|
Burkholderia pseudomallei |
Table of Contents |
- General Information
- NCBI Taxonomy ID
- Disease
- Introduction
- Microbial Pathogenesis
- Host Ranges and Animal Models
- Vaccine Related Pathogen Genes
- bipB
(Protective antigen)
- BipD
(Protective antigen)
- bopE
(Protective antigen)
- flgK
(Protective antigen)
- fliC
(Protective antigen)
- groEL
(Protective antigen)
- LolC
(Protective antigen)
- LolC/E
(Protective antigen)
- ompW
(Protective antigen)
- oppA
(Protective antigen)
- PotF
(Protective antigen)
- PotF
(Protective antigen)
- ttsS
(Protective antigen)
- aroC
(Virmugen)
- asd
(Virmugen)
- purM
(Virmugen)
- purN
(Virmugen)
- Vaccine Related Host Genes
- Ifng (Interferon gamma)
- Vaccine Information
- B. pseudomallei DNA vaccine encoding Flagellin Protein
- B. pseudomallei Subunit LolC Protein Vaccine
- B. pseudomallei Subunit PotF Protein Vaccine
- Burkholderia pseudomallei aroC deletion mutant vaccine
- Burkholderia pseudomallei asd mutant vaccine
- Burkholderia pseudomallei purM mutant vaccine
- Burkholderia pseudomallei purN mutant vaccine
- References
|
I. General Information |
1. NCBI Taxonomy ID: |
28450 |
2. Disease: |
Melioidosis |
3. Introduction |
The bacterium Burkholderia pseudomallei is a gram-negative saprophytic bacillus that is found predominantly in Southeast Asia and tropical Australia and is the causative agent of the emerging infection melioidosis. Infection of humans with B. pseudomallei may occur by various routes, including via wounds and existing skin lesions, aspiration of contaminated water during near-drowning, and inhalation of organisms. The clinical manifestations of melioidosis are not uniform, and patients can present with pneumonia, skin abscesses, soft tissue abscesses, or osteomyelitis/septic arthritis. Pneumonic melioidosis is presented in over 50% of acute cases of the disease, and disseminated pneumonia is associated with high mortality. Chronic melioidosis usually occurs following acute melioidosis, and relapsing melioidosis can result from the reactivation of a latent B. pseudomallei infection, often due to the withdrawal of antibiotics or the failure to complete prescribed courses of antibiotics (Harland et al., 2007). |
4. Microbial Pathogenesis |
B. pseudomallei is an "accidental pathogen". It is an environmental organism that has no requirement to pass through an animal host in order to replicate. From the point of view of the bacterium, human infection is an evolutionary "dead end". B. pseudomallei is able to invade cells, polymerise actin and to spread from cell to cell, causing cell fusion and the formation of multinucleate giant cells (Wiki: B. pseudomallei). |
5. Host Ranges and Animal Models |
B. pseudomallei infects humans and animals and causes the disease melioidosis. It is also capable of infecting plants (Wiki: B. pseudomallei). |
II. Vaccine Related Pathogen Genes |
1. aroC |
-
Gene Name :
aroC
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei 1106a
-
NCBI Gene ID :
4899749
-
NCBI Protein GI :
126453136
-
Locus Tag :
BURPS1106A_1713
-
Genbank Accession :
CP000572
-
Protein Accession :
YP_001065984
-
Taxonomy ID :
357348
-
Chromosome No :
I
-
Gene Starting Position :
1672513
-
Gene Ending Position :
1673622
-
Gene Strand (Orientation) :
+
-
Protein Name :
chorismate synthase
-
Protein pI :
6.35
-
Protein Weight :
36184.83
-
Protein Length :
369
-
Protein Note :
catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis
-
DNA Sequence : Show Sequence
>gi|126451443:1672513-1673622 Burkholderia pseudomallei 1106a chromosome I, complete genome
TATGTCCGGCAACACCCTCGGCACGCTTTTCACTGTCACGACCTTCGGCGAATCGCACGGCCCCGCGATC
GGCTGCGTGATCGACGGCTGCCCGCCGGGCATGGCGCTCACGGAAGCCGACGTCCAGCTCGAGCTCGACC
GCCGCAAGCCCGGCACGTCGCGCCACGTCACGCAGCGTCAGGAGCCCGACCAGGTCGAGATCCTGTCCGG
CGTGTTCGAGGGCGTGACGACCGGCGCGCCGATCGCGCTCCTGATCCGCAACACCGACCAGCGCAGCAAG
GACTACGGCAACATCGCCGAGACGTTCCGCCCGGGCCATGCCGATTACACCTACTGGCAAAAGTACGGCG
TGCGCGACTATCGCGGCGGCGGCCGCTCGTCCGCGCGGCTGACGGCGCCCGTCGTCGGCGCCGGCGCGAT
CGCGAAGAAGTGGCTGCGCGAGCGCTTCGGCGTCGAGGTGCGCGGCTACATGAGCGCGCTCGGCGAAATC
GAGATCCCGTTCGTCGACTGGTCGCACGTGCGCGAGAACCCGTTCTTCGCGCCGAACGCCGACATCGTGC
CGCAACTCGAGGGCTACATGGACGCGCTGCGCAAGGACGGCGATTCGATCGGCGCGCGCATCGATGTCGT
CGCGTCGGGCGTGCCGGTCGGCTGGGGCGAGCCGCTGTTCGACCGGCTCGACGCCGACATCGCGCACGCG
ATGATGGGGATCAACGCGGTGAAGGGCGTCGAGATCGGCGCGGGTTTCGCGAGCGTCGCGCAGCGCGGTT
CGGTGCACGGCGACGAGCTGACGCCGGACGGCTTCGTCGGCAATCACGCGGGCGGCGTGCTCGGCGGCAT
CTCGACGGGGCAGGACATCACGGTGTCGATCGCGATCAAGCCGACGTCGAGCATTCGCACGCCGCGCCGC
TCGATCACGCGGGCGGGCGAACCCGCCGTCGTCGAGACGTTCGGCCGCCACGACCCGTGCGTCGGGATTC
GCGCGACGCCGATCGCCGAATCGATGCTCGCGCTCGTGCTGATCGATCACGCGCTGCGGCACCGCGCGCA
GTGCGGCGACGTGTCGAGCGCGACGCCGAGGATCGCCGCGCGCGCGCCGGACGCGCAATG
-
Protein Sequence : Show Sequence
>gi|126453136|ref|YP_001065984.1| chorismate synthase [Burkholderia pseudomallei 1106a]
MSGNTLGTLFTVTTFGESHGPAIGCVIDGCPPGMALTEADVQLELDRRKPGTSRHVTQRQEPDQVEILSG
VFEGVTTGAPIALLIRNTDQRSKDYGNIAETFRPGHADYTYWQKYGVRDYRGGGRSSARLTAPVVGAGAI
AKKWLRERFGVEVRGYMSALGEIEIPFVDWSHVRENPFFAPNADIVPQLEGYMDALRKDGDSIGARIDVV
ASGVPVGWGEPLFDRLDADIAHAMMGINAVKGVEIGAGFASVAQRGSVHGDELTPDGFVGNHAGGVLGGI
STGQDITVSIAIKPTSSIRTPRRSITRAGEPAVVETFGRHDPCVGIRATPIAESMLALVLIDHALRHRAQ
CGDVSSATPRIAARAPDAQ
-
Molecule Role :
Virmugen
-
Molecule Role Annotation :
The aroC deletion in the resultant mutant, designated A2ΔaroC, was confirmed by PCR, Southern hybridization and failure of the mutant to grow in a defined medium without aromatic compounds. The constructed unmarked aroC mutant of B. pseudomallei is attenuated in both BALB/c and C57Bl/6 mice and is able to confer significant protection in C57Bl/6 mice (Srilunchang et al., 2009).
