VIOLIN: Vaccine Investigation and Online Information Network

Pathogen Page

Mycobacterium avium

Table of Contents

General Information
1. NCBI Taxonomy ID
2. Introduction
Vaccine Related Pathogen Genes
1. relA (Virmugen)
Vaccine Related Host Genes
1. FOXP3
2. IFNG
3. IL10
4. IL17A
5. IL22
6. TGFB1
Vaccine Information
1. M. avium DNA vaccine DNA-Av35/p2AIL12
2. Mycobacterium avium relA mutant vaccine
References

I. General Information

1. NCBI Taxonomy ID:

1764

2. Introduction

Mycobacterium avium is sometimes found as part of a larger complex called the Mycobacterium avium complex (MAC). MAC is an opportunistic infection that takes advantage of a weakened immune system. It can infect one part of your body, such as your lungs, bones, or intestines. This is called localized infection. It can spread and cause disease throughout your body. This is called disseminated infection. With HIV, MAC infection usually happens only after you receive a diagnosis of AIDS (acquired immunodeficiency syndrome) and when CD4 cell counts drop below 50 (WebMD - Mycobacterium avium complex).

II. Vaccine Related Pathogen Genes

1. relA

Gene Name: relA
Sequence Strain (Species/Organism): Mycobacterium avium subsp. paratuberculosis K-10
NCBI Gene ID: 2717869
NCBI Protein GI: 41407145
Locus Tag: MAP1047
Genbank Accession: AE016958
Protein Accession: NP_959981
Taxonomy ID: 262316
Gene Starting Position: 1095829
Gene Ending Position: 1098195
Gene Strand (Orientation): +
Protein Name: RelA
Protein pI: 7.63
Protein Weight: 79917.73
Protein Length: 788

DNA Sequence: Show Sequence

>gi|41406098:1095829-1098195 Mycobacterium avium subsp. paratuberculosis K-10, complete genome
CGTGGCGGAGGAAAACAGCGCGGCGCAAGCGCTTGACGCGCCGGCGGAGTCGCCGCCCAACCCGGTGATC
GAGACTCCCGAGCCGCCGACCGAATCGCTCAAGACGTCCAGCAGCGCGTCGCGCCGGGTCCGGGCCCGGC
TGGCCCGGCGGATGACCGCCCAGCGCAGCACGCTCAACCCGGTGCTGGAGCCGCTGGTGGCGATGCACCG
CGAGATCTACCCCAAGGCCAACGTGCAGCTGCTGCAGCGCGCATTCGAGGTCGCCGACCAGCGGCACGCC
AGCCAGTTGCGCCACTCCGGCGACCCCTACATCACCCATCCGCTGGCCGTCGCCACCATCCTGGCCGAAT
TGGGCATGGACACCACCACTTTGGTGGCCGCGCTGTTGCACGACACCGTCGAGGACACCGGCTACACCCT
GGCCCAGCTGTCCGAGGAATTCGGCGAAGAGGTCGGTCATCTGGTCGACGGGGTGACCAAGCTGGACCGG
GTGGTGCTGGGCAGCGCCGCCGAGGGCGAGACCATCCGCAAGATGATCACCGCGATGGCCCGCGACCCGC
GGGTGCTGGTGATCAAGGTCGCCGACCGGCTGCACAACATGCGCACCATGCGGTTCCTGCCGCCGGAGAA
GCAGGCCCGCAAGGCCCGCGAGACGCTGGAAGTCATTGCGCCCCTCGCCCATCGGCTGGGCATGGCCAGC
GTCAAGTGGGAGTTGGAGGACCTGTCGTTCGCCATCCTGCATCCCAAGAAGTACGACGAGATCGTCCGGC
TGGTCGCCGGCCGGGCCCCGTCGCGGGACACCTACCTGGCCAAGGTGCGGGCCGAGATCATCAACACGCT
GAACGCGTCGAAGATCAAGGCGACGGTGGAGGGCCGGCCCAAGCACTACTGGTCGATCTACCAGAAGATG
ATCGTCAAGGGCCGCGACTTCGACGACATCCACGACCTGGTCGGCATCCGCATCCTGTGCGACGAGATCC
GGGACTGCTATGCCGCTGTGGGCGTGGTGCATTCGCTGTGGCAGCCGATGGCCGGGCGGTTCAAGGACTA
CATCGCCCAGCCGCGCTACGGCGTCTACCAGTCGCTGCACACCACCGTCGTCGGGCCGGAGGGCAAGCCG
CTGGAGGTGCAGATCCGCACCCGCGACATGCACCGCACCGCCGAGTACGGCATCGCCGCGCACTGGCGCT
ACAAGGAAGCCAAGGGCCGCAACGGCGTTCCGCACCCGCACGCCGCCGCCGAGATCGACGACATGGCCTG
GATGCGCCAGCTGCTCGACTGGCAGCGGGAAGCCGCCGACCCGGGCGAATTCCTCGAGTCGCTGCGCTAT
GACCTTGCGGTACAAGAGATCTTCGTGTTCACCCCCAAGGGCGACGTGATCACCCTGCCCACCGGGTCGA
CGCCGATCGACTTCGCCTATGCCGTGCACACCGAGGTCGGGCACCGCTGCATCGGCGCCCGGGTCAACGG
CCGGCTGGTGGCGCTGGAACGCAAGCTGGAAAACGGGGAAGTCGTCGAGGTTTTCACCTCCAAGGCGGCC
AACGCGGGCCCGTCGCGGGACTGGCAGCAGTTCGTGGTGTCGCCGCGGGCCAAGGCCAAGATCCGGCAGT
GGTTCGCCAAGGAGCGGCGCGAGGAGGCCCTGGAGGCCGGCAAGGACGCGATGGCCCGCGAGGTGCGCCG
CGGCGGACTTCCGTTGCAGCGCTTGGTCAATGCCGAATCCATGAGCGCGGTGGCCCGCGAACTGCACTAC
GCCGACGTGTCGGCCCTCTACACCGCGATCGGTGAGGGGCATGTGTCGGCCCGGCACGTGGTGCAGCGGC
TGCTGGCCGAGCTCGGCGGCATCGACCAGACCGAGGAGGACCTCGCCGAGCGGTCCACCCCGACCACCAT
GCTGCGCCGCCCGCGCAGCAGCGACGACGTCGGCGTGTCGGTGCCCGGCGCCCCGGGCGTGCTGACCAAG
CTGGCCAAATGCTGCACGCCGGTGCCTGGGGACCAGATCATGGGATTCGTCACCCGCGGCGGCGGGGTGA
GCGTGCACCGCACCGACTGCACCAACGCCGCCTCGCTGCAGCAGCAGTCCGAGCGCATCATCGAGGTGCA
CTGGGCGCCGTCGCCGTCGTCGGTGTTCCTGGTGGCCATTCAGGTCGAGGCGCTCGACCGGCACCGGCTG
TTGTCCGACGTCACCCGGGTGCTGGCCGACGAGAAGGTCAACATCCTGTCCGCGTCGGTCACCACCTCCG
GTGACCGGGTTGCCATCAGCCGCTTCACCTTTGAGATGGGCGACCCCAAGCACCTCGGCCACCTGCTCAA
CGTGGTGCGCAACGTCGAAGGCGTCTACGACGTCTACCGGGTGACCTCCGCCGCCTG

Protein Sequence: Show Sequence

>gi|41407145|ref|NP_959981.1| RelA [Mycobacterium avium subsp. paratuberculosis K-10]
MAEENSAAQALDAPAESPPNPVIETPEPPTESLKTSSSASRRVRARLARRMTAQRSTLNPVLEPLVAMHR
EIYPKANVQLLQRAFEVADQRHASQLRHSGDPYITHPLAVATILAELGMDTTTLVAALLHDTVEDTGYTL
AQLSEEFGEEVGHLVDGVTKLDRVVLGSAAEGETIRKMITAMARDPRVLVIKVADRLHNMRTMRFLPPEK
QARKARETLEVIAPLAHRLGMASVKWELEDLSFAILHPKKYDEIVRLVAGRAPSRDTYLAKVRAEIINTL
NASKIKATVEGRPKHYWSIYQKMIVKGRDFDDIHDLVGIRILCDEIRDCYAAVGVVHSLWQPMAGRFKDY
IAQPRYGVYQSLHTTVVGPEGKPLEVQIRTRDMHRTAEYGIAAHWRYKEAKGRNGVPHPHAAAEIDDMAW
MRQLLDWQREAADPGEFLESLRYDLAVQEIFVFTPKGDVITLPTGSTPIDFAYAVHTEVGHRCIGARVNG
RLVALERKLENGEVVEVFTSKAANAGPSRDWQQFVVSPRAKAKIRQWFAKERREEALEAGKDAMAREVRR
GGLPLQRLVNAESMSAVARELHYADVSALYTAIGEGHVSARHVVQRLLAELGGIDQTEEDLAERSTPTTM
LRRPRSSDDVGVSVPGAPGVLTKLAKCCTPVPGDQIMGFVTRGGGVSVHRTDCTNAASLQQQSERIIEVH
WAPSPSSVFLVAIQVEALDRHRLLSDVTRVLADEKVNILSASVTTSGDRVAISRFTFEMGDPKHLGHLLN
VVRNVEGVYDVYRVTSAA

Molecule Role: Virmugen
Molecule Role Annotation: A M. avium relA mutant is attenuated in calves and goats. This mutant also elicited an immune response in goats that limited colonization by challenge wild type M. avium (Park et al., 2011).
Related Vaccine(s): Mycobacterium avium relA mutant vaccine

III. Vaccine Related Host Genes

1. FOXP3

Gene Name: FOXP3
Sequence Strain (Species/Organism): Bos taurus
NCBI Gene ID: 506053
NCBI Protein GI: 114052154
Locus Tag: BOS_25920
Genbank Accession: GJ063427
Protein Accession: NP_001039398
Taxonomy ID: 9913
Chromosome No: X
Gene Starting Position: 92369103
Gene Ending Position: 92375460
Gene Strand (Orientation): -
Protein Name: forkhead box P3
Protein pI: 10.15
Protein Weight: 45036.21
Protein Length: 431

DNA Sequence: Show Sequence

>gi|258513337:92369103-92375460 Bos taurus breed Hereford chromosome X, Bos_taurus_UMD_3.1, whole genome shotgun sequence
CACCTTACTTCTTGGTCCCTGCGGGCATGTCCAGGGCTGGTCACCCCTGCCCCCAGCCACTTCGATCCCC
TCCCCTGTCCTCCCTTCTCTTCTTGGCTCTGAGATCAGGGGCCAGGTGTGGGGTTGGAACACCTGCTGGG
CCTCTGGCTCCTCTTCTTGCGGAACTCAAACTCATCCACGGTCCACACAGCCCCCTTCTCGCTCTCCACG
CGTACGAAGCACTTGTGCAGGCTCAGGTTGTGGCGGATGGCATTCTGTGCAAGGAGGACCAGCCAGCCGG
GCCAGGGCCAGGGGGTGGGGGGATCAGGCAGCCATGCACTGATGCCAGCAGTGCCCACCCCAAACTGACC
TCCTGCCCTTTCCCAACACGCCTCCAAGCCCCCTCCCAGGCCCACCTGTGGTGGCATTTGAGGCCCTCCC
AGCCACTGCCCCGTCCAAAGAAGCTCACCTTCCAGGTGGCTGGGTGGTTTCTGAAGAAGGCAAACATGCG
TGTAAACCAGTGATAGATCTCGTTGAGTGTCCGCTGCTTCTCAGGAGCCTCCAGGATGGCCTGGAGGTTA
GGGAGTGTGGGGGTGAGGGGAGCTGCTCCCCCAAATGCAGCCTGACAGGGGCACTGGCCCACCTTGGGCT
GGCCTCTGACCTGGGTGGAATTGTGGGGACAGGGGAAGGGAGGGAGATAGTGAGGGGTGACGGGTCAGAG
ACAGGGTTAGAAGTGGGGTGAGGGTGGGGCTGGGATGGGGTTAGGTGAAGGATCAGGGCTGAGGTTGGAG
TTGCTTCTGGTTCAGGGTCAGGGCTGGAGTGAAGTTAAATTTCTGGGGTGGAGTTGGAGCTCTTGGGATG
GGGGTAGGGTGAGATTTCAGGGTTTAGGAAGTTTAAGGGCCAGGGGTGGGGTACAGTTAGGGTTTGGAAG
GAGTAAAGGGCCCAGCAGAGTTAGAGGTCAGATAAGTGACTGGTTAAGGGTCAGGCTGGGGATGAGGTGG
GGTGAGTCAAGGGGTGAGGGGTGGGATGACGTGTAGCATTAGGTCAGGAGGCAGAGATGGGCCTAGTTAT
TTTGTGTGTCTGGACTGTAGTTAGGTCTTGACTGTGTGTGGTCATTTAAGGATCAGGGATGGGATTGGGT
GTGGAGTGAGGCTGAGGGTCTGGGAATTTGATCTGCTGGGATTTAGGAATGGAATTGCGTCAGGGGTCGG
TGATCATGGCTGCAGTGAGCCTCCGGGTTTGGATGGGGTGAGGCAGGTGAATGAGTGGTGTTGTGTAGGG
TTAGGTGTGGGCCTGGGATGAAGGTCCATGTGGGATCTGGTGAAGCTTTGGGGAAGGCATTAGGGAGATT
AAGATGAAGGGGTTGGGATGGGGTGAGGTGGGAAGGGGTTAGGGGTGGGGTCTAGCAGGGGGATGTCTTG
ACAGACTAGGGTACGTCAGGGCTGAGGAAGGAGTTAGGGTGAACCTGAGGTGGGCATAGGGGAAGGGGCA
GAGATCAGATTAGTTTGGGGGCTATTTATAGGCCCAGGAGGCAGTTAGGCATGGGGTTGAGGTGGTCGTT
AGGAAAGGGGTGGATTTAGGGCCGGCTCTTGGCTTGCCATGGCTTGGACAGCAGTTGAGGTGTGGGTTTG
GGGTGTAGATGGGGAATCACATCTAAGGCATGGGTTTGTTCACACAGGAGGCCCCCTGAGGGTGTTTGGT
GAGGGGAAGGTCCTCCACCCCAGCCCTCTCTTCCTTCTCCTCAGCCTGGCACCCCGCTTACCCAGCGGAT
GAGGGTGGCATAGGTGAAAGGGGGCCGCATGTTGTGGAACTTGAAGTAGTCCATGTTGTGGAAGAACTCT
GCAAGAGGTGGGGAGGGATCAGGGGCCATCTGGGAACTCCTCACTCCCTCTTCCACCCTCTGCTCTCCAC
CAGCTGTCGAGTCTGCATGCCTGAAATCCCACCGTGTACACCGCTTTCCAGGATCACAATCACTGTTGGC
TAAGCACTCTACGTAAATGATACCATTTCCTCTTTACACTGCTCCCACCACTATAGGGCTTATTCTTCCC
AAGGTTTAAAAGTAGTAACTTCCACACCAAAGGGATGTAACTGGTTAAGTGGCGGGGCCAGGAGGGCACT
CAAAGGCCCTGATCCCTGTTAAACCCATAGCCCAGACCTTGTGGATGGCAGAGGATTGGGGAGCTCAGCA
AGTCACCCTGCTTTTTCTTGGTGGTGGGGAGACAAAGAGTCCCTGGGAATTATTAAGCTAGCTCTCTCCC
TCCTGATGACCAAATAGAGTGAGTGGCTGAAGCTCAGAGTGAGTGAGTCCCAACCTCCAGGGAAGACAGG
GCTAGACATCTGAGACCACAATGGGGGCTTGGAGGCCATGGAATTCCAGAGAGGAAGCTTTTGCAAGCAG
TTGCCATTTCCCAAAGGCTGGGTGGCAGTGGCAGGGGAGAGTGGTCCCCAACCTTGTACCAGTTAGGGAG
ATGAGGGGTCAGGGGGCACCGTGTAGGACATGCACCACCCTTACCTGGGAATGTGCTGTTTCCATGGCTG
CCCCAGAGGTGCCTCCGCACAGCAAACAGGCCATCGGGGGCCTCCTGGGGTCCTGGCCAGGCTGGGACGG
TGGTGCCTGGGGTGCCAGTGGCTACGATACAGCAGGAGCCCTTGTCAGATGATGCCTGGGGGAAGAGGGA
GGGGCTGGTGACCCAGAGGCTTAAACTTTCCACTTTAGTTTTAAATAAATGTTTTTATTAACAAAAGCAA
GGGCGTCCTCAATTCCACTCTGATGTGGAATCCGACGAAGCAGCACAAACCATATTTATTAGAGTGGAGG
TGTGGACAAATGGGTGTAGAGCACCAGTGATGGGGCCACAAGCTAGGGTTGAATTGGAATTGGGCTCTTC
TGCCACCCAACTGGGACCTTGAGGAAGTCACCTAAGCCTCCCTGGCCTCAATTTTCCTCAACTGTGAAGT
AGAGATGATAGTAATACCTCACACAGGCATCATGAGGAATTAAATAAGGTAACATCACCAAAGTGCTCAG
AAACAATGCCTGACACCCAGGGCTGGCTATATGATCACTGCAGGTGCCCAGGGCCCGGCACTTTGTTTAA
TGTTCTGCAATCATCACTTTGAAATCCGAGCCCTGTGATTTCATTTTGCACCAGGCCCTGTGAGTTATAT
AGGTGGTTCTGCCAGCAGCACGTGGTCTGTGTTCAGTGTTGGGTGATTTGCTGTTGCTTTGCAAACTCAA
GATCGTGATGTCTGGGGATCTTAAGATCAGAGAGTTTGTAAGCAGAAAGTGACAGATTCCCAGGTCTGGA
TGTGTTTGGAGCTGGGGACAGGGGCCCAGAGATCCCAGGACCTGGGTGCAAGGATAGGTCCCTTGCACCC
TGCAGGAGCCTCTCCCACTGCCCAGCCGTCCGTGCCACCTGTGGGGTCTGGGGTGGCTCACCGCAGATGG
AGCCTTGGTTTGGGCCATCTTCCCGGCCAGATGGGCCTGCATAGCACCCAGCTTCTCCTTCTCCAGCACC
AGCTGTGAGGACAGGCACAGAGTGAGGCCTCGACCCACTCTTTCTCTTCCACTTATTTGCCCGGGCTGTG
GTCTTCCCTGGGAGTGCCCTGCCCCCTTCCTTCCTTCAGGCCAGGCCCCTTCCTGGGTGAGCTTGGCTCC
TGGCATGGGGTGGACTGACCATTTACCACACCCCCACATCACCTGTTGCTCCAGAGATTGCACCACCTCC
CTCTGGAGCAGACACTGCGCCCTGCCCTTCTCATCCAGGAGATGGTCTGCCTGGCAGTGCCTGGGTAGGG
GGAAGAGTCCATGCAGGTGACCATGGTGGGACAAGCCTTCTGGCTCAACATTCTGAGGGGAAAGGGCTCA
GACTCTCTGGGAGGAAGGTCTCTTCAGAGTCTCAGCCTCCCTCCCCTCTTTTGACCTCAGTCTCCCTCTC
ACACCTGTCATTCCTTCAATGCATGCCACTTGGCATCTACTCTATGGCCAGGGTGCTGGAGATGATGATG
GATGGGTAGTGAGCTGAGAGCGGGCCAGGTAAGTGAGAGTGGGAAAGGCATTCCAGATGGAGAGGACCGC
CTGTCCAAAGGCCTGGTTGTGAAGACAGGATGCACTGAGTTGCCACTGATAGTTGGGTATGGTTGGAGAA
GGGGGTGGGTGTGAGACAGAGAGAGAGAAACAGAGTGTGTATGTGTGTGTGTGTGTGATGGGGCTGAGAG
GAGGCAGCAACAGCCACTGTGAGGATCTGGGACTTCAGGGATCAATAAAATCAGGCTGGTGTGTTGGCAT
GACAGGGTAGCAGGGCAAAGCAGTTAGGAGTGTGGATTCTGGAGGCTGGCTATCACTCAGAAGCTGGGTG
ATCTTGATCAAGACACAACCTCGCTGGCCTCAATTTCTTCATCTGTAAAATGGAGGCAATCAGCACACAC
CTCTATTGTGATGCCCCATTATACAGATGAGGAAACTGAGGCTCAGAGAAACTAAGTAATTTGCCCAAGA
TCAGAGCCCATGAGTGATGGAGTCAGGCTCTGAACCCACAGTCTCAGCTTTTGCCTCTTTCATCTTACTC
CCTATCAGGATGAGGCGTGAGCTGAAACCTGCACCCCCAGACCTCCCACAAACTGACCCCCACCCCTGGA
CCAGAATGGGCCTGGTGGGCCCAGCTCACTCACTTGAGGAAGTCTTCTGGCTCCTTGAAGACCTTCTCAC
ATCCGGGCCACTTGCAGACGCCATTTGCTAGCAGTGAGTAGGAGCCCTGGGGCACAGTCGAAAGGGTGCT
AGGGGGGACAAAGAGTGTCAGGGGCGGGTGTGGCAGGGCAGGGAGCCTTCCCAGCCCTGTGCACTGACCT
GTCTTTCCTAGGCATGCCGGGGCTTGGGAAGGTGCAGAGCAGTGCTGGCTCCCTGGAAACCCACTCCAGG
CTGGCCACGTTGATTCCTGCAGGGGGGACAGGGCACCTCTGGGTGCCAGGCTCTGAAGCTTGGCCCCGAC
CAGGCCTCTCGTTTTGAGCTTCCGACCCCCCCCTGAGTTTAGCAGGCCCAGACTCCCAGGGGGCTCTGTG
GTCCCTAACCTCCAAAGCTTCTGTAGAACAGCTCAAAGACTGTGCTGACATTTCGACTAACTGCAAAAGA
TCTGTGGTTCTGGGATTTTGACATTCTGCGTCTTTAAGATTCTGACATTTTTGAGAGGTTGGAGTTTCCA
AGATGATAAGACCTGACCCCTCTGGCCCCCCGAGGGCCGTGATTCAGCAGCCCACACTGCTTTAGTTCAA
GGATTGGGGGCTCTGGGGTTCTGTGGAACTGTGGGGTACAGTGTTACCAGGTGGCAGGCCGGGCCGGGCC
TTGAGAGAGAAGAGCCCCGTAGCAGCAGTGGGTGGCGGGAGGCTGATCATAGCTGGGCTGTCCAGTGGGC
GCACCTGCAGCACAGGGGTCCGGGCATGGGCGTCCACCGTTGAGAGCTGGGGGCACATGAGGGCTGTGGT
TCAACCTGGTCCCCTCCCCAGTTCTCCCGCCTGCTCCACCCTCCCTACCCGTGCTGAGCTGGTACCTGGT
GCACGAAGTGTGGCCTGTCCTGGAGGAGCGCCTGCAAGTGGGGCGAGGGACCCAGCCGAGCTCCGGAGGG
TGCCACCATGACAAGGGGTACTGTGGGCATCTGGGGGAAGGCAGGTGGGTGACATGCTATCCTTATGCTC
ATTCTACATGTCTAATCCCTATACTCCTCACTACAACCCTGCCTACTGGATACTATGACCATCTCCATTT
TACATATGTGGAAACTGAGGCTAAGGGAGACTGGGTGACTGTTTCAAGGTCAAGCCAGCTAGTAAGTTTC
GGAGCTGGCTTCCATCCCGTCACCTTACCTCTGTGGGCCCCCAGCCCTTTGTCAAATGGGGGTGGAGACA
CACACGGCTATGCCTATGGGACGATGATGGGGTCTCCTCTCCTGAGATATGGGTTGGGAGGTTGGTGAGA
GCCTCGAATCTCTGAGACCTGCTATGTGGGGACTTTCTTGGCCCTGTGACATCTGCATAAGTCACAGACT
TGCCTGGGACCCAGAAAACCACTTCCTGTGCCCCAGCTGGGCCCCCCCTCCCAGTGCCACAGTAAAGGTC
GGCACCTGTAGGCCCAGGTGCCCCACCCTGCCTGCCCTATCCTGGACCCTAGGCCTTACCTGCAGCTGTG
ATGGTGGCATGGGGTTCAAGGAGGAAGAGGAAGTGTGGGCCCCGCTTCGGAGATCCCGGCCTTGGAAAGT
TGTCCCTGGGCTCTTGGTGCCCAGCTGGTCTGAGGCCTTGGGTGCAGCCCTCCAGCTGGGCGAGGCTCCT
GGGGATGGGCTGAGTACCAAGGAAGGGGCCAAGGGCTTGGCTGGCCTGGGGTTGGGCA

Protein Sequence: Show Sequence

>gi|114052154|ref|NP_001039398.1| forkhead box protein P3 [Bos taurus]
MPNPRPAKPLAPSLVLSPSPGASPSWRAAPKASDQLGTKSPGTTFQGRDLRSGAHTSSSSLNPMPPSQLQ
MPTVPLVMVAPSGARLGPSPHLQALLQDRPHFVHQLSTVDAHARTPVLQVRPLDSPAMISLPPPTAATGL
FSLKARPGLPPGINVASLEWVSREPALLCTFPSPGMPRKDSTLSTVPQGSYSLLANGVCKWPGCEKVFKE
PEDFLKHCQADHLLDEKGRAQCLLQREVVQSLEQQLVLEKEKLGAMQAHLAGKMAQTKAPSAASSDKGSC
CIVATGTPGTTVPAWPGPQEAPDGLFAVRRHLWGSHGNSTFPEFFHNMDYFKFHNMRPPFTYATLIRWAI
LEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKNAIRHNLSLHKCFVRVESEKGVVWTVDEFEFRKKRSQR
PSRCSNPTPGP

Molecule Role: Vaximmutor
Related Vaccine(s): Mycobacterium avium relA mutant vaccine

2. IFNG

Gene Name: IFNG
Sequence Strain (Species/Organism): Bos taurus
NCBI Gene ID: 281237
NCBI Protein GI: 31982949
Locus Tag: BOS_5228
Genbank Accession: AF140653
Protein Accession: NP_776511
Taxonomy ID: 9913
Chromosome No: 5
Gene Starting Position: 45830157
Gene Ending Position: 45834980
Gene Strand (Orientation): +
Protein Name: interferon, gamma
Protein pI: 10.24
Protein Weight: 18866.5
Protein Length: 166

DNA Sequence: Show Sequence

>gi|258513362:45830157-45834980 Bos taurus breed Hereford chromosome 5, Bos_taurus_UMD_3.1, whole genome shotgun sequence
AATTAGAAAAGAAAGATCAGCTACCTCCTTGGGACCTGATCATAACACAGGAGCTACCGATTTCAACTAC
TCCGGCCTAACTCTCTCCTAAACAATGAAATATACAAGCTATTTCTTAGCTTTACTGCTCTGTGGGCTTT
TGGGTTTTTCTGGTTCTTATGGCCAGGGCCAATTTTTTAGAGAAATAGAAAACTTAAAGGAGTATTTTGT
AAGTATGATCTTTTAATGAGACTTTCTTGTGATTGAAATGACTTGATGTTTGATGTTGACTTGGAATTCT
ATCTATGATGGGCTCTCATCTCTAGTCTTCAGTCATTTTGAGAAGACTTGCTGTTATTGTGACTGTTAGC
TAGATGTGTTTGTTTGTTTGGTTTTTTTTTTTTTTCTGACTAAACAATCTCTGCTCAGTTTGCTATAGAG
ATTTAGAAGGGATTCATGAATCTTTCAAAAGATGGGCATAATATGTGTATACATCATAGTGTTGATATTT
TGTGATAAGAATCCATTCAACTATGATGTTAAGTAGCAAGCATACTGAAAAGACAGCTGAGTCTTAATTT
TTGATTTCTCATGTTACTATAATGATGACAGTCAGAAGTCAAGATCAGCATTAAAAATGGTCATTTGACA
TAATTGATCAATTAAAGAAGATGAGGTCCTTAAATATTTGGCTTAAAAACAAAAACACTTATAGGTGTTT
TTCTCTAAAAAGTCATTTTAAAATTTTACTATCCCATCTAGATTGTCTGTGAATTACTCTTGGACTCAGT
CACTTGCTGAGATTTTTTTTGTAGGAGTTATAATCTAGTTTATATTTTAAAAAATTTTTTGTCTTGTTTT
AGGAATTTCTTAAAAATTTACTTATGGTTAATTAAAATAGCTTGGGCATTTTAAATATTTTATCATATAT
CCAAATTTAGCTGTTATAATTATAGCTGGAGCTATCTTTTAAAGGCGATATAATACCTTATAGGAGGACA
CAGGTAGAGGTTATGAACCGTATACCATAGGGGGGCAGTATTTTATAGTGAGATGATGTTGTTGATTTTT
TTTTTTTTTTTAACTATACCTTTTTCTTACCATAGTAATTTGGGACAGTTGAAGTATCATCAGTCTTGAA
TGAGTTCATTGTGATGTTATATTAGTAAATAAGAAAGCAAAAATCACATGATAGTCTTTGGCTGTTATCC
AACCAGGTAGCTTTTGTGAATATTTTTTGTTAAAACTAAACAGTGTTGAAAAGAAAATCAGTAAATAGTG
CCAGCATCCAAGTTCAATAAAACTTGAGGTACATTTTTACAGCAATTTATGGACTAATTTTAAGCCAATT
TTTTATTGTGTTGTTTTATTTTCCAGAATGCAAGTAGCCCAGATGTAGCTAAGGGTGGGCCTCTCTTCTC
AGAAATTTTGAAGAATTGGAAAGATGTAAGCTGAACATTTCCATTTGGCTGATTTTCCTGTTGCTTTTTT
TCTGATGGATAAATTCACATCAACCTCTCTTTGTGCTCTTTTCTCCCAAGGAAAGTGACAAAAAAATTAT
TCAGAGCCAAATTGTCTCCTTCTACTTCAAACTCTTTGAAAACCTCAAAGATAACCAGGTCATTCAAAGG
AGCATGGATATCATCAAGCAAGACATGTTTCAGAAGTTCTTGAATGGCAGCTCTGAGAAACTGGAGGACT
TCAAAAAGCTGATTCAAATTCCGGTGAGATGATCTTAATGCTTTCTTTGGTATCATTGCAGAGGCTCTTA
CAAAGCACTTCATTCCCAGAAAATAGAAATTAGATACCAGGTGTAAAGCTCTGAAGTGAACTTGTGGTCA
AAATGCCTCTTTGCTAGTTCTCTTTTGACTTGGGTGACTTTGCCGAGTCAGTTATTGGAGGCACTTAAGT
CTGTGATTTTGGGAAAATACTGGCATTATGCCTATCGCACAAAGATGGGTGAATGGACAAATCAGTGAGG
AGGAAGCAGAGAAGGAGTTGGGGTGATGGGGACAGAGTACTGAAAACCAGTTCTCCCATTGCCCCTTGTC
TGTGTGTTGGAAATTCTCTTGCTTTGAATAGGTTGCATGTCTTAATGGAAAGAGCAGTGGGAGGGAAAGG
GAAGAAGATGTGTGCTCCGCCCAGCTTAGCCACCAAAGAAATGTGTGACTTCAGATGAATCACAGGCCTG
GCTGGGGCTGTTTCCTCATCTTAAAAGGAGCCTGTTGAGTTCACTGTAATTTCTACGGTCTCCTTTGCTC
TAAAATTCTACAATGCCATGGAAAGAAAATGAGAATGAGATAGGAGAAAGGAATAGCCTTTGAGGAGACA
AAGGCCCTCCCACTGTCGAACTCTGGTCAATCACCCAGATTGTATAGCCCAAGGAGAGGTGACTGCGTTT
GTTTTTAGAAAGAAAATAGGTAGAATTGTCCTATCAATGAATTTTTAAAGTTTTTAGAAGCTGAAAGATC
ACTTTGAAGCATAAGGACAAGTCACATTTTCCAAAATTTCACCTAAGGGACAACGGTCTCCAGATTTTTT
TGCGGGGGAGAGTTGGCTTTTGTATAAATAGGCTTGGGTGAGAAGAGGAGGGGTCCCATTATGACTTGTG
CTTTCAGGATTTGTACCAGCTCTTGCTCTAACCTGGTCTGCCTCTCAACAATCTCATATAAGCTCCCAGG
AACTGAAATAAGTGGTTGCTCTTCAAGATTTCTCTAGTCTCCCATATATTAGATGGTATGAGAGTGGCTT
GGAAAAATTATATGTAATGTTTTCCATGTTCAGCAGATCTCCATTGGTTGCAGGCTAGAGAATGAAAAGG
CACTGTTCCTGACCCAGTGCTGCCTCTGGTTGCTGTTCAGTCCTAGAGAAGTCCCTTAGTTTCTTGGTGA
TGCTTAGAAAACTGGAAAGTTATCACTTGTAAACACACAGCTGGTAGAAATTTTTCTACTCTGTTGTAAC
TGGGCCTTGAAAACAATAGGTTGGTCCCATCCTTCTGGAAGCTCCAAGCCTCCTTCTTCACAATGTGCAA
TCTTGAGTGAGTGCTCAACCATTGTCGTCTTTTGCTCAAATGTGAACATATTTAAGGCACGTTTTGCCTT
TTTGTCTTGTAACTTAGAATCTCCTAGTCATTCTCAGAAATGTGGCAAGGTGGCTGCAGTGTTTCAGCTT
TAGAAAGAAATGTAGTGGCAGAGCAAAGATGAAAGCAAATGGTCCTCATTTTATGAAGCATGAATTCACC
TATATTGTCCTACTGGGAAGAGTGAGAAAATAGTTCAATTTGCTGGAGTGGAAGGCAGGGTACCGGGAGA
TTGCTTTCATTTCTAACTTTATTATATAATACCAGTAATTGATTACTACCTTTAAATAAGATCCGTGTTT
ATTTTTAATCCCATCATGGAAACAATGAGACTGACCTAATTTGGAGGAAATTAAAAATAATGGTTTTCCT
CCTTCTAGAACTTGGTGTTTATAAAGTACTGAACCTATACCAATGGTTTTCAAACTTGGGTGGTGCCTAA
ATCACCTGGTGATGTTGGTAACCACACAATGGCCCAGGCCTAGTCCGATTTCACCCATCTGGGTGTCAGA
GTTCTTCATTAGGTCCTCAGGTTACTCTTTTACGCACCAAACATGAGAGCCATTGGCTATAGTAACTCAT
TTGATGCTTACAGCGAAGACTAAGTACTAAGTGATAGCAGTGACGGAGACCCTAGGGATCATCTAAACAC
TCTCCTCATACTTAGTGGAGAAAACAGAGATCTGGAGAGCCTCAGTGACTGGTTTAAAGTCAGATGTATT
GCACACACAGAATTCACACCCACATTTTTCTGTCACTCTACTTCTGGGGTTTTTTCACTATATAATTTTC
AGATCTCTGAAGTGGTAAATTTATGAATATGTGATGTGCTCCATCCTGTTACAGTACAACTATCAATTGA
ATTGTTATAATTTTAGTTTTTATCACTGAAGAAACCAACATTACATATTAAGGTTCATTACTGCTAGTGA
AAATAATTTGTTTTAAAGAATACTTTGATTTTCTTGGGCCTAGACAGCAGAATATCCTAATGACTCATAG
GCCTGAAATTAATTTTGCTGTTTTCTTTCCCAATAGGTGGATGATCTGCAGATCCAGCGCAAAGCCATAA
ATGAACTCATCAAAGTGATGAATGACCTGTCACCAAAATCTAACCTCAGAAAGCGGAAGAGAAGTCAGAA
TCTCTTTCGAGGCCGGAGAGCATCAATGTAATGGTCCTCCTGCCTGCAATATTTGAATTTTTAAATCTAA
ATCTATTTATTAATATTTAATATTTTACATTATTTATATGGGGGATATATATTTAGACATCAAAGTATTT
ATAATAGTAACTTTTATGTCATGAAAATGAGTATCTATTAATATATGTGTTATTTATAATTCCTGTATCC
TGTGACTATTTCACTTGACCCTTTTTTTTCTGAGTAACTAGGCAAGTCTATGGGATTTCAAGGTTTTATC
TCAGGGGCCAACTAGGCAGCTAACCTAAGCAAGAATCTGTGGGTTGTGCACTTATTTCACTTGATGATGT
AATGAATGCTGATAAATGAAATGATGCCATCTAGTCACTACTTATCTGAGACTAGATCTGGATTCTGAGC
CACTACTTTGATGGCATGTCAGACAGCACTTGAATGTGTCAGGCTATATGACTTGTGCCCTGACAAAAAC
ATAGCATCTCATCTCACCTTATACCTGGCGCTTCAGGATATCACTGACAATTGTGATTACACCCAAATGG
AAAGTAATATGTTTGTTTAGTTTATCAATATTTAATATATATGAATAAAGTATAATTTCATAAC

Protein Sequence: Show Sequence

>gi|31982949|ref|NP_776511.1| interferon gamma precursor [Bos taurus]
MKYTSYFLALLLCGLLGFSGSYGQGQFFREIENLKEYFNASSPDVAKGGPLFSEILKNWKDESDKKIIQS
QIVSFYFKLFENLKDNQVIQRSMDIIKQDMFQKFLNGSSEKLEDFKKLIQIPVDDLQIQRKAINELIKVM
NDLSPKSNLRKRKRSQNLFRGRRAST

Molecule Role: Vaximmutor
Related Vaccine(s): Mycobacterium avium relA mutant vaccine

3. IL10

Gene Name: IL10
Sequence Strain (Species/Organism): Bos taurus
NCBI Gene ID: 281246
NCBI Protein GI: 41386772
Locus Tag: BOS_15514
Genbank Accession: GJ062448
Protein Accession: NP_776513
Taxonomy ID: 9913
Chromosome No: 16
Gene Starting Position: 4402473
Gene Ending Position: 4406411
Gene Strand (Orientation): -
Protein Name: interleukin 10
Protein pI: 8.14
Protein Weight: 19616.38
Protein Length: 178

DNA Sequence: Show Sequence

>gi|258513351:4402473-4406411 Bos taurus breed Hereford chromosome 16, Bos_taurus_UMD_3.1, whole genome shotgun sequence
AAATAGCTCACAGAGAAGCTCAGTAAATAAATATATGTGGGAGCTGAGGTATCAGAGGTAATAAATACTC
TAGGGGAGAGGCACAGTACAGGTCTCCAGGGAGCTGGCTCTGCTCTCCCAGAACCCTGGATTGGATTTCA
GAGGTCTTCCGTTTACGTCATGGAGTCTAGTAGAGTCACCATCCTGGAGGTCTTCTTCCCTAGAATGCTT
CACTTTTGCATCTTCGTTGTCATGTAGGTTTCTATGTAGTTGATGAAGATGTCAAACTCACTCATGGCTT
TGTAGACACCCCTCTCTTGGAGCTGTGTGGGGAGACAAGACGTGTTCAGTGAAAACTCCCTCTGAGCAAG
TGAGTGGGTCCCTGCTGGGAAGGTCATGACCCTGGGCCCAGAGGCTGCACTAGCTTGCTTGCATTGGGAG
ATGAAGGACAGACTCTGGGCATCTGGAGAGATGCCGGACTGTCAGCAAAGGCTATGCGTTGCCCTGGACC
TTGCCCGGTGGGCAGCAAGAGGAACATTCACTTTGCAAAGTGTGGTTTTTCGAACGGTGCTTTTCCAGCA
ATTTTAAACGTCATGTGCTCACGTGACGCTTATTCAATATCTGATGAAAACACATTAGCGGAACCACTTT
GAAAGAAAGACAATGAGAGAGAGAGAGAGAGGTAGCCTTGTTCCTGTTTATGTTCCCCCACCCGTGGAGT
GAGCAGGCCCAGCCCCGCTGCTCTGACCTACTGCAGGGAGGTTCATGGCCCCTGATACTGGAACCTGCCC
AGGGTCTGTCCCCCACCCACGAGGAGGACATAGGTTCCGCGGCTTCTGGAAAATGGGGTAGAAGAGCTCA
TGGACAGCTGGAAGTGAGAAAGGCCACTTCGTTTGAGAGCTGAGATTCTCAGGTGCCTTGTGTGGTGAGG
CTGGCGGGTGGAAAGAGAGGGGTCAAGTCAGGTAAGGAGAGCTCTGCTTCCTGCTCTGGGCTCGGGGTGC
TCAGGCTGCGCCCCTGGGTGTGTTTGTAAGTGCACGTTCACGTGTCAGCTTCCTGCACGTGCGGCTTCAG
ACTGGATCCCAACAAGCTACTTTCTGTTTCCTCTGTCACTCTCCCCAGCACCCCGCTCGCTGTGTTCACA
GCTGGCGGGCCAGCCCAACCGGCAGGCCTGCGAAGGCAGGGAGCAGTCATTTAGAAACCCCCAAAGACAC
TTCACAGAAAACAAATATAACTGAGGTGGCGGCCCCTCACTGGATGAAGAGGTCACCCCACTTGCCGGGT
TCCCACACTCCCTCCGAGCAAAGTTCAGGGCCTGCTGAGCACCTGCCCACCTCTGCCCCTTCGAGAGTGG
ATGGAGGTCACGGTCCTCAGGCCCTCCCTGCCAGCAGCCCAGCCTCACCTCACTGAAGACTCTCTTCACC
TTCTCCACCGCCTTGCTCTTGTTTTCGCAGGGCAGAAAGCGATGCTGTGGAAAAGGAGAGAGTATGAGCA
ACATTGGTGGCTGGTCCCAGATGCACCGTGCTTGGGAGACAGCCTGGGAGCAGGAGGAGGCCAGCTCATC
TTGAAGGCCCACCCTGCATCTCAGGGAGGACTGAGCCTGGGCACCCTTCCCCACCAGGCAGAGAGGAAGC
ACAGCAAGGCAGGGGCCAGGCGGCATGTGTGTCTCTAGGAGCTCCTCCCCACCGTTAACAAGGCTGCATC
AACACCTCTGTCTTGGAGTTTCCTTCCGGGCGCATCCCAGTGCCAACCAGACTGCTGAGCCAGCCCTTCT
TCCATCCTGTCCTGCTTCTGGCCTGTCCCCCTCCTTGGTCCCAGCCACAAGGACCATTTCTAAGTGCCCC
GCAGAGCAGAGACACAGGGTCAGCCCTGCCAGCCAACCCCCTTGCCTAGCGCTGTCCTGCAAATACAGCC
CAACTGATTTCTCAACATCGGCGAGGGAGCAGAGGAAACTGGTGGGGAGGGTATCATTGCAGTTGGAGGG
ATCAGTAATCCTGGGGGATTATTCGCAGACACTAGCCTGGGACCAAGGATCCCCCTGGCTCTTCTAAAAT
GCCAGGACAGTCCTGGCAGTCAGCCGGGCCCCACCTGAAGCTCTGCTTTCAAAAGGTCGAGAAGAAGAGG
AAGGGGCCTTCCCTCTCCCAGGCCCTCTGCTTCTGCCTGCTTCCGTGGGGCCCAGGGCCTCTGTCCCCAG
GGACAAGGGAGCCCTGGCTGAAGCTCTGGGCCCCTTTCACGTCGAGGCCGGGGACTGCTAATAACCCACA
AATGACTCACAAATAACAGGAAAGCTATTGCAAATCTTCACATGTAAGCACTCAGCAACCACCCCCACCC
GCGGCTCAGAAACAGCCGAGCCAGGCCTGGGGTTCCGGAGGGGCAGGGGCCCCGAGGGCTGGTGTCCCGG
GGTCTCTGGGCGAGAGCAGAGCTTGAAGAGGAGGTCTCAGCACGAGCCCAGAGGAGCCAGAGCTGGCCTG
CTACTCACACAGCGCCGCAGCCGCAGCCGGAGGGTCTTCAGCTTCTCCCCCAGTGAGTTCACGTGTTCCT
TGATGTCAGGCCCGTGGTTCTCAGCCTGTGGCATCACCTCTTCCAGGTAAAACTGGATCATTTCCGACAA
GGCTTGGCAACCCAGGTAACCCTGAGGGCGGAAACCAAGGCGGGTGGTGACCCGGGAGGAAGGGCTACAC
TGCCTGAGAGGACGGCTCTGTTCCCCAAGGACCTTGTTCAGTGGCCACCTCCCCCTGGAACGGAGCCCTT
GGCAACACCCAACACCCTGCCTCCCTCTGGCTATGTGCTGAGTTAAGATCTTCCCACTTCTCCTTTTTTT
GAAGTGAAGGAAGCAGACCCAGCTCCCTGCAGGCGAAGAAGCAGAGTCTCCCTTGGGCACACCGCAAGCT
CCTTGCCCCCGCCAAACGCACTCACCCCAGCCCTCACCTTAAAGTCATCCAGCAGAGACTGGGTCAACAG
TAAGCTGTGCAGTTGGTCCTTCATTTGCTGCAAGAAGAACAGAAGGAGAATGAACCCAAGGTTTGGAGCA
ATACTGAGAAGGACTGTCTTTCTGATATCCTTTTATTGAGAGACTTTTTGATTTTTAGTCAACACAGCCC
TGTCTCCAAAAGGGAAAGGAGAGGTTTTGAAACAGGACATTCAGCAGCGCTGAAGCCCTTAGTTAAACCA
GGTCCTTCCTCCGGGGATCAAGTTCTTTTAAAAACCTGGCTGATTTTGAAGGCAAGGCAGAAGCCTGCCT
GGGTCCCAGTCTTCTTTCTGTAGAGCTGCGTCACGTGTGCAGGGACCCCCATTCCCAACCCCTCAGCACC
CTCTGCTTTGCTGTCTCCAGCTGCAGCACTGACCGTTCTCTGGGAGTGGCAAGCATCTTCTCCTGCAGGT
CTCCCCCTGTATGCGCTACCTCCAACCCATTTGTCATTCAGGTTCTGGAAGCCCTCCAAGCTGAGGCAAC
TTTTGTATCCCTGCCACGGGGTTTTGGGAACATCTTCACCTCCAATCAGATCTCTCCCAGCCAATGAGGT
ATTGAGCTCCAGAGGAATCCTTTACAGATTTTCTAGCATAGGCTAGATGTGCCTTTACATTTTTAAAGAC
AACTTTTCTTGCACAATCTGCTACATCACCAGAGCTCAGATTAACTCACTGAACCCACAGTTTGACCAGG
GACTCTTAAGAATTTCAGCTTCGAGGGGAATGGGTGCTGAGGGTGGAGGTGAGTGAAAAGGAGGCTCTTG
TGAGTGCCTGCAGAATGGCTCTTCCTGCGGTCCTAAGGGAGAAATGAAGGACAGGAGGGCTTTATACTCA
CAAAGAAAGTCTTCACCTTGCCGAAGGCAGCTCGGAGCTCCCGCAGCATGTGGGGCAGGCTGGTTGGCAA
GTGGATACAGCTGCTGTCAGACAGGGTGCTCGCATCTCGGCTGGCTGCCACCCCAGCCAGGAAGACCAGG
CAACAGAGCAGGGCTGAGC

Protein Sequence: Show Sequence

>gi|41386772|ref|NP_776513.1| interleukin-10 precursor [Bos taurus]
MHSSALLCCLVFLAGVAASRDASTLSDSSCIHLPTSLPHMLRELRAAFGEAKTFFQMKDQLHSLLLTQSL
LDDFKGYLGCQALSEMIQFYLEEVMPQAENHGPDIKEHVNSLGEKLKTLRLRLRRCHRFLPCENKSKAVE
KVKRVFSELQERGVYKAMSEFDIFINYIETYMTTKMQK

Molecule Role: Vaximmutor
Related Vaccine(s): Mycobacterium avium relA mutant vaccine

4. IL17A

Gene Name: IL17A
Sequence Strain (Species/Organism): Bos taurus
NCBI Gene ID: 282863
NCBI Protein GI: 56605852
Locus Tag: BOS_21471
Genbank Accession: AF166487
Protein Accession: NP_001008412
Taxonomy ID: 9913
Chromosome No: 23
Gene Starting Position: 24346987
Gene Ending Position: 24350508
Gene Strand (Orientation): +
Protein Name: interleukin 17A
Protein pI: 9.15
Protein Weight: 15852.85
Protein Length: 153
Protein Note: Also known as IL17; IL-17

DNA Sequence: Show Sequence

>gi|258513344:24346987-24350508 Bos taurus breed Hereford chromosome 23, Bos_taurus_UMD_3.1, whole genome shotgun sequence
TGTCCATCTCACAGCGAGCACAAGTTCATCTGTCTACAGTGAACTGGAAGGAACTACCATGGCTTCTATG
AGAACTTCATCTATGGTGAGTCCTGAACTGATGTGTGATTCTCTTCTCGATTTAGGCCAGATCAATTTCT
GGACTGCGGGCATGAAATTCTGGATTCTAACTATGGAAATACAGTCTATATAGGATGGGAAATGAAACCC
TTGATAGATATTTGTCATTATTGAGCATTTTCAAGAACTGGTGCATATCAGTTTGAAAGATGAGATTAAA
ACTGAAAGATAGCTAGACTGGGGCTAAACCACACAAGAAGTGTCAACGTGACATTGTGCTGTAAGAGAGA
ATGCTTATTGAAAATCTGTTTCTCTGGGTACAAGGGATTTAATATAATTCTTCCACAGTGTTTTTCTTTA
ATTCAAAATAAGATGGGTCACTACCCTTGAAGATTTAATGTTTTGGCAGTGACTCTTCTTTCCATTATTG
TATTATTATACTATAGTCTTGGTACACTAACCCTATTTTCTTAGGTATGTTTCCCAGCTTTTCCAAATTT
ACATATATTGATTCTGTAATCAAATTGGATTTCAAAAAAGATGCTTTAACAATATTTTAAAAACAGAGAC
CTTTTAATTCTAATCTAAAGTTATACATGCGTCTTTTTTGAAACCCAATTTGATTACAGAATCAATATAT
GTAAATTTAGAAAAACTGGGAAATATACTTAAGAAAATATAAGAGGGTGTTGAGTTTGGGCTTGGACATT
TGCCAGTGGTACCAACAGAATTTCTAACTGATTCCACATGGATTTTATACAGCCCTTTATGTCTAAGGTG
TGCATTCCCTTTTATGCAATGTTTCACAAAATGTAAGAGGAGATATCAATGGTGTAGAAGGGCATTTCTT
CCTAGAAAATCAAGGTCATTACCCTTCCAAAGACCTGAGAGTGATGCATATACTGATATATTGATGACTT
GCCATCTTCACTAAAATTACTGTCAGCCAATGAATAATGTGGTTTTTCTACTTTAAAAAAAAATGTTCAA
GACTGGTAGAAATGCCATGGTCCTAATGTCACTTTAGTCATCTCATCAGCTTTTCACTTAACATATTTGA
AAATTTAGTATAGATTGTCCTGGACCATAGAATGTTCTCTACTATCAGCAATTTAATCATTACCACAGTG
TGTTAACAAAAACCATTGTAGTACTAACCCAATCTACCTCTCTCTCCATCCAGTCACTGCTACTGCTTCT
GAGTCTGGTGGCTCTTGTGAAGGCAGGAGTCATCATCCCACAGAGTCCAGGCTGCCCACCTACTGAGGAC
AAGAACTTCCCGCAGCATGTGAGGGTCAACCTAAACATCGTTAACCGGAGCACAAACTCCAGAAGGCCCA
CCGATTATCACAAGCGCTCCACCTCACCTTGGACTCTCCAGTACGTAAGCACCCCAGATAAAACCTCTGC
ATTCTTCTACATCAGAAGAACACATCAGACTGCCAGTTAAATCATCTGAGGGGGGTGATATTACTCATTC
AGAATCACCTGTCAAACCTGGAAGAGCCATTGTTCAAACTAGTGATTCTCCAATTTTCTTTCTACAGATT
TCTTTCATAGATCACAGCTCTCCCCAGCCCATCCATAACACCTAGCCTACGAGTTCTCGATGTCCCCAGA
ATCCACTTAAATGAATGGTGGTCATCATTCTGATATTTATGACACCCCAAGTCTATTGTTTATTTACCCA
AAAAAGAAACAGACATTTAGCATGGGAGATTAGTACTGGAATTCTCTTTCCCAGGGCTACCAGAGAATAC
CCTGGTAGAGTTTGTTGACAACAACAATCAATTAACTGGGATTTTTGTGCTTTTCTTGGTTATATTAGCA
CTGGGAAAAGTGGATTAGCTACTGGATTAGCTTGAATCCCAGTAGAAGAAGTGTTCCATTGGGTGTTTCA
CCGTTTTTTGACTTAGTCTTGAATTACATCCCCAACAGACTGACATTCGCTGTGTCTTTAATACATCTAG
TGGTGGTGGTGGTGGTGGTGGTTTAGTCGCTAAGTCGTGTCCGATTCTTGTGATCCCATGGACTGTAGCC
CACCAGGCTCCCCTTTAGACCCTATGATGGATCAAATACAAAATGCATTTTGATAATTTGATTTCAGGCA
AATGATCTCAGTTATGTGGAGCCTACAGATTCACTTGTCTATAGGGTCTTTTCTTCTTACTTACTTACTT
ACCAAGAATAGTTCAAATAACTTAAACTAAGTCCAATCACTCTCTCAGCCAGAGGAGAGGGAGAAGGGAA
AGTTACAGAGGGGCTTCAGATATTGTTTCCAGCCCTTCCTGTCACCCGTGGGCTCTGCTCCATCAGTGTG
TTGTCAGATAAATTTGGATGCCTTTCTTGTTTCAAAGACAGACTTCATTAAGTCAGATTAGCAGCTATCT
TGTTTCTAGATGACTCTTAGTTTTTTTAATCTATCAAATGCACCCTAAAAAAATATCCTTTATGACAAAC
AAATCCTTTCTGTAGTTTCAGTCTGTCTTCTTTTGTTTATTCTTCAAAGAACCTGGGGAAAGCTGATCAT
TGTTTCTTTTTTCTGTAACATTTCATGGGCTTCCAAATCCAAGTTTTTCCCAAATGGCTTGTTTTCCAGA
CTAATAGCACTTAGAGAATTTGCAATGCCTATCATGTCAATTTGCCTCCCTTTGCTTATTCCTGTTTGAT
TTCCTTACTGCTCTACCAGGAATTCACTTTCTTCCTGGCTTTTGTCTCCCCTGCAGCCGCAATGAGGACC
CTGAGAGGTACCCCTCTGTGATCTGGGAGGCCAAGTGCAGCCACTCAGGCTGTATCAATGCTGAAGGCAA
GGTGGACCATCACATGAACTCTGTCACCATCCAGCAAGAGATCCTGGTCCTCCGAAGGGAGTCTCAGCAC
TGCCCTCACTCCTTCCGGCTGGAGAAGATGCTGGTGGCCGTGGGCTGCACCTGCGTCACCCCCATTGTCC
GCCATTTGGCTTAAGAGCTTTCTGCCTGACCCCTACTCCCCAAATTAGTTAGGTTTCCTGGGGAGTAGGC
CCAGCCCCTTTTCTAACTAACATCATTTATGTATTTAATAAGCCCTGAAATAACTTTGGGGCATAAGATT
CCACTTTGATGTATTACATGCTTTATTTTCTATTCTTTTAAGACAAGTTTCCAGATTTGTGAATATTATT
ATTTAAAAGGTAGAACCTATATTTATATGAGCTATTTATGGATCTATTTATGTTTCATTAGTCTTTAGAA
AAAAGGTGAAATATATGAGTATCTGTTTTGCCTAGGGAAATCCTAGATAGGATTAAATAGCAGTTGAAAA
TGTCTGAGCTTGTACTACATATGGCTATAGAATTTTCTCTTGCTTGTCTCTTTGGAGTACATAACACAGC
TGAAAAGAGACTAAATTTAGTTTCATATTTATTAACTTAATTTTGTAAATTATTGAGGCATTACAAGAGA
GACAGCAAGTCTAACTGCTCTA

Protein Sequence: Show Sequence

>gi|56605852|ref|NP_001008412.1| interleukin-17A precursor [Bos taurus]
MASMRTSSMSLLLLLSLVALVKAGVIIPQSPGCPPTEDKNFPQHVRVNLNIVNRSTNSRRPTDYHKRSTS
PWTLHRNEDPERYPSVIWEAKCSHSGCINAEGKVDHHMNSVTIQQEILVLRRESQHCPHSFRLEKMLVAV
GCTCVTPIVRHLA

Molecule Role: Vaximmutor
Related Vaccine(s): Mycobacterium avium relA mutant vaccine

5. IL22

Gene Name: IL22
Sequence Strain (Species/Organism): Bos taurus
NCBI Gene ID: 507778
NCBI Protein GI: 148356272
Locus Tag: BOS_5234
Genbank Accession: EF560595
Protein Accession: NP_001091849
Taxonomy ID: 9913
Chromosome No: 5
Gene Starting Position: 45721766
Gene Ending Position: 45727534
Gene Strand (Orientation): +
Protein Name: interleukin 22
Protein pI: 9.05
Protein Weight: 20001.55
Protein Length: 190
Protein Note: Also known as IL-22

DNA Sequence: Show Sequence

>gi|258513362:45721766-45727534 Bos taurus breed Hereford chromosome 5, Bos_taurus_UMD_3.1, whole genome shotgun sequence
CACAAGCAGAAGCCTCGATACAGGTAAGTCCTCCAACGAACTTGGTAGGATTGGCTTGCTGGGTCTATTC
CTTCTGGATACTTAATTTTTTCCCTTAATGTTGATTTTGTTTCTCGTTCTCTTGATACCAGGTTCTTCTT
CCCTAGTCACCCATTGCTCAGGTTTGCTTTGTCCACGATGGCTGCCCTGCAGAAATCTGTGGGCTCTCCT
CTCAGGGACACTCTGGCCGCTGGCTGCCTCCTTGTCATGGTGCTGTGCGCCCAGAGAGGAGCGGCTGCAC
CCATCACGTCGCACTGTAGGCTCAACGAGTCCGACTTCCAGGAGCCCTACATCTTCAACCACACCTTCAC
TCTGGCTCAGAAGGTACTCATCTCAGTCTTTCTCTTTTGCATCTTATCTACTTGGAACCTGAATAGTTCT
TAAAATTTCTTCAGAGGACATCTAAGATCTTTAACAGATGAACATTAATCGCTGCTGGTATACCTGTCAT
CATTTTCTATGTCTCTTTTAAGACTTTTTGGAGATCAAGCTTTACCTCAAGCCCTTCCATTTGCCCGTTA
GGAAATGTACATTCAGTTTTACCCCCAAAGGGGCCATGTAGTAACCCATCTGACTGTTTATGGTTCCCTT
TACTTCTCTAAGCATTCTTTTAGTTTTTGAAGAGAAAGAAATTGACATTTTTGAGCACTTACTATGTTAT
GAGCTCTATTCTAGGAGCTTTAAATCCATTCTTTCTTTTTTACTGAAGTATAGTTCATTGACAGTATTAT
TTAAGTTCCAGGTATACAGCATAGTGATCCACAATTTTTAAAGATTATTCTCCATTTATAGTTATTATAA
CATATTGGCTATATTACCTATGCTGTACATTATACTGTTCTATCTTATTTTTTAAATTTATTTTGAAGTA
TAGCTGGTTTACAGGGTCATGTTAGTTTCCAGTGTACAGCACGCTGATTCAGGTCTATATATATATATAA
ATATACGTACATATGTGTATATATAATATATGCATATTCTTTTATATATTATTTCCCCTTATTGGTTATT
ATAAAATATTGAGTATAGTTCCCTGTACTATTTAGTAACTTCTTATTAGTTGTCTATTTTATACATTGTA
GTGTATATATGTTACTCCCAAACTTCTAATTTATCCCTCCCCTCAACCCCTGAGCATCATTTTAAACCCA
TGCACTAAAGAATCCTTTCTTTAGTCTTCACAGTGCTGCTCTGGGAAGAGGGGACAGAGTACAGCTTTGC
TTACAGACATTTCTTCTAGCTTTATGATGTCCAGGCACTTAGTCTTTTCTTTTCTTCTAGGCTAGTTTGG
CAGATAACATCACAGATGTTCGTCTCATTGGGAACAAATTGTTCCACGGAATCCATGTAAGCCATAGGCA
TGATGCCACTTGTGGTTACTTGGAGTGGTGGTGAGGATGGGCTGGGTCTTGTGGATGTCGCCTAATTTGA
GCCCTGCTGTTTCACTTTCACCAGCAGGTGACGAAGCGCTGCTACGTGCTGAAGCAGGTGCTGAACTTTA
TTCTTGAAGAGGTGCTGTTCCCCCAATCTGACAAATTCCACCCCTATATGGAAAAGGTGGTGCCCTTCTT
CTCCAGGCTCAGCAAAAAGCTAAGCCAATGTGTAAGTTCTTCTCCAGCCTGAGTCCAATCCTCCCAGTGC
CTGCCTTTCCTCTTTCTTCTGTGTAGAACCCTCTTCCTTCCCACCCCAGTGCCCCTTCCATGCTGGTAGT
GAGAGGTGCCTCTGTCACACCATTACAGAAATCATTTGGCATCACAACCTGTTTGCTTTTGCTTTAACTG
AGTCACATTTTGAGTTGATTGTGATGGGCAGGGTCTGATAGACCACAGGAGGCTCATTTGTCCCTGGGAA
AAAGCGATTTGGACTCTGTGAGATGAGAAAGGTGTTGGGAGCTGTAAGGAAAAATACCTATTGGTGCAAA
TGGATTCACTGAATATAAGTTGATGGAGGGAGGGGATGGGAAAGAGAGAAAGGGAGAAACAAGATTTAAT
ATTCGGCAGTGGGTGGGTGGATGAAGACTGCTGCCCTTTTGATAATGGAAGTGACAAAATCAGAGGTGCA
TGAGTTTGATGCCTCTGAATATTGAAAACTAAGAAAAATATTTTGGTGGAGTAGGCTGAGCAAAAGGGGC
CTACTATTTATTGAGGAGGGCCCTGTTCACATAAGATGTTTTATGTAGGTTTCTTGTTCTGAGCCCATGT
TTTTGATGAGGTGCCGTTATGGTTTTATTCTTGACAGGATTTGCATAAACCACTCCGCTCTTTCCACAAA
CTTCAACCTCATTAGGGTTGCCCAGAGGTGAAGTGAGGTCCCGTGTAAGGGAAATGACTGGATTTTGGTG
TCCCAGGGAATTGAAGAGGTCAGAAAATCTAGGTCACTATTGATATCTAGGTCATTGTGGGCAAAATTGC
TAAGAACTTTAATTCCAGATGAATTGTAACATACTTCAGTTAGGAAGGCTGAGTTTCAAAATCTTCAGTA
CAACAATGTTAAACTTGTTACTCTCATTTCCATTTTGTAGCATGTTGAGTCTGACAACCAGCACATCCAG
AGAAATGTACAGAATCTGAAGAACACAGTGAAAAAGGTACTACTAACAACTATTAGTACTAAATCATTTG
ATAGAAGAGACAAATATTGTTTTTCTTCCCTTTCTTTCCTTTTTTCTCCCACGTCATGATTTTTTACTTG
ACTCTCCTGCCATTGGGTTGATTATTCAGGTGCACATTAAGTATAGATGTGTCTATGCTATGCTATGCTA
AGTCACTTCAGTCGTGTCCGACTCTGTGTGACCCCCCAGACGGCAGCCCACCAGGCTCCCCCATCCCTGG
GATTCTCCAGGCAAGAACACTGGAGTGGGTTACCATTTCCTTCTCCAATGCATGAAAGTGAAAAGTGAAA
GTGAAGTCGCTCAGTCGAGTCCGACTCTTAGCGACCCCATGGACTGCAGCCTACCAGGCTCCTCCATCCA
TGGGATTTTCCAGGCAAGAGTACTGAAGTGGGGTGCCATTGCCTTCTCCGTAGATGTGTCTACATGTTTA
TAAGTCAGTTTCCAAATTTTGCAAATTCTAGAACTGGCTGGGAACTTAAGACATTGACTTATTGTCACCC
ATGTGCTAGGGATGGCCAGTGTCAGAGTCAGAATAGACTGCAGGACCTCTGAATCCCATCCAGGCCAGAA
GTGCTGGATATTATACTTAGTCTCATTAGTTCTTAGGGAAATTTTAGATACCTACTGATTCATTTAGTCT
GAAGAAGCATTTGTTCAAAACCCAAAACTGGGTAAAACTTATTTGAAAGAATTTTGAAGTCATTCACATG
ATGATGTAAAACTTGAGAAATATACCTAGAACAGCCATAATAAAAGAGCTGGACTTTCACATAGGATGAA
TTTCTGGAATGGTTTGATACTTATATTTAACTATTAAAATACCCAGCAGCTATTGGTTTTAATTTAAAAG
TGAGAGTGAATGTCCCCCCAGTCTCTTTTACACAGTATCAAATAGAATAGAAATATTACTATTAGTAATG
GATTCATTGTAGGGAAATGAATGTCCACACCGGAAGCCCTCTTTCTTCCTCTTTCCTTTTCCTTCTTTCC
TGGTCTATTCTTTCCCTTGACAATGTGCTAATGAGCATCTATCTGATATAGTTTAGTGTATATTTTGACA
GAGAATGATATTGTCGGGTGGCCTATTTGGGGGGAAAGAACAACAGGGGAAGGCTCAGCTCAGCTGCTGT
GACTCATCCAAAAACCAGAGCTACCATGAGCAGCAGCAAAAGTGAGGATTTGGTAGGCAGGTACAATTAA
ACCTCAGAAACAAGAATGCTCTGGTTGATGGAATTTTCAGTAACAAGCATAACCTCAATTCCCCCTTTTT
TCCATCTTGATTTTTTAAATAAAAAGTATCTCTTCCTGAATGTGATTTAATGAACATTGTCAATGTCTTC
CTTTGATAACTTAAGGATTTGTTTTTTTCATTGTGAAGATAAATTCTCTTGTCATAGAACGATTAGCTAG
ACACAGAGGGCTGAATATTAGCATGCCATAGCCAAGAAGTACTTTGCATTTCTGGTGTGAAAAATTCCTC
ATATGAGCTTACTTTGTTGTCTTTAGAAAAGTGGAGTGTGGGAGACAAAAAGCTGATGGTGATTTGTGTG
GGAAAACAGATTTTAAAGCTCTTAATCCATTTTGAAGGAATTGACTTACACATTAGCAATCAGTTGCCAT
GTGGATGAGTTGTTATGCTTTTTTTTTTTTTTTACCCTGTAGTTTCAGAAAGCCTAGTGAGAGAGGAATA
TTATTTAGAGAAATCTCAGCATGCGTAAACACTGGTCAGCACTATGAAATGGGAGGGCATCTTGGGGGGA
TAGTAATCCCTTGTCACTGAAGAAAAGTAGAAAGCAGGCTGTAGCAGAACAGAGAACAGATAGTAGAATT
GTTTATCATCTTGGGAGTCTTTGAGATATTAAAAAAATATCTCTTGTCGTTATGTCAAAGGAAGTATCGA
AAGGTGGAAAGATGGAAGAAAGCAGGAAGAGAAAGACCAAAGTCTAGAGGACAATGATGACTTAGGGTTG
CTGTTTTCTTTGAAATAATCTTGAGCACATTTAATAGATTAGACAAGTTTCAGAGGAGGGGATTTAGACT
TGTGTTCCCTCTCCCATGGGGTTTAAAAGACTTTTGCCTACTACTTGGAAGGAGGAGGAAAGAAAGGGTG
TTAGACTGTATCTGGCCTTTCACTGTTAAAGCAACTAGATAGGAGACATTTGATATTTCCCCCACAAAAG
TGAAAACTTGTTTTCCCCACCTTGGTCAAGAAAGTGGAAACAGAAATAGTCCACATGTTTTGGTACATGT
GGTAGTTTTGGTTCAAGACTATTGGAGTTGAGTGGTTATACTAACTGATTGGTCACAGCAGTCATGGGAC
TCAGAGGCAGGGCAAGGAGGGGTGAGTGGTGCTTGGGTCCCCCAGATCCCCCAACAGCCTCTACCTGCTT
GCTCTGGTTCAGCAGTGATTTAACACATCAACCAGCTGAGACTGATGCCCATAGGTGCCCTCTCAGATCA
GGTCATATATTTTGAAAAAGGGTATTATCACTAATCTTTTATAAAATGTTAAAACTTGGTTTCTGTTCCA
ACAACCTAACCATCTCATCTCTCTCTAATTTGTTTCTCTTTAGAAAACATGGTATAAATGCTGAAATACT
TGTGTGTTCTTATTTTCACAGCTTGGAGAGAGTGGAGAGATTAAAGTCATTGGAGAACTGAACTTGCTGT
TTACAACCCTGAAACGTGAATGTGCTCAAGTAGACCAAGGCTGGAAAATGGGTTACTAACCTTTCTCACC
TGCTAAACAATAAGATCCCCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACAAAAAAGATTACT
TTCTATCTCTTTAGAAAAAGCCCCTAAATAACTTCATTTCCATAATCAATACTTTATATTTATGAATTAA
TTTATTGTTATAAGTACATGTCTTATTATTTATGTCACTTTATAATATGGGTTTATTTATGGAAGCATTA
TAAAATATTTCTACTTAATTATAAGGTGAATAGTAATATTTATGATAATAATTATGGAGTTATGTTTATT
TAGTCTCAATAAACACATCGATATCCTAA

Protein Sequence: Show Sequence

>gi|148356272|ref|NP_001091849.1| interleukin-22 precursor [Bos taurus]
MAALQKSVGSPLRDTLAAGCLLVMVLCAQRGAAAPITSHCRLNESDFQEPYIFNHTFTLAQKASLADNIT
DVRLIGNKLFHGIHQVTKRCYVLKQVLNFILEEVLFPQSDKFHPYMEKVVPFFSRLSKKLSQCHVESDNQ
HIQRNVQNLKNTVKKLGESGEIKVIGELNLLFTTLKRECAQVDQGWKMGY

Molecule Role: Vaximmutor
Related Vaccine(s): Mycobacterium avium relA mutant vaccine

6. TGFB1

Gene Name: TGFB1
Sequence Strain (Species/Organism): Bos taurus
NCBI Gene ID: 282089
NCBI Protein GI: 260655603
Locus Tag: BOS_17489
Genbank Accession: EF523342
Protein Accession: NP_001159540
Taxonomy ID: 9913
Chromosome No: 18
Gene Starting Position: 50771353
Gene Ending Position: 50785923
Gene Strand (Orientation): -
Protein Name: transforming growth factor, beta 1
Protein pI: 8.85
Protein Weight: 42126.16
Protein Length: 390

DNA Sequence: Show Sequence

>gi|258513349:50771353-50785923 Bos taurus breed Hereford chromosome 18, Bos_taurus_UMD_3.1, whole genome shotgun sequence
NGCTGGTTGTACAGGGCCAGGACCTGCGGGGCGGGTGCGCGGGGTCAGGGTCGGGGGTCGTGGGGAGGAT
GCCGCCACCCTGCCAGCCTCTCTCCCCATCTGCCTCTCTCTTATCTCATTCCGTGTCTTTCTGGTTCTCT
GCCTCTCCCCATGTTCCACTCTTGGCTTGTATCTCACTGTCTTTCTCCTTCCATCTTCCAAATTCTCTAC
CCTCCTACCTTTTTCTCTCTCATTCTGTCTCCTTTTCTTTCTCCTTCCATCTCCCCACCTCTTCCCTCGC
CCAAGTTGGGATTAAGTCATTTTAGCCGTGTCCGACTCTGTGACCCCATGGACTGTAGCCTGCCAGACTC
CTCTGTCCATGGGGTTTCCCAGGCAAAAATACTGGAGTGGGCCGCCATTTCCTTCTCCAGGGGATCTTCC
TGACCCAGGGATTGAACCCACATCTGTCTCTTATGTCTCCTGCACTGGGAGGTGGGTTCTTTACCACTAG
GTCTCCCCATATCTCTGTCTCCCACTCCCTTGAGTCTCTAACTTTGCAGACCCTGCCTCGAGCTTTTCTC
CCATCCCATTATCACCTCCCCTCCAACCTTCCTCTCCCCTGTATTTCTGACTACCATCTTGCCAATTCAT
CTGATTTGACTTCTCTTTCTCCCTCTCCTCACTCGGTTTCTGCTACCCCCATCCCACCCCCAGCTGGGGA
GCAGGGGGTGTCGGGCTCCAGACGCACCTTGCTGTACTGTGTATCCAGGCTCCAGATGTAAGGGCAGGGC
CCCAGGCAGAAATTGGCGTGGTACCCCTTGGGTTCATGAATCCACTTCCAGCCCAGGTCCTTCCGGAAGT
CAATGTAGAGCTGACGAACACAGCAGTTCTTTTCTGTGGAGCTGCAGGCGGGAGGGAAAAACACTGTCAG
GAGAGCAGAAGGCTGCCCCTCCTCCCCTGGAGTGTGTGTGTTTCACCCTGGTTGCCCTCCAGCATGGTAT
CCAACTTGGTCTCCCTTGCACCCTCTGTGGTAATAACAGCAGTAATAGTAGTGATCATCGCTTACACTGA
TAAAGCACTTCCTCTAGTCCAGCACTGCACTAAGAGCATCCTCATAAGCGTTATCTCAGGCAGCGCTCAA
AACACACCTGTAAGGCACCAAATGAGGAGTTCCCAGGAGGCATCCAGTAGTTAAGACTCCAAGCTTCAAT
ACAACAAAAAGAGAAACAAAAAACAAGCAAACAAAAAAAGACTCCAAGCTTCCACTGTAGGGTGCATGGA
TTCGATCCCTGGGCAAGGAAGTTCCACGTTGCATGCTGCACAGTGCAGCCAAAAAAAAAAAAAAACTAAG
CCTAAAGAAGCCAAATGACTGTCCACAGGGGACAGCTACACAGATCACACTACATCTATACACTGCGACA
TAAACAGCACAGTGCAGGTCCATGTGTACTGATATGTTCTAAGTGTCCAACAAATGTTTACTCAACTACT
GCGTGTTGGGCTGGGGTCTACTGCGGACTCAGTACAAATGAAACAGACAAATCCCTACCTTCTGGGGCCA
CATACTAGAGACTATCATGTTGCCCCTCTGCTCTGAACAAGCCCAATGCCCAAGTCCTCGCCACAAGCTC
AAGGAGCCACCAGATCTAGCTGATCTCAACTCCCTCCCCACTCCCCCTCTCTCACTGTCCTCTGGCCACT
TTAGCATCCTTGCTGTTCCTGAGTCCCAGTCCAGGACCTTTGCACGTGCTGTTCCTTCTGCCTGGCTTGC
TATTCCCACAGATACCCTCTCTGGCTCTCTTTCTTCACTCCATTTATACATGTGCTCAAACATCCTTCAC
TTTGGGGAGTCTTCCCTCCTACAACACTGCCTGGCATGCAGTAGGTGCTCAATAAATATCAGTTAAACAA
ATGACGTCATTGCCTCAGAGACCTTCCTGGATGATTCTATCCAAAACACCAATCTAAAAAATTGTTAATT
CCTAGGGACTTTCTGGGCAGTCCATTGGTAAAGACCCTGTGCTTCCAATGTAGGGGGCATGGGTTCGATC
CCTGGTCAGGAACGATCTCACATGCCATGAGGCACAGCGGAAAAAAAAAAAAAAAGGATAGTTAATTCCA
TTTAGCGTAATAACGGCATTTCAGTTCTGCAAGGAAATATAAAAATGTCTGCAAATGAAAGGGGGCAAAT
CTTAATGCCTGTTGATCTAGATGAGAGTGTATGGGGGTTCATTTTACCATCTTGGTTATACATTTAAATG
TTTCCATAATAAAAAGTTTAAATCAGACTAGGAAAAACAAATCATCACAACGATTTCTTTCTTTCCTCTT
CCTTGGATTTATATTCCTTCTTAGAACTTACTGCCACCTGCTCTTTTATTAGAGATTGATTTGTTCATCT
GTTCATTTCCTGTCTTCCTCTGAGGACAGGACTTTTTGTCTATTTTGTCCCTAAAGGTGTCCCCACTGCC
CATAACAGTCCCCAGCACCTGCCGTGTGTTGGCTGTATACAAGTGTATACAAGTGTTGGCTGTATACAAG
TGTATACAAGTGTTGGCTGTATAAATTAACTGGTCAATGTTACATTTTTCAGAAGCACATCAGAGAGTGG
TGGTGGGTAAGACGACTGCCATGTACTCATGATCTCTGTCCCAGGCTCAGATGCAAAAGTCAGTCCTCGG
GCTTCCCTGGTGGCTCAGTGGTAAAGAATCTGCCTTGCAATGAAGGAGACAGGGGTTTGATCCCTTGTCT
GGGAAGACCCCATGTGCTGTGGAGTAAGTAAACCCTTGTGCCACAACTATTGAGCCTGTGCTCTAGAGCC
CATGTGCTGCAACTACTGTAGTCCCTGCACTCTAGAGCCTGTGCTCTGCAACAAGAGAAGCCACTGCAGT
GAGAAACCCACACACTGCAACTAGAGAAAAGCCTGCACGAATATAATAAAAGTCTGTTTGGAGGGGGACT
GAAGGGTCAGAACTGGAGAGTCTTTGAATATCCTTTCTAGGTTTGGTCCATGTACATGTATTTTCCTATT
TAAAAATCAATACAGAATTTCCTACAAGGCAGGTCTTTTTTTTTTTTTTTTTTTAATATGTATTTGGCTG
TGCCAGGTCTTTGCAGCAACAGGTGAATTCTTTGGTTGCCATATGAAGATCTTAGTTCCCTGACCAGAGA
TCGAACCCAGGCCCCCTGCATTGGGTGTGTGGAGTCTTAGCCATTGGACCACCAGGGAAGTