|
Pseudomonas aeruginosa |
Table of Contents |
- General Information
- NCBI Taxonomy ID
- Disease
- Introduction
- Microbial Pathogenesis
- Host Ranges and Animal Models
- Host Protective Immunity
- Vaccine Related Pathogen Genes
- algE
(Protective antigen)
- flagellin
(Protective antigen)
- fliC
(Protective antigen)
- fliC
(Protective antigen)
- FliD
(Protective antigen)
- hcp1
(Protective antigen)
- OprF
(Protective antigen)
- oprF_3
(Protective antigen)
- OprI
(Protective antigen)
- oprL
(Protective antigen)
- PcrV
(Protective antigen)
- pilA
(Protective antigen)
- pilA
(Protective antigen)
- pilA
(Protective antigen)
- popB
(Protective antigen)
- Vaccine Information
- Mink Distemper-Enteritis Modified Live & Killed Virus Vaccine-Clostridium Botulinum Type C-Pseudomonas Aeruginosa Bacterin-Toxoid (USDA: 4949.20)
- Mink Distemper-Enteritis Modified Live & Killed Virus Vaccine-Clostridium Botulinum Type C-Pseudomonas Aeruginosa Bacterin-Toxoid (USDA: 4949.31)
- Mink Enteritis Killed Virus Vaccine-Clostridium Botulinum Type C-Pseudomonas Aeruginosa Bacterin-Toxoid (USDA: 49A5.20)
- Mink Enteritis Killed Virus Vaccine-Clostridium Botulinum Type C-Pseudomonas Aeruginosa Bacterin-Toxoid (USDA: 49A5.21)
- P. aeruginosa DNA Vaccine encoding OprF
- P. aeruginosa DNA vaccine pGACAG-OprF/OprI
- P. aeruginosa DNA vaccine pGACAG-OprF/OprI + pGACAG-PcrV + pGACAG-PilA
- P. aeruginosa DNA vaccine pGACAG-PcrV
- P. aeruginosa OprI Protein Vaccine
- P. aeruginosa PcrV Protein Vaccine
- SL3261-P. aeruginosa serogroup O11 O antigen
- References
|
I. General Information |
1. NCBI Taxonomy ID: |
287 |
2. Disease: |
Pseudomonas aeruginosa infection |
3. Introduction |
Pseudomonas aeruginosa is member of the Gamma Proteobacteria class of Bacteria. It is a Gram-negative, aerobic rod belonging to the bacterial family Pseudomonadaceae. Since the revisionist taxonomy based on conserved macromolecules (e.g. 16S ribosomal RNA) the family includes only members of the genus Pseudomonas which are cleaved into eight groups. Pseudomonas aeruginosa is the type species of its group. which contains 12 other members. Pseudomonas aeruginosa is an opportunistic pathogen, meaning that it exploits some break in the host defenses to initiate an infection. In fact, Pseudomonas aeruginosa is the epitome of an opportunistic pathogen of humans. The bacterium almost never infects uncompromised tissues, yet there is hardly any tissue that it cannot infect if the tissue defenses are compromised in some manner. It causes urinary tract infections, respiratory system infections, dermatitis, soft tissue infections, bacteremia, bone and joint infections, gastrointestinal infections and a variety of systemic infections, particularly in patients with severe burns and in cancer and AIDS patients who are immunosuppressed. Pseudomonas aeruginosa infection is a serious problem in patients hospitalized with cancer, cystic fibrosis, and burns. The case fatality rate in these patients is near 50 percent (Textbook of Bacteriology). |
4. Microbial Pathogenesis |
P. aeruginosa infections are widely varied. The bacteria can colonize wounds and enter the blood stream, they can colonize the lungs, and they can also colonize the a scratched cornea. P. aeruginosa has a variety of virulence factors that contribute to its widely varied pathogenesis (Salyers and Whitt., 2002). |
5. Host Ranges and Animal Models |
Pseudomonas aeruginosa is an opportunistic human pathogen. The burned mouse model is used to model pseudomonas infection in burn patients. For lung infections guinea pig and rat models are used. C. elegans is also a model used to study host-pathogen interactions (Salyers and Whitt., 2002). |
6. Host Protective Immunity |
Most strains of P. aeruginosaare resistant to killing in serum alone, but the addition of polymorphonuclear leukocytes results in bacterial killing. Killing is most efficient in the presence of type-specific opsonizing antibodies, directed primarily at the antigenic determinants of LPS. This suggests that phagocytosis is an important defense and that opsonizing antibody is the principal functional antibody in protecting from P. aeruginosa infections. Once P. aeruginosa infection is established, other antibodies, such as antitoxin, may be important in controlling disease. Cell-mediated immunity does not seem to play a major role in resistance or defense against Pseudomonas infections (Textbook of Bacteriology). |
II. Vaccine Related Pathogen Genes |
1. algE |
-
Gene Name :
algE
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa
-
NCBI Protein GI :
NP_252234
-
Other Database IDs :
CDD:315937
-
Taxonomy ID :
208964
-
Gene Strand (Orientation) :
?
-
Protein Name :
alginate production protein AlgE
-
Protein pI :
4.64
-
Protein Weight :
53211.29
-
Protein Length :
574
-
Protein Note :
Alginate export; pfam13372
-
Protein Sequence : Show Sequence
>NP_252234.1 alginate production protein AlgE [Pseudomonas aeruginosa PAO1]
MNSSRSVNPRPSFAPRALSLAIALLLGAPAFAANSGEAPKNFGLDVKITGESENDRDLGTAPGGTLNDIG
IDLRPWAFGQWGDWSAYFMGQAVAATDTIETDTLQSDTDDGNNSRNDGREPDKSYLAAREFWVDYAGLTA
YPGEHLRFGRQRLREDSGQWQDTNIEALNWSFETTLLNAHAGVAQRFSEYRTDLDELAPEDKDRTHVFGD
ISTQWAPHHRIGVRIHHADDSGHLRRPGEEVDNLDKTYTGQLTWLGIEATGDAYNYRSSMPLNYWASATW
LTGDRDNLTTTTVDDRRIATGKQSGDVNAFGVDLGLRWNIDEQWKAGVGYARGSGGGKDGEEQFQQTGLE
SNRSNFTGTRSRVHRFGEAFRGELSNLQAATLFGSWQLREDYDASLVYHKFWRVDDDSDIGTSGINAALQ
PGEKDIGQELDLVVTKYFKQGLLPASMSQYVDEPSALIRFRGGLFKPGDAYGPGTDSTMHRAFVDFIWRF
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Furthermore, we suggest to include AlgE in a multicomponent experimental vaccine for potential protection of non-colonized CF patients from colonization with mucoid P. aeruginosa.(Rehm et al., 1994)
|
2. flagellin |
-
Gene Name :
flagellin
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa
-
NCBI Protein GI :
CAA67105
-
Other Database IDs :
CDD:279061
CDD:284584 CDD:224263 CDD:279090 GOA:P72127 HSSP: 1ORY InterPro: IPR001029 InterPro: IPR001492 InterPro: IPR010810 UniProtKB/TrEMBL: P72127
-
Taxonomy ID :
287
-
Protein Name :
flagellin
-
Protein pI :
4.36
-
Protein Weight :
67085.52
-
Protein Length :
767
-
Protein Note :
Bacterial flagellin N-terminal helical region; pfam00669
-
Protein Sequence : Show Sequence
>CAA67105.1 flagellin, partial [Pseudomonas aeruginosa]
GLQIANRLTSQITGVNTAVKNANDGISIAQTAEGALQESTNILQRMRELSLQAANGSNSDEDRNSLNQEF
SALSKELTRIGSTTTFDTGMKLLDGSAGTLSFQVGANANENISFSLGKMDAASLKSTSDTASVVGGPQPN
SVLTSTLTQSASVVSTALVGTGSGSLGNVSINGVGVDLSSIADDDGAAAAKAINDSKAGVTATWDATAKT
ISLSSNKDIKLADVDAGALAKIGLTAGTTDAQISKTATQVGTALAGSGTANFADMAINGSSVALSSIKDN
DGAAAVKAINDAKISGVSASRDATTKKITLSSENSDLKVDGAAAGDLEKLGLGAASDTTKTTTAVIDYDD
VSINGHPIALNGKTSASDVVQEINKWTSNTGVTASIDDSGKLSLASKNGKIDFGNTNSLALDKLGVSSSA
KPTFALEKDTSFVLNGNEIKLSKGDSLDAVAQKINSAGLGVGAAVDNGKLVLNSYGADITLTDGGDKADK
WWAGSYRSDQGTTSATTTHAQGNVDLSNQGLAADGSININGTDISFKAGDKIDDVVSKINEQSQATGVSA
AKDAHGLLVLNSNSDFTLGVPTGAGATDATAALGLTAGTSIAQETSVAELDIKTAFDAQKSIQVIDSALK
QIDSQRAELGAVQNRFDSTISNLQSISENATSARSRVQDTDFAAETAEMTKQQTLQQASTAVLAQANQLP
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Laghaei et al., 2016)
|
3. fliC |
-
Gene Name :
fliC
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa
-
NCBI Protein GI :
AAP34195
-
Other Database IDs :
CDD:236344
-
Taxonomy ID :
287
-
Protein Name :
FliC
-
Protein pI :
4.37
-
Protein Weight :
11293.39
-
Protein Length :
179
-
Protein Note :
flagellin; Reviewed
-
Protein Sequence : Show Sequence
>AAP34195.1 FliC, partial [Pseudomonas aeruginosa]
NVKVDMKGNETAEQAAAKIAAAVNDANVGIGAFSDGDTISYVSKAGKDGSGAITSAVSGVVIADTGSTGV
GTAAGVAPSATAFAKTNDTVAKIDISTAKGAQSAVLVIDEAIKQIDAQRADLGAV
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
|
4. fliC |
-
Gene Name :
fliC
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa
-
NCBI Protein GI :
AAB69135
-
Other Database IDs :
CDD:279061
CDD:284584 CDD:279090
-
Taxonomy ID :
287
-
Protein Name :
flagellin
-
Protein pI :
4.82
-
Protein Weight :
40421.39
-
Protein Length :
498
-
Protein Note :
Bacterial flagellin N-terminal helical region; pfam00669
-
Protein Sequence : Show Sequence
>AAB69135.1 flagellin, partial [Pseudomonas aeruginosa]
GLQISNRLSNQISGLNVATRNANDGISLAQTAEGALQQSTNILQRIRDLALQSANGSNSDADRAALQKEV
AAQQAELTRISDTTTFGGRKLLDGSFGTTSFQVGSNAYETIDISLQNASASAIGSYQVGSNGAGTVASVA
GTATASGIASGTVNLVGGGQVKNIAIAAGDSAKAIAEKMDGAIPNLSARARTVFTADVSGVTGGSLNFDV
TVGSNTVSLAGVTSTQDLADQLNSNSSKLGNTASINDKGVLTITSATGENVKFGAQTGTATAGQVAVKVQ
GSDGKFEAAAKNVVAAGTAATTTIVTGYVQLNSPTAYSVSGTGTQASQVFGNASAAQKSSVASVDISTAD
GAQNAIAVVDNALAAIDAQRADLSAVQNRFKNTIDNLTNISENATNARSRIKDTDFAAETAALSKNQVLQ
QAGTAILAQANQLP
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Behrouz et al., 2016)
|
5. FliD |
-
Gene Name :
FliD
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa
-
NCBI Protein GI :
EAZ56903
-
Other Database IDs :
CDD:224264
-
Taxonomy ID :
350703
-
Protein Name :
flagellar cap; FliD
-
Protein pI :
7.1
-
Protein Weight :
47678.93
-
Protein Length :
548
-
Protein Note :
Flagellar capping protein FliD [Cell motility]; COG1345
-
Protein Sequence : Show Sequence
>EAZ56903.1 flagellar cap; FliD [Pseudomonas aeruginosa 2192]
MANSTTINGYNSGLDIKNIVSTLVAAEKAPKEAQLKRLESDTTAKFTGIGQLKSAISDLQTILKELNKPE
LFQKRSASTSDEKFATATATKDALPGIYKLEVTQLASVSKVATASFADGYKTTSGGTLTIKQGADDAGVT
VNVAAGATLAEVRDSLNAQLKDKGITANIVNNPGDGTSRLVFTGKDSGAGKDVFVQGSSGLENFNIGSVG
ADGKLTLSQLDGSSSSSSGYITQAKNAKFSIDGLTLESPTNTVDKVINGVTFELKTVTDTNKPITISVEQ
DRGGVKDNIKKFVEAYNKLVGVTSELTGVTKVGDDKAPVVGALVGDSSVRNLLTTMRNEMVQPGQGTDVR
MLADMGITTKKDGTLEIDDKKLDKVLKDKFESVSALFTGDTGLMKRLDDKLTPYTQTGGVLQQRLDGLQD
TIKSVDTQREALNRRVEQLQDRLLKQFTAMDQLIGQLNQTSGRMAQALSSLPGLVKKS
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
|
6. hcp1 |
-
Gene Name :
hcp1
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa
-
NCBI Protein GI :
ARG84223
-
Other Database IDs :
CDD:310322
-
Taxonomy ID :
287
-
Protein Name :
Hcp1
-
Protein pI :
7.66
-
Protein Weight :
16544.36
-
Protein Length :
208
-
Protein Note :
Type VI secretion system effector, Hcp; pfam05638
-
Protein Sequence : Show Sequence
>ARG84223.1 Hcp1 [Pseudomonas aeruginosa]
MAVDMFIKIGDVKGESKDKTHAEEIDVLAWSWGMSQSGSMHMGGGGGAGKVNVQDLSFTKYIDKSTPNLM
MACSSGKHYPQAKLTIRKAGGENQVEYLIITLKEVLVSSVSTGGSGGEDRLTENVTLNFAQVQVDYQPQK
ADGAKDGGPVKYGWNIRQNVQA
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Yang et al., 2017)
|
7. OprF |
-
Gene Name :
OprF
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa PAO1
-
VO ID :
VO_0012360
-
NCBI Gene ID :
878442
-
NCBI Protein GI :
15596974
-
Locus Tag :
PA1777
-
Genbank Accession :
AE004091
-
Protein Accession :
NP_250468
-
Taxonomy ID :
208964
-
Gene Starting Position :
1921173
-
Gene Ending Position :
1922225
-
Gene Strand (Orientation) :
+
-
Protein Name :
outer membrane porin F
-
Protein pI :
4.75
-
Protein Weight :
34266.56
-
Protein Length :
350
-
DNA Sequence : Show Sequence
>NC_002516.2:1921173-1922225 Pseudomonas aeruginosa PAO1 chromosome, complete genome
GATGAAACTGAAGAACACCTTAGGCGTTGTCATCGGCTCGCTGGTTGCCGCTTCGGCAATGAACGCCTTT
GCCCAGGGCCAGAACTCGGTAGAGATCGAAGCCTTCGGCAAGCGCTACTTCACCGACAGCGTTCGCAACA
TGAAGAACGCGGACCTGTACGGCGGCTCGATCGGTTACTTCCTGACCGACGACGTCGAGCTGGCGCTGTC
CTACGGTGAGTACCATGACGTTCGTGGCACCTACGAAACCGGCAACAAGAAGGTCCACGGCAACCTGACC
TCCCTGGACGCCATCTACCACTTCGGTACCCCGGGCGTAGGTCTGCGTCCGTACGTGTCGGCTGGTCTGG
CTCACCAGAACATCACCAACATCAACAGCGACAGCCAAGGCCGTCAGCAGATGACCATGGCCAACATCGG
CGCTGGTCTGAAGTACTACTTCACCGAGAACTTCTTCGCCAAGGCCAGCCTCGACGGCCAGTACGGTCTG
GAGAAGCGTGACAACGGTCACCAGGGCGAGTGGATGGCTGGCCTGGGCGTCGGCTTCAACTTCGGTGGTT
CGAAAGCCGCTCCGGCTCCGGAACCGGTTGCCGACGTTTGCTCCGACTCCGACAACGACGGCGTTTGCGA
CAACGTCGACAAGTGCCCGGATACCCCGGCCAACGTCACCGTTGACGCCAACGGCTGCCCGGCTGTCGCC
GAAGTCGTACGCGTACAGCTGGACGTGAAGTTCGACTTCGACAAGTCCAAGGTCAAAGAGAACAGCTACG
CTGACATCAAGAACCTGGCTGACTTCATGAAGCAGTACCCGTCCACTTCCACCACCGTTGAAGGTCACAC
CGACTCCGTCGGCACCGACGCTTACAACCAGAAGCTGTCCGAGCGTCGTGCCAACGCCGTTCGTGACGTA
CTGGTCAACGAGTACGGTGTAGAAGGTGGTCGCGTGAACGCTGTTGGTTACGGCGAGTCCCGCCCGGTTG
CCGACAACGCCACCGCTGAAGGCCGCGCTATCAACCGTCGCGTTGAAGCCGAAGTAGAAGCTGAAGCCAA
GTA
-
Protein Sequence : Show Sequence
>NP_250468.1 outer membrane porin F [Pseudomonas aeruginosa PAO1]
MKLKNTLGVVIGSLVAASAMNAFAQGQNSVEIEAFGKRYFTDSVRNMKNADLYGGSIGYFLTDDVELALS
YGEYHDVRGTYETGNKKVHGNLTSLDAIYHFGTPGVGLRPYVSAGLAHQNITNINSDSQGRQQMTMANIG
AGLKYYFTENFFAKASLDGQYGLEKRDNGHQGEWMAGLGVGFNFGGSKAAPAPEPVADVCSDSDNDGVCD
NVDKCPDTPANVTVDANGCPAVAEVVRVQLDVKFDFDKSKVKENSYADIKNLADFMKQYPSTSTTVEGHT
DSVGTDAYNQKLSERRANAVRDVLVNEYGVEGGRVNAVGYGESRPVADNATAEGRAINRRVEAEVEAEAK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
The oprF gene was cloned into plasmid vector pVR1020, and the plasmid vaccines were delivered to mice by biolistic (gene gun) intradermal inoculation. Following the initial immunization and two consecutive boosts, each at 2-week intervals, protection was demonstrated in a mouse model of chronic pulmonary infection by P. aeruginosa. Eight days postchallenge, both lungs were removed and examined. A significant reduction in the presence of severe macroscopic lesions, as well as in the number of bacteria present in the lungs, was seen (Price et al., 2001).