- Related Vaccine(s):
Burkholderia pseudomallei aroC deletion mutant vaccine
|
2. asd |
-
Gene Name :
asd
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei 1026b
-
NCBI Protein GI :
2358232
-
Other Database IDs :
CDD:200773
CDD:162144
-
Taxonomy ID :
884204
-
Chromosome No :
II
-
Gene Starting Position :
2291554
-
Gene Ending Position :
2292675
-
Gene Strand (Orientation) :
-
-
Protein Name :
aspartate beta-semialdehyde dehydrogenase
-
Protein pI :
5.09
-
Protein Weight :
37414.1
-
Protein Length :
90
-
Protein Note :
Rossmann-fold NAD(P)(+)-binding proteins; cl09931
-
DNA Sequence : Show Sequence
>gi|126455463:2291554-2292675 Burkholderia pseudomallei 1106a chromosome chromosome II, complete sequence
TTTACTTGTCGAGCAGAATGCGCAGCATCCGGCGCAGCGGCTCGGCCGCGCCCCACAGCAATTGATCGCC
GACGGTAAATGCCGACAGGTATTCGCCACCCATCGCGAGCTTGCGCAGGCGGCCGACCGGCACCGACAGC
GTGCCCGTCACCTTCGCCGGCGACAGATCGCGCATCGACGCCTCGCGCTCGTTCGGCACGACCTTCACCC
AGTCGTTCGCCGATGCGAGCATGCCGTTGATCTCGTCGAGCGGCACGTCCTTCTTCAGCTTGATCGTGAG
CGCCTGCGAGTGGCAGCGCATCGCGCCGATCCGCACGCACAGGCCGTCGACCGGAATCGAGCCCGGCTCG
CCCATCGCCGGCTTGCCGAGGATCTTGTTGGTTTCCGCGCCGCCCTTCCACTCTTCCTTCGACATGCCGT
TGCCGAGATCCTTGTCGATCCACGGAATCAGCGAGCCGGCAAGCGGCACGCCGAACTGGCTCGTCGGCAT
CGCGTCGCCGTTCATCGCGGCGAGCACGCGGCGGTCGATATCGAGGATCGCCGAGGCCGGATCGGCGAGC
TGCGCCGCCACCGCGCCGTTGAGCGCGCCCATCTGCGCGAGCAGCTCGCGCATGTTCTGCGCGCCCGCGC
CCGACGCGGCCTGGTAGGTCATCGCCGTCATCCAGTCGACGAGGTTCTCGCGGAACAGGCCGCCCAGCGC
CATCAGCATCAGGCTGACCGTGCAGTTGCCGCCGATGAAGTTCTTCGTGCCGTTGACGAGCGCGTCCTTG
ATCACGTTCAGGTTCACGGGATCGAGAATGATCACCGCGTCGTCCTTCATCCGCAGCGACGACGCCGCAT
CGATCCAGTAGCCGTTCCAGCCGGCCGCGCGCAGCTTCGGGAACACGTCGCTCGTGTAATCGCCGCCCTG
GCACGTGATGATCGCGTCGCACTTCTTCAGGTCGTCGATGCTCGTCGCATCCTTGAGCGTGGTCTCGTTT
TTCGCGAACGACGGCGCCTTGCCGCCCGCGTTGCTGGTGCTGAAAAACACCGGCTCGATCAGATCGAAAT
CGCCTTCTTCCTGCATGCGCTGCATCAGCACGCTGCCGACCATGCCGCGCCAACCTACGAGACCTACGTT
CA
-
Protein Sequence : Show Sequence
>gi|2358232|gb|AAB69093.1| aspartate beta-semialdehyde dehydrogenase [Burkholderia pseudomallei 1026b]
CQGGDNASDVFPKPRAAGWNGYWIDAASSLRMKDDAVIILDPVNLNVIKDALVNGTKNFIGGNCTVSLML
MALDGLFRENLVDWMTAMTY
-
Molecule Role :
Virmugen
-
Molecule Role Annotation :
An asd mutant is highly attenuated in mice. This mutant was also able to protect against acute inhalation melioidosis caused by challenge with wild type B. pseudomallei (Norris et al., 2011).
- Related Vaccine(s):
Burkholderia pseudomallei asd mutant vaccine
|
3. bipB |
-
Gene Name :
bipB
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei
-
NCBI Protein GI :
ABO26356
-
Other Database IDs :
CDD:332605
-
Taxonomy ID :
28450
-
Protein Name :
BipB
-
Protein pI :
6.41
-
Protein Weight :
60578.26
-
Protein Length :
675
-
Protein Note :
PHLS = Public Health Laboratory Service, Colindale, United Kingdom
-
Protein Sequence : Show Sequence
>ABO26356.1 BipB [Burkholderia pseudomallei]
MSSGVQGGPAAHANAYQTHPLRDAASALGTLSPQAYVDVVSAAQRNFLERMSQLASEQCDAQPAAHDARL
DDKPALRAPQERDAPPLGASDTGSRASGAAKLTELLGVLMSVISASSLDELKQRSDIWNQMSKAAQDNLS
RLSDAFQRATDEAKAAADAAEQAAAAAKQAGADAKAADAAVDAAQKRYDDAVKQGLPDDRLQSLKAALEQ
ARQQAGDAHGRADALQADATKKLDAASALATQARACEQQVDDAVNQATQQYGASASLRTPQSPRLSGAAE
LTAVLGKLQELISSGNVKELESKQKLFTEMQAKREAELQKKSDEYQAQVKKAEEMQKTMGCIGKIVGWVI
TAVSFAAAAFTGGASLALAAVGLALAVGDEISRATTGVSFMDKLMQPVMDAILKPLMEMISSLITKALVA
CGVDQQKAELAGAILGAVVTGVALVAAAFVGASAVKAVASKVIDAMAGQLTKLMDSAIGKMLVQLIEKFS
EKSGLQALGSRTATAMTRMRRAIGVEAKEDGMLLANRFEKAGTVMNVGNQVSQAAGGIVVGVERAKAMGL
LADVKEAMYDIKLLGDLLKQAVDAFAEHNRVLAQLMQQMSDAGEMQTSTGKLILRNARAV
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Druar et al., 2008)
|
4. BipD |
-
Gene Name :
BipD
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei
-
NCBI Protein GI :
ABL67521
-
Other Database IDs :
CDD:297251
-
Taxonomy ID :
28450
-
Protein Name :
BipD
-
Protein pI :
4.8
-
Protein Weight :
32250.93
-
Protein Length :
361
-
Protein Note :
Invasion plasmid antigen IpaD; cl05827
-
Protein Sequence : Show Sequence
>ABL67521.1 BipD [Burkholderia pseudomallei]
MNMHVDMGRALTVRDWPALEALAKTMPADAGAREMTDDDLRAAGVDRRVPEQKLGAAIDEFASLRLPDRI
DGRFVDGRRANLTVFDDARVAVRGHARAQRNLLERLETELLGGTLDTAGDEGGIQPDPILQGLVDVIGQG
KSDIDAYATIVEGLTKYFQSVADVMSKLQDYISAKDDKNMKIDGGKIKALIQQVIDHLPTMQLPKGADIA
RWRKELGDAVSISDSGVVTINPDKLIKMRDSLPPDGTVWDTARYQAWNTAFSGQKDNIQNDVQTLVEKYS
HQNSNFDNLVKMLSGAISTLTDAAKSYLQI
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Haque et al., 2006)
|
5. bopE |
-
Gene Name :
bopE
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei
-
NCBI Protein GI :
CRY24974
-
Taxonomy ID :
28450
-
Protein Name :
type III secretion target BopE
-
Protein pI :
9.7
-
Protein Weight :
28137.59
-
