VIOLIN: Vaccine Investigation and Online Information Network

Pathogen Page

Francisella tularensis

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. capB (Protective antigen)
2. dnaK (Protective antigen)
3. FopA (Protective antigen)
4. FopB (Protective antigen, Protective antigen)
5. groEL (Protective antigen)
6. groEL (Protective antigen)
7. IglC1 (Protective antigen)
8. IglC2 (Protective antigen)
9. LpnA (Protective antigen)
10. tul4 (Protective antigen)
11. TUL4 (Protective antigen)
12. WbtI (Protective antigen, Virmugen)
13. capB (Virmugen)
14. clpB (Virmugen)
15. FTL0552 (Virmugen)
16. FTT0918 (Virmugen)
17. galU (Virmugen)
18. guaA (Virmugen)
19. guaB (Virmugen)
20. IglB (Virmugen)
21. IglC (Virmugen)
22. PurC/PurD (Virmugen)
23. purF (Virmugen)
24. purM (Virmugen)
25. SodB from F. tularensis SCHU S4 (Virmugen)
26. sodC (Virmugen)
27. wbtA (Virmugen)
Vaccine Related Host Genes
1. Ccl2
2. Ccl5
3. Cxcl1
4. Ifng (Interferon gamma)
5. IgG
6. Ighg1
7. Ighv1-9
8. IgM
9. IL-1b
10. IL-6
11. Il12a
12. Il12b
13. Il4 (interleukin 4)
14. Nos2
15. TNF-alpha
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

263

2. Disease:

Tularemia

3. Introduction

Francisella tularensis is the etiologic agent of tularemia, a zoonotic disease of which there are several recognized forms depending upon the route of entry of bacteria into the host. These forms include ulceroglandular, glandular, typhoidal, occuloglandular, gastrointestinal and oropharyngeal tularemia. Of these, ulceroglandular tularemia is the most common, with general flu-like symptoms often described. An ulcer at the site of infection is observed, which may persist for up to several months. The lymphatic system disseminates bacteria from the initial infection site and the resultant colonization of the draining lymph nodes leads to swelling; the swollen lymph node resembles the "bubo", a classic symptom of bubonic plague. The most severe form of the disease is the pneumonic form. Primary cases of pneumonic tularemia are generally associated with the disturbance of contaminated dust, for example following the handling of hay or straw which has previously been soiled by infected rodents.

4. Microbial Pathogenesis

There are two types of F. tularensis; Type A is the most virulent causing an often fatal disease while Type B is less virulent and therefore causes a milder form of disease. The species are classified into four distinct sub-species. F. tularensis subspecies tularensis comprises all the strains previously classified as Type A. F. tularensis subspecies holarctica comprises those strains classically referred to as Type B. F. tularensis subspecies mediaasiatica comprises Central Asian strains. The fourth subspecies, F. tularensis subspecies novicida, is a very infrequent cause of human disease, although F. novicida is able to cause a lethal infection in the murine model of disease (Isherwood et al., 2005).

Link to pathogenesis of Francisella tularensis in HazARD.

5. Host Ranges and Animal Models

F. tularensis is the etiologic agent of tularemia, a zoonotic disease that affects a range of animals, including rabbits, hares and rodents. Human disease occurs throughout the northern hemisphere (Isherwood et al., 2005).

6. Host Protective Immunity

While antibody develops against both lipopolysaccharide (LPS) and protein antigens, the role of antibody in protection depends on the nature of the challenge strain. In the murine model of disease, immunization with LPS is able to provide antibody-mediated protection against an otherwise lethal intradermal or intraperitoneal challenge with F. tularensis subspecies holarctica. However, similarly immunized mice are only partially protected against an airborne challenge with F. tularensis subspecies holarctica. When challenged with the most virulent strains of F. tularensis (subspecies tularensis) by any routes, there is at best evidence only of a slight delay in the time to death.

Cellular immunity is likely to play the main role in protection against tularemia. In mice challenged with F. tularensis LVS, neither CD4+ nor CD8+ T cells appear to be absolutely required for control of the infection, but either cell type can promote clearance of the challenge. However, both CD4+ and CD8+ T cells appear to be required for the clearance of a challenge with F. tularensis subspecies tularensis. The clearance of F. tularensis is likely due to the Th1-related cytokines TNFα, IFNγ, and IL-12.

Overall, protection against tularemia is likely to be dependent on the induction of antibody, CD4+ and CD8+ T cell immune responses. The induction of all of these responses appears to be necessary for protection and effective clearance of the most virulent strains (Isherwood et al., 2005).

II. Vaccine Related Pathogen Genes

1. capB

Gene Name : capB
Sequence Strain (Species/Organism) : Francisella tularensis
NCBI Protein GI : ABD95977
Other Database IDs : CDD:188527
Taxonomy ID : 263
Protein Name : CapB
Protein pI : 8.64
Protein Weight : 7876.45
Protein Length : 122
Protein Note : poly-gamma-glutamate synthase PgsB/CapB; TIGR04012

Protein Sequence : Show Sequence

>ABD95977.1 CapB, partial [Francisella tularensis]
KMLWDKLCAKYSGCDKKVLVVNCRDDREDRSKQMAEAALGWQKQDLIVLIGTGTEVFTSFYKKYAKSLN

Molecule Role : Protective antigen
Molecule Role Annotation : (Jia et al., 2010)

2. capB

Gene Name : capB
Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica LVS
NCBI Gene ID : 3952402
NCBI Protein GI : 89256709
Locus Tag : FTL_1416
Genbank Accession : AM233362
Protein Accession : YP_514071
Taxonomy ID : 376619
Gene Starting Position : 1344798
Gene Ending Position : 1346006
Gene Strand (Orientation) : -
Protein Name : capsule biosynthesis protein capB
Protein pI : 7.41
Protein Weight : 41305.26
Protein Length : 402

DNA Sequence : Show Sequence

>gi|89255449:1344798-1346006 Francisella tularensis subsp. holarctica LVS chromosome, complete genome
ACTATAAATTATGCTTCTTTTTATGGCTAGTATCACAGTAATCAACTAATTCCATACCAATATCTTTAAT
ATTACCAACTCCAACAAGAATATAAGAGTTTGCTGGATTTGAATCAACTGTTTTTTCAAGTATTTGTATA
GGTGTCATCTCTTCACAAACTATGACTTTAGTCATTGGTTTATTAAGTGATTTTGCATATTTTTTATAAA
ACGAAGTAAAAACCTCTGTACCAGTACCTATTAGAACAATTAAATCTTGTTTTTGCCAACCTAGAGCTGC
TTCTGCCATTTGTTTTGAGCGATCCTCTCTATCATCTCTACAGTTAACAACCAATACTTTCTTATCACAG
CCTGAATACTTAGCACAAAGCTTATCCCAAAGCATTTTTGTTGATACAGGATCATTAGCAGCAAAAGCAT
TAGCAAAATTTATTTCAGCATTTTTAATATTAAAATTATACTCCGTCATCGCACCTGGATCTGGGGTCGC
TTCCCACATTCCTTTAAGAGCGATCTCTCTAGGTATACCCAAATCATCTGTTACTTTTAATGCTAGAGCA
ACATTTATCTTAAATTCTGAGTATACAAACTTATTTATTTCTTCATCAGATATTTTCTCAGCATCTTGTG
CTGTAGCTGCGATTAGTTCTGAGCCTCGGTCTTTACAAGCATATTCAAAAAAATCTAAATGGATATCTTC
CGCAGTAAAATACTTAGCTCCAACAGGGATAGTAGCTGCTAATGCTTTTGCAACATCTCTTTCTGTAGGA
CCCATAACATCTAAATGATCAGGACGAGCATTTGTCAAAACTCCATGTGTCGCTTTAATAAGCTTTAACT
CACACAGCGATTGCAAAAGTGGCTGCAAAGCCATACACTCGATAACGATAGCATCAGCTTTTGCTCTTCT
TGCCTTAAACATAATCTTAACTTGCTCAGCAATATTACTAAAACCTATTCTAAATACAGGTGTTTCTGAA
CCATCAACATCAATATATCTTGCTAAAGTTCCAGTAGTCTTAGCTACTGTTCTATATCCTCCAGCTCTAA
CACCAGCTGCAATAAGTCTCGCAACACTTGACTTACCACGAGTACCATTGACATGAATCCTAATAGGAAT
ACTTTTAATACTATTATTATGCACTATATTTTCTATTATTAGATAAACGCATAAAATCACAAATACGACG
ACAATTAACCAAAAATCCA

Protein Sequence : Show Sequence

>gi|89256709|ref|YP_514071.1| capsule biosynthesis protein capB [Francisella tularensis subsp. holarctica LVS]
MDFWLIVVVFVILCVYLIIENIVHNNSIKSIPIRIHVNGTRGKSSVARLIAAGVRAGGYRTVAKTTGTLA
RYIDVDGSETPVFRIGFSNIAEQVKIMFKARRAKADAIVIECMALQPLLQSLCELKLIKATHGVLTNARP
DHLDVMGPTERDVAKALAATIPVGAKYFTAEDIHLDFFEYACKDRGSELIAATAQDAEKISDEEINKFVY
SEFKINVALALKVTDDLGIPREIALKGMWEATPDPGAMTEYNFNIKNAEINFANAFAANDPVSTKMLWDK
LCAKYSGCDKKVLVVNCRDDREDRSKQMAEAALGWQKQDLIVLIGTGTEVFTSFYKKYAKSLNKPMTKVI
VCEEMTPIQILEKTVDSNPANSYILVGVGNIKDIGMELVDYCDTSHKKKHNL

Molecule Role : Virmugen
Molecule Role Annotation : There was a deletion in gene capB of F. tularensis live vaccine strain. LVS capB, while much less virulent than LVS, induces potent protective immunity against lethal LVS i.n. challenge in mice, comparable to that induced by LVS (Jia et al., 2010).
Related Vaccine(s): Francisella tularensis capB mutant vaccine

3. clpB

Gene Name : clpB
Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
NCBI Gene ID : 3192509
NCBI Protein GI : 56708764
Locus Tag : FTT_1769c
Genbank Accession : AJ749949
Protein Accession : YP_170660
3D structure: PDB ID : 3P2L
Taxonomy ID : 177416
Gene Starting Position : 1857727
Gene Ending Position : 1860306
Gene Strand (Orientation) : -
Protein Name : ClpB protein
Protein pI : 5.27
Protein Weight : 89876.23
Protein Length : 859
Protein Note : Similar to FASTA: AAO89660 (Q83F55) ClpB protein from Coxiella burnetii (859 aa). FASTA: opt: 3593 Z-score: 3223.6 E(): 1.1e-171 Smith-Waterman score: 3593; 65.357 identity in 840 aa overlap

DNA Sequence : Show Sequence

>gi|255961454:1857727-1860306 Francisella tularensis subsp. tularensis SCHU S4, complete genome
TTTATTTAGAGAATATAATATTGTTATTAGCGTCAATATCGACAACTATTTTATCTTCAGCTTTAAACTC
ACCATCTAGAAGTTTTAGAGCCAGAGGGTTTTCTAAGTTGTTTTGAATAGCGCGCTTAAGCGGTCTAGCA
CCAAACACAGGATCAAAGCCAGCATCAGCTAGTTTATCCATAGCTCCAGTTGTGACTTCTAATCCTATAC
TTAGATCTGCTAGACGTTTTTCTAAGCGTTTGATTTGTATTTTAGCTATTTCAGTTATCATCTCTTTGTT
TAGAGGTTCAAAGACAATTGCATCATCAACTCTATTTACAAATTCAGGTCTAAAGTGGCTAAGTACCATT
TCCATTACAGCAGATTTTACTGTTTCATAATCCTGACCTTGCATTTCTTGGATTCGATGTGAACCAAGGT
TAGAAGTCATAACTATTACAGTATTTTTAAAGTCTACTGTTCTGCCTTGCCCATCTGTTAGGCGACCATC
GTCAAGCACTTGTAGTAAGATGTTAAATATATCAGCATGAGCTTTTTCAACCTCATCAAGTAAGATTACA
GAATAAGGTTTTCTTCTAACATGTTCAGTTAGATAGCCGCCTTGTTCATAACCTACGTATCCAGGAGGTG
CGCCTATTAGTCTAGCTACAGAATGTTTCTCCATAAACTCAGACATATCTACTCTAAGCATGGCATCTTC
ATCATCAAACAAAAACTCTGCTAAAGCTTTTGTAAGCTCAGTTTTACCGACACCAGTTGGACCTAAGAAC
ATGAATGAGCCTATAGGTCTATTTGGATCTGATAATCCAGAACGAGATCTTCTTACAGCATTTGATACTG
CTTTTATAGCTTGATCTTGTCCGATTACTCTTTTATGTAAGAAACTTTCCATATTTAGAAGCTTGTCTTT
TTCGCCTTCCATCATCTTAGACACAGGTATTCCAGTAGCTTTTGAAACTACATCAGCAATCTCATTTTCT
GTAACAGATGTTCTTACTAATTTGTTTTCAGAAGGTTCTGCTTCAGTTTCTTCTATTTGTTTAATTTGTG
CTTCTAGCTCAGGTATTTTACCGTATTGTAATTCTGCCATTTTGCTCAAATCACCCACTCTTTGGTACTT
TTCAAGTTCAAACTTGGCTTTCTCAAGCTCTTCTTTTAGTTTACTTGTACCTTGCATCTTAAGCTTTTCA
GCTTTCCAAAGCTCTTCTAGTCCTTTATACTCGGAGTCTAGCCCTTTTATTTCTTGCTCAAGTATCTCTA
AACGTTTTTTAGTAGCATCATCTTTCTCTTTCTTTAGCTGTTCGCGTTGCATTTTCAGCTGGATGATTCT
ACGATATAAGCTTTCCATTTTTTCAGGTTTAGAGTCTATTTCCATACGAATTTGGCTTGCTGCTTCATCT
ACTAGATCTATAGCTTTATCAGGTAGCTGTCTATCTGTGATATACCTATGTGACAAAGTTGCTGCACTTA
CAATAGCTGAATCTGTGATATTTACACCATGATGTAGCTCATATCTTTCTTTTAGGCCTCTAAGTATAGC
GATAGTATCTTCTACAGTAGGTTCATCAACTAGCACTTTCTGGAATCTTCGCTCAAGTGCCGGATCTTTT
TCAACATACTCACGATACTCATCTAAAGTTGTTGCACCAACACACTTTAGCTCTCCTCTAGCTAGAGCAG
GTTTAAGCATGTTACCAGCATCCATAGATCCTTCTGCTTTACCAGCACCTACCATAGTATGTAATTCATC
TATAAAGAGAATTACATTACCTTCTTGTTTTGATAACTCTTTTAATACAGATTTTAGACGCTCTTCAAAA
TCTCCTCTAAATTTAGCACCAGCTAGCAGTGCACCCATATCTAATGATAGTACTTTTTTACCTTTGACAC
CTTCTGGTACTTCATCATTAACTATTCGTTGAGCTAAGCCCTCAACAATAGCAGTTTTACCAACACCAGG
CTCACCTATAAGCACAGGGTTGTTCTTAGTTCTTCTTTGTAATACTTGAATAGTTCTACGGATCTCACTA
TCTCTACCGATTATTGGATCAATTTTTCCTTTTCTCGCTAGATCAGTTAGATCTACCGTGTATTTATCTA
ATGCACCTTTCATATCTTCTTGATTTTGACTACTCACTTTCTCCCCTCCGCGATAATCATTGACTGCTTT
TGTTAATTTTTCTTTTGTAATACCAAATTTGTTATACAGTCCAGTTAAACTTTTGTCTTCTAAAGAGGCT
AATAAGAAAACCTCACTCGAGATAAACTCATCACCATTTTTATTAGCAAGCCCTTGCATTTTATGTAATG
TAGCTATTAAATCTCTAGATGGTGTTACTTGAGGGTTACCTTCTCCAGATAATACCGCAACACTATCAAC
CATATCATTTACAGCTTTTATAAAGTTTTGTATATTAACACCACAAACGCTTAATATAGATATAGCAACA
CTATCATTTTGCTCCAAAAGAGCTTTTAGTATATGTGCTGATGTAAACTCAGTTGCTTTTTGTTGAAAAG
CATAAGATTGAGCCTCAGCTAGAGCTTCTTGTAGTTTTATTGTAAATTTATTTATATCCA

Protein Sequence : Show Sequence

>gi|56708764|ref|YP_170660.1| ClpB protein [Francisella tularensis subsp. tularensis SCHU S4]
MDINKFTIKLQEALAEAQSYAFQQKATEFTSAHILKALLEQNDSVAISILSVCGVNIQNFIKAVNDMVDS
VAVLSGEGNPQVTPSRDLIATLHKMQGLANKNGDEFISSEVFLLASLEDKSLTGLYNKFGITKEKLTKAV
NDYRGGEKVSSQNQEDMKGALDKYTVDLTDLARKGKIDPIIGRDSEIRRTIQVLQRRTKNNPVLIGEPGV
GKTAIVEGLAQRIVNDEVPEGVKGKKVLSLDMGALLAGAKFRGDFEERLKSVLKELSKQEGNVILFIDEL
HTMVGAGKAEGSMDAGNMLKPALARGELKCVGATTLDEYREYVEKDPALERRFQKVLVDEPTVEDTIAIL
RGLKERYELHHGVNITDSAIVSAATLSHRYITDRQLPDKAIDLVDEAASQIRMEIDSKPEKMESLYRRII
QLKMQREQLKKEKDDATKKRLEILEQEIKGLDSEYKGLEELWKAEKLKMQGTSKLKEELEKAKFELEKYQ
RVGDLSKMAELQYGKIPELEAQIKQIEETEAEPSENKLVRTSVTENEIADVVSKATGIPVSKMMEGEKDK
LLNMESFLHKRVIGQDQAIKAVSNAVRRSRSGLSDPNRPIGSFMFLGPTGVGKTELTKALAEFLFDDEDA
MLRVDMSEFMEKHSVARLIGAPPGYVGYEQGGYLTEHVRRKPYSVILLDEVEKAHADIFNILLQVLDDGR
LTDGQGRTVDFKNTVIVMTSNLGSHRIQEMQGQDYETVKSAVMEMVLSHFRPEFVNRVDDAIVFEPLNKE
MITEIAKIQIKRLEKRLADLSIGLEVTTGAMDKLADAGFDPVFGARPLKRAIQNNLENPLALKLLDGEFK
AEDKIVVDIDANNNIIFSK

Molecule Role : Virmugen
Molecule Role Annotation : A clpB mutant in Francisella tularensis subspecies tularensis strain SCHU S4 was attenuated and provided protection against aerosol challenge in mice (Conlan et al., 2010).
Related Vaccine(s): Francisella tularensis clpB mutant vaccine

4. dnaK

Gene Name : dnaK
Sequence Strain (Species/Organism) : Francisella tularensis
NCBI Protein GI : AAA69561
Other Database IDs : CDD:234715
CDD:302596
CDD:212657
Taxonomy ID : 263
Protein Name : DnaK
Protein pI : 4.64
Protein Weight : 64735.94
Protein Length : 695
Protein Note : molecular chaperone DnaK; Provisional; PRK00290

Protein Sequence : Show Sequence

>AAA69561.1 dnaK [Francisella tularensis]
MGKIIGIDLGTTNSCLAIMDGKTAKVIENAEGHRTTPSVVAYTDSGEILVGQAAKRQAVTNPDNTFFAIK
RLIGRKYDDKAVQEDIKKKVPYAVIKADNGDAWVATKEGKKMAPPQVSAEVLRKMKKTAEDYLGEPVTEA
VITVPAYFNDSQRQATKDAGKIAGLEVKRIINEPTAAALAYGVDSKKGEQTVAVYDLGGGTFDISIIEIS
DVDGDNQIEVLSTNGDTFLGGEDFDLALMNYLIDEFKKEQGIDLHNDKLALQRVREAAEKAKVELSPAQQ
TDVNLPYITADATGPKHLNIKVTRAKFESLVSDLVMRSLEPCKKALEDAGLSKSDITEVLLVGGQTRMPL
VQEKVKEFFGKEPRKDVNPDEAVAVGAAIQGGVLAGDVKDVLLLDVTPLSLGIETMGGVMTKLIERNTTI
PTKKSQVFSTAEDNQPAVTIHVLQGEREMASANKSLGRFDLADIPPAPRGMPQIEVTFDIDANGILNVSA
KDKATGKEQNIVIKSSSGLSEEDIEKMVQDAEANAEADKKFHDLVTARNTADNLIHSSRKAIQELGDKVT
AAEKEKIEEACKELEAATKGDDKQAIEAKTKALEEAFAPIAQKAYAEQAQAAGAQGGAKAEEPKKEEDVV
DADFEDVEDDKK

Molecule Role : Protective antigen
Molecule Role Annotation : (Ashtekar et al., 2012)

5. FopA

Gene Name : FopA
Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica OSU18
VO ID : VO_0011078
NCBI Gene ID : 4306351
NCBI Protein GI : 115315051
Locus Tag : FTH_1293
Genbank Accession : CP000437
Protein Accession : YP_763774
Taxonomy ID : 393011
Gene Starting Position : 1267837
Gene Ending Position : 1269018
Gene Strand (Orientation) : -
Protein Name : outer membrane protein FopA
Protein pI : 5.43
Protein Weight : 39079.73
Protein Length : 393

DNA Sequence : Show Sequence

>NC_008369.1:1267837-1269018 Francisella tularensis subsp. holarctica OSU18, complete genome
ATTAGTTAGCTTCTTTAAGTGGAGCTGATACGCTAGCTTCTACACGCTGGTTACGCGGATCAGACTTATG
AATACCACCTAAAGTATCATTATAGCCAAAACCTTTAGTAATTATTTTCTCACTATCTAAACCTAATTGC
TTCATGTAATCAGCAACAGCTTGTGCTCTCTTCTCAGAAAGTTTTTGGTTATATATATCAAACTCACTAC
CAGTTTGACCTTGAGAAGCATAACCTTTAACTGTTACAGAGCTAATGTTACTATCCTTGATAAAATTAAC
AAAAGATGCAATAGCTTTTTTACCTTTAGCATTTAAAGTAGCTTTATCATAAGCAAACTTAGTATCTAGG
TAAACTGTTACATCATCACCATTTACTGTATAGCACGCGACACCATCTTCAGTTAGATTAAAGTTGCCTT
CACATTGCTTAATACCAGCAGGTAAAACATACTTAGACTCATCAATAGTTGGCATAGCAACAGTTTGAGC
AGCTGTAGTCGCACCATTATCCTGAATATTACCAGTGTCATTATTAGTAGTATCTTTGCCACCAAAGAAC
CATGTTAAACCAGCACCAATCATGTTAGTACCCGCTCTGCCATTAGCACCAGAAATATTACTAGGTGCCA
TTGTTTGGATATATCTGTAGTCAACACTTGCTTGAACATTTCTAGATAGTTCAAACTTAAGACCACCACC
CACATCCCAAGCACCTGTTGCTTGACCTGCTAGATTTGCCCAACCAGCACCACCTGCAGCATATGGAGTA
AACATAGTATTATTACTAAAGTTTACAACACCTTCACCTAAACCAGCAAATACTCTACCAACTAATTGGT
TGTACTGTACAGCGAAGTATTTATTGATGTTATAACCGATTGTGAAGTTAGCACCAGCACCTGATGGAGA
GTTAGGAGTTCCAGCTAGACCGTTAGCATCTACACCTAAGTACCACTGGCCAGTTCTATCTTGAGGACCC
CAAGTATTGTCCTTGTTAGTCAAAGCGCTATAAGTATTTGCAAAAGGAGCAATGAAATTATTAGCTGCAA
AACCACTGCTTTGTGTAGTAGCTGAATTAGAGTTTGCTGACGTATCGATATTATCTGAACCTGCAGCGAT
AGATGCTGTAGCTGAACCTAATAATACAGTTGTAGCTATAACAATACTTTTTAATCTCATCA

Protein Sequence : Show Sequence

>YP_763774.1 outer membrane protein FopA [Francisella tularensis subsp. holarctica OSU18]
MMRLKSIVIATTVLLGSATASIAAGSDNIDTSANSNSATTQSSGFAANNFIAPFANTYSALTNKDNTWGP
QDRTGQWYLGVDANGLAGTPNSPSGAGANFTIGYNINKYFAVQYNQLVGRVFAGLGEGVVNFSNNTMFTP
YAAGGAGWANLAGQATGAWDVGGGLKFELSRNVQASVDYRYIQTMAPSNISGANGRAGTNMIGAGLTWFF
GGKDTTNNDTGNIQDNGATTAAQTVAMPTIDESKYVLPAGIKQCEGNFNLTEDGVACYTVNGDDVTVYLD
TKFAYDKATLNAKGKKAIASFVNFIKDSNISSVTVKGYASQGQTGSEFDIYNQKLSEKRAQAVADYMKQL
GLDSEKIITKGFGYNDTLGGIHKSDPRNQRVEASVSAPLKEAN

Molecule Role : Protective antigen
Molecule Role Annotation : Anti-FopA MAbs protected 90% of naïve C3H/HeN mice from lethal challenge with F. tularensis LVS when administered prophylactically as well as therapeutically (Savitt et al., 2009).

6. FopB

Gene Name : FopB
Sequence Strain (Species/Organism) : Francisella novicida U112
VO ID : VO_0011070
NCBI Gene ID : 4547777
NCBI Protein GI : 118496734
Locus Tag : FTN_0119
Genbank Accession : CP000439
Protein Accession : YP_897784
Taxonomy ID : 401614
Gene Starting Position : 130543
Gene Ending Position : 131082
Gene Strand (Orientation) : +
Protein Name : hypothetical protein
Protein pI : 4.55
Protein Weight : 18246.3
Protein Length : 179

DNA Sequence : Show Sequence

>NC_008601.1:130543-131082 Francisella tularensis subsp. novicida U112, complete genome
TATGAAAAAAACTATACTAGGAGCAATGATCGCTGGTGGTTTGATGGTTTCTGCAACTACAGCTATGGCT
GGTGGGGTAGGTTTTGCTAATGTCCAAGATATTTTTGAAACTTCACCTCTTGGTAAAGCAAAGGTAACAG
CTGATGAGAAAAAGCTAAAGCCACAGATGGACCAACTTAAGCAAAACATCACTGCTTTACAAGAGAAAGT
AAATGCATACACTCAAGAAAAAGATGATGTTCAAACAGATGATTCTAAAGGCGATACTAAAGATGCACAG
TCAGCAGACAAAGTAGAAAATCAAGATAAGCAACAAGCTCAAGCAGATCTTGAAAAAGCAATGAAAGATT
ACCAAAACCTAATGAATCAGGTTCAAAAAATGGCTTCTGACGATGCTGATGCGTTCAAAGATGCTTTGAC
TAAGGCTTCTGCTCAAGTTGCTAAAGAGAAGCAACTTGATGCTATTTTACCTGCTGAAATGAGTCTATAT
AATGTTGATAGTATTGATGTTACTAAAGATGTTATAGCTAAGATGCAATA

Protein Sequence : Show Sequence

>YP_897784.1 hypothetical protein FTN_0119 [Francisella tularensis subsp. novicida U112]
MKKTILGAMIAGGLMVSATTAMAGGVGFANVQDIFETSPLGKAKVTADEKKLKPQMDQLKQNITALQEKV
NAYTQEKDDVQTDDSKGDTKDAQSADKVENQDKQQAQADLEKAMKDYQNLMNQVQKMASDDADAFKDALT
KASAQVAKEKQLDAILPAEMSLYNVDSIDVTKDVIAKMQ

Molecule Role : Protective antigen
Molecule Role Annotation : Intranasal immunization of HLA-DR4 tg mice with the single antigen FopB from F. tularensis subsp. novicida conferred significant protection against lethal pulmonary challenge with an F. tularensis subsp. holarctica live vaccine strain (Yu et al., 2010).
Additional Molecule Role : Protective antigen
Related Vaccine(s): F. tularensis FopB Protein Vaccine

7. FTL0552

Gene Name : FTL0552
Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica LVS
NCBI Gene ID : 3952730
NCBI Protein GI : 89255956
Locus Tag : FTL_0552
Genbank Accession : AM233362
Protein Accession : YP_513318
Taxonomy ID : 376619
Gene Starting Position : 534315
Gene Ending Position : 535001
Gene Strand (Orientation) : ?
Protein Name : two-component response regulator
Protein pI : 6.31
Protein Weight : 23733.73
Protein Length : 228

DNA Sequence : Show Sequence

>gi|89255449:534315-535001 Francisella tularensis subsp. holarctica LVS chromosome, complete genome
TATGAGAATATTGTTGGCTGAAGATGATCTTCATTTGGGTGAAGGCTTATTAGAAGCTTTGCAAAAAGAG
GGTTTGATTGTAAATCTAGTTTCAGATGGTGAGGCAGCGCAAACTTTTATAGAATCTGGATTGTATGATA
TAGTCGTCTTAGATATTGGTATGCCAATAAAAACTGGTTTAGAAGTTCTAAGAAATATTAGAAATAGAGG
TATTAAAGTACCAATAATACTGTTGACTGCTAGAGATGGTTTAGAAGATAGAATTAAAGGTCTTGATCTT
GGTGCTGATGATTATCTAACTAAGCCATTTGAGTTAAAAGAGCTAGTAGCTAGAATTAAAGCTATTTCTC
GTCGTATTGATACTAGATCTGGTAAAAGTGTTAATGAAGAAATTAGATTTGGAGATTATAGTTTTAATCC
AAGCTCTGAAACGGTAACCAAAGATGGAGTTATTATTCCTGTTTCAAAAAAAGAGTTAGCGCTTTTAGCT
ATATTGGTACAAAATGCTGGTAGGGTAGTACCAAAGACACAGCTTTTAGAAGAAATATATTCTACTGATA
AGGAAATGGATACAAACACCCTAGAAGTACATATGCATAATTTAAGAAAGAAAATTAACATTCCTAATTT
TATACAGACTATTAGAGGTGTTGGTTACTTTGTACAAAAGGATAAAGTAATTAAGTA

Protein Sequence : Show Sequence

>gi|89255956|ref|YP_513318.1| two-component response regulator [Francisella tularensis subsp. holarctica LVS]
MRILLAEDDLHLGEGLLEALQKEGLIVNLVSDGEAAQTFIESGLYDIVVLDIGMPIKTGLEVLRNIRNRG
IKVPIILLTARDGLEDRIKGLDLGADDYLTKPFELKELVARIKAISRRIDTRSGKSVNEEIRFGDYSFNP
SSETVTKDGVIIPVSKKELALLAILVQNAGRVVPKTQLLEEIYSTDKEMDTNTLEVHMHNLRKKINIPNF
IQTIRGVGYFVQKDKVIK

Molecule Role : Virmugen
Molecule Role Annotation : A FTL0552 mutant in Francisella tularensis was attenuated and provided partial protection in mice against challenge with a lethal dose of the virulent SchuS4 strain when compared to naive mice (Sammons-Jackson et al., 2008).
Related Vaccine(s): Francisella tularensis FTL0552 mutant vaccine

8. FTT0918

Gene Name : FTT0918
Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
NCBI Gene ID : 3191684
NCBI Protein GI : 56708019
Locus Tag : FTT_0918
Genbank Accession : AJ749949
Protein Accession : YP_169915
Taxonomy ID : 177416
Gene Starting Position : 927665
Gene Ending Position : 929338
Gene Strand (Orientation) : ?
Protein Name : hypothetical protein
Protein pI : 4.53
Protein Weight : 55767.38
Protein Length : 557
Protein Note : ORF ftt0918

DNA Sequence : Show Sequence

>gi|255961454:927665-929338 Francisella tularensis subsp. tularensis SCHU S4, complete genome
TGTGGTGCGTAAATTTAAAAAAACCTGTTTGATAGTTAGTAGTTTATTGGCTTGTAGTGGTTTAGCTTAT
TCTGAAGATTCTCCTCAAGTTGTTTCACAAGGGGGGCCTCTTGGAGCTACTAGTATTGGTGATCAAAACC
TTGGACAGCCAGATCCAAATGCTAGTGGAGCCTCTTCAACAACACAGACTACCGGTTCAAATCTAAATGA
TAGGGAACTTTTGCTAAAATTACAGCAGCAGGTACAGCAACTTCAGGGACAATTACAACAGCTAAAAGCA
CAGGGTAATGGTGGTGGATTACAGAATACCTATAATGGTAGTTCGCAGTTTACTACTTACAGCTCAAAAG
TTGATGGTAATAAAAATCCTCGTACGCTTGGAGGCAATGGTGAGAGTAAAGATCTGAGTCAGGCTTTGAT
TGGTGGTCAAACGTCGTCAGATATTATGGGGAATGTTAATGCTAGTAACTCTATCATTAATTTAGCTTCT
GAGCCATTAGGAGGCGTCTTTAACCAAAAAGGCGGTATCGACGTTGGTGGAGCTCCGGCGATTACAACAC
AAGGTCAAGTTACCTACTTAGGTTCGTACTCTGGTAACAACAGTATTCCAATTGGTCAGATTTCTTCTAA
CCTTTTTGCTTCTACATTGTTGGGTCAAAGAGAGAAGTTTGATGACTACTCTGTATTCTTTGGTGGCTTT
ATAGAAGCAGATGCCCAAGCTTGGTTTGGTAGTGCTGTTACTAAGGTGCAAAATGCTGGCCAGTTATCTA
GCAATGGCCAAAATATATATTTAACATCAGCTAATTTATATTTCTTATCAAATCTTGGTCATTATGTAAC
AGCTCAGTTTGATTTTGATACTAATGAGTCAGGAAGTTTTAGTTTAGGTAATGCTTTTGTAATTTTTGGT
AACTTAGATATATCACCATTCTTCGTAACAGCAGGTAGAAACAAGCTATCTGTTGGCTCATATGGTGGTG
GTGGTACTTGGACTAGCGGTATCACCAAATTTCTATCACCAAATCAGGTTACTAACGTATCTATTGACTA
TAAAGATCAAGTCTGGAACGCCAACATTGCAGTATTTGGCTCTGATGATAGACGTGCAAACTTCTCAACA
GGTTTATTCTATGCTGATAGCTGGACACCAAACTTAGCGGCTGGTTTTAACGTAGGTTATGTCTTTAATA
TTGCTGGTGCTGGTAACTCTTCGATTGCTAACTCATTAGCTAACTTAAATCGTAGTAGTGATAATGTGGG
AGCTTTAAACGTTGACGGCAACTTAACTTATGCAATTTGGGATGGATTTTTAAACTTAGGAGCAGGTTGG
GCTAGTACTACGACAAAAGAAGATTTTAATAATAATGGTGGTAGTGTACTTGCTGGGGCATGGTATGGAG
CACTTAACTATTCTGCGATACTTGGTGGTAGAAATACTAACTTCGGTGTGACTTATGGTCAATCATATAA
TGCTGCAGCTATCCCAATGGAGACAGCAAATGCTTCACCAACTTTCGGTCAAACAGCATCTGGTATCAAA
CAGCAACTTATCTTCTCGGCTCAGCGAGCTTACTTTGATGACAATGTTCTATTTGGTCCTGAATATGCGT
ATCAAAGACTATATACTGGCGAACATATGAATACAATTACTCTGGATATGTCGGTATACGTATA

Protein Sequence : Show Sequence

>gi|56708019|ref|YP_169915.1| hypothetical protein FTT_0918 [Francisella tularensis subsp. tularensis SCHU S4]
MVRKFKKTCLIVSSLLACSGLAYSEDSPQVVSQGGPLGATSIGDQNLGQPDPNASGASSTTQTTGSNLND
RELLLKLQQQVQQLQGQLQQLKAQGNGGGLQNTYNGSSQFTTYSSKVDGNKNPRTLGGNGESKDLSQALI
GGQTSSDIMGNVNASNSIINLASEPLGGVFNQKGGIDVGGAPAITTQGQVTYLGSYSGNNSIPIGQISSN
LFASTLLGQREKFDDYSVFFGGFIEADAQAWFGSAVTKVQNAGQLSSNGQNIYLTSANLYFLSNLGHYVT
AQFDFDTNESGSFSLGNAFVIFGNLDISPFFVTAGRNKLSVGSYGGGGTWTSGITKFLSPNQVTNVSIDY
KDQVWNANIAVFGSDDRRANFSTGLFYADSWTPNLAAGFNVGYVFNIAGAGNSSIANSLANLNRSSDNVG
ALNVDGNLTYAIWDGFLNLGAGWASTTTKEDFNNNGGSVLAGAWYGALNYSAILGGRNTNFGVTYGQSYN
AAAIPMETANASPTFGQTASGIKQQLIFSAQRAYFDDNVLFGPEYAYQRLYTGEHMNTITLDMSVYV

Molecule Role : Virmugen
Molecule Role Annotation : An FTT0918 mutant showed significantly reduced virulence in mice. Mice that recovered from infection with the mutant were protected 2 months later from challenge with wild type F. tularensis (Twine et al., 2005).
Related Vaccine(s): Francisella tularensis FTT0918 mutant vaccine

9. galU

Gene Name : galU
Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
NCBI Gene ID : 3191600
NCBI Protein GI : 56707872
Locus Tag : FTT_0757
Genbank Accession : AJ749949
Protein Accession : YP_169768
Taxonomy ID : 177416
Gene Starting Position : 776923
Gene Ending Position : 777786
Gene Strand (Orientation) : ?
Protein Name : UTP--glucose-1-phosphate uridylyltransferase
Protein pI : 7
Protein Weight : 30262.51
Protein Length : 287
Protein Note : Similar to Q9K0G7 UTP--glucose-1-phosphate uridylyltransferase from Neisseria meningitidis (serogroup B) (289 aa). FASTA: opt: 1017 Z-score: 1291.1 E(): 5e-64 Smith-Waterman score: 1017; 57.439 identity in 289 aa overlap.

DNA Sequence : Show Sequence

>gi|255961454:776923-777786 Francisella tularensis subsp. tularensis SCHU S4, complete genome
AATGAAAATAAGAAAAGCAGTTTTTCCTGTAGCTGGATGGGGCACTAGATTTTTACCAGCTACTAAATCA
TGTCCTAAAGAAATGCTAACAGTTGTTGATAAACCATTGATCCAATATGCTGTTGAAGAGGCTATAGAGG
CAGGCTGTAAAGAGATAATATTTGTTACTAGCTCAAATAAAAAATCCCTAGAAGATCATTTTGATAGAAA
CTTTGAATTAGAATATTCGCTAGAAAAAAAACAAAAATATGAATTGTTAGATTTAGTAAAAAATATAATT
CCTAAAGATGTAAGTTTTTTCTTTGTACGTCAGCCAGAGGCGCTAGGACTAGGGCATGCGGTTTTATGTG
CAAAACCACTAGTTGGAATTGAGGATTTTGCAGTTATATTACCTGATGATTTAATTTACAATCATGATTG
TGGTACTGGTACGCTAAAGCAAATGGTTAAGGCGGTAGAAGGTACTGATATTAGAGGTTGTATCGCAACT
CAACAAGTCAAGAGAGAAAAAACAAATTCATATGGTATAGTTGCAAAAGATAATGATAATCTAATCAAAG
CAATTGTTGAAAAGCCAGCACCAGAAAAAGCACCATCAACTAATGCAGTTGTGGGTAGATATTTATTACC
AAATAAGATTTTTAGATGTTTAGAATCAACTTCAGAGGGCGCAGGTGGTGAGATTCAACTAACTGATGCT
ATCGCTAAATTATTAGATCAGGATGAGAAAATTTTGTCTTATGAATTTAAGGGTACTCGTTATGATTGTG
GCAGTAAATTAGGTTTTTTAATTGCTAATTATGAGATAGCTTTACAGCATCAAGAGTTAGGACATAAATT
CAAAGAATACCTTCAAAATAGATA

Protein Sequence : Show Sequence

>gi|56707872|ref|YP_169768.1| UTP--glucose-1-phosphate uridylyltransferase [Francisella tularensis subsp. tularensis SCHU S4]
MKIRKAVFPVAGWGTRFLPATKSCPKEMLTVVDKPLIQYAVEEAIEAGCKEIIFVTSSNKKSLEDHFDRN
FELEYSLEKKQKYELLDLVKNIIPKDVSFFFVRQPEALGLGHAVLCAKPLVGIEDFAVILPDDLIYNHDC
GTGTLKQMVKAVEGTDIRGCIATQQVKREKTNSYGIVAKDNDNLIKAIVEKPAPEKAPSTNAVVGRYLLP
NKIFRCLESTSEGAGGEIQLTDAIAKLLDQDEKILSYEFKGTRYDCGSKLGFLIANYEIALQHQELGHKF
KEYLQNR

Molecule Role : Virmugen
Molecule Role Annotation : A galU mutant is attenuated in mice, with only transient weight loss observed. Two months after inoculation, mice were challenge with wild type F. tularensis and were protected, once again only experiencing transient weight loss (Jayakar et al., 2011).
Related Vaccine(s): Francisella tularensis galU mutant vaccine

10. groEL

Gene Name : groEL
Sequence Strain (Species/Organism) : Bacillus anthracis
NCBI Protein GI : KHA39277
Taxonomy ID : 1392
Protein Name : molecular chaperone GroEL
Protein pI : 4.52
Protein Weight : 51999.08
Protein Length : 610
Protein Note : 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth; Derived by automated computational analysis using gene prediction method: Protein Homology.