Our data showed that mice immunized with OprF/OprI or OprF/OprI and flagellin B are significantly protected from infection caused by mucoid and nonmucoid strains of P. aeruginosa.(Hassan et al., 2018).
- Related Vaccine(s):
P. aeruginosa DNA Vaccine encoding OprF
,
P. aeruginosa DNA vaccine pGACAG-OprF/OprI
,
P. aeruginosa DNA vaccine pGACAG-OprF/OprI + pGACAG-PcrV + pGACAG-PilA
|
8. oprF_3 |
-
Gene Name :
oprF_3
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa
-
NCBI Protein GI :
CRR17034
-
Taxonomy ID :
287
-
Protein Name :
Outer membrane porin F precursor
-
Protein pI :
4.63
-
Protein Weight :
37391.91
-
Protein Length :
440
-
Protein Sequence : Show Sequence
>CRR17034.1 Outer membrane porin F precursor [Pseudomonas aeruginosa]
MNKKILTAALLGGLAFAQAASAQEFDDRWYLTGSAGFNFQDSDRLTNDAPFVTLGLGKFISPNWSLDGEL
NYQNPNFDSNQDLNWSQYGVSLDLRRHFIKEGRGWNPYIVGGLGYQKSEEEYAALDNNGPRQRKDGNVAA
KLGVGLQTTFEKRVAVRAEVAYRADFDDQSIAARDESWFGDVLASVGVVIPLGPAPVAAAPAPAPVAPSC
ADLDDDGDGVNNCDDKCPNSQPGQTIGPDGCPVPVSIDLKGVNFDFDKSNLRPDAVAILSEATEILKRYP
DLRVEVAGHTDSKGTDAYNQKLSERRATAVYNYLTKNGVDAGRLVGPIGYGESRPIAPNTNPDGSDNPEG
RAKNRRTELNVQN
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Ahmadi et al., 2012)
|
9. OprI |
-
Gene Name :
OprI
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa PAO1
-
VO ID :
VO_0012362
-
NCBI Gene ID :
879851
-
NCBI Protein GI :
15598049
-
Locus Tag :
PA2853
-
Genbank Accession :
AE004091
-
Protein Accession :
NP_251543
-
Taxonomy ID :
208964
-
Gene Starting Position :
3206914
-
Gene Ending Position :
3207165
-
Gene Strand (Orientation) :
+
-
Protein Name :
outer membrane lipoprotein OprI
-
Protein pI :
8.51
-
Protein Weight :
8635.98
-
Protein Length :
83
-
DNA Sequence : Show Sequence
>NC_002516.2:3206914-3207165 Pseudomonas aeruginosa PAO1 chromosome, complete genome
GATGAACAACGTTCTGAAATTCTCTGCTCTGGCTCTGGCTGCTGTTCTGGCCACCGGTTGCAGCAGCCAC
TCCAAAGAAACCGAAGCTCGTCTGACCGCTACCGAAGACGCAGCTGCTCGTGCTCAGGCTCGCGCTGACG
AAGCCTATCGCAAGGCTGACGAAGCTCTGGGCGCTGCTCAGAAAGCTCAGCAGACTGCTGACGAGGCTAA
CGAGCGTGCCCTGCGCATGCTGGAAAAAGCCAGCCGCAAGTA
-
Protein Sequence : Show Sequence
>NP_251543.1 outer membrane lipoprotein OprI [Pseudomonas aeruginosa PAO1]
MNNVLKFSALALAAVLATGCSSHSKETEARLTATEDAAARAQARADEAYRKADEALGAAQKAQQTADEAN
ERALRMLEKASRK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Recombinant OprI protein was used to immunize mice, and was found to be protective against a challenge with a four- to fivefold 50% lethal dose of P. aeruginosa (Finke et al., 1990).
Our data showed that mice immunized with OprF/OprI are significantly protected from infection caused by mucoid and nonmucoid strains of P. aeruginosa.(Hassan et al., 2018)
- Related Vaccine(s):
P. aeruginosa DNA vaccine pGACAG-OprF/OprI
,
P. aeruginosa DNA vaccine pGACAG-OprF/OprI + pGACAG-PcrV + pGACAG-PilA
,
P. aeruginosa OprI Protein Vaccine
|
10. oprL |
-
Gene Name :
oprL
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa
-
NCBI Protein GI :
ABX84373
-
Other Database IDs :
CDD:225439
CDD:274307 CDD:143586
-
Taxonomy ID :
287
-
Protein Name :
OprL
-
Protein pI :
6.27
-
Protein Weight :
17004.392
-
Protein Length :
214
-
Protein Note :
Outer membrane protein OmpA and related peptidoglycan-associated (lipo)proteins [Cell wall/membrane/envelope biogenesis]; COG2885
-
Protein Sequence : Show Sequence
>ABX84373.1 OprL [Pseudomonas aeruginosa]
MEMLKFGKFAALALAMAVAVGCSSKGGDASGEGANGGVDPNAGYGANSGAVDGSLSDEAALRAITTFYFE
YDSSDLKPEAMRALDVHAKDLKGSGQRVVLEGHTDERGTREYNMALGERRAKAVQRYLVLQGVSPATLEL
VSYGKERPVATGHDEQSWAQNRRVELKK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Soldatenkova et al., 2013)
|
11. PcrV |
-
Gene Name :
PcrV
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa PAO1
-
VO ID :
VO_0012361
-
NCBI Gene ID :
882997
-
NCBI Protein GI :
15596903
-
Locus Tag :
PA1706
-
Genbank Accession :
AE004091
-
Protein Accession :
NP_250397
-
Taxonomy ID :
208964
-
Gene Starting Position :
1852287
-
Gene Ending Position :
1853171
-
Gene Strand (Orientation) :
+
-
Protein Name :
type III secretion protein PcrV
-
Protein pI :
4.77
-
Protein Weight :
30496.99
-
Protein Length :
294
-
DNA Sequence : Show Sequence
>NC_002516.2:1852287-1853171 Pseudomonas aeruginosa PAO1 chromosome, complete genome
GATGGAAGTCAGAAACCTTAATGCCGCTCGCGAGCTGTTCCTGGACGAGCTCCTGGCCGCGTCGGCGGCG
CCTGCCAGTGCCGAGCAGGAGGAACTGCTGGCCCTGTTGCGCAGCGAGCGGATCGTGCTGGCCCACGCCG
GCCAGCCGCTGAGCGAGGCGCAAGTGCTCAAGGCGCTCGCCTGGTTGCTCGCGGCCAATCCGTCCGCGCC
TCCGGGGCAGGGCCTCGAGGTACTCCGCGAAGTCCTGCAGGCACGTCGGCAGCCCGGTGCGCAGTGGGAT
CTGCGCGAGTTCCTGGTGTCGGCCTATTTCAGCCTGCACGGGCGTCTCGACGAGGATGTCATCGGTGTCT
ACAAGGATGTCCTGCAGACCCAGGACGGCAAGCGCAAGGCGCTGCTCGACGAGCTCAAGGCGCTGACCGC
GGAGTTGAAGGTCTACAGCGTGATCCAGTCGCAGATCAACGCCGCGCTGTCGGCCAAGCAGGGCATCAGG
ATCGACGCTGGCGGTATCGATCTGGTCGACCCCACGCTATATGGCTATGCCGTCGGCGATCCCAGGTGGA
AGGACAGCCCCGAGTATGCGCTGCTGAGCAATCTGGATACCTTCAGCGGCAAGCTGTCGATCAAGGATTT
TCTCAGCGGCTCGCCGAAGCAGAGCGGGGAACTCAAGGGCCTCAGCGATGAGTACCCCTTCGAGAAGGAC
AACAACCCGGTCGGCAATTTCGCCACCACGGTGAGCGACCGCTCGCGTCCGCTGAACGACAAGGTCAACG
AGAAGACCACCCTGCTCAACGACACCAGCTCCCGCTACAACTCGGCGGTCGAGGCGCTCAACCGCTTCAT
CCAGAAATACGACAGCGTCCTGCGCGACATTCTCAGCGCGATCTA
-
Protein Sequence : Show Sequence
>NP_250397.1 type III secretion protein PcrV [Pseudomonas aeruginosa PAO1]
MEVRNLNAARELFLDELLAASAAPASAEQEELLALLRSERIVLAHAGQPLSEAQVLKALAWLLAANPSAP
PGQGLEVLREVLQARRQPGAQWDLREFLVSAYFSLHGRLDEDVIGVYKDVLQTQDGKRKALLDELKALTA
ELKVYSVIQSQINAALSAKQGIRIDAGGIDLVDPTLYGYAVGDPRWKDSPEYALLSNLDTFSGKLSIKDF
LSGSPKQSGELKGLSDEYPFEKDNNPVGNFATTVSDRSRPLNDKVNEKTTLLNDTSSRYNSAVEALNRFI
QKYDSVLRDILSAI
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Burned Pseudomonas aeruginosa-infected mice immunized against PcrV, a type III virulence system translocating protein, showed significantly enhanced survival compared to controls (Holder et al., 2001).
The results clearly show that, with PcrV as the vaccine antigen, all three adjuvants tested, FA, alum and CpG ODN, were effective in inducing protective immunity against infection with P. aeruginosa. (Hamaoka et al., 2017)
- Related Vaccine(s):
P. aeruginosa DNA vaccine pGACAG-OprF/OprI + pGACAG-PcrV + pGACAG-PilA
,
P. aeruginosa DNA vaccine pGACAG-PcrV
,
P. aeruginosa PcrV Protein Vaccine
|
12. pilA |
-
Gene Name :
pilA
-
Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa PAO1
-
NCBI Protein GI :
15599721
-
Other Database IDs :
CDD:227304
-
Taxonomy ID :
208964
-
Gene Strand (Orientation) :
?
-
Protein Name :
type 4 fimbrial protein PilA
-
Protein pI :
6.59
-
Protein Weight :
15164.05
-
Protein Length :
225
-
Protein Note :
Tfp pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures]; COG4969
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Protein Sequence : Show Sequence
>NP_253215.1 type 4 fimbrial protein PilA [Pseudomonas aeruginosa PAO1]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGT
TASTATETYVGVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDP
MFTPKGCDN
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Molecule Role :
Protective antigen
- Related Vaccine(s):
P. aeruginosa DNA vaccine pGACAG-OprF/OprI + pGACAG-PcrV + pGACAG-PilA
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13. pilA |
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Gene Name :
pilA
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Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa
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NCBI Protein GI :
AAK68046
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Other Database IDs :
CDD:227304
CDD:278540
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Taxonomy ID :
287
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Protein Name :
PilA
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Protein pI :
9.39
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Protein Weight :
15824.84
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Protein Length :
200
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Protein Note :
Tfp pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures]; COG4969
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Protein Sequence : Show Sequence
>AAK68046.1 PilA [Pseudomonas aeruginosa]
MKAQKGFYLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPK
NDEYDLGFTSSTLLTGSGKGQIKIDKADTATPEISGTLGGSSGKGIAGAVITVKRDDKGVWTCGITGSPT
NWKANYAPANCPKS
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Molecule Role :
Protective antigen
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Molecule Role Annotation :
(Korpi et al., 2015)
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14. pilA |
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Gene Name :
pilA
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Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa
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NCBI Protein GI :
AAC60462
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Other Database IDs :
CDD:227304
CDD:290278 CDD:278540
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Taxonomy ID :
287
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Protein Name :
pilin
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Protein pI :
8.02
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Protein Weight :
14904.71
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Protein Length :
197
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Protein Note :
Tfp pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures]; COG4969
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Protein Sequence : Show Sequence
>AAC60462.1 pilin [Pseudomonas aeruginosa]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGENIKIGS
QASTADTTYVGVDATANKLGTIAVTIGNNGAGTVTFTFAAGNSSPKNSGAIITLTRTDAGSWACTSTQDE
MFIPKGCKAP
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Molecule Role :
Protective antigen
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Molecule Role Annotation :
(Banadkoki et al., 2016)
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15. popB |
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Gene Name :
popB
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Sequence Strain (Species/Organism) :
Pseudomonas aeruginosa
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NCBI Protein GI :
AAC45937
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Other Database IDs :
CDD:227900
CDD:279450
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Taxonomy ID :
287
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Protein Name :
PopB
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Protein pI :
5.88
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Protein Weight :
37740.4
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Protein Length :
441
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Protein Note :
Uncharacterized protein [Function unknown]
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Protein Sequence : Show Sequence
>AAC45937.1 PopB [Pseudomonas aeruginosa]
MNPITLERAGLPYGVADAGDIPALGRPVARDVESLRVERLAAPAAASASGTGVALTPPSAASQQRLEVAN
RAEIASLVQAVGEDVGLARQVVLAGASTLLSAGLMSPQAFEIELAKITGEVENQQKKLKLTEIEQARKQN
LQKMEDNQQKIRESEEAAKEAQKSGLAAKIFGWISAIASIIVGAIMVATGVGAAAGALMIAGGVMGVVSQ
