strand (Orientation) :
-
-
Protein Name :
3-phosphoshikimate 1-carboxyvinyltransferase
-
Protein pI :
4.84
-
Protein Weight :
44289.51
-
Protein Length :
432
-
DNA Sequence : Show Sequence
>gi|269201690:1517365-1518663 Staphylococcus aureus subsp. aureus ED98, complete genome
ATTATCCCTCATTTTCTAAAAGCTTTAGTTTTGGTAAAAATCCTGGAAATGATACATTTACAGCATCAAA
TTGTTTGATTTTGACAGGCTCGCTTGAAAGTAGAGAAGCAACTGCAAGCATCATTCCTATTCGATGATCA
GTTAAACTATCAACTGTTGCATTTGTTTTAAATTCTGACGGATGAATAATCAATCCATCATTAGTTGGTT
GTAATTCAAACCCTAACAAGTTTAACATATCAGCCGTTGTATCAATTCTATTTGTTTCTTTTACTTTTAA
TTCCTCGGCATCTTTAATTGTACTCGTGCCAACTGCTTGTGTACAAAGTAATGCTATTACAGGCAGTTCA
TCAATTGCTTTTGGAACTAATTCTCCTTCGATTGTTATTGGTTGAAGCATTGGTGTGTATTGAATACGAA
TAGAAGCAGTAGGTTCAGCACCAGTTGTTTGATTGAAAAGTTGGATATTACCGCCCATTTTTTCAACAAT
ATCAATAATACCTGAACGTGTTGGATTGATTCCAACATTATGAATTGTTACATCACTTTCTGGTGTGATA
AGTGCTGCAACAATAAAGAACGCTGCAGATGAAATATCGCCAGGAACATGAAAATCTGCAGGTTTAATAT
GTTGAATGGCATCAGGGGTTGTAGTTATTGATAACCTTTCTGCTTCAATTGGAATATTAAAATGTCTGAA
CATCGTCTCAGTATGATTTCGACTTACATCTAATTCCTTTATTACCGTTGTATCATTTGAAAACAAACTT
GCAAATAAAATGGCACTTTTTACTTGTGCACTTGCAACTTCCATTTGATAATTTATACCTTTTATGACAG
ATGGCTTAATAATTAATGGTGTATAATTATCTTCAATACCTTCAATATTCGCATCCATAAGTTTCAATGG
TCTCAAGACACGATCCATTGGCCTTTTACCAATTGAAACATCGCCAGACAAAACACTTTCAATACCTAAA
CCACTTAACAAACCAGCTAACAATCGAGTCGTTGTGCCAGAGTTTCCAGTATATAAAACTTGATGAGGTG
TTTTAAAAGCTTTATATCCAGGTGAATTCACAACCAATTTATCTTCATCTTCTTTAATATCTACGCCTAA
TAATCGGAATATATCCATTGTACGACGACAATCTTCACCAAGTAATGGTTTATAAATATTGGAAGTTCCT
TCAGCTAAAGATGCTAACATGATTGCTCGATGTGTCATCGACTTATCTCCAGGCACTTCTATTTCACCTT
TAAGTGGCCCTGAAATATCGATAATTTGTTCACTTACCA
-
Protein Sequence : Show Sequence
>gi|269203089|ref|YP_003282358.1| 3-phosphoshikimate 1-carboxyvinyltransferase [Staphylococcus aureus subsp. aureus ED98]
MVSEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGTSNIYKPLLGEDCRRTMDIFRLLGVDIKEDED
KLVVNSPGYKAFKTPHQVLYTGNSGTTTRLLAGLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIE
GIEDNYTPLIIKPSVIKGINYQMEVASAQVKSAILFASLFSNDTTVIKELDVSRNHTETMFRHFNIPIEA
ERLSITTTPDAIQHIKPADFHVPGDISSAAFFIVAALITPESDVTIHNVGINPTRSGIIDIVEKMGGNIQ
LFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTSTIKDAEELKVKETNRID
TTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIGMMLAVASLLSSEPVKIKQFDAVNVSFPG
FLPKLKLLENEG
-
Molecule Role :
Virmugen
-
Molecule Role Annotation :
An aroA mutant is attenuated in mice and induced significant protection from challenge with wild type S. aureus (Buzzola et al., 2006).
- Related Vaccine(s):
Staphylococcus aureus aroA mutant vaccine
|
2. ClfA |
-
Gene Name :
ClfA
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus subsp. aureus str. Newman
-
VO ID :
VO_0012390
-
NCBI Gene ID :
5330438
-
NCBI Protein GI :
151220968
-
Locus Tag :
NWMN_0756
-
Genbank Accession :
AP009351
-
Protein Accession :
YP_001331790
-
3D structure: PDB ID :
1N67
-
Taxonomy ID :
426430
-
Gene Starting Position :
849756
-
Gene Ending Position :
852557
-
Gene Strand (Orientation) :
?
-
Protein Name :
clumping factor A
-
Protein pI :
3.44
-
Protein Weight :
91994.79
-
Protein Length :
933
-
Protein Note :
fibrinogen-binding protein A
-
DNA Sequence : Show Sequence
>NC_009641.1:849756-852557 Staphylococcus aureus subsp. aureus str. Newman DNA, complete genome
AATGAATATGAAGAAAAAAGAAAAACACGCAATTCGGAAAAAATCGATTGGCGTGGCTTCAGTGCTTGTA
GGTACGTTAATCGGTTTTGGACTACTCAGCAGTAAAGAAGCAGATGCAAGTGAAAATAGTGTTACGCAAT
CTGATAGCGCAAGTAACGAAAGCAAAAGTAATGATTCAAGTAGCGTTAGTGCTGCACCTAAAACAGACGA
CACAAACGTGAGTGATACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCA
GCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAG
CTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGA
ATTAGTGAATCAAACAAGTAATGAAACGACTTTTAATGATACTAATACAGTATCATCTGTAAATTCACCT
CAAAATTCTACAAATGCGGAAAATGTTTCAACAACGCAAGATACTTCAACTGAAGCAACACCTTCAAACA
ATGAATCAGCTCCACAGAGTACAGATGCAAGTAATAAAGATGTAGTTAATCAAGCGGTTAATACAAGTGC
GCCTAGAATGAGAGCATTTAGTTTAGCGGCAGTAGCTGCAGATGCACCGGCAGCTGGCACAGATATTACG
AATCAGTTGACGAATGTGACAGTTGGTATTGACTCTGGTACGACTGTGTATCCGCACCAAGCAGGTTATG
TCAAACTGAATTATGGTTTTTCAGTGCCTAATTCTGCTGTTAAAGGTGACACATTCAAAATAACTGTACC
TAAAGAATTAAACTTAAATGGTGTAACTTCAACTGCTAAAGTGCCACCAATTATGGCTGGAGATCAAGTA
TTGGCAAATGGTGTAATCGATAGTGATGGTAATGTTATTTATACATTTACAGACTATGTAAATACTAAAG
ATGATGTAAAAGCAACTTTGACCATGCCCGCTTATATTGACCCTGAAAATGTTAAAAAGACAGGTAATGT
GACATTGGCTACTGGCATAGGTAGTACAACAGCAAACAAAACAGTATTAGTAGATTATGAAAAATATGGT
AAGTTTTATAACTTATCTATTAAAGGTACAATTGACCAAATCGATAAAACAAATAATACGTATCGTCAGA
CAATTTATGTCAATCCAAGTGGAGATAACGTTATTGCGCCGGTTTTAACAGGTAATTTAAAACCAAATAC
GGATAGTAATGCATTAATAGATCAGCAAAATACAAGTATTAAAGTATATAAAGTAGATAATGCAGCTGAT
TTATCTGAAAGTTACTTTGTGAATCCAGAAAACTTTGAGGATGTCACTAATAGTGTGAATATTACATTCC
CAAATCCAAATCAATATAAAGTAGAGTTTAATACGCCTGATGATCAAATTACAACACCGTATATAGTAGT
TGTTAATGGTCATATTGATCCGAATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAAC
TCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTG
ACGGTATCGATAAACCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGA
TTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGT
GATTCTACATCAGATAGTGGTTCAGATTCAGCGAGTGATTCAGATTCAGCAAGTGATTCAGACTCAGCGA
GTGATTCAGATTCAGCAAGCGATTCCGACTCAGCGAGCGATTCCGACTCAGACAATGACTCGGATTCAGA
TAGCGATTCTGACTCAGACAGTGACTCAGATTCCGACAGTGACTCAGATTCAGATAGCGATTCTGACTCA
GACAGTGACTCAGATTCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCCGACAGTGATTCCGACT
CAGACAGCGATTCTGACTCCGACAGTGATTCCGACTCAGACAGCGATTCAGATTCCGACAGTGATTCCGA
CTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCA
GATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATT
CAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCAGACTCAGACAGTGATTCGGATTCAGCGAGTGA
TTCGGATTCAGATAGTGATTCCGACTCCGACAGTGACTCGGATTCAGATAGCGACTCAGACTCGGATAGC
GACTCGGATTCAGATAGCGATTCGGACTCAGATAGCGATTCAGAATCAGACAGCGATTCAGAATCAGACA
GCGATTCAGATTCAGACAGCGACTCAGACAGTGACTCAGATTCAGATAGTGACTCGGATTCAGCGAGTGA
TTCAGACTCAGGTAGTGACTCCGATTCATCAAGTGATTCCGACTCAGAAAGTGATTCAAATAGCGATTCC
GAGTCAGGTTCTAACAATAATGTAGTTCCGCCTAATTCACCTAAAAATGGTACTAATGCTTCTAATAAAA
ATGAGGCTAAAGATAGTAAAGAACCATTACCAGATACAGGTTCTGAAGATGAAGCAAATACGTCACTAAT
TTGGGGATTATTAGCATCAATAGGTTCATTACTACTTTTCAGAAGAAAAAAAGAAAATAAAGATAAGAAA
TA
-
Protein Sequence : Show Sequence
>YP_001331790.1 clumping factor A [Staphylococcus aureus subsp. aureus str. Newman]
MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSVTQSDSASNESKSNDSSSVSAAPKTDD
TNVSDTKTSSNTNNGETSVAQNPAQQETTQSSSTNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEE
LVNQTSNETTFNDTNTVSSVNSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSA
PRMRAFSLAAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFKITVP
KELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLTMPAYIDPENVKKTGNV
TLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNNTYRQTIYVNPSGDNVIAPVLTGNLKPNT
DSNALIDQQNTSIKVYKVDNAADLSESYFVNPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVV
VNGHIDPNSKGDLALRSTLYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPED
SDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSDNDSDSD
SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASD
SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASD
SDSGSDSDSSSDSDSESDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLI
WGLLASIGSLLLFRRKKENKDKK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Mice immunized with recombinant ClfA and challenged with S. aureus developed less-severe arthritis than did mice immunized with a control antigen (Josefsson et al., 2001).
full-length ClfA N123 is required for maximal protection both locally and systemically.(Lacey et al., 2017)
- Related Vaccine(s):
S. aureus ClfA Protein Vaccine
,
S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna
,
S. aureus DNA vaccine pClfaSrtD13
|
3. clfB |
-
Gene Name :
clfB
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
SIN33476
-
Other Database IDs :
CDD:309682
CDD:313621 CDD:307064
-
Taxonomy ID :
46170
-
Gene Strand (Orientation) :
?