Protein Length :
337
-
Protein Sequence : Show Sequence
>CRY24974.1 type III secretion target BopE [Burkholderia pseudomallei]
MTYNPRIGGFTHVKQASFDVHVKRGEAQPRTSFAQQIKRIFSKIGETLGQLFRHRAPDSAPGRVRLQGVR
YVGSYRPTGDAKQAIRHFVDEAVKQVAHTRTPEIRQDAEFGRQVYEATLCAIFSEAKDRFCMDPATRAGN
VRPAFIEALGDAARATGLPGADKQGVFTPSGAGTNPLYTEIRLRADTLMGAELAARPEYRELQPYARQQA
IDLVANALPAERSNTLVEFRQTVQTLEATYRRAAQDASRDEKGATNAADGA
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Haque et al., 2006)
|
6. flgK |
-
Gene Name :
flgK
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei
-
NCBI Protein GI :
AIV80017
-
Other Database IDs :
CDD:235895
-
Taxonomy ID :
28450
-
Protein Name :
flagellar hook-associated protein FlgK
-
Protein pI :
4.57
-
Protein Weight :
62777.52
-
Protein Length :
757
-
Protein Note :
flagellar hook-associated protein FlgK; Validated; PRK06945
-
Protein Sequence : Show Sequence
>AIV80017.1 flagellar hook-associated protein FlgK [Burkholderia pseudomallei]
MSNTLMNLGVSGLNAALWGLTTTGQNISNAATPGYSVERPVYAEASGQYTSSGYLPQGVSTVTVERQYNQ
YLSNQLNAAQTQGSSLSTYYTLVAQLNNYVGSPTAGIATAITNYFTGLQTVANNAADPSARQTAMSNAQT
LASQLVAAGQQYSQLRQSVNSQLTDTVTQINSYTSQIAQLNEQIASASSQGQPPNQLLDQRDLAVSKLSQ
LAGVQVVQSNGNYSVFLSGGQPLVVGNASYQLATVASPSDPSELTIVSKGVAGSAQPGPTQYLPDVSLTG
GALGGLLAFRSQTLDPAQAQLGALAVSFASQVNAQNALGVDMSGNPGGSLFAVGAPAVYANQNNTGSATL
SVSFVDGTQPTTSDYALSYDGAKYTLTDRATGSVVGTATPSSTPPTMTIGGLKLSLSSTPNAGDSFTVLP
TRGALDGFSLATANGSAIAAASPVLAAGVATNSGTGVISQGSVSAGYQLPSGTTTLAYNAASKTLSGFPV
GTTVTIAGTPPTSINITSATTPVPYDPSKGASMTISSTTQPAPSGVMNGVSVSLSGTPADGDQFTIGANK
GTNDGRNALALSQLVNSKTMNNGTTTLTGAYAGYVNAIGNAASQLKASSAAQTALVGQITQAQQSVSGVN
QNEEAANLMQYQQLYQANAKVIQTANSVFQTVLGLFN
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Gourlay et al., 2015)
|
7. fliC |
-
Gene Name :
fliC
-
VO ID :
VO_0011303
-
NCBI Protein GI :
1929918
-
Other Database IDs :
CDD:236344
-
Taxonomy ID :
884204
-
Gene Strand (Orientation) :
?
-
Protein Name :
flagellin
-
Protein pI :
4.84
-
Protein Weight :
37016.41
-
Protein Length :
451
-
Protein Note :
flagellin; Reviewed
-
Protein Sequence : Show Sequence
>AAB51476.1 flagellin [Burkholderia pseudomallei 1026b]
MLGINSNINSLVAQQNLNGSQGALSQAITRLSSGKRINSAADDAAGLAIATRMQTQINGLNQGVSNANDG
VSILQTASSGLTSLTNSLQRIRQLAVQASNGPLSASDASALQQEVAQQISEVNRIASQTNYNGKNILDGS
AGTLSFQVGANVGQTVSVDLTQSMSAAKIGGGMVQTGQTLGTIKVAIDSSGAAWSSGSTGQETTQINVVS
DGKGGFTFTDQNNQALSSTAVTAVFGSSTAGTGTAASPSFQTLALSTSATSALSATDQANATAMVAQINA
VNKPQTVSNLDISTQTGAYQAMVSIDNALATVNNLQATLGAAQNRFTAIATTQQAGSNNLAQAQSQIQSA
DFAQETANLSRAQVLQQAGISVLAQANSLPQQVLKLLQ
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Flagellin encoding plasmid DNA was injected into Balb/c mice intramuscularly and this elicited both a humoral and a cellular immune response. Subsequent intravenous challenge of the vaccinated Balb/c mice with 10^5CFU of B. pseudomallei resulted in the number of bacterial cells detected in liver and/or spleen being significantly reduced in the flagellin plasmid DNA vaccinated mice. At 7 days subsequent to infection of B. pseudomallei, 5/6 (83%) of flagellin plasmid DNA vaccinated mice had survived (Chen et al., 2006).
- Related Vaccine(s):
B. pseudomallei DNA vaccine encoding Flagellin Protein
|
8. groEL |
-
Gene Name :
groEL
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei
-
NCBI Protein GI :
AAO46033
-
Other Database IDs :
CDD:237231
CDD:234573 CDD:239460
-
Taxonomy ID :
28450
-
Protein Name :
GroEL
-
Protein pI :
4.86
-
Protein Weight :
50757.59
-
Protein Length :
603
-
Protein Note :
chaperonin GroEL; Reviewed; PRK12850
-
Protein Sequence : Show Sequence
>AAO46033.1 GroEL, partial [Burkholderia pseudomallei]
MAAKDVVFGDSARAKMVEGVNILANAVKVTLGPKGRNVVLERSFGGPTVTKDGVSVAKEIELKDKLQNMG
AQMVKEVASKTSDNAGDGTTTATVLAQSIVREGMKYVASGMNPMDLKRGIDKAVAAAVEELKKISKPCTT
NKEIAQVGAISANSDSSIGDRIAEAMDKVGKEGVITVEDGKSLADELDVVEGMQFDRGYLSPYFINNPDK
QVAVLENPFVLLHDKKVSNIRDLLPVLEQVAKAGRPLLIIAEDVEGEALATLVVNNIRGILKTVAVKAPG
FGDRRKAMLEDIAILTGGQVIAEETGLTLEKATLAELGQAKRIEVGKENTTIIDGAGEAVNIEARVKQIR
TQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVPGGGVALI
RARTAIASLTGVNADQNAGIKIVLRAMEEPLRQIVTNGGEEASVVVAAVAAGKGNYGYNAATGEYVDMVE
AGVVDPTKVTRTALQNAASVAGLLLTTDAAVAELPKEDAPMPGGMPGGM
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Woo et al., 2001)
|
9. LolC |
-
Gene Name :
LolC
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei
-
NCBI Protein GI :
WP_076874059
-
Other Database IDs :
CDD:274034
-
Taxonomy ID :
28450
-
Protein Name :
lipoprotein-releasing ABC transporter permease subunit
-
Protein pI :
9.24
-
Protein Weight :
41576.49
-
Protein Length :
523
-
Protein Note :
lipoprotein releasing system, transmembrane protein, LolC/E family; TIGR02212