Protein Sequence : Show Sequence

>KHA39277.1 molecular chaperone GroEL [Bacillus anthracis]
MAKDIKFSEEARRSMLRGVDTLANAVKVTLGPKGRNVVLEKKFGSPLITNDGVTIAKEIELEDAFENMGA
KLVAEVASKTNDVAGDGTTTATVLAQAMIREGLKNVTAGANPMGLRKGIEKAVVAAVEELKTISKPIEGK
SSIAQVAAISAADEEVGQLIAEAMERVGNDGVITLEESKGFTTELDVVEGMQFDRGYASPYMITDSDKME
AVLDNPYILITDKKISNIQEILPVLEQVVQQGKPLLIIAEDVEGEALATLVVNKLRGTFNVVAVKAPGFG
DRRKAMLEDIAILTGGEVITEELGRDLKSATVESLGRAGKVVVTKENTTVVEGVGSTEQIEARIGQIRAQ
LEETTSEFDREKLQERLAKLAGGVAVIKVGAATETELKERKLRIEDALNSTRAAVEEGIVAGGGTSLMNV
YTKVASIVAEGDEATGINIVLRALEEPVRQIAINAGLEGSVVVERLKGEKVGVGFNAATGEWVNMLETGI
VDPAKVTRSALQNAASVAAMFLTTEAVVADKPEPNAPAMPDMGGMGGMGGMM

Molecule Role : Protective antigen
Molecule Role Annotation : (Sinha and Bhatnagar, 2013)

11. groEL

Gene Name : groEL
Sequence Strain (Species/Organism) : Francisella tularensis
VO ID : VO_0011075
NCBI Protein GI : 282159959
Other Database IDs : CDD:234573
CDD:239460
Taxonomy ID : 510831
Gene Strand (Orientation) : ?
Protein Name : chaperonin GroEL
Protein pI : 4.6
Protein Weight : 53514.52
Protein Length : 634
Protein Note : chaperonin GroEL; Reviewed; PRK00013

Protein Sequence : Show Sequence

>ADA79350.1 chaperonin GroEL [Francisella tularensis subsp. tularensis NE061598]
MAAKQVLFSDEARAKMLDGVNTLANAVKVTLGPKGRNVVLDKSFGTPTITKDGVSVAKEIELEDKFENMG
AQIVKEVASKTADVAGDGTTTATVLAQALLTEGLKAVAAGMNPMDLKRGIDKATARLVEELKALSKPCSD
PKSIEQVGTISANSDATVGKLIADAMAKVGKEGVITVEEGKGFEDELDVVEGMQFDRGYLSPYFATNQEN
MTTDLENPYILIVDKKISNIRDLLPILEGVSKSGRALLIIAEDVESEALATLVVNNMRGVVKVCAVKAPG
FGDRRKAMLEDIATLTGATFVSEDLSMKLEETNMEHLGTASRVQVTKDNTTIIDGAGEKEAIAKRINVIK
ANIAEANSDYDREKLQERLAKLSGGVAVIKVGAVTEAEMKEKKDRVDDALHATRAAVEEGIVAGGGVALI
RAQKALDGLTGENDDQNYGIALLRKAIEAPLRQIVSNAGGESSVVVNQVKANQGNYGYNAANDTYGDMVE
MGILDPTKVTRSALQHAASIAGLMITTEAMIGEIKEAAPAMPMGGGMGGMPGMM

Molecule Role : Protective antigen
Molecule Role Annotation : Researchers have shown that mice that had been immunized with purified heat shock protein 60 (Hsp60, groEL) isolated from Francisella tularensis were protected against a subsequent challenge with some strains of the bacterium (Hartley et al., 2004).
Related Vaccine(s): F. tularensis GroEL protein vaccine

12. guaA

Gene Name : guaA
Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica LVS
NCBI Gene ID : 3951701
NCBI Protein GI : 89256406
Locus Tag : FTL_1071
Genbank Accession : AM233362
Protein Accession : YP_513768
Taxonomy ID : 376619
Gene Starting Position : 1023478
Gene Ending Position : 1025028
Gene Strand (Orientation) : ?
Protein Name : GMP synthase
Protein pI : 6.04
Protein Weight : 52837.22
Protein Length : 516
Protein Note : contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway

DNA Sequence : Show Sequence

>gi|89255449:1023478-1025028 Francisella tularensis subsp. holarctica LVS chromosome, complete genome
AATGACAGATATACATAATCATAAGATTTTGATTTTAGATTTTGGCTCGCAATATACACAACTTATTGCT
CGTAGAGTAAGAGAAGTAGGTGTTTTTTGTGAAATTTTCCCTCATGATGTAGCAGCTGATTTTATCAAAA
ATTATCAAGCAAAAGGAATAATTTTATCAGGTGGTCCTGAGTCAGTTTATGATTCTGATGTCAAAGCTCC
TGAGATAGTTTTTGAGCTTGGAGTACCTGTTTTGGGTATTTGTTATGGCATGCAAACAATGGTAATGCAG
CATGGTGGCGAAGTAAAAGGAGCTGATCAAAGTGAATTTGGTAAGGCAATTATAAATATCCTAAATTTAA
CAAATAACATATTTTCAAATATGGAGCATGAGCAGCTAGTTTGGATGAGTCATAGTGACAAAGTCACTCA
AACTGGTGAGCATTTTGAGATAATTGCTTCTTCAACAAATGCTCCAGTTGCGGCAGTTGCACATAAAAAT
AAGCCTTTTTTTGGTGTGCAGTTTCACCCAGAAACTACTCATACAGAAAATGGTAAGCAAATTATTGAGA
ATTTTGTCGTTAATATCTGCGGATGTGATACTCTGTGGAATATCGAAAACATTATCGAAAATGATATAAA
AGAAATTAAACAAAAAGTTGGTACTGATAAAGTAATTTTAGGGCTATCTGGAGGTGTTGATTCATCTGTT
GTAGCTGCGATATTACATCAAGCTATCGGCGATCAGCTAACTTGTATATTTGTAGATACTGGTTTACTGC
GCCTTAATGAGGGTGATCAAGTTATGCAAGTATTTGCAGAGCATATGGATATTAATGTTATTCGTATCAA
TGCAAAAAATAGATTCTTAGATGCCCTAAGGGGTATCTGTGATCCAGAACAAAAACGTAAAATTATCGGT
AAGCTATTTGTTGATATTTTTGATGAAGAGGCTGCTAAGATAGAGAATGCTAAATGGCTTGCGCAGGGCA
CTATCTACAGTGATGTAATTGAATCAGCTAGCAATAATCAGTCTAAAGCACATGTAATTAAATCACATCA
TAATGTTGGTGGCTTACCAAAAGAAATGAAACTTAAGCTTTTAGAACCATTAAGAGAGCTTTTCAAAGAT
GAGGTTCGTAAATTAGGCTTAGGTTTAGGATTACCTTATAATATGCTTTATAGACATCCATTTCCTGGTC
CTGGTCTTGGAGTACGTATATTAGGTGAAATAAAGAAAGAGTATGTAGAAACGTTACAAAAAGCTGATGC
TATATTTACAGAAGAGCTTTATAAACATAATTTATACCATGATGTTTCTCAAGCTTTTGGGGTGTTTTTA
CCTATCAAATCGGTAGGTGTAGTTGGTGATCAGCGTAGATATGAGTATGTAATAGCTCTAAGAGCTGTCG
TTAGTATCGATTTTATGACTGCAACGTGGGCTAATTTACCTTATGATTTTCTGTCACTAGTTTCAAATAG
AATTGTAAATGAAGTAAAACAAGTATCAAGAGTTGTGTATGATGTGACAGGAAAACCACCTGGAACAATT
GAATGGGAATA

Protein Sequence : Show Sequence

>gi|89256406|ref|YP_513768.1| GMP synthase [Francisella tularensis subsp. holarctica LVS]
MTDIHNHKILILDFGSQYTQLIARRVREVGVFCEIFPHDVAADFIKNYQAKGIILSGGPESVYDSDVKAP
EIVFELGVPVLGICYGMQTMVMQHGGEVKGADQSEFGKAIINILNLTNNIFSNMEHEQLVWMSHSDKVTQ
TGEHFEIIASSTNAPVAAVAHKNKPFFGVQFHPETTHTENGKQIIENFVVNICGCDTLWNIENIIENDIK
EIKQKVGTDKVILGLSGGVDSSVVAAILHQAIGDQLTCIFVDTGLLRLNEGDQVMQVFAEHMDINVIRIN
AKNRFLDALRGICDPEQKRKIIGKLFVDIFDEEAAKIENAKWLAQGTIYSDVIESASNNQSKAHVIKSHH
NVGGLPKEMKLKLLEPLRELFKDEVRKLGLGLGLPYNMLYRHPFPGPGLGVRILGEIKKEYVETLQKADA
IFTEELYKHNLYHDVSQAFGVFLPIKSVGVVGDQRRYEYVIALRAVVSIDFMTATWANLPYDFLSLVSNR
IVNEVKQVSRVVYDVTGKPPGTIEWE

Molecule Role : Virmugen
Molecule Role Annotation : A guaA mutant is highly attenuated in mice and induces significant protection from challenge with parent strain F. tularensis LVS (Santiago et al., 2009).
Related Vaccine(s): Francisella tularensis guaA mutant vaccine

13. guaB

Gene Name : guaB
Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica FTNF002-00
NCBI Gene ID : 5523917
NCBI Protein GI : 156502929
Locus Tag : FTA_1563
Genbank Accession : CP000803
Protein Accession : YP_001428994
Taxonomy ID : 458234
Gene Starting Position : 1398915
Gene Ending Position : 1400375
Gene Strand (Orientation) : -
Protein Name : inosine-5'-monophosphate dehydrogenase
Protein pI : 7.79
Protein Weight : 47330.3
Protein Length : 486
Protein Note : 1.1.1.205

DNA Sequence : Show Sequence

>gi|156501369:1398915-1400375 Francisella tularensis subsp. holarctica FTNF002-00 chromosome, complete genome
TTTAAGACTGGTAATTAGGTGGTTCTTTAGTAATCGTGACGTTATGTACATGCGATTCATTAAATCCAGC
ACCTGTAATTTGTACAAATGTCGGTTCTGTTCGCATTGTTTGGATATCTTTTGAGCCAGTATAGCCCATA
CTAGATTTCAGACCACCTATTAGCTGATGAATCACTGCAGATAATAAACCTTTGTATGGTACTCTGCCTT
CAACGCCTTCTGGAACAAACTTTTTCGCCTCTGTTTCACTTTGGAAATATCTATCAGATGAGCCTTTCTC
CATAGCACCAAGAGAACCCATACCACGATATGATTTATAAGAGCGACCTTGGAAAAGCTCAACCTCCCCT
GGTGATTCTTCAGTACCTGCAAAAAGCCCTCCAATCATAACTACAGATGCTCCAGCAACTATAGCTTTTG
CAATATCACCGGAATATCTAATACCGCCATCTGCAATCACAGGCACTCCAGTTCCTTTTAATGCCTCTGC
AACATTAGCTATCGCTGTAATTTGTGGTACACCAACACCAGCAACAATTCTGGTAGTACAGATTGAGCCA
GGTCCAATACCAACCTTGACAGCATCAGCACCAGCTTTAACTAGATCTTTAGCAGCTTCTGCTGTAGCGA
TATTACCACCTATAACCTGAATATGTGGATAGTTATCTTTAACCCACTTAACTGTATCTAACACACCTTG
TGAATGTCCATGAGCAGTATCTACAACAATAATATCGACTCCTTCAGCTGCCAATGCTGCAACACGCTCT
TTAGTATTAACAGCGGTTCCAACAGCAGCGCCAACTCTCAAACGCCCAAGAGAATCTTTACAAGCATTTG
GTTTATTTTGTGATCTTTCGATATCTTTTGTTGTAATAAGACCGACCAACTCGCCTTGTTCATTAACTAC
AAGCAACTTCTCAATTTTATGCTCATGAAGTTTCTTTTTAATCGCTCCTTGAGAAGCATCTTCAGGCACT
GTTACAAGTTTTTCTCTAGGTGTCATAATAGAGCTAACAGGTTCATCTAAATCTTTTGCAAATCTAAAAT
CACGCCTTGTAACAATACCTATGATTTTATTATTATCATCAACAACGGGAAAACCAGAGAAATTATGTTC
TTTAGCTAGTTGCATGATTTCTTTAATTGAGCTTTCTTGTTTAATGGTAATGGGATCAATCACCATTCCA
TTTTCAAATCTTTTTACCTTTTTAACTTCTTGGGCTTGGGCTTGAATAGACATATTCTTATGAATAATAC
CAATACCACCTTCCTGGGCTATCGCAATAGCTAGGCGTGACTCAGTAACCGTATCCATAGCTGCAGATAC
TAAAGGAATATTTAACTGAATATCTCTAGTTATATTTGTCTTCAAATCAACTTGATGAGGAAGAACATTT
GAATATCTAGGTGATAGCAATACATCATCAAAAGTGATTGCCTGCTGAGTAATTCTTAACA

Protein Sequence : Show Sequence

>gi|156502929|ref|YP_001428994.1| inosine-5'-monophosphate dehydrogenase [Francisella tularensis subsp. holarctica FTNF002-00]
MLRITQQAITFDDVLLSPRYSNVLPHQVDLKTNITRDIQLNIPLVSAAMDTVTESRLAIAIAQEGGIGII
HKNMSIQAQAQEVKKVKRFENGMVIDPITIKQESSIKEIMQLAKEHNFSGFPVVDDNNKIIGIVTRRDFR
FAKDLDEPVSSIMTPREKLVTVPEDASQGAIKKKLHEHKIEKLLVVNEQGELVGLITTKDIERSQNKPNA
CKDSLGRLRVGAAVGTAVNTKERVAALAAEGVDIIVVDTAHGHSQGVLDTVKWVKDNYPHIQVIGGNIAT
AEAAKDLVKAGADAVKVGIGPGSICTTRIVAGVGVPQITAIANVAEALKGTGVPVIADGGIRYSGDIAKA
IVAGASVVMIGGLFAGTEESPGEVELFQGRSYKSYRGMGSLGAMEKGSSDRYFQSETEAKKFVPEGVEGR
VPYKGLLSAVIHQLIGGLKSSMGYTGSKDIQTMRTEPTFVQITGAGFNESHVHNVTITKEPPNYQS

Molecule Role : Virmugen
Molecule Role Annotation : A guaB mutant is attenuated in mice and induces significant protection from challenge with parent strain F. tularensis LVS (Santiago et al., 2009).
Related Vaccine(s): Francisella tularensis guaB mutant vaccine

14. IglB

Gene Name : IglB
Sequence Strain (Species/Organism) : Francisella novicida U112
VO ID : VO_0011071
NCBI Gene ID : 4548125
NCBI Protein GI : 118497903
Locus Tag : FTN_1323
Genbank Accession : CP000439
Protein Accession : YP_898953
Taxonomy ID : 401614
Gene Starting Position : 1397634
Gene Ending Position : 1399154
Gene Strand (Orientation) : -
Protein Name : intracellular growth locus protein B
Protein pI : 4.5
Protein Weight : 54019.41
Protein Length : 506

DNA Sequence : Show Sequence

>gi|118496615:1397634-1399154 Francisella tularensis subsp. novicida U112, complete genome
TTTAGTTATTATTTGTACCGAATAATTCTGGTTCAAGCCTAGTATCTATAGTCATTGTAGTATTCATTCC
TTCAAATTGAATGTGGGGAATTACATTTATTTTGCATGAATACCAGCCAGGCTTACCCGGTATGGTTTTA
ACCTCGATAGAAACGTTTCTGAAAGGATATCTCGCCATTTCTAAAGGGGTTGGTTGATAGACTGTGGTGA
CAAATTCTGATATCCAATCAGAAAGAATTTTTTGAATCGACTCGACATCCACAATACTACCAATCTTATC
TCTAATTACACATTTAATATAATGAGATATTCTCGATATACACATAGTGTAAGAAAGGTTAGCAATTAAT
CTTGAGTTTGCTGAGTCAAATCCATCTACAAATTCTTCGACTTTTTTAGCAGAATTTACACTAAAGAAAC
AAGCGTTACTAGTACCTTTTTCACTTACAAATGGTATTAAACCAATATTTGCAAGTGATAACTCCATATA
ATCAGCAAATAATACATTTAGAGGTGACTTAGTTTCTAGAATGCCTTTATTATCATATACGCAAGCTACC
AAGTTTTTTACATAACCACCACTCTCAACTCCTCTTATATATTGGAACCATCTAGTTTTATCATAAGATC
TCATCATATTCTTAACTAGATGAATTGAAGCAGGTCCCCATAGATAACTTTCATTCTTATCATAATCAAC
AAACTCATTAAAGCCTTCCATAAGTTTATACTGAACTGGATTATTCTCAGGATTATATGGTTGCCGCAAC
ATAAAATCACCTACGGTCAAACCTATATATGCAGCAACATCAAGGTTTCTAAAATCATTCCACTCTTTAT
ATCTAGGATGCTCAAGCAAAGCTTCAAAACTTTTTATATGAGTGATTTCTGATAAATCCTTAACACCAAA
GAATGACTTATCAATTGATGCAATAAATGGTGCATGAGAATTCTTTGCCACCATACCCATTCCAGTCAAC
CAAATTATATCATTTGTGGTATTTTCAAAATTATACAATCCTAATATTGCGCCATAAGGTTCGCCACCAT
ATTGATCAAATTCTGAAACATATACTTTTTTGAAAAAGTCACTACTAGATATATCATATAAATTTCTCTC
GAAATCATATTGTAGCTCTTCTTTTTTTACATCTAATATACTTACTTCAACATTATCATAGTCTTCTTGA
CAAACCTCTTGCACCTTTAGCCATTCTTGCTCAAGTGCTCTAAACTCATCATTAGATATAATAGAGTTAA
CTTGTAAATCAATCAGCTTATCAATAACTGTAATAACTTTTTGAATATATTTTTGATTGTAATTATTTAT
ATTATCATTATTTGCTAATACCAAAGATAGCGCCATAAGGTTTCTAGCATTGTAGTCAGTAGATAAAGAT
AAAACTTTAGAAGCATCATCTGAAACATCAAAATCTATATTTTTGAGTACACTATCAACTTCTGTAGATC
CCCCAAAATTATTTAAAAGTTCATCAGTGAGACTTAATTTATTTATTGTCA

Protein Sequence : Show Sequence

>gi|118497903|ref|YP_898953.1| intracellular growth locus protein B [Francisella tularensis subsp. novicida U112]
MTINKLSLTDELLNNFGGSTEVDSVLKNIDFDVSDDASKVLSLSTDYNARNLMALSLVLANNDNINNYNQ
KYIQKVITVIDKLIDLQVNSIISNDEFRALEQEWLKVQEVCQEDYDNVEVSILDVKKEELQYDFERNLYD
ISSSDFFKKVYVSEFDQYGGEPYGAILGLYNFENTTNDIIWLTGMGMVAKNSHAPFIASIDKSFFGVKDL
SEITHIKSFEALLEHPRYKEWNDFRNLDVAAYIGLTVGDFMLRQPYNPENNPVQYKLMEGFNEFVDYDKN
ESYLWGPASIHLVKNMMRSYDKTRWFQYIRGVESGGYVKNLVACVYDNKGILETKSPLNVLFADYMELSL
ANIGLIPFVSEKGTSNACFFSVNSAKKVEEFVDGFDSANSRLIANLSYTMCISRISHYIKCVIRDKIGSI
VDVESIQKILSDWISEFVTTVYQPTPLEMARYPFRNVSIEVKTIPGKPGWYSCKINVIPHIQFEGMNTTM
TIDTRLEPELFGTNNN

Molecule Role : Virmugen
Molecule Role Annotation : Intranasal vaccination with KKF235 (F. tularensis subsp. novicida mutant (DeltaiglB) ) protected mice from subsequent homotypic challenge with U112 as well as heterotypic challenge with F. tularensis subsp. holarctica (LVS). Most significantly, oral immunization with KKF235 protected mice from a highly lethal subsp. tularensis (SCHU S4) pulmonary challenge (Yu et al., 2010).
Related Vaccine(s): Francisella tularensis IglB mutant vaccine

15. IglC

Gene Name : IglC
Sequence Strain (Species/Organism) : Francisella tularensis subsp. novicida U112
NCBI Gene ID : 4548124
Locus Tag : FTN_1322
Gene Strand (Orientation) : ?

DNA Sequence : Show Sequence

>gi|118496615:1396989-1397618 Francisella tularensis subsp. novicida U112, complete genome
CTATGCAGCTGCAATATATCCTATTTTAGCAACTCCTTTATCTGTGCTAGCTGCGCAACATACTGGCAAG
CTTCCTTGAGATGTTGTAAGACCATCTATAGATATAATATGATAACTCATTCCATCTTTTGAAACACCCA
TAAGTTCTGTTGGCTCTATACTAATACTAAAAGCCTTAGCACTTATTGGATATAACTCTAAATTAGATAG
ATCTATCATAATACCCCATGCTTCATCAGTTTTGTACTCTTGTTTTTTATTAGAATTACCTAACTTAATT
TTCATATCTGTAGCACTTGCTTGTAATATGCTCGAAACTTTCTCTTCAATTTTAGTTCTGACATTTGATT
TAATTAGAACACCAAGAGTAATACTTATTGTTGGATTTATAGAGCCTGGCGTTATAGATAAACGTATTAC
ACTAGCAGCAGCTGTAGCCGAATCAGCTTTCGTGACATCCTCTCCACCCTCTATAGCAACGATATTTTTA
TCAAGTTTCTCCCCAGCTATAGTTAAAGAATCAATAAATAATCTACAATTAGGATGAGATCCTATACATG
CAGTAGGATCAGTTCTCACATGAATGGTCTCGCCACTTGTTACCTGTTGTCTTGTTATCATCTCACTCAT

Molecule Role : Virmugen
Molecule Role Annotation : An iglC mutant is highly attenuated in mice. Inoculated mice were challenged 30 days later with wild type F. tularensis and were highly protected (Pammit et al., 2006).
Related Vaccine(s): KKF24

16. IglC1

Gene Name : IglC1
Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
VO ID : VO_0011072
NCBI Gene ID : 3191591
NCBI Protein GI : 56708413
Locus Tag : FTT_1357c
Genbank Accession : AJ749949
Protein Accession : YP_170309
3D structure: PDB ID : 2QWU
Taxonomy ID : 177416
Gene Starting Position : 1398637
Gene Ending Position : 1399272
Gene Strand (Orientation) : -
Protein Name : intracellular growth locus subunit C
Protein pI : 6.26
Protein Weight : 21340.81
Protein Length : 211
Protein Note : Similar to AAP58964.1 (Q7X3J5) IglC (23 kDa protein) from Francisella novicida (211 aa). BLAST Score = 405, Expect = e-112 Identities = 209/211 (99), Positives = 210/211 (99) Identical to FTT1712. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625

DNA Sequence : Show Sequence

>NC_006570.2:1398637-1399272 Francisella tularensis subsp. tularensis SCHU S4 chromosome, complete genome
ACTATGCAGCTGCAATATATCCTATTTTAGCAACTCCTTTATCTGTGCTAGCTGCGCAACATACTGGCAA
ACTTCCTTGAGATGTTGTAAGACCATCTATAGATATAATATGATATCTCATTCCATCTTTTGAAACACCC
ATAAGTTCTGTTGGCTCTATACTAATACTAAAAGCCTTAGCACTTATTGGATATAACTCTAAATTAGATA
GATCTATCATAATACCCCATGCTTCATCAGTTTTATACTCTTGTTTTTTATTAGAATTACCTAACTTAAT
TTTCATATCTGTAGCACTTGCTTGTAATATACTCGAAACTTTCTCTTCAATTTTAGTTCTAACATTTGAT
TTAATTAGAACACCAAGAGTAATACTTATTGTTGGATTTATAGAGCCTGGCGTTATAGATAAACGTATTA
CACTAGCAGCAGCTGTAGCCGAATCAGCTTTCGTGACATCCTCTCCACCATCTATAGCAACGATATTTTT
ATCAAGTTTCTCCCCAGCTATAGTTAAAGAATCAATAAATAATCTACAATTAGGATGAGATCCTATACAT
GCAGTAGGATCAGTTCTCACATGAATGGTCTCGCCACTTGTTACCTGTTGTCTTGTTATCATCTCACTCA
TAATCA

Protein Sequence : Show Sequence

>YP_170309.1 intracellular growth locus subunit C [Francisella tularensis subsp. tularensis SCHU S4]
MIMSEMITRQQVTSGETIHVRTDPTACIGSHPNCRLFIDSLTIAGEKLDKNIVAIDGGEDVTKADSATAA
ASVIRLSITPGSINPTISITLGVLIKSNVRTKIEEKVSSILQASATDMKIKLGNSNKKQEYKTDEAWGIM
IDLSNLELYPISAKAFSISIEPTELMGVSKDGMRYHIISIDGLTTSQGSLPVCCAASTDKGVAKIGYIAA
A

Molecule Role : Protective antigen
Molecule Role Annotation : Mice immunized with rLm/iglC were protected against lethal challenge with F. tularensis LVS administered by the intranasal route, a route chosen to mimic airborne infection, and, most importantly, against aerosol challenge with the highly virulent Type A F. tularensis SchuS4 strain (Jia et al., 2009).
Related Vaccine(s): F. tularensis vaccine rLm/iglC

17. IglC2

Gene Name : IglC2
Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
VO ID : VO_0011073
NCBI Gene ID : 3191814
NCBI Protein GI : 56708721
Locus Tag : FTT_1712c
Genbank Accession : AJ749949
Protein Accession : YP_170617
Taxonomy ID : 177416
Gene Starting Position : 1791972
Gene Ending Position : 1792607
Gene Strand (Orientation) : -
Protein Name : intracellular growth locus subunit C
Protein pI : 6.26
Protein Weight : 21340.81
Protein Length : 211
Protein Note : Similar to AAP58964.1 (Q7X3J5) IglC from Francisella novicida (23 kDa protein) (211 aa). BLAST Score = 405,Expect = e-112 Identities = 209/211 (99), Positives = 210/211 (99). Identical to FTT1357. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281

DNA Sequence : Show Sequence

>NC_006570.2:1791972-1792607 Francisella tularensis subsp. tularensis SCHU S4 chromosome, complete genome
ACTATGCAGCTGCAATATATCCTATTTTAGCAACTCCTTTATCTGTGCTAGCTGCGCAACATACTGGCAA
ACTTCCTTGAGATGTTGTAAGACCATCTATAGATATAATATGATATCTCATTCCATCTTTTGAAACACCC
ATAAGTTCTGTTGGCTCTATACTAATACTAAAAGCCTTAGCACTTATTGGATATAACTCTAAATTAGATA
GATCTATCATAATACCCCATGCTTCATCAGTTTTATACTCTTGTTTTTTATTAGAATTACCTAACTTAAT
TTTCATATCTGTAGCACTTGCTTGTAATATACTCGAAACTTTCTCTTCAATTTTAGTTCTAACATTTGAT
TTAATTAGAACACCAAGAGTAATACTTATTGTTGGATTTATAGAGCCTGGCGTTATAGATAAACGTATTA
CACTAGCAGCAGCTGTAGCCGAATCAGCTTTCGTGACATCCTCTCCACCATCTATAGCAACGATATTTTT
ATCAAGTTTCTCCCCAGCTATAGTTAAAGAATCAATAAATAATCTACAATTAGGATGAGATCCTATACAT
GCAGTAGGATCAGTTCTCACATGAATGGTCTCGCCACTTGTTACCTGTTGTCTTGTTATCATCTCACTCA
TAATCA

Protein Sequence : Show Sequence

>YP_170617.1 intracellular growth locus subunit C [Francisella tularensis subsp. tularensis SCHU S4]
MIMSEMITRQQVTSGETIHVRTDPTACIGSHPNCRLFIDSLTIAGEKLDKNIVAIDGGEDVTKADSATAA
ASVIRLSITPGSINPTISITLGVLIKSNVRTKIEEKVSSILQASATDMKIKLGNSNKKQEYKTDEAWGIM
IDLSNLELYPISAKAFSISIEPTELMGVSKDGMRYHIISIDGLTTSQGSLPVCCAASTDKGVAKIGYIAA
A

Molecule Role : Protective antigen
Molecule Role Annotation : Mice immunized with rLm/iglC were protected against lethal challenge with F. tularensis LVS administered by the intranasal route, a route chosen to mimic airborne infection, and, most importantly, against aerosol challenge with the highly virulent Type A F. tularensis SchuS4 strain (Jia et al., 2009).
Related Vaccine(s): F. tularensis vaccine rLm/iglC

18. LpnA

Gene Name : LpnA
Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
VO ID : VO_0011079
NCBI Gene ID : 3191792
NCBI Protein GI : 56708002
Locus Tag : FTT_0901
Genbank Accession : AJ749949
Protein Accession : YP_169898
Taxonomy ID : 177416
Gene Starting Position : 909953
Gene Ending Position : 910402
Gene Strand (Orientation) : +
Protein Name : lipoprotein
Protein pI : 9.16
Protein Weight : 14909.46
Protein Length : 149
Protein Note : Paralog to conserved hypothetical lipoprotein lpnB.

DNA Sequence : Show Sequence

>NC_006570.2:909953-910402 Francisella tularensis subsp. tularensis SCHU S4 chromosome, complete genome
TATGAAAAAAATAATTGAGCTTAGTCTTTTATCTTTATCAATCGCAGGTTTAGCGAGCTGTTCTACTCTA
GGGTTAGGTGGCTCTGATGATGCAAAAGCTTCAGCTAAAGATACTGCTGCTGCTCAGACAGCTACTACTG
AGCAAGCTGCTGCTGTATCTAAGCCAACTGCAAAAGTAAGTTTAAATAAACTTGGTCAGGATAAAATAAA
AGCAACTGTATATACAACATACAATAATAACCCACAAGGAAGTGTAAGATTACAATGGCAGGCTCCAGAA
GGTTCTAAGTGCCATGATACAAGCTTCCCAATTACTAAGTATGCTGAGAAGAACGATAAAACTTGGGCAA
CTGTAACAGTTAAGCAAGGTAATAACTTCTGTAGCGGTAAGTGGACAGCTAATGTAGTTTATGACAAAGA
AGTAATCGCTTCTGATTCAATAAATATTTA

Protein Sequence : Show Sequence

>YP_169898.1 lipoprotein [Francisella tularensis subsp. tularensis SCHU S4]
MKKIIELSLLSLSIAGLASCSTLGLGGSDDAKASAKDTAAAQTATTEQAAAVSKPTAKVSLNKLGQDKIK
ATVYTTYNNNPQGSVRLQWQAPEGSKCHDTSFPITKYAEKNDKTWATVTVKQGNNFCSGKWTANVVYDKE
VIASDSINI

Molecule Role : Protective antigen
Molecule Role Annotation : Anti-lpnA MAbs conferred significant, although not complete, protection of naïve C3H/HeN mice from lethal challenge with F. tularensis LVS when administered therapeutically (Savitt et al., 2009).

19. PurC/PurD

Gene Name : PurC/PurD
Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica LVS
NCBI Gene ID : 3952576
NCBI Protein GI : 89255807
Locus Tag : FTL_0396
Genbank Accession : AM233362
Protein Accession : YP_513169
Taxonomy ID : 376619
Gene Starting Position : 365666
Gene Ending Position : 367978
Gene Strand (Orientation) : ?
Protein Name : fusion protein PurC/PurD
Protein pI : 5.81
Protein Weight : 82357.33
Protein Length : 770
Protein Note : 6.3.2.6

DNA Sequence : Show Sequence

>gi|89255449:365666-367978 Francisella tularensis subsp. holarctica LVS chromosome, complete genome
AATGTTTGATAAACAAATCATTGCTAATAATATAAAAAATGTTCTAAAATCAACAAATCTTGATATCAAG
AATAAATATATAGGTAAAGTAAGAGATATGTACTTTACTGATGACAAAAGTATTTTGATATCTACAGATA
GACAATCTGCTTTTGATAGATCTTTGGGTTTTATACCATTTAAGGGGCAAATCCTTGCACAATCATCAGT
ATGGTGGTTCAAAGAAACAGCACATATTGTCAAAAACCACTTTATAGACTCTCCAGATCCAAATGTTGTC
ATCGCGAGAAAGGCAAAAGTATTGCCAATTGAGTTTGTCGTTAGAGGTTATATAACTGGTTCAACTTCTA
CATCATTGTGGACACATTATAAAAACGGTAGCCGTGACTATTGTGGCAATATCCTGCCAGAAGGTTTAAA
AAAGAATCAAAAATTACCACAAAATATCCTTACACCAACTACCAAAGAGCAAGATCATGATCGCCCAATT
TCAGCTGAGGATATTGTCAAGGAAGGTTGGCTAACTCAGCAGCAGTGGGATTTTGCTTCACAAAAGGCTC
TAGAACTTTTTGAGTTTGGACAAAAAAAAGCCTTAGAACATGGCTTATTTTTGGCGGATACTAAATATGA
GTTTGGTATTGATGAGCAAACTGGAGAGATTATTTTGATCGATGAGATCCATACTCCAGATAGCTCAAGA
TTTTGGCTAAAAGATAGCTATGCTACAAGATTTGAAAATGGTGAAGAACCAGAGAATATTGATAAAGAAT
TTTTTAGATTATGGTTTGCAAAAAATTGTGACCCATATAATGATGAAGTTTTACCACAAGCACCACAAGA
GTTAGTTGTCGAATTATCTCAAAAATATATAACCCTTTTTGAAATGATAACAGGGCAAAAGTTTGAAGTA
CCAAGAGATTTAGAAAATATAAATCAACGTATTGTTAAAAATGTTACAGATTACTTAAATATGGAGAAGC
CAGTGAATATTCTTCTAGTAGGATCAGGAAGTAGAGAGCATGCGATAGCAGAAGCAGTCAAAAGAAGCTC
TATTGCAAATAAGCTTTTTTGTATAAGCACAGCGATTAACCCTGCGATAGATAAGATAACGCAAGGGTAT
CAAATCGCTGATATTTGTAATTGTGATGAAGTACTAGAGTATGCAAAATCACAAAGTATTGATATTGCGA
TTATCGGTCCTGAAGCACCACTTGAGGCTGGTCTAGCTGATGCACTTAAGACAGCAGCAATTGGCGTAGT
TGGTCCAACCAAAAAGTTAGCACAGCTTGAAACATCAAAAGGTTTCACTAGAGATCTGATCCGTGATTAT
GATATAGGCGCTAATCCTTTCTTTAGAAAGTTTAACTCGATGGATGGAGTCGAAGAGACTATCAAAAAAT
ATCAAAATCAATTTGTCATAAAAGCTGATGGTTTATGTGGTGGTAAAGGAGTGCTTGTTTGGGGCGATCA
TTTGCACAGCCTTGATGAGGCGATTAGACACTGTCAGTCATTGGTAGATGCTGGCAAAGAGTTTGTTATC
GAGGAGAAGCTTGTTGGCCAGGAGTTCTCGCTGATATCATTTACTGATGGCAAAAATTTTATCCATATGC
CAGCAGTCCAAGATCATAAGCGTGCCCATGAAGGTGACAAAGGTCCTAATACCGGTGGTATGGGTACTTA
TTCTGATGCTAATCATAGTTTACCGTTTTTATCAGCTGCTGATATCGAAAGAGCAAAACAAATAAATGAG
AAAGTTGTCAGAGCTTTAGCTGATAAATTTTGTGAACCATATCAAGGTATCCTTTATGGTGGCTTTATGG
CGACAAAAGATGATACTAAAGTGATCGAGTATAATGCACGTTTTGGTGATCCAGAAGCGATGAATCTACT
TACACTGTTAGAGACAGATTTTGTTGAGATTGCTCAAGCTATTACACAGGGTAAACTTGATACGGTCAAA
GCTAAGTTTAAAAATCAAGCAAGCGTATGTAAATATCTAGTACCGCTTGGCTATCCAAACCAATCAGTTA
AAAACTTTGAGATAGATATTTCACAATGCCCTGATAATGTCGAGCTTTTCTTAGGTGCTGTTGATTATAA
AGATGGTAAGCTAATTGGTACAGGTTCTAGAGCTATAGCTGTACTTGGTTTAGGTGATACAATAGCTGAA
GCAGAGCAAAAAGCCGAAAATGCTGTCAAAAATATTTATGGTAAACTATTTCATCGTCCAGATATTGGTA
CTAAAGAGCTTATCAATAAGCGTATCAAACACATGAATTTGTTGCGTGGTGATAAATATCAGGAGCTTAA
ATA

Protein Sequence : Show Sequence

>gi|89255807|ref|YP_513169.1| fusion protein PurC/PurD [Francisella tularensis subsp. holarctica LVS]
MFDKQIIANNIKNVLKSTNLDIKNKYIGKVRDMYFTDDKSILISTDRQSAFDRSLGFIPFKGQILAQSSV
WWFKETAHIVKNHFIDSPDPNVVIARKAKVLPIEFVVRGYITGSTSTSLWTHYKNGSRDYCGNILPEGLK
KNQKLPQNILTPTTKEQDHDRPISAEDIVKEGWLTQQQWDFASQKALELFEFGQKKALEHGLFLADTKYE
FGIDEQTGEIILIDEIHTPDSSRFWLKDSYATRFENGEEPENIDKEFFRLWFAKNCDPYNDEVLPQAPQE
LVVELSQKYITLFEMITGQKFEVPRDLENINQRIVKNVTDYLNMEKPVNILLVGSGSREHAIAEAVKRSS
IANKLFCISTAINPAIDKITQGYQIADICNCDEVLEYAKSQSIDIAIIGPEAPLEAGLADALKTAAIGVV
GPTKKLAQLETSKGFTRDLIRDYDIGANPFFRKFNSMDGVEETIKKYQNQFVIKADGLCGGKGVLVWGDH
LHSLDEAIRHCQSLVDAGKEFVIEEKLVGQEFSLISFTDGKNFIHMPAVQDHKRAHEGDKGPNTGGMGTY
SDANHSLPFLSAADIERAKQINEKVVRALADKFCEPYQGILYGGFMATKDDTKVIEYNARFGDPEAMNLL
TLLETDFVEIAQAITQGKLDTVKAKFKNQASVCKYLVPLGYPNQSVKNFEIDISQCPDNVELFLGAVDYK
DGKLIGTGSRAIAVLGLGDTIAEAEQKAENAVKNIYGKLFHRPDIGTKELINKRIKHMNLLRGDKYQELK

Molecule Role : Virmugen
Molecule Role Annotation : A purMCD mutant in Francisela tularensis was attenuated. A low dosage of F. tularensis LVS delta purMCD mutant provided protection against challenge in mice (Pechous et al., 2006).
Related Vaccine(s): Francisella tularensis purMCD mutant vaccine

20. purF

Gene Name : purF
Sequence Strain (Species/Organism) : Francisella novicida U112
NCBI Gene ID : 4548578
NCBI Protein GI : 118498261
Locus Tag : FTN_1700
Genbank Accession : CP000439
Protein Accession : YP_899311
Taxonomy ID : 401614
Gene Starting Position : 1820533
Gene Ending Position : 1822023
Gene Strand (Orientation) : -
Protein Name : amidophosphoribosyltransferase
Protein pI : 7.32
Protein Weight : 51173.39
Protein Length : 496
Protein Note : Catalyzes first step of the de novo purine nucleotide biosynthetic pathway

DNA Sequence : Show Sequence

>gi|118496615:1820533-1822023 Francisella tularensis subsp. novicida U112, complete genome
ATTAGTTATCAAACCCTTTGTATTTTTTTTCTAATTCTTTTAACTCTTTACGATGTTTCTCTAGCGCATC
TAGATAAGCATCATCGACATCACCTGTAATGTAATTACCAGAGAAAACACTATCCTCAAACTCTCTAATT
TTAGGGTTTTGCTTTTGAATAATCTCTTTTAGATCCTCTAATGGTAAATATATTAGTCCATCTACTCCAA
TCCACTGGCGAATCTCTTCTATAGTCTTTCCATGAGCAATTAAATCCGACTTAACCGGCATATCAATACC
ATAAACATTTGGATAACGAACGGCTGGAGATACTGAAGCAAGATAAACAGATTTAGCACCTAAATCTCTA
ATCATCTCAATAATCCGCTTAGATGTTGTACCTCGGACAATAGAATCATCAACAAGTAGAACATTCTTAT
CTCTAAACTCTGCTGGAATAGGGTTTAGTTTTCTTCTAACAAAATTCTTTCTATCAACATTCTCTGGCAT
GATAAAAGTTCTACCAACATAGCGATTCTTAACAAAACCTTCACGATACTCAACACCTAATGCTGTTGCA
ATCTCTTGAGCAGAAGCTCTACCTGTCTCAGGTACAGGGATAACGATATCGATATCCTTATCTTTCCACG
CTTCTTTGATTCTCTTACTTAGAATTTTACCAGCATCTACTCGCGCTTGATAAACACTCACACCATTCAT
AATACTATCAGGACGTGCAAAGTATACATACTCAAATAGACACGGTGCCAAAACTGGATTTTTTGCACAG
ATTTTAGAGTGAACCTTTCTATCCTCTGTAATTATTATCACCTCACCAGGTTCAACATCACGTAATACTT
TAAATCCGGAAATATCTAAAGCAACACTTTCACTAGCAACCATGTAAGCTTTTTCACCATCATCGTACTC
TTTAAAACCAAGCACAAGTGGACGAATACCATAAGGATCTCTAAAAGCAATTAAACCAAAATTTGCTATC
ATCGCAGTACAAGCATAGCCCCCTTTGACATGTTCAAAAACAAATTTACATGCTTTATAAACAGCCTCAG
AAGTCGCAGATCCTTTTGATTTATTCATCCCACAAGCAAAGAAATTAAGCAATAATTCGGAATCTGAACT
TGTATTAAGATGGCGTCTTTCAATATCATGGAGCATTTGTGCAAGCTCTGGCACATTAGTGAGATTGCCA
TTATGAGCAAAGACTATACCATGAGGATTATTTACATAAAAAGGTTGGCTATCAGCAGCACCCAAGCTAC
CTGCTGTAGGATATCTAACATGACCGATACCCATATTACCTTTTGACTTCTCTAGTTTTTCATCTGTAAA
TACATCACTTACTAGACCTGTATTTTTACGGATAAAAAAGTGCCCACGATCCATAGTTGCAATCCCTGCA
GCATCTTGACCTCTATGCTGCAAAAGACTTAAACCATAAAATAATGCATAACTAACCTGATCTGGTCCAG
CGACTCCAATTACTCCACACA

Protein Sequence : Show Sequence

>gi|118498261|ref|YP_899311.1| amidophosphoribosyltransferase [Francisella tularensis subsp. novicida U112]
MCGVIGVAGPDQVSYALFYGLSLLQHRGQDAAGIATMDRGHFFIRKNTGLVSDVFTDEKLEKSKGNMGIG
HVRYPTAGSLGAADSQPFYVNNPHGIVFAHNGNLTNVPELAQMLHDIERRHLNTSSDSELLLNFFACGMN
KSKGSATSEAVYKACKFVFEHVKGGYACTAMIANFGLIAFRDPYGIRPLVLGFKEYDDGEKAYMVASESV
ALDISGFKVLRDVEPGEVIIITEDRKVHSKICAKNPVLAPCLFEYVYFARPDSIMNGVSVYQARVDAGKI
LSKRIKEAWKDKDIDIVIPVPETGRASAQEIATALGVEYREGFVKNRYVGRTFIMPENVDRKNFVRRKLN
PIPAEFRDKNVLLVDDSIVRGTTSKRIIEMIRDLGAKSVYLASVSPAVRYPNVYGIDMPVKSDLIAHGKT
IEEIRQWIGVDGLIYLPLEDLKEIIQKQNPKIREFEDSVFSGNYITGDVDDAYLDALEKHRKELKELEKK
YKGFDN

Molecule Role : Virmugen
Molecule Role Annotation : Mice, which had been immunized with at least 6.95×103 cfu of F. tularensis U112 purF::Cm, were protected against
the subsequent F. tularensis subspecies novicida challenge (Quarry et al., 2007).
Related Vaccine(s): Francisella tularensis purF mutant vaccine

21. purM

Gene Name : purM
Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica LVS
NCBI Gene ID : 3952575
NCBI Protein GI : 89255806
Locus Tag : FTL_0395
Genbank Accession : AM233362
Protein Accession : YP_513168
Taxonomy ID : 376619
Gene Starting Position : 364623
Gene Ending Position : 365666
Gene Strand (Orientation) : ?
Protein Name : phosphoribosylaminoimidazole synthetase
Protein pI : 5.23
Protein Weight : 35240.61
Protein Length : 347
Protein Note : catalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis

DNA Sequence : Show Sequence

>gi|89255449:364623-365666 Francisella tularensis subsp. holarctica LVS chromosome, complete genome
AATGGCAGGCTTAAAATATGAAGATGCTGGCGTAAATATCGAAGCTGGTAATCAAGCTGTAGAAAGAATG
AAGCAGCATGTCAAAAAAACTTTTACCCAAGATGTTTTGACAGGTTTGGGTAGCTTTGGTTCTTTATACT
CACTAAAAAATATTATAAATAATTATGATGATCCTGTCTTAGTACAGTCGATTGATGGTGTTGGTACAAA
GACAAAAGTCGCGGTAATGTGTGGTAAGTTTGAAAACCTTGGTTATGATCTTTTCTCAGCTGCGACAAAT
GATATTGTTGTAATGGGTGCCAAGCCAATTACGTTTCTAGATTATGTCGCGCATGATAAGCTTGATCCTG
CAATTATGGAAGAGCTCGTCAAAGGGATGTCAAAAGCTTGTGCTGAGTGTGGGGTTTCGCTGGTAGGTGG
CGAGACTGCGGAAATGCCTGGAGTATATCAAGCTGGTGAGATTGACATGGTTGGAGTTATCACTGGTATC
GTTGGTAGAAAAAGAATTATCAATGGTGAGAATATCAAAGAGGGTGATATTGTCTTTGGGCTTAGTTCAT
CAGGATTACATACAAATGGCTACTCTTTTGCACGTAAACTATTTTTTGATGTAGCAGGCAACAAACATAC
TGATACTTATCCAGAGTTAGAGGGTAAAACTATCGGTGATGTGCTACTTGAACCACATATCAACTATACT
AATATCATCCATGATTTCTTAGATAATGGTGTTGATATCAAAGGTATGGCACATATCACAGGTGGCGGGT
TTATCGAGAATATTCCACGCGTATTACCACAAGGTTTAGGTGCCCAAATTGACAAAGATAGTTTTGCAAC
TCCAGCTATTTTTAAGCTAATGCAGAGAATCGGCGATATAAGCGAGTTTGAAATGTATCGCTCATTTAAT
ATGGGTATTGGTATGACGATAATTGCTAGCCAAGATCAATTTGACAAGATGCAAGAATTAGCTAAAAAAC
ATACTAATACAAAACTATATCAAATTGGTAAAATCACAAATTCAGGAAAGGTAGAAATTATCTA

Protein Sequence : Show Sequence

>gi|89255806|ref|YP_513168.1| phosphoribosylaminoimidazole synthetase [Francisella tularensis subsp. holarctica LVS]
MAGLKYEDAGVNIEAGNQAVERMKQHVKKTFTQDVLTGLGSFGSLYSLKNIINNYDDPVLVQSIDGVGTK
TKVAVMCGKFENLGYDLFSAATNDIVVMGAKPITFLDYVAHDKLDPAIMEELVKGMSKACAECGVSLVGG
ETAEMPGVYQAGEIDMVGVITGIVGRKRIINGENIKEGDIVFGLSSSGLHTNGYSFARKLFFDVAGNKHT
DTYPELEGKTIGDVLLEPHINYTNIIHDFLDNGVDIKGMAHITGGGFIENIPRVLPQGLGAQIDKDSFAT
PAIFKLMQRIGDISEFEMYRSFNMGIGMTIIASQDQFDKMQELAKKHTNTKLYQIGKITNSGKVEII

Molecule Role : Virmugen
Molecule Role Annotation : A purMCD mutant in Francisella tularensis was attenuated. A low dose of the F. tularensis LVS delta purMCD mutant provided protection against subsequent lethal challenge with the LVS parental strain (Pechous et al., 2006).
Related Vaccine(s): Francisella tularensis purMCD mutant vaccine