-
Protein Name :
Fibrinogen-binding protein B
-
Protein pI :
3.66
-
Protein Weight :
93865.65
-
Protein Length :
1009
-
Protein Note :
predicted cleavage at residue 45
-
Protein Sequence : Show Sequence
>SIN33476.1 Fibrinogen-binding protein B [Staphylococcus aureus subsp. aureus]
MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQASEQSNDTTQSSKNNASADSEKNNMIE
TPQLNTTANDTSDISANTNSANVDSTTKPMSTQTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQ
EANSQVDNKTTNDANSIATNSELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADA
KGTNVNDKVTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNSNNTM
PIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKAPKSGTYDANINIADEM
FNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQTVFVNPKQRVLGNTWVYIKGYQDKIEES
SGKVSATDTKLRIFEVNDTSKLSDSYYADPNDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVE
GHYDNTGKNLKTQVIQENVDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDP
EPEPEPTPDPEPSPDPEPEPSPDPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSESDSDS
ESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDSDSDSDSDSDSDSDSDS
DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
DSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHKTDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQD
HKEKA
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Rauch et al., 2014)
|
4. cna |
-
Gene Name :
cna
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
VO ID :
VO_0012389
-
NCBI Nucleotide GI :
387879
-
NCBI Protein GI :
WP_001039420
-
Protein Accession :
AAA20874.1
-
3D structure: PDB ID :
2F68
-
Other Database IDs :
CDD:283410
CDD:283411 CDD:301706 CDD:212461
-
Taxonomy ID :
1280
-
Gene Strand (Orientation) :
?
-
Protein Name :
Cna B-type domain-containing protein
-
Protein pI :
6.09
-
Protein Weight :
124972.57
-
Protein Length :
1278
-
Protein Note :
Collagen binding domain; pfam05737
-
DNA Sequence : Show Sequence
>gi|387879|gb|M81736.1|STACNA Staphylococcus aureus collagen adhesin (cna) gene, complete cds
ATGCACTTGTATTCGTTATACTGTATATATTTTGCATAATAAAATAATAATATGAATTTTTGATAAATTT
CATTGAATAAGAACTAAATTAGTTTATAATTTATTATTAGTATCCTGTGGATATGACATAGAGTATAAGG
AGGGGTTTTTATGAACAAAAATGTGTTGAAGTTTATGGTCTTTATAATGTTATTAAATATCATCACACCT
TTATTTAATAAAAATGAAGCATTTGCAGCACGAGATATTTCATCAACGAATGTTACAGATTTAACTGTAT
CACCGTCTAAGATAGAAGATGGTGGTAAAACGACAGTAAAAATGACGTTCGACGATAAAAATGGAAAAAT
ACAAAATGGTGACATGATTAAAGTGGCATGGCCGACAAGCGGTACAGTAAAGATAGAGGGTTATAGTAAA
ACAGTACCATTAACTGTTAAAGGTGAACAGGTGGGTCAAGCAGTTATTACACCAGACGGTGCAACAATTA
CATTCAATGATAAAGTAGAAAAATTAAGTGATGTTTCGGGATTTGCAGAATTTGAAGTACAAGGAAGAAA
TTTAACGCAAACAAATACTTCAGATGACAAAGTAGCTACGATAACATCTGGGAATAAATCAACGAATGTT
ACGGTTCATAAAAGTGAAGCGGGAACAAGTAGTGTTTTCTATTATAAAACGGGAGATATGCTACCAGAAG
ATACGACACATGTACGATGGTTTTTAAATATTAACAATGAAAAAAGTTATGTATCGAAAGATATTACTAT
AAAGGATCAGATTCAAGGTGGACAGCAGTTAGATTTAAGCACATTAAACATTAATGTGACAGGTACACAT
AGCAATTATTATAGTGGACAAAGTGCAATTACTGATTTTGAAAAAGCCTTTCCAGGTTCTAAAATAACTG
TTGATAATACGAAGAACACAATTGATGTAACAATTCCACAAGGCTATGGGTCATATAATAGTTTTTCAAT
TAACTACAAAACCAAAATTACGAATGAACAGCAAAAAGAGTTTGTTAATAATTCACAAGCTTGGTATCAA
GAGCATGGTAAGGAAGAAGTGAACGGGAAATCATTTAATCATACTGTGCACAATATTAATGCTAATGCCG
GTATTGAAGGTACTGTAAAAGGTGAATTAAAAGTTTTAAAACAGGATAAAGATACCAAGGCTCCTATAGC
TAATGTAAAATTTAAACTTTCTAAAAAAGATGGATCAGTTGTAAAGGACAATCAAAAAGAAATTGAGATT
ATAACAGATGCAAACGGTATTGCTAATATTAAAGCGTTGCCTAGTGGAGACTATATTTTAAAAGAAATAG
AGGCGCCACGACCGTATACATTTGATAAGGATAAAGAATATCCGTTTACTATGAAAGATACAGATAATCA
GGGATATTTTACGACTATTGAAAATGCAAAAGCGATAGAAAAAACAAAAGATGTTTCTGCTCAAAAGGTT
TGGGAAGGCACTCAAAAAGTGAAACCAACGATTTATTTCAAGTTGTACAAACAAGATGACAATCAAAATA
CAACACCAGTAGACAAAGCAGAGATTAAAAAATTAGAAGATGGAACGACAAAAGTGACATGGTCTAATCT
TCCGGAAAATGACAAAAATGGCAAGGCTATTAAATATTTAGTTAAAGAAGTAAATGCTCAAGGTGAAGAT
ACAACACCAGAAGGATATACTAAAAAAGAAAATGGTTTAGTGGTTACTAATACTGAAAAACCAATCGAAA
CAACATCAATTAGTGGTGAAAAAGTATGGGACGACAAAGACAATCAAGATGGTAAGAGACCAGAAAAAGT
CAGTGTGAATTTATTGGCTAACGGGGAGAAAGTAAAAACGTTAGACGTGACATCTGAAACAAACTGGAAG
TACGAATTTAAAGACTTACCGAAGTATGATGAAGGAAAGAAAATAGAATATACAGTGACCGAAGATCACG
TAAAAGACTACACAACAGACATCAACGGTACGACAATAACGAACAAGTATACACCAGGAGAGACATCGGC
AACAGTAACAAAAAATTGGGATGACAATAATAACCAAGACGGAAAACGACCAACTGAAATCAAAGTTGAG
TTATATCAAGATGGAAAAGCAACAGGAAAAACGGCAATATTAAATGAATCTAATAACTGGACACATACGT
GGACAGGATTAGATGAAAAAGCAAAAGGACAACAAGTAAAATACACAGTCGAGGAATTAACAAAGGTCAA
AGGTTATACAACACATGTGGATAACAATGATATGGGTAACTTGATTGTGACGAATAAATATACGCCAGAA
ACAACATCAATTAGTGGTGAAAAAGTATGGGACGACAAAGACAATCAAGATGGTAAGAGACCAGAAAAAG
TCAGTGTGAATTTATTGGCTGATGGAGAGAAAGTAAAAACGTTAGACGTGACATCTGAAACAAACTGGAA
GTACGAATTTAAAGACTTACCGAAGTATGATGAAGGAAAGAAAATAGAATATACAGTGACCGAAGATCAC
GTAAAAGACTACACAACAGACATCAACGGTACGACAATAACGAACAAGTATACACCAGGAGAGACATCGG
CAACAGTAACAAAAAATTGGGATGACAATAATAACCAAGACGGAAAACGACCAACTGAAATCAAAGTTGA
GTTATATCAAGATGGAAAAGCAACAGGAAAAACGGCAATATTAAATGAATCTAATAACTGGACACATACG
TGGACAGGATTAGATGAAAAAGCAAAAGGACAACAAGTAAAATACACAGTCGAGGAATTAACAAAGGTCA
AAGGTTATACAACACATGTGGATAACAATGATATGGGCAACTTGATTGTGACGAATAAATATACGCCAGA
AACAACATCAATTAGTGGTGAAAAAGTATGGGACGACAAAGACAATCAAGATGGTAAGAGACCAGAAAAA
GTCAGTGTGAATTTATTGGCTAACGGAGAGAAAGTAAAAACGTTAGACGTGACATCTGAAACAAACTGGA
AGTACGAATTTAAAGACTTACCGAAGTATGATGAAGGAAAGAAAATAGAATATACAGTGACCGAAGATCA
CGTAAAAGACTACACAACAGACATCAACGGTACGACAATAACGAACAAGTATACACCAGGAGAGACATCG
GCAACAGTAACAAAAAATTGGGATGACAATAATAACCAAGACGGAAAACGACCAACTGAAATCAAAGTTG
AGTTATATCAAGATGGAAAAGCAACAGGAAAAACGGCAATATTAAATGAATCTAATAACTGGACACATAC
GTGGACAGGATTAGATGAAAAAGCAAAAGGACAACAAGTAAAATACACAGTCGATGAATTAACAAAAGTT
AATGGCTATACAACGCATGTGGATAACAATGATATGGGTAACTTGATTGTGACAAATAAATATACGCCGA
AAAAACCGAATAAACCAATCTATCCTGAAAAACCAAAAGACAAAACACCACCAACTAAACCTGATCATTC
TAATAAAGTTAAACCAACTCCCCCAGATAAGCCATCAAAAGTGGATAAGGATGATCAACCTAAAGATAAT
AAAACCAAACCTGAAAATCCTCTAAAAGAATTACCAAAAACTGGTATGAAGATTATAACTTCATGGATTA
CATGGGTATTTATAGGTATATTGGGACTGTATTTAATTTTAAGAAAAAGATTTAACTCATAAACCATTAT
AATTATTTTTATAGATAAGGCTATTCTTAGTTCTATGTATAATACATGTATATTAATAGGTCACTTTTAA
TCTGTATGTAAGCAGACTAAGAGTGGCCTTTTAAACAAATAAAAAAA
-
Protein Sequence : Show Sequence
>WP_001039420.1 Cna B-type domain-containing protein [Staphylococcus aureus]
MNKNVLKFMVFIMLLNIITPLFNKNEALAARDISSTNVTDLTVSPTKIEDGGKTTVKMTFDDKSVKIQNG
DTIKVAWPTSGTVKIEGYSKTVPLTVKGEQVGQAVITPDGATITFNDKVEKLSDVSGFAEFEVQGRNLTQ
TNTSDDKVATITSGNKSTNVTVHKSEAGTSSVFYYKTGDMLPEDTTHVRWFLNINNEKRYVSKDITIKDQ
IQGGQQLDLSTLNINVTGTHSNYYSGSNAITDFEKAFPGSKITVDNTKNTIDVTIPQGYGSYNSFSINYK
TKITNEQQKEFVNNSQAWYQEHGKEEVNGKSFNHTVHNINANAGIEGTVKGELKVLKQDKDTKAPIANVK
FKLSKKDGSVVKDNQKEIEIITDANGTANIKALPSGDYILKEIEAPAPYTFDKDKEYPFTMKDTDNQGYF
TTIENAKEIEKTKDVSAQKVWEGTQKVKPTIYFKLYKQDDNQNTTPVDKAEIKKLEDGTTKVTWSNLPEN
DKNGKTIKYLVKEVNAQGEDTTPEGYTKKENGLVVTNTEKPIETTSISGEKVWDDKDNQDGKRPEKVSVN
LLANGEKVKTLDVTSETNWKYEFKNLPKYDEGKKIEYTVTEDHVKDYTTDINGTTITNKYTPGETSATVT
KNWDDNNNQDGKRPTEIKVELYQDGKATGKTATLNESNNWTHTWAGLDEKAKGQQVKYTVEELTKVKGYT
THVDNNDMGNLIVTNKYTPETTSISGEKVWDDKDNQDGKRPEKVSVNLLANGEKVKAVDVTSETNWKYEF
KDLPKYDEGKKIEYTVTEDHVKDYTTDINGTTITNKYTPGETSATVTKNWDDNNNQDGKRPTEIKVELYQ
DGKATGKTAKLNESNNWTHTWTGLDEKAKGQQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPETTS
INGEKVWDDKDNQDGKRPEKVSINLLANGEKVKTLDVTSETNWKYEFKDLPKYDEGKKIEYTVTEDHVKD
YTTDINGTTITNKYTPGETSATVTKNWDDNNNQDGKRPTEIKVELYQDGKATGKTATLNESNNWTHTWAG
LDEKAKGQQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPEKPNKPIYPEKPKDKTPPTKPDHSNKV
KPTPPDKPSKVDKDDQPKDNKTKPENPLKELPKTGMKIITSWITWVFIGILGLYLILRKRFNS
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Mice were immunized with a recombinant fragment of CNA protein, and were then challenged with the S. aureus clinical isolate strain Phillips. At 14 d after inoculation, mortality in the collagen adhesin-vaccinated group was only 13%, compared with 87% in the control antigen immunized group (Nilsson et al., 1998).