SVQQAAADGLISKEVMEKLGPALMGIEIAVALLAAVVSFGGSAVGGLARLGAKIGGKAAEMTASLASKVA
DLGGKFGSLAGQSLSHSLKLGVQVSDLTLDVANGAAQATHSGFQAKAANRQADVQESRADLTTLQGVIER
LKEELSRMCMLEAFQEIMERIFAMLQAKGETLHNLSSRPAAI
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Molecule Role :
Protective antigen
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Molecule Role Annotation :
(Wu et al., 2012)
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III. Vaccine Information |
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1. Mink Distemper-Enteritis Modified Live & Killed Virus Vaccine-Clostridium Botulinum Type C-Pseudomonas Aeruginosa Bacterin-Toxoid (USDA: 4949.20) |
a. Manufacturer: |
Intervet Inc. |
b. Vaccine Ontology ID: |
VO_0001834 |
c. Type: |
Live, attenuated vaccine; Inactivated or "killed" vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Carnivores |
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2. Mink Distemper-Enteritis Modified Live & Killed Virus Vaccine-Clostridium Botulinum Type C-Pseudomonas Aeruginosa Bacterin-Toxoid (USDA: 4949.31) |
a. Manufacturer: |
United Vaccines, Inc. |
b. Vaccine Ontology ID: |
VO_0001835 |
c. Type: |
Live, attenuated vaccine; Inactivated or "killed" vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Carnivores |
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3. Mink Enteritis Killed Virus Vaccine-Clostridium Botulinum Type C-Pseudomonas Aeruginosa Bacterin-Toxoid (USDA: 49A5.20) |
a. Manufacturer: |
Intervet Inc. |
b. Vaccine Ontology ID: |
VO_0001839 |
c. Type: |
Inactivated or "killed" vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Carnivores |
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4. Mink Enteritis Killed Virus Vaccine-Clostridium Botulinum Type C-Pseudomonas Aeruginosa Bacterin-Toxoid (USDA: 49A5.21) |
a. Manufacturer: |
United Vaccines, Inc. |
b. Vaccine Ontology ID: |
VO_0001840 |
c. Type: |
Inactivated or "killed" vaccine |
d. Status: |
Licensed |
e. Location Licensed: |
USA |
f. Host Species for Licensed Use: |
Carnivores |
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5. P. aeruginosa DNA Vaccine encoding OprF |
a. Vaccine Ontology ID: |
VO_0004189 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Antigen |
The pVR1020/oprF plasmid. |
e. Gene Engineering of
OprF |
- Type:
DNA vaccine construction
- Description:
The 977-bp oprF gene was cloned from P. aeruginosa PAO1 genomic DNA into the eukaryotic expression plasmid pVR1020 (Price et al., 2001).
- Detailed Gene Information: Click here.
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f. Vector: |
pVR1020 |
g. Immunization Route |
abdomen gene gun |
h.
Mouse Response |
- Host Strain:
Pathogen Free ICR
- Vaccination Protocol:
At 14-day intervals, groups of 30 mice were inoculated a total of three times in the abdomen via biolistic particle injection (Helios Gene Gun kit; Bio-Rad, Richmond, Calif.) on days 0, 14, and 28 with 2 μg of either pVR1020 (control) or pVR1020/oprF, which was used to coat 1-μm-diameter gold beads (Price et al., 2001).
- Challenge Protocol:
Two weeks after the final immunization, the mice were challenged with agar beads containing the P. aeruginosa FD immunotype 4 strain. The mice were first anesthetized with an intraperitoneal injection of sodium pentobarbital and then inoculated via a tracheal incision with 50 μl of an agar bead slurry encasing approximately 7 × 10^2 CFU of P. aeruginosa (Price et al., 2001).
- Efficacy:
A significant reduction in the presence of severe macroscopic lesions, as well as in the number of bacteria present in the lungs, was seen in immunized mice (Price et al., 2001).
- Description:
The immunoprotective potential of the pVR1020/oprF vaccine was tested in a mouse model of chronic pulmonary infection (Price et al., 2001).
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6. P. aeruginosa DNA vaccine pGACAG-OprF/OprI |
a. Vaccine Ontology ID: |
VO_0004570 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Mouse |
e. Gene Engineering of
OprF |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
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f. Gene Engineering of
OprI |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
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g. Vector: |
pGACAG (Saha et al., 2006) |
h. Immunization Route |
intramuscular electroporation |
i.
Mouse Response |
- Vaccine Immune Response Type:
VO_0003057
- Efficacy:
Monovalent DNA vaccination targeting OprF/OprI could protect 80% of mice 4 days p.i. On the other hand, 70% of mice immunized with the control plasmid, PilA vaccine, or multivalent vaccine via GG were dead within 5 days p.i. (p < 0.01) (Saha et al., 2006).
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7. P. aeruginosa DNA vaccine pGACAG-OprF/OprI + pGACAG-PcrV + pGACAG-PilA |
a. Vaccine Ontology ID: |
VO_0004572 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Mouse |
e. Gene Engineering of
OprF |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
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f. Gene Engineering of
OprI |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
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g. Gene Engineering of
PcrV |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
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h. Gene Engineering of
pilA |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
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i. Vector: |
pGACAG (Saha et al., 2006) |
j. Immunization Route |
intramuscular electroporation |
k.
Mouse Response |
- Vaccine Immune Response Type:
VO_0003057
- Efficacy:
All mice vaccinated with multivalent DNA via imEPT survived for more than 10 days p.i. with PAK. On the other hand, 70% of mice immunized with the control plasmid, PilA vaccine, or multivalent vaccine via GG were dead within 5 days p.i. (p < 0.01) (Saha et al., 2006).
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8. P. aeruginosa DNA vaccine pGACAG-PcrV |
a. Vaccine Ontology ID: |
VO_0004571 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Mouse |
e. Gene Engineering of
PcrV |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
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f. Vector: |
pGACAG (Saha et al., 2006) |
g. Immunization Route |
intramuscular electroporation |
h.
Mouse Response |
- Vaccine Immune Response Type:
VO_0003057
- Efficacy:
Monovalent DNA vaccination targeting PcrV could protect 80% of mice 4 days p.i. On the other hand, 70% of mice immunized with the control plasmid, PilA vaccine, or multivalent vaccine via GG were dead within 5 days p.i. (p < 0.01) (Saha et al., 2006).
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9. P. aeruginosa OprI Protein Vaccine |
a. Vaccine Ontology ID: |
VO_0004190 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Antigen |
Recombinant OprI protein |
e. Gene Engineering of
OprI |
- Type:
Recombinant protein preparation
- Description:
- Detailed Gene Information: Click here.
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f. Adjuvant: |
- VO ID:
VO_0000127
- Description:
100 μl of 1.5% Al(OH)3 (Finke et al., 1990).
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g. Immunization Route |
Intraperitoneal injection (i.p.) |
h.
Mouse Response |
- Host Strain:
BALB/c
- Vaccination Protocol:
Female BALB/c mice, 12 to 16 weeks old, received 100 μl (27 ,ug) of OprI suspended in 100 μl of 1.5% Al(OH)3 intraperitoneally on days 0, 14, 35, and 63. Controls received Al(OH)3 only (Finke et al., 1990).
- Challenge Protocol:
mice were challenged intraperitoneally 10 days after the last immunization with 200 μl of a P. aeruginosa serogroup 6 suspension. Mice vaccinated with recombinant OprI received 6 x 10^6 to 1 x 10^8 living organisms each. (Finke et al., 1990).
- Efficacy:
Recombinant OprI protein was used to immunize mice, and was found to be protective against a challenge with a four- to fivefold 50% lethal dose of P. aeruginosa (Finke et al., 1990).
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10. P. aeruginosa PcrV Protein Vaccine |
a. Vaccine Ontology ID: |
VO_0004191 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Antigen |
PcrV |
e. Gene Engineering of
PcrV |
- Type:
Recombinant protein preparation
- Description:
PcrV was produced as a lipopolysaccharide-free histidine-tagged infusion protein in pET16b and was purified by nickel chromatography (Holder et al., 2001).
- Detailed Gene Information: Click here.
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f. Adjuvant: |
- VO ID:
VO_0000142
- Description:
0.1 ml of incomplete Freund's adjuvant (Holder et al., 2001).
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g. Immunization Route |
Intramuscular injection (i.m.) |
h.
Mouse Response |
- Host Strain:
CF-1
- Vaccination Protocol:
On day 0, groups of 10 female CF-1 mice weighing 22 to 25 g were immunized intramuscularly in the hind leg (10 μg of immunogen in 0.1 ml of incomplete Freund's adjuvant), followed by a booster dose (10 μg in saline) without adjuvant on day 14 (Holder et al., 2001).
- Challenge Protocol:
Using the burned mouse model, mice were challenged by one of three strains of P. aeruginosa (Holder et al., 2001).
- Efficacy:
Burned Pseudomonas aeruginosa-infected mice immunized against PcrV, a type III virulence system translocating protein, showed significantly enhanced survival compared to controls (Holder et al., 2001).
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11. SL3261-P. aeruginosa serogroup O11 O antigen |
a. Vaccine Ontology ID: |
VO_0004674 |
b. Type: |
Recombinant vector vaccine |
c. Status: |
Research |
d. Host Species for Licensed Use: |
Baboon |
e. Preparation |
Attenuated Salmonella enterica serovar Typhimurium SL3261 expressing P. aeruginosa serogroup O11 O antigen (DiGiandomenico et al., 2004). |
f. Immunization Route |
Intramuscular injection (i.m.) |
g.
Mouse Response |
- Vaccination Protocol:
For oral vaccination, mice were fed 100 μl of either PBS or the Salmonella vector and vaccine strains (1 × 10^9 to 5 × 10^9 CFU) by intragastric gavage. Oral inoculation was repeated once per week for a total of 4 weeks. For i.p. vaccination, mice were inoculated with a single dose of either PBS or each Salmonella strain (10^6 CFU) (DiGiandomenico et al., 2004).
- Vaccine Immune Response Type:
VO_0003057
- Challenge Protocol:
Mice were challenged with P. aeruginosa strains 9882-80 (serogroup O11) and 6294 (serogroup O6) (DiGiandomenico et al., 2004).
- Efficacy:
Orally vaccinated mice with an O11 strain (9882-80) at 6 and 12 times the 50% lethal dose showed increased survival in mice that received the vaccine compared to phosphate-buffered saline (PBS)- and vector-treated controls; no difference in survival was seen with a heterologous strain, 6294 (serogroup O6). In addition, significant protection against 9882-80 was not observed in i.p. vaccinated animals (DiGiandomenico et al., 2004).
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IV. References |
1. Ahmadi et al., 2012: Ahmadi H, Tabaraie B, Maleknia S, Pormirzagholi F, Nejati M, Hedayati MH. Immunological evaluation of OMP-F of native Iranian Pseudomonas aeruginosa as a protective vaccine. Journal of infection in developing countries. 2012; 6(10); 721-726. [PubMed: 23103894].
2. Banadkoki et al., 2016: Banadkoki AZ, Keshavarzmehr M, Afshar Z, Aleyasin N, Fatemi MJ, Behrouz B, Hashemi FB. Protective effect of pilin protein with alum+naloxone adjuvant against acute pulmonary Pseudomonas aeruginosa infection. Biologicals : journal of the International Association of Biological Standardization. 2016; 44(5); 367-373. [PubMed: 27427517].
3. Behrouz et al., 2016: Behrouz B, Mahdavi M, Amirmozafari N, Fatemi MJ, Irajian G, Bahroudi M, Hashemi FB. Immunogenicity of Pseudomonas aeruginosa recombinant b-type fagellin as a vaccine candidate: Protective efficacy in a murine burn wound sepsis model. Burns : journal of the International Society for Burn Injuries. 2016; ; . [PubMed: 27156804].
4. Campodónico et al., 2010: Campodónico VL, Llosa NJ, Grout M, Döring G, Maira-Litrán T, Pier GB. Evaluation of flagella and flagellin of Pseudomonas aeruginosa as vaccines. Infection and immunity. 2010; 78(2); 746-755. [PubMed: 19995892].
5. Finke et al., 1990: Finke M, DuchĂŞne M, Eckhardt A, Domdey H, von Specht BU. Protection against experimental Pseudomonas aeruginosa infection by recombinant P. aeruginosa lipoprotein I expressed in Escherichia coli. Infection and immunity. 1990; 58(7); 2241-2244. [PubMed: 2114360].
6. Hamaoka et al., 2017: Hamaoka S, Naito Y, Katoh H, Shimizu M, Kinoshita M, Akiyama K, Kainuma A, Moriyama K, Ishii KJ, Sawa T. Efficacy comparison of adjuvants in PcrV vaccine against Pseudomonas aeruginosa pneumonia. Microbiology and immunology. 2017; 61(2); 64-74. [PubMed: 28370521].
7. Hassan et al., 2018: Hassan R, El-Naggar W, Abd El-Aziz AM, Shaaban M, Kenawy HI, Ali YM. Immunization with outer membrane proteins (OprF and OprI) and flagellin B protects mice from pulmonary infection with mucoid and nonmucoid Pseudomonas aeruginosa. Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi. 2018; 51(3); 312-320. [PubMed: 28291719].
8. Holder et al., 2001: Holder IA, Neely AN, Frank DW. PcrV immunization enhances survival of burned Pseudomonas aeruginosa-infected mice. Infection and immunity. 2001; 69(9); 5908-5910. [PubMed: 11500471].
9. Korpi et al., 2015: Korpi F, Irajian G, Mahadavi M, Motamedifar M, Mousavi M, Laghaei P, Raei N, Behrouz B. Active Immunization with Recombinant PilA protein Protects Against Pseudomonas aeruginosa Infection in a Mouse Burn Wound Model. Journal of microbiology and biotechnology. 2015; ; . [PubMed: 26387817].
10. Laghaei et al., 2016: Laghaei P, Hashemi FB, Irajian G, Korpi F, Amirmozafari N, Behrouz B. Immunogenicity and protective efficacy of Pseudomonas aeruginosa type a and b flagellin vaccines in a burned mouse model. Molecular immunology. 2016; 74; 71-81. [PubMed: 27152476].
11. Price et al., 2001: Price BM, Galloway DR, Baker NR, Gilleland LB, Staczek J, Gilleland HE Jr. Protection against Pseudomonas aeruginosa chronic lung infection in mice by genetic immunization against outer membrane protein F (OprF) of P. aeruginosa. Infection and immunity. 2001; 69(5); 3510-3515. [PubMed: 11292786].
12. Rehm et al., 1994: Rehm BH, Grabert E, Hein J, Winkler UK. Antibody response of rabbits and cystic fibrosis patients to an alginate-specific outer membrane protein of a mucoid strain of Pseudomonas aeruginosa. Microbial pathogenesis. 1994; 16(1); 43-51. [PubMed: 8057826].
13. Saha et al., 2006: Saha S, Takeshita F, Sasaki S, Matsuda T, Tanaka T, Tozuka M, Takase K, Matsumoto T, Okuda K, Ishii N, Yamaguchi K, Klinman DM, Xin KQ, Okuda K. Multivalent DNA vaccine protects mice against pulmonary infection caused by Pseudomonas aeruginosa. Vaccine. 2006; 24(37-39); 6240-6249. [PubMed: 16806598].
14. Salyers and Whitt., 2002: Abigail A. Salyers, Dixie D. Whitt. Pseudomonas aeruginosa and Related Species, a Lesson in Versatility. 247-62. Bacterial Pathogenesis: A Molecular Approach. 2002. ASM Press, Washington D.C. USA.
15. Soldatenkova et al., 2013: Soldatenkova AV, Ge?derova LA, Akhmatova NK, Mikha?lova NA. [Pseudomonas aeruginosa recombinant proteins: effect on mice cytokine profile]. Zhurnal mikrobiologii, epidemiologii, i immunobiologii. 2013; (6); 80-87. [PubMed: 24605680].
16. Textbook of Bacteriology: Pseudomonas [http://www.textbookofbacteriology.net/pseudomonas.html]
17. Thomas et al., 2009: Thomas LD, Cripps AW, Kyd JM. Immune response mechanisms against Pseudomonas aeruginosa associated with mucosal immunization with protein antigens in a rat model of acute lung infection. Vaccine. 2009; 27(25-26); 3324-3330. [PubMed: 19200832].
18. Wu et al., 2012: Wu W, Huang J, Duan B, Traficante DC, Hong H, Risech M, Lory S, Priebe GP. Th17-stimulating protein vaccines confer protection against Pseudomonas aeruginosa pneumonia. American journal of respiratory and critical care medicine. 2012; 186(5); 420-427. [PubMed: 22723292].
19. Yang et al., 2017: Yang F, Gu J, Yang L, Gao C, Jing H, Wang Y, Zeng H, Zou Q, Lv F, Zhang J. Protective Efficacy of the Trivalent Pseudomonas aeruginosa Vaccine Candidate PcrV-OprI-Hcp1 in Murine Pneumonia and Burn Models. Scientific reports. 2017; 7(1); 3957. [PubMed: 28638106].
20. Zaidi et al., 2006: Zaidi TS, Priebe GP, Pier GB. A live-attenuated Pseudomonas aeruginosa vaccine elicits outer membrane protein-specific active and passive protection against corneal infection. Infection and immunity. 2006; 74(2); 975-983. [PubMed: 16428743].
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