22. SodB from F. tularensis SCHU S4

Gene Name : SodB from F. tularensis SCHU S4
Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
VO ID : VO_0011077
NCBI Gene ID : 3190713
NCBI Protein GI : 56707247
Locus Tag : FTT_0068
Genbank Accession : AJ749949
Protein Accession : YP_169143
3D structure: PDB ID : 3H1S
Taxonomy ID : 177416
Gene Starting Position : 70160
Gene Ending Position : 70738
Gene Strand (Orientation) : ?
Protein Name : superoxide dismutase (Fe)
Protein pI : 5.29
Protein Weight : 20748.47
Protein Length : 192
Protein Note : Similar to SODF_ECOLI (P09157) Superoxide dismutase [Fe] from E. coli (192 aa). FASTA: opt: 896 Z-score: 1143.2 E(): 8.8e-56 Smith-Waterman score: 896; 66.316 identity in 190 aa overlap

DNA Sequence : Show Sequence

>gi|255961454:70160-70738 Francisella tularensis subsp. tularensis SCHU S4, complete genome
AATGAAATTTGAATTACCAAAACTACCTTACGCTGTTGATGCATTAGAGTCAACAATATCAAAAGAAACA
ATAGAGTATCACTATGGTAAACATCATCAAACATATGTAACTAATCTAAATAATTTAGTTGAGGGTACAG
AGCACGATGGCAGAAACCTAGAAGAAATCGTAAAAACTTCTAATGGCGGAATATTTAATAACGCTGCTCA
AGTTTTTAATCATACTTTTTACTGGAATTGTTTAACTCCAAACAAAACAGAAGCTTCAAGTCAGTTAAAA
GCAGCATTGATCGAGACATTTGGTTCTGTAGAAAATTTTAAAGAACAATTCTCTAAGGCAGCTATTGCAA
CATTTGGTTCTGGTTGGGCTTGGTTAGTAAAAAATACTGAAGGTAAACTTGAAATAGTAACTACAAGTAA
CGCTGGTTGCCCATTAACAGAGAACAAAAAGCCATTGCTAACTTTTGATGTTTGGGAGCACGCATACTAT
ATTGATTATCGTAATGCTAGACCTAAATATGTTGAAGCATTATGGGATATCGTAAACTGGCAATTTGTTT
CTGAGCAATTCGCTGATTA

Protein Sequence : Show Sequence

>gi|56707247|ref|YP_169143.1| superoxide dismutase (Fe) [Francisella tularensis subsp. tularensis SCHU S4]
MKFELPKLPYAVDALESTISKETIEYHYGKHHQTYVTNLNNLVEGTEHDGRNLEEIVKTSNGGIFNNAAQ
VFNHTFYWNCLTPNKTEASSQLKAALIETFGSVENFKEQFSKAAIATFGSGWAWLVKNTEGKLEIVTTSN
AGCPLTENKKPLLTFDVWEHAYYIDYRNARPKYVEALWDIVNWQFVSEQFAD

Molecule Role : Virmugen
Molecule Role Annotation : C57BL/6 mice were vaccinated intranasally (i.n.) with the sodB(Ft) mutant and challenged i.n. with lethal doses of F. tularensis SchuS4. SodB(Ft) vaccinated mice following SchuS4 challenge exhibited significantly reduced bacterial burden in lungs, liver, and spleen, regulated production of pro-inflammatory cytokines, and less severe histopathological lesions compared to the LVS vaccinated mice. The sodB(Ft) vaccination induced a potent humoral immune response and protection against SchuS4 required both CD4 and CD8 T cells in the vaccinated mice (Bakshi et al., 2008).
Related Vaccine(s): sodB(Ft)

23. sodC

Gene Name : sodC
Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica FTNF002-00
NCBI Gene ID : 5522867
NCBI Protein GI : 156501771
Locus Tag : FTA_0403
Genbank Accession : CP000803
Protein Accession : YP_001427836
Taxonomy ID : 458234
Gene Starting Position : 350894
Gene Ending Position : 351451
Gene Strand (Orientation) : +
Protein Name : copper/zinc superoxide dismutase
Protein pI : 5.61
Protein Weight : 18894.63
Protein Length : 185
Protein Note : 1.15.1.1

DNA Sequence : Show Sequence

>gi|156501369:350894-351451 Francisella tularensis subsp. holarctica FTNF002-00 chromosome, complete genome
AATGACTGCTTTTTATAAATTATGTGGAATGAGTATGCTTTCACTTGTATTAGCAGACTGCACATTCTTG
TCAGCAAATAAGCCTTATGATCGCGACCATGATGGTGAGTTAATTGTACATATGAAAGATGTCAATACTC
ATAAAGAGGTTGGAACAATCACAATATCACCATATATTCATGATGGTAACCAAGAAGGGATGTTGATTAC
GCCACATTTATATAATTTACCTGCTAATACTACTCATGGCATGCATATCCATATCAATCCTAGTTGTGAA
GATAATGGTATCGCAGCAGGTGGGCATTGGGATCCAGATAATACGCAAAAGCACCTTGGCCCATACAATG
ATAATGGACATAAGGGCGATTTGCCTGTACTAGTTGTTAACGCAGATGGTACAGCAACTGAGCCTGTTGT
AGCGCCAAAGCTTAATTCTCTAGAAGAGCTAGCGGGTCATAGTTTGATGCTTCATGCGGGAGGCGATAAT
TACTCTGATAAGCCACAACCTCTAGGCGGAGGTGGTGCTAGAATGTGGTGTGGAGTTATAGCAGACTA

Protein Sequence : Show Sequence

>gi|156501771|ref|YP_001427836.1| copper/zinc superoxide dismutase [Francisella tularensis subsp. holarctica FTNF002-00]
MTAFYKLCGMSMLSLVLADCTFLSANKPYDRDHDGELIVHMKDVNTHKEVGTITISPYIHDGNQEGMLIT
PHLYNLPANTTHGMHIHINPSCEDNGIAAGGHWDPDNTQKHLGPYNDNGHKGDLPVLVVNADGTATEPVV
APKLNSLEELAGHSLMLHAGGDNYSDKPQPLGGGGARMWCGVIAD

Molecule Role : Virmugen
Molecule Role Annotation : A sodC mutant in Francisella tularensis subspecies holarctica was attenuated and provided protection and cleared the infection against lethal challenge in nearly 50% of the mice infected with the ΔsodC mutant (Melillo et al., 2009).
Related Vaccine(s): Francisella tularensis sodC mutant vaccine

24. TUL4

Gene Name : TUL4
Sequence Strain (Species/Organism) : Francisella tularensis
VO ID : VO_0011076
NCBI Protein GI : 148688
Taxonomy ID : 263
Gene Strand (Orientation) : ?
Protein Name : 17 kDa major membrane protein (TUL4) precursor
Protein pI : 9.44
Protein Weight : 15568.2
Protein Length : 237

Protein Sequence : Show Sequence

>AAA24919.1 17 kDa major membrane protein (TUL4) precursor [Francisella tularensis]
MKKIIKLSLLSLSIAGLASCSTLGLGGSDDAKASAKDTAAAQTATTEQAAAVSKPTAKVSLNKLGQDKIK
ATVYTAYNNNPQGSVRLQWQAPEGSKCHDTSFPITKYAEKNDKTWATVTVKQGNNFCSGKWTANVVYDKE
VIASDSINI

Molecule Role : Protective antigen
Molecule Role Annotation : A 17-kDa lipoprotein, TUL4, of the facultative intracellular bacterium Francisella tularensis is one of several membrane proteins that induce an in vitro response in T cells from F. tularensis-primed humans. When mice were immunized with S. typhimurium chi 4072(pTUL4-15), some animals showed an antibody response and a T-cell response to TUL4. The present study demonstrated that the 17-kDa lipoprotein TUL4 of F. tularensis is involved in a protective immunity to tularemia (Sjöstedt et al., 1992).
Related Vaccine(s): F. tularensis vaccine X4072(pTUIA-15)

25. tul4

Gene Name : tul4
Sequence Strain (Species/Organism) : Francisella tularensis
NCBI Protein GI : AMD39429
Taxonomy ID : 119857
Protein Name : Tul4
Protein pI : 9.95
Protein Weight : 9890.45
Protein Length : 167
Protein Note : amplified with species-specific primers

Protein Sequence : Show Sequence

>AMD39429.1 Tul4, partial [Francisella tularensis subsp. holarctica]
MKKIIKLSLLSLSIAGLASCSTLGLGGSDDAKASAKDTAAAQTATTEQAAAVSKPTAKVSLNKLGQDKIK
ATVYTAYNNNPQGSVRLQWQAPEGS

Molecule Role : Protective antigen
Molecule Role Annotation : (Ashtekar et al., 2012)

26. wbtA

Gene Name : wbtA
Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
NCBI Gene ID : 3191939
NCBI Protein GI : 56708505
Locus Tag : FTT_1464c
Genbank Accession : AJ749949
Protein Accession : YP_170401
Taxonomy ID : 177416
Gene Starting Position : 1514679
Gene Ending Position : 1516415
Gene Strand (Orientation) : -
Protein Name : dTDP-glucose 4,6-dehydratase
Protein pI : 8.34
Protein Weight : 60861.77
Protein Length : 578
Protein Note : Similar to Q9S4V4 PglF from Campylobacter jejuni (590 aa). FASTA: opt: 1343 Z-score: 1435.9 E(): 3.9e-72 Smith-Waterman score: 1378; 43.543identity in 604 aa overlap

DNA Sequence : Show Sequence

>gi|255961454:1514679-1516415 Francisella tularensis subsp. tularensis SCHU S4, complete genome
ACTACCCATTCAATCTATGTTCAAATTCCGGAACAATTTTCTTTAATATCACAAGCTGATCAACATCATC
CTTGATCAACGATTCAATATCTTGGTTTAGAGTATTAATATCGTAAAAAGTCCTTCTACCAATAAAAATA
TCTTTATAGTCGGTACTAACATCATCTTCCTCTATCAAAAGCTCTTCGTAAAGTTTCTCTCCTGGACGCA
AACCAACTATTTTAATATCAATATCACCTCTACCAGAAAGTCTAATAAATTGTTTAGCAAGATCAATAAT
CTTGACAGGTTGCCCCATATCTAAGACAAAGACCTCTGAATTTTTTGCAATAGCACCAGCTTGTAGGACC
AGTTCACAAGCTTCTGGTATCAACATAAAATAACGTGTAATTTCAGGATGAGTAACTGTAACAGGACCAC
CTTTTCTTATTTGCTCTTCAAATTTTGGAATCACACTGCCACTACTACCAAGCACATTACCAAAACGCAC
TGCAGCAAGCTTGGTATTTTTGGGATCAACATTCTGTAAATACAGCTCACAAACTCTCTTGGTAGCCCCC
ATAACATTCGTTGGTCGCACTGCTTTATCAGTGGAAATCAATATAAATGACTCAACACCAGCTTCTATAG
CCAGATCTATAGCATTCTTAGTACCTAAGATATTATTTCTAATTGCTCTAGAGATATTCTCCTCAACTAA
GGGAACATGCTTGTAGGCAGCAGCATGAAATACTATATTTGGAGTATACTTTTGAAAAACCTCAGCCAAT
GCTTTTCTATCACAAACAGAACATAGCACACTATTGATATTAAAATGACTACACTCCTCAGTAATTTTAT
ATAAGTTAAACTCACTATGATCAACCAATATCAACTCTTTTGCCTGATACTTGATACATTGATGTACTAT
TTCAGAACCTATACTACCTCCAGCTCCTGTGACTAGCACCACCTTATTTTTGATAAAATTAGAGATAGAT
TCTTTATCTAAACTCTTAGTATCACGCGCTAATAGATCATAGAGTGAAACAGGCTTCAACTGTGACATAA
AATTCTCATCTTGAAGAATTTCCTCAAGAGGCGGCATAATTCTAATCTGATTAAAATCCTTTTCAAATTC
TTTATATATATTTTTGACTACTTGGTTTGCATTTCTTGGCAATGCAATAACCAAAAGGTCAAATCTACTA
GATAGCAATAGTTTTGTTAATTCAGCTTTAGATAGAACCTTTTTACTATCAATACTTCTTTTTTGTAAAG
TTTCATTGTCATCAACAAAACATTTGATGCGATAACCAGCAGAAGCAAGTTCTTGAGCAATCTTTGTTCC
TGCAGCACCTGCACCATAAATAACCGCAGTTTTACTTTTATCCACTGATCCTCTGTTCATCAAATACCAA
TAAAAGTAGACACTTAAACTGATCAAAAAAACATAAAATAAAAACTCAGAGAATATCAACGAAAAAGTGA
CTTTGCCATAAAAAAATAATGCCACTATGAAAAATACTGGCAAATTAATAAAAGCCTTACGTAAAAAAGT
TTTTTGAGTCGACTTACGCCAACTAGCCATATAATCTCTAAGTAGTAAAAAAGATGACAAGCATCTCAGC
AAAACTAATGCAAGTAAAAAATGTAAATTAACATCTTGCTTGAAAATATAGAAAGTCCAATTAACAGTAA
TAATAGTTAAAACTATTATTACCACGAAATTAAGCGTTCTATTATCGTAGAAAGACA

Protein Sequence : Show Sequence

>gi|56708505|ref|YP_170401.1| dTDP-glucose 4,6-dehydratase [Francisella tularensis subsp. tularensis SCHU S4]
MSFYDNRTLNFVVIIVLTIITVNWTFYIFKQDVNLHFLLALVLLRCLSSFLLLRDYMASWRKSTQKTFLR
KAFINLPVFFIVALFFYGKVTFSLIFSEFLFYVFLISLSVYFYWYLMNRGSVDKSKTAVIYGAGAAGTKI
AQELASAGYRIKCFVDDNETLQKRSIDSKKVLSKAELTKLLLSSRFDLLVIALPRNANQVVKNIYKEFEK
DFNQIRIMPPLEEILQDENFMSQLKPVSLYDLLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQ
CIKYQAKELILVDHSEFNLYKITEECSHFNINSVLCSVCDRKALAEVFQKYTPNIVFHAAAYKHVPLVEE
NISRAIRNNILGTKNAIDLAIEAGVESFILISTDKAVRPTNVMGATKRVCELYLQNVDPKNTKLAAVRFG
NVLGSSGSVIPKFEEQIRKGGPVTVTHPEITRYFMLIPEACELVLQAGAIAKNSEVFVLDMGQPVKIIDL
AKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDDVSTDYKDIFIGRRTFYDINTLNQDIESLIKDDVD
QLVILKKIVPEFEHRLNG

Molecule Role : Virmugen
Molecule Role Annotation : A wbtA mutant in Francisella tularensis was attenuated and provided protection in mice against challenge with an otherwise lethal dose of either F. tularensis LVS or a fully virulent clinical isolate of F. tularensis type B (Sebastian et al., 2007).
Related Vaccine(s): Francisella tularensis wbtA mutant vaccine

27. WbtI

Gene Name : WbtI
Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica FTNF002-00
VO ID : VO_0011074
NCBI Gene ID : 5522688
NCBI Protein GI : 156502002
Locus Tag : FTA_0635
Genbank Accession : CP000803
Protein Accession : YP_001428067
Taxonomy ID : 458234
Gene Starting Position : 590832
Gene Ending Position : 591914
Gene Strand (Orientation) : +
Protein Name : DegT/DnrJ/EryC1/StrS family sugar aminotransferase
Protein pI : 6.8
Protein Weight : 37905.36
Protein Length : 360
Protein Note : component of putative O antigen gene complex

DNA Sequence : Show Sequence

>NC_009749.1:590832-591914 Francisella tularensis subsp. holarctica FTNF002-00, complete genome
AATGAGTAAAGTAAATGTAACAAAACCATATTTACCAGATATAAATAAATATAAAAGCTATGTAAATAAA
ATATACAAAAATGGATGGCTTACTAATAATGGTCCGTTAGTGCAAGAGCTAGAAAAAAGACTTGCAAAGT
ATCTAGGTGTTAAAAATATAGTTTTAGTATCAAATGGTACAATTGCATTAGAAATCGCGTATAGAGCGTT
AGGAGTCAAAGGAAGTGCAATTACTACTCCATTTTCATTTGTCGCTACTACATCTTCATTGGTTTCTAAC
AATGTAAAACCAGTGTTTGTTGATATTGATGAGAATACTCTAAGTATAGACGTCTCTAAAATTAAGTATG
CTATTGAAGAGGATACTTCAGCTATTGTGCCAGTTCATGTGTTTGGAAATGGTTGTGAAGTTGAAAAAAT
AGACATGCTGGCTAAAAAACATAACTTAAAAGTTATTTATGATGCAGCACATGCTTTTGATGTTAAGTAT
AAGGGTGAGAGTATATTAAACTATGGTGATATTTCGACATTAAGTTTTCATGCAACAAAGATTTTTCATT
CTATTGAAGGAGGTGCGCTTATCATTAATGATGATAGTCTTGTTGAAAAAGTTCGTTATTTCATTAATTT
TGGTATAGAAAGCTCAGAATCAATACCTTACTTAGGTACTAATGCTAAAATGAATGAATTTGAGGCGGCT
ATGGGACTTTGTGTTCTAGATGATATTATAGAAATTAAGAGCAAAAGGAAAGTTATTACAGAGATATATG
AGGCTGGGTTAGATGGATTGGTAAAGTTTCAAGAACAGAATCAGCATTCTAGTAGGAATTATAGCTATTT
TCCAGTAATATTTAGGACTGAGGAGGAACTTCTCAGAGTACAGAAAGCACTAATACAAAATGATATAATA
TCGCGTAGATATTTTTATCCATCATTAGATAGTCTTAGTTATATAGAGCCAAAGCAGTATATGCCAATCT
CAAGAGATATATCTAAAAGAATATTATGTTTGCCAATTTATGCAGAGTTAGAAGACGATAAAATTAATAA
AATAATTAATAATATCAAAGAGGTTTCCTCATG

Protein Sequence : Show Sequence

>YP_001428067.1 DegT/DnrJ/EryC1/StrS family sugar aminotransferase [Francisella tularensis subsp. holarctica FTNF002-00]
MSKVNVTKPYLPDINKYKSYVNKIYKNGWLTNNGPLVQELEKRLAKYLGVKNIVLVSNGTIALEIAYRAL
GVKGSAITTPFSFVATTSSLVSNNVKPVFVDIDENTLSIDVSKIKYAIEEDTSAIVPVHVFGNGCEVEKI
DMLAKKHNLKVIYDAAHAFDVKYKGESILNYGDISTLSFHATKIFHSIEGGALIINDDSLVEKVRYFINF
GIESSESIPYLGTNAKMNEFEAAMGLCVLDDIIEIKSKRKVITEIYEAGLDGLVKFQEQNQHSSRNYSYF
PVIFRTEEELLRVQKALIQNDIISRRYFYPSLDSLSYIEPKQYMPISRDISKRILCLPIYAELEDDKINK
IINNIKEVSS

Molecule Role : Protective antigen
Molecule Role Annotation : Immunization with WbtI(G191V) protected mice against a relatively low-dose intraperitoneal challenge with LVS (F. tularensis subspecies holarctica live vaccine strain), but was less protective against a high-dose challenge (Li et al., 2007).
Additional Molecule Role : Virmugen
Additional Molecule Role Annotation : A wbtI mutant is attenuated in mice. The mutant protected against a low dose challenge with the parental strain, but was less effective against higher doses. However, increasing the immunization dose increased the resistance to higher challenge doses (Li et al., 2007).
Related Vaccine(s): Francisella tularensis wbtI mutant vaccine

III. Vaccine Related Host Genes

1. Ccl2

Gene Name : Ccl2
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 20296
NCBI Protein GI : 6755430
Locus Tag : RP23-350G1.3
Genbank Accession : AJ238892
Protein Accession : NP_035463
Taxonomy ID : 10090
Chromosome No : 11
Gene Starting Position : 82035576
Gene Ending Position : 82037451
Gene Strand (Orientation) : +
Protein Name : chemokine (C-C motif) ligand 2
Protein pI : 10.38
Protein Weight : 14837.89
Protein Length : 148
Protein Note : Also known as JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594

DNA Sequence : Show Sequence

>gi|372099099:82035576-82037451 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
TGCAGAGAGCCAGACGGGAGGAAGGCCAGCCCAGCACCAGCACCAGCCAACTCTCACTGAAGCCAGCTCT
CTCTTCCTCCACCACCATGCAGGTCCCTGTCATGCTTCTGGGCCTGCTGTTCACAGTTGCCGGCTGGAGC
ATCCACGTGTTGGCTCAGCCAGGTGAGACCCCCCCAGCTCCCTTCCCAGCATATCACCCCATTTTTGAAT
TGTCGTGGATTGTGATAGCATAGTCTCACATGGTCAGGTACTTTTTTTTTTTTCTTTAACCAAGATAAGG
AGCATAAAGAAGGAAGGACAAAGAGCCAACCCAATTACAAGATTGCTTCTGGAAAGCAACTAGAATTTTA
ATTGTTAGATCTAAATTTGGAATCACACCTTCATATAGTTCCTGTTCCAGTTACTTCCCTCAGTATTTGG
GAACCTGGGTGATCAAACAGAGGCTTGGGTTGGTGCCTTTTTCCAGATAGAGGAGAAAGGGGAAGAGATC
CAAGATCCGAGCTGTGTTTCACCCAGCCCTGCTTCCAGAGATAGCAGCTTAGCGGAGGTGGTTGGGATCA
GAGATACTCATGATTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAGCCTACAGTAATGTA
CTCAGGTAATCTTCTCAGGTCATAGTAATTTGACTTCTAACTCCCCCAAATGACAGTCCCCAGAGTCACA
TAGTTTTAATGGCATCCCTCTACCCAAGACTGTGAGCCTACTTTAAGCTGCAAATAACTGAGTCTGTTGT
CAAAGATCACATCCCAGATCTGATGTATTGGCATTTATATCCCATCCTGCTGAAACTGCCTTCTCCCCGT
GGTCCTTCTCTTCTCTAAGGTCAGAAGCATCTTTCCTGTCCTAATGTGCTTCTCTTTTACTCTCCAGATG
CAGTTAACGCCCCACTCACCTGCTGCTACTCATTCACCAGCAAGATGATCCCAATGAGTAGGCTGGAGAG
CTACAAGAGGATCACCAGCAGCAGGTGTCCCAAAGAAGCTGTAGTGTGAGTTACATACCCCGGCCCTCCC
TGGTCCAAAGGTTTTTCCTTAAGAACAAGGGATGGTCCTCATATACTTATAGTCAGTCACACACTCAGAT
CCAATGGGGAAACCAAGGCCAAGAAGGCAAAGGCAGTTCTCAACAGCATTGTCTCTATGGCTGCTGTTCA
GGCCCTTTCTACTCCACAAGCTTATCTTAGAAAACCTGCAGGAGAAGCAGGTCACTTTGAGTCCCCTTTT
TCTACCTGCCCTCCCCCGCTGAGCTCTACACAGCCCCTCCATGTATACCAGACTGAACTTCATCTAACAG
TGTCTTTTCTCTTCCCACAGTTTTGTCACCAAGCTCAAGAGAGAGGTCTGTGCTGACCCCAAGAAGGAAT
GGGTCCAGACATACATTAAAAACCTGGATCGGAACCAAATGAGATCAGAACCTACAACTTTATTTAAAAC
TGCATCTGCCCTAAGGTCTTCAGCACCTTTGAATGTGAAGTTGACCCGTAAATCTGAAGCTAATGCATCC
ACTACCTTTTCCACAACCACCTCAAGCACTTCTGTAGGAGTGACCAGTGTGACAGTGAACTAGTGTGACT
CGGACTGTGATGCCTTAATTAATATTAAACTTATTTAACTTATTGATGTTATGGTATTCCCTTTCATGAA
TACTAAAATTTCTTAAATGCAAGGTGTGGATCCATTTTTCCCTCTCTGTGAATCCAGATTCAACACTTTC
AATGTATGAGAGATGAATTTTGTAAAGATGAATGGGTAAACTTTGTGTTTGAGATTCCAAGGTATTGTTT
AAAATATTATTATGGATATTCCTTATTATTAAAAGAAATATATTATTTTTGTACAC

Protein Sequence : Show Sequence

>gi|6755430|ref|NP_035463.1| C-C motif chemokine 2 precursor [Mus musculus]
MQVPVMLLGLLFTVAGWSIHVLAQPDAVNAPLTCCYSFTSKMIPMSRLESYKRITSSRCPKEAVVFVTKL
KREVCADPKKEWVQTYIKNLDRNQMRSEPTTLFKTASALRSSAPLNVKLTRKSEANASTTFSTTTSSTSV
GVTSVTVN

Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis FTL0552 mutant vaccine

2. Ccl5

Gene Name : Ccl5
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 20304
NCBI Protein GI : 164698428
Locus Tag : RP23-320E6.2
Genbank Accession : AB051897
Protein Accession : NP_038681
Taxonomy ID : 10090
Chromosome No : 11
Gene Starting Position : 83525777
Gene Ending Position : 83530517
Gene Strand (Orientation) : -
Protein Name : chemokine (C-C motif) ligand 5
Protein pI : 8.73
Protein Weight : 9475.12
Protein Length : 91
Protein Note : Also known as SISd; Scya5; RANTES; TCP228; MuRantes

DNA Sequence : Show Sequence

>gi|372099099:83525777-83530517 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
CTTTGTCCTTGCATCTGAAATTTTAATGAAATGCTGATTTCTTGGGTTTGCTGTGCAGAGCCTCGGAGCA
GCTGAGATGCCCATTTTCCCAGGACCGAGTGGGAGTAGGGGATTACTGAGTGGCATCCCCAAGCTGGCTA
GGACTAGAGCAAGCAATGACAGGGAAGCTATACAGGGTCAGAATCAAGAAACCCTCTATCCTAGCTCATC
TCCAAATAGTTGATGTATTCTTGAACCCACTTCTTCTCTGGGTTGGCACACACTTGGCGGTTCCTTCGAG
TGACAAACCTGCAGGAGAGAGGAAGGTCAACACTGGGGCAGCGTTTGGTGTACGGGCACGGAAAAGCATA
AAGGTGACAAGAGCCGGGGAGTGCGTTTTTGTAGCTCCATACTGTTTTCTGCCTTTTAGAGGGTAAGTCT
TCTCCTTTGGAACCAGAGGCACTGAGCTACAGGCAGAGTTCCATTTGGGTTAATTCCCTACAAACACACA
GATTTGGAACCCAGCATTGAGCAGGCCAAGCTCTGGCCTTTTCTTGGCCCCTTTCCTTGCTCCAGGCCTT
ATGCTGAGTGACGAATTTAGAGACGTTGCGTTTGATTCACTTAAATCAGTTGAGTTGAGGAAATTAAGAG
GGAACTCTAAGACTGCCTAGTTGTTGCTCTTGGCATTCTAAGCCTGTAGCCAGGGAGTCAAAAGGCTAGG
ATACCAACTCCAGCTATCAAGATTGCTCAGGCCCCCATTTCCCCGTCCTTTATACAGTATGTGATACCAA
AGAACAAATCTCCTTTGCTAAGTCTTTCCTTCTTTCCTCCACTTTTGGAGCCTCTGTTCATCTGACTTCC
CCTTCCACCTGTCTCTTAAGAGCAGGTGTCCTCAAAGAAATTGTCCCCCTTACCTTCTCCCTTTTCCAAT
CTCTGGCCCCAGGCCTGCATATAGTTGATGCTTGGTGAATATTGGATGAATGACTGGATGAAAGAATCAC
ATTCATTCATTCTTACCACATGTATGCATTCATGTGCGCACGCACACACACACACACACACCTGTGCACA
CATGTGTGCGCACACACACAGACACACAAATAATATCAATAGTAAGTTGATACTTCTTTATACCCATTAT
GTGCCAGGGAATGGTCTAAGTGATTTTTTTTTTCTGTGTCCTAGTGAAAGAAATGCCTTAATTATCCTCT
TTTGGAACTACCTCAGAGAGGTTGAGGGGCCCCAGTTTATCAAACAACATAACTAGGATTCTAGCATGCA
GACCAGCTGGGTGGCCAAGGATGACTAAGACAATGTTGCCCAGCCTCTTTTATTGGCAGTTCCTGGACTC
TGTCTTCAGCCCTTGGGTTGCACTCCATCTGGCATCTCCATGGATTATCTTGCTGGAACTTTGCTTGGTT
CCTTCCTCTTTACACAGTTGGCCCTCTAGACCTGCACACCTGCACACCTCTCCGTCTCTGACTGGGAACC
TCAGCATCTGCATCTCTTCTGTCCTTGCTCCTGACCACAAGTCCCTCACTCTGGCTGACTCATCCTCCCA
CACTCCTGACTCTTGTTTCTTCTGGCCTCCAGGTACCACCACTCTGCTGACAATAGTACACGTGTGGGTG
ACTTCAACCGCCTCTTCTCCAGACCCCCTCCTAGGCTTTGTCTGGACCTGGTCATTTTACTCTTGCCAGC
TCTAGGTTTTGCTTCAGTTTCCTGACACTTGAGGTGCCCTCTGCTTTCCTACATATGTCCCTACAAACCA
ACGTAGGTGTCCATGTGTTCTCAGATACACCCGTGTCACACACATCAGATACACCCATGTCACACACACT
TCTCTACTCCTAATCTTGACACAAATAGCATCTTTTTACAGTAATCACCTGCCTCACCCTTGAAGCTAGA
CACACCCCCTCCTCTCCTCTAGTATGGTTTTATAGGGTGCCGTAGATCATTCTGTGTCCAACTTATCAGT
CTTACTAGATGAAATTTCCATAGCCAAGGATGACAATTTTATTCATCTCTGCCGGCTCATAATAGGTTCT
CAAGGAAGGATTTTCTTTAAAAAGTTTTATTTTAGAGCTAAATTTGGTAATATTAAATAGTAATATTACA
CTGAAGTTCATCTTTGCCAGGGTTAAAAAAAGTCATTGGATTAAAACTCTTCAACCTTTTATTGGAAACT
TTATGATTTCTTCTTTTTCTTTTTTACATTTATGTAGATGAGTATTCATCTGCATGTATTTGTGCACCAC
ATGTGTGCCTGAGGAGGTCAGAAGAGGGTATTGGATCCCCTGGAACTGGAGTTACAGAGTGAGCCATTAT
GTAACGAGTGAGCCATCATGTAACCAGTGAGTCACCATGTAACGAGTGAGCCGCCATGTAACGAGTGAGC
CACCATGTAACCAGTGAGCCACCATGTAACGAGTGAGCCACCATGTAACGAGTGAGTCACCATGTAACGA
ATGAGCCACCTTGTAACGAATGAGCCAACGTGTGGATGTTGGGATGGAGGTTGATCCTCTGCAAGAGCAA
GCAGTGCTCTTAACCATGGAGCCATCTCTCCAGCTCTCCGTTTTCACTCTGTAAATCGTTATCATCTTAA
GCTTCTTAAGCTATCCTGTCACTAATGACTTTATTTTAATACAGGAAGTAGGTCCAGAGACAGACTGCAT
CCCTCACTTACTGTGCAGCTTTGAGTAGGCCTGCAAATGCCTCTGAATCTGTTTCATTACGTGGAGAAAT
TTGTGCATACCCGCCAGGGCTATGCACAATTTAGAGACAGTACACATAGACACAACATGCCAGCAAGCAC
CCTATTATATTCCCCAAATCTATCAATATGCATATTTAAAGCTGAGTCTCATTATAACTTCTTAAATGGA
TAGGGTTTTAGATTTTATTTACTTGATTGAAATTAGGGAACTGACCTTGATCCATCCTCCCTAGTTCCTC
AGAGGATTAGGCAAAGGTTGGTGTCCTAGGAACTGTGTCTATCTCTATAGGCAGGAGCCTCTAAGGCTAC
GGTTCTCAACCTTCCTAATGCTGCGGCCCTTCTCATGTTGTGGTGACCCCCAACCATAAAACTATTTTTG
TTACTGTTTCATAATTGTAATGTTGTTACTGTTATGAATTGTAATGTCAATATTTTTGGAGATAGAGATT
TGCCAAAGGGGACCCGACCCACAGATTGAGAACTGCTGCTCTAGGGCTCCCTGTGTCTAGTCCTTTAATG
TTACAGTTGGAGACTCTGAGCTGGAGACGAGGAGCCATAGCTATTAGAGGAGCCCAGAGAGTGAGTGGGA
GTGAGAGGGGGCACCCCTGAGAAGGATCACGGCGGGGTTGTGGGATGGCTTCTATACCCCACAGAGGAAC
CCATGGGGCTGAGACTCACACGACTGCAAGATTGGAGCACTTGCTGCTGGTGTAGAAATACTCCTTGACG
TGGGCACGAGGCAGCGCGAGGGAGAGGTAGGCAAAGCAGCAGGGAGTGGTGTCCGAGCCATCTGGGAGAA
GGAAGAAAGGAAACCCTTTTATTCATTGCCAGCTATGCTGGGCTCTTTACACGATTTATCTTAAAAGCAG
TGACTGTCGTTGTCGACCTTGGCAGAAATGGCTCAGAGGACACATTTGCCATGGTGGAGAGTTCAACAGA
AGCCTGTTTTGTCTGGTCCGTGAGCCAAGCCTGTGCCTTGTGCTCTGTACCGTAAGCCCCTGCTGGGATC
CCTCTCCTCGCTCTGTGCCTTCTGCCCTTAGCAGGGCTTTCAGAGCTCCCCTGGTGGATTCAAAGCTTGG
TGTCTTCGAGGTGTAGCATGAGTTCTGGGGGAGTGCCCAAGCCGAAGCTCCATAACTTAGTGACGATCCT
AAGAGGGGCCTGAAAGTGCACATAGCATCCTGGGACCTGGCTGGCCTGTAGCGAGGGGCTCTGACCAGAG
AAGCTCTTGGTGGTCACTTAATTCATCCCACAGCAGAGCTGTGGTGCTTAGCTTCCCAGTCAGATAGGGC
TTTTGGAATACCAGTTTCCACAATTGTAGCAGTGTGCACCCTTAGACAAGAATCTCAGTGTTCTTAGGCC
TCTGTTTCCCACTGCAACACAAGAGCAACTACTGAAAGACTATTTGTATTAAACAGGATGGCACATTAAA
CTCATTTGCACAGTGCCTGACCCACGGTAGGTACTTAGTTCACTATCTATCTATCTATCTATCTATCTAT
CTATCTATCTATCTATCTATCTACCTATTTATTTTGCAAGCCCCATTTCTTTTCTCACTATGAAATGAAA
GACCATCCTACCTAAAACAGTATCACACATTTCTTAGAAACCACACTGGTGACAGATATGAGCAAGTCAT
TTTTCAAAGGTCAGCCCGGCACCAGTTCACAATAAGGAGTGCCTGCAGAATTCATAATATCATCTTTGAA
GACACAGAAAAGTTCCTCAGAGGATCCTGCCATTAGTATACAAGACATCTAACAGTGATGACAGGGTAGC
CGTGTCAAGGATGCGTAAGAATGTACAGCAGCATACACAGTAGGATTGGAAAGAAGGGGAGGTCTGGGTA
TCAAGATGGGTTGGCATCGAGGCAGAGTGGGGCTGTGATGGTCAGAACCCTCGACTTACATGGTGAGGCA
GGTGCGGGGGTGCAGAGGGCGGCTGCAGTGAGGATGATGGTGAGGGCAGCTGCAGAGATCTTCATGGTAC
CCGCGGCAGAGGCTGTGGGAGATGCATGTGCTGTCTCAGAGTCCTCTGCAA

Protein Sequence : Show Sequence

>gi|164698428|ref|NP_038681.2| C-C motif chemokine 5 precursor [Mus musculus]
MKISAAALTIILTAAALCTPAPASPYGSDTTPCCFAYLSLALPRAHVKEYFYTSSKCSNLAVVFVTRRNR
QVCANPEKKWVQEYINYLEMS

Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis guaB mutant vaccine

3. Cxcl1

Gene Name : Cxcl1
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 14825
NCBI Protein GI : 6680109
Genbank Accession : AC157938
Protein Accession : NP_032202
Taxonomy ID : 10090
Chromosome No : 5
Gene Starting Position : 90891244
Gene Ending Position : 90893120
Gene Strand (Orientation) : +
Protein Name : chemokine (C-X-C motif) ligand 1
Protein pI : 9.62
Protein Weight : 9757.89
Protein Length : 96
Protein Note : Also known as KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1

DNA Sequence : Show Sequence

>gi|372099105:90891244-90893120 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
CAACAGGACTCAGAGCCTCTAACCAGTTCCAGCACTCCAGACTCCAGCCACACTCCAACACAGCACCATG
ATCCCAGCCACCCGCTCGCTTCTCTGTGCAGCGCTGCTGCTGCTGGCCACCAGCCGCCTGGCCACAGGTA
GGTCTCGCCACTGCCACTGGGGGAGGAGGGACCTCTGGTGAGCGCAGCCTCCCACAGTCCCGCTGACCAA
GAGTCTTCTCCCATAGGGGCGCCTATCGCCAATGAGCTGCGCTGTCAGTGCCTGCAGACCATGGCTGGGA
TTCACCTCAAGAACATCCAGAGCTTGAAGGTGTTGCCCTCAGGGCCCCACTGCACCCAAACCGAAGTCAT
GTGAGTATCTTCCCGGTTAGCTTCTGCCACTTCCAGACTCGCCCAAACCCTCCCGCGCCCCCACACTTCT
CCTAGTGGGAATGCCTAACATGTGGGTCTATCCTTCTCTCTGCAGAGCCACACTCAAGAATGGTCGCGAG
GCTTGCCTTGACCCTGAAGCTCCCTTGGTTCAGAAAATTGTCCAAAAGATGCTAAAGTGAGTTGTGACTT
GTGTTTGTACCCGAGACTAGAGTCAAGGGTGGGAGTACTGGCATATGACCCCTGAACGTTAATTCTATCA
AGGAAAGTCTGTGTTTATCAGGAGGTCGGAAAGTTGTGGTTATTATTTTGGTAATTGACCTTAATTTGAG
ATCATTAATTGGTTCTGATGCTAGAGAATTGTGTCCTCTTGGACACAATTACTATTGTATATTACACAAT
TACTATTGTGTATAATTATTACCACACTCAAAACCCCTATGCAAATGGTCAGTGGCAACAGTTCCTAAAA
GATGGAGAAGTCGCCAATAGCCTTTAAAAGTCTCCTTAGGGGCTGGCAAAGCATGCCCGAGCCTTCTTTT
GCCAGTGAGATTTCCCTGAGTTCAAGGCTAAGGTCTCTGTGAATGTTACATAATTTATTGAATTTAAGCC
AAGTAGGGGAGATAATAGCAGCTATATCACAGAGTTGTGAGTGAGAGAGGATTAAAGTCACACTTGTAAA
TTAACCCATGCTGAAGTAGGTTTTTGTTTGTTTGTTTTTGTTTTTGTTTGTTTTTGTTTTCCAAGTCAAG
TGATTGACTCCACAGGAACCGACAGTACAGAACTCTGGGTGCAAGTGTAGACATGTAGAAAGCCCATCTG
GAGCCTCAGGGGCTGGAATAAAAGAAGAAAGGCTTTGATGACTATTTGTAACAATGACTCTTTTCTGATT
GCAGAGGTGTCCCCAAGTAACGGAGAAAGAAGACAGACTGCTCTGATGGCACCTGTCTGGTGAACGCTGG
CTTCTGACAACACTATACAATTTCTTTTGAGGGTCCTATTTATTTATGTATTTATTTATTCCACAAAGTG
TGTGGTTTTTATTTTACATTAATATTTAACAGTGTGGATACATTTCATCGATGGTAGTTCAGTTCTGCTT
GTTCAGTTTAAAGATGGGTAGGCTTAAAATATTTCATTAAACTAATATTTATTGGGAGACCACTAAGTGT
CAACCACTGTGCTAGTAGAAGGGTGTTGTGCGAAAAGAAGTGCAGAGAGATAGAGTTTAGTATTATGTTT
TGTATGTATTAGGGTGAGGACATGTGTGGGAGGCTGTGTTTGTATGTCTTGAAAAGAATGTCAGTTATTT
ATTGAAAGTCGTCTTTCATATTGTATGGTCAACACGCACGTGTTGACGCTTCCCTTGGACATTTTGTGTC
TAGTTGGTAGGGCATAATGCCCTTTTACATTCTTTAACCCTGTTTCTCCTGGTCTCGTCTCGCTCGGGAC
AGAGACGTTCAAAGGACTGTTACAAATGAAGTAAAAATAAAAGTTTTATTAAAAAAA

Protein Sequence : Show Sequence

>gi|6680109|ref|NP_032202.1| growth-regulated alpha protein precursor [Mus musculus]
MIPATRSLLCAALLLLATSRLATGAPIANELRCQCLQTMAGIHLKNIQSLKVLPSGPHCTQTEVIATLKN
GREACLDPEAPLVQKIVQKMLKGVPK

Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis guaB mutant vaccine

4. Ifng (Interferon gamma)

Gene Name : Ifng (Interferon gamma)
Sequence Strain (Species/Organism) : Mouse
NCBI Gene ID : 15978
NCBI Protein GI : 33468859
Genbank Accession : NM_008337
Protein Accession : NP_032363.1
Other Database IDs : MGI:107656; UniProt: P01580
Taxonomy ID : 10090
Gene Strand (Orientation) : ?

DNA Sequence : Show Sequence

>gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
AGTATACCTTTGCAACC

Protein Sequence : Show Sequence

>gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
LLPESSLRKRKRSRC

Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
Additional Molecule Role : Vaximmutor
Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
Related Vaccine(s): F. tularensis FopB Protein Vaccine , Francisella tularensis FTL0552 mutant vaccine , KKF24 , LVS

5. IgG

Gene Name : IgG
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16059
Genbank Accession : AF010213
Taxonomy ID : 10090
Chromosome No : 12
Gene Starting Position : 881411
Gene Ending Position : 914690
Gene Strand (Orientation) : +
Protein Name : immunoglobulin heavy chain (V7183 family)
Protein Note : Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)

DNA Sequence : Show Sequence

>gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
ACAAGCACAGTGCAAATTTCTGTCACCCTG

Molecule Role : Vaximmutor
Additional Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis capB mutant vaccine

6. Ighg1

Gene Name : Ighg1
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16017
Genbank Accession : AC160982
Molecule Role : Vaximmutor
Related Vaccine(s): KKF24

7. Ighv1-9

Gene Name : Ighv1-9
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 668478
Genbank Accession : AC073561
Taxonomy ID : 10090
Chromosome No : 12
Gene Starting Position : 114583568
Gene Ending Position : 114583861
Protein Name : immunoglobulin heavy variable V1-9
Protein Note : Also known as Igg2a; Gm16697

DNA Sequence : Show Sequence

>gi|372099098:114583568-114583861 Mus musculus strain C57BL/6J chromosome 12, GRCm38 C57BL/6J
GTCTTGCACAGTAATAGATGGCAGAGTCCTCAGTTGTCAGGCTGCTGAGTTGCATGTAGGCTGTGTTGGA
GGATGTATCTGCAGTGAATGTGGCCTTGCCCTTGAACTTCTCATTGTAGTTAGTACTACCACTTCCAGGT
AAAATCTCTCCAATCCACTCAAGGCCATGTCCAGGCCTCTGCTTTACCCACTCTATCCAGTAGCCAGTGA
ATGTGTAGCCAGTAGCCTTGCAGGAAAGCTTCACTGAGGCCCCAGGCTTCATCAGCTCAGCTCCAGACTG
CTGCAGCTGAACCT

Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis capB mutant vaccine , Francisella tularensis wbtA mutant vaccine , KKF24

8. IgM

Gene Name : IgM
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16019
Genbank Accession : AC073553
Taxonomy ID : 10090
Chromosome No : 12
Gene Starting Position : 171229
Gene Ending Position : 175133
Gene Strand (Orientation) : -
Protein Name : immunoglobulin heavy constant mu
Protein Note : Also known as Igm; muH; Igh6; Igh-6; Igh-M; AI326478; MGC107036; MGC107507

DNA Sequence : Show Sequence

>gi|121699722:171229-175133 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
GTCATTTCACCTGCAGGTGACAGAGACAGTGTGAATTGCTGTCCACTTGTAGGGAGTAGAGGTAGAGGCA
GTGAGGACTGGTGACTCCCTGTCCCAGCCCTGTGTCCTCAGCCCCACAACCACACTACCTTGAACAGGGT
GACGGTGGTGCTGTAGAAGAGGCTCAGGAGGAAGAGGACGATGAAGGTGGAGGCAGTGGTCCACAGGTTC
TCAAAGCCTTCCTCCTCAGCATTCACCTCCCCCTCTATGAAGGGACACAGAGAGGGAGGGTCAAGCCAAG
TCTTGGGGTGACTGAGCATTTGCATAAGGGTTGGGGTGCCCTGATGGTTCTGGAGAATCTTAGCGAGTAT
TGACTGGTCCCCTAATAGCTCAAGCTAGACGGAGGCACAGAACAGGCCAAGCTGTAGGACACACTAATTT
GGAGCCTTTGTTCCAGGAGCCCTGGGGCAGCAGAGCAAGCAAGACAGATGCAATGTATGGACCTGGGTAA
AGGCAGACTCAGGAACTGTGGCAAAATGATATTGGTGTGCTCTGGACTTTCCCTGAGCAGGTGGTGCCAT
CGGTGCTGTTTAGACTGAGAATTTAGAGCAGGTCTCCCTGTCTCTTGGGCTTTAGCAATAAGAACAGGGA
TACCAGAGGTTGTCCCTCCTTACTTCCTGAAGGACTCAGGCATCTCATGACTTTCTTTGATCAGAGTTCC
CGTGCGTTGCTTGTCCTACCCCTGCCAGCAATGTTGGGTCTTGGTACCAAGAGATAAACAGACAGATACC
TGAGGGAGGCTGTGGGACCAGGGCATTTATTGGGATCTCTGATCCCTGTAAAGATGAGCCCTTGGGGATA
TCTAAAGCCTAGGCCCCAAATGGATATGAATATGAAGAATGTCCAGGATATGAGCCCAAAGAGGCAGTGT
TATTGGGTGGTGACTCCTTACAGAGGCTCCTTGATGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
GTGTGTGTGTGTGTGTGTGTGTGTGTGGTTGGGGCATCTTTTGGACAACTGCATTATGTTTTCTGCATTT
TGAACTGGAGATCAGGGTGGGTGTGAGTGGGTTAGGCAATTTCACATTGGTCTTCTGAGGTGTTCTATTA
ATGGAGACACAGCCAAGGCTAACTTTCTGTCATGGGTGGTTAGTGGAGTATCTTCTGCCCCATGGTGTTG
AGACAGATAGGCATACTTTGTACCTGGGGTTATCTCTCCCAATCATAGAGTGGGCTCCTGAGGAGTATCA
GTGACCCAGCCTAGTGTAGGCTCTCTGAGCCGTGAGCCTTGGAGATTACAGCCCTGGTCAGAGTCCAGGT
GTATCTGTGTGCTATACCTGTTCACATGTTAGTGTACACATGTGAAGATGTTTCTGTACATGTGTGTATA
TGCATATCTTTGTATCTATATAGTTTATTTGTGTATATTTGCCTATAGTCTGTTTCTGTGTACACAGGAC
CATGCATATTGGTATCTGTTTCTGTGAGTGGTTGTGTGTCTATGCCTATTTGTCTCTGAAGGCGTTTGTA
AGTGTCCATGCATGTGTGTGCGCATGAACTCATGTGTATATATGTGTACCTTTGTCTGTGTGTCCATGAA
TACACATTCATGTATGTGTCTACACATGTGTACCTATATATCTATGTATAAATGCATGTGTGTCTATATA
TGTGCCTGAATGCTGCCTGCACCAGGTTAGGCAAGCTTTCAGCCATATATCTAGAAGTAGCTGGAGGAAT
CGCATGTTCATACTCTGTCTGTTGTAAGCCTGACTGAGTTCACACACAAGGAGGAAACTGGCAAGGTAGA
GGAAGGGCCAGGGTGGAGGCAAGTATGCAGGGTGTGTGCAGAGCAAGCAAAACACAACTGTCTCAGCAGG
CATGAGCATTGTATAATCAAAACCAGTGACGTTTGAATGGATTTTTTTTATTTCTAATTTTTATAAAATG
CAACATCTCACTCTGACATGGTTAGTTTGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGA
GCGCTAGCATGGTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTACAGTGTGGGT
TTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGTGGCAGGGCCTCGTGGCCTACAACACAGG
TATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGCTGTGGGTAAAGTAGAAGCCTGG
GGCCCCAGGCTCTGGCATCGGGGCACTGGTCACATACTTCTCTTGGGGCAAGAGTTGCCCTCTCTGAAGC
CACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAGGCAGGTGACTGTGGCTGACTCCCTCAGGT
TCAGTTGCTCACGAGCTGGTGGCAGCAGGTACACAGCAGGTGGATGTTTGTGCACCTCTGTAAATGAAGA
CAGTAGTGAGGATAGGGTGGAAGAGGACCTGCCCTCCCTATGAGGTACAGGAAGGGTCCTGCAATTGGAG
GGGAAGGGAAGGGGGGATACCTACCATTGGGTTTTGAGATGAATTTCTTCTGTGGTGAAGGCAGATCCCT
GTGAGTCACAGTACACACAAATTCCTTCCTGTTATTCCAGTCTTCCACACAAACACTAGCCACACCCTTA
GCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTTGGTTTCCAGTGGTTCACCACTTTGAG
AAGCCCAGGAGATATTCAGGGTTTCATAGGTTGCCAGGTTTGAGACCAGACAGGTCAGGTTAGCGGACTT
GCTGAGGAAGATGTCGGCAAAGGAGGGGGGGATGGTGAAGGTTAGGATGTCTGTGGAGGGACCTGCAGTC
AAGAGAACACTGTGTCAGGTTATGACATATCTAGTTGTGAAGTTGGAAAAAAGCTTAGTCTCCATCCCCC
AAACCTACTTAAGGGAAGATGCTGGTCCTTCCAGCTGATTCTCACCCCTCTACTGCCTATTGCTCATTCT
TGTCCTTGGCCACTCTGGGGAGCCAAAGTTCAAGGAGCAAATGACCATGTCTGGGTGGCATTGGCCATAA
TTGTAGGAGGACTTCCCTAATCTGGGAGGGCTAGGCATTGGGCTTAGCCCAGGCCACTCACTGGCAGCAC
ATGTGGAGGACACGTTCTTCAAGAAGGTGAGACCCCTGTGATCCACACGGCAGGTGTACACATTCAGGTT
CAGCCAGTCGATTTCAGAGATGGTAAGTGTGCTTATGACCTTGTAGGTTTGGGGTGTGGATCCTTTGTTC
TCGATGGTCACCGGATCTGTGGTGAAGCCAGATTCCACGAGCTTCCCATCCTTTAGCCAGGATACTGTGA
TCGGTTTTGGAGTGAAGTTCGTGGCCTCGCAGATGAGTTTAGACTTGCGTGGTGCAGGGCCAGAGAAGCC
ATCCCGTGGTGGGACGAACACATTTACATTGGGGTTCATCTCTGCGACAGCTGGAATGAAAGGTCAAGGT
GTGAGCTCAGCTTGGCCTAAGCCCCACCCCGCTGCTCCCTCTGGGCCATGCCTGGGCCTGGGCACCCCTG
CTGGGAGGGTTTGGTTCTTACCTGGAATGGGCACATGCAGATCTTTGTTTTTGCCTCCGTAGTGGATTTT
GCATACCAGGTATTCATCTGAACCTTCAAGGATGCTCTTGGGAGACAGCAACACCTGCGAGGTGGCTAGG
TACTTGCCCCCTGTCCTCAGTGTTGGGAAGGTTCTGATACCCTGGATGACTTCAGTGTTGTTCTGGTAGT
TCCAGGTGAAGGAAATGGTGCTGGGCAGGAAGTCCCGGGCCAGGCAGCCCATGGCCACCAGATTCTTATC
AGACAGGGGGCTCTCGCAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTC

Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis capB mutant vaccine

9. IL-1b

Gene Name : IL-1b
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16176
NCBI Protein GI : 6680415
Locus Tag : RP23-384K11.2
Genbank Accession : AL808143
Protein Accession : NP_032387
Taxonomy ID : 10090
Chromosome No : 2
Gene Starting Position : 129364578
Gene Ending Position : 129371119
Gene Strand (Orientation) : -
Protein Name : interleukin 1 beta
Protein pI : 4.31
Protein Weight : 29045.55
Protein Length : 269
Protein Note : Also known as Il-1b; IL-1beta

DNA Sequence : Show Sequence

>gi|372099108:129364578-129371119 Mus musculus strain C57BL/6J chromosome 2, GRCm38 C57BL/6J
GTTTGTTTGTTTTAATGAAATTTATTTCATACTCATCAAAGCAATGTGCTGGTGCTTCATTCATAAAAAG
TCTCATCCATATTATAGGAACCTATTTATTCTCAGCTTCAATGAAAGACCTCAGTGCGGGCTATGACCAA
TTCATCCCCCACACGTTGACAGCTAGGTTCTGTTCTAGAGAGTGCTGCCTAATGTCCCCTTGAATCAACT
TAAATAGATCAACCAATCAATAAATACATAAATAAATAGGTAAGTGGTTGCCCATCAGAGGCAAGGAGGA
AAACACAGGCTCTCTTTGAACAGAATGTGCCATGGTTTCTTGTGACCCTGAGCGACCTGTCTTGGCCGAG
GACTAAGGAGTCCCCTGGAGATTGAGCTGTCTGCTCATTCACGAAAAGGGAGCTCCTTAACATGCCCTGG
GGAAGGCATTAGAAACAGTCCAGCCCATACTTTAGGAAGACACGGATTCCATGGTGAAGTCAATTATGTC
CTGACCACTGTTGTTTCCCAGGAAGACAGGCTTGTGCTCTGCTTGTGAGGTGCTGATGTACCAGTTGGGG
AACTCTGCAGACTCAAACTCCACTTTGCTCTTGACTTCTATCTTGTTGAAGACAAACCGTTTTTCCATCT
TCTTCTTTGGGTATTGCTTGGGATCCACACTCTGCAGAAAAGTAGAAAAAAGAATTGTGTTAACGTCTGT
GTCCGTGTGCATGTTGCATGTATGACAGAAAGGATAAAGCTACCACGGTAGACATATTCTCAGGGCTGCT
GGAGAAGAGAATGAAAAAGAGGTTGTGGAACTTGAGGCCACATTCTTTACTGCTGAACCCCATCTTTCTA
TAATCACTGAAGGAACCTTTGTTATATATATATTTTTTCTGAATCCTACCATATGGGTGAGAATTATATT
AAAGCTCCCAAGGGTACCCAGTTTCCAGGCATTTTTGTCACAGTTCCCCCTAAATCCCTGGATAGAGTGG
CATCAGCTTGGAAATGAAGCTTTTGTTAGGAATGTCACAAGTGCCAGTACAGGTCTGAGGATCGTTGGTT
GAACCCTTTCACTGCATTCTCCAAACTGTTCTCTGTCAAAGGAGGAAAAACTGTCACCATATACAACTCT
TTCATCCTTGATGCTACGACTTCTGTTGGGGGTGATAACGGTGGCCTGACATGACAACAGCATCAAAAAG
GATTGATTTCCCATGGTGGCAGTGAGGTTTATGTGCACTTAAAAGTTATGCTAATTTTATTCTATATTTA
TGTAAATCGTCAAGAAGACCTCTAATGTTTTCTGGCGGGTCAGGCAGGTGGATGTCTGGGAAGTAAGTAA
AGGCAGAGTCTTCGGTGAGCAGTGGGGGAGGCTTCTCTACTGATGGACTCACCTCCAGCTGCAGGGTGGG
TGTGCCGTCTTTCATTACACAGGACAGGTATAGATTCTTTCCTTTGAGGCCCAAGGCCACAGGTATTTTG
TCGTTGCTTGGTTCTCCTTGTACAAAGCTCATGGAGAATATCACTGGAGAAAGGAGAGGAGTCTGGGTCA
AGGGAACAGCAATGCAGGGTGGGCTCAAGCATTAGACGTGACAGGCAAAGCCTATGCAGTCCCCCGTGTG
AACCCAGAATGAGCCTCCTCAGACACAAGATATGGACTAGCAGGGCTATACAGTCATAGTCATAGACAGT
AGCAAGGTCAGTAGGCCGATCCAGGATTCCCTCAGGACCTCTTAGGAGCAGCAATGAAGAACGAAAGGTG
TCTAACTGTTAAGCTCTAAGGGCTAAAGCTGTTATGTGGCTGGCAGGCTGTGTGTCCGTCTACGTGAGAA
GTACAGTTTGGTCGCTATGAAAACAAAGCTGTGCTCCTAAAAGGACCTAGGGGCCAGCACGTTTTGAGGA
AAATAGAGCAGGAGGCAGGTAGAACTCTATTAGCTGGGGAGAGTGGCTCCAAAAAAGAGACCCCCCCCAC
TTTGATCTTAGCCACAGTACCTCAATTGGAGGCCCCTGGCTGCTTTTATGACTTTATCAAGAAATAAGAA
TTATTCTTTTTAAAAGGTATAAAGCAATAAGATTCTTTTCACCTGGACCAGAGTAGACAGCTCCCTCCAA
AGCTTCTAAGATCAGAAGCTAAAAGGAGACAGCACACACTGCTGCTGTTAGCTCTTGTCCCCTCAGCTGT
GGTGGTGTGGTGCCAGGTCCACACCATCATCTAAAGAGGCCCTGACCGCGCTTGGAATGCTTGTTATTAT
GGAAGAGAGATGTATGCTCCTGATAGTCAGCAAACCTCCCTTCAAGGAACTGTGTGTGTGGTATTGGTGG
GAAGGATATCAGCACGAGGGATAGGACTGGGAACAAAAATGGACATATCAATTCTCTACCATCTACATTT
CAGCCTCAAATGAGGGAAAAAGAACCCACAGGCAGTTATTGCATGTCCATCAAATGACTTCACACTAATG
TGTTATCCCTATATGACTGGGCTGGAAAAATGGTCCTTTTTCGTTCTGTCTCTGTCTCTGTCTCTGTCTC
TCTCTCCTTCCTTCCTTCTTTCCTTTCCCTTTCTTTTTCCTTTTCTTTTTTTGTATAATGACCATAGTGT
TCCATTTTAAAACACAGGGACCCAGTAGGCTGCCAGGAGGCCAGAAAGGGAAACCAAGATGCTCCTACTT
ACCTTGTTGGTTGATATTCTGTCCATTGAGGTGGAGAGCTTTCAGCTCATATGGGTCCGACAGCACGAGG
CTTTTTTGTTGTTCATCTCGGAGCCTGTAGTGCAGTTGTCTAATGGGAACGTCACACACCAGCAGGTTAT
CATCATCATCCCATGAGTCACAGAGGATGGGCTCTGAAAGTTCAAGAGCAGATGTTGTTTAGGCAGATAA
TTAGAAATGCAAGCACTGTGAAGTGAAGCAGCAGAATTTGGCTTATTGAGCAACACCTAAGCATGTGCAG
TCAAGATTCTATTCTAATTTTAGTGGGGGTATAAAAACACTGGGAGTGGGCTGGGCAAGAACATTGGATT
TGGCATCGTGAGATAAGCTGCAGTGTTATAACCATTCACTAGGGACTAGCAGTAGTGGTCCTTGCATGAA
ACGATGCCAGTAATAAGACCAGCTTAACTGGGAAATTGTGAGATTCACAACCTCTAGTATAAGCCAAAGT
ACTTGGCAAGTCAATGGTTGAGTTGCAAGTGTAAGCATCATTAAAGCCTTTTTATAAGTTAATCAGCTCA
AGATAGAACTGATTTGTTTGGAGGTTTCCTCATTTTCTTCTAATGGTTGGAATGTCTAGCACTAAGGGTC
TTGCACTTGAGGGGCGTTGAGTTCTAAGAGGTGGGCTGAGAAGGACCAGAGCGAAGGTCTGCTGTCTGCT
CTCAGTCCTCATTAAGTACCTTCTTCAAAGATGAAGGAAAAGAAGGTGCTCATGTCCTCATCCTGGAAGG
TCCACGGGAAAGACACAGGTAGCTGCCACAGCTTCTCCACAGCCACAATGAGTGATACTGCCTGCCTGAA
GCTCTTGTTGATGTGCTGCTGCGAGATTTGAAGCTGGATGCTCTCATCAGGACAGCCCAGGTCAAAGGTT
TGGAAGCAGCCCTGTGGAGAAACCAGGCAGTGAGCACATCAAGTCATCAGACCAGAGGACTCCTACATCA
ACCCCAGAGAACACCAAGGGCCCGGGAGATAAGTTCTTAACCACAATCCCTGTCTATGTGGAGCCCAGAG
AATTTAATTGGCCTACCCAGGGGTACATGGCAACCAGACACAGGCTTCCTGGCATTCTCTATAGTAAACC
TCATTTCTACTTTATCTTTCTTGTACAGACCTTGCCTCACTGTATAATTAGACCTACTTAAACGTTTCAT
CTTTTGGTATCCTTGCTGCTCAAACATCATGTTATTTAATTTCTCATGACAGTATGTTCATGTTGTCTTC
TATTCAACGGAGATCACCCAACAGGCTCTCAATGGCTTCCCATACATTCTTTTTCTCATAGGAATATTTA
GCAGACACTATAAGCATAGCTTTTGTTTGTAAAATATGTCACCACTTTTCTGTTCTTTGGAGAAACACCA
CATTAAGGCCTAAACACAGTTGCCTTGAGGCATGATAACAGTTTCAAAGCTTGAAGGACAGCGATAGATC
ATTTCAATAGTGTAAAGGAAAGGTTGATTGGGAGAGAAGTTTCTGTCCAGAGTAGAGGCTCCCAGGGAAA
GCCATGTTCTGGAGCAGGCAGTGGGGGACAGAGCCTGGCTGGGAAAGTGAGAGGCGTCACCCAAGGCTGA
ACTAACTAGTTAGAGTGGTCTTAAAATACATTCATGTAAGCTAAGGACTAGATTCTGTAGCTGGGGGAGA
CTGTTGAGGGATTGGGAAGAGGTCTGAGAGAAACAGCTGCCTCCTGAGTGGGGCAGTGACGTGGCTGGAC
TGACGCCATGTGGCATTAGAGCGTTCTCAGTCATCATAGGAAGGGACAGCTGGGATGCAACATGGCTCTT
CATGAGAGCAAGTCTGGAAAGTGCCCTTGTGCTAGGATGGACTTGGCCAGCACTTAAAACACTTCAGAAT
GGAAGGTTGTGACAGATCGCCAACATTTCAGAGATCTTCTGACCTGTGTTTCCCAAAGGACACAGTGGGG
ACTGTCTATGATCACACAGCAATGAAAGGTCTTGTCTAAGGCCACAGGTTATTTTTAAAAATCCTAACTA
ACTTTTTGTTTATATCACTTATTTCTTATTATCTTAATCTCCCCAGGAACATGCGAAACTGTTCTTTGGC
AGAGAAGGAAGAGATGCTAATTCTGAAGGTCATGCTCTCTAGATCCAGAGTTCGATTTAAGTGGTCAGAT
TAAGCTCCATCTCTCTCAAGGGGGGACTAAATGTATGTTGAAGACCATACTGTCTTCTTAGCATAAAACA
CCATGATTGCCATCAGCAAGCCTTGGTGTGTGGCTGTGGTAAATGAAACCTGTGTGGGAGCCAGGTCAGT
GTGTGGGTTGCCTTATCTATGTCACTGAAAGAGGCAAAATAATCCCATCACAAAATAGCCTGGGAGCAAG
GCAGTCCCATATCCCTTCCCGTTTGGGTTTTTTTTTCCCATGCCACAGTCCCTCCACAGGTTAGGAGCCA
AGCTCAGAGTCTCACCTTCATCTTTTGGGGTCCGTCAACTTCAAAGAACAGGTCATTCTCATCACTGTAA
TGAAAACAGATGTGGTCAAGTGATAGCTCCTTATCAATACTGAGCAGACAGACTTCCATTGTTCATAAAA
CCACACCTACCATGACTCTTAAAGCTTCCCAAAGACTTCTGGGAAGTTCTACAGTGCTACGTAAGTAACA
GTCTAGAAGCAGATTCTGGGCCAGGTAAGTGGGTGCTCTGGTTGCTCTCTGTTGACTTCTGACACCTAAA
TTCTAGACTAGCAGAGTTAGCTAAAGTCTGAGCTCTCTGCCTTTGCTTCCAAGCCAGACTTTGCAAAAGA
GTCTCTTATCCTGTCCATGTGGAAGTTTTTCTTACCATTGCATCTGTAGGAGTCCCTGTAGTGACAGCAC
CTAAGTCCCTAGGTTGGGCTTGGGAGTGAAGAGGTCAGTCTTTAAGCTAGGTTGCTTGAACTCTGATAGC
CACTGAAGGAGATGAGTTGGGGACTCTCTGGCCTCTGAAAGTAGATAGCCCTCCATTCCTGAAAGCAGAG
TGCTGGATATGTGACCACTCTCCAGTACCCACTGACCTGTCAAAAGGTGGCATTTCACAGTTGAGTTCAG
GAACAGTTGCCATAGCTGCTTCAGACACCTGTGTGAAGAAAGAAAGTGGGTGGGCATCCAGCGTTAGCTC
CCCGAGGGGCGTCCTTCATATGTGTTTCTCTTAGCTCTAGTTCAGGGCAGGCATGCTAAACTGGTCTCTG
AGGAATGAAGGAACGGAGGAGCCGTTGTATAGGTCTAGAACAGTGGCCCTTTTGGAGTCAAGTGGCCCTT
TCACAGGGGTCACCTAAGACCATTGGATAACACAGATATTTACACTTCATAACAGTAGCAAAATTATAGT
TATGAAGTAGCAATGAAAATAATTTTAGGGTGTGTGGGGGGTCACTACAACATGGGGAACAGCATTAGGA
AGATGCTCTGGAAGGAAGCAACAGCAGAGCCAAACCCTAATAAAGAAGCTTTCCTACAAGACAGGAGCTG
CCTTTTAGATGTCAGCGGCTATACAGACAGTACTCATTTCCAGAGTACTTCACAAACCATGGAGGTACAG
CTACTTTTTATAAATAAAAAGCCCAAAGTCCATCAGTGGGGGGGGGGGAAAGAGAGAGAGAGAGAGAGAC
AGAGAGAGACAGAGAGAGAGACAGAGAGACAGAGAGAGACAGACAGAGAGAGACAGAGACAGACAGAGAG
AGAGAGAGATAGACAGACAGAGACAGAGAGAGAGACAGACAGAGACAGAGAGAGAGAGACAGAGAGAGAG
AGATAGAGAGAGACAGAGAGAGAGAGAGACAGAGAGAGAGAGAGACTTACTTGCACAAGGAAGCTTGGCT
GGAGAGGATCCCAGATGAGCCTATTAGGCCTC

Protein Sequence : Show Sequence

>gi|6680415|ref|NP_032387.1| interleukin-1 beta precursor [Mus musculus]
MATVPELNCEMPPFDSDENDLFFEVDGPQKMKGCFQTFDLGCPDESIQLQISQQHINKSFRQAVSLIVAV
EKLWQLPVSFPWTFQDEDMSTFFSFIFEEEPILCDSWDDDDNLLVCDVPIRQLHYRLRDEQQKSLVLSDP
YELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKK
KMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS

Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis galU mutant vaccine , Francisella tularensis guaB mutant vaccine

10. IL-6

Gene Name : IL-6
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16193
NCBI Protein GI : 13624311
Genbank Accession : AC112933
Protein Accession : NP_112445
Taxonomy ID : 10090
Chromosome No : 5
Gene Starting Position : 30013160
Gene Ending Position : 30019967
Gene Strand (Orientation) : +
Protein Name : interleukin 6
Protein pI : 7.5
Protein Weight : 23291.86
Protein Length : 211
Protein Note : Also known as Il-6

DNA Sequence : Show Sequence

>gi|372099105:30013160-30019967 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
ACCAAGAACGATAGTCAATTCCAGAAACCGCTATGAAGTTCCTCTCTGCAAGTAAGTGAAGGCAGTTCCT
TGCCCTCTGGCGGAGCTATTGAGACTGTGAGAGAGGAGTGTGAGGCAGAGAGCCAGCATTGTGGGTTGGC
CAGCAGCCATCAGCTAGCAGCAGGCGCCCAACTGTGCTATCTGCTCACTTGCCGGTTTTCCCTTTTCTCC
ACGCAGGAGACTTCCATCCAGTTGCCTTCTTGGGACTGATGCTGGTGACAACCACGGCCTTCCCTACTTC
ACAAGTCCGGAGAGGAGACTTCACAGAGGATACCACTCCCAACAGACCTGTCTATACCACTTCACAAGTC
GGAGGCTTAATTACACATGTTCTCTGGGAAATCGTGGAAATGAGAAAAGAGGTGGGTAGGCTGTGAAACT
GATGAAGACCCAGTGTGGGCGTCCATTCATTCTCTTTGCTCTTGAATTAGAAATTCTCTGCTGGGATCTA
GGGCCCTTAGGATTTGAAGCTAAAGGTCAGACTAGACTGTGTTCTCTCTTTCTCACCTCTTTGCTGGTTT
TGAGTGGAGGTTGGGAAGGGGTTCCTTTCCTGTCTGGAAGATACAGAATGTGACTGCATTTCTAGAAAAT
TCACAGTGGGCCATTCTCTGTTCCAATTTAGCTATTGCTTCTTAGGTGGGGATTCCTTTTCCCTCATTAT
TCCTTTTTCCTTATCTCTTTGCCTTCATTTATCCCTTGAAGTCTACAAGGACCAGAAAGTGCTTTTTGGC
TGAATGTAGTAAGTTCTGTGTGTGTGAAATCAGGATGCTCTAGGGTCAGCCCAGATAAGATGCATATTGT
ATAGAGCCCAATAAAGTGCTAACACACACACACACACACACACACACACACAACCTAGATTAGGCTGGGC
ATGGTGGTGGATGCCTCCCAGCACTTGGGAGGCACACGCAGGAAGATCTCTGTGAGTTAGAGGCCAGCTT
GGTCTACATATTGAGTTCCAGGATATCCAAGACTACATTGAGAGATCCTTTCTCAAAAAAAAAAAAAAAA
AAAAAAAAACAACAACAACAAACAAACAAACAAACAAGAAAAAAAAAACCCAACCAACCCTACCTAGATT
AAAATATCTCCCATCTTTAAATGAAAAGTATTTTGACTCTGCTTGACAGAAAGGAAAAAAAAAATCACAA
ACAAGATTAATAGCCTAGGTCTGGGTGTGTAGAACCAACTTTGTAATCTTGGGAAAATCCTCAACTATTT
AGTACTCTTTATCTGCATAATTATGGAGTTACAGATAATGGCACAGACCCTTCCAGATGGAATATTCTGT
GACTTAAATTTATTTGCCCCAAGATATCATCAAAATTTCTGTCCTTTACCAAGTTCCTACTCTATGCTGG
GCAGTTTTTATCGTTGAATCCTTGAGACAAATGTATAAGAGGGACATCAATAATTCTTTATTGAAACATA
GGAAATACTTAGGAGAGTAACAAGCTTAAAGCCAAGGGACTGAGCCTAAGGGTGCATTTTTATTTATTGG
GCTGCTTAGTAGGGTTCTAAGAAGGTATAGCTTCCCTTTTCTGTGTCCTGGCTATGAAAGAGCATGACTT
GGCCCTCTAGTGGTGCTTGTTGTAAGAGGTGTTTAAGTGATTGCTCTGGTAATCTTTTGCAGTTGTGCAA
TGGCAATTCTGATTGTATGAACAACGATGATGCACTTGCAGAAAACAATCTGAAACTTCCAGAGATACAA
AGAAATGATGGATGCTACCAAACTGGATATAATCAGGTAGAAACTTGTCACGTTTACTTTCAACAATTCT
TCAAGGTTCTCTCTTGCACGTGATACCTGCATTTGTACAGTCCCAGTCAGGCAACAAAGATGGGGGGATA
CATCCAATACCACTTGGATGTAACTGGCCTGCAGTAGCTTGAATACAAAAGGAGATTGAGAGGTACCTCA
GCAGCTGCTAGGGTGAGGGAAAGTTTACCAACCAGTGCTATAAATGTTCACTCAATCTATGTCCATCAGG
AGCTAAATGTTTAAAGTCTCTATTCTCATCTTGGCTTTGAGGTGTTAGATGATTAAAAAAAAAACATTTT
TTTTGTCTAAAAAAGTGGAAGAGTAGATATAAAACATGTTATGTAAACGTCATGAGGAAAGTAACTTACA
GATTTAATTAAAAAGCAGTTTATTTAAACAGGTAAAGGCCCACTATGCTGGGATTCTTCACCACTGTTAA
ATGTTAGAAGTCATAGCAAGTTTAGCTAGTTCATGAGCCATCTCTTCTCTGGCTGCCCCTGGAAGGATCC
AGGCCTACACTCCCTACTGAGAACACTCTTCACACCCCTCTCCTTCCAGCCGGTGAGATAGTCTACATCA
GGTCTCAGGACAAGTCAGACAAGAACAAGAGTACAGGAAGGGCACTGCAGGATAGAAGGGAAGGAGTGAG
GGTCAGAAGTGGATTCCCACTGCCACACTCTGGGGTAGGGGGTGGGGGGATATTAAGTGGTGCCTCTCTC
TACTTGTCCAGATAATTCAGGAGGTCAGCTGATGCTGCCTGTTGTCTAATTCTGTAGAAGATACTGAACA
GCAGGAATCTTTTTTTCAATTTTTTATTAGATATTTTCTTCATTTACATTTCAAATGCTATCCCCACAGT
CCCCTATACCCTCCCCCCCCTCCCCGCTCCCTTACCCACCAACTCCCACTTCTTGGCCCTGGCATTCCCC
TGTACTGGGGCATATAAAGTTTGCAAGACCAAGGGGCCTCTATTCCCAATGATGGCTGACTAGGCCATCT
TCTGCTACATATGCAGCTAGAGATATGAGCTCTGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTAT
AGGGTTGCAGACCCCTTCAGCTCCTTGGGTACTTTCTCTAGCTCCTCCATTGGGGGCCCTGTGTTCCATC
CAATAGAAGACTGTGAGCATCCACTTTTTGCCAGGCATTGGCCTAGCCTCACACGAGACAGCTATATCAG
GGTCCTTTCAGCAAAATCTTGCTGGTATATGCAATACTGTCTGGGTTTGGTGGCTGATTATGGGATGGAT
CCCTAGGTGGGGTAGTCTCTGGATCTTCCATCCTTTTGTCTTAGCTCCAAACTTTGTCTCTGTAACTCCT
TCCATGGGTATTTTGTTCCCCATTCTAAGGAGGAATGAAGTATCCACATGTTGGTCTTCCTTCTTCTTGA
TTTTCTTGTGTTTTGGAAATTGTACCTTGGGTATTCTAAGTTTCTGGGCTAATATCCACTTATCAGTGAG
TGCATATCAAGTGACTTCTTTTGTGATTGGGTTACCTCACTAAGGATCATACTCTCCAGATACATCCATT
TGACCAAGAATTTCATAAATCCATTGTTTTTAATAGCTGAATAGTACTCCATTTGTAAATGTACCACATT
TTCTGTATCCATTCCTCTGTTGAGGGACATCTGGGTTCTTTCCAGCTTCTGGCTATTATGAATAAGGCTG
CTATGAACATAGTGGAGCATATGTCCTTATTACCAGTTGGAACATCTTCTGCGTATATGCCCAGAAGAGG
TATTGCTGGATCTTCCGGTAGTACTATGTCTAATTTTTTGAGGAACAGCCAGACTGATTTCCAGAGTGGT
TATACAAGCTTGCAATCCCAACAGCAATGGAGGAGTGTTCCTCTTTCTCCACATCCTTACCAGCATCTGC
TGTCACCTGAATTTTTGATCTTAGCCATTCTGACTGGTGTGAGGTGGAATCTCAGGGTTGTTTTGATTTG
CATTTCCCTGATGATTAAGGATGTTGAACATTTTTTCAAGTGCTTCTCAGCCATTCAGTATTCCTCAGTT
GAGAATTCTTTGTTTAGCTTTGTACCCCATTTTTAATGGGGTTATTTGAATTTCTGGAGTTCAGCTTCTT
GAGCTCTTTGTATATATTGGATATTAGTCCCCTATCAGATTTAGGATTGGTAAAAATCCTTTCCCAATCT
GTTGGTGGCCTTTTTGTCTTATTGACAGTGTCTTTTCCCTTACAGAAGCTTTGCAATTTTATGAGGTCCC
ATTTGTCGATTCTCGATTTTACTGTACAAGCCATTGCTGTTCTGTTCAGGAATTTTTCCCCTGTTCCCAT
ATCTACGAGGGTTTTTTTCCCACTTTCTCCTCTATAAATTTCAGTGTCTCTGGTTTTATGTGGAGTTCTT
TGATCCACTTAGACTTGAGCTTTGTACAAGGAGATAAGAATGGATCAATTCGGGCTGGAGAGATGGCTCA
GTGGTTAAGAGCACTGACTGCTCTTTCAGAAGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGTTCAT
AACCATCTATAATAAGATCTGATGTCCTTTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATGTA
ATAAATAAATAAAACTAGATTATTTAAAAAAAAAAAAAAAGAATGGATCAATTCACACTCTTCTACATGA
TAACCACCAGTTGAGCCCGCACCAATTGTTGAAAATGCTCTCTTTTTCCCACTGGATGGTTTTAGCTCCC
TTGTCAAAGATCAAGTGACCATAAGTGTGTGGGTTCATTTCTGTGTCTTCAATTGTGTTCCATTGATCTA
CCTGTCTGTTGCTGTATCAATACCATGCAGTTTTTTTTATCACAATTGCTCTGTAGTACAGCTTGAGGTC
AGGCATGGTGAGTCCACCAGAGGTTCTTTTATTGTTGAGAATAGTTTTTGCTATCCTAGTTTTTTTTTTT
TTTTTTTTTTGAACAGCAGGAATCTTATTCTCAAATTGAATCTATTCCTAGAAAGAACTGACTTCCTTTT
CCATTTTACTTATAGGAAATTTGCCTATTGAAAATTTCCTCTGGTCTTCTGGAGTACCATAGCTACCTGG
AGTACATGAAGAACAACTTAAAAGATAACAAGAAAGACAAAGCCAGAGTCCTTCAGAGAGATACAGAAAC
TCTAATTCATATCTTCAACCAAGAGGTGAGTGCTTCCCCATCTCTCATGCAGTGTGGGAAAGAGGACACC
CGGCACCTCAGGGTAGCGGCACTTTTTCCAGACAGCTGCTCAGAAGGGAGAGAGTCTGAACAACAGTTCT
TGATTATAAGGCCTTGCTTTGTTTTACTTTGGGGTTTTGTTTGAGGTTCTCTTTTGCAAAGAACATCAAT
ACCTGCTTTAAACTGTATTAATAGAATGTTACTAATTGCGTAAGAGGTATGAAAACTATGACAGCCATAC
ATAGTCACCCATTATGAGAGCACAGAGACAAAAGTGACTTTAATATTTAATCCTTGGCAAGTGACATTTT
TGTAACCAGAGTTCTAATGCAGAGAAGTAGCCAAAAGCTAAAATGTCAGGGAAGGAAGGGATCAAGGGCT
TCTGGCTACCATTAGCCAGAAGAATGGTAGATATGGAGGATTAATGACTCAGAGTGTGGGCGAACAAAGC
CAGATGCAATAAGAAGGGCCTGGAATGAAACCCTCTTGCTAAGGCTGCTTTTGCCACTTGTAGTTTCTTG
CCTTAAACCAGAGAGTTTCCCAATTTAATGTGCACAGGAACCACTTAGGGTCTTGTTCCAGCAGGGTCTT
ACTTAGGAGGTCTAGGAAGGGAACTAAGATTCATTTTTGTAAACAGCTGCTTGTTTATGACCCTGCTATA
TAGTATTCAGGCTCCTAAGTAGTGGGGCAAGCCTTCCAGTTAGTCTTCCCCATGCCAGTGGGCCCATGGA
AGGGTGTTTCCAGACTTCTTCATGCTACCACACTAAGAGACTCTCAACAGAGTCTGAATGGAAACCACGA
AGGAACACATTTGTTTTAGATTCCTCTGTGCCACCTTTACTGATGGGAGCTTCTGTTTTCCAGTAGAATA
CAGTATGTTAGCATGGATGCTTGGATAACAGACAAATAAGATGGTGGTGCTGACCTCTGGACGCTTACTC
TCTAGTGGCAGACAGAACAGTAAGGTTAGAATTCTGTTGCTATTAAAAAACTAATAATAATCACCTTGAA
AAAGAATGGAGTTGTTAGGCATGGGTCTCTCTCGAGTAAGCTTGGAACAAAAGCATCTCCCCTGGCTTGG
GTGAGTCAAAGCAGATGGACTTAGCTCGTCTCATTCATTCTAAATTAGAACTTCTTCCCACAGCCCAGAA
CACGCCACAAGAAAAAAAAAATGTGCAATATTTAACCAGTCTTTGTTTTTTCCTCCTTTAGGTAAAAGAT
TTACATAAAATAGTCCTTCCTACCCCAATTTCCAATGCTCTCCTAACAGATAAGCTGGAGTCACAGAAGG
AGTGGCTAAGGACCAAGACCATCCAATTCATCTTGAAATCACTTGAAGAATTTCTAAAAGTCACTTTGAG
ATCTACTCGGCAAACCTAGTGCGTTATGCCTAAGCATATCAGTTTGTGGACATTCCTCACTGTGGTCAGA
AAATATATCCTGTTGTCAGGTATCTGACTTATGTTGTTCTCTACGAAGAACTGACAATATGAATGTTGGG
ACACTATTTTAATTATTTTTAATTTATTGATAATTTAAATAAGTAAACTTTAAGTTAATTTATGATTGAT
ATTTATTATTTTTATGAAGTGTCACTTGAAATGTTATATGTTATAGTTTTGAAATGATAACCTAAAAATC
TATTTGATATAAATATTCTGTTACCTAGCCAGATGGTTTCTTGGAATGTATAAGTTTACCTCAATGAATT
GCTAATTTAAATATGTTTTTAAAGAAATCTTTGTGATGTATTTTTATAATGTTTAGACTGTCTTCAAACA
AATAAATTATATTATATT

Protein Sequence : Show Sequence

>gi|13624311|ref|NP_112445.1| interleukin-6 precursor [Mus musculus]
MKFLSARDFHPVAFLGLMLVTTTAFPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELC
NGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKAR
VLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQ
T

Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis FTL0552 mutant vaccine

11. Il12a

Gene Name : Il12a
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16159
NCBI Protein GI : 226874945
Genbank Accession : AC121306
Protein Accession : NP_001152896
Taxonomy ID : 10090
Chromosome No : 3
Gene Starting Position : 68690643
Gene Ending Position : 68698546
Gene Strand (Orientation) : +
Protein Name : transcript variant 1
Protein pI : 8.33
Protein Weight : 25111.9
Protein Length : 236
Protein Note : Also known as p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232