Mice vaccinated with a combination of two Staphylococcus aureus antigens consisting of a recombinant collagen-binding protein (CnBP) and alpha-toxoid (alpha-toxoid) were significantly protected from intramammary challenge infection with S. aureus.(Mamo et al., 2000)
- Related Vaccine(s):
S. aureus CNA Protein Vaccine
,
S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna
|
5. Csa1A |
-
Gene Name :
Csa1A
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
4BIH_B
-
Other Database IDs :
CDD:282376
-
Taxonomy ID :
93061
-
Protein Name :
Csa1A
-
Protein pI :
6.96
-
Protein Weight :
28371.75
-
Protein Length :
318
-
Protein Note :
Protein of unknown function, DUF576; pfam04507
-
Protein Sequence : Show Sequence
>pdb|4BIH|B Chain B, Uncharacterized Lipoprotein Saouhsc_00053
MGSSHHHHHHENLYFQGGCGIGKEAEVKKSFEKTLSMYPIKNLEDLYDKEGYRDDQFDKNDKGTWIINSE
MVIQPNNEDMVAKGMVLYMNRNTKTTNGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKI
KKEIENFKFFVQYGDFKNLKNYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTSKAPKLLLKG
SGNLKGSSVGYKDIEFTFVEKKEENIYFSDSLDYKKSGDV
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Bagnoli et al., 2015)
|
6. efb |
-
Gene Name :
efb
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus subsp. aureus VRS11b
-
NCBI Protein GI :
387748005
-
Other Database IDs :
CDD:192953
-
Taxonomy ID :
1116225
-
Gene Strand (Orientation) :
?
-
Protein Name :
extracellular fibrinogen-binding protein Efb
-
Protein pI :
10.45
-
Protein Weight :
18630.02
-
Protein Length :
271
-
Protein Note :
Extracellular fibrinogen binding protein C terminal; pfam12199
-
Protein Sequence : Show Sequence
>EIK34704.1 extracellular fibrinogen-binding protein Efb [Staphylococcus aureus subsp. aureus VRS11b]
MKNKLIAKSLLTIAAIGITTTTIASTADASEGYGPREKKPVSINHNIVEYNDGTFKYQSRPKFNSTPKYI
KFKHDYNILEFNDGTFEYGARPQFNKPAAKTDATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLV
SFEYKVKKMVLQERIDNVLKQGLVR
-
Molecule Role :
Protective antigen
- Related Vaccine(s):
S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna
|
7. entA_1 |
-
Gene Name :
entA_1
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus subsp. aureus SK1585
-
NCBI Protein GI :
KFD31781
-
Taxonomy ID :
1274985
-
Protein Name :
sea
-
Protein pI :
6.05
-
Protein Weight :
28560.84
-
Protein Length :
316
-
Protein Note :
SEA
-
Protein Sequence : Show Sequence
>KFD31781.1 sea [Staphylococcus aureus subsp. aureus SK1585]
MGELEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKLDSKRLYNAKVSFANPTDLENKKTNDRLLKH
DLLFHDMFVNDDWKKDFKVEFENEALSKKFINKDIDIFAGNYGYGCHGGETNKTQCSYGGVTLSDNNKYD
NDKNIPCNLWIDGHQTEIELTAVKTKKKIVTIQELDVQLRNYLNEKYKLYEQGGDIVKGYVKYHNDDEKN
VEYDFYNLNGEYGHEVLKMYADNKTINSDKLHLDIYLFKS
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Nilsson et al., 1999)
|
8. esxA |
-
Gene Name :
esxA
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
A0A0H2XI99
-
Other Database IDs :
CDD:227179
-
Taxonomy ID :
367830
-
Protein Name :
ESAT-6 secretion system extracellular protein A
-
Protein pI :
4.34
-
Protein Weight :
13241.094
-
Protein Length :
225
-
Protein Note :
Ess extracellular protein A
-
Protein Sequence : Show Sequence
>sp|A0A0H2XI99.1|ESXA_STAA3 RecName: Full=ESAT-6 secretion system extracellular protein A; Short=Ess extracellular protein A
MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWEGQAFSRFEEQFQQLSPKVEKFAQLLE
EIKQQLNSTADAVQEQDQQLSNNFGLQ
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Bagnoli et al., 2015)
|
9. esxB |
-
Gene Name :
esxB
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
A0A0H2XIE9
-
Other Database IDs :
CDD:310537
-
Taxonomy ID :
367830
-
Protein Name :
ESAT-6 secretion system extracellular protein B
-
Protein pI :
4.94
-
Protein Weight :
13333.384
-
Protein Length :
232
-
Protein Note :
Ess extracellular protein B
-
Protein Sequence : Show Sequence
>sp|A0A0H2XIE9.1|ESXB_STAA3 RecName: Full=ESAT-6 secretion system extracellular protein B; Short=Ess extracellular protein B
MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEGSDWEGQFANKVKDVLLIMAKFQEELV
QPMADHQKAIDNLSQNLAKYDTLSIKQGLDRVNP
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Bagnoli et al., 2015)
|
10. Fhud2 |
-
Gene Name :
Fhud2
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
4B8Y_A
-
Other Database IDs :
CDD:223687
CDD:294188 CDD:238347
-
Taxonomy ID :
93061
-
Gene Strand (Orientation) :
?
-
Protein Name :
ferrichrome ABC transporter substrate-binding protein
-
Protein pI :
9.55
-
Protein Weight :
29595.83
-
Protein Length :
309
-
Protein Note :
NCBI Domains
-
Protein Sequence : Show Sequence
>pdb|4B8Y|A Chain A, Fhud2
MKAETKSYKMDDGKTVDIPKDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGD
VEKVAKEKPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWEETTAK
DGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQKLTAKAGWAEVKQEEIEK
YAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAGTYWYNDPYTLDFMRKDLKEKLIKAAK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Bagnoli et al., 2015)
|
11. fnbA |
-
Gene Name :
fnbA
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus subsp. aureus NCTC 8325
-
VO ID :
VO_0012392
-
NCBI Protein GI :
120457
-
Other Database IDs :
CDD:309682
CDD:331378 CDD:313621 CDD:308566 CDD:307064
-
Taxonomy ID :
93061
-
Gene Strand (Orientation) :
?
-
Protein Name :
Fibronectin-binding protein A
-
Protein pI :
4.36
-
Protein Weight :
105986.3
-
Protein Length :
1133
-
Protein Note :
FnbpA
-
Protein Sequence : Show Sequence
>sp|P14738.1|FNBA_STAA8 RecName: Full=Fibronectin-binding protein A; Short=FnbpA; Flags: Precursor
MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTTVEENGNSATDNKTSETQTTATNVNHI
EETQSYNATVTEQPSNATQVTTEEAPKAVQAPQTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQR
QVDLTPKKATQNQVAETQVEVAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEG
HNNTNKVEPHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGEVLEG
GKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELDVKYKDGIGNYYANLNG
SIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNGNQPKVRIFEYLGNNEDIAKSVYANTTDT
SKFKEVTSNMSGNLNLQNNGSYSLNIENLDKTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGY
TLTWDNGLVLYSNKANGNEKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAI
DGGGGYVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHADVVEYE
EDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIELVDELPEEHGQAQGPVEE
ITKNNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNI
VDIDFDSVPQIHGQNKGNQSFEEDTEKDKPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSY
QFGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPP
TPEVPSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKPQSKKS
ELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Mice were immunized with fusion proteins encompassing the fibronectin-binding domain of a staphylococcal fibronectin-binding protein (FnBP-A). Vaccination of mice with recombinant FnBP resulted in significant protection against challenge with S. aureus (Mamo et al., 1994).
- Related Vaccine(s):
S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna
,
S. aureus FnbA Protein Vaccine
|
12. FnbpA |
-
Gene Name :
FnbpA
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
EEV76353
-
Other Database IDs :
CDD:287406
-
Taxonomy ID :
553573
-
Gene Strand (Orientation) :
?