-
Protein Sequence : Show Sequence
>WP_076874059.1 lipoprotein-releasing ABC transporter permease subunit [Burkholderia pseudomallei]
MKLPYEWQIGWRYTRAGKRTTGNGFISFIALVSMLGIALGVAALIVVLSVMNGFQKEVRDRMLSVLAHVE
IFSPTGSMPDWQLTAKEARLNRSVIGAAPYVDAQALLTRQDAVSGAMLRGVEPSLEPQVSDIGKDMKAGA
LTALAPGQFGIVLGNALAGNLGVGVGDKVTLVAPEGTITPAGMMPRLKQFTVVGIFESGHYEYDSTLAMI
DIQDAQALFRLPAPTGVRLRLTDMQKAPQVARELAHTLSGDLYIRDWTQQNKTWFSAVQIEKRMMFIILT
LIIAVAAFNLVSSLVMTVTNKQADIAILRTLGAQPGSIMKIFVVQGVTIGFVGTATGVALGCLIASSIPW
LIPMIEHAFGVQFLPPSVYFISELPSELVAGDVIKIGVIAFALSALATLYPSWRGAKVRPAEALRYE
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Harland et al., 2007)
|
10. LolC/E |
-
Gene Name :
LolC/E
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei K96243
-
VO ID :
VO_0010921
-
NCBI Gene ID :
3093214
-
NCBI Protein GI :
53719887
-
Locus Tag :
BPSL2277
-
Genbank Accession :
CP009538
-
Protein Accession :
YP_108873
-
Taxonomy ID :
272560
-
Chromosome No :
1
-
Gene Starting Position :
2742869
-
Gene Ending Position :
2744122
-
Gene Strand (Orientation) :
-
-
Protein Name :
lipoprotein releasing system transmembrane protein
-
Protein pI :
9.43
-
Protein Weight :
41147.07
-
Protein Length :
417
-
Protein Note :
Similar to Escherichia coli lipoprotein releasing system transmembrane protein LolE SWALL:LOLE_ECOLI (SWALL:P75958) (413 aa) fasta scores: E(): 1.3e-42, 34.54% id in 414 aa, and to Ralstonia solanacearum probable lipoprotein releasing system transmembrane rsc1117 or rs05757 SWALL:Q8Y0C7 (EMBL: AL646062) (416 aa) fasta scores: E(): 1.4e-112, 71.46% id in 417 aa
-
DNA Sequence : Show Sequence
>NC_006350.1:2742869-2744122 Burkholderia pseudomallei K96243 chromosome 1, complete sequence
GTCATTCATAGCGCAGCGCCTCCGCCGGGCGCACCTTCGCGCCGCGCCAGCTCGGATAGAGCGTCGCGAG
CGCGGACAGCGCGAAAGCGATCACGCCGATCTTGATCACGTCGCCCGCGACGAGCTCGGACGGCAGCTCG
CTGATGAAGTACACCGACGGCGGCAGGAACTGCACGCCGAACGCATGCTCGATCATCGGAATGAGCCACG
GAATGCTCCACGCGATCAGGCAGCCGAGCGCGACGCCCGTCGCGGTCCCGACGAAGCCGATCGTCACGCC
CTGCACGACGAAGATCTTCATGATCGAGCCCGGCTGCGCGCCGAGCGTGCGCAGGATCGCGATGTCCGCC
TGCTTGTTCGTCACGGTCATCACGAGCGACGACACGAGATTGAACGCCGCGACCGCGATGATCAGCGTGA
GGATGATGAACATCATCCGCTTCTCGATCTGCACCGCGGAGAACCACGTCTTGTTCTGCTGCGTCCAGTC
GCGGATGTACAGGTCGCCCGACAGCGTGTGCGCGAGCTCGCGCGCGACCTGCGGCGCCTTCTGCATGTCC
GTGAGGCGCAGCCGCACGCCCGTCGGCGCGGGCAGCCTGAAGAGCGCCTGCGCATCCTGGATGTCGATCA
TCGCAAGCGTGCTGTCGTATTCGTAGTGCCCGGACTCGAAGATGCCGACCACCGTGAACTGCTTCAGGCG
CGGCATCATCCCGGCCGGCGTGATCGTGCCTTCCGGCGCGACGAGCGTGACCTTGTCGCCGACGCCCACG
CCGAGATTGCCCGCGAGCGCGTTGCCGAGCACGATCCCGAACTGGCCGGGCGCGAGCGCGGTGAGCGCGC
CCGCCTTCATGTCCTTGCCGATGTCGGACACCTGCGGCTCGAGCGACGGCTCGACGCCGCGCAGCATCAC
GCCGCTCACCGCGTCCTGCCGCGTGAGCAGCGCCTGCGCGTCGACATACGGCGCCGCGCCGATCACCGAG
CGGTTCAGGCGCGCTTCCTTCGCGGTCAGTTGCCAGTCGGGCATCGAGCCCGTCGGCGAGAAGATCTCGA
CGTGCGCGAGCACCGACAGCATCCGGTCGCGCACCTCTTTCTGGAAGCCGTTCATCACCGACAGCACGAC
GATGAGCGCCGCGACGCCGAGCGCGATCCCGAGCATCGACACGAGCGCGATGAAGGAAATGAAGCCGTTG
CCGGTCGTGCGTTTGCCGGCGCGCGTGTAGCGCCAGCCGATCTGCCATTCGTACGGGAGTTTCA
-
Protein Sequence : Show Sequence
>YP_108873.1 lipoprotein releasing system transmembrane protein [Burkholderia pseudomallei K96243]
MKLPYEWQIGWRYTRAGKRTTGNGFISFIALVSMLGIALGVAALIVVLSVMNGFQKEVRDRMLSVLAHVE
IFSPTGSMPDWQLTAKEARLNRSVIGAAPYVDAQALLTRQDAVSGVMLRGVEPSLEPQVSDIGKDMKAGA
LTALAPGQFGIVLGNALAGNLGVGVGDKVTLVAPEGTITPAGMMPRLKQFTVVGIFESGHYEYDSTLAMI
DIQDAQALFRLPAPTGVRLRLTDMQKAPQVARELAHTLSGDLYIRDWTQQNKTWFSAVQIEKRMMFIILT
LIIAVAAFNLVSSLVMTVTNKQADIAILRTLGAQPGSIMKIFVVQGVTIGFVGTATGVALGCLIAWSIPW
LIPMIEHAFGVQFLPPSVYFISELPSELVAGDVIKIGVIAFALSALATLYPSWRGAKVRPAEALRYE
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
LolC was evaluated as an antigen for a vaccine candidate against B. pseudomallei infection. Nonmembrane regions of the B. pseudomallei protein was expressed and purified from Escherichia coli and then evaluated as a vaccine candidate in an established mouse model of B. pseudomallei infection. When delivered with the monophosphoryl lipid A-trehalose dicorynomycolate adjuvant, the protein stimulated antigen-specific humoral and cellular immune responses. Immunization with the LolC protein domain afforded significant protection against a subsequent challenge with B. pseudomallei. LolC provided a greater level of protection when it was administered with immune-stimulating complexes complexed with CpG oligodeoxynucleotide 10103. Immunization with LolC also protected against a subsequent challenge with a heterologous strain of B. pseudomallei, demonstrating the potential utility of this protein as a vaccine antigen for melioidosis (Harland et al., 2007).
- Related Vaccine(s):
B. pseudomallei Subunit LolC Protein Vaccine
|
11. ompW |
-
Gene Name :
ompW
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei
-
NCBI Protein GI :
EBA51732
-
Taxonomy ID :
425067
-
Protein Name :
outer membrane protein, OmpW family
-
Protein pI :
6.9
-
Protein Weight :
19075.21
-
Protein Length :
278
-
Protein Note :
identified by match to protein family HMM PF03922; match to protein family HMM TIGR03304
-
Protein Sequence : Show Sequence
>EBA51732.1 outer membrane protein, OmpW family [Burkholderia pseudomallei 305]
MIPSLSHAASPGEGINQGDIIARVRGISIMPDERTSNTLSALNVGVNNAIVPELDFTYMIRDYLGVELIL
GTSRHQITSSLGDLGGVGVLPPTLLLQYHFNHAGKVRPYVGAGINYTLFYNNGLHAGGEGIGINNHSFGP
ALQFGVDVQVTKKVFVNVDVKKIWMHTDATLGGQPLGRLNIDPLVVGVGVGMKF
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Casey et al., 2016)
|
12. oppA |
-
Gene Name :
oppA
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei
-
NCBI Protein GI :
CRY20041
-
Taxonomy ID :
28450
-
Protein Name :
oligopeptide ABC transporter periplasmic oligopeptide-binding protein
-
Protein pI :
8.97
-
Protein Weight :
56354.83
-
Protein Length :
658
-
Protein Sequence : Show Sequence
>CRY20041.1 oligopeptide ABC transporter periplasmic oligopeptide-binding protein [Burkholderia pseudomallei]
MKHTHAFAAVLAALALTIAPSAPAVTVASNVTLADQQDLTRQVPAEVESLDPAHIESWTGNTIGLDLFEG
LARIDASGAVVPGVAQAWEHKAPDTWIFKLRRDAKWSNGQPVTAADFVYAWQRLADPKTGSKYTILVEFV
KNASAIIAGKQPPGDLGIRAIDPYTIEVKTEVPVSYFPELTAMAPLTPVNKDAVAKFGDAWTRPKNIVSN
GPYTLVDWQPNNRIVMAKSDKYWNARNVVIRKVTYLPIENDETALRMYQAGQIDYTYSIPAGGFGQISKQ
FGKELRPGLQLATYYYYLKNSDPALKDKRVREALAMVLDREILTSKITQAGEVPMYGLMPKGVKGVQRPF
TPDWASWPMARRVDYAKNLLKQAGHGDANPLTFTLTYNTNDLHKKVALFAASEWRTKLGVTAKLENVEFK
VLMKQRHDGKVQVARDGWFADYNDAMTFFDLIRCGSSQNTVGYCNPKVDSLVAEANQKLDDGARAALLTQ
AHDLAMNDYPMVPLFQYSADRLVKSYVGGYTLTNYIDMRASQDMYLIKH
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Tippayawat et al., 2009)
|
13. PotF |
-
Gene Name :
PotF
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei K96243
-
VO ID :
VO_0010922
-
NCBI Gene ID :
3095748
-
NCBI Protein GI :
53721504
-
Locus Tag :
BPSS0467
-
Genbank Accession :
BX571966
-
Protein Accession :
YP_110489
-
Taxonomy ID :
272560
-
Chromosome No :
2
-
Gene Starting Position :
636534
-
Gene Ending Position :
637655
-
Gene Strand (Orientation) :
-
-
Protein Name :
ABC transporter substrate-binding protein
-
Protein pI :
6.89
-
Protein Weight :
37606.43
-
Protein Length :
373
-
Protein Note :
Similar to Escherichia coli putrescine-binding periplasmic protein precursor PotF or b0854 SWALL:POTF_ECOLI (SWALL:P31133) (370 aa) fasta scores: E(): 3.3e-67, 49.3% id in 357 aa, and to Pseudomonas aeruginosa polyamine transport protein pa0301 SWALL:Q9I6J0 (EMBL: AE004468) (365 aa) fasta scores: E(): 6.3e-69, 49.59% id in 367 aa. Possible alternative translational start site
-
DNA Sequence : Show Sequence
>NC_006351.1:636534-637655 Burkholderia pseudomallei K96243 chromosome 2, complete sequence
ATCAGTGTCCCGTCTTCAATTGCGCCCACAGACGGTTCTCGAGCCGCAGGATGTCGGCGGGCATCGGCCG
CATCAGCGTCATCTTCTTGAGCACGTCCTCGGGCGGGTAGACGGTCGGGTCCTGCGCGACGGCGGGCACG
ACGAACTGTCGCGCCGCGCGGTTCGCGCTCGGGTAGAACACTTCGTTGGTGATCGCCGCGTTGACCTTCG
GGTCCTCGATGTAGTTGATCCACTTCAGCGCGGCTTCCGCGTGCGGCGCATCCTTCGGGATCACCATCAC
GTCGAACCACAGCAGGCCGCCTTCCTTCGGATTCGAGAACTTGATGTCGTACGGGCGCTTCGCCTCGGCC
GAGCGGCGATGCGCGATGCCGACGTCGCCCGACCAGCCGAGCGCGACGCATACGTCGTTGTTCGCGAGAT
CGTTGATGTAGCCGGACGAGTTGAATTGCGTGATGTACGGGCGGACTTTCTTCAGCACCTCGAACGCCGC
CTGGTAATCGGCCGGGTTCGTGCTGTTCGGATTCCTGCCCATGTATTGTAGCGTCGCGGCGAACACGTCG
ACCGCCTGATCGAGGAACGATACGCCGCAGCCCTTGAGCTTGGATACGTTCGCCGGATCGAGCACGAGCG
CCCAGCTGTCGACGGGCGCGTTCTCGCCGAGCGCCTTCTTCACCGCCTGCACGTTGTAGCCGAGGCCGTC
GGTGCCGAACGCCCACGGCACGCCGTACTGGTTGCCCGGATCGGCGTCGGCGATCATCTTCATCAGCGTC
GGATCGAGGTTCGACAGGTTCGGCAGCTTCGATTTGTCGAGCTTCTGGTAGACGCCCGCCTGAATCTGCT
TGGCCATGTAGTTCGACGTCGGCACGACGATGTCGTAGCCGGAGCTGCCCGCGAGCAGCTTCGCCTGCAG
CGTGTCGTCGCTGTCGTAGTTGTCGTACTTGACGTGGATGCCGGATTGCTTCTCGAAGTTCGGGATCGTG
TCCTTCGCGATGTAGTCCGACCAGTTGTACACGTTCAGCTCGGCGCCGGCCGCGTGCGCGGCCGGCAGGG
CGAGCGCCGACGCGCCGGCGAGCGCGACGAGCGCTGCGCGCGCGACCGCATGACGAAGATGGCTCACACT
CA
-
Protein Sequence : Show Sequence
>YP_110489.1 ABC transporter substrate-binding protein [Burkholderia pseudomallei K96243]
MSVSHLRHAVARAALVALAGASALALPAAHAAGAELNVYNWSDYIAKDTIPNFEKQSGIHVKYDNYDSDD
TLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTLMKMIADADPGNQYGVPWAFG
TDGLGYNVQAVKKALGENAPVDSWALVLDPANVSKLKGCGVSFLDQAVDVFAATLQYMGRNPNSTNPADY
QAAFEVLKKVRPYITQFNSSGYINDLANNDVCVALGWSGDVGIAHRRSAEAKRPYDIKFSNPKEGGLLWF
DVMVIPKDAPHAEAALKWINYIEDPKVNAAITNEVFYPSANRAARQFVVPAVAQDPTVYPPEDVLKKMTL
MRPMPADILRLENRLWAQLKTGH
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
PotF was evaluated as an antigen for a vaccine candidate against B. pseudomallei infection. Nonmembrane regions of the B. pseudomallei protein was expressed and purified from Escherichia coli and then evaluated as a vaccine candidate in an established mouse model of B. pseudomallei infection. When delivered with the monophosphoryl lipid A-trehalose dicorynomycolate adjuvant, the protein stimulated antigen-specific humoral and cellular immune responses. Immunization with the PotF protein domain afforded significant protection against a subsequent challenge with B. pseudomallei (Harland et al., 2007).
- Related Vaccine(s):
B. pseudomallei Subunit PotF Protein Vaccine
|
14. PotF |
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Gene Name :
PotF
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei
-
NCBI Protein GI :
APZ24542
-
Other Database IDs :
CDD:270377
-
Taxonomy ID :
28450
-
Protein Name :
spermidine/putrescine ABC transporter substrate-binding protein PotF
-
Protein pI :
9.52
-
Protein Weight :
38489.12
-
Protein Length :
480
-
Protein Note :
genotype: ST-36
-
Protein Sequence : Show Sequence
>APZ24542.1 spermidine/putrescine ABC transporter substrate-binding protein PotF [Burkholderia pseudomallei]
MKRIAWLAAVLASLACAAARAADGNVLNIYNWAEYFAPDTIADFEKETGIKVRLDVYDSNEALQTKLTTG
NSGYDLVFPSNDFLARQIQAGLYRKLDKSRLPNLTNLDPAIVARAAEVDPGNQYSVPYMQGTFGLGLNIA
KVKQALGGPLPANTLELIFNPAYAAKLERCGIAFNDAGSEVFPLALRYIGRDPNTTDPRDYEAALDMMKK
IRPTIRQFIATPVMNDLATGDVCVVTGYSGAVLVAARRAAEAKNGQQIVYSLPSAGAPFWFDSMAIPKGA
AHADHALRFIDYILRPDVVAKISNKVMYPNPNRVATPLVDRRLTANPAIYPDAATMRTLWVKRPMPPQAM
RMQTRYWTRFKTGY
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Tippayawat et al., 2009)
|
15. purM |
-
Gene Name :
purM
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei K96243
-
NCBI Gene ID :
3092877
-
NCBI Protein GI :
53720427
-
Locus Tag :
BPSL2818
-
Genbank Accession :
BX571965
-
Protein Accession :
YP_109413
-
Taxonomy ID :
272560
-
Chromosome No :
1
-
Gene Starting Position :
3369942
-
Gene Ending Position :
3370997
-
Gene Strand (Orientation) :
-
-
Protein Name :
phosphoribosylaminoimidazole synthetase
-
Protein pI :
4.98
-
Protein Weight :
33766.88
-
Protein Length :
351
-
Protein Note :
Also known as purGcatalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis
-
DNA Sequence : Show Sequence
>gi|53717639:3369942-3370997 Burkholderia pseudomallei K96243 chromosome chromosome 1, complete sequence
GTCAGACCACGACCGTCTGCGCCTCGCCTTCGCGGCTCGCGCGCACGGTGCCGATCTTCCATACCTGCTC
GCCCGCGTCGGCCAGTTGGCGGAGCGCGTCGTCCGCGTCGGCGGCCGACACGATCACGGCCATCCCGATC
CCGCAGTTGAACACGCGGTGCATCTCCGCATCGGCGACGCCGCCGTGCTGCCGCAGCCACTGGAACAGCG
GCGGCAGCGGCCATGCGTGCTGGTCGAGTTCGGCCGTGAGGCCGTCGCGCAGCACGCGCGGAATGTTCTC
GACGAGGCCGCCGCCCGTGATGTGCGCCATTCCCTTCACCGCGATCTTCTCCATCAGCGCGAGGAGCGGC
TTCACGTAGATGCGCGTCGGCGCCATCAGCGCGTCGGCGAGCGAGCGGCCGTGGAAATCGGCCGACAGGT
CCGGATTCGCGCGCTCGATGATCTTGCGCACGAGCGAGAAACCGTTCGAATGGATGCCGCTCGACGCGAG
GCCCAGCACGACGTCGCCCTCGGCGATCGTGCTGCCGTCAATGATCTTGCTCTTCTCGACCGCGCCGACC
GCGAAGCCCGCCAGATCGTATTCGCCGTCCGGGTACATGCCCGGCATCTCGGCCGTCTCGCCGCCGATCA
GCGCGCAGCCCGCCAGCTCGCAGCCCGTCGCGATGCCCTTGACGACGGTCGCGGCCGTCTCGACGTCGAG
CTTGCCGCACGCGAAGTAGTCGAGGAAGAACAGCGGCTCGGCGCCCTGCACGAGAATGTCGTTTACGCTC
ATCGCGACGAGATCCTGGCCGACCGTATCGTGTTTGTTCAGATGAAACGCGAGCTTGAGCTTCGTGCCGA
CGCCGTCCGTGCCCGATACGAGCACGGGCTCCCGGTACTTCTTCGGCACCTCGAACAGCGCGCCGAACCC
GCCGATGCCGCCGAGCACGCCGTCGCGCAGCGTTTTCTTCGCAAAGGGCTTGATCTTGTCGACGAGCGCG
TCGCCCGCGTCGATGTCGACGCCCGCGTCACGGTAGGACAGGCCCTGGGCGTCGGGAGCGGATTTCGGAG
GATTCA
-
Protein Sequence : Show Sequence
>gi|53720427|ref|YP_109413.1| phosphoribosylaminoimidazole synthetase [Burkholderia pseudomallei K96243]
MNPPKSAPDAQGLSYRDAGVDIDAGDALVDKIKPFAKKTLRDGVLGGIGGFGALFEVPKKYREPVLVSGT
DGVGTKLKLAFHLNKHDTVGQDLVAMSVNDILVQGAEPLFFLDYFACGKLDVETAATVVKGIATGCELAG
CALIGGETAEMPGMYPDGEYDLAGFAVGAVEKSKIIDGSTIAEGDVVLGLASSGIHSNGFSLVRKIIERA
NPDLSADFHGRSLADALMAPTRIYVKPLLALMEKIAVKGMAHITGGGLVENIPRVLRDGLTAELDQHAWP
LPPLFQWLRQHGGVADAEMHRVFNCGIGMAVIVSAADADDALRQLADAGEQVWKIGTVRASREGEAQTVV
V
-
Molecule Role :
Virmugen
-
Molecule Role Annotation :
Mice that were challenged with a higher dose of mutant 56:65 purM were protected (Breitbach et al., 2008).
- Related Vaccine(s):
Burkholderia pseudomallei purM mutant vaccine
|
16. purN |
-
Gene Name :
purN
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei K96243
-
NCBI Gene ID :
3092878
-
NCBI Protein GI :
53718549
-
Locus Tag :
BPSL0908
-
Genbank Accession :
BX571965
-
Protein Accession :
YP_107535
-
Taxonomy ID :
272560
-
Chromosome No :
1
-
Gene Starting Position :
1060390
-
Gene Ending Position :
1061052
-
Gene Strand (Orientation) :
?
-
Protein Name :
phosphoribosylglycinamide formyltransferase
-
Protein pI :
6.08
-
Protein Weight :
21138.36
-
Protein Length :
220
-
Protein Note :
glycinamide ribonucleotide transformylase; GAR Tfase; catalyzes the synthesis of 5'-phosphoribosylformylglycinamide from 5'-phosphoribosylglycinamide and 10-formyltetrahydrofolate; PurN requires formyl folate for the reaction unlike PurT which uses formate
-
DNA Sequence : Show Sequence
>gi|53717639:1060390-1061052 Burkholderia pseudomallei K96243 chromosome chromosome 1, complete sequence
GATGAAAAAACTTGTCATCCTGATTTCCGGCCGCGGCAGCAACATGGAGGCCATCGTACGCGCCTGCGCG
CGCGAAGGCTGGCCCGCCGAGGTCGCCGCCGTGATTTCCAACCGGCCGGGCGCGGCCGGCCTCGAATTCG
CGGCGTCGCACGGCATCGCGACGGCCGTCGTCGACCACCGCGCGTTCGACGGCCGCGACAGCTTCGATGC
GGCGCTCGCCGCCGAAATCGACCGCTTCGCCCCCGATCTCGTCGTGCTCGCCGGCTTCATGCGCATTCTC
ACGCCGGCGTTCGTCGCCAAATATGAGGGGCGGATGCTCAACATCCATCCGTCGCTGCTGCCGAGCTTCA
AGGGCATTCATACGCATCAGCAGGCGCTCGACGCGGGCGTCGCGCTGCACGGCGCATCCGTCCACTTCGT
GATTCCGGAGCTCGACAGCGGCGCGATCGTCGCGCAGGCGGCCGTGCCCGTCGTCGCGGGCGACGATGCC
GATGCGCTCGCCGCGCGCGTGCTCGCCGCCGAGCACACGCTGTATCCGCGCGCGGTGCGCTGGTTCGTCG
AAGGCAAGCTGCGCCTCGACGCGGGGCGCGCCATCGTCGCGCCCGACGAGGCGCGCTGGCTGTTCGCGGA
TGCGATCGACACGTCGACGAGCGAGGGCGTATG
-
Protein Sequence : Show Sequence
>gi|53718549|ref|YP_107535.1| phosphoribosylglycinamide formyltransferase [Burkholderia pseudomallei K96243]
MKKLVILISGRGSNMEAIVRACAREGWPAEVAAVISNRPGAAGLEFAASHGIATAVVDHRAFDGRDSFDA
ALAAEIDRFAPDLVVLAGFMRILTPAFVAKYEGRMLNIHPSLLPSFKGIHTHQQALDAGVALHGASVHFV
IPELDSGAIVAQAAVPVVAGDDADALAARVLAAEHTLYPRAVRWFVEGKLRLDAGRAIVAPDEARWLFAD
AIDTSTSEGV
-
Molecule Role :
Virmugen
-
Molecule Role Annotation :
Although 30:93a purN -immunized mice were challenged with a 10-fold higher dose of WT E8, mice were strongly protected and showed no signs of clinical illness until the experiment was terminated (Breitbach et al., 2008).
- Related Vaccine(s):
Burkholderia pseudomallei purN mutant vaccine
|
17. ttsS |
-
Gene Name :
ttsS
-
Sequence Strain (Species/Organism) :
Burkholderia pseudomallei
-
NCBI Protein GI :
WP_004530590
-
Other Database IDs :
CDD:274196
-
Taxonomy ID :
32008
-
Gene Strand (Orientation) :
?
-
Protein Name :
YscQ/HrcQ family type III secretion apparatus protein
-
Protein pI :
6.35
-
Protein Weight :
34946.35
-
Protein Length :
428
-
Protein Note :
type III secretion system apparatus protein YscQ/HrcQ; TIGR02551
-
Protein Sequence : Show Sequence
>WP_004530590.1 MULTISPECIES: YscQ/HrcQ family type III secretion apparatus protein [Burkholderia]
MSARYTLFRRVAPAELPLYRRARALRAAGEHAALRTIAPPRGYAALRATWRGVEHDGWVDLDDLMRRRYP
ALGALAWRALDRRYALDLLSGRDAAAELPAPPGGWAHVRLVDLVERALPAEPLLCFDTPGRARALFRAFP
DEAPAARAVADIGGVPLTMRFAIGTARLPLALLPAVAPGDVLLVCAPRNVVRIGARALCEFCCEGENIML
NEPLADSAIDALHDASHDASHDEPTAAHAPPAFDIDALPVTLEFVLHDERVTVAQLAGCHPGMVLPLRGA
PGEVTIRANGQPFGRGELIQVGEQLAVEVKALWRAKPAACDGE
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Subpopulations of B. pseudomallei-reactive T cells were responsive to BopE, a type III secretion system (TTSS) effector protein, and to a lesser extent to BipD, a TTSS translocator protein.(Haque et al., 2006)
|
III. Vaccine Related Host Genes |
1. Ifng (Interferon gamma) |
-
Gene Name :
Ifng (Interferon gamma)
-
Sequence Strain (Species/Organism) :
Mouse
-
NCBI Gene ID :
15978
-
NCBI Protein GI :
33468859
-
Genbank Accession :
NM_008337
-
Protein Accession :
NP_032363.1
-
Other Database IDs :
MGI:107656; UniProt: P01580
-
Taxonomy ID :
10090
-
Gene Strand (Orientation) :
?
-
DNA Sequence : Show Sequence
>gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
AGTATACCTTTGCAACC
-
Protein Sequence : Show Sequence
>gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
LLPESSLRKRKRSRC
-
Molecule Role Annotation :
IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
-
Additional Molecule Role :
Vaximmutor
-
Additional Molecule Role Annotation :
The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
- Related Vaccine(s):
B. pseudomallei DNA vaccine encoding Flagellin Protein
|
IV. Vaccine Information |
 |
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1. B. pseudomallei DNA vaccine encoding Flagellin Protein |
a. Vaccine Ontology ID: |
VO_0011538 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Gene Engineering of
fliC |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
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e. Adjuvant: complete Freund's adjuvant |
|
f. Vector: |
pcDNA3 (Chen et al., 2006) |
g. Immunization Route |
Intramuscular injection (i.m.) |
h.
Mouse Response |
- Host Strain:
Balb/c
- Vaccination Protocol:
Six-week-old female Balb/c mice from groups 1–3 were injected intramuscularly (i.m.) with pcDNA3/fliC in PBS (50, 100 or 150 μg); whereas those from group 4 (the negative control) were injected (i.m) with vector DNA(150 μg; pcDNA3) in PBS, whilst those from group 5 (the positive control) were injected subcutaneously with recombinant flagellin antigens (50 μg) emulsified in complete Freund's adjuvant. Each animal from group 5 was subsequently boosted with the same dose of flagellin in incomplete Freund's adjuvant 14 days subsequent to the initial flagellin injection. The animals in groups 1–4 were boosted twice with an identical dose of plasmid DNA, once at 7 days and then again at 14 days after the initial injection (Chen et al., 2006).
- Challenge Protocol:
The immunized mice were infected via the tail vein with 105 CFU of mixed B. pseudomallei suspended in sterile PBS (50 μl). As control groups, the mice received either sterile PBS or vector DNA (Chen et al., 2006).
- Efficacy:
Subsequent intravenous challenge of the vaccinated Balb/c mice with 10(5)CFU of B. pseudomallei resulted in the number of bacterial cells detected in liver and/or spleen being significantly reduced in the flagellin plasmid DNA vaccinated mice. At 7 days subsequent to infection of B. pseudomallei, 5/6 (83%) of flagellin plasmid DNA vaccinated mice had survived (Chen et al., 2006).
- Host Gene Response of
Ifng (Interferon gamma)
- Gene Response:
Mice spleen cells were collected after an eight-week immunization. The up-regulation of IFN-γ production among the mice immunized with pcDNA3/fliC was significantly greater than among the mice either immunized with recombinant flagellin or vaccinated with vector DNA only (Chen et al., 2006).
- Detailed Gene Information: Click here.
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2. B. pseudomallei Subunit LolC Protein Vaccine |
a. Vaccine Ontology ID: |
VO_0011436 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Gene Engineering of
LolC/E |
- Type:
Recombinant protein preparation
- Description:
- Detailed Gene Information: Click here.
|
e. Immunization Route |
Intraperitoneal injection (i.p.) |
f.
Mouse Response |
- Host Strain:
BALB/c
- Vaccination Protocol:
Female 5- to 6-week-old BALB/c mice were immunized with the purified recombinant LolC protein given with adjuvant (Harland et al., 2007).
- Challenge Protocol:
Mice immunized with the LolC protein or appropriate controls were challenged with B. pseudomallei K96243 on day 70 (Harland et al., 2007).
- Efficacy:
The recombinant LolC protein afforded significant protection against the B. pseudomallei challenge (Harland et al., 2007).
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3. B. pseudomallei Subunit PotF Protein Vaccine |
a. Vaccine Ontology ID: |
VO_0011438 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Gene Engineering of
PotF |
- Type:
Recombinant protein preparation
- Description:
- Detailed Gene Information: Click here.
|
e. Immunization Route |
Intraperitoneal injection (i.p.) |
f.
Mouse Response |
- Host Strain:
BALB/c
- Vaccination Protocol:
Female 5- to 6-week-old BALB/c mice were immunized with the purified recombinant PotF protein given with adjuvant (Harland et al., 2007).
- Challenge Protocol:
Mice immunized with the PotF protein or appropriate controls were challenged with B. pseudomallei K96243 on day 70 (Harland et al., 2007).
- Efficacy:
Immunization with the PotF protein domain afforded significant protection against a subsequent challenge with B. pseudomallei (Harland et al., 2007).
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4. Burkholderia pseudomallei aroC deletion mutant vaccine |
a. Vaccine Ontology ID: |
VO_0002825 |
b. Type: |
Live, attenuated vaccine |
c. Status: |
Research |
d. Gene Engineering of
aroC |
- Type:
Gene mutation
- Description:
- Detailed Gene Information: Click here.
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e. Preparation |
An unmarked aroC deletion mutant of B. pseudomallei strain A2 was constructed using a sucrose counter-selection strategy. The aroC deletion in the resultant mutant, designated A2DeltaaroC, was confirmed by PCR, Southern hybridization and failure of the mutant to grow in a defined medium without aromatic compound (Srilunchang et al., 2009). |
f. Immunization Route |
Intraperitoneal injection (i.p.) |
g.
Mouse Response |
- Persistence:
Compared to the parental wild type strain, A2DeltaaroC was highly attenuated for virulence following intraperitoneal introduction into BALB/c and C57BL/6 mice (Srilunchang et al., 2009).
- Efficacy:
C57BL/6 mice immunized intraperitoneally with A2DeltaaroC were significantly protected against a challenge dose of 6,000 cfu (20 x LD50) (Srilunchang et al., 2009).
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5. Burkholderia pseudomallei asd mutant vaccine |
a. Vaccine Ontology ID: |
VO_0002826 |
b. Type: |
Live, attenuated vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Mouse |
e. Gene Engineering of
asd |
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f. Immunization Route |
intranasal immunization |
g.
Mouse Response |
- Persistence:
An asd mutant is highly attenuated in mice (Norris et al., 2011).
- Efficacy:
An asd mutant induces protection against challenge with wild type B. pseudomallei (Norris et al., 2011).
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6. Burkholderia pseudomallei purM mutant vaccine |
a. Vaccine Ontology ID: |
VO_0002827 |
b. Type: |
Live, attenuated vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Mouse |
e. Gene Engineering of
purM |
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f. Immunization Route |
Intraperitoneal injection (i.p.) |
g.
Mouse Response |
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7. Burkholderia pseudomallei purN mutant vaccine |
a. Vaccine Ontology ID: |
VO_0002828 |
b. Type: |
Live, attenuated vaccine |
c. Status: |
Research |
d. Host Species as Laboratory Animal Model: |
Mouse |
e. Gene Engineering of
purN |
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f. Immunization Route |
Intraperitoneal injection (i.p.) |
g.
Mouse Response |
- Persistence:
A purN mutant was attenuated in mice, as mice did not show any clinical symptoms of illness (Breitbach et al., 2008).
- Efficacy:
A purN induced strong protection in mice from challenge with wild type B. pseudomallei (Breitbach et al., 2008).
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V. References |
1. Breitbach et al., 2008: Breitbach K, Köhler J, Steinmetz I. Induction of protective immunity against Burkholderia pseudomallei using attenuated mutants with defects in the intracellular life cycle. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2008; 102 Suppl 1; S89-94. [PubMed: 19121696].
2. Casey et al., 2016: Casey WT, Spink N, Cia F, Collins C, Romano M, Berisio R, Bancroft GJ, McClean S. Identification of an OmpW homologue in Burkholderia pseudomallei, a protective vaccine antigen against melioidosis. Vaccine. 2016; 34(23); 2616-2621. [PubMed: 27091689].
3. Chen et al., 2006: Chen YS, Hsiao YS, Lin HH, Yen CM, Chen SC, Chen YL. Immunogenicity and anti-Burkholderia pseudomallei activity in Balb/c mice immunized with plasmid DNA encoding flagellin. Vaccine. 2006; 24(6); 750-758. [PubMed: 16169637].
4. Druar et al., 2008: Druar C, Yu F, Barnes JL, Okinaka RT, Chantratita N, Beg S, Stratilo CW, Olive AJ, Soltes G, Russell ML, Limmathurotsakul D, Norton RE, Ni SX, Picking WD, Jackson PJ, Stewart DI, Tsvetnitsky V, Picking WL, Cherwonogrodzky JW, Ketheesan N, Peacock SJ, Wiersma EJ. Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents. FEMS immunology and medical microbiology. 2008; 52(1); 78-87. [PubMed: 17995960].
5. Gourlay et al., 2015: Gourlay LJ, Thomas RJ, Peri C, Conchillo-Solé O, Ferrer-Navarro M, Nithichanon A, Vila J, Daura X, Lertmemongkolchai G, Titball R, Colombo G, Bolognesi M. From crystal structure to in silico epitope discovery in the Burkholderia pseudomallei flagellar hook-associated protein FlgK. The FEBS journal. 2015; 282(7); 1319-1333. [PubMed: 25645451].
6. Haque et al., 2006: Haque A, Chu K, Easton A, Stevens MP, Galyov EE, Atkins T, Titball R, Bancroft GJ. A live experimental vaccine against Burkholderia pseudomallei elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins. The Journal of infectious diseases. 2006; 194(9); 1241-1248. [PubMed: 17041850].
7. Harland et al., 2007: Harland DN, Chu K, Haque A, Nelson M, Walker NJ, Sarkar-Tyson M, Atkins TP, Moore B, Brown KA, Bancroft G, Titball RW, Atkins HS. Identification of a LolC homologue in Burkholderia pseudomallei, a novel protective antigen for melioidosis. Infection and immunity. 2007; 75(8); 4173-4180. [PubMed: 17517877].
8. Harland et al., 2007: Harland DN, Chu K, Haque A, Nelson M, Walker NJ, Sarkar-Tyson M, Atkins TP, Moore B, Brown KA, Bancroft G, Titball RW, Atkins HS. Identification of a LolC homologue in Burkholderia pseudomallei, a novel protective antigen for melioidosis. Infection and immunity. 2007; 75(8); 4173-4180. [PubMed: 17517877].
9. Nelson et al., 2004: Nelson M, Prior JL, Lever MS, Jones HE, Atkins TP, Titball RW. Evaluation of lipopolysaccharide and capsular polysaccharide as subunit vaccines against experimental melioidosis. Journal of medical microbiology. 2004; 53(Pt 12); 1177-1182. [PubMed: 15585494].
10. Norris et al., 2011: Norris MH, Propst KL, Kang Y, Dow SW, Schweizer HP, Hoang TT. The Burkholderia pseudomallei {Delta}asd mutant exhibits attenuated intracellular infectivity and imparts protection against acute inhalation melioidosis in mice. Infection and immunity. 2011; ; . [PubMed: 21807903].
11. Srilunchang et al., 2009: Srilunchang T, Proungvitaya T, Wongratanacheewin S, Strugnell R, Homchampa P. Construction and characterization of an unmarked aroC deletion mutant of Burkholderia pseudomallei strain A2. The Southeast Asian journal of tropical medicine and public health. 2009; 40(1); 123-130. [PubMed: 19323044].
12. Tippayawat et al., 2009: Tippayawat P, Saenwongsa W, Mahawantung J, Suwannasaen D, Chetchotisakd P, Limmathurotsakul D, Peacock SJ, Felgner PL, Atkins HS, Titball RW, Bancroft GJ, Lertmemongkolchai G. Phenotypic and functional characterization of human memory T cell responses to Burkholderia pseudomallei. PLoS neglected tropical diseases. 2009; 3(4); e407. [PubMed: 19352426].
13. Wiki: B. pseudomallei: Wiki: B. pseudomallei [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei]
14. Woo et al., 2001: Woo PC, Leung PK, Wong SS, Ho PL, Yuen KY. groEL encodes a highly antigenic protein in Burkholderia pseudomallei. Clinical and diagnostic laboratory immunology. 2001; 8(4); 832-836. [PubMed: 11427437].
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