DNA Sequence : Show Sequence

>gi|372099107:68690643-68698546 Mus musculus strain C57BL/6J chromosome 3, GRCm38 C57BL/6J
CTGCCACCTACTCCCTTGGATCTGAGCTGGACCCTTGCATCTGGCGTCTACACTGCTGCTGAAATCTTCT
CACCGTGCACATCCAAGGATATCTCTATGGTAAAAGTCCAGAAAGGCTAAAGATGTTGTCCACAATCTCT
CGGCAATGACCAGATATATCCCCACCAGTATCACAACTTGCAACGCGCTCCGTTCCCATCTGCCCTCTGC
CTCCCTACTGTGTTGATTCCCATTTCCAAAACTAGCTCAGAATGCATCCCACTGGCAGCCAGGCAATGCC
TTCTTGCAGATCTGGAGGCTTCAGGAGCCTTTGCTGCCTCTTAGGCTCCTGCAGAGAGATGGCCTTGCTC
TCTCTGGGCCCTTTTCCCCCAAGATGCTGTGCAGTCGTCTCACTAAATCAAAACTGCCAGAACTGAGTTT
TAAGCGATATATAGCCACCACATCTATTTTGTTCCTTTATACTTTCAAAGTAGGACAGATTCGCAAAATA
CATTGAGTCAGACTCAGTGTCCACGATGGAGAGACGAGTGGGTAGCTTGTGCTTCTTTAGAAGGGGCACT
GGGCAGCGTGTTAGGGCTAGGGGGTAGTGGGGCAGGACAGACCTGTCCCGGGACAAGAGTGGCTACTCGC
AGGCCGGTTTTGCTTTCGCTCTGAGTGTGGTGGGCGGTGGGACGGTCCCGAATCTCCTCAAACGGGCACT
CCGAGAGTTAATTCGAAAGCGCCACCAGCCTTGGGGGCGATCGACGCACTTGTCCTTGAGATGTAGAGAC
AGAGAGAGAAAGCAAGAGACACAGTCCTGGGAAAGTCCTGCCGGCTATCCAGACAATTATAAAAATGTGT
CTCCCAAGGTCAGCGTTCCAACAGCCTCACCCTCGGCATCCAGCAGCTCCTCTCAGTGCCGGTCCAGCAT
GTGTCAATCACGCTGTAAGTACCTGAGGGCCGGGACCAGGGTGACTTGATGAGAGGAGAAGGCTTGAGAA
AGAACAGACAGAAGCCCTGGACCCATTAAGGGAGACTAAAGGGCTGCCAGCAACTGCAGGCCAAGGGAAG
GAAGAGGGGAAAGATGGTCGTGCTAGATTGCGCTGCACCGAGCATGGTCTCATTATAGAACAACCCTGGG
GGCTACGGCTATGCCCGTTTCACAGAAGAGAACTAAAGCCCAGCCTTGAAACCAATGTAAGGAAAATGCA
GGTCTCAGGCAGTCCCCAAACAAGATCTGGGAGGTCTTGCTTCATCTGTCAGCCCCGGGGAAGGGCTTAA
ATTATGTGTGGAGGGAGGTCAGTACCCAGAATGGATGGAAAAAATTGCCAGAATGGCTGAAGAAAGAGGC
AGGTCAAAACCAAGTTGACAAGCTCCACCTGCCCTTTGTTTCCCTTCCAGACCTCCTCTTTTTGGCCACC
CTTGCCCTCCTAAACCACCTCAGTTTGGCCAGGGTCATTCCAGTCTCTGGACCTGCCAGGTGTCTTAGCC
AGTCCCGAAACCTGCTGAAGACCACAGATGACATGGTGAAGACGGTGAGTTTCTCCTGTCCTTCTGGCTT
CAAAGTTCCGCTGTTGACATGACTTCTAACCTATCTGGTATCTGCTGGGAAGTTTATGGTATCTGGCAGG
AACCGTAGAGGGTTTCAAGACTTCTAAATATAATCTAAAGGAAACTGTGTCTCTAGAGAAAGGAAGAATG
GTAATAAATTGTAACGGCATCGGTTTAGGCTGTGTCTACACAGAGGAAAGTGGGGACTTGCAATGTCCCT
CCCAGCCTGCCTGCCTGTGTGACACAGTGTCAGGTGCTCCCTGCTTCTGCTTCAGGGCAGGTGGAACAGG
GAGACAGTCTCTAGGTACACTCTCTGTGCTTCACAAGCCATAGAGATGAATTAGATTGTCTCTCCCCCTG
CTCACCAGTGTCCAGCACTGTGATGCAGTCCCTTGTCGGATGGACACATGTCAGGGAGGACTCAGACATC
AGATAGCAAGCAGGTCCAGCCTCCAGTTTTCTGGGGCAAGTGTGATCCAGTTTTAGTCACGGCTCCACTC
ATTTTTCTCAGGTTTCATGTTTGAATGCAAAATAGCCTTTTCCGTTGTAAAATTGCCACGGTTTCCTCTC
GCTTTGGGGACAAAGCTACAGCAAAATGCTAATGTGTTTGAGATATCCTGCAGCTGCTAGGGCTGGAGAT
CCAGTAGAATTCTGGCTTAATTATGAATGGGTCTCCCGGGTTCTATCCCCCAACAGAGCGTGCGTTCATA
AGCACCTGGGAACAGAGGAGGGAAGGAGGGAAACTCTGTCTTCCTGTTTTGTTTTTAAATCTTTCTTGAG
GATCTTCTAGCTTGTTGTCTTATGGCTATCCCAACATAAGCTCCGCTCCACAAGCCCTTCTTGGTGAATT
TTCTTTCTTTCCTTCCCGTCTTGCATAACGTGCTGCTGGCTGTTCCTCCAAGCTCAGTCTTAATTGAGTG
GGGTTCCCAGGAGGGAGAGATGGCTCTGTGGTTAAGAGAGCTGGCTGCTCTTCCAGAGGTCCAGAGTTTC
CACACCCTCATGGTGGCTCACAATCATGGTAACTCTGATCATAGGGAACCTGTCGTTTTCTCTGTGCCTC
TGTGGGCACTGAGCACATGCCACACAGACATCCATGCAGGCAGAACACTCTTGGCCATGAAACCTTAAAA
TGAAGGAGAAACCTTACGAGAGGTTCCCTAAGGAATCCCTGCAGAAGGGAGAGATTGTTTTCCTAAGATG
AGGTTTGGTGTGGAGGGGATGAGGTATTCAAAGTTAACTAGCCGCTCCTCACTCCTCTGCTGCCAAATAC
CAGCACTGGGCTTCGAGACACAATAGAATTCGACCTTGTTTTCGTTTGGGTCACTATTGCTCTCTAGTTG
AACACGGAATCCACGGGGGCACATTGCTGTCTGTCCTTTGTCACACCCCAGGACCACAGCTGCATGGAAA
AGTCATCGCAGGCTTCAGTCAGAGAGCTGTTGACTTCCTAATATGTTGTGTAATTCTGTTCAGTGGTGCT
TTCCAGCACACCAGGGAATAAGAGACAGTCCCCGCGTATGTAAATTAAAGCTCAACTAGTATTTAGGGCT
TGCAAGTTTGTTACAGCCATCAAGCTGTGACTTTAACAGTTTCCTCTTTAGAAGTTAAACTTGAAAAAAA
ATAGAGGGAGAGAGAGATTCACAGGGCACAGCTGCTTGGAAAAAGGAAAAGCCACAGGACTCACCAGAAG
CAAGCAGCACAGTGTGGCACTGATGCTGATGAGTACAGCCTGCTGCACCCCAGTGTTCACATGGTGAAAA
AAATACCCTGCTGGCGGACAGCCTAGGCAAAGGGGGAGGAGAGTAGGGAGAGCGTGCGGGAGGTTGGGTT
TCTTAGCTTATGAAGAGCCTGAGTTTCCTACAGGCCAAAGAGATGACGGTTCAGAGTGGCCAGCCTAGGC
TCTGCTGATCCACTGCGATGCAGGTCTCAGAGGGAACCAGCTGTCTTGCTGAGAGATTCACCTCAGCTTC
TCCTTATGTTTTTCCCAGGCCAGAGAAAAACTGAAACATTATTCCTGCACTGCTGAAGACATCGATCATG
AAGACATCACACGGGACCAAACCAGCACATTGAAGACCTGTTTACCACTGGAACTACACAAGGTATGGAG
GACCCCCACCCAAAAAACAACAACAACAACAACAAAAAACACAATTAAGAGCCGTGCAGCCAACCTCAGC
TGTCTCCAGATACTTACTTGTAGCTATCAAACGTGAGAGTTTTGAAGAAAAAAAAAAAAGTCAACAACCA
TACAAATACTTCTACAAGCTGAAGGATATACTCGGTAACTAGGAGGTGGCTATGGTGGTATAGACCTTTA
ATTCCAGCACTTGATGGATAGAGGAAGGCCGGCTCCTGTTAAGTTAGAGGCCAGTCAGGGATACACAGCA
AGATTTCATCTTTAAAAAGAAAGGGGGTGGTGTGGCGGGTGAAGCTTACTTATTGAAGTGCTCATGTCAC
AAGCCTGGGGACCTAAGTTCCCATCCCCAGCACCCATGTAGGAGCTGGGTGTGATGGTTTCACATGCAGA
GACAGGAGGATACCTGGGCCAGCTAGTCTAGCCGAATCCATGAGCTGCAGGCCCAGTGAGAGAACCTGTA
TCAGAAAACAAGAAAGTCAGGAGAGCGGCTGAGAAAGACCCCAAAGATCATCCTCCGATTTCCACGAGTA
CATGCACCTGCACACACGTGCACACAGGAATCCTATACATAGCCACCCAAAAGCAGTTATGATGGTGAAG
TTTCTTTTACTGGAATGAAAAGTTAGTTTGAGAAAAAAATATCAGAGAGATTTATATTAAGAGTGAATGG
GTGGGGGGTATGCATGCCTTTAATCTCAGCACTTGATAGGCAGAGGCAGATGGATCTCTGTGAGTTCAAG
GCCGGCCTGGTCTACAGAGTAAGTTCCAGGACAGCCAGTGCTACAATAGAAACAGGTGATGGTGATGATG
GTGATGATGGCGGTGATGATGATGATGATGACGAAGACAAACTCCTCGGTTTATGATTTTCTTCTAGAAC
GAGAGTTGCCTGGCTACTAGAGAGACTTCTTCCACAACAGTAAGTAAGCACTCTAAGGGTTCCTTCCCCA
TGACGGATTCATAACACTGATGCCTGGTCATTCTTTCTCTAGAGAGGGAGCTGCCTGCCCCCACAGAAGA
CGTCTTTGATGATGGTAAGCCACATCACTTTTCTGAAATGCAGCAGGGTCCTGTAGTTCCAGCCCATCTC
AATGATTCCTTGCCCATTGTGTCCTGCGCTCTAGACCCTGTGCCTTGGTAGCATCTATGAGGACTTGAAG
ATGTACCAGACAGAGTTCCAGGCCATCAACGCAGCACTTCAGAATCACAACCATCAGCAGATCATTCTAG
ACAAGGGCATGCTGGTGGCCATCGATGAGCTGATGCAGGTAAGCCTTCCTCCTATCAGCCAGCGCTCCTT
TTCCATGGTGAAGAGAGGTCCTTTATTAGATATGATAAAATCAGCACAGCTTGGGTTGGGAGAGACCTGA
ACACCCCCACCCCACCCCACACCCTCATCCCCCAACCATGAAAGGCCTTGCAAGGTTACATTTGCTTAGA
GAGCCTTGAGGGGGCCTCTACAATAAGGGCAAATGCAAGCAGCCTTCTACTTGTTGACTCATGATACAAA
TCAGGCACTTTTCAAATGCTTCTTCTCATACCATCCCCACAACAAGCAAATAAGGTCTCTACAGAGGAGG
AAACTGAAGCTTAAGAGGATGAACGAGGGTTCCCTACAAAAGGGTTTTTTGTTGTTTGGTTTGGTTTGGT
TTGGTTTGGTTTGGTTTGGTTTTTCCGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTCAC
TTTGTAGATCAGGCTGGCCTCGAACTCAGAACTCCACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGG
TGTGCGCCACCATTGCCCAGCTTATTGTTGTTTTAAATACATTCTTTTCTTAAAGGTTCAAGATTAGGAC
TGGAGAGACAGTCCGATGATTAAGAGGACTTGAGTTTGGCCTGGGGCTCCTATGTAACAAACAGGGTGTG
GTCCTGAACTGCTCTCTAACTCCAGAGAGTGGAGACAGGAGGCCTATGGAGGCTTTCTTGCTGCAAGTTT
AGCCATGAAATCTCCAGGTTCAGGGAGAAAGCATGCCTCAAAGAAATAAGATGGAGAGTCACGTGATCTC
TTCTCTGGCTTCTGCATGGTGTAGGCACAGATATGCACATGTTTGAGTGCATACAATACAGGCAAACATA
CACGTGTCCTTTTTAAAATGTATAGGATGGACATTCTAGGGGCTGCACACATATTCAAATATTACTAGGG
GAAGCACTTGGGGCTGGTGTCCAAGTATAATCTATTTTGAACCAAAATGTGACCAAAATGAAAAGTGGTC
TTACATCTAGGTAATTACAGTGTTAGGTATCCGTGGGAACTTTGCTGTACCACCAGTAACACAAAGCACA
GGAGTGTGCACACCAGAGACAAGCACCTTGGCAACCAAGGCGTGGGATGAACATATGTTCAGAAGTCACT
GCCTGGAAAGCAATTGTTTTTCATTTCATACATACTGTCTTGCACAGGGTTTCTATGTCTTAGATAAAAT
ATCATGACGGAAAGCAAGTTGGGGAGGAAAGGGTTTATTTCATCTTGTAGAGTGTAGTGAGTCTATTACC
CAGGGAAGTGGAGGCAGAAGCTGATGCTGGAGAGGACTCCTGGTGACTGGAGTGCTCCACATGACTTGCT
CAGTGTGCTTTGTATAGCACCCAGGACCACCAGGCTAGGGTGAGCTGGTGCCTCCCACATCAACTGTCAA
TCAAGAAAATCACCAGAGGCTTGCCCACTGGCCAATCTGGCTGGAGGTGTTTTTCTCAATGACCACAGGC
TTGTGTAGAGTTAACATAAAAGCACCCAGGACATTACATATTGCCAGATGACTCCTTTGAAAGACAGTGA
AGTTTATGCAGGTCAAACAACCTATCATGTAGAGATTTGTAATGGATATTGCATGAACAAGGCCAGAAGT
CAGGTGATTTGGATAGTAGGAAGAGGTCTACCAGGGACCAGCCAACTATGCTGACAGGGTTAACTCTTGT
CAGCCTGTTTGTAAAATAAGATGACTTTAAATAGCCTATGTCTCTCTTGAGATTTTTGTGAGGAGCAAGC
AAAAATCTTAAACATCACAGAAACCTAAGTTCTTATTCTATGTTATGAAATCAAAGGATCGAGTGGGGAA
ACACGGTACTGGCTTGTTTATCTACCACAAATAGCGTTTCTATCAGAGATAGGTCTGTTGTTGATAATAA
GCTCTAATCCATAGATCCTACTTACCCTCCAACTCCCTCCTCTAAAAGAACAGGACTTCTTAACATAATT
TATAAAAATTAATTAGCTATTGCTGCAGGTGCTACCTCATAGACTCACACCTTCTGAGAGATCAGCTCTA
ATGACAATTTCTTTCTGGAAAATTTAGGGGTCTTGTCAGAGATCATTAAAAACATAGGTATATGCTCACA
AAAGAGTATTTCTTCCCTCTTTTCCTGCAGGAACACAGCAGGAGTAGATGTGAAATGGGTCTAGGAGTGT
GATGAGGGGCTAGGTTGCAGGCCCTGGACTTTGCATTGACTGTCTCCCATTTTGCAGACAAACATTCTAA
TTTTTAAATTGTGCATATGCTATGCTTGGTGTTTCTTTCTAGTCTCTGAATCATAATGGCGAGACTCTGC
GCCAGAAACCTCCTGTGGGAGAAGCAGACCCTTACAGAGTGAAAATGAAGCTCTGCATCCTGCTTCACGC
CTTCAGCACCCGCGTCGTGACCATCAACAGGGTGATGGGCTATCTGAGCTCCGCCTGAAAGGCTCAAGGC
CCTCTGCCACAGCGCCCTCCTCACACAGATAGGAAACAAAGAAAGATTCATAAGAGTCAGGTGGTCTTGG
CCTGGTGGGCCTTAAGCTCCTTCAGGAATCTGTTCTCCCATCACATCTCATCTCCCCAAAGGTGGCACAG
CTACCTCAGCATGGTCCCCTCCATCGCTTCTCTCATATTCACTATACAAGTTGTTTGTAAGTTTTCATCA
AAATATTGTTAAGGGGCGAAGACGTCCTCCCCTCAATGTGTTAGCAGAAGAGCAAGAACTGATAAGCTAT
TGTTTTTGTGCCAAAGTGTTTATGAAAACACTCAGTCACCCCTTATTTAAAAATATTTATTGCTATATTT
TATACTCATGAAAGTACATGAGCCTATTTATATTTATTTATTTTCTATTTATTATAATATTTCTTATCAG
ATGAATTTGAAACATTTTGAAACATACCTTATTTTGTGGTTCTAATAAAGTAATGTTATCACTT

Protein Sequence : Show Sequence

>gi|226874945|ref|NP_001152896.1| interleukin-12 subunit alpha isoform 1 [Mus musculus]
MVSVPTASPSASSSSSQCRSSMCQSRYLLFLATLALLNHLSLARVIPVSGPARCLSQSRNLLKTTDDMVK
TAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLC
LGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKM
KLCILLHAFSTRVVTINRVMGYLSSA

Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis guaB mutant vaccine , KKF24

12. Il12b

Gene Name : Il12b
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16160
NCBI Protein GI : 6680397
Locus Tag : RP23-388G23.1
Genbank Accession : AL607030
Protein Accession : NP_032378
Taxonomy ID : 10090
Chromosome No : 11
Gene Starting Position : 44400062
Gene Ending Position : 44414016
Gene Strand (Orientation) : +
Protein Name : interleukin 12b
Protein pI : 6.5
Protein Weight : 35952.67
Protein Length : 335
Protein Note : Also known as p40; Il-12b; Il12p40; Il-12p40

DNA Sequence : Show Sequence

>gi|372099099:44400062-44414016 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
CAGAAGGAACAGTGGGTGTCCAGGCACATCAGACCAGGCAGCTCGCAGCAAAGCAAGGTAAGTTCTCTCC
TCTTCCCTGTCGCTAACTCCCTGCATCTAGAGGCTGTCCAGATTCAGACTCCAGGGGACAGGCTACCCCT
GAACCAGGCAGCGTGGGAGTGGGGTAAGTGGATTCTGGGAGCATCTCGGATGGCTTTCCCCGCTGGTGGA
AACCAGGGGCTTTGACGACAGGGGCTTCAAATAGTCATTTAAAAAGTCAGCTAATTACAGATGTGATGGC
GCACACCTGTAATCTCCAAATCTGGGAGGCAGGAAACAGAATGCACAGGAGTTTAAGGCCAGATTCAGCT
GTGTAATGAGTTTGAGAGAGTCTCAAAAAATTGAGCTACTAATAATATGTGCCAAGCATTGTGGACAGAT
GTGTATTTGATAGAAAGGTTTGCTTTGTCTATATAGACACATATACTATGTGCATTTTCTTAAGTATTAA
TGCATATGTGTTAAGGAGTGTTGTTAAAATTCTGTTCTTGCCTTAAGAGTGAAACTTAAAAAAAAAAAAA
GCCTTTCATGTACCCCTTCAAGCCTAAGCTCATCACTGAATGTCCTACCTGTGATGGCCTTTAGAATCAG
CTATGATAATCAAAGTGGAGAGCTGGGTTTGGGGGTTCCTTTGGATTTTAACTAACTTTGCCACCTCTTC
AAAACTGGAAAGCCCAAGGCATGTTCCAAACTATTTCCTAAAGGGTTCTAAAATCTGTGAGAAAGGGAAG
GCTAATCTTCATGGGTTGGCTTCCTCTGCTTGCCCAGGTGAAATCATGAGCACCAGATGGGGCAGGGTGA
TGGGGCAGGGTGACATCTTGCTTTCACCCAGGGATCACTGAGCCTCAGCTGGGGGCTCCGTTTGTTCAAG
CAAGGCATTTCAAGATGAAGTTAGGTCACCCCCAAGAATTCATTTTGCTGAGATCTGAAATACTGTGATT
ATTGCACCAACAATCCATGGACTCTCTCTAGGCTAGATTCTGGATATAAGACTAGACAGATATGACCTCT
GTCACACACCTCGGTATGGGAAAGGGGGGACAGACATTAAAGACGTAATCAAATGATTGTCATTTCAGCG
ATGCTGAAGGGGAAGAGTAGCTATTTGGGGTCCTGTTTTCATTTGGGGCTCAAGCTGGCACTTTCTGTGG
ATCATTTCATGAACTCCTAACCTGACAGAGTTATTTTTAATCTAGGAGTACAGGCTTGACAAAGTCAACA
CAACTTGTCAAGGTCACATAACTAACAAGTGGCAGACTGAGGTCTGTTTGGCTGTAGAACCAAGGCCCTT
TGCCCGTGCCCTCCTTGCCATCCCTGACAGCCACGCCATCTGTACTGTGCATTGTTGTTTCTGGGCATTT
TTATTTTTACATATCATTTCTCTTTTAAATTAAAGAATAACAGAATGAAACTTGCAAAAGGGATGTCCAC
TCAGGCACCCGCTGGCAGCAATGTTTGGAAGCATTTGCATGCTCTAGGGAGCTAGAGTATCCAGCCCAGG
GTTCCTGTGCTCTTTGCACAAGACTTTCAGGACTTAAGAAAACTAATGAAAACAAACAAACAAACAAACA
AACAAAAAAGAATTGTTAAAACATAGCATATGGAAGAATTGTGGAGTGAGAGGGAAACAAAGCCCAGCAT
CTGCCAATCAGCCTGAAAGCCAAAGCCTGGCTACATACTGCACACTTTTTTGCTTGTGCAGATTTGATTA
AGAGGCGACCTGCCATCATCTCCACCCTCTGCTGGTCTTCTATGATCTAAGATGTAGGTGATGCCGAAAT
GAAGATTGTTCCTTGGACCATTGCCAGGGCTCCCTTCAGCCTGAAATATGGTTCACGTGTGATGCTCAAG
TGCATCTGAGGCTGACAGAGGAAATGCTTGGCATGTAGCCAAAGGGGAAATCCCTGTGTTGAGAGCATAT
GAAGAGATCACCAGGAGATGCCAGGAATCTCTGCCTTTTTTCCCTGCCCAGAGTCCTACCATCTGGGTTT
CACAGCCGTGTATAATTGCATCACCTTCTCTGGGCATTTCTCTCTACAGCCTGGCAGATTTTTATTTTTC
AACCCAGTGTGCACAAAACAGAAAGAATTTCAATTTTTTTCCTTAGCTTTTTACTAGTGCAAGGTATTAT
ACAATGTCATCTCACTGCATGCCCAATAGCTCTTGAAGATCACATAAGCCTTATGTCTTTTTATATGAAG
GCAGTCGGGCTCAGAGGAACTAAACCAACACCAAACAGCTACTGAGTAGTAGGGCCAGAATCCAAGTCCA
TGTCTGCTTGACTCCAAGCCTATGAATGTAGCAACCAGCACCATTTCAGCTTGTACTTGTCTTGACTATA
TGATAATTTGGACCTAGACATGTCCTGACTCCTAGTCCTGTCTCTGTCATGAAAAAGAAATCATAGCAGG
GCCCTCAAGAGGTTAGCAGTACTTCTGTTCTCTCAAAGGTCGTGATTTCTGACCAAGGCCTGTTTATTTT
TAAGGCATTTGCCTATAACATTTAAGCCTTAATTCATATGGGTTAGACTGTGGTCTCTCAAATCTTGTTC
CTGACGGGCGGTGGCACCCTCCAGGATAGATGAAAAGTATTTCTGAAGGGAATTAGTAATCTACACTGAT
TATCTCAACCACATTTATGAGTGAGGTATAATCTTTACAGTCTAAAGGTTTGTGTTGCCCTGGTGTTAAT
CTAGACTGGGTTACAATACAGGATGGTTACTGACTTATCTATTTCATGATAGGTTAATCAATATGGTACA
GTCTTCTGCCAAAGAACACCTTGAATTTTAGGAGTTCTGGACTCTCAAACCTAGCTAGCACATTTGCCCT
CACAATGTGTTCAATGGATCATAACATCAGCTTTCAGCACCTCCAAGACATGTTAAGTCAGACACTCTGT
TCTGGGCTGAGACCCAACAGTCTCCGTGAACCCAGGTTATACACACACCCAAGCTTAAGAAATGCTGCAT
TTTGTTGTGAGGGAGAGAATTATTTTAGAATTTGGAGTAATCTCTGCCTAGGTTTAAAAAAACCTAATCC
ACTACAGGTCCTTGTCAGAACTTGAAAGTTTACCCTGTGTCACCTTTCCCACACCTAAGCTGGTCCTCTA
GCCTGGGGCATCCCAGTGCCTACTGCACAGCAGGGTGATTCGGATGCCCAGTGACACTGTTCTCGTCCAT
ACTGGCAGCATGGCAGTACTTTCCCATTCTGTTCAAAGCTCTTTGGGTATTATGTTAATATTACAATCAT
AACTAAGGAAATATCTGATGACTGTGGACCTGGGAAGCCACGGTCATCTCTAAGTTTATGTTTCTGAACC
TTAAAAAAAAATAAATAAGATGGGTGTGGTGGCTCTCGCCTTTAATCCCAGCACTCAGGAAGCAGAGGCA
GGTGGATCCCTGTGAGTTTGGGGTCAGCCTTATCTACATAGAGTTCCAGGACAACGAAGGCTGAAAGTCT
GTCTTAAGAAGAGGGAGGGAGGAGATGATTGTGGATATCAGGAAGACAGTTCAGCAGGTCACATTACTTC
TGCACAAGCCTGGTGACCTGGGTTTGAACCCTGAACCCAGAGTGAAAGGAGACAGTCAACTCCCAAAAGT
TGTCCTCTGACCTCCACACATGCACACATACAATAGTCATACATCAATATATTTTTAAAAAACAAAATGG
TATCAGGTAGCTGGGCCCTATGATTATTTAAGCTCCAGGTAGCCTCTAGTTCTTTATTTGGAGATGTTTT
TACCCAAATCTAGAAGAGACCTCCTATAATCCTGGTCCAACAGTGTGAAAAGAAAGGGGAAAGTGAGTAT
TTGGAAGGCAGATAAGCTGTTGATCGGGTTAATTGGATAACAGTTGACATTCTCTCTCCCATTTCCCCGC
CTTTTCCTTCTCCTCTGCTCCCCTTCTTCCTCCCTCCTGCCCCTCCTCCACACAGATGTGTCCTCAGAAG
CTAACCATCTCCTGGTTTGCCATCGTTTTGCTGGTGTCTCCACTCATGGCCATGTGGGAGCTGGAGAAAG
ACGGTAAGCAGCCTTTTCCCTGTTTTCTTTGGAGCGCCAGCACCTAAACTTGATCTCCAGGCAGCCTGCT
GGGAAGGGATAGTGAAGTCTGAAGTAGGCAACCGTGCATGGGATTGTCTCAGGAAAAATCCTCAGGGAAG
ATGGAGGGTTAGCGTCTCTTCCTCCACCTATCTCTCTCCCTTGTTTGGTTTTCATGTTGGATGACAGGAA
AGGAGTTTCAGGTGAACTATGGTGGTGTGTGGGATATGATCTTGGCCTTAGATACCAGAAAATGACTCTT
CTAAACAATTGAAATTCCCTAATAGAGAATTTGTAACGTAGCAAGATTGTCTTCTACCCTCTCTTGTCTT
GCATATTACAACGTGCTCTCAGTGGGCACCATGATGGTTGTCCTCTGGCTATTTTGGGATTGGCATTCAC
ACTGACTTGGTTTCAGGTCATGGCTCTGCCAGGTATTGATACTGAACCTTTGAGGCTGGGTAGTCTTGTG
ACCTCAGTTTTCTTTCCTTTTTTTTTCCCCCAGACAGGGTTTCTCTATATAGCCTTGACTTGTCCTGAAA
CTCACTCTGTAGACCAGGCTGGCCTTAAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGAGATT
AAAGGAATGCACCACCACTGCCTGGCTGACCTCAGTTTTCTTAATTGGGTGTTCAGTATAGCACAGCCCA
CATGGACTATTGAATATGTTAATAAATACAGAAAATAACATATGGTAGAGTGAGCAAGCACTATTATTTA
AAACAAAAAGTCTCTGAACTTTTCATTATCCCAATGGCAATAAGATTATTAGTAGACAAATAATGAAGGC
ATAGAAGGGGGTGCTCTCTGTCCTCTGTCCTTGTGCCATGTCACAGTTAGCAAATGTCCATCAACTCAGT
CCTCAGATGGTCGTCACAGGATCTCAAATGTTTCATGGAATACAAGGTAATCAAGTCAGGAATTTCTGCT
TTTATAGCTACACTTTAGTAATAATTAATATTTATTTAACACTCAATTTCGCAACAACCATGTAAAGTAG
GAGCTATCTGTATTCCATTTTACAGATAGTAAAAACTGAGGTTCAAGAGGCTAAGTAAGTTTCCCACAAG
GTCCAGAATAGAACTTGATCCCAGTTTAGTCTCACTTCAGAGCCTGTGTGCTTAAATGCTAGATTTTACA
AGTAAGGCGTGAAGCAACTATTTCCTGTGGCCATAAAGAGGAAATGGGTGTAAATTATAAATGAAAAGAT
TTCCCAGCTGCTGATTAGAAATAATCTTCTGAGAATCCAGGAAAAAAAAAAAAGGTGAGCCTTTTTTTCA
TTCTGAAGAATTACAAGAGTTTGAACGATATTCGCAGGTAAAGGTGCTTGCCACCAAGTCCGATGACCTA
GACTTCATCACTGCGATCAGCATGATGGAAGGAGAGGATCGAGTTCTCTAAATTGCCCTCTGCTCTCAAG
ACATGAGCTGTGACACCTGTGCACACACACACACACACACACACACATGAAAGAATTAAATGCAACTTTA
AAAAGAGTAATCTCCAGCCTGGCCACCCACGCCAACAGAATTTTGGATGCTTTCCTAAGTAAAGCCTTTG
CCACAGCCAGATGGACAACTTAGGCCCTGGCAGAGTTAACCCCTTAGTTACTTCTGACCTGATAAACCAC
AGAAATTTCAGAAAAGCAACAGGAGAAACACAGAGAGAGCTTGTGTTGGAGTCACAATGGTGGCCCGAAG
CCCAGCAGATATCTAAGCTCTGATTCCAACGTGTCCAAGAAGTTCTTTCCCTGCCCTGGGGCTAAGGTCT
TCTAGGGTTTCTGAGTTCAGGTTCTAAAAGGAAAATAATAGACAGCCTGTTTCATGGCCTAGGGGACAAA
GGGAAACTGGGAGCCAAAGGGTCCTGTGCACTGGAATCAGCTCAGGCTAACGATTTCTGAAGAGCTTGGC
CTCAAGTGATTTGTACCTCTTATCTTTGCTCCCCATTCAGCAGTGACAAATCCCACTGATGTAAAAGTAA
GAGCTTAGCATTTCACAAGGCTTTAAGCTCCAATGCCAATCACAAAGCCACCATTCACAGGGCACTTAAG
GAAACTGTCTTAAGCTTGCATACAAATTAGTTTAGATGTTACCTTCTAAAGAAAGCACTAATATTTTGCT
TTCTTTTTTCTTTTTTTAAAAGAATGACATCAGACTAGGGCATGGCAGTTCATGCCTGCAATCCCTGAAC
TTGATAGATCAAGACAAGAAGAAAGCCCAATTTTGTGGCCAGCCGGGGCTATAAAGTAAAATTCTAAGTA
GCTGGCTGTCTCTTCCCACCCCCGAGGCCATGGCTCTCTGAAAGCCCTTTGCTGGCTCTGCTCCATAGCC
GTGCTATTCCAAAACCCAGGCTCTTCACCACTATGCCTTCCATATTCAATCAGGCATCAGATGACTGGGG
CCATCCTGATCTGACTCAGCAACCAAGAACAAACCTCACTGATGGGTTTTCAGGTGATGCTGATGATGGA
GGTTGAAAGACGACCCTGCTTTACGGGTCACTGCATTAAGCTAGTCTTTCGATTAATAATTGATATGTAG
GTAACCTTTGAAGCTTTGTCACATGAGGCTTTGGTGTGACCCACTCCAGAATACCTTATGGAGATATTCT
GGAGGCTCCTTCAGAAACTAGCATCAGATATCCATTGCACAACACAGGAGACAAAAGATCCGAAAGACAC
CATTTGTTACCCTTCACACTTGATGGGACCTCACTTAGCCCTTGGCCATCAGCAAAAACAGTTCCTTCAA
AAACTGAGACAATCATCTACTGCTTTGTAAGAGTGTAAAATAGCTTTTAAAAACAAGTAGTTAATGGCAA
TCTCCGTCCACAAATTTCATGCAGAAGAGACTTAAAAAAAAAAAAACCTAAATCTTTCTGGATAATTTTA
AGAGTTTTTCAGTGCATTTGCCTGGCTCTTTGGCCTGTGCATGACAGTGATGATGATGATGGTGGTGGTG
GTGGTGATGATGATGATTTTAAAAAGCTAGCACAGGGTTATCCTGCCGTAACCTTAGCTGTGCTAAGGTG
CATGCTTTTTTACCATCTATCCTCACAGCTAATCCTATTAGCATAGACAATATTGTTCTTACCATTTTGT
GAGGACTCTGGGGCTACTGGTGGTTCAGGACCTTGCTCAAAATCTCCTTTGCAATTAGAGAAGATAGGAT
TTCTGCATCTTTCTTTCTCCACACCTATGCTTTAAGCCACTATGTTATTCTGCTCAATACTCTGCCCTGT
GTATTCCCTGTGAATTACAACTGGAAGCTTACTCCCCCTCATCTCCATTCCCAACACACACACACACACA
CACACACACACACACACACACACACACACTAGTGGCTTCTGACAAGGAATCTTGTGCTGTTTGCATCTTT
TCTTGTTTCAAGGTCTTCCCTTCCTAATCAGAGACCGATTATTACTGGCCACCTGCCAGTTAAATGACTC
GGTGGCAGCTATAGAACTGTTTCTCTGTTCTGTCCCCATGCCCTGAGATCTCTCAGCAAGCACTTGCCAA
ACTCCTGTGAGCTATGACGTTCCAAGGGCCATGGGTGTGTCAAGACACTGAGTGAAACCCAGTTCCCGGG
GTTTGTGAGGCTCCTGGTCCAGGTCCTCACTGCTGGTGCTCTCCAGTTTATGTTGTAGAGGTGGACTGGA
CTCCCGATGCCCCTGGAGAAACAGTGAACCTCACCTGTGACACGCCTGAAGAAGATGACATCACCTGGAC
CTCAGACCAGAGACATGGAGTCATAGGCTCTGGAAAGACCCTGACCATCACTGTCAAAGAGTTTCTAGAT
GCTGGCCAGTACACCTGCCACAAAGGAGGCGAGACTCTGAGCCACTCACATCTGCTGCTCCACAAGAAGG
AAAATGGAATTTGGTCCACTGAAATTTTAAAAAGTAATTCCATCCCCCTTGACAGTATCTTTTCTTCTAC
CCCTCCTTAAAAAGTAATAATTGCAAGTGTGTGTGTGTGTGTGTGTGTGTGCGCGTGCGCGTGCGCGTGC
ACTGAGGCTCAGAAATGTTTCTCATTTCCTTTGAAACAGGAACTCTCATTGGCCCATAGCTTACCAATAA
GCTTTCTTGGTTAGCCAATAACATCCAGAGACCTTGTCTCAAAAAAAAAAAAAAAAAAATCTCACCCTTG
TACCATGTAATGGCCAGTGATCCATCCACAGTAAAAAAGAAATAAATAAATAAAATAAAAATAAAAAATA
ACAAAAACAAATTTCTTTATTAAAAGAAAACTCCTAAATATTTAATTTTTCTTTTCTTTTCAAATGCAGA
TTTCAAAAACAAGACTTTCCTGAAGTGTGAAGCACCAAATTACTCCGGACGGTTCACGTGCTCATGGCTG
GTGCAAAGAAACATGGACTTGAAGTTCAACATCAAGAGCAGTAGCAGGTAAGGGAGCTGCCATGATTTCT
CAAGGTTTTGTGTGATCTACTTCATAACAAAAAGAAGTACAAACTGCCCATGGATTATCAGTGACTGGTA
ATGGAACAGCAAAGTCAATTCTGTATTTTTTTTTTCAACTTCCCTCTGCTCGCCTAAGGAAATAGCATGG
AGCTTTAAGCCAGCCCTCTGAGACTAGTTGGCTGGGTAGTTTGAATTCACTCATCCGAATTGCCTGGCCG
TGCTTCATTAAGGCTCACAGCACCATGGGGAGGAATTAAAAAGAAGCCACAGCTACAAATAACTGGCCAG
AGCTCGACCTTGTGTCAAAAGCAGAATTGAGTGCTCCAGGGCCTTGCTGTTGGAAGCCCTGGAACAGATT
CCAGAACCCTCTGAGGCCTTTCGAGATTTTCCTAGAGCCTTTTCAACTTCTTCCCCCCCCCACCCCCACC
CCCCAACCGTTATTCTGAAGTTCAGTTTCCCCAATGCACTTGGGCTTTTCCTAAAAACATCATCTCCCAG
GGCTGCAGAGCGGGTGTGTCAGACTGCTCTACAGCTGACTTTAGTCAGCAACTAAATCCCTGAGCCACAT
GGAGGGCAGGGAGTGAGCGCCCTGGCTCTCCCACTATTTAAATGAATAAACGGGGGACAGGGGGTGTGGG
GGGGACGACTCCCTTTTCCATCTTTATCATGAATTTGCTATGACTTAACTTAGCTAAGGAAAATAATGGT
TCAGGGTGACATCAGAGTCCTTGTTGCTTCTATGAGAATTTTCTGATCTGGTAAAGGTGGCCCCAAAGGA
GACCAGTGTACAATGCAGATGCTTACATATAGAAACATGCCAGACCTCTATGTGCTTTAAAAAATTAGAC
AAGCACGATACATTTTCCTGCTAGAGAAATCTTGCACGTGTTCCTGAAGAAACTAGTACATGGATAAGGG
TCAGTGGAACATTTTGCATAATTAGCAAAAGCATGCTCAAATGGAACATCAATGTGCATCTATTGAAATA
AATAACTCAACTTTCGTGTTCTGTTTAGAGAACTCCCCCACAATACTTTCAGAGAGGAAAAAGACGTTAC
AAACTCATGCATATATATTTTTCATTTGTCTTTAAAGTTGTGCTACTCTTTGGATAAACTACATATGTAC
ATATATGTCTGTGTGCATGTCTATCTTAAATATTCATTTGGAAAAACTCTAGAAGTAACAAACTGTGAAC
AATGTCTATATGTGGGACTCAGATTTGAGTAAAAAGGAGAATTGTGGCGACACGCATGTTTTCTTTATAA
TCATCTTTGTTGTTATAATTCTTTATAATTCAGCAGTTTCTCTTGCATTACGATATTTTCAGGTTCATTT
AGGCAGAGATGCAACTTTCAGTCTCTTACAGCTAAGCTTCCACTCTGCAAAGGGCAAGGGGGTTTTTGTT
TGGTTTTGGTTTTGGTTGTTTTATTCCACTGGAGAAATCCAACCAGCTGGACAGACTGGCCTCGGACTGT
AGACACCAGGCACTCTATCTCTGATCTATAATACAGAACTCATTCTGTCTTTGCCATCAGTTCCCCTGAC
TCTCGGGCAGTGACATGTGGAATGGCGTCTCTGTCTGCAGAGAAGGTCACACTGGACCAAAGGGACTATG
AGAAGTATTCAGTGTCCTGCCAGGAGGATGTCACCTGCCCAACTGCCGAGGAGACCCTGCCCATTGAACT
GGCGTTGGAAGCACGGCAGCAGAATAAATATGAGAACTACAGCACCAGCTTCTTCATCAGGGACATCAGT
GAGTTTTGCCTGGGAGCAGGTGCTAGGGAAGCAAAGACGATGTCTGCCACGTGTCCATGATACCCTGGGG
CATAGGATTGGCCTGCATTCTTTTCCATTCTCCTAAAAAGCATGATACAGACACTACCCTCTTCATCTTA
CAGATGAGGAAATTAAAGGGTTAAGCTAACCTTTTTCCAGATCTCAAATCTCACAGACAGCAGACCAGAC
CCAGACCTGCCCCAATGGTGGCACCTAATGGATTATGGGTTAACTACATGCTTAGTGTAAAGCAGAAACC
AAGCACAGGGAGGGTTCCCTGATTGCTGAGCCCAGATACGAGACTCTATTAAGCAGAATGACTCAACCAG
TAGGGACTTCCGGTCTGTGCAGTGCAGAAAGGGTTCTAAAATGTAGGATATGGCCAATAATGGTGACTTC
CTCGAGTGGAAAGATCACTCTCAGCAGATCCCTCACAGGTGCTGCCCTGATGCTCAGAGAAGTGAAAGAG
CTATTCCAGAGACTAGAAGGTGGAGGGCTCTGTCAGCCAAGCTCAGGAGCCTAAGTCCTAGCTCCAAAGC
CTGGCCAGGATAATAAAGGATGCTTGTCTTCTTCTGCAGTCAAACCAGACCCGCCCAAGAACTTGCAGAT
GAAGCCTTTGAAGAACTCACAGGTGGAGGTCAGCTGGGAGTACCCTGACTCCTGGAGCACTCCCCATTCC
TACTTCTCCCTCAAGTTCTTTGTTCGAATCCAGCGCAAGAAAGAAAAGATGAAGGAGACAGAGGAGGGGT
GTAACCAGGTAGGATTTATGCAGCCCATGTTGGGGCCATTGGAAAGATCCTGACAACCAGGTGCTTGCCT
CAGGACCAGAACTGTGTTAAGGGCTCTCCATGTGAATCACACACAACCTATACAAAGTCCCAAAGGCAGC
CAGTATTGCTGTCACCTCTCCACAGAGGCCAGGAAAGCTCAGAGAGGCTATGTTTTGCCCACGTTCTTCC
AGCTAGAGCAGTGGCAGAACCAGTACCCAGCAATGGTTTGATGGAGAAAGAGATGGTTAGCTCAACCACT
TCAATTCCTCCAGTATAGTCGATTGTCAATCCTCAACCAAAGTTGTTCATGATATAGAAACCTGGGATCC
AGGAGCCTATAATGATTGTTTAAACTGAGTAAATATAGTAAATGCTAAAACATTGATGCTGGGACCCACC
AAATAAGGATAATCTCTACTTCCTTAATATGAGCAATCATTTCACCTTACAAGATGTCCACCTTTACTGG
GCTAATATGGTGACTTTCTACCTTTCGGGTCCTTAGCACAGTGATCTCTTGCTAGGTTTGAGGAACACTT
TTATGAGCATTTAAGTCACAGAGCAAAGGATTCTTCCAACCTAGCATTGAGAATGTGATAGCAATTCAGC
TCAGAGATTCAACCACTTCCTAAAAGCAGGAGCAAAAAATGGGATTATTTTGAAACAGATTTTAACTGCT
ACCTAAGTTTCATCCCTGTGTGTATGTATTTAATATACCAATCTTAAATAGGTAATGTCTAGTGCTTTCT
ACTTGCTAAAAGCTTTCTACGCATTTTTACATAAATGTTGTATTTAGCTGCTGCAATTCTTAGAGGCAGG
CATCCTGGGACCTACTCATGAATAAGGAGAGACACAGAGAACCAGAGATATTTTCCTGAAGCACCACAGC
TGTTTAGTAGCATTGCCAGGATTTGAAGTCACTTTTGTAAGCTGACAGGCATGGTAATTCTTGATGTCAA
GACAGCTAAAGAAAGCTGGTCTCAGTCCCAACATCAGCTTCATATGGGAGTGGTTACCCATGGGACCTTG
GGGTAATGCTGCTACTTCAAGAGGCAGAAGGGGGAGCAAGCCATTTCTGTTGGAGTTTACTAAATAGAAA
AGTGAGAGTGGGGTGGTGGATGAATCATGCTGGAATATGAAGACCTGAATTTTAACCCCCAGAACCTCTG
TAAAACTGAGTAGTAATGCCTGAACCTGGGCTCTGGAAAGAGGTGGGACACATACACACACACACACACA
CACACACACACACACACACACACACACACACACCTCTGATTATAGCTTCTACCAGCAGGTGGTGCAAGGG
GAGCGCCCATGCCAGCCTGCCTTATATTGCCTAAAGCCCTTACTTAAATTTTCCTCTCCTTTGCAGAAAG
GTGCGTTCCTCGTAGAGAAGACATCTACCGAAGTCCAATGCAAAGGCGGGAATGTCTGCGTGCAAGCTCA
GGATCGCTATTACAATTCCTCATGCAGCAAGTGGGCATGTGTTCCCTGCAGGTGTGTGAGCAGGACCGTG
AAGCCCAACCACACCTGCACATTTAGTGGTCCTGGTGGAGGACAACAGGAGGCTTGTGAGGGAGGTGGGG
GTGGAATTCCTGGCATAGGGATTCCAGTCAGAAACACATTTGTTCATCAGCAGATGTTAACTTGATGTGT
ATGTTAGGTCAGGCACTATGCTAGGCCAAGGATATTACAATAAAGAAAACAAGCATGGGTCCTACCCTTG
TACAGGGAAAGGCAAGAAGGACCAGAGGAGGAGGAAATCAGGAGTCAGAGTATGGAGAACTTGGAAGTAG
ACAGAAGTTGGAACCATATCTTAGGGGTTCTGGGGAACCACTGAAGTCTTTTTAATAAGGAAATATCCCA
ATCAAAGCCCTTTTAAGAAAAATCTCTCAAGAGAGTCCCTGGCAAGCATGAGACCAGCTGAAGTCCAAGC
CCTGTAAGGAAGGGTTGGGCTGTCTCGCACAGAAATCCTCAAGACCCAGTGTCTGGGGATAATAATGTGT
GGTGTGAAATAGGAGGGTATTGAGGCTATACCTCTGACCAATATGGACTTACCTACCCACATTCCTTTAC
AGGGTCCGATCCTAGGATGCAACGTTGGAAAGGAAAGAAAAGTGGAAGACATTAAGGAAGAAAAATTTAA
ACTCAGGATGGAAGAGTCCCCCAAAAGCTGTCTTCTGCTTGGTTGGCTTTTTCCAGTTTTCCTAAGTTCA
TCATGACACCTTTGCTGATTTCTACATGTAAATGTTAAATGCCCGCAGAGCCAGGGAGCTAATGTATGCA
TAGATATTCTAGCATTCCACTTGGCCTTATGCTGTTGAAATATTTAAGTAATTTATGTATTTATTAATTT
ATTTCTGCATTTCACATTTGTATACCAAGATGTATTGAATATTTCATGTGCTCGTGGCCTGATCCACTGG
GACCAGGCCCTATTATGCAAATTGTGAGCTTGTTATCTTCTTCAACAGCTCTTCAATCAGGGCTGCGTAG
GTACATTAGCTTTTGTGACAACCAATAAGAACATAATATTCTGACACAAGCAGTGTTACATATTTGTGAC
CAGTAAAGACATAGGTGGTATTTGGAGACATGAAGAAGCTGTAAAGTTGACTCTGAAGAGTTTAGCACTA
GTTTCAACACCAAGAAAGACTTTTTAGAAGTGATATTGATAAGAAACCAGGGCCTTCTTTAGAAGGGTAC
CTAAATTTAAAAGAATTTTGAAAGGCTGGGTATCGGTGGTATATGCTTTTAATTCCAGCACTCAGGAGAC
CAAGGCAGGCAGATCTCTGTGAGTTTGAGGACAGCCTGGTGTACAGAGGGAGTTCCAGCACAGCCAGTGC
CACACAGAAATTCTGTCTCAAAAAC

Protein Sequence : Show Sequence

>gi|6680397|ref|NP_032378.1| interleukin-12 subunit beta precursor [Mus musculus]
MCPQKLTISWFAIVLLVSPLMAMWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIG
SGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCS
WLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELAL
EARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEK
MKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS

Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis FTL0552 mutant vaccine , Francisella tularensis guaB mutant vaccine

13. Il4 (interleukin 4)

Gene Name : Il4 (interleukin 4)
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16189
NCBI Protein GI : 10946584
Locus Tag : RP23-188H3.4
Genbank Accession : NM_021283.1
Protein Accession : NM_021283.1
Taxonomy ID : 10090
Gene Strand (Orientation) : ?
Protein Name : IL-4, Interleukin 4

DNA Sequence : Show Sequence

>gi|10946583|ref|NM_021283.1| Mus musculus interleukin 4 (Il4), mRNA
GGATCCCCGGGCAGAGCTGGGGGGGGATTTGTTAGCATCTCTTGATAAACTTAATTGTCTCTCGTCACTG
ACGGCACAGAGCTATTGATGGGTCTCAACCCCCAGCTAGTTGTCATCCTGCTCTTCTTTCTCGAATGTAC
CAGGAGCCATATCCACGGATGCGACAAAAATCACTTGAGAGAGATCATCGGCATTTTGAACGAGGTCACA
GGAGAAGGGACGCCATGCACGGAGATGGATGTGCCAAACGTCCTCACAGCAACGAAGAACACCACAGAGA
GTGAGCTCGTCTGTAGGGCTTCCAAGGTGCTTCGCATATTTTATTTAAAACATGGGAAAACTCCATGCTT
GAAGAAGAACTCTAGTGTTCTCATGGAGCTGCAGAGACTCTTTCGGGCTTTTCGATGCCTGGATTCATCG
ATAAGCTGCACCATGAATGAGTCCAAGTCCACATCACTGAAAGACTTCCTGGAAAGCCTAAAGAGCATCA
TGCAAATGGATTACTCGTAGTACTGAGCCACCATGCTTTAACTTATGAATTTTTAATGGTTTTATTTTAA
TATTTATATATTTATAATTCATAAAATAAAATATTTGTATAATGT

Protein Sequence : Show Sequence

>gi|10946584|ref|NP_067258.1| interleukin 4 [Mus musculus]
MGLNPQLVVILLFFLECTRSHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCR
ASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS

Molecule Role : Vaximmutor
Molecule Role Annotation : IL-4 plays an important role in Th2 immune response.
Related Vaccine(s): LVS

14. Nos2

Gene Name : Nos2
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 18126
NCBI Protein GI : 6754872
Locus Tag : RP23-341J22.2
Genbank Accession : AF427516
Protein Accession : NP_035057
Taxonomy ID : 10090
Gene Starting Position : 78920858
Gene Ending Position : 78960123
Gene Strand (Orientation) : +
Protein Name : nitric oxide synthase 2, inducible
Protein pI : 7.76
Protein Weight : 123528.57
Protein Length : 1144
Protein Note : Also known as iNOS; Nos-2; Nos2a; i-NOS; NOS-II

DNA Sequence : Show Sequence

>gi|372099099:78920858-78960123 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
TGGCCCCACGGGACACAGTGTCACTGGTTTGAAACTTCTCAGCCACCTTGGTGAAGGGACTGAGCTGTTA
GAGACACTTCTGAGGCTCCTCACGCTTGGGTCTTGTTCACTCCACGGAGTAGCCTAGTCAACTGCAAGGT
GAGTCTTGTGTTGGGGACAGCTGCTCTTAGGAGTGTCTTCCTGCTTGGGAAAGCCCAGAAAACCAGGCTT
TCCCTTCTCCCCCCACCCCACCCCTCTTTTTGTGGCTGGACTGAAGCTGATATGGAAGGTGTAAGAAGCA
CACCCACAGAAGGGTCTGCCAGTTGAACAGGGCGTGTGGTCAGATTTCTTCTCTGCAAATGTTTACTGTG
GTGATTACACTACAAAGGATAAACTTTCTAGAAAGCTCCGAATAGGCTGGGGGGGGTGGTGTTAATACAT
ATAGCTTACTTTACTCATCTTTATGAGATGTGCCCTCTGCCCCACCCAGATCTGATAAGATGGTTATTAT
TAATCCCATTTTAGGAGTGAAGCAATGAGTTTCAGTTAACTAACTTGATTTAGATGAGTAACTAGAAAAA
CTCAGGGTAGGGTCCAGTTCATCGGGACTCTGAGTTTCACTGTTCCCCTTAGGTGGGTTCTTGTACCCCC
CCCCCCCGTATCTTCCAAGTATATCTTTCCTCAGGGAGGGCACTCGCCAAGGAGAAGCCACATACTCTCA
GGGCAGTTCCACTTTCTATTTCTTGTCTTTTTCGTAAAGGATATAATCTCAACTCTGGAGAGTTTAATGC
TCTTTTATACAAATCATTGACTTCTTCTCAAGTAACAAAGAAGAAGAAGGAACATGTGACCTAGTGTCCT
TGGCTCAGGATGCGTGATGACTCCCAAATCAAAGGAAAGGTGGCCAGGACTTGAGTGCTAGCCCCAAGTG
CAGGTCCAGAGGGAAGGGTACTAATGACAAGTGCTTGCCCCTCTCTCTGGTCAGCAGGTGGCAGGAAGGG
TCAGGCAGATGCACACACTCCTGTATTAGTGACCCCAGCTCCTGCCCACCTTGCTGGAGGCCATGGAGTA
ACTCCTGCCAAGAGAAACTGAATTGTCCTAAGGAAGCTTGTGGATGCTTGCTGTCCTTGGTAGGTGGCTA
TGTGGCATCCCAAGTTATCCCAAGTTTGGTTTGTTATGTGTCTTTGTAGAACTGGAGTCTGTCCCACAAG
ATTTCAACTCCAAATAAAACTGCCGAGTGCAAGCATGGAGAGAGAGCACTTCTGTGCTAGCCTTAGCTGG
GCTCTGTCTCCATTCTCTGGGACACTAGCGCCCGCCCGTGAGAAGGCTCTGTTCTGTTAGGCTCTTCTTC
AACCTCACCTCACAGCCTCCCTCCCCTTCCTCAGAGAACGGAGAACGTTGGATTTGGAGCAGAAGTGCAA
AGTCTCAGACATGGCTTGCCCCTGGAAGTTTCTCTTCAAAGTCAAATCCTACCAAAGTGACCTGAAAGAG
GAAAAGGACATTAACAACAACGTGAAGAAAACCCCTTGTGCTGTTCTCAGGTAAGCAACCTCTCTCCCAC
TTCCAGCTTTCTCCTCAGGGCTTCCAGGCCCCTGCTGCCTCTCTCTGTTCTGGGCCACCTAAGAGAGTCT
GGTCTCCAAGGCCCTGCTTGTAATACTCTACTGGAGTCTGATGCACCTTCCTTGGTGTGGACAAGGAAGG
CCCAGGATCCCCATGCTGCTCCTGAGTGAGTCTGTACTCTCTGGAGGAAGAAGGAAGGGGGAAGGGGAAT
ATGAGAGAACGGAGACTGGAGAGCCTAGTGGTCAGCTCAGTAGTGACGTCCTGATTGGCCAGGGCATGTG
CCTCACCAGTTAGATATTTTGACTATTAGGCAACTGAAGGGTGTCAAGATGGCCCTGTACTATCCTCAGT
GACTGTGTGACTTTGAGTAAGTGCACCTGTCTGAGTCTCAGGTCTGCAGTGTGATTTACCTGATAGTCAC
AGAAGGGCTTGTCACCATTTAAGAGGCTTCTTTGTCACAGGACTACACCGTGCACACACCTACACTCTTT
GAACGTAACCTTCCTGGGGCCCTTAGTTATGATCTCATTTTGCAAGTGAGGCTCTGGAGGGCAAAGTCTG
GCTTACATGACTGCTAATGTCAGAGACGAAATCTGAGTTTGGCCTGAGGCATCTTAGAATTGAACTAGTC
CTCCAACAATTTATCCGGATGGACTAGGAGAATTCTAGAGAATGCTAGGAAACTACAGCCATAGCCAGGG
ACTTGAGGCTAACTTACGTAGAAAGATGATGAGCTGGCTTTCTTTGCAATGTGACTGCACAGAATCACGT
CCAAGTCTCAGTCTGTACCTGGCTCAACATCTGCAGGACTACAGTGGACAGCGCCCCCTGGAGGCAGTAA
GCAGGAAATCTCTAAATTTCCCTGTCCTGGTGTTGCCAGGTTTAGAAAGCAAGTTTAGGCAGAAGGGAGT
AGCTTTGCCACTTCATAATCCTGTGGCATTTGTGACGTCCCTTAGCCTCCAAACCTCTGCCTCCTCACTT
GCAGGAAAATAGTCATTATGTTAGGAGTAGAGGAGACAGATGGCTCAGGAGTGACAGGCAGAGTGAGTGG
GAAGGGACTGGACCACAGCTGTCAGCAATCCTGGGGCTCAGGTGCCCTCTAGCACTTCAGTCTCAAAATA
CCGTGACTTCATCCCGTTTTTTTCTCTTTACCCGGTCCCTACAACCCCTACCTTCTGTCGCCTCAGAATC
CAGGTGCCTCCACACCTCTTTCCCTCCAACTTGACTTCATAGAAGTCATGCCCCTGCTCCAAAATCTTGG
GTAGCTGCTGGTTACCTGTCATCACACTGAGACAAGAAGGAGGGCACATCCTCTGACAATCCAGAAGGGG
ACTGGGTTGTCCTCCCACTTTAGGGAAGAGGAGACAAAAGTCCACCGTGCTTGAGTCATCTGAAGATGGC
AGTGGTGAAGCAGGCATTCAAACTGGACAGTCTGGCTTATGGCCACCATCCTGGTAGCTACACTGTAGGT
CTCCAATCGAGATGTATCCCAAGGACAAGGACACTTGAGGCTTTCTCACCTGGCACTATACTGCCAGTTT
ACATGTCAACAGAGCCCTTCTGCTTCATCAGTGGATTGATGCAACCATTTATTAACTGCATATATGGCCA
CACATATTTATAGTGTCCTTAACACTATGTCAAAAACCAGGCAGGAACTGCTTTTATCGTGTCTAAAGAG
GTCAGTTTCTAGAAAGTGGAAGAGCTGAGATTTGAGCCTAACTCCTGTCTCTGAGGACTTAGTAAACTCT
GGGCTGAGAAAGAGACAGATCCAGTCGCTACAGTCAGAGAGGAAACGGGTGGGGTGGGGGAGGGGGTTGC
GCATGGAGAGCAACAGTTTCCTCCCTAGTTGGAGGCAGTCTTGGCAAGGCAAGATGTTAGAGAGGTTTAT
GCGTCTCTGAGCCTCCTCCTAAGGGCACCGCCTGGCTGTGGGGTCAGGAGGGCCTAATGAGCTGCAGGTG
GCTTTCTTAGAGGAGGGGGCCCAGCCGAGATGGGACTGCCCAGGAAAGGTGGCAAGAGGATGTTCCTGGA
AGAGGAAGCCGCGTACAAAGGGGATTTTAGTCTGAAAGCAGTGGGAGCCCGTTATTTATTTTTATTTTAA
GACATAATTTTCAAATCTATGCAGACGAAGAGCCTATTCCTGGTGGCTTTCAACTTTGTGTAAACAAGAG
ATTCAGAAGTGTGCAGGGTGTGTCCTTGCATGTGTTTTAGAGACAAAGGATAAATGTGGGACCGTCTAGG
TGACCCACAGGGAGTTTAGAGCAAAGGGTTTCAGGATGCTTAAAAGTACAAAACAAAACAAACAAAAACA
CCCAAAACACTCCTCGCACTGTAATCGGGCTTGGGGATTCATTTGCTGAAATATTAGCCAGAAGGGTCCC
TGAGGGCTGTAGCCCTATTTCACCTGCAACAGGGAGAAAGCGCAAAACATTTCCTGGGAGCGCTCTAGTG
AAGCAAAGGTAAGAGATGACCCTAGGGCTGTTGTATGGCCTTGAGCAGGATCCTCATTTCTCTGGTCCCC
CAGCTTCTGCCGCCATAAAGCAGGGGTAGTAAAGAATTGCCTTCTTAGTTGGATTTCGTTAGGGTGTGAG
GACATAACCCGGTGCAGCGGGGAGTCTGGGTCGCGATCCTTACTGTCCCTTCGAGAGGAATACACAAACG
AGGCACTCGCGCGCTCCTGCTTGTCCTGGGCCACTAAGAGCTGAGTCCTGCCCGCAGCAGGAGGGCGGGC
GCTACGGGGGCGGGCGCTGAGGGGTTTCGCGGCGATAAAGGGACAGCGCCAAAGAGGAGCGCTGGCCCAG
CTATGCGCGAGGGGCTGAGCAGCGCTTGCGACGCCGCCCGGCCGCGGCTCTGTCGCTTCCTGCGCAGGAT
CTTCGGCCCGAAGGTCGCCAGTCGTGTGGGCCCAGCCAGGTACAGAGCGCGGGACCTGGGGAGGGGCCCG
GGTGGCCCCGCTGGGACGCGAGAGAGGCGGGTCAGAGCGCCCACGCCGGCGGCCCTGTAAGGGTTTCTGC
ACCAAGGAACTTTTGCGCTGCACTGCTTTGGACAGGTTCTAGCTATAACGATTACTTGGCGTCAAACCTG
GAAATCTTCCTGCCTCGGCTTCTCAAATGCTGAGATTACAGGCGTGTGCCACCGCTTAAAGATGTTTTTT
TGTTGGGGGGGGGGGGGGAAGCAGTACTGGGAACTGAACTGCATTTAAGCAAGTGCTCTTCCACTGAGCC
ACTCTCCCCCTCCCGGCTTGCTGCTCTCCCTCCTTAATGCGCTCTGAACAAACAAGGTTAAGAAGCCAAA
GATGATCTTTTACGATAAGACGTTCTCCCTTTCACAAGAGAGGTGGGCTGGCTGGTAGCAGTTCGATCCC
AGGACTCAAAACCCTAAGTAAGCCCCTGCTGTGGGGTCTGCACTCACCCCTCAGTACCTAGTTCTGGGAC
ACCGTGACAGCAGATGGTTCTATGGACCTGAGCAAGCCACCACAGTTCCCTTTGTCCGAAAGTCCATAAA
GGTCGTTCTCCCTGCAATGGGCCAGTCAGGTCTCAGCAAGGCTGTGGGTTTAGAACTGCAGGAATTTGGA
GCTAAGGGTCCCGAGCCTTAAAGTGTTCACCTGATATCTGGTACCTAGATTCCCTTTAACAGGTGGCAGA
AGAAATCTGCTAGCAGGACATAATAGCTGGGGCAAAGCTTTATTTATCGTTGTGGGGTTTTCCCCTTAAT
CTCTATTATAGCAGTACTTGAAAAATTATAAAAGTTCCAATTATAATAAAAACGTCAAAATACTTAAAAG
TTAAAAAGAAAGAGATTTAAGTCACCCGTGGCTTATCACATCGTGCTAATCACTGTGAATCTTTGCTGGC
ATTTCCCCCAGTTGCTTTCAGGCCTGCTGGAGCCTGCATTCATTCACACACAAGCACACATTGCATGCGT
GCACAAAGACTGTCATCACAGCGTCACAGATATCCAGGCTGAGGCATTAGGCACAACCTCAGGCTGTGGA
CCCTGAGGCCTGCCTAACTGTGGCTCATGTCAGCAGTGAGACTGCAGACAGCATTTAAAACATTTTGCCG
TCTGGGGCAGGTTTACATCCTCACCAGAAGGAGGATCCCCAGAAAGCAAATCTTGGCTTCCGGATTTTTT
GGGTTTTTTGTTTTTGTTTTTGTTTTTGTTTTTTTTCCTCCTTCTGGTGAGGTATACCAGTCCAGATCAG
AGGAATATGTTAAAGAAAATATTATTCAGATGGGTGTGGTGGCACATGCCTGTAACCCCAACATTCAGAA
GGTAACATCAAGAGTTCAAGGACATTGTCAGGCAAATGGTGAGTTCAAAGGCAGTGGAAGGTCATGAAAC
ACTGTCACAAGCAAGAAGGAGAGAATTGTTCAATGAGTTTCTTGGTTTGCTTTGGTGGTGGCGGTGGGAA
GTTATTGTTTTGCTTTGTTGTTTGAGATAGCCCCTCACCTGAGATAGCCCAGGCTAAGCAAGTCTCCTGC
CTCACCTTCTGAATGCTGGGGTTTCAGACACCAGTCAGTGTATGTATCCCTCTTTAGTGATACCGGCTAA
CATACCGCAAAGAGGACTTTCCTGAGGACTGTCCCTCAGGTGAGTCCAGGACCCACTGCAGTCCGTAGCA
GAGGGAGAGATAGGGCTCCACCCAAACCCAGCAGGGGCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGG
GCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGGGCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGGGC
AAGGGCAAGTTACAGCCAAAGGTAAAGAAGAGCCTCACTGAACTGACCTAACAATCAAACCAGGGATCGA
GTTATTACCAAAACACAAAGCAGGGCCATAGCTAAGCTGGAGTTTGGGGGGTTTGTTTTGTTTTGTTTTA
GATTTATTTATGTGTATGGATGTTTTGTCTGACTGTATGGGTGTATAGCCATGGCTGCCCTGGAACTCTG
TAGACCAGGCTAGCCTCGAACATAGAGCTCAGCCTGCCTCTGCCTCTTAAGTGCTGAGATTAAAGGGACC
CACCACCATAGCTGGCTATATATATATATATATATATATACACACACACACACACATATATACATATATA
TATATATATGCACACACACATATATATACACACACACACATATATATACACATATATATGTATATATGTG
TGTGTTTCTTTTTATATTATATATATATAATTTTTATAAAATTAAATTTATTTTAATTTAATTTTTATCT
TATTTTCAAAACAGGTAGCATATAGCCCAGGCTAGCTTTGAATTCCCTACTTAGCTGAGGATGGATGACC
TTGAACTCCTGATCCCCCTGCCTCAACTTTCCAGGTGTTAGAATCATAAGTGTGTGCCTTCTGTACCTGC
CTTTGTTCCTTTCACTCTTTACAAGCACGGGGCTTGAACCTGGAATCTCTGCACTCAAGAAGCCCTGGCA
AGCGGATGGAGGCCAGTGCGGTGCACAGAGTAAACAAACGAAATCAAGGGTTTGGGGTGCTTTGTAGGTT
CTCTTGGAGCCCAAACTGGCTTTGAAGTTTCTTTGTAGTTGAGGTTGGCCTCAAACTCCTGACCTTTCTG
TCACAACCTGCCAGATGCTAGGATTCCAATTTGTAAAATGCCCAGCTTGAACTAAGAGATGAGCTGGGCC
TGGTGATGCATGCTTTTAATCCCAGCACTCAGGAGCCACAGGCAGGTGGATCTCTGTGAGTTCAGGGCCA
GTCTGGTCTACCTAGAAAGTCCCACATCTTAAACATACAATACTCACCAAAATGAAAACCGAAAATACAT
AAGATAAAGTTTGCTAAGCCAAGTAGGTGGTGAGTGATGGACCCTGCTGGCCTGGGAGGGCTTTTGCACT
TGGCCCTCTGAGGCAGGCATCTCCCCTGAACTTGTGTGTGTGCCTCTGTCTGAGTAGTTTTGGCTGGCTC
CCCTCATATCATTTTGGCTTTGAGTGGTCAAAGCAAAGATGAAAAGGGAAGTGGGGACAAGATTCTCTAA
GACTGGCTCCCTCATCCTTCCTGATTGGAAGCCGGGAACAGGTCATTCCCACCTCTAGCCCTTGGTACCC
CGTCTGCCAGATCGGGACTCACAGAGGCCACTGGAGATCCCCTCCGTGGCCAGACTCCATCTCACAATGA
CTGTTTCCTTTCGCAGCCCAACAATACAAGATGACCCTAAGAGTCACCAAAATGGCTCCCCGCAGCTCCT
CACTGGGACAGCACAGGTCAGTGGTTTGGTCATACAGTATGGGAAGCCCTTCATTCTTTTCCTTGGTAGA
TTCAGGCTAAACAGCCCTTTGGAAGCTGGGCAGGAAGGCACAGGAAGCCAAGGGCTGGGGAATAAGTGGA
GCACCCCAGACTTAGGGTACAGAGGGAGCCAAGGAGGTTGCAGACAGTAGGACAGTCTTGATGCTCATCA
ATAGAGGTCCCCAGGGTGCGCTGGTACCTAAGTAACTTGCCTGGTGGTTGACAGAATGGGTCGGGGTAGT
GCCATTAGGGCTCTGTTTCTGTCAAGAGATAGCATGACCAAGGTAATTCTTTTAAAGGACTACATTTAAG
TGGGGCTGGCTTACAGGTTCAGAGGTTCAGTCCATTATCATCAAGGTGGGAACATGGCAGCATCCAGGCA
GGCATGGTGCAGGAAGAGCTGAGAGTTCTACATCTTGTTCTGAAAGCAAACTGGAGAAAACTGTCTCTTC
CAGGCAGCTAGGAGAAGGGTCTCAAAGCCCACACCCAAAGTGACACACTTCCTCCAACAAGGCCACACCT
ATAAGCAGTATCATTCCCTATGGGCCAAGCATATTCAAACCACCACAGGTAGTTTAGGAGCATTCTCTAA
ATCAGGGAAGGACGGTTTTCCAGAATGATTTCAGATTCAGCTTCTGAGTCCCTAGTCTCAGCCCCTGGCT
TCTTCCATCTTCCTGAAGCCCCTTACATCTTAAGGACTGTAGAGGGGTGGCAGAGAGATGGAGCCGGGAA
AGGTGTCCCTGGGGAAGGGAGACAGTGGAGTATCTGTTGACTTCCCAGCTCTGCCCTTGGCAGTCCCAGC
CACAGCTTCGGCCCTCCCATCAGACTTGAATTCCCCTTATGAGCCAGCACTGGCCAGGCCTTGGTAAGGA
CAGAAGTCTGGCCTTGGTCCAGCCACCTGCAGTGCTCAGCTGGCACTAGCCCAACTTCTGGGAAGGGTGG
AGGACCGAGGACTGGCCCCTTGCCTTGTGTGCTCACGCAGAGCTCCGTCCCTCATCCAAGCAGAGTACAG
AGGCTCAGGGGACACTTGTATGGTTGTTGAGGTTATAGACATTTAGAAAACTCTTGATGTTTACCCATTT
TAGAATGTTCCAGAATCCCTGGACAAGCTGCATGTGACATCGACCCGTCCACAGTATGTGAGGATCAAAA
ACTGGGGCAGTGGAGAGATTTTGCATGACACTCTTCACCACAAGGCCACATCGGTAAGAAGTCTGGGGGC
TGGGGAACACCACCTTTGTCCCAACTAGGCCATCATAAGGGCCCTTTGTGGGTCTATCACATATTTCCTG
GTCTTATTAAACAGCCATCTTCTCAGACAAAAGATGTTGGGAAGTCTGTCTTTATAAATTATAGCAAGAA
GAAGGAGAGGTTAACTACTGGGTTAGACAGCAGATAACCTAAGATGTCAGACAAAAAGCTACAGAAACCA
GTAGAGTATCTGAGAATTGTCTATTAGAATTGCCCAAAGCTGGACTGAACTGGCAGAGGAAGCTTCCTGA
CTCTTTAAGGACAGCAAGGTAAGCCAGGCAGTGGTGGCAAATGCCTTTAACCAGCACTTGGGAGGCAGAG
GCAGGCAGATCTCTGAGTTCGAGGCCAGCCTGGTTTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACA
GAGAAACCCTGTCTCAGGAAAAAAAAAAAAAAAAAAGACAGTGAGGTAACAGTGGTTTAAGCAGCCAGTG
AATGAAACTAATCACCCTTTCAGGTTCCTTTCTACCTTTAAGTACCCTACTCTGACTTTGGAATTCATGC
TATGAGGCTGAGCAGTGCACCAGTGCCCACCTTTAATTCCTTTGATGGAGACAAGCAGATGTCCATGAAT
CCTAGACCAGCCTGGGCTACATAGTGAGTTCAGGGACAAGCCAGAGCTACACTGCGAGACCTTGTCTCAG
AACAACAATAATAACAACAAAACATAAAATAAGCCAGGTGTGGTATACACCTTTAATTTCAGGAGGCAGC
AGAAGAGGCAAGTGGAACTCTGTGAGTTCGAGGCCAGTCTGGTCTACGAAGGAGTCCAGGACAGCCAGGG
TTCTGAGAAACCTTGTCTCAAAACACCAAAACCAAAAAATAAAAAAATGAAAAGCCCATAAAACAAAACA
AAGGGGGGAGAGAGAGAGGGAGGGAGGGAGGGAGAGAGAGAGAGAGAGAGGGAGAGAGAGAGAGAGAGAG
AGAGAGAGAGAGAGAGAGAGAGAATAATTCATACTGTGGTTATAAAGTGGGAAAGAATCTTTGAAGGCAG
GATGATTTGCTGAACCCCCAGAAACTGTGTCTATAATGCCAGCTTGGAAGATAGAGGCAGGAGGATTGGA
AGCTCACAGTGGCCTGCTATGTAGTGAGTTTGAGACCAGCCTGAGTCACAGATGATCTTGTCTCAAAACC
CAACAGCAAACCTCAGCAACAAAGCAAAACAGAGAGACGCCACAGTTGACATGTGGACATCCATCTTACT
GGCATTTCTTTCCAAGCCTCCCCTGACTTTGGAATAACCATTCTCTCACTCAGCTTCTCTGGGCTGACAC
TGGAGCCCTGTTCCCTGAGGAGCTAGCCGGGTCTCTTTAGAAAGGAAAGCAGACCCCCTAGGGAAAGATA
TCCCCTAGGAGGAAGCAGTAGAGTTTTCTCACAGGCTGTCACTGTATCTTTAGCTTACTGGAGAACAGAG
AGAAGCCAGCTCACACCCACCTAGTCTCAGGAAAGGAGAGGGGAGCTAGGCTTGGAGAAAGCTTGGTTGA
CTCCACGGAGAAGGAGCCTGCATGGGGTCATTGTGAGCATACCGGCCAGAGAATGGCCAGGCTTATGACT
GGTTTGGAGCATGGGGGGTCTCGGTGGGATGAAGTGGGGAATAGGGGAATAGGGGCCCTACTCAGCCCAT
TTCCTTTTCCAGGATTTCACTTGCAAGTCCAAGTCTTGCTTGGGGTCCATCATGAACCCCAAGAGTTTGA
CCAGAGGACCCAGAGACAAGCCTACCCCTCTGGAGGAGCTCCTGCCTCATGCCATTGAGTTCATCAACCA
GTATTATGGCTCCTTTAAAGAGTAAGTCTGGCTTTCGTCAGGGTGACATCCCCTCTGCCCTACTCCCTGG
GGCTGACCTGTTTCCTACTGTTCCACTCTGATCGGTGAACTCTGCAGTCTCCAGACTTGATGGGGCTAAA
GTAGGCTCAGGCACTGCAAAAATGTCTGAAAGTTTGGAGGAGAGTGTAACCGGTTTTGGATTCCGGCGGG
CCACCAGGTCACTGCTGAGTCCTGGAGAAATCCATTGGGTCTCAACCTCCTCATCTGTAAAGTGGGCATC
TTATAGCATGACTCACTAGACAGCCATGAGATGTGTACAAATTTAGAACAGTGTTCTAGCCTAGGGACTA
TAATACAGTGACTTGGAATGAATCCTTTTATCCTGAAATAGAAGGACATTTAGAGACTGTCTGGTCAGGC
CTCTCCTCCTACAGATGTGAGCAAGATTCCCAGAGACAGCACTGGCATCAGCCTAGTCTAGCTAGAGTCC
GGGAGACCTGACTTGCTGGGGTGCTACATCTCTGGAGTGCTCTCATTTTATGCCATAATGCTTCCTTGAG
TCCAGTGTCCAGACCCCTTTATTCTTTGGGGTTTTGTTGTTGTTGTTTTGTTTTGTTTTGTTTTGTTTTT
TGTTTTATTTATTTATTTGGTTGGTTGGTTGCAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGC
TGGCCTCGAACTCAGAAATCCGCCTGCCTTTGTCTCCCAAGTGCTGGGATTAAAGGTGTGAGCCACCACT
GCCCGGAGAGACCCTTTATTCTTAAGCCCACTAGTCAGTCACTCCAAGCACCAAGTATTGTCCAGCATGA
AAGGCAATTGTTTTTCATTGCCAGTTGCCAATGTTGTTGTCAATGCAGTTGCAATCATGGCATGGCTGTG
TGGCAACTCTTGGTAAGAGTTGGGATCCAAGGAGCCATCTTCTGAGGTTCAAGCCTTGGTTTTCTTGCCT
CAAAATATCTATTATAAAATACCCACCTTGTAGGAGTTTATATGAAGATTAAATGAATTAGAGGCTTCAG
AACAGTGCTGGCCTGTAATGAGTGCAACCGAGATCTTGGTTACCTCGTACGTACTCAAGCTTTTAAACAT
CTGTTTACTTGGTGGAGAATGGGGTACCTGAGCCACAGCAAGTTTGTGAAGGTCAGACAAGCTGTGGGGG
TTGGGCATCTCCATCCACAGTGCGGACTCCTGGGATTGACTGAGGTCATCAGACGTGGTATCAAATGTCT
TTACCTGCTCAGCCCCTCATTGGCCCCTTTACCCTCTGGCCCCTCATTGTTTTGGAGACAGCATCTCATG
AAATAATGAAGGCTGGCTTCTGCCCACAATTGTTCTTGAGAGTGCTGGGGTGACAGGTACAGGGGATGCT
GTCTAATTATAGAACATTGTTGACTGACTGATTTACCTGTCCAGTTGTAAGAATGACTGTCAGAGACAGC
GAATGTCTACTCTGGCAGCTGGGGACGGTGTGGCAGTGTCATTTTGTGGCACAGCCTCACAGAGCAGTTC
CACCTGGGGCGTGAGACACAGAAGGGTATCTGGTACCCAGAGCAGAAGTTTCCATTCTTGGGGAATCCCT
GTTCCACCTCTGTACAGCCCTGGAGTGCCAGAGCCGGGAGGGCGCTTGACAAGAGGTCTCTATCGTCTAG
AGTAGTGCTTCCCAGCCTTACCTGGGAGCTGGCAGAACAGTGATGACTGGTCCCCCCTGAGCCAAGGTCA
ACCATCTTGGGAAAGGCTGTCTGTAGATTTGTGAAGGGTGGAGGCCTGTGGGGGCACTGTCTTTCCTGTA
AGCATGTATCCGGGGCTTTGTGGCATTGAACTTCAGTTTCCCAGATCTTCCCACTCTGTTGTTAACAAAT
GGCTGTTTGGGGGGTCTGAGATTCTAGATAGGCTTCATCTGGCCATTTTTTTTAATTTGGCACCAAGCAC
ATGAAAAACTCTGAGTTGCTGGGTTAACTCTGAGAGCCAGGTGTGGGCAACACAGCTCTCTATAGATAAA
TAGGAAGATCTTAGGAGGACTCCAAGTTCAAGGATTGCGTGGGTGACAGGGTGAATACAAGCCAGCCTGA
GCAACATAGAGGGAACATGTCTCAAAATAAACGGTTCAAAAAACAAGTGCTGGGGCCACAGCTGAGCATG
TAGCTCAGTGGTAGGATAAGCCTGGTTTGGCGCCCTGCTTTTCTTTTGAGGCAGGTACTCACTATGTTGC
CCAGGCTGGCCTGAGTTTACCTAATTTACACAAACTCATTTGGAAATTACAAGTGGGTGTGGAGAGACGA
AGCCTGCCTATGGTTTGCTCCACGTGCCGAGTCCAGAAGGCCAGAGAAACAACTGTCTCCCTCCTGACAC
TCCTGTGAAAGACAGAGGCTCCTGTTTCTTCTTGCTACCATAGTTACTGCGCTTTTCATTACAACAAGAG
GATGGCTTTGCTCAGGACCTGTCCACTCCTTAGAATACAACGGGAGAGGCCAAAACTCTTCCAAAGAAAT
CAGGCCCACTCCTGGGTAAATGACACCAAGTTTTCCTCTTTCTCCTTGAAGCGGTGTTGCCAGACCACAG
ACACTGCTGTTTTCGTGTTTGGGTAGAGTGGGGTGTATTTCTAGAATCTTCCTCTGACGACTAAGGACAC
TCACCTCACCCAGAAGTTCCCCCTCACAGGGATCACTAGAGACAAACATCGCTGAATGTTTTGTCTTCAA
GTCTTTCTGTAGTCAAAAAGGAGTTGCCAAGACCCTCCGCCTGCCCCGAAGGCTGTTCATTCCAAGTCCT
CCTGCTGACCTGAATCCCAAGTTCATTCTTTAAATAATTTTGTTTGTTGCCATTTCGAAAGCCTCTGGTG
GTGCTGGTTGGAAATCTCAAGGTTGCTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
TTCAAAGTCTTTGCGACCAGGCTCCTCTCCCTTGGAATTATTTTGAAGTATGCTTAAATCAGTGAGTGTA
GCTCTGTTGGGTGGGCTCTAGGGTCCTTCAAGTTGAGTCCTGACCTCAGGTCTTGTCCGGGCCTGCCTTG
AGTTTTGAGGAGCCCTCGAGGCTTGCTTCTCCCCTTGGGTGGAAGGTGGGAGGTCCTATCCACGACAGAC
AGTTTGGAGCCTGGGGCTGGCAGTGAGACCACCTGGAACTATGTCCTCTCTGTTACTAGGCTTGTGTTCT
TGAGCCTGGGACTGTATACTCTAGAGTCTTAAGACTGCTGAGCAAACACAGCAGTGCCTCCTAAGTGACT
TGTGGTGGCATGTAGCTGCACTCTCAGGTATTTGAGAGGGGGAGGCCAGAGGGTTGGAGGTTCTAGGCTA
GACAAGACCTTGTTATGACTATAATAATCAGCAGGGTCCAGCGTAGGAACATAAGTGTGGTTTTTCTCCC
AACAGGACTCAAGATGGAGATGGCAGAGTATTAAAAATCGGCCCCTAGTAAGCGAAGAGTGGGTCACAAA
GCCAGCCCTACCGAGGACAATGTCCCAAGGGGCACAATTTGCCCTTCTAGTGTCCAAAATCAGTTCTTGC
TTTCTGGGGATTGAATTAATCTCCATGTAAGGTCTTGCTATTGTTGGTCTTCCTGACAATTTCCCGTCCC
ACAGGGCAAAAATAGAGGAACATCTGGCCAGGCTGGAAGCTGTAACAAAGGAAATAGAAACAACAGGAAC
CTACCAGCTCACTCTGGATGAGCTCATCTTTGCCACCAAGATGGCCTGGAGGAATGCCCCTCGCTGCATC
GGCAGGATCCAGTGGTCCAACCTGCAGGTGGGTCTTACCGGCAGAGCAGAAGGAGGAGCAGGGGTGGGCA
GGCCCTGAGACAGAGCAAGTGGCCAGGGCTCTGCAGACTACAGCTAAACCCTGAGGTGAGGCATCCCAGA
CCCCCTTCTAGGAGCCTAGAATCTTATTTTAGCACCAGGGTCTCTAGATGACCCACCCTATATAAAATGT
TGGGGACCACATGCCATCATGAGACAACCAGCAACCTTGGATGTCTTATTCCTGAGGCCTTGGTGCTACA
AATGTTTGCCTCCAGTGTTTGCGATCCTGGCCCTAGAGAGCATTTTATTTATCGTGGCCTCCTAGGCCAT
CGTCTCCTTGGAGGGTCATTTGGGCAGGGGTGGGGGGTTGGGTCCTATTGAGTGCACTTGGACTGAGAAA
ATCCAGGAGTCTCAATGGTGGTGCACTCCCATGGCCAAGATCCAGGCTAGGGCTGTGGGGATCCTGCCTT
TTGGTTCTTTTCTTCTTTTAAGATTTATTTATTTATTTTATGTATATGAGTACACCATTGCTCTCTTCAG
ACACACCAGAAGAGGGCATCAGATCTCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGA
ACTCAGGACCTCTGGAAGAGCAGTCAGTACTATTAACCGCTGAATCATCTCTCCAGCCCCTTTTGGTTCT
TTAAAGCCAGACACTTTTGGTAATTTGGTTTTACTGGGGTCTTCTGAATGGGATTCTCTGATCCCTTGTC
TCAAGAGTAGGGCAGCCTAGAGAAGGGCACAGGGGATGCAGAGAGGAGGGTGTGACCCTGTGAGAGAAAT
GCCCTCTGTCCCCAAGGACTTTGAATCAGCCCCAAGCCTCTGTCCTCACAGTCTTCCATGGTGTGCCCTG
TGCAGGTCTTTGACGCTCGGAACTGTAGCACAGCACAGGAAATGTTTCAGCACATCTGCAGACACATACT
TTATGCCACCAACAATGGCAACATCAGGTGAGGCACCTTTCCCAGCTGTGCTGGGCCCAACTGACTACTC
ACTCTCAGGAAGAAGATGCTGGAGAGCACAGGAGAGGGGCCTCTGGCTCCAGGAGAAACTAAGGAGAGAG
AAAAGAATCCAAGACTTAGCTTTTCCAGGACATGAAATGGCAGGGCAGCCTCCTCCCCATTAGTCTCTGT
TATAGCCCAGCTCTTCCCCGACCCCTTTCTAAGTTAGAACCTCAGGGCTGTTACATCTTTAGGAAATTAC
TTTGAGCAGGCCATGGTGACACATGCCTGTTATCCAAGCACTTGGAAAGGTGGAAGCAGAAGGATCAAGG
GTTCAAAGCCATCCTTGGCTACATAGCACGTTCAAGGCTAGCCTGGGCTCCATGTGTTCCTGCCTTAAAA
ACAAAATAAAACCAAAACAAAGAAAACCATTTTCTTAGTTGGGCATGGTGGTACATAACAGCAATTTCAG
CAGTGGAAGGCTGAGGCAGGAGGATTACCATGAGTTCCAGGCCAGTCTGGGTCACATTGACAGACCTTGT
CTTGTTTTTAAAAAGAATCTACTCAGAAGGCAGAGGCGGGTGGATCTCTGTGAGTTCAAGGCTAGCCTAG
TCTATATAGTGCTCAGTCTGTCATTCACTGTCATGCAAAAAACACGCACCTAATGGGGATTCAGCACAGG
ACTAGGCCAGGGGCCAGGGAAAAGGAGGTGACAGGGTGGGATACAGAGGCGACTGAGACCCTAGAATGCA
GCTCCATCCAGCTGCAAACCTATGGTGCACAGCCACCAAGGGTGAGAATAAAAGGATCCAGCAGCGAAAG
CCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAGGGACATGAGGCTTCC
CAGAGTGGTGGCCCCTTGTTGGTCTTGATAGTTAGGGAGAAGCTGGCAGGAGGAGGAAGAGGAGGGTGAT
ATCAGGGTGATTGGGATGCAGGGAAGAGTGGAGGGAAGTTGGCTGGGAACCCCTGACCCACTCACCCCAA
CCTGGGACAGTTGATAAACTACTCTGTCCCTGTTCTGGGGAAGGTCGGCCATCACTGTGTTCCCCCAGCG
GAGTGACGGCAAACATGACTTCAGGCTCTGGAATTCACAGCTCATCCGGTACGCTGGCTACCAGATGCCC
GATGGCACCATCAGAGGGGATGCTGCCACCTTGGAGTTCACCCAGGTACTGACTCAGCCTCTCCTAGATC
CCTGTGGGCAGGGGACCCTGAGCAAATGTGGGGCCTGGCCCTGCAGACTTACTGGGCCCCTCTCTCCCTC
AGTTGTGCATCGACCTAGGCTGGAAGCCCCGCTATGGCCGCTTTGATGTGCTGCCTCTGGTCTTGCAAGC
TGATGGTCAAGATCCAGAGGTCTTTGAAATCCCTCCTGATCTTGTGTTGGAGGTGACCATGGAGCATCCC
AAGTAAGTCACCCAGGGTACGGGTTATATGCATGCAAGCCCATCTCAGGCAGCACCCACAGGACAGATGC
TGCAAGGCCTCTTTGCCCTCTGCAGCAGCTCTGGCCTTTCCTCTCCAGCCTCGCTTTATGTGGAACTGCT
GATGGTTACCAAGGGAACAGCAGCCTAGCAAACAGGGTCCTGGGAGAGTGGGATGCAGTGACTTAAGGAA
AGCCCCTGAGCTTCTGTCTGAGCAGAACCAGGCTTTTTTCTCACCTCCCAACAACTTCTTTAATTTTTTT
CTTTTATTTATTTAGTGTGTGTATTTGAATGTGTGTGCTTGTGTGTGTACCTGTATGTGACTGTATGTGT
GCATAGGTGTGCATGTTTGTGTGTGTGTGTATATGATATGTGAGGTATATGTGGTATGTTTATAATCATG
TGTATGTTGTTTGTATGTATGGTGTGTGGTGTATGTTTATGTTTGTAAGTGTGGTGTGCTTGTATGTTTC
TGTGTGTGATGTGTATGTGTGGTTATGTGTGTGTGTGTGTGTATGGTGTGTGTGTGTGTGCTCAGAGGTC
ACTTGCAGGAGTCAGTTGATTTGCTCCTACCATATGGGTCCTAGGGACTGAACTCAGCTTATCTATCAGA
CATGGTAGCTAGTGCCCTTACCTGCTGAGCCACCTCAATGGCCTCCTCCCAGCTTTCTTAGGAAAAGGAC
AACCCTCCCAGACAGAGGAAGTGTAGGCTCACAAGGACTTTAAGAGTGCAGCTGGGCCAGGCAGTGGTGG
CGCACGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCGAGGCCAGCCTGGTC
TACAGAGCGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTTGGAAAACAAACAAACAAACA
AACAAACAAACACGAGTGCAGCTGGTTGAACCTCTGCAAAATGCCTCCATATGTGGAGGTTTACCAGCTA
GCAGGTACTGACAGCTCCTGTCCTTTGAACACACTGAACTCAGTCCTGCCTCAGGACCTCTGTCCTGCTT
CCCTGCCTGCTATGCCCTTTCCTCGGAATTCTGCAAGGCTCCAGTTTTCCCATCAAAAGGTATTCACTAT
GACATCACTGCACCTCGGAGAAGTCTCTCCTGATCTCCTATCTTCAACCCCACACCACACTTCACTTCGT
TTCTCATTTTCTCACTGGTGTATTTATTTACCCCTCATAGTCTATAGTCAAACGGTCTATAGTCTGGATT
CTGGGCTGCAACTTTGGGAATAGAATCTTACTTTTTATCTATAAAGTGCAGGGCACGTAGTAGGTGCTTA
ATTATAGATATTTAGAATGTCTAACTATACAATGAATGATACTATGTTTGCAATAATCCCATGAGTTTGT
TGTTATCGACTCTGTGTCTATGATGGAGATACTGAGGCTCCGAGGGGCTAAAAGGACCCTGGTCAGTGCT
TGAACCCCAGCTGTCTGGGTGCCAGAGTACTTTGCTGCTACATTATGTTGGAGTCTCAGCATTTAGTCCG
ATATACACCTGACTTATTAGCATGAATGAGTGAGTAAAGAGATGTCTTCTACAATAAATGACCCTAAGTG
TTCCCTGTTATCTCTCGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGACT
GAGATCATCTCTTCAAAAGGACTCTCTAGAGCTCGGGCTATGGCTCAGCTGAGCATGATTGCTTAGCATG
CTTGAAGCCCTAGCTTTGCTCCCCAGCAGTGCATAAACTGGGTGTCGGTGATACATGCCTATAATTCAGG
CCCTTGGGAGATAGAATCAGGAGGATCAAAAGTTCAAGGCCATCCTCAGGTACAGAAAATCAAGACCAGT
CTTGGAAATGAGACCCTGTCTCAAAACAACAAAGAAAAAGAAAAGGAAGGATTTCTTTGGTTGATCCTTT
TACTGAAGTTGATTTTTTTTGATTGGGGAGAGAGGGCAATAAAGATGGCCCTAAGTGCAGCTGGCTCTGC
CTCCCTGCCAGGTACGAGTGGTTCCAGGAGCTCGGGTTGAAGTGGTATGCACTGCCTGCCGTGGCCAACA
TGCTACTGGAGGTGGGTGGCCTCGAATTCCCAGCCTGCCCCTTCAATGGTTGGTACATGGGCACCGAGAT
TGGAGTTCGAGACTTCTGTGACACACAGCGCTACAACATCCTGGAGGTGATGAGGGTTACCAAGCTGGGA
CAGCTGGAGGATCAGGATGGGGGACCCACTCCACCCAAACTGCCTCACCCTCATCACTTGGCTTCTATAG
GAAGTGGGCCGAAGGATGGGCCTGGAGACCCACACACTGGCCTCCCTCTGGAAAGACCGGGCTGTCACGG
AGATCAATGTGGCTGTGCTCCATAGTTTCCAGGTATGCCAGGTATCTTGGGGGTTGGTAGAGGTTTTGGC
AAGAAGGTCACTTGATCCAGGTACTTGACTGGCACTTGATGTCCCCTTTACAACATCATTCATTCATGTG
TGCATTCATTCATGCTTGCTGGGTGCTGAGACCCAGCATTATTGCTGGTCTAGCTCTGGGCAACTCCTGT
CCTCCCAAGGCTGCCTGCTCCCTGCTCCTGCACTTGAGCACTGTAGGCAGCTTGGCTTCCTTTGCTCCAG
GACATTGCTTAGAGACCCCTCCTCCCGGCTGCATCTCCAGGGCCTTCCTCCCTGCCCCATCATTCCCACT
GTGCACCAACAGTTCCTGCCAACCTCTGGCTCCTTCTTCTTCTCCTGCTCCTGTTACAGCCAATGAGACC
CATCTCCTCACACAGCCTCTTCCTCCGCCACTTGCAGCCTCCTTTCTGACTCCCTGGGACATCTGAACTT
CTTGCAGGGATCAAAGACTCAGGAAAAAAAGGGATCCTAACACAGGACCATAGCTCTCCTAAGCACATGC
CATGGTGTGTGTAAAGGTCAGAGGACAGCTAACAGGACCCCGTTCTCTGCTTTCAGTATATGGGTTCTAG
GGATCAAACCCAACTTGACTGGCTTGGCAGCAAGCACTTTTACTCCTGAGCCATCTCGCTGGCCCCCTTA
CTTTTCCTCCCACCCCGTTTCTTCTTCTTTTAAATCACCCCACGACCAGGTCCTTCCCTCCTTGAAGGCT
TCAGTCGTTCATGTCCTTAAAGAACCTCTCCTGGACCAGGCCACTTTGGGCAGTGCTCCCCCGTCCCCCA
CACTGTCTTCTTTCCTTCCAACTCCCTTAACTTGACTTTGTTGAAGTGGTTCCCAGCACCAGGTCGTAGG
TTGACGACCCCAATCTTTGTTGTTTCTTTCCTCCGCTGGAAATGTGAGCTGCGCAGTGCTCAGTGCTCCC
TGTGGCCGGGATTGTGACTGAGTTCACTGCTACACCTCTAGTCTAGCTCATGATGGAGGATTGGGAATTA
TGGAATGGATGGACAAGTGGAGGTGAGCCGCCCCCGCCCCCCCCACACACACACATGAGCAGGGGCCTTG
TGTGCTAGGTGGACATGGTAGCTGGTTCCTGTGTGAACTGAGCCTTCTCTCCTCAGCTTTGCTTCTGTGG
TTCTGTTTCCTGTGGAGCCTGGATATGTGAAGAGGTGGGGAGTCCAGTCAGGGTCATTCATTCTTTCAAC
GGGGATTTAACAAATGTTTGCCCCATGTGTGCCATGCATTAGCTATGGGTTCATACAATGTCCTCCAGGA
CTGGGACACTCTCTGGAAGATTAATTTTGTGCTCACTGGAGTAAAGGGTGGTGAGCTAACACAAAGAAGG
AAGAGCCCCACCCCCAGCCCCTGGGGCAGGCCCAGGCAGGAAGGACCACCCCTGGTTCATACATCCTAAC
CCCCAGGGACTCCCACCGCCAACCCTGACTCAAGCAGGATGCAGATGTGTCCCAGCAAATGCACAGAGAC
ATAGTCACCCCCTGATGATGTCCCTCTACTGCCCAAGTCTCCAGGCCATTCTTGTCACCAACTTCTAGGA
CACCCTTCAAAAAGAGATGGCCTTTTTCCCTTATAAAGTGAGGGCAGTAGACAGGTGTGATGGAGCACTA
CTGTAAGTCTAGCATTCAGGAGGCGGATGCAAGAGGATGGTGAGTTCAAGGCCAGCCTGGGCAACTCCAT
GAGTTATTTCTCAAAGAGGGAAACAAAACAAAAAATGGGAATAGCGCTTCCCTCGATGAGTCATTGCTAA
GATTAACTGAACGTGAGACACCTTTGGAGCTCTCTTGTTCCCGAGGGGTGCTCAGCATACGTGGTTGGTC
CATCTATGCTTGTCGGAGTTATCATCCTCTCCTGACATCCAAGGGCACTGTATATGTGCACAGATAGTAC
CTGGCTTACGAAGGCTCCACTTGGGAATTTTTTACTTTACAAGGAAGGATATGAAGGCACCGTGAATCTA
GAGCAACTCTACTTGGAGTTTTTAAAATTCTTTTTTTTTTTCATTTTATTTTATGTGTATGGATGTTTTG
CCTGCACCACATGTGTGTAGTACCTGGCCAGAAGAGGGCATTATATCCTCTAGGACTGGAGTTAGAGGAT
TGTGAGCTGCCATGTGGTTGCTGGGAATCAAACCAAGATCTTCTGGAAGAACAGTCAGTGCTCTTAACCA
CTGAGCCATCCTTCCAGCCCCCTACTTGGCTTTGTTGTTGTTTGAGACCTTGACTTCTCTCTAAAACTCT
TTTTTTTTTTGGTTTTTTATTTTGAGACAAGGTTACCCAGGCTGATCTTCAGTCACTCTGTTGTCTAGGC
AGGCCTTAAACTCAAGGCAGCCTCCAGAGTCTCTGTGCTGATGGGCCTGTATGGTTAACCGCATAAGGAA
TTTTAATTTCGATCTTTCCTGGGCTAACGATGATGGCCGGATGCTCTTTTGAAGCTGGACAGCAGCAGCA
AGCTACAGCCTCAGCCAGCTATGGCAGCTACAAGGACTAGAAGCTGACACTATAGTGTGTTGTACAGTCA
AGCTACGATGGTTGGGAGGCAAAGTGCTTTATCAAAAAGACTTGAGATGGAGTCTCATGTACCCTAGGCT
GGCCTCACACCGGCTATGTAACCTCTGATCTTTTTGTCTACCTCCGTAGTGCTGGGATGACAGGTGTGAG
CTACCACATCTGAGTCATTTGTTTGTCTTTTTTTTAATGTAGTACTGGGCATTGAACCTCCAGGTCTTGG
TGCATGCTAGACCAGCAGGCTATGTTAGCTGAGCTACACCCCAGTCCCCATTCGATGTGTTCTTGACTGA
GGATACTTTCATCTTGTGATGGGCCCATCAGCATGGAGCCCTATCATGAGTCAAGCAGCAGCTGTATATG
AGTTTTAAAGTGTGTGGTTTGACTCATGAGCCTGACATCAGGTGGCAGCTGCCAACCTTGCCTCATCCTT
CCCCAGGAATATCCCTTCCTTGGCCAACTTGAAACTATTTCTTAAGACTCTTTTCAGCTGTCCTGACTCC
CACCTCTCCTCTAGACCTATCCCAGACACTAGGGTTTCCCCCTCTGTCCCCTCTCTGTCCCCTCTCTGTC
CCTTCCAAGCAGCCTTTGCTTGCCTCTGTCACAAATCCCACTAGGCCTCATGAAAATAGCTGATTTCCTT
CAGCCTTTCCAAGAGCCTTGCTGTTTGCTGGCAATACATGGAGACTGTCTCTCCTCTGCCCCATGGTCTT
TCTCAGTACCTGACAGATGAACAGGAGCAGATAAGACCCTTTGAGAGCACAGGATGCTTCAGTGTTGGTT
CTCTCCTTCCACTGTGTGAATTTCTGAGATGAAACTCAGGTTGGGCTTGGTGACCAACGCCCTTACCTAC
TGAGCCCTCTCCCCAGTTGTAAAGAAATTTCTCATGGGTGAATGGATCTTCAGACTTTGATGGGGCCGCT
ACTTTTACTGGATCATCCCAGGCTAGTTTAGGCCTCTAGTTTGTAAGATGTGAATGGCTTGTCTTCCCTA
CACTCCGGTCCCCACCGTCCCCACAAACCACTCTGGTGGTTTTGTGTTTGCTTGTTTTGGAGACAGAATC
TCTTGGAGCCCAGGCTGGCCTCAAACTCCCTACGTAGCTGAGGATGACCTAGAACTCCCAACCCTCATCC
TGTACACCAGAGTGATGAGATTATAGTTGTCTAGTGCTTCTTGTATGCCAGGCAAGCACTCCACCAAGTG
AGCCTCCTTTAAGTTTGTATTTTCTTTACCTGAAACAAGATGGTGCAGTGTGAGCTGCCCACACCTGAGG
CTCTCTGTCTGGTGGCGATGCATGGAGACCTGTGCATCGAATTTCTCGATGCCGCTTTTAGGGTAGTGGT
TCTCAACCTTCCTAATGCTGGGACCCTTTAATACACTTCCTCATGTTGTGGTGACCCCAACCATAGAGTT
ATTTTCATTACTACTTTATAACTATAATTTTGCTACTGTTTGAATCATAATGTAAATATCTAAAATGCAT
ATCTGATAAGTGACCCCCAAAGGAGGGGGAGGGAGAAGACACATAGGTTGAGAACGTGGGAACCTCTCAT
GTGACCGCAGGCTGGCTGCCATATGTGTGACCGTGGTGATGGCCTAGGTCTCTAAGTCTGTGGACTTGGA
CCTGTGTGTGTTTCTGCAGCAGTCAGTGAGAACATGAGGAGCAGGGGCAGAGTCATCGCGTAGATCACTG
GCCCTCCAGTGCGGCACTTCCCGCTCTCCTCTGCTGCTGCATCTCAGTCCACTCCATCTTCTTCTCCTTC
AGAAGCAGAATGTGACCATCATGGACCACCACACAGCCTCAGAGTCCTTCATGAAGCACATGCAGAATGA
GTACCGGGCCCGTGGAGGCTGCCCGGCAGACTGGATTTGGCTGGTCCCTCCAGTGTCTGGGAGCATCACC
CCTGTGTTCCACCAGGAGATGTTGAACTATGTCCTATCTCCATTCTACTACTACCAGGTCAGGGAGAAGC
CTCCACCCACCTGCCCATCCCCGCCCCACCTCCCATCTTATAGCTCAGCTGTGGAGACTCTGTGGTGTGA
GGACTCAGGGCTGTACATGCCAGCTGAGTGCCACCTTGTGCTCTCTAATCTAGTGTACCCAATAAAAAGA
TACTAAAAGCAGGTGGGCCTCTACCTTCTGTGACCATGACAAAGGAAGCCAAGTGCCCACTGCTGTGTGG
CTCATGTGGGGCCCAGGAAGCAGTACCCTGAGCTTTGGCTAACCCTTGGTGGAGTGGGCCTCTATGAGTG
CAGTAGAGGGTCCTCTGCCTCTCCAGCATCCCCCGGCCCAAGCCCAGCCAGAACCCCACCCCCAACTCTC
CTTCCTTTCTGCAGATCGAGCCCTGGAAGACCCACATCTGGCAGAATGAGAAGCTGAGGCCCAGGAGGAG
AGAGATCCGATTTAGAGTCTTGGTGAAGTATGTGGAAGTGGGCATGTCCCTCTGTCTGTGCTGTGTCCCT
TTTACTGAGGCATCCTTCCTGACTCCACCATGAAGGGCAGGGCATACGGAGAATGTCCCTTTTGGTGTTG
GCTCCCAGAACTCTAGACACCAATTCTGAGACTGATTTGCTTCTGTAGTTCAGATTTGCCAAAGAGACTG
AGTCAGGATGCACAGGGGCCACCAGTCAGAAAATGAGAAGTAGAGGTTCTGCCCCCCACCCCAGGGATAC
CCCTGTTCCGTCTCTTCGGGACACTAACTCACCACTCAGCTGTCTGTGTATCTGTCTGTTCATCCCCACA
GAGTGGTGTTCTTTGCTTCCATGCTAATGCGAAAGGTCATGGCTTCACGGGTCAGAGCCACAGTCCTCTT
TGCTACTGAGACAGGGAAGTCTGAAGCACTAGCCAGGGACCTGGCCACCTTGTTCAGCTACGCCTTCAAC
ACCAAGGTACCCACTTAGGTGGAAGGAGTCTGGAGTGAGTGTAAGCGGCCCCTTGGGAGCTGGGGGCTGA
TGTTCAACGGTTGTCACACAGGTTGTCTGCATGGACCAGTATAAGGCAAGCACCTTGGAAGAGGAGCAAC
TACTGCTGGTGGTGACAAGCACATTTGGGAATGGAGACTGTCCCAGCAATGGGCAGGTGGGTGGCCGGAG
ACTCGGCAGAGAGGCTTGGGAGGACCAAGGAAAGGGTCCGGGGGACTCAGCATGACACTCTGGGTTCTTA
TGCCACTTTCCCAATGGCACACAGTGTTTTCCTGTGAATTGACCAACTCTTACTTGTATGTGTTTAGATT
ACTTGAGGGGATTCATTACATTTATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGCAAGTCCCACAGCTTGCATGTAGAGGTTAGAGGACAACTTGAAGGAGACAATTCTTACCTTCTGTAAC
ATGAGTCCCTGAGTTTTTCAGGCTTGGTGGCACTTTTACCTCCTGAGCCATCACTGCAGCCACAACCTAC
TTAAAAAAATTTTTTTTCCAAAACTTCTTATTTAACTGGGCATTGTGATGTACACTCGCAATCCCAGCAC
TAGGCAGTAGAGGCAGGAGGATCAGAAATTTGGGCGCAGCCTGGACTCCATAGGAAATCTTGACTCAAAT
TCTCCCTCAACCAGGATTCCTCCTTAGGGGCTGAGAGATGGCTCAGCAGGTAAGATGGATTGTGGCACTT
TCAAAGAACCACAGTCCAGTCCCAGCACCTATGTCAGGTGGCTCATAGTCTCCTATAACTTCAGCTCTAG
GGACTCCAACATTGTCTTCTGGCCTTCCTAGGCACTCACACTCTGTACATAGACACAATCTCTGACACAA
ATCTTTTTTGGGGGGGGGAGGGGTAAGGTTTGTTTGTTTGTTTATTTATTTAATGTAAGTGAGTACACTG
TACAGATGGTTGTGAGCCTTCACGTGGTTGTTGGGAATTGAATTTTTAGGACCTATGCTCGCTCCAGTCA
GCCCCCCTCACTCTGGCTCAAAGATTTATGTATTATTATATCTAAGTACACTGTAGCTGTCTTCAGACAC
ACCAGAAGAGGGCGTCAGATCTCATTACGGGTGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTC
AAGATCTGAGGAAGAACCCTTATTAAATTGCTCTTACCTTCTGAGCCACCTCACCAGCCCGACATAAATC
CTTTAAAAAGAATTTTTTTTTAAATGTATTGTGGCTAGAGAGATGGCTTAGCTATTAAGAGCACTGACTG
CTATTCCAGAGGTCCCGAGTTCAATTCCCAGCAACCACATGATGGCTCACAACTCACAATGTAATAAGAT
CTGTTGCCCTCTCCTGGTGTGTCTGAAGACAGCTACAGTGTACTCACATACATAAAATAAATCTTAACAA
ATGAGCAAAGACCGTATCATTCTCACAAAGGAAAAATGGGCCGAATATATGAATTTTGTCTAGCTACCCT
CATGCAGGCCTGCACAGCCTTGTGTTTTGAGCAGAGACAAGTATGAGCAAGATGTTAAGGCTTGGTTAGG
ACTAATTTGAGTTAGTCTCTCCTGTGTTGGAATGATGCAAAGGCCATCGAAAAGAAAGTGCATGAGAAGA
GATTACAAAAGGCTTAGCTTCCCACAGTGTGATGAGTCCCACACTGCCTGAGACAGTTTCCTGGAAAATC
AAAGTTATTTTAGAGAATCTGTTGTTTCTAGCAACTTCCAAGCAAGAAGCAAGGTTCGTCTTCTTTTTGA
GGAACCTTGTGTTTTCCAAAGCTAATTGGGGAGGCTAGGGACTTCTGGGTCTTCCATTGAAGGGAAGGGC
CCACGGGAGCTGGTTCAGCTGCAGTGAGGGGCAATCTGGACTTGTCCATTGGCTTTCCCTTAGTATCTGG
ACTCTTTGCCTGCTCAGTGTGGCCAGTGTTTACACAGACCATCATAGTAAAGGAAAGCAGTGTTTTTGTA
GCTCCTGGCCTGAGATCTCCGCTTCCTTGTCTTTTGTAGACTCTGAAGAAATCTCTGTTCATGCTTAGAG
AACTCAACCACACCTTCAGGTGAGGCATCTCCCATATCCCTGACTCCTGGGCCCCAGTCTGGGATCAGAC
CAAGGAACAGGGTGTACACAGGATGTCTTCTCAGACATGTCCAGGGGCAGACAGAGTTCCGGTCTGAGGT
TTGTCTTCTGAGTGGGTTGAGGAGCAAGATGTGGAAACTGAGTCCCTCTTATAGATTTGCAGGCCTAGAG
CCCACTGGTCACATGACCAAGGAAGGCCAGACTACCGTGTGACTCAGTGGGCATGGGTCTGTCCTTGCAA
ACCATGGGCATTGATGCTGGGCATCCTGAGTCTCCATGTGTGTCCTTCTTGGCAGGTATGCTGTGTTTGG
CCTTGGCTCCAGCATGTACCCTCAGTTCTGCGCCTTTGCTCATGACATCGACCAGAAGCTGTCCCACCTG
GGAGCCTCTCAGCTTGCCCCAACAGGAGAAGGGGACGAACTCAGTGGGCAGGAGGATGCCTTCCGCAGCT
GGGCTGTACAAACCTTCCGGGTGAGACAGGCTTTAATGAGTCTATGCCAAACACATCTGGGCCTGGTGTC
CTGGGTGTCATGGCCTCTGCTAGGTGTAGGGCGGGGGTATGGGCCTGGCATCTCAGGTCAAAGCTGCTTC
CCCAACCCTCACCCCACCAAGAAAAATGTCACTCTGGTGTTCTGCTTCAGGTGGGAGGTGATAGCTGTCC
AGCTCCGGCCCTGACCTTATGTCTCCAGGGCTCTGAAAGCTTGGCCACCTCGTAGTAGTGAAGTCTTCCT
TCAGTCTTCCAGCTCTGCAGAGGAAGGGACACAGGACACACTGCTGAGCTCTCGTGACCTGACTTTATCT
TCTCAAATCTTTACTCTCTGGGTTCCTCCCTGCTGGTAAAGGAAGCTTAACACTGAAATGAATGGACAGC
CATGGGAATCCCAGCAGACTTGATTCCAAGACTCAGGCCTCCTCTATGAGCTTTTCTATCAGGATAGGGA
AACCCCAGACATCCCTTGCTATGTGGGAAGGGATAAATATAAGATGACAGCTTTACTATCCAGGAAAATA
TCACTAACCACTGTGTGCCAAGCCTAGGATTCTCTGAGCGCCTCCAGCCCCTTTCAGATGGCACGTTGTG
ATTCTGTGATGCCGCCATTCGGTATTTGACCCATGGTGAGGGGTGGTGACGGTGACGATGAATGCCATTC
TGCCTAACTTTCCAGGCAGCCTGTGAGACCTTTGATGTCCGAAGCAAACATCACATTCAGATCCCGAAAC
GCTTCACTTCCAATGCAACATGGGAGCCACAGCAATATAGGCTCATCCAGAGCCCGGAGCCTTTAGACCT
CAACAGAGGTAAGAGCCCCTCTGCACCCTACTGGGCCTTCCCTGCTTGAGCTGAGGCTATCTGAGCCCTG
AACCAGTAGTCAAAGAATCCACCATGCCCTTCTAATGAAGGCAGTCACCTGGAACTGCCAGGCAGCTGTC
TCCCTGGCCACAGGGTCTGTTGGTACAGAGCTTCAGGCTCTGAGGGAGAGCGGGTCAGGAGCCCTGAGAG
AGTGGTAGTGGGCACAGGGAGCTGGCATCAGCAGGTGGGTGGTGGCGATAGCTGTGCCAGGCACTGTGCC
GGCCTCTTGGCCCACATGAACTGCCCACCAAGGGCTAAGGTAGCATGCAGGAATGCTCAGCTCAGCGTCT
GGGGCTTTCCGCAGCACAGCACAGGAGATCTGTACCATTTCTGTCTGTCTTTCCAGCCCTCAGCAGCATC
CATGCAAAGAACGTGTTTACCATGAGGCTGAAATCCCAGCAGAATCTGCAGAGTGAAAAGTCCAGGTAAG
GATGGGCAGGGATGCTTAGCTGGTACCCAAGATTGCCCGCGAGATGCTTCTTGTGACAGAAGTGTGGCCA
AGTACTCCATGCTCCCTGCACTGCTAGGGAAGGCTATAGCCAAACTCCTCTGGGAAACTCCAGAGTGCTG
AGGGTCCCCAGCTGAGGGGATGGCTTGTCACAAGCTGGCTGGTGACCACACTTGGTGGTGGGAGAACACT
TATCCTGGGGTAACTGGCAGTCACCCATGGCTCCCTTTTCTTCTGTAGCCGCACCACCCTCCTCGTTCAG
CTCACCTTCGAGGGCAGCCGAGGGCCCAGCTACCTGCCTGGGGAACACCTGGGGATCTTCCCAGGCAACC
AGACCGCCCTGGTGCAGGGAATCTTGGAGCGAGTTGTGGATTGTCCTACACCACACCAAACTGTGTGCCT
GGAGGTTCTGGATGAGAGCGGTGAGCCCCCGGCCCTGGACAAATCTCTACTTCCCTCAGTCCCATCCCTG
AGAACTGGGCCTGTGTCTGTCCTGATCTACTTCCATCCCAACTCATGGGAGAAGCTTTCTGGCCTTCACT
GGTAGTGAGGCCTCCCCCTAGCCTTGGACGAGGCACTGGAGGAGCAAAACCTGGCAGTGTCCATCATCAT
CCAGGAGAAAGGGAGTCAGCCTGAAACTTAGAGACAAGAGACTTGCATCCTTGGGGCTGTGAGAATACAG
ATGTGGTCCAGATGAGATGCATTAATAATAAAGTCCTTGTCTCTAGAAGGAAGTGAGGGGAGTGGAGAGG
AGTTAAAAGCAATGTGCTAACACGCACTAAACGTGCCCAGGGAACGGGCACTGTGCCAGCGATTTCCCAA
ATGTAGTTTTTACTCATTGCCAGCACAGACTATGTCATCACATCTGTTTCTCAAGAAGCAGCTGGAGGTC
ACAGGATTTGGACACTCGGCTGAGAAGTGGGAACCAGAGTTTGCGCCTGGGTCATGCCGTGACTCTCCGA
ATGCTGCCTCTTGGGACTCCTGAAGCCCAGCCACCACAATTTACATTGATCTGTTAATATCTCAGGAGGT
TGTAGCCATAAACCTCTCTCTAAAAAAGCAACCAGCCTCTGGGGCGTATATCAATCTCCAGTAGACTTAG
TCACTAAGTTTGAGGTTCACCTGGGGATGTCAGAGACTCAAGTATGGGCCACAGAAGGATTGCTTAGTCT
GTCAGGACAAAGCCAATATAGGTGCCCACGTGGGGGCAAATTTTCAGCCTACCCTGGCAGCCCTGTCTTG
GCACCAGCTCCTGATCTTTGCTCTTTCTGGAGCAAAGATGTCAGAGTCTTCCCTCCCTTGCAGATGGAGA
ATAGCCATCATCTGAGAGACCCAAGTAGGGCTTTCCCCACCCTGACACCTTTCTTTCTTCCATTCAGGCA
GCTACTGGGTCAAAGACAAGAGGCTGCCCCCCTGCTCACTCAGCCAAGCCCTCACCTACTTCCTGGACAT
TACGACCCCTCCCACCCAGCTGCAGCTCCACAAGCTGGCTCGCTTTGCCACGGACGAGACGGATAGGCAG
AGATTGGAGGCCTTGTGTCAGGTGAGGCAGGGGCAATTCAGTGGAAGGAATAGAGCCCAGAGCAGAGTTC
AACCTCTGAGACCATCCTCCTGAGTCCCTAGGTGTGCCTTGTCCGTGTCTGGTCGGGGCTAGGCGTCGTC
TTCTGCCCCGTCTCTAGTACACAGCAGTATTGAGTGAATGAGTTTCTCTGGGAATCAGTATGCTGTAACC
TGTATTCATCACATGTCCCATTTCTGTGACTAACTGCATGTCAGAAGGAACTTAAGGGAAGGAGGGTTCA
TTTTGGTTCATGAGGGTCCAGTCCATCATGGTAGGGTGTCATGACAACAGGAGCATGAGTGACAGATCAC
ATTGCTTCTGGAGTGAGAAAGCAGGAAAGGTGGACACCAGTGTCCTGCTCAAAGTCCCAGACCATAGGAA
TTCGGGGTGGGTCTTCCCAGTCAAACCTCTAGAGAAACACCTTCACCAAGGTGTGTTTCCACAGTGATCT
GAAATCCAGTCAGGTTCACTGTGAAGAGTAACAGCCAAAGTGCACTTCCTTAGAACCTGACTAAAGGGGG
AAGCTCTATGGATGGCCTTTGTCTTCCCAAGTGTGGACCACGCTTGGTGGTGGGTAAATGGGGGTACTGG
CGGGTGGGGAGCTGAGTTAATGAGTTAGTGGGGTGATGCATTAAGTCACTTGCGACTTTAGAGTCCATGT
CTTTATAAGAGTCACTGGACAGCAAGCCATGGGACACATTGTCATTGTCTCAACAATCCTATATTAATGG
CTGCATCCACCTAACTCCACAAGGATTAGGGTTAAGCTAAGGAGGGACATCCAGCCATGACTTGAACTCA
GATCCAGTTCGTTCCAAGTTACAGTATAATAGAGAAATTAGCCCAGCTGGGTTGGGTGGTGCATTCTTGT
GATCCCAGCACACAGGAGGCTGATGCAGGAGGATAGTGAGTTAGAGGCAAGTCTGGGTCAACAATGAGTT
CAAGACCAACCTGGGCTATGTAGCAAGACCATGTCTCTACATCCTCCCCAAAGAAAGAGGACACTGAGCC
CTGCCCATGAGTTGCTGATGTTGGGAGGCTTCTGTGTTTTGATTTTTGTCTTTGTTTTGTGGGTACTAGT
GTTTTCCTTCAGACCTGCACACATGCCAGGCTCTACCACTGAGACAAACTCTCTCTTCTCTGACTTTTAA
TTCTGAGGCAGGGTCCCACTAAGTTATCCAAGCTAGCTCTCAATTCGGTCTCTAACCCCTGCTGGTCTTA
AACTTGGAATTCTCCTGCGTCAGCCTTCTAACTAGTTGGGGTTGCAATCCTGCCCCACTGTCATCTTAAT
TCTATTCATAGTAGAGTTCTAGGCACAATTGAGAAATGCTACTAGCTGCCCCAGGTCCTTGCTTCCCGTC
CACCGGAGTAGCCTCAGCACCCTGGATAGCGCCCGTCTTTTGCTGCGTTTGTTGAAGGAATGAATGGATA
CTGGGTGGGGCAGCTCCTACACGCACGCAGCTCCTACACGCACGCGCGCACGCACGGCAGGTCCTCCGAT
CCCTCACACTGCCCTTGCCTTTCAGCCCTCAGAGTACAATGACTGGAAGTTCAGCAACAACCCCACGTTC
CTGGAGGTGCTTGAAGAGTTCCCTTCCTTGCATGTGCCCGCTGCCTTCCTGCTGTCGCAGCTCCCTATCT
TGAAGCCCCGCTACTACTCCATCAGCTCCTCCCAGGACCACACCCCCTCGGAGGTTCACCTCACTGTGGC
CGTGGTCACCTACCGCACCCGAGGTGAGACAGGCAGGCTCTGGTGTCATCTGCACCTGTCCTTCTAGGGA
CAGCTGTCCTCGATTGCTTTCTGTTGCTGTGACCAACACTGACTGCCACCACTTTAGGGCAAGATCCAAT
TTAGGGGTTTAACTTAAAGTTTACAACTGTCACCAAAGGAAGCCAAGGCAGGAACCACAGAGGAACGTTG
CTTATTGGCTCCCTCTGGCTTGCTCAGCTGCCTTCCTTACCAGCTCAGCACCACCTGCCCAGGATCAGCA
CTGCTCACAGTGGGCGATGCTCTTCTCCATCATCAATTAGCAGTTAAGAACGCACCCCACAGACATGCCG
ATAGGCCTATCTGATGACAAGCCCTCAATTGAGGGTCCCTCTTCCCAGGTGTATCAAGTTGATGGCCGAC
ACTGGTCACCACAACAGCTGTTCAATACCTTGGGGCTTTGTCAGCTACATGCTGCAGTGACAGCCACCTG
CCAGAGTTGTCAGTGATATAAGATGCTGTGTAGGGCACAGTGTCACAAAGCAGGAGGGAAGCTCGCTCCT
TTGTTTACCATTCAGACCTGTAGGAGATGGCTTCCATGTGTAATTTTACGAGGAAGGAAAAGAGTCATGG
AGAGGTTAACCCACCTGTTCAGAGACACCTGTCATTAACTAGAGACACAAATTCAGGGAGTTTACCTGCT
GAAGCTAAGAACTGGACCATTGTTACAGTCTCTGGCTGTTGAATAGGCTCAGACGTGAAAAGCCTTGAAT
GCGTAGGGAACTCAGTTACTTTTGGTCATCCTAATGATGCAGGTGGATGCAGGGAACATTTTCTCAATTT
TTTTCATTCCCTCAGTGTTCCCATCCTGTGATGGCCGTGTCTCACCTCCATGCCAGGGGCCACACTGGGT
GTGGAAACTGCAGACAGGCCCTGCCCTCACAGAGCAGCCAGAAAGTCAAAAAAGAGTTCAGGCATGGACA
TACTTGGACATATGTTTGTGTGGGTGCTCAGAGGCCCCCGGTATCACGAGGGAGGGAGTGGGGTGTGTGT
GAAGCTGCCCTAGACTAGGAGGTCAAGTGTTCTAGGCTTGGTTCCTCCACTGGATGGACCTCCCTGTTCT
AACCACTGTAACTGAGCTCTGTTTGAGCTGGAAGGGCTGGAAAGCGTCCACATCTCCGAGATCTCTCTCC
TTGGCCAAGAGCGCAGTTTGTAAATACTGCACAGAGCCTGTGTCTGTCCATCCTCACTGACCATACCACA
CCATTGAATGAGAGAGGAGATGCGGGTGAGCCCTGACAAGGCTGGTTCATGCAGACCAGAACCAAACAAC
TATTCCAGTTGCTAAAGGCCTCCCTTATGTGTCAGGCCATGGGGAAGTGAATGTAACCCAGAGGACACTG
GGGTACCCACAAGCCCAGGAAAAGGCTTCTGGGGAAGATGGAGATGCCAGAATAGACAGGGGGCGTGGAA
GACCACCCCTCACCCAGAGTTCCTATTGGCAAAGGCCTCCTTCATGAGGACACTCAGCAATGGCCAGCCC
TGGGGATCATTTCACACTGCGTGGGCAGAGCCTTAGTGGGGAAAGGATGTTGGGAGGGTCCCTAGACAAG
CACTTGGGAGATGCTCTAGCGTAAGACTGAGGAACCTGAGCAGGAAGTTGAGTCCACCATGACTTTAGTC
CCTTGCTCCGTTGCCTTCTGTGTGACTCAGTTTCTACAATGGCAAAGGCGCAGTTGCTCACCAGGCCCCT
GACTGCCTTCCCTGATCTGTATAGACAGCTGGGAGAAGAGGTAACTCCTCATTCAGGAAGCAGCCACCCA
ACGGGGCTTGACACTGCAGGAAGAACACCCAGCCGGGTTGCACGAAGCCCACGGGGCTTGTTAGTCAGGC
CTGTGTTTTCCTTGCAGATGGTCAGGGTCCCCTGCACCATGGTGTCTGCAGCACTTGGATCAGGAACCTG
AAGCCCCAGGACCCAGTGCCCTGCTTTGTGCGAAGGTAAGCACCCACTCGGGGCCCTTTGGAGCCCCTAG
CCCCTGGGGATTCTGCAATGCAAGGGAAGGGTCTAAGACTGAGTCTCCTCTCGGCCCATAAAGGTTTAGG
GGGTAATTCTTTGTCTCCTCGTTTTGCCATCTGGTTACAAGGTGTCGTGGAGTCCATGACACAGATACAG
TGATGCCTGTGGTGGGATCCAATGAGACTGGCCCAGTGTGGAGAGAGGCCGTTGCCTGGGCTGGGAAGAT
GAAGATGTGCACAGGTCCCCGCCAGGCGCTTAGGTCCCCAGCCCTATCCTGCATGTGCTCCTAGCCTGGG
ATATCTGCAGACCAGGGTCTTTCTCCTTCTGTCTTCCTGCAGTGTCAGTGGCTTCCAGCTCCCTGAGGAC
CCCTCCCAGCCTTGCATCCTCATTGGGCCTGGTACGGGCATTGCTCCCTTCCGAAGTTTCTGGCAGCAGC
GGCTCCATGACTCCCAGCACAAAGGTACAGCTCTTACTCAATAGGGAGTGTGCCTGTCACTGTGACCGAC
CTGATCATTACACAGGCACATCAAATTACTACACCATATCCCATAATCAGGTACACAGATGTGGAGTGTT
TGTGCTCTGCCCACTCCACGTCTCCATGTTTCCTTGTTAATTTTTAAGGTATCTAGAGGAGTGTGTATGT
CCTGGTACGCATGTGGAGGTCAGAGGTCAACTTGTTTTACTTCTTCCAGGAATCAGATTCAGCTTGTCAG
TCACGACGGCAAGCACCTCTCCCTGCTGAGCCATCTCATCAGCCCATTTTGCTGGGTTTTAAATGCTTGC
CTCCTTGCTGGACCATCCATTAATAACACAGAGATTAATAAGCCTGTGTAGGGGTCCCCAGAGTGAAGGG
GGAATGAGAGTAACAGACTTTAGATGAAGACATTTTCTTTTCATTGGAGAAATAGCTGGGAAGGATGGAC
AGCTCTGTCTGGAATCCTCCAGATGAGGCTTTTCTGGAAGAAACCCTTAGCAATGTTTGACCTTCAAGAT
GCCTGAGGCCTCTGCACCTATATTTGATGTAGCATCTGGGTCAGGGGAGATGATTTTCACAAAAGAAGGC
CACGAGTTTCTGATATTCCACTAAAAGAATGTCCTTAGCATGTTACACACACACACACCTATACATATTT
GAGACAAAGTCTTGCTATTGTAGCCCTGGATGAACTGAAAATTCCCTATACAGAGGAGGCTGTCCTCAAA
TTTGCAGGAAATCAATCCTCCTGCTTCTAATTCCCAAGAACTGGCTATAATAATATTTTAAAAGAAGAAG
TAAGATTAAAATAGAATATGTCAAGTAAAAGAAATAGCAATGTGTAGACATAATCAGAACTGATACTTTT
TTTTCCTGAACCATGTTTTTTAAGAGTGCCACCATTAGCTATAAAATGGGAATAATGCCAGATCAGAAAC
CTACCCATTGGGTACCTGGTGCTCACAGGTTGCCCTCTGGTGGCCGTTGTGGTAACTGCAACCTTCTGGA
TCTTCAAGGGCTCAAAGGAGGCCGCATGAGCTTGGTGTTTGGGTGCCGGCACCCGGAGGAGGACCACCTC
TATCAGGAAGAAATGCAGGAGATGGTCCGCAAGAGAGTGCTGTTCCAGGTGCACACAGGCTACTCCCGGC
TGCCCGGCAAACCCAAGGTAAGTGCAGCCACCCAGCAAGCTGGCAGAGCACGCATGATGGCCCCTGCCCA
GGCTATACAGCCCTTCCACTCCAAGCCTGCAGTGAAGACAGTTGCACAGTGGCAGGGAGAGATGAAGAGC
ACGATGACAACATCAAGCTCACTGTAGTTTAATCTCCACAGTGCAAACACCTGCAGGTGCCCAGCATCTC
TGGGACGCAGGGCTGGGCCGGGCTGGCTTTACACTTAGTTCAGTAAGACTGGAAGGTGGAGCTGATTCCC
CCCAGGGCCCTCAGGTTGGCACTCTGCTCCAGGGGCATGAACAGTGCCTACACACTTTCCATTCAGGACC
ATGTGAACATTCCCTGAAGACCCCATTTGCATCCTAAAGCTGGACCACTGGTCATCTTAGATGAAAAACA
AAAACAAACAGACTCCTGACTGACTGCAGGACCTTCTTTATAAATGGAAAGCCTAGGAGCCTATACCTGA
GCTACAAATCAGAGCTGGGGCTAATACATACTGGGAAGCCCCCTCTTTCCGGGGTGTAGACAGGCAGATC
TCACAGCCCTGGGCAGAGCTATAAACTTGATGTCTCTAAAGTATGGTGACCTGAGGGAAGCTTTACGGGT
TCCATTGGCCAGGAGGACAGGATACTTGTCTATCCTGAGCTGAGGTCCTGACTGAAGCCCTCAATGGCCC
TCTTCCCCTCCCCATGGCCAGTGTGCCTGACCACAGTGACACCAGCATCCAGGGCTGGTCCCACCGTGTC
CTCTGCTTTTAGGTCTACGTTCAGGACATCCTGCAAAAGCAGCTGGCCAATGAGGTACTCAGCGTGCTCC
ACGGGGAGCAGGGCCACCTCTACATTTGCGGAGATGTGCGCATGGCTCGGGATGTGGCTACCACATTGAA
GAAGCTGGTGGCCACCAAGCTGAACTTGAGCGAGGAGCAGGTGGAAGACTATTTCTTCCAGCTCAAGGTA
TGGGAGCTGGGGTGGGACCCTCCAGAGGGGAGTAGACCAAGGTCACAAGCCTTTGGAACTAGGACCAGGG
ATGCAGAACTGGGGTGGGGGAGGTGTGTGCCCTGAGATCCTCGGCACCACTGCAGAAACCTGGGGCCGGC
TGGGCTCCTCAAGCCTGGTCTTTCCATGCCATAGTGGTTGCCAGCTAGCTGAGAAATCAGGCCAGTGTGT
TCCATTTCTCTATATCTGGGATGAAAGCAGCAGTCTCTGCTCCCCACACTCCTGCCTAAATGCCCATTGA
GCATAACAGGGCTGTGGACTGTCCCCTACTGCCTATGGGGGACAGACACTTATTTACAATAGGTTGCTGG
CTAGGTGCCCTCATTTCTCACAGGAAGGACTCTTGGTAGCAGGGTCTGCCTTGTGCCAGGTGCTCACCAA
TGATTGCCCCGTGGTGACCTTTCCTTCTGCCCTTCCAAGGGCTTTATGACGGAAGAATGACTGTTCCATC
ATTTTGCAGATGGAAGGGGCCCTGGTGCCCTAACAGCGAATGTGAGAGCCCAGACACATGGCCCCTCTCT
CCTTTTCCCTCTCTCTGTCTCTCTGGTTCCTAAACCTGCCTTTAAGGCTCGAAGTTATGCTCCCCATTTA
TATTAAACATCTACTATGTGTTAGAGGTAAGAAGCAGTCAACAAAACAGGTGAAAATGTTGTCAATATCA
CGATAGATCACAGAGCACACCTGTATCGCCAGCACTGGGGAGGTGGAGGCCCAGGGGTCAAGAGTTCAGA
TTGTCCCTGGGTTTCATAGTCAGTCCAAGGCTGGACTGGGCTATATGAGATCCTATTTCGAAAGTACAAA
AGCAAAAGACCTACCTAAACGTAGCTTACATTCTGGCAGAGTTGATCAAATCAAGCACATAGGGTGCGCA
CTGCTTTGGGTAAGACGCAGAACAGCAGCTCAGAGCTGGGGTGTTTGTGGTTTAATGAGGTTTGGGGAAG
GGGTCCCCGGGAGGTGACATTTGAGAAAAATACCTGAAGAAGGAAGAAGCTTTGTGCACACTGGAGACAG
AGGTCCAGACTATTCTGTGGGGCAGCAACATGTTCAAAGGTCCTGGGGTGTGGACTATTCTGAAACCTGT
GATGACCAGCAAGGGTATCATGGGTACTTCCCAAAATGTGTTCAGAGCTTGCCAGATGTGTTCATGGATG
TTAGACATCATTAACAATGGGCTGTAAGGGCAGAGGCAGTGCACACCTGTTAGTCCTAAGGCTTTGGGGA
AGCTGAGGCAGGAGAGTTGTAAGTTCAAGTCAGTATGGGCTACCTTAGGCTGCAAGAATGCAGAGGACAT
ACCTGTAACATCAGCTCCAGGGGATCTGATCCCTCATATACAGGTACATGTATACACACACACACACACA
CACACACACTTGAAAATAAAACTGTAGCCAGACATGGAGGCATGTGCACACCTTTAACTCTAGCACTCAG
AGGCAGGAAATAAAATCCTTTCTTTTTTATTTATTTTAATTTAAAAAGAGAAAATTAGTTCCAGGGGTAT
AGCTTAGGCCTTGCCTAGCCTGGTATATAAAAGGCCCCAGGTTTGATTTCTAGTACCAAGGGAAAAAAAG
ATTCCAAGGCCAGACATTTGGTACACACGTGCGCACCCACAAAGAGCAACAGGTGTCTGCTGTAAGATTT
CTCTCGGAGCCCTCAGCCTGCTGCTGATTTCAAGGCCTGAAGAATGGGAGGCACACACTGCCTGCCCTGC
ACATCCTGATCTCAAAGCTCACATCCGATGTGGGATGAGCACTAGACAGGTAGTGGGACCGACATCCATC
CATGAAGTCTGGGAAGAGGCTGAGGTGGGCCACACATGGGCAGAGGAGAAACCAAGCATGGGTGTAATGG
CAGCAATCCAGACAAGGGGGTAGACACAGGGCTAGCGAGCGCCTGTGTCACCAGGAAGCTGTGGCAGCCC
TAGGGTTTTGTCCAAGCCTAAACATGATAACTGAGAGGTGGCTGGGCCAGCAGGGTGACACTGTCACCTT
TGTTTCCCCCTCACAGAGCCAGAAACGTTATCATGAAGATATCTTCGGTGCAGTCTTTTCCTATGGGGCA
AAAAAGGGCAGCGCCTTGGAGGAGCCCAAAGCCACGAGGCTCTGACAGCCCAGAGTTCCAGCTTCTGGCA
CTGAGTAAAGATAATGGTGAGGGGCTTGGGGAGACAGCGAAATGCAATCCCCCCCAAGCCCCTCATGTCA
TTCCCCCCTCCTCCACCCTACCAAGTAGTATTGTACTATTGTGGACTACTAAATCTCTCTCCTCTCCTCC
CTCCCCTCTCTCCCTTTCCTCCCTTCTTCTCCACTCCCCAGCTCCCTCCTTCTCCTTCTCCTCCTTTGCC
TCTCACTCTTCCTTGGAGCTGAGAGCAGAGAAAAACTCAACCTCCTGACTGAAGCACTTTGGGTGACCAC
CAGGAGGCACCATGCCGCCGCTCTAATACTTAGCTGCACTATGTACAGATATTTATACTTCATATT

Protein Sequence : Show Sequence

>gi|6754872|ref|NP_035057.1| nitric oxide synthase, inducible [Mus musculus]
MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLH
VTSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEF
INQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARN
CSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEF
TQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANML
LEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSF
QKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPW
KTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFN
TKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCA
FAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEP
QQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIF
PGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKL
ARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDH
TPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGI
APFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYV
QDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHED
IFGAVFSYGAKKGSALEEPKATRL

Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis guaB mutant vaccine , LVS

15. TNF-alpha

Gene Name : TNF-alpha
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 21926
NCBI Protein GI : 7305585
Genbank Accession : AB039224
Protein Accession : NP_038721
Taxonomy ID : 10090
Chromosome No : 17
Gene Starting Position : 35199389
Gene Ending Position : 35201995
Gene Strand (Orientation) : -
Protein Name : tumor necrosis factor
Protein pI : 4.75
Protein Weight : 24010.47
Protein Length : 235
Protein Note : Also known as DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434

DNA Sequence : Show Sequence

>gi|372099093:35199389-35201995 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
CTTTATTTCTCTCAATGACCCGTAGGGCGATTACAGTCACGGCTCCCGTGGGGAGCAGAGGTTCAGTGAT
GTAGCGACAGCCTGGTCACCAAATCAGCGTTATTAAGACAATTGGGTTAGATAAATATTTTGTTTTAAAC
ATAAGCAAAAGAGGAGGCAACAAGGTAGAGAGGCCAGGTGGGGACAGCTCAGCTCCGTTTTCACAGAAAA
CATGTCTGTCTGAAGACAGCTTCCCACACTGGGTCCTCCAGGACACCCCGGCCTTCCAAATAAATACATT
CATAAGCAAATAAATAAATAATAAATAAATAATAAATAATAAGTGCAAATATAAATAGAGGGGGGCTGGC
TCTGTGAGGAAGGCTGTGCATTGCACCTCAGGGAAGAATCTGGAAAGGTCTGAAGGTAGGAAGGCCTGAG
ATCTTATCCAGCCTCATTCTGAGACAGAGGCAACCTGACCACTCTCCCTTTGCAGAACTCAGGAATGGAC
ATTCGAGGCTCCAGTGAATTCGGAAAGCCCATTTGAGTCCTTGATGGTGGTGCATGAGAGGCCCACAGTC
CAGGTCACTGTCCCAGCATCTTGTGTTTCTGAGTAGTTGTTGAAAGCTCTGAGCACAGAGTTGGACCCTG
AGCCATAATCCCCTTTCTAAGTTAGAAGGATACAGACTGGGGGCTCTGAGGAGTAGACAATAAAGGGGTC
AGAGTAAAGGGGTCAGAGTGGGGGCTGGGTAGAGAATGGATGAACACCCATTCCCTTCACAGAGCAATGA
CTCCAAAGTAGACCTGCCCGGACTCCGCAAAGTCTAAGTACTTGGGCAGATTGACCTCAGCGCTGAGTTG
GTCCCCCTTCTCCAGCTGGAAGACTCCTCCCAGGTATATGGGCTCATACCAGGGTTTGAGCTCAGCCCCC
TCAGGGGTGTCCTTGGGGCAGGGGCTCTTGACGGCAGAGAGGAGGTTGACTTTCTCCTGGTATGAGATAG
CAAATCGGCTGACGGTGTGGGTGAGGAGCACGTAGTCGGGGCAGCCTTGTCCCTTGAAGAGAACCTGGGA
GTAGACAAGGTACAACCCATCGGCTGGCACCACTAGTTGGTTGTCTTTGAGATCCATGCCGTTGGCCAGG
AGGGCGTTGGCGCGCTGGCTCAGCCACTCCAGCTGCTCCTCCACTTGGTGGTTTGCTGAGGGGGGGGGGG
AGGATTGAGTCAGTGTCACCCTCTTAGTTCACACTCCACATCCTGAGCCTCAGCAGCTACCCACACTTCA
CTTCCGGTTCCTGCACCCTCTGTCTTTCCACATCCCATTGGCTATGAGGTCCCGGGTGGCCCCCTGATGC
CTTGCTTTTGAGTCACTGCTCTGACTCTCACGTGCTGTCTCTAAGAGCTCTGTCTTTTCTCAGCCTGGCT
CGACACCCCTCAACCCGCCCCCCAAAATCATGCCCCTTCATTCTCAAGGCACATGTAAAGAAATCTTACC
TACGACGTGGGCTACAGGCTTGTCACTCGAATTTTGAGAAGATGATCCTGGAGGGGAAGAGACAAAGGCA
AGGATGAGCCTTTTAGGCTTCCCAGCAAGCATCTATGCACTTAGACCCCTTTCCTCCCAAACCAAAGCTT
TAAGTTCTCCCCCCACCCCATCTCATCCCATGCCTAACTGCCCTTCCTCCATCTTAAATTAAGAGAGAGG
TGTGGGAACACTTACTGAGTGTGAGGGTCTGGGCCATAGAACTGATGAGAGGGAGGCCATTTGGGAACTT
CTGTGTAGGAAAAGGAGGTTAGTTAAGACAGACTCACCCCAAAGGAGAAGCCTCCCGGCTGATTGCCCCG
CTTACAGTTCCTCTTTGCCCCACCCCACCCCCCAGCTTTGTGTTTTTCTTCTTCATTCATTCATCTGTCC
AACCCACGGCTTCTTTCTGCGGTGCCCTCTGTGCTTGATCTCCCGTTATCTCCCCTTCATCTTCCTCCTT
ATCTCTCATGCCTCTCTCATTTCTGTCTCTGAGTTTTATCTCTTGCTTATCCCCTCTTCCCCTGGCCACA
TCTTTCCAGATCTCTCCACGTGTGAACACACTTGTTCGTTCATTCATCTCTCTGTGCATCCGACGAAGGA
TGTTTAGTCAGCTGGACGCATGGGTCCGAGGTCCTGACTCTGTCCCCTCCACACTCTCCTCCACCTTGCC
CTGCCCATTAGCCCACTTCTTTCCCTCACACTGTCCTTCTTGCCCTCCTAACCCGTTTTGCTTGTGAGCG
AGAATAAGGGTTGCCCAGACACTCACCTCATCCCTTTGGGGACCGATCACCCCGAAGTTCAGTAGACAGA
AGAGCGTGGTGGCCCCTGCCACAAGCAGGAATGAGAAGAGGCTGAGACATAGGCACCGCCTGGAGTTCTG
GAAGCCCCCCATCTTTTGGGGGAGTGCCTCTTCTGCCAGTTCCACGTCGCGGATCATGCTTTCTGTGCTC
ATGGTGTCTTTTCTGGAGGGAGATGTGGCGCCTTGGGCCAGTGAGTGAAAGGGACAGAACCTGCCTGGTT
GGCTGCTTGCTTTTCTGGGAGCTATTTCCAAGATGTTCTGGAGTTTCTGTTCTCCCTCCTGGCTAGTCCC
TTGCTGTCCTCGCTGAG

Protein Sequence : Show Sequence

>gi|7305585|ref|NP_038721.1| tumor necrosis factor [Mus musculus]
MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPL
ISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQ
VLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQL
SAEVNLPKYLDFAESGQVYFGVIAL

Molecule Role : Vaximmutor
Related Vaccine(s): Francisella tularensis FTL0552 mutant vaccine , Francisella tularensis guaB mutant vaccine

IV. Vaccine Information

1. DeltaFTT0918

a. Vaccine Ontology ID:

VO_0011346

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Antigen

DeltaFTT0918 is an attenuated SCHU S4 strain with mutation of the gene FTT0918 (Twine et al., 2005).

e. Immunization Route

Intradermal injection (i.d.)

2. F. tularensis FopB Protein Vaccine

a. Vaccine Ontology ID:

VO_0004034

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

.5 μg recombinant FopB protein

e. Gene Engineering of FopB

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Adjuvant: CpG DNA vaccine adjuvant

VO ID: VO_0001237
Description: 10 μg of CpG oligodeoxynucleotide (5′-TCCATGACGTTCCTGACGTT-3′; Operon, Huntsville, AL)

g. Immunization Route

intranasal immunization

h. Mouse Response

Host Strain: C57BL/6 and HLA-DR4 tg
Vaccination Protocol: mice were anesthetized i.n. with 3% isoflurane by use of a rodent anesthesia system and were immunized i.n. on day 0 with 7.5 μg rFopB in 25 μl of sterile PBS. This was accompanied by immunization with 10 μg of CpG oligodeoxynucleotide on days −1, 0, and +1. Mice were boosted once on day 14 with the same amount of antigen (Yu et al., 2010).
Challenge Protocol: Mice were challenged i.n. with approximately 3.5 × 10^4 CFU (∼5 50% lethal doses [LD50s]) of LVS on day 30 (Yu et al., 2010).
Efficacy: Intranasal vaccination of mice with rFopB conferred 50% protection (P < 0.05) against an otherwise lethal pulmonary challenge of 5 LD50s of LVS (3.5 × 10^4 CFU) in both C57BL/6 and HLA-DR4 tg mice compared with the protection conferred to LVS-challenged mock-vaccinated mice, all of which succumbed to the infection by day 10 (Yu et al., 2010).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: Spleens were collected at 14 days postvaccination, and single cells were prepared and assayed for FopB- and LVS-induced IFN-γ production. Differences in IFN-γ stimulation between rFopB- and mock-vaccinated mice were significant (Yu et al., 2010).
- Detailed Gene Information: Click here.

3. F. tularensis GroEL protein vaccine

a. Vaccine Ontology ID:

VO_0011446

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

F. tularensis chaperonin GroEL (Hsp60)

e. Gene Engineering of groEL

Type: Recombinant protein preparation
Description: Hsp60 was identified by Western blotting with a monoclonal antibody to GroEL (Sigma-Aldrich, Poole, United Kingdom). Hsp60 was excised from large-format unstained gels and electroeluted into 4× Laemmli buffer (0.1 M Tris-HCl [pH 7.3], 0.768 M glycine, 0.4% [wt/vol] SDS) by using the Hoefer gel eluter (Amersham Pharmacia, Buckinghamshire, United Kingdom) at 70 V for 2 h, following the manufacturer's instructions. The eluted product was pooled and purified by dilution (more than 20×) in ammonium bicarbonate (3.95 g/liter) and SDS (1 g/liter) and centrifuged over a dialysis membrane (10,000-molecular-weight cutoff; Vivascience, Lincoln, United Kingdom). The protein was then diluted (20×) in sterile water and centrifuged further, until significant concentration was achieved (Hartley et al., 2004).
Detailed Gene Information: Click here.

f. Adjuvant: IL-12 vacince adjuvant

VO ID: VO_0001147

g. Immunization Route

Intraperitoneal injection (i.p.)

h. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: Groups of BALB/c mice 6 to 8 weeks old (Charles River International, Margate, United Kingdom) were immunized i.p. on days 0 and 14 with 25 μg of Hsp60/100 μl of saline with or without 0.5 μg of murine IL-12. Control groups were immunized with either IL-12 only or 100 μl of saline containing eluted protein-free gel. IL-12 was kindly provided by Roche Pharmaceuticals. Blood samples were obtained from each mouse by tail vein bleeding immediately prior to challenge (day 28). The serum was separated by microcentrifugation and stored at −20°C until analysis. Groups of mice were either challenged or culled to provide spleen cells a further 14 days later (Hartley et al., 2004).
Challenge Protocol: F. tularensis LVS, F. tularensis subsp. holarctica HN63, and F. tularensis subsp. tularensis Schu S4 were used for challenges. The strains were harvested into PBS after growth on BCGA for 24 h at 37°C and diluted to a given optical density. Confirmation of the dose was achieved by plating out a serial dilution. Groups of mice were challenged on day 28 with either 103 or 104 MLD of LVS i.p. or 100 MLD of HN63 or 10 MLD of Schu4 subcutaneously as separate challenges. Mice were monitored for 14 days, and survival to a humane end point was recorded (Hartley et al., 2004).
Efficacy: Researchers have shown that mice that had been immunized with purified heat shock protein 60 (Hsp60, groEL) isolated from Francisella tularensis were protected against a subsequent challenge with some strains of the bacterium (Hartley et al., 2004).

4. F. tularensis vaccine LVS LPS

a. Vaccine Ontology ID:

VO_0000308

b. Type:

Subunit vaccine

c. Antigen

Francisella tularensis live vaccine strain (LVS) lipopolysaccharide (LPS) (Dreisbach et al., 2000). The chemical composition of the lipid A moiety of LVS LPS is quite different from that of enteric LPS. The LVS LPS lacks a functional lipid A. This apparently confers a survival advantage on the bacterium, since its LPS does not participate in induction of nitric oxide production that might limit its intracellular growth. Further, since other data indicate that LVS LPS is unable to block macrophage stimulation by functional LPS, the structure must be distinct enough to not permit recognition as an antagonist for traditional LPSs. The ability of LVS LPS to stimulate protection in C3H/HeJ mice, which are defective in the ability to recognize and respond to enteric LPS at least in part due to a point mutation in Toll-like receptor 4, suggests that LVS LPS is recognized through receptors other than Tlr4 (Dreisbach et al., 2000).

d. Preparation

Francisella tularensis live vaccine strain (LVS) LPS is purified from whole F. tularensis LVS bacteria. A culture of F. tularensis LVS is grown in 4-L flasks at 37°C for 48 h in Trypticase soy broth with cysteine. The cultures are centrifuged for 15 min, washed 3 times in PBS, once in methanol, and once in acetone, and then lyophilized. The LPS is then extracted. After the crude LPS is treated with DNase, RNase, and proteinase K, the pellet is harvested by centrifugation 3 times for 12 h. LPS preparations are then reconstituted in endotoxin-free PBS and stored at 4°C (Dreisbach et al., 2000).

e. Virulence

No traditional endotoxin has been associated with virulent F. tularensis. More recent reports indicated that purified LVS LPS was not endotoxic in d-galactosamine-sensitized mice and failed to activate Limulus amoebocyte lysate. LVS LPS also failed to stimulate human monocytes or peripheral blood lymphocytes to proliferate, produce TNF-α, or produce IL-1. Mouse peritoneal exudate macrophages treated with LVS LPS did not produce TNF-α or nitric oxide, and there was no increase in surface immunoglobulin expression by a mouse pre-B-cell line in response to LVS LPS. The only reported biological activity of LVS LPS is activation of complement (Dreisbach et al., 2000). LPS purified from F. tularensis LVS did not activate murine B cells for proliferation or polyclonal immunoglobulin secretion, nor did it activate murine splenocytes for secretion of interleukin-4 (IL-4), IL-6, IL-12, or IFN-gamma. However, in vivo experiments have suggested that LVS LPS contributes to the virulence of Francisella, in that LPS-defective Lpsd C3H/HeJ mice are reported to be more susceptible to LVS infection than Lpsn C3H/HeN (Dreisbach et al., 2000).

f. Mouse Response

Host Strain: BALB/cByJ, C57BL/6J, BALB/c.scid
Vaccination Protocol: Normal mouse serum (NMS) was obtained by bleeding normal BALB/cByJ mice from the lateral tail vein and pooling the resulting serum. Normal BALB/cByJ mice were then immunized with 10 µg of LPS intradermally (i.d.), and immune mouse serum (IMS) was obtained 3 or 30 days later. BALB/cByJ mice were given 0.5 ml of a 1:4 dilution of these sera i.p. 1 day before challenge with 10^3 LVS bacteria i.p. (Dreisbach et al., 2000).
Persistence: T-cell-deficient BALB/c.nu/nu mice treated with LVS LPS survived lethal challenge for 2 to 3 weeks longer than control (PBS-treated) mice; these mice eventually succumbed to challenge after ~3-4 weeks (Dreisbach et al., 2000).
Immune Response: LPS purified from F. tularensis LVS lacks the ability to nonspecifically activate murine B cells for proliferation or polyclonal immunoglobulin secretion. It is also unable to activate murine or human macrophages (Dreisbach et al., 2000). It does not stimulate murine splenocytes to secrete IL-12, IL-6, IL-4, or IFN-γ (Dreisbach et al., 2000). LVS LPS is unable to activate murine or human macrophages. LVS LPS does stimulate specific antibody production and in purified form is a weak immunogen that induces primarily an IgM response. When recognized by the murine immune system as a component of the bacterium, however, the resulting specific antibody response is vigorous and is characterized by the production of large amounts of IgG, particularly IgG2a. Despite the apparent absence of nonspecific immunostimulatory activity and minimal specific antibody production, however, treatment of mice with surprisingly small amounts of LVS LPS stimulates very strong B-cell-dependent protection against lethal LVS challenge within 2 to 3 days (Dreisbach et al., 2000).
Side Effects: None were noted (Dreisbach et al., 2000).
Challenge Protocol: BALB/cByJ mice were given various doses of LVS LPS i.d. on day 0 and challenged 3 days later with lethal doses of LVS, either 10^3 (1,000 LD50) or 10^4 (10,000 LD50) bacteria (Dreisbach et al., 2000).
Efficacy: Very strong protection against lethal LVS infection was demonstrated. Mice given doses as low as 0.1 ng of LVS LPS i.d. survived lethal LVS challenge, depending on the strength of the challenge given. Mice given 100 ng of LVS LPS i.d. survived LVS challenge doses approaching 10^6 bacteria (1,000,000 LD50). Further, in three experiments, 15 of 15 mice challenged with 10^4 LVS bacteria i.p. 3, 10, or 35 days after treatment with 100 ng of LVS LPS i.d. survived; however, in the same experiments none of 15 mice treated with 100 ng of LVS LPS i.d. and challenged 3, 10, or 35 days with 20 LD50 of L. monocytogenes survived. Early protection also could not be demonstrated using other types of LPS, in that mice given 100 ng of E. coli O55 LPS, Salmonella serovar Typhimurium LPS, or E. coli K235 LPS i.d. on day 0 and challenged with 103 LVS bacteria i.p. on day 3 did not survive.

5. F. tularensis vaccine rLm/iglC

a. Vaccine Ontology ID:

VO_0000431

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Antigen

F. tularensis intracellular growth locus, subunit C (iglC)

e. Gene Engineering of IglC1

Type: DNA vaccine construction
Description: Constructed recombinant L. monocytogenes (rLm) vaccines stably expressing seven F. tularensis proteins including IglC (rLm/iglC) (Jia et al., 2009).
Detailed Gene Information: Click here.

f. Gene Engineering of IglC2

Type: Recombinant vector construction
Description: Constructed recombinant L. monocytogenes (rLm) vaccines stably expressing seven F. tularensis proteins including IglC (rLm/iglC) (Jia et al., 2009).
Detailed Gene Information: Click here.

g. Vector:

attenuated L. monocytogenes strain rLmΔactA

h. Immunization Route

Intradermal injection (i.d.)

i. Description

Seven F. tularensis proteins including IglC (rLm/iglC) were expressed in recombinant L. monocytogenes (rLm) vaccines (Jia et al., 2009). These 7 F. tularensis proteins are: AcpA, Bfr, DnaK, GroEL, IglC, Pld and KatG from SchuS4 strain.

j. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: Six groups of 8 mice were sham-immunized or immunized with LVS or rLm vaccines at weeks 0 and 4 (Jia et al., 2009).
Challenge Protocol: At week 8, mice were anesthetized with Ketamine/Xylazine, challenged i.n. with 4400 CFU of LVS, and monitored for survival for up to 3 weeks after challenge. Intranasal challenge with 65× LD50 of LVS. Groups of mice were sham-immunized or immunized i.d. twice at weeks 0 and 4 with 5 × 10^4 CFU LVS or 1 × 10^6 CFU of rLmΔactA (vector), rLm/iglC, rLm/katG or rLm/iglC plus rLm/katG (Jia et al., 2009).
Efficacy: Mice immunized with rLm/iglC were protected against lethal challenge with F. tularensis LVS administered by the intranasal route, a route chosen to mimic airborne infection, and, most importantly, against aerosol challenge with the highly virulent Type A F. tularensis SchuS4 strain (Jia et al., 2009).

6. F. tularensis vaccine X4072(pTUIA-15)

a. Vaccine Ontology ID:

VO_0011548

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Antigen

F. tularensis 17 kDa major membrane protein (TUL4) precursor

e. Gene Engineering of TUL4

Type: Recombinant vector construction
Description: A 1.0-kb EcoRV-SphI DNA fragment was prepared by the technique of Birnboim and Doly (6) from the plasmid pTUL4-9 (39), a derivative of pUC18. The fragment contains the gene encoding the T-cell-reactive 17-kDa protein TUL4 but contains no other expressed open reading frames (40). The isolated 1.0-kb fragment was ligated to the Asd+ plasmid pYA248 (27) after the plasmid had been cleaved with EcoRI and SphI and after the EcoRI site had been made blunt by using the Klenow fragment. The translation initiation codon of pYA248 was followed by a short sequence of 36 putatively encoding nucleotides of the Francisella DNA. The recombinant plasmid, denoted pTULA-15, was transformed (16, 20) into S. typhimunium x3730. By using a lysate of the X3730 recombinant, transduction of phage P22 HT int into X4072 was performed according to standard methods (12, 37). The presence of TUIA in lysates from transduced strains was confirmed by Western blot analysis, and one of the strains, denoted S. typhimurium X4072(pTUL4-15), was selected for the experiments (Sjöstedt et al., 1992).
Detailed Gene Information: Click here.

f. Vector:

S. typhimurium X4072

g. Immunization Route

Intraperitoneal injection (i.p.)

h. Mouse Response

Host Strain: C57BL/10
Vaccination Protocol: Mice were immunized twice, 4 weeks apart, by the intraperitoneal injection of 100 ,ll of PBS containing 5 x 10^3 (5 x 10^2 for the more susceptible mouse strain C57BL/10) organisms of S. typhimurium X4072 or S. typhimunum X4072(pTUIA-15) (Sjöstedt et al., 1992).
Challenge Protocol: Three months after the completion of immunization, mice were intravenously challenged with F. tularensis LVS at a dose of 160 to 230 bacteria. At various intervals thereafter, mice were sacrificed for the enumeration of bacteria in the liver and spleen. The CFU per organ were recorded (Sjöstedt et al., 1992).
Efficacy: A 17-kDa lipoprotein, TUL4, of the facultative intracellular bacterium Francisella tularensis is one of several membrane proteins that induce an in vitro response in T cells from F. tularensis-primed humans. When mice were immunized with S. typhimurium chi 4072(pTUL4-15), some animals showed an antibody response and a T-cell response to TUL4. The present study demonstrated that the 17-kDa lipoprotein TUL4 of F. tularensis is involved in a protective immunity to tularemia (Sjöstedt et al., 1992).

7. Francisella tularensis capB mutant vaccine

a. Vaccine Ontology ID:

VO_0002840

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of capB

Type: Gene mutation
Description: This capB mutant is from Francisella tularensis (Jia et al., 2010).
Detailed Gene Information: Click here.

f. Immunization Route

intranasal immunization

g. Mouse Response

Persistence: A capB mutant is attenuated in mice (Jia et al., 2010).
Efficacy: A capB mutant induces significant protection in mice from challenge with wild type F. tularensis (Jia et al., 2010).
Host Gene Response of IgG
- Gene Response: Following i.n. immunization, LVS ΔcapB induced significantly higher levels of serum IgG, IgG2a, and IgM antibodies in mice than LVS by 7 days post immunization (Jia et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of Ighv1-9
- Gene Response: Following i.n. immunization, LVS ΔcapB induced significantly higher levels of serum IgG, IgG2a, and IgM antibodies in mice than LVS by 7 days post immunization (Jia et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of IgM
- Gene Response: Following i.n. immunization, LVS ΔcapB induced significantly higher levels of serum IgG, IgG2a, and IgM antibodies in mice than LVS by 7 days post immunization (Jia et al., 2010).
- Detailed Gene Information: Click here.

8. Francisella tularensis clpB mutant vaccine

a. Vaccine Ontology ID:

VO_0002841

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of clpB

Type: Gene mutation
Description: This clpB mutant is from Francisella tularensis (Conlan et al., 2010).
Detailed Gene Information: Click here.

f. Immunization Route

Intradermal injection (i.d.)

g. Mouse Response

Persistence: A clpB mutant is attenuated in mice (Conlan et al., 2010).
Efficacy: A clpB mutant induces significant protection in mice from challenge with wild type F. tularensis (Conlan et al., 2010).

9. Francisella tularensis FTL0552 mutant vaccine

a. Vaccine Ontology ID:

VO_0002842

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of FTL0552

Type: Gene mutation
Description: This FTL0552 mutant is from Francisella tularensis (Sammons-Jackson et al., 2008).
Detailed Gene Information: Click here.

f. Immunization Route

intranasal immunization

g. Mouse Response

Persistence: A FTL0552 mutant is attenuated in mice (Sammons-Jackson et al., 2008).
Efficacy: A FTL0552 mutant provided partial protection in mice from challenge with wild type Francisella tularensis SchuS4 (Sammons-Jackson et al., 2008).
Host Gene Response of Ccl2
- Gene Response: LVS induced a significantly higher MCP-1 (Ccl2) response in mice lung homogenates as compared to FTL0552 mice beginning day 3 after infection (Sammons-Jackson et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: LVS induced a significantly higher IFN-gamma response in mice lung homogenates as compared to FTL0552 mice beginning day 5 after infection (Sammons-Jackson et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of IL-6
- Gene Response: LVS induced a significantly higher IL-6 response in mice lung homogenates as compared to FTL0552 mice beginning day 5 after infection (Sammons-Jackson et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of Il12b
- Gene Response: An FTL0552 mutant induced significantly elevated levels of IL-12 in C57BL/6 mice lung homogenates by day 7 post infection compared to LVS (Sammons-Jackson et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of TNF-alpha
- Gene Response: LVS induced a significantly higher TNF-alpha response in mice lung homogenates as compared to FTL0552 mice beginning day 5 after infection (Sammons-Jackson et al., 2008).
- Detailed Gene Information: Click here.

10. Francisella tularensis FTT0918 mutant vaccine

a. Vaccine Ontology ID:

VO_0002843

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of FTT0918

Type: Gene mutation
Description: This FTT0918 mutant is from Francisella tularensis (Twine et al., 2005).
Detailed Gene Information: Click here.

f. Immunization Route

Intradermal injection (i.d.)

g. Mouse Response

Persistence: A FTT0918 mutant showed significantly reduced virulence in mice (Twine et al., 2005).
Efficacy: A FTT0918 provided protection in mice from challenge with wild type F. tularensis 2 months after immunization (Twine et al., 2005).

11. Francisella tularensis galU mutant vaccine

a. Vaccine Ontology ID:

VO_0002844

b. Type:

Live, attenuated vaccine

c. Status:

Licensed

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of galU

Type: Gene mutation
Description: This galU mutant is from Francisella tularensis (Jayakar et al., 2011).
Detailed Gene Information: Click here.

f. Immunization Route

intranasal immunization

g. Mouse Response

Persistence: A galU mutant is attenuated in mice, inducing only transient weight loss (Jayakar et al., 2011).
Efficacy: A galU mutant induces protection in mice from challenge with wild type F. tularensis, inducing only transient weight loss (Jayakar et al., 2011).
Host Gene Response of IL-1b
- Gene Response: Twenty four hours after infection of a mouse macrophage-like cell line (THP-1) or BMDCs with the galU mutant, the amount of IL-1β released into culture supernatants was significantly higher than was observed following infection with WT FT (Jayakar et al., 2011).
- Detailed Gene Information: Click here.

12. Francisella tularensis guaA mutant vaccine

a. Vaccine Ontology ID:

VO_0002845

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of guaA

Type: Gene mutation
Description: This guaA mutant is from Francisella tularensis (Santiago et al., 2009).
Detailed Gene Information: Click here.

f. Immunization Route

Intraperitoneal injection (i.p.)

g. Mouse Response

Persistence: A guaA mutant is attenuated in mice (Santiago et al., 2009).
Efficacy: A guaA mutant induces significant protection in mice from challenge with wild type F. tularensis (Santiago et al., 2009).

13. Francisella tularensis guaB mutant vaccine

a. Vaccine Ontology ID:

VO_0002846

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of guaB

Type: Gene mutation
Description: This guaB mutant is from Francisella tularensis (Santiago et al., 2009).
Detailed Gene Information: Click here.

f. Immunization Route

Intraperitoneal injection (i.p.)

g. Mouse Response

Persistence: A guaB mutant is attenuated in mice (Santiago et al., 2009).
Efficacy: A guaB mutant induces significant protection in mice from challenge with wild type F. tularensis (Santiago et al., 2009).
Host Gene Response of Ccl5
- Gene Response: RANTES (Ccl5) and iNOS were induced after 4 hours of infection of the mutant in mouse macrophages and were significantly higher than media controls. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
- Detailed Gene Information: Click here.
Host Gene Response of Cxcl1
- Gene Response: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC (i.e., Cxcl1), and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
- Detailed Gene Information: Click here.
Host Gene Response of IL-1b
- Gene Response: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC, and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
- Detailed Gene Information: Click here.
Host Gene Response of Il12a
- Gene Response: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC, and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
- Detailed Gene Information: Click here.
Host Gene Response of Il12b
- Gene Response: IL-12 p40 mRNA was induced later in mouse macrophages, but was expressed for a longer period of time than other expressed cytokines. Levels were similar to or greater than levels induced by WT LVS and were significantly greater than media controls (Santiago et al., 2009).
- Detailed Gene Information: Click here.
Host Gene Response of Nos2
- Gene Response: RANTES and iNOS were induced after 4 hours of infection of the mutant in mouse macrophages and were significantly higher than media controls. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
- Detailed Gene Information: Click here.
Host Gene Response of TNF-alpha
- Gene Response: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC, and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
- Detailed Gene Information: Click here.

14. Francisella tularensis IglB mutant vaccine

a. Product Name:

KKF235

b. Vaccine Ontology ID:

VO_0002847

c. Type:

Live, attenuated vaccine

d. Status:

Research

e. Host Species as Laboratory Animal Model:

Mouse

f. Gene Engineering of IglB

Type: Gene mutation
Description: This IgIB mutant is from Francisella tularensis (Yu et al., 2010).
Detailed Gene Information: Click here.

g. Immunization Route

Oral immunization

h. Mouse Response

Persistence: An IglB mutant is attenuated in mice (Yu et al., 2010).
Efficacy: An IglB mutant induces significant protection in mice from challenge with wild type F. tualrensis U112, LVS, and SchuS4 (Yu et al., 2010).

15. Francisella tularensis purF mutant vaccine

a. Vaccine Ontology ID:

VO_0002850

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of purF

Type: Gene mutation
Description: This purF mutant is from Francisella tularensis (Quarry et al., 2007).
Detailed Gene Information: Click here.

f. Immunization Route

Intraperitoneal injection (i.p.)

g. Mouse Response

Persistence: A purF mutant is attenuated in mice (Quarry et al., 2007).
Efficacy: A purF mutant induces significant protection from challenge with wild type F. tularensis (Quarry et al., 2007).

16. Francisella tularensis purMCD mutant vaccine

a. Vaccine Ontology ID:

VO_0002849

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of PurC/PurD

Type: Gene mutation
Description: This purMCD mutant is from Francisella tularensis (Pechous et al., 2006).
Detailed Gene Information: Click here.

f. Gene Engineering of purM

Type: Gene mutation
Description: This purMCD mutant is from Francisella tularensis (Pechous et al., 2006).
Detailed Gene Information: Click here.

g. Immunization Route

Intraperitoneal injection (i.p.)

h. Mouse Response

Persistence: A purMCD mutant is attenuated in mice (Pechous et al., 2006).
Efficacy: A purMCD mutant induces significant protection in mice from challenge with wild type F. tularensis (Pechous et al., 2006).

17. Francisella tularensis sodC mutant vaccine

a. Vaccine Ontology ID:

VO_0002853

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of sodC

Type: Gene mutation
Description: This sodC mutant is from Francisella tularensis (Melillo et al., 2009).
Detailed Gene Information: Click here.

f. Immunization Route

intranasal immunization

g. Mouse Response

Persistence: A sodC mutant is attenuated in mice (Melillo et al., 2009).
Efficacy: A sodC mutant induces protection in mice from challenge with wild type F. tularensis (Melillo et al., 2009).

18. Francisella tularensis wbtA mutant vaccine

a. Vaccine Ontology ID:

VO_0002854

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of wbtA

Type: Gene mutation
Description: This wbtA mutant is from Francisella tularensis (Sebastian et al., 2007).
Detailed Gene Information: Click here.

f. Immunization Route

Intradermal injection (i.d.)

g. Mouse Response

Persistence: A wbtA mutant is attenuated in mice (Sebastian et al., 2007).
Efficacy: A wbtA mutant induces significant protection in mice from challenge with wild type F. tularensis (Sebastian et al., 2007).
Host Gene Response of Ighv1-9
- Gene Response: The titer of immunoglobulin G (IgG) antibodies directed toward the surface-exposed protein antigens in anti-LVS wbtA antisera was determined to be 8,000 + 1,849, fourfold higher than the titer for LVS-immunized mice. The IgG2a subclass predominated among the IgG subtypes analyzed (Sebastian et al., 2007).
- Detailed Gene Information: Click here.

19. Francisella tularensis wbtI mutant vaccine

a. Vaccine Ontology ID:

VO_0002855

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of WbtI

Type: Gene mutation
Description: This wbtI mutant is from Francisella tularensis (Li et al., 2007).
Detailed Gene Information: Click here.

f. Immunization Route

Intraperitoneal injection (i.p.)

g. Mouse Response

Persistence: .A wbtI mutant is attenuated in mice (Li et al., 2007)
Efficacy: A wbtI mutant induces protection in mice, especially in higher doses, from challenge with wild type F. tularensis (Li et al., 2007).

20. FSC043

a. Vaccine Ontology ID:

VO_0000339

b. Type:

Live Attenuated

c. Preparation

Strain FSC043 is a spontaneous mutant of the SCHU S4 strain (Twine et al., 2005).

d. Virulence

Attenuated (Twine et al., 2005).

e. Description

FSC043 is a spontaneous mutant of strain SCHU S4 of Francisella tularensis (Twine et al., 2005).

f. Mouse Response

Host Strain: BALB/c
Persistence: To examine the relative virulence of LVS and FSC043, BALB/c mice were challenged i.d. with CFU of one or other strain and then killed on day 4 of infection, and bacterial burdens in the skin, liver, and spleen were determined. By this time, LVS was present at 100-fold-higher levels than FSC043 in the skin and liver and 20-fold-higher levels in the spleen. The BALB/c mice that were intradermally inoculated LVS showed overt signs of infection before recovering, whereas the mice inoculated with FSC043 remained healthy. When inoculated intravenously, FSC043 grew less well than virulent type A or B strains or LVS in the livers, spleens, and lungs of mice (Twine et al., 2005).
Side Effects: Destruction of skin and inflammation was observed at the site of inoculation of FSC043 (Twine et al., 2005).
Challenge Protocol: FSC033 was used as the challenge strain to assess the efficacy of the various live vaccines employed. For aerosol exposure, thawed bacteria were diluted in Mueller-Hinton broth; for intradermal inoculations, stocks of the strains were diluted in sterile saline. Intradermal inocula were injected into a fold of skin in the shaved mid-belly, and the blister that formed was circumscribed with indelible marker (Twine et al., 2005).
Efficacy: All immunized mice survived and were challenged 77 days later intradermally or by aerosol with type A strain FSC033. Immunization of BALB/c mice with LVS leads to excellent protection against intradermal challenge but only weak protection against low-dose aerosol challenge. The FSC043 immunization afforded a similar degree of protection to LVS against intradermal challenge and somewhat better protection against aerosol challenge (Twine et al., 2005).

21. J5dLPS/OMP

a. Vaccine Ontology ID:

VO_0004201

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

Detoxified, O-polysaccharide side chain-deficient, lipopolysaccharide non-covalently complexed with the outer membrane protein of N. meningitidis group B (Gregory et al., 2010).

e. Adjuvant: CpG DNA vaccine adjuvant

VO ID: VO_0001237

f. Immunization Route

intranasal immunization

g. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: Ten, 6- to 8-week-old mice were inoculated i.n. with 1 μg J5dLPS/OMP vaccine at 0, 2, and 4 weeks. Unmethylated cytidine-phosphate-guanosine (CpG) containing oligodinucleotide (ODN 10104; Coley Pharmaceutical Group, Wellesley, MA) was administered i.n. (25 μg/mouse) one hour prior to each vaccination. Control groups received CpG ODN only (Gregory et al., 2010).
Challenge Protocol: Vaccinated mice were challenged i.t. with F. tularensis SchuS4 on days 35 - 42 post-vaccination (Gregory et al., 2010).
Efficacy: Mice vaccinated with J5dLPS/OMP and CpG ODN resisted infection by type A F. tularensis. Sixty-five percent of vaccinated animals survived challenge i.t. with 10 LD50 F. tularensis SchuS4 (Gregory et al., 2010).

22. KKF24

a. Vaccine Ontology ID:

VO_0000307

b. Type:

Live Attenuated

c. Gene Engineering of IglC

Type: Protein
Description:
Detailed Gene Information: Click here.

d. Preparation

KKF24 is a recombinant strain of Francisella novicida U112 with iglC mutation (F. novicida delta-iglC::ermC) (Lauriano et al., 2003). Strains were grown at 37°C in Trypticase soy broth supplemented with 0.1% cysteine (Pammit et al., 2006).

e. Virulence

KKF24 is highly attenuated for virulence in mice and growth within amoebae (Pammit et al., 2006).

f. Description

KKF24 has been identified as a live attenuated vaccine against subsequent intranasal (i.n.) challenge with the wild-type organism of Francisella tularensis (Pammit et al., 2006).

g. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: Mice were first anesthetized with 3% isoflurane using a rodent anesthesia system and then inoculated i.n. with 10^6 CFU of KKF24 in 25 μl of PBS. Mock-vaccinated animals were treated with PBS alone (Pammit et al., 2006).
Persistence: (Pammit et al., 2006)
Side Effects: None were noted (Pammit et al., 2006).
Challenge Protocol: Vaccinated mice exhibited no signs of morbidity and were challenged 30 days later i.n. with escalating inocula of the wild-type F. novicida U112 strain. Animals were monitored daily for morbidity and mortality (Pammit et al., 2006).
Efficacy: Intranasal vaccination with the F. novicida iglC mutant is highly efficacious against i.n. challenge with the wild-type strain (Pammit et al., 2006). Specifically, vaccinated animals challenged with 100 LD50 of U112 had an 82% survival rate, with minimal losses of body weight . When the challenge inoculum was increased to 1000 LD50 of U112, the survival rate decreased to 50%. Increasing the challenge inoculum further to 10,000 LD50 of U112 resulted in 20% survival. There was no survival of any unvaccinated animals at the challenge doses tested, indicating that all three inocula of U112 were lethal doses, as expected (Pammit et al., 2006).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: It was found that all of the IFN-γ−/− vaccinated mice quickly succumbed within 4 to 5 days to the pulmonary challenge with the wild-type strain, whereas 100% of the IFN-γ+/+ mice were completely protected against both challenge doses. Both groups were able to survive vaccination with KKF24 (Pammit et al., 2006).
- Detailed Gene Information: Click here.
Host Gene Response of Ighg1
- Gene Response: Intranasal immunization with 106 CFU KKF24 induced a robust primary antibody response that included the induction of F. novicida-specific total, IgG1, and IgG2a antibodies. No binding was observed with unrelated antigens, levels in immune sera were significantly greater than normal mouse sera, and levels increased post-challenge (Pammit et al., 2006).
- Detailed Gene Information: Click here.
Host Gene Response of Ighv1-9
- Gene Response: Intranasal immunization with 106 CFU KKF24 induced a robust primary antibody response that included the induction of F. novicida-specific total, IgG1, and IgG2a antibodies. No binding was observed with unrelated antigens, levels in immune sera were significantly greater than normal mouse sera, and levels increased post-challenge (Pammit et al., 2006).
- Detailed Gene Information: Click here.
Host Gene Response of Il12a
- Gene Response: There was potent IL-12 secretion from the stimulated cells upon i.n. vaccination with KKF24 as compared to mice inoculated with PBS. Cervical lymph node and spleen cells were stimulated by UV-inactivated KKF24 10 days after vaccination and then tested for cytokines (Pammit et al., 2006).
- Detailed Gene Information: Click here.

23. LVS

a. Vaccine Ontology ID:

VO_0000271

b. Type:

Live Attenuated

c. Preparation

F. tularensis LVS (ATCC 29684; American Type Culture Collection, Rockville, Md.) was cultured on modified Mueller-Hinton agar plates or in modified Mueller-Hinton broth, and then frozen in broth at -70C; 1-ml aliquots were periodically thawed for use. The number of CFU after thawing varied less than 5% over a 6-month period (Elkins et al., 1996).

d. Virulence

LVS is virulent for laboratory mice and causes a fulminant infection with a histopathology quite similar to that of human tularemia (Elkins et al., 1996).

e. Description

Live attenuated strain (LVS) is derived from the multiple passage of a fully virulent strain of F. tularensis subspecies holarctica and has previously been produced in the US as an investigative new drug. Numerous studies have shown the effectiveness of the LVS vaccine in humans and in animal models of the disease, and protection against aerosol challenge with F. tularensis subspecies tularensis has been demonstrated (Isherwood et al., 2005).

f. Mouse Response

Host Strain: nu/nu, scid
Vaccination Protocol: Mice were given 0.5ml i.p. or 0.1 ml i.d. of the indicated dilution of LVS. To deplete mice of circulating cytokines, mice were treated i.p. with 500 mg of anti-IFN-g, anti-TNF-a, or control hamster immunoglobulin G (IgG) 1 h before infection with LVS. To deplete mice of neutrophils, mice were treated i.p. with 250 mg of RB6-8C5 at 3 d and again at 4 h before infection with LVS for depletion of Gr-11 cells (Elkins et al., 1996).
Persistence: T lymphocytes must be available for clearance of bacteria and long-term survival. Populations enriched for either CD4+ or CD8+ T cells can reconstitute long-term survival and clearance of i.d. LVS infection in scid mice, but over time, reconstituted recipients contain all subpopulations (Elkins et al., 1996).
Efficacy: Scid mice survive i.d. infection with doses of LVS ranging from 100 to 106 for about 20 days. All surviving recipients that cleared bacteria contained both CD4+ and CD8+ T cells in their spleens, including those that originally received highly enriched CD4+ or CD8+ T cells (Elkins et al., 1996). IFN-gamma and TNF-alpha are critical cytokines for the initial survival of infection. Mice treated with anti-TNF-a antibodies or knockout mice lacking functional TNF-a receptors are extremely susceptible to Listeria infection. Similarly, GKO mice infected i.v. or by aerosol with Mycobacterium tuberculosis succumb to infection, as do IFN-gamma receptor knockout mice infected with Listeria spp. The experiments using knockout mice unequivocally demonstrate that there is no compensatory activity available during bacterial infection for IFN-gamma and TNF-a, despite the remarkable functional redundancy generally observed in the cytokine network (Elkins et al., 1996).

g. Human Response

Vaccination Protocol: The vaccine was administered by scarification. A single 0.6-mL drop of LVS vaccinwas placed on the volar surface of the left forearm. A bifurcated needle was used to administer 15 pricks through the drop. The skin surface was then cleaned with sterile gauze, and all excess fluid was removed. Volunteers were given no special bandage or covering for the site. There were no restrictions on showering or wetting the vaccination site. Volunteers were enrolled from 2 iterations of LVS vaccination. From the first iteration, 17 volunteers were enrolled; 10 mL of blood and inoculation site samples were obtained from each person. Baseline skin swabs from this group were performed on the volar surface of the left forearm. Post-vaccination swabs were performed directly at the site of inoculation. Four different personnel obtained skin swabs using similar techniques. In the second iteration of vaccination, swabs were obtained from 24 volunteers using the same time points, but no blood samples were obtained (Hepburn et al., 2006).
Persistence: LVS genomic DNA is detectable at the inoculation site up to 2 weeks after inoculation. This suggests that replicating organisms are present at the inoculation site, which poses a theoretical risk of person-to-person transmission. However, this risk of transmission is likely to be very minimal (Hepburn et al., 2006).
Side Effects: One volunteer recorded a temperature of 38.3°C on day 2. One volunteer developed 4×5-cm axillary lymphadenopathy 28 days after vaccination. No other volunteers had fever or lymphadenopathy. All volunteers had a take reaction. No severe erythema or deep ulcer was observed in any volunteer. During this study, there were no incidents of inadvertent inoculation of remote sites or person-to-person transmission (Hepburn et al., 2006).
Efficacy: LVS genomic DNA is almost universally detectable at the inoculation site for the first 2 days after LVS vaccination and is detectable in for 1–2 weeks after vaccination. Live LVS bacteria were detected in culture for the first 2 days after vaccination. While LVS DNA at the inoculation site was detected well past 2 days, this does not confirm the presence of viable bacteria past this time point (Hepburn et al., 2006).
Description: All but one volunteer had samples obtained at each of the 5 time points. The positive controls for the extraction of the samples taken were positive. The results of blood cultures were negative at baseline and at all time points. Additionally, PCR results of whole blood samples were negative using both tul4 and fopA assays at baseline and at all time points. Baseline skin swab samples were negative for LVS F. tularensis by real-time PCR assays. Skin swab samples were uniformly positive for LVS F. tularensis by PCR on day one, 40 of 41 samples were positive on day 2, and several samples were positive 1 and 2 weeks after vaccination. Two volunteers had negative results on day 7 or 8 but positive results on day 14 or 15. All 9 samples from day 35 were negative with both PCR assays (Hepburn et al., 2006).

h. Mouse Response

Immune Response: Reference is here (Rodriguez et al., 2011).
Host Gene Response of Il4 (interleukin 4)
- Gene Response: Mast cells and secreted interleukin-4 (IL-4) effectively control intramacrophage replication of Francisella tularensis Live Vaccine Strain (LVS), and that mice deficient in mast cells or IL-4 receptor (IL-4R(-/-) exhibit greater susceptibility to pulmonary challenge (Rodriguez et al., 2011).
- Detailed Gene Information: Click here.

24. sodB(Ft)

a. Product Name:

LVS sodB mutant

b. Vaccine Ontology ID:

VO_0000263

c. Type:

Live Attenuated

d. Gene Engineering of SodB from F. tularensis SCHU S4

Type: Protein
Description:
Detailed Gene Information: Click here.

e. Preparation

sodBFt is made by amplifying a region of the sodB gene by PCR. The full-length sodB gene is also amplified. These amplified fragments are cloned separately into a PCR cloning vector and excised by digestion with restriction enzymes. The digested fragments are ligated simultaneously to an XbaI/SalI-digested pPV shuttle vector to yield pPV-sodB. The PstI site upstream of the start codon is used as a marker for screening of mutant colonies. pPV-sodB is then transformed in Escherichia coli S17-1 and transferred into F. tularensis LVS via conjugation. The mutants are selected on modified chocolate agar plates supplemented with Isovitalex, L-cysteine hydrochloride, 1% hemoglobin, 2 µg/ml chloramphenicol, and 100 µg/ml polymyxin B. Colonies obtained on the chloramphenicol plates following the first recombination event are analyzed by PCR, followed by the digestion of PCR products with PstI enzyme. The positive mutant colonies are selected for a second recombination event by plating on medium containing 5% sucrose. Chloramphenicol sensitivity and sucrose resistance following the second recombination event are confirmed by PCR and PstI digestion of the amplified products. The positive mutant clone is referred to as sodBFt (Bakshi et al., 2006).

f. Virulence

sodB(Ft) has increased sensitivity to paraquat and hydrogen peroxide (H₂O2), the redox cycling compounds, as well as with reduced virulence in both C57BL/6 and BALB/c mice (Bakshi et al., 2006).

g. Description

sodBFt is a live vaccine strain mutant of Francisella tularensis with reduced Fe-superoxide dismutase gene expression (Bakshi et al., 2006).

h. Mouse Response

Host Strain: C57BL/6, BALB/c
Vaccination Protocol: Mice were immunized i.n. with 5 × 10^3 CFU of LVS or sodBFt in a volume of 20 μl PBS (10 μl/nare). Unvaccinated mice, which served as a control, received an equal volume of PBS (Bakshi et al., 2008).
Persistence: (Bakshi et al., 2006)
Side Effects: None were noted (Bakshi et al., 2006).
Challenge Protocol: Mice immunized with 5 × 10^3 CFU of either LVS or sodBFt were challenged i.n. with 1 × 10^1 CFU (10LD100) of SchuS4 on day 21 post-immunization (Bakshi et al., 2008).
Efficacy: sodBFt vaccinated C57BL/6 mice not only had a significantly extended MTD (median time to death) as compared to LVS vaccinated or unvaccinated mice, but, 40% (4/10) of the sodBFt vaccinated mice survived the challenge. All naïve C57BL/6 mice challenged with a similar dose of SchuS4 strain succumbed to infection within 6–8 days post-challenge (Bakshi et al., 2008).

V. References

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