-
Protein Name :
fibrinogen-binding protein A
-
Protein pI :
3.38
-
Protein Weight :
97931.47
-
Protein Length :
1076
-
Protein Note :
C-terminus of bacterial fibrinogen-binding adhesin; pfam10425
-
Protein Sequence : Show Sequence
>EEV76353.1 fibrinogen-binding protein A [Staphylococcus aureus A8115]
MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSVTQSDSASNESKSNDSSSVSAAPKTDD
TNVSDTKTSSNTNNGETSVAQNPAQQETTQSSSTNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEE
LVNQTSNETTSNDTNTVSSVNSPQNSTNAENVSTTQDTSTEATPSNNESAPQNTDASNKDVVSQAVNPST
PRMRAFSLAAVAADAPAAGTDITNQLTDVKVTIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFKITVP
KELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDNKENVTANITMPAYIDPENVTKTGNV
TLTTGIGTNTASKTVLIDYEKYGQFHNLSIKGTIDQIDKTNNTYRQTIYVNPSGDNVVLPALTGNLIPNT
KSNALIDAKNTDIKVYRVDNANDLSESYYVNPSDFEDVTNQVRISFPNANQYKVEFPTDDDQITTPYIVV
VNGHIDPASTGDLALRSTFYGYDSNFIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPED
SDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSA
SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSD
SDSDSDSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDND
SDSDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASLGSLLLFR
RKKENKDKK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Veloso et al., 2015)
|
13. gapC |
-
Gene Name :
gapC
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
AAR13672
-
Other Database IDs :
CDD:278473
CDD:273675 CDD:280894
-
Taxonomy ID :
1280
-
Protein Name :
GapC
-
Protein pI :
4.71
-
Protein Weight :
33224.4
-
Protein Length :
383
-
Protein Note :
Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain; pfam00044
-
Protein Sequence : Show Sequence
>AAR13672.1 GapC [Staphylococcus aureus]
MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHLLKYDTMQGRFTGEVEVVDGGFRVNGK
EVKSFSEPDASKLPWKDLNIDVVLECTGFYTDKDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELD
GSETVVSGASCTTNSLAPVAKVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNS
TGAAKAIGKVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKPDVTVEQVNEAMKNASNESFGYTEDEIVS
SDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAELSK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Yu et al., 2014)
|
14. gst |
-
Gene Name :
gst
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
EES94142
-
Taxonomy ID :
450394
-
Protein Name :
glutathione S-transferase
-
Protein pI :
6.24
-
Protein Weight :
9384
-
Protein Length :
182
-
Protein Note :
COG: COG0625; Pfam: PF02798; InterPro: IPR012335
-
Protein Sequence : Show Sequence
>EES94142.1 glutathione S-transferase, partial [Staphylococcus aureus subsp. aureus USA300_TCH959]
TLNHRQKELPMKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYFAVNPKGQVPALLLDDG
TLLTEGVAIM
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Cui et al., 2005)
|
15. hla |
-
Gene Name :
hla
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
AMQ79365
-
Other Database IDs :
CDD:273388
-
Taxonomy ID :
1280
-
Protein Name :
hla
-
Protein pI :
9.19
-
Protein Weight :
34393.79
-
Protein Length :
365
-
Protein Note :
beta-channel forming cytolysin; TIGR01002
-
Protein Sequence : Show Sequence
>AMQ79365.1 hla [Staphylococcus aureus]
MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNTTVKTGDLVTYDKENGMHKKVFYSFID
DKNHNKKILVIRTKGTIAGQYRVYSEEGANKSGLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMS
TLTYGFNGNVTGDDSGKIGGLIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDR
DSWNPVYGNQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKATKQQTNIDVIYERVRD
DYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Bagnoli et al., 2015)
|
16. hlgB |
-
Gene Name :
hlgB
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
AKB00489
-
Other Database IDs :
CDD:273388
-
Taxonomy ID :
1280
-
Protein Name :
gamma-hemolysin component C precursor, HlgB
-
Protein pI :
9.81
-
Protein Weight :
35450.76
-
Protein Length :
411
-
Protein Note :
beta-channel forming cytolysin; TIGR01002
-
Protein Sequence : Show Sequence
>AKB00489.1 gamma-hemolysin component C precursor, HlgB [Staphylococcus aureus]
MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTLYKTTATADSDKFKISQILTFNFIKDK
SYDKDTLVLKATGNINSGFVKPNPNDYDFSKLYWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTL
GYTFGGDISISNGLSGGLNGNTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSF
HPTYGNELFLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMDLYQI
RWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Delfani et al., 2016)
|
17. isdB |
-
Gene Name :
isdB
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus subsp. aureus str. Newman
-
NCBI Gene ID :
5330644
-
NCBI Protein GI :
151221252
-
Locus Tag :
NWMN_1040
-
Genbank Accession :
AP009351
-
Protein Accession :
YP_001332074
-
Other Database IDs :
CDD:213844
-
Taxonomy ID :
426430
-
Gene Starting Position :
1142079
-
Gene Ending Position :
1144016
-
Gene Strand (Orientation) :
-
-
Protein Name :
iron-regulated heme-iron binding protein IsdB
-
Protein pI :
9.6
-
Protein Weight :
67826.06
-
Protein Length :
645
-
Protein Note :
heme uptake protein IsdB; TIGR03657
-
DNA Sequence : Show Sequence
>NC_009641.1:1142079-1144016 Staphylococcus aureus subsp. aureus str. Newman DNA, complete genome
ATTAGTTTTTACGTTTTCTAGGTAATACGAATGCAACGATGCTACTTAAAGCTAATAATGCCATTAATGG
TAATGTCATATCTTTATTTGATTCTTCACCAGTTTGTGGTAATGATTTTGCTTTATTTTCTTGTGTATTT
TTATTGTTTTGGCTTTGAGTGTGTCCATCATTTGTGTTTTTAATGTTTGCTTTTTGTAATGGAGCACTAT
CTTTTGCTTCGCTAGAACCTGCTGAAGTTTGAACAACATCTTTTGTTGTTTTTGATGAAGCAGTTGTTGG
TTTTGCAACATTTTGAGTCGTAGATACTACCTTAGTTGGAGTTGTACTACTTGATTCTACTTCACCTTTA
GTTGGTTTTGTAGCAGGCGTTTTGTCTTTACCTGACTCACTAGATGCGTCATTTTCTTTTTCAACACTTG
GTAATTGTTTATTGTCATCTTTTTGGCTGTCTTGTTTTTGTGATTCTTTTTCAACAGGTGATGGTGTTGG
TTTGCTAGGCGTAGCTGGAGTAGCTTCCTTCTTAGCTGAGTTATCTTGTTGTTCTTTTTTGTTAGATTTA
TCGGTATTGGCTTTTGTAAATGCTTCTTTATCAACGATTCTGACATGGTATTGTCCATCATAATCAATCG
TTTTTACGTGAACTTTAACGATAGCATCATATAGAGTTTTACCTTCAACATATGGGAAAATAATTGTTCT
AGTATTATTTTTAGCATCTTTGCTTATAGTTCTAACACGTTGACCTTCAACCATGAAATCTTTCCAGTAA
TCGTCATTAGTAGTTTCCATGACCATATATTTTTTGCCGTTAAGCATACCTGTTTTAATAGGGTGTTTAA
CAAAAGTATCCATCATAGATTCGTTATTCTCAACACTTTCATAAACAACATATTTTGTATCTTGTAAATC
AGTCATTTTTTCATTTGTTGGTTGTACATTTTGGAATTCAGTAATAGCTGATTTCACTTGCTCATCTAAA
GCTTTCTTTGTATCCTCTAATTTCTTCTTGTACTCAGCCTTTAATTTTTCAGGAAGTTTATCTTGAATTT
TATTTAATTCATAAACTTGTCTTTCTAGTGTTTTCGCTTTTTTATATGGCGCTAATAATTTTTCAGCTTT
ATAATCTTCTTCAGTTTTGAATTTATCTGCACTGTTATAAATTGGTTGTGCGAATTCCATTAATGTGTAA
TCGTATTTTTCTTCTTTGTTATTGAAGTGTGTTGAACTAACAATTTTAACAGCTTTTGTTCCGTTTGATA
CAGAGAAGCGAATGTAAGCATAATCTTTAACAGTATCGTATGATACTAATTTAATTGGCAACTTTTTGTC
ACCTTCATAAACTTCAAATTTTCTCCAAAATTGACCTGATTGTAATCCTAATTCAATTTCTGGTTTTGAA
TCAGTGAAAATAACTCTAGCAGGTTTAACAGAACTTGCATAATGATAAAACTGTTGAGTTCCATCTTTCT
TTTTCATTTCAAAATCAATTGGACGAGAGTTTGGTGCGCTATGATCTTTGTCTTTTATTGCAGGGTTTTT
AATCGCTTCTCTAAGTTCCTGATTCAAAATAGGATATGTATTGTTAGTGGCTTTTGCTGCTGGTTTAACT
TCTTTTGTTTCCTTAGGGGCTTTAACTTCTTTAACTTCTTTAGCTTCTTTTGTTTCAGAAGTAGGGGCCT
CAACTTCTTTATTAGATACTGAGACAGCATTAGCTACTGGTTTAGTTTCTGGAGCTTTTTCAGATGTTGT
TGTTGGACTTGCAACTGCTTCAGTTTTTGGTTGTGCTTCTGTATTTGTACCACCTGTTTCTTCAGCTGCT
GCTTGTGCTTCGCCATTTGACATTAATAATAAAAGTGTACTAATTGCTACAGATGCAACGCCTAGTGATG
ACTTTCTAATTGAATAAAATGATTTAAATTCTTTTTGCTGTTTGTTCA
-
Protein Sequence : Show Sequence
>YP_001332074.1 iron-regulated heme-iron binding protein IsdB [Staphylococcus aureus subsp. aureus str. Newman]
MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEETGGTNTEAQPKTEAVASPTTTSEKAP
ETKPVANAVSVSNKEVEAPTSETKEAKEVKEVKAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKD
KDHSAPNSRPIDFEMKKKDGTQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIK
LVSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEKLLA
PYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQNVQPTNEKMTDLQDTKY
VVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWKDFMVEGQRVRTISKDAKNNTRTIIFPY
VEGKTLYDAIVKVHVKTIDYDGQYHVRIVDKEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVE
KESQKQDSQKDDNKQLPSVEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSK
TTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLPLMALLA
LSSIVAFVLPRKRKN
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
In preclinical studies, a isdB vaccine has shown protection from lethal infection in mice (Harro et al., 2010).
Iron-regulated surface determinant B (IsdB) of Staphylococcus aureus (S. aureus) is a highly conserved surface protein that can induce protective CD4(+) T-cell immune response. A pivotal role of CD4(+) T-cells in effective immunity against S. aureus infection has been proved(Yu et al., 2015)
|
18. IsdH |
-
Gene Name :
IsdH
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus subsp. aureus 71193
-
NCBI Protein GI :
AFH69961
-
Other Database IDs :
CDD:331945
-
Taxonomy ID :
1155084
-
Protein Name :
IsdH
-
Protein pI :
5.06
-
Protein Weight :
94952.35
-
Protein Length :
970
-
Protein Note :
methicillin-sensitive; sequence type ST398
-
Protein Sequence : Show Sequence
>AFH69961.1 IsdH [Staphylococcus aureus subsp. aureus 71193]
MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENTNTSDKISENQNNNATTTQPPKDTNQT
QPATQPTNTAKTYPAADESLKDAIKNPAVENKEHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYT
KKKAEVELDINTASTWKKFEVYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGAET
NYDYTKLVFAKPIYNDPSLVKSDTNDAVASNDQSSSDASNQTNTNTSNQNTSTTNNASDQPQATTNMSQP
AKPVSSANADQASSQPAHETNSNGNTNDKTNASSNQSNGNQQYPPADESLQDAIKNPAIIDKEHTADNWR
PIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKTASTWKKFEVYEGDKKLPVELVSYDSDKD
YAYIRFPVSNGAREVKIVSSIEYGENFHEDYDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTL
ERQVYELEKLQDKLPEKYKAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEEN
SESVMDGFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYVSDKAIYN
AIVKVVVANIGYEGQYHVRIVNQDIKTKDDDTSQNNTSEPLNVQTGQENKVSATDTAENSSTATNPKDAS
DKADVIEPESDVVKVTDSNIDKDAHHDVDHLSDMSDNTHLDKYDLKEMDTQIAKDTDKGVDKDADNSVGM
SSNVDTEKDINKNEGKVIQLAHNTDKNNHTGKAAKLDGVRQNYNNIDKVTDKKTTEHPSNDIHKTVDKTV
KTKEKSGTPSKENKLSQSKMLPKTGETTSSQTWWGLYALLGMLALFIPKFRKEPK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Clarke et al., 2006)
|
19. lukF |
-
Gene Name :
lukF
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
CRL36945
-
Other Database IDs :
CDD:273388
-
Taxonomy ID :
1280
-
Protein Name :
leukotoxin LukF
-
Protein pI :
9.51
-
Protein Weight :
36115.25
-
Protein Length :
385
-
Protein Note :
beta-channel forming cytolysin; TIGR01002
-
Protein Sequence : Show Sequence
>CRL36945.1 leukotoxin LukF [Staphylococcus aureus]
MKMKKLVKSSIASSIALILLSNTVDAAQHITPVSEKKVDDKITLYKTTATSDNDKLNLSQILIFNFIKDK
SYDKDTLILKAAGNINSGYKKPNPKDYNYSHFYWGGKYNVSISSESNENVNVVDYAPKNQNEEFQVQQTL
GYSYGGDINISNGLSGGGNGSKSFSETINYKQESYRTTIDRKTNHKFIGWGVEAHKIMNNGWGPYGRDSH
EDTYGNELFLGSRQSSSNAGQNFMPTHQMPLLSRGNFNPEFISVLSHKQNDTKKSKIKVTYQREMDRYTN
QWNGLHWIGNNYKNQNTVTFTSTYEVDWQNHSVKLLGTDSKETNPGV
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Karauzum et al., 2013)
|
20. lukS |
-
Gene Name :
lukS
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
CAA51251
-
Other Database IDs :
CDD:285242
GOA:Q53746 HSSP: 1PVL InterPro: IPR001340 InterPro: IPR003963 InterPro: IPR016183 UniProtKB/TrEMBL: Q53746
-
Taxonomy ID :
1280
-
Protein Name :
leucocidin S
-
Protein pI :
9.34
-
Protein Weight :
33903.95
-
Protein Length :
359
-
Protein Note :
Leukocidin/Hemolysin toxin family; pfam07968
-
Protein Sequence : Show Sequence
>CAA51251.1 lukS [Staphylococcus aureus]
MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDCAEVVKRTEDTSCDKWGVTQNIQFDFVKDKKYN
KDALILKMQGFINSKTTYYNYKNTDHIKAMRWPFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYN
IGGNFNSGPSTGGNGSFNYSKTISYNQQNYISEVEHQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYK
PYSQNPRDYFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGNSYLE
GSRIHKAFVNRNYTVKYEVNWKTHEIKVKGHN
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Karauzum et al., 2013)
|
21. MecA |
-
Gene Name :
MecA
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus subsp. aureus MRSA252
-
VO ID :
VO_0012391
-
NCBI Gene ID :
2861157
-
NCBI Protein GI :
49482291
-
Locus Tag :
SAR0039
-
Genbank Accession :
BX571856
-
Protein Accession :
YP_039515
-
3D structure: PDB ID :
1VQQ
-
Taxonomy ID :
282458
-
Gene Starting Position :
44918
-
Gene Ending Position :
46924
-
Gene Strand (Orientation) :
-
-
Protein Name :
penicillin-binding protein 2 prime
-
Protein pI :
9.19
-
Protein Weight :
72330.63
-
Protein Length :
668
-
Protein Note :
Identical to Staphylococcus epidermidis, and Staphylococcus aureus penicillin-binding protein 2 prime MecA TR:Q54113 (EMBL: X52592) (668 aa) fasta scores: E(): 0, 100.000% id in 668 aa, and to Staphylococcus sciuri methicillin resistanc protein MecA2 TR:O54283 (EMBL: Y13095) (668 aa) fasta scores: E(): 0, 99.102% id in 668 aa
-
DNA Sequence : Show Sequence
>NC_002952.2:44918-46924 Staphylococcus aureus subsp. aureus strain MRSA252, complete genome
GTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTT
GGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTA
TCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAG
TACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTG
TTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTC
GTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCATTATTTTCTAATGCGCTATAGATTGAAAGGATCT
GTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATT
TTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTC
ATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATT
CTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCATATCTTGTAACGTTGTAACCACCCCAAGA
TTTATCTTTTTGCCAACCTTTACCATCGATTTTATAACTTGTTTTATCGTCTAATGTTTTGTTATTTAAC
CCAATCATTGCTGTTAATATTTTTTGAGTTGAACCTGGTGAAGTTGTAATCTGGAACTTGTTGAGCAGAG
GTTCTTTTTTATCTTCGGTTAATTTATTATATTCTTCGTTACTCATGCCATACATAAATGGATAGACGTC
ATATGAAGGTGTGCTTACAAGTGCTAATAATTCACCTGTTTGAGGGTGGATAGCAGTACCTGAGCCATAA
TCATTTTTCATGTTGTTATAAATACTCTTTTGAACTTTAGCATCAATAGTTAGTTGAATATCTTTGCCAT
CTTTTTTCTTTTTCTCTATTAATGTATGTGCGATTGTATTGCTATTATCGTCAACGATTGTGACACGATA
GCCATCTTCATGTTGGAGCTTTTTATCGTAAAGTTTTTCGAGTCCCTTTTTACCAATAACTGCATCATCT
TTATAGCCTTTATATTCTTTTTGTTTTAATTCTTCAGAGTTAATGGGACCAACATAACCTAATAGATGTG
AAGTCGCTTTTCCTAGAGGATAGTTACGACTTTCTGTTTCATTAGTTGTAAGATGAAATTTTTTTGCGAA
ATCACTTAAATATTCATCCATTTTTTTAACGGTTTTAAGTGGAACGAAGGTATCATCTTGTACCCAATTT
TGATCCATTTGTTGTTTGATATAGTCTTCAGAAATACTTAGTTCTTTAGCGATTGCTTTATAATCTTTTT
TAGATACATTCTTTGGAACGATGCCTATCTCATATGCTGTTCCTGTATTGGCCAATTCCACATTGTTTCG
GTCTAAAATTTTACCACGTTCTGATTTTAAATTTTCAATATGTATGCTTTGGTCTTTCTGCATTCCTGGA
ATAATGACGCTATGATCCCAATCTAACTTCCACATACCATCTTCTTTAACAAAATTAAATTGAACGTTGC
GATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTT
TTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCA
GTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTT
TATCTTCAATTGCATCAATAGTATTATTAATTTCTTTATCTTTTGAAGCATAAAAATATATACCAAACCC
GACAACTACAACTATTAAAATAAGTGGAACAATTTTTATCTTTTTCA
-
Protein Sequence : Show Sequence
>YP_039515.1 penicillin-binding protein 2 prime [Staphylococcus aureus subsp. aureus MRSA252]
MKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFKQVYKDSSYISKSDNGEVEMTERPIKIY
NSLGVKDINIQDRKIKKVSKNKKRVDAQYKIKTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQ
SIHIENLKSERGKILDRNNVELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDT
FVPLKTVKKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDDAVIGK
KGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNNMKNDYGSGTAI
HPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPLLNKFQITTSPGSTQKILTAMIGLNNKTLD
DKTSYKIDGKGWQKDKSWGGYNVTRYEVVNGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGE
DIPSDYPFYNAQISNKNLDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKK
NIISKENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKDNPNMMMA
INVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
An internal region from the transpeptidase domain from the PBP2a (encoded by mecA) was cloned into a mammalian expression vector, to be used as DNA vaccine in a Murine model. After three sets of DNA vaccination, the immune response represented by antibodies against a fragment of PBP2a was evaluated by enzyme linked immunosorbent assay (ELISA), showing a significant antibody response. The antibacterial effect of the DNA vaccine was evaluated by intraperitoneal immunization and challenge with a sublethal dose of MRSA for 7 days in mice. After the challenge, the number of bacteria from kidneys from immunized and non-immunized mice were determined. Kidneys from immunized mice had 1000 times less on bacteria than the positive controls (non-immunized mice) (Senna et al., 2003).
- Related Vaccine(s):
S. aureus DNA Vaccine encoding PBP2a Protein
|
22. mntC |
-
Gene Name :
mntC
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
AAL50778
-
Other Database IDs :
CDD:320865
CDD:238347
-
Taxonomy ID :
1280
-
Gene Strand (Orientation) :
?
-
Protein Name :
MntC
-
Protein pI :
9.02
-
Protein Weight :
33762.1
-
Protein Length :
359
-
Protein Note :
Helical backbone metal receptor (TroA-like domain). These proteins have been shown to function in the ABC transport of ferric siderophores and metal ions such as Mn2+, Fe3+, Cu2+ and/or Zn2+. Their ligand binding site is formed in the interface between...; cl00262
-
Protein Sequence : Show Sequence
>AAL50778.1 MntC [Staphylococcus aureus]
MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYDMAKNVGGDNVDIHSIVPVGQDPHEYE
VKPKDIKKLTDADVILYNGLNLETGNGWFEKALEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPH
AWLSLDNGIKYVKTIQQTFIDNDKKHKADYEKQGNKYIAQLEKLNNDSKDSKDKFNDIPKEQRAMITSEG
AFKYFSKQYGITPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFGE
VYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Yang et al., 2016)
|
23. sasA |
-
Gene Name :
sasA
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
AAR15189
-
Other Database IDs :
CDD:301310
-
Taxonomy ID :
1280
-
Protein Name :
surface protein SasA
-
Protein pI :
10.47
-
Protein Weight :
15925.95
-
Protein Length :
224
-
Protein Note :
legume lectins; cl14058
-
Protein Sequence : Show Sequence
>AAR15189.1 surface protein SasA, partial [Staphylococcus aureus]
TKSVTSTSSTEQPINTSTNQSTASNNTSQSTTPTSANLNKTSTTSTSTAPVKLRTFSRLAMSTFASAATT
TALTANTITVNKDNLKQYMTTSGNATYDQSTGVVTLTQDTTSQKGAITLGTRIDSNKSFHFSGKVNLGNK
YEGHGNGGDGIGFA
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Yang et al., 2017)
|
24. sdrD |
-
Gene Name :
sdrD
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
CAA06651
-
Other Database IDs :
CDD:309682
CDD:313621 CDD:316170 CDD:319228 CDD:307064 GOA:O86488 InterPro: IPR001899 InterPro: IPR003367 InterPro: IPR005877 InterPro: IPR008454 InterPro: IPR008966 UniProtKB/TrEMBL: O86488
-
Taxonomy ID :
426430
-
Protein Name :
SdrD
-
Protein pI :
4.04
-
Protein Weight :
134390.88
-
Protein Length :
1376
-
Protein Note :
YSIRK type signal peptide; pfam04650
-
Protein Sequence : Show Sequence
>CAA06651.1 sdrD [Staphylococcus aureus]
MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFGLGNQEAKAAESTNKELNEATTSASDN
QSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNETTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKST
NEDLNTKQTISNQEALQPDLQENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSN
NENNADIILPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLSLKSQ
ITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTANNLITYTFTDYVDRFN
SVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQYPDYVVNEKNSIGSAFTETVSHVGNKEN
PGYYKQTIYVNPSENSLTNAKLKVQAYHSSYPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDV
TNQYLQKITYGDNNSAVIDFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNN
QSGGAGQEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPNLPNGDY
RVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKYNLGDYVWEDTNKNGIQDQ
DEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGNYKVEFTTPEGYTPTTVTSGSDIEKDSNGLT
TTGVINGADNMTLDSGFYKTPKYNLGNYVWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDG
KYQFTGLENGTYKVEFETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVW
EDTNKNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGYTPTSVTSG
NDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVKVTLLNEKGE
VIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDADGGEVDVTITDHDDFTLDNGYYE
EETSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDS
DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHT
PVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKKQNK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Rauch et al., 2014)
|
25. seb |
-
Gene Name :
seb
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus subsp. aureus COL
-
NCBI Protein GI :
AAL04126
-
Other Database IDs :
CDD:279467
CDD:280949
-
Taxonomy ID :
93062
-
Protein Name :
Seb
-
Protein pI :
8.81
-
Protein Weight :
30196.07
-
Protein Length :
329
-
Protein Note :
pathogenicity island 3
-
Protein Sequence : Show Sequence
>AAL04126.1 Seb [Staphylococcus aureus subsp. aureus COL]
MYKRLFISHVILIFALILVISTPNVLAESQPDPKPDELHKSSKFTGLMENMKVLYDDNHVSAINVKSIDQ
FLYFDLIYSIKDTKLGNYDNVRVEFKNKDLADKYKDKYVDVFGANYYYQCYFSKKTNDINSHQTDKRKTC
MYGGVTEHNGNQLDKYRSITVRVFEDGKNLLSFDVQTNKKKVTAQELDYLTRHYLVKNKKLYEFNNSPYE
TGYIKFIENENSFWYDMMPAPGDKFDQSKYLMMYNDNKMVDSKDVKIEVYLTTKKK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Zhao et al., 2014)
|
26. spa |
-
Gene Name :
spa
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
WP_000728763
-
Other Database IDs :
CDD:309682
CDD:280394 CDD:331228 CDD:212030
-
Taxonomy ID :
2
-
Gene Strand (Orientation) :
?
-
Protein Name :
immunoglobulin G-binding protein A
-
Protein pI :
5.17
-
Protein Weight :
55778.05
-
Protein Length :
593
-
Protein Note :
YSIRK type signal peptide; pfam04650
-
Protein Sequence : Show Sequence
>WP_000728763.1 MULTISPECIES: immunoglobulin G-binding protein A [Bacteria]
MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQNAFYQVLNMPNLNADQRNGFIQSLKD
DPSQSANVLGEAQKLNDSQAPKADAQQNNFNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVL
GEAKKLNESQAPKADNNFNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAP
KADNKFNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQN
AFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKPGKEDNNKPGKEDNNKP
GKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDGNKPGKEDGNGVHVVKPG
DTVNDIAKANGTTADKIAADNKLADKNMIKPGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFG
GLSLALGAALLAGRRREL
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Pozzi et al., 2015)
|
27. staphylococcal enterotoxin A |
-
Gene Name :
staphylococcal enterotoxin A
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
AAA26681
-
Other Database IDs :
CDD:279467
CDD:280949
-
Taxonomy ID :
1280
-
Protein Name :
staphylococcal enterotoxin A
-
Protein pI :
8.15
-
Protein Weight :
28651.08
-
Protein Length :
337
-
Protein Note :
staphylococcal enterotoxin A signal peptide
-
Protein Sequence : Show Sequence
>AAA26681.1 staphylococcal enterotoxin A precursor [Staphylococcus aureus]
MKKTAFTLLLFIALTLTTSPLVNGSEKSEEINEKDLRKKSELQGTALGNLKQIYYYNEKAKTENKESHDQ
FLQHTILFKGFFTDHSWYNDLLVDFDSKDIVDKYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDN
NRLTEEKKVPINLWLDGKQNTVPLETVKTNKKNVTVQELDLQARRYLQEKYNLYNSDVFDGKVQRGLIVF
HTSTEPSVNYDLFGAQGQYSNTLLRIYRDNKTINSENMHIDIYLYTS
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Reddy et al., 2015)
|
28. traP |
-
Gene Name :
traP
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
ADK12000
-
Other Database IDs :
CDD:292771
-
Taxonomy ID :
1280
-
Protein Name :
target of RNAIII-activating protein
-
Protein pI :
7.23
-
Protein Weight :
20101.19
-
Protein Length :
243
-
Protein Note :
TraP
-
Protein Sequence : Show Sequence
>ADK12000.1 target of RNAIII-activating protein [Staphylococcus aureus]
MKKLYTSYGTYGFLNQIKINNPSHHLFQFSTADSSVIFEETEEKTVLKSPSIYEVIKEIGAFNEDHFYCA
IFIPSTEDHVYQLEKKLISVDDNFKNFGGFKSYRLLRPVKGTTYKIYFGFADRQTYEDFKNSDAFKDHFS
KEALSHYFGSSGQHSSYFERYLYPIKE
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Yu et al., 2014)
|
29. TSST-1 |
-
Gene Name :
TSST-1
-
Sequence Strain (Species/Organism) :
Staphylococcus aureus
-
NCBI Protein GI :
AAA26682
-
Other Database IDs :
CDD:332684
-
Taxonomy ID :
1280
-
Protein Name :
toxic shock syndrome toxin-1
-
Protein pI :
9.34
-
Protein Weight :
25910.55
-
Protein Length :
314
-
Protein Note :
toxic shock syndrome toxin-1 signal peptide
-
Protein Sequence : Show Sequence
>AAA26682.1 toxic shock syndrome toxin-1 precursor [Staphylococcus aureus]
MNKKLLMNFFIVSPLLLATTATDFTPVPLSSNQIIKTAKASTNDNIKDLLDWYSSGSDTFTNSEVLDNSL
GSMRIKNTDGSISLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIEL
PLKVKVHGKDSPLKYGPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSK
KFEYNTEKPPINIDEIKTIEAEIN
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Narita et al., 2015)
|
III. Vaccine Related Host Genes |
1. Ifng (Interferon gamma) |
-
Gene Name :
Ifng (Interferon gamma)
-
Sequence Strain (Species/Organism) :
Mouse
-
NCBI Gene ID :
15978
-
NCBI Protein GI :
33468859
-
Genbank Accession :
NM_008337
-
Protein Accession :
NP_032363.1
-
Other Database IDs :
MGI:107656; UniProt: P01580
-
Taxonomy ID :
10090
-
Gene Strand (Orientation) :
?
-
DNA Sequence : Show Sequence
>gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
AGTATACCTTTGCAACC
-
Protein Sequence : Show Sequence
>gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
LLPESSLRKRKRSRC
-
Molecule Role Annotation :
IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
-
Additional Molecule Role :
Vaximmutor
-
Additional Molecule Role Annotation :
The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
- Related Vaccine(s):
Staphylococcus aureus aroA mutant vaccine
|
2. Il4 (interleukin 4) |
-
Gene Name :
Il4 (interleukin 4)
-
Sequence Strain (Species/Organism) :
Mus musculus
-
NCBI Gene ID :
16189
-
NCBI Protein GI :
10946584
-
Locus Tag :
RP23-188H3.4
-
Genbank Accession :
NM_021283.1
-
Protein Accession :
NM_021283.1
-
Taxonomy ID :
10090
-
Gene Strand (Orientation) :
?
-
Protein Name :
IL-4, Interleukin 4
-
DNA Sequence : Show Sequence
>gi|10946583|ref|NM_021283.1| Mus musculus interleukin 4 (Il4), mRNA
GGATCCCCGGGCAGAGCTGGGGGGGGATTTGTTAGCATCTCTTGATAAACTTAATTGTCTCTCGTCACTG
ACGGCACAGAGCTATTGATGGGTCTCAACCCCCAGCTAGTTGTCATCCTGCTCTTCTTTCTCGAATGTAC
CAGGAGCCATATCCACGGATGCGACAAAAATCACTTGAGAGAGATCATCGGCATTTTGAACGAGGTCACA
GGAGAAGGGACGCCATGCACGGAGATGGATGTGCCAAACGTCCTCACAGCAACGAAGAACACCACAGAGA
GTGAGCTCGTCTGTAGGGCTTCCAAGGTGCTTCGCATATTTTATTTAAAACATGGGAAAACTCCATGCTT
GAAGAAGAACTCTAGTGTTCTCATGGAGCTGCAGAGACTCTTTCGGGCTTTTCGATGCCTGGATTCATCG
ATAAGCTGCACCATGAATGAGTCCAAGTCCACATCACTGAAAGACTTCCTGGAAAGCCTAAAGAGCATCA
TGCAAATGGATTACTCGTAGTACTGAGCCACCATGCTTTAACTTATGAATTTTTAATGGTTTTATTTTAA
TATTTATATATTTATAATTCATAAAATAAAATATTTGTATAATGT
-
Protein Sequence : Show Sequence
>gi|10946584|ref|NP_067258.1| interleukin 4 [Mus musculus]
MGLNPQLVVILLFFLECTRSHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCR
ASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS
-
Molecule Role :
Vaximmutor
-
Molecule Role Annotation :
IL-4 plays an important role in Th2 immune response.
- Related Vaccine(s):
Staphylococcus aureus aroA mutant vaccine
|
IV. Vaccine Information |
|
|
|
|
|
|
1. S. aureus ClfA Protein Vaccine |
a. Vaccine Ontology ID: |
VO_0004047 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Antigen |
Recombinant ClfA protein (Josefsson et al., 2001). |
e. Gene Engineering of
ClfA |
- Type:
Recombinant protein preparation
- Description:
Expression and purification of recombinant proteins.Vector pCF40 expressed rClfA truncate rClfA40‐559 (Josefsson et al., 2001).
- Detailed Gene Information: Click here.
|
f. Adjuvant: |
|
g. Immunization Route |
subcutaneous injection |
h.
Mouse Response |
- Host Strain:
NMRI
- Vaccination Protocol:
Purified rClfA region A, comprising residues 40–559 (rClfA40‐559), and BSA (Sigma Chemical) were dissolved in physiologic saline and were emulsified 1:1 in Freund's complete adjuvant (Difco Laboratories). In total, 200 μL of the emulsion containing 30 μg of protein was injected subcutaneously (sc) on day −21 ( /group). Booster immunization with 30 μg of protein in physiologic saline:Freund's incomplete adjuvant 1:1 sc was done on day −10 (Josefsson et al., 2001).
- Challenge Protocol:
On day 0, the mice were challenged iv with 1.6 x 10^7 cfu/mouse of wild‐type S. aureus strain Newman (Josefsson et al., 2001).
- Efficacy:
Mice immunized with rClfA40‐559 had less severe arthritis than did the control BSA‐immunized group throughout the experiment. Both the severity of synovitis and the extent of joint destruction were markedly less pronounced in mice immunized with rClfA40‐559 than in the BSA control group (Josefsson et al., 2001).
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
2. S. aureus CNA Protein Vaccine |
a. Vaccine Ontology ID: |
VO_0004048 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Antigen |
a recombinant fragment of CNA protein (Nilsson et al., 1998). |
e. Gene Engineering of
cna |
- Type:
Recombinant protein preparation
- Description:
CNA fragments were expressed in E. coli and then purified (Nilsson et al., 1998).
- Detailed Gene Information: Click here.
|
f. Adjuvant: |
|
g. Immunization Route |
subcutaneous injection |
h.
Mouse Response |
- Host Strain:
NMRI
- Vaccination Protocol:
Purified recombinant collagen adhesin fragments M17, M31, M55, and BSA (Sigma Chemical Co.) were dissolved in PBS and emulsified 1:1 in Freund’s complete adjuvant 200 μl of the emulsion containing 100 μg of protein was injected subcutaneously (s.c.) on day -31. Booster immunizations (s.c.; 100 μg protein in PBS) were performed 15 and 24 d after the initial vaccination (Nilsson et al., 1998).
- Challenge Protocol:
After the final boost, mice were inoculated intravenously (i.v.) with S. aureus (Nilsson et al., 1998).
- Efficacy:
At 14 d after inoculation, mortality in the collagen adhesin-vaccinated group was only 13%, compared with 87% in the control antigen immunized group (Nilsson et al., 1998).
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
3. S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna |
a. Vaccine Ontology ID: |
VO_0004542 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Mouse |
e. Antigen |
fibrinogen binding protein (Efb), fibronectin-binding protein A (FnbpA), clumping factor A (ClfA) and collagen-binding protein (Cna) from S. aureus strain 1706 (Castagliuolo et al., 2006) |
f. Gene Engineering of
ClfA |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
|
g. Gene Engineering of
cna |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
|
h. Gene Engineering of
efb |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
|
i. Gene Engineering of
fnbA |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
|
j. Vector: |
pcDNA3.1 (Castagliuolo et al., 2006) |
k. Immunization Route |
intranasal immunization |
l.
Mouse Response |
- Vaccine Immune Response Type:
VO_0000286
- Immune Response:
Intranasal immunization with a pDNA mixture coding the four adhesins triggered significant levels of specific serum and mucosal Ig that inhibited S. aureus adhesion to cow mammary gland epithelial cells in vitro. Splenocytes of immunized mice challenged in vitro with S. aureus extracts showed a strong proliferative response (Castagliuolo et al., 2006).
- Efficacy:
In non immune mice or mice injected with empty vectors, a large number of bacteria was recovered from the mammary glands. Whereas, in mice immunized with the genetic vaccine coding the four S. aureus adhesins, the amount of bacteria recovered from the mammary glands was significantly decreased (Castagliuolo et al., 2006).
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4. S. aureus DNA Vaccine encoding PBP2a Protein |
a. Vaccine Ontology ID: |
VO_0004192 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Gene Engineering of
MecA |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
|
e. Vector: |
pCI-Neo Mammalian Expression Vector (Promega) |
f. Immunization Route |
Intramuscular injection (i.m.) |
g.
Mouse Response |
- Host Strain:
BALB/c
- Vaccination Protocol:
Three days before the first immunization, bupivacain chloridrate 0.5% was injected at a dose of 2.5 μl/g of animal weight in the left quadriceps muscle. For DNA vaccination, three intramuscular injections with 10 μg of plasmid pCI-Neo-mecA or pCI-Neo only (negative control) diluted in 50 μl of sterile PBS were given at 2 weeks intervals into the left quadriceps muscle of mice (Senna et al., 2003).
- Challenge Protocol:
To assess the immune response against MRSA, mice immunized with pCI-Neo and pCI-Neo-mecA were challenged with MRSA (MRSA HPS-03). Mice received a sublethal dose of 2.0×10^6 cfu intraperitonially daily for 7 days. A non-immunized group receiving the same bacterial dose was included as positive control.
- Efficacy:
After challenge, the number of bacteria from kidneys from immunized and non-immunized mice was determined. Kidneys from immunized mice had 1000 times less on bacteria than the positive controls (non-immunized mice) (Senna et al., 2003).
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
5. S. aureus DNA vaccine pClfaSrtD13 |
a. Vaccine Ontology ID: |
VO_0004541 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Mouse |
e. Antigen |
Clumping factor A (Clfa), fibronectin binding protein A (FnBPA) and the enzyme Sortase (Srt) from strains “Newman” or “8325-4” (Gaudreau et al., 2007) |
f. Gene Engineering of
ClfA |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
|
g. Vector: |
pCI (Gaudreau et al., 2007) |
h. Immunization Route |
Intramuscular injection (i.m.) |
i.
Mouse Response |
- Vaccine Immune Response Type:
VO_0000286
- Immune Response:
All animals produced a mixed Th1 and Th2 response including functional antigen-specific, mostly IgG2a antibodies, sustained production of IFN-gamma and a predominantly CD8+ T-cell response (Gaudreau et al., 2007).
- Efficacy:
Fourteen days after challenge, 70% of the vaccinated mice had survived as compared to only 15% of the control mice and after 21 days, the figures were 55% survival for the vaccinated as compared to 15% survival of the control group. However, signs of arthritis were observed in all the infected mice whether vaccinated or not (Gaudreau et al., 2007).
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
6. S. aureus FnbA Protein Vaccine |
a. Vaccine Ontology ID: |
VO_0004049 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Antigen |
Fibronectin binding domain of FnbA protein |
e. Gene Engineering of
fnbA |
- Type:
Recombinant protein preparation
- Description:
- Detailed Gene Information: Click here.
|
f. Adjuvant: |
|
g. Immunization Route |
subcutaneous injection |
h.
Mouse Response |
- Host Strain:
BSVS
- Vaccination Protocol:
Mice were vaccinated subcutaneously in the neck region with 20 μg of the antigens emulsified with complete Freund's adjuvant (primary vaccination) a few days after mating. Booster doses were given after 14 days with the same amount of immunogen, but emulsified with incomplete Freund's adjuvant (Mamo et al., 1994).
- Challenge Protocol:
Mice were challenged 18-21 days after the booster dose by intramammary inoculation (mouse mastitis model) 23. Briefly, mice were anaesthetized using ether inhalation, the teat tips were aseptically removed and a 0.1 ml bacterial suspension (1 x l0^6 c.f.u, m1-1) was inoculated into the left quarter (L-4) and right quarter (R-4) mouse mammary glands (Mamo et al., 1994).
- Efficacy:
Vaccination of mice with recombinant FnBP resulted in significant protection against challenge with S. aureus (Mamo et al., 1994).
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
7. S. aureus IsdB subunit vaccine (V710) |
a. Product Name: |
V710 |
b. Vaccine Ontology ID: |
VO_0004220 |
c. Type: |
Subunit vaccine |
d. Status: |
Research |
e. Antigen |
Purified IsdB protein |
f. Adjuvant: |
|
g. Immunization Route |
Intramuscular injection (i.m.) |
h.
Mouse Response |
- Host Strain:
BALB/c
- Vaccination Protocol:
Groups of 20 BALB/c or ICR mice were immunized three times with IsdB (20 μg per dose) formulated with amorphous aluminum hydroxyphosphate sulfate adjuvant (AAHSA) (450 μg per dose) or were sham immunized with AAHSA alone. The doses were administered as two 50-μl intramuscular injections on days 0, 7, and 21(Kuklin et al., 2006).
- Challenge Protocol:
35 days after vaccination, mice were challenged with an 80 to 90% lethal dose (LD80-90) of S. aureus Becker (4.9 × 10^8 to 8.7 × 10^8 CFU for BALB/c mice and 1.0 × 10^9 to 2.0 × 10^9 CFU for ICR mice), and survival was monitored for 10 days. (Kuklin et al., 2006).
- Efficacy:
Mice immunized with IsdB exhibited greater survival (45, 40, 32, 29, 40, and 40%) than the mice in the sham-immunized control group (Kuklin et al., 2006).
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
8. Staphylococcus aureus aroA mutant vaccine |
a. Vaccine Ontology ID: |
VO_0002797 |
b. Type: |
Live, attenuated vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Mouse |
e. Gene Engineering of
aroA |
|
f. Immunization Route |
Intramammary immunization |
g.
Mouse Response |
- Persistence:
An aroA mutant is attenuated in mice (Buzzola et al., 2006).
- Efficacy:
An aroA mutant induces significant protection in mice from challenge with wild type S. aureus (Buzzola et al., 2006).
- Host Gene Response of
Ifng (Interferon gamma)
- Gene Response:
Ninety-six hours after challenge with the wild-type strain RN6390 or heterologous strain MB319, the relative mRNA levels of IFN-γ and IL-4 were determined in mammary glands by RT-PCR. Mammary glands from mice immunized with the aroA mutant showed a significant increase in the level of IFN-γ transcripts compared with control, unvaccinated mice (Buzzola et al., 2006).
- Detailed Gene Information: Click here.
- Host Gene Response of
Il4 (interleukin 4)
- Gene Response:
Ninety-six hours after challenge with the wild-type strain RN6390 or heterologous strain MB319, the relative mRNA levels of IFN-γ and IL-4 were determined in mammary glands by RT-PCR. Mice immunized with the aroA mutant showed a significant increase in the level of IL-4 transcripts compared with control, unvaccinated mice (Buzzola et al., 2006).
- Detailed Gene Information: Click here.
|
|
|
|
|
|
|
|
|
V. References |
1. Bagnoli et al., 2015: Bagnoli F, Fontana MR, Soldaini E, Mishra RP, Fiaschi L, Cartocci E, Nardi-Dei V, Ruggiero P, Nosari S, De Falco MG, Lofano G, Marchi S, Galletti B, Mariotti P, Bacconi M, Torre A, Maccari S, Scarselli M, Rinaudo CD, Inoshima N, Savino S, Mori E, Rossi-Paccani S, Baudner B, Pallaoro M, Swennen E, Petracca R, Brettoni C, Liberatori S, Norais N, Monaci E, Bubeck Wardenburg J, Schneewind O, O'Hagan DT, Valiante NM, Bensi G, Bertholet S, De Gregorio E, Rappuoli R, Grandi G. Vaccine composition formulated with a novel TLR7-dependent adjuvant induces high and broad protection against Staphylococcus aureus. Proceedings of the National Academy of Sciences of the United States of America. 2015; 112(12); 3680-3685. [PubMed: 25775551].
2. Buzzola et al., 2006: Buzzola FR, Barbagelata MS, Caccuri RL, Sordelli DO. Attenuation and persistence of and ability to induce protective immunity to a Staphylococcus aureus aroA mutant in mice. Infection and immunity. 2006; 74(6); 3498-3506. [PubMed: 16714581].
3. Castagliuolo et al., 2006: Castagliuolo I, Piccinini R, Beggiao E, Palù G, Mengoli C, Ditadi F, Vicenzoni G, Zecconi A. Mucosal genetic immunization against four adhesins protects against Staphylococcus aureus-induced mastitis in mice. Vaccine. 2006; 24(20); 4393-4402. [PubMed: 16580097].
4. Clarke et al., 2006: Clarke SR, Brummell KJ, Horsburgh MJ, McDowell PW, Mohamad SA, Stapleton MR, Acevedo J, Read RC, Day NP, Peacock SJ, Mond JJ, Kokai-Kun JF, Foster SJ. Identification of in vivo-expressed antigens of Staphylococcus aureus and their use in vaccinations for protection against nasal carriage. The Journal of infectious diseases. 2006; 193(8); 1098-1108. [PubMed: 16544250].
5. Cui et al., 2005: Cui JC, Hu DL, Lin YC, Qian AD, Nakane A. Immunization with glutathione S-transferase and mutant toxic shock syndrome toxin 1 fusion protein protects against Staphylococcus aureus infection. FEMS immunology and medical microbiology. 2005; 45(1); 45-51. [PubMed: 15985222].
6. Delfani et al., 2016: Delfani S, Mohabati Mobarez A, Imani Fooladi AA, Amani J, Emaneini M. Protection of mice against Staphylococcus aureus infection by a recombinant protein ClfA-IsdB-Hlg as a vaccine candidate. Medical microbiology and immunology. 2016; 205(1); 47-55. [PubMed: 26155981].
7. Gaudreau et al., 2007: Gaudreau MC, Lacasse P, Talbot BG. Protective immune responses to a multi-gene DNA vaccine against Staphylococcus aureus. Vaccine. 2007; 25(5); 814-824. [PubMed: 17027124].
8. Harro et al., 2010: Harro C, Betts R, Orenstein W, Kwak EJ, Greenberg HE, Onorato MT, Hartzel J, Lipka J, DiNubile MJ, Kartsonis N. Safety and immunogenicity of a novel Staphylococcus aureus vaccine: results from the first study of the vaccine dose range in humans. Clinical and vaccine immunology : CVI. 2010; 17(12); 1868-1874. [PubMed: 20943877].
9. Josefsson et al., 2001: Josefsson E, Hartford O, O'Brien L, Patti JM, Foster T. Protection against experimental Staphylococcus aureus arthritis by vaccination with clumping factor A, a novel virulence determinant. The Journal of infectious diseases. 2001; 184(12); 1572-1580. [PubMed: 11740733].
10. Karauzum et al., 2013: Karauzum H, Adhikari RP, Sarwar J, Devi VS, Abaandou L, Haudenschild C, Mahmoudieh M, Boroun AR, Vu H, Nguyen T, Warfield KL, Shulenin S, Aman MJ. Structurally designed attenuated subunit vaccines for S. aureus LukS-PV and LukF-PV confer protection in a mouse bacteremia model. PloS one. 2013; 8(6); e65384. [PubMed: 23762356].
11. Kuipers et al., 2016: Kuipers A, Stapels DA, Weerwind LT, Ko YP, Ruyken M, Lee JC, van Kessel KP, Rooijakkers SH. The Staphylococcus aureus polysaccharide capsule and Efb-dependent fibrinogen shield act in concert to protect against phagocytosis. Microbiology (Reading, England). 2016; 162(7); 1185-1194. [PubMed: 27112346].
12. Kuklin et al., 2006: Kuklin NA, Clark DJ, Secore S, Cook J, Cope LD, McNeely T, Noble L, Brown MJ, Zorman JK, Wang XM, Pancari G, Fan H, Isett K, Burgess B, Bryan J, Brownlow M, George H, Meinz M, Liddell ME, Kelly R, Schultz L, Montgomery D, Onishi J, Losada M, Martin M, Ebert T, Tan CY, Schofield TL, Nagy E, Meineke A, Joyce JG, Kurtz MB, Caulfield MJ, Jansen KU, McClements W, Anderson AS. A novel Staphylococcus aureus vaccine: iron surface determinant B induces rapid antibody responses in rhesus macaques and specific increased survival in a murine S. aureus sepsis model. Infection and immunity. 2006; 74(4); 2215-2223. [PubMed: 16552052].
13. Lacey et al., 2017: Lacey KA, Leech JM, Lalor SJ, McCormack N, Geoghegan JA, McLoughlin RM. The Staphylococcus aureus Cell Wall-Anchored Protein Clumping Factor A Is an Important T Cell Antigen. Infection and immunity. 2017; 85(12); . [PubMed: 28947645].
14. Mamo et al., 1994: Mamo W, Jonsson P, Flock JI, Lindberg M, Müller HP, Wadström T, Nelson L. Vaccination against Staphylococcus aureus mastitis: immunological response of mice vaccinated with fibronectin-binding protein (FnBP-A) to challenge with S. aureus. Vaccine. 1994; 12(11); 988-992. [PubMed: 7975852].
15. Mamo et al., 2000: Mamo W, Frman G, Mller HP. Protection induced in mice vaccinated with recombinant collagen-binding protein (CnBP) and alpha-toxoid against intramammary infection with Staphylococcus aureus. Microbiology and immunology. 2000; 44(5); 381-384. [PubMed: 10888356].
16. Narita et al., 2015: Narita K, Hu DL, Asano K, Nakane A. Vaccination with non-toxic mutant toxic shock syndrome toxin-1 induces IL-17-dependent protection against Staphylococcus aureus infection. Pathogens and disease. 2015; 73(4); . [PubMed: 25857736].
17. Nilsson et al., 1998: Nilsson IM, Patti JM, Bremell T, Höök M, Tarkowski A. Vaccination with a recombinant fragment of collagen adhesin provides protection against Staphylococcus aureus-mediated septic death. The Journal of clinical investigation. 1998; 101(12); 2640-2649. [PubMed: 9637697].
18. Nilsson et al., 1999: Nilsson IM, Verdrengh M, Ulrich RG, Bavari S, Tarkowski A. Protection against Staphylococcus aureus sepsis by vaccination with recombinant staphylococcal enterotoxin A devoid of superantigenicity. The Journal of infectious diseases. 1999; 180(4); 1370-1373. [PubMed: 10479175].
19. Pozzi et al., 2015: Pozzi C, Lofano G, Mancini F, Soldaini E, Speziale P, De Gregorio E, Rappuoli R, Bertholet S, Grandi G, Bagnoli F. Phagocyte subsets and lymphocyte clonal deletion behind ineffective immune response to Staphylococcus aureus. FEMS microbiology reviews. 2015; 39(5); 750-763. [PubMed: 25994610].
20. Rauch et al., 2014: Rauch S, Gough P, Kim HK, Schneewind O, Missiakas D. Vaccine protection of leukopenic mice against Staphylococcus aureus bloodstream infection. Infection and immunity. 2014; 82(11); 4889-4898. [PubMed: 25183728].
21. Reddy et al., 2015: Reddy PN, Paul S, Sripathy MH, Batra HV. Evaluation of recombinant SEA-TSST fusion toxoid for protection against superantigen induced toxicity in mouse model. Toxicon : official journal of the International Society on Toxinology. 2015; 103; 106-113. [PubMed: 26091873].
22. Senna et al., 2003: Senna JP, Roth DM, Oliveira JS, Machado DC, Santos DS. Protective immune response against methicillin resistant Staphylococcus aureus in a murine model using a DNA vaccine approach. Vaccine. 2003; 21(19-20); 2661-2666. [PubMed: 12744903].
23. Stranger-Jones et al., 2006: Stranger-Jones YK, Bae T, Schneewind O. Vaccine assembly from surface proteins of Staphylococcus aureus. Proceedings of the National Academy of Sciences of the United States of America. 2006; 103(45); 16942-16947. [PubMed: 17075065].
24. Veloso et al., 2015: Veloso TR, Mancini S, Giddey M, Vouillamoz J, Que YA, Moreillon P, Entenza JM. Vaccination against Staphylococcus aureus experimental endocarditis using recombinant Lactococcus lactis expressing ClfA or FnbpA. Vaccine. 2015; 33(30); 3512-3517. [PubMed: 26048778].
25. Wiki: S. aureus: Wiki: Staphylococcus aureus [http://en.wikipedia.org/wiki/Staphylococcus_aureus]
26. Yang et al., 2016: Yang HJ, Zhang JY, Wei C, Yang LY, Zuo QF, Zhuang Y, Feng YJ, Srinivas S, Zeng H, Zou QM. Immunisation With Immunodominant Linear B Cell Epitopes Vaccine of Manganese Transport Protein C Confers Protection against Staphylococcus aureus Infection. PloS one. 2016; 11(2); e0149638. [PubMed: 26895191].
27. Yang et al., 2017: Yang Y, Yu R, Yang X, Liu S, Fang T, Song X, Hou L, Yu C, Xu J, Fu L, Yi S, Chen W. Protection against Staphylococcus aureus and tetanus infections by a combined vaccine containing SasA and TeNT?Hc in mice. Molecular medicine reports. 2017; 15(4); 2369-2373. [PubMed: 28259925].
28. Yu et al., 2014: Yu L, Fan Z, Ma J, Tong C, Song B, Zhu Z, Cui Y. Cross-protective effect of a novel multi-antigen-chimeric vaccine against Streptococcus and Staphylococcus aureus infection in mice. Journal of medical microbiology. 2014; 63(Pt 12); 1732-1740. [PubMed: 25288644].
29. Yu et al., 2015: Yu S, Zhang H, Yao D, Liu W, Wang X, Chen X, Wei Y, Zhang Z, Wang J, Yu L, Sun H, Wu Z, Yu Y, Song B, Ma J, Tong C, Cui Y. Identification of CD4+ T-cell epitopes on iron-regulated surface determinant B of Staphylococcus aureus. Microbial pathogenesis. 2015; 89; 108-113. [PubMed: 26423555].
30. Zhao et al., 2014: Zhao Z, Li B, Sun HQ, Zhang JY, Wang YL, Chen L, Hu J, He YF, Zeng H, Zou QM, Wu C. Fine-mapping of immunodominant linear B-cell epitopes of the Staphylococcus aureus SEB antigen using short overlapping peptides. PloS one. 2014; 9(3); e90445. [PubMed: 24599257].
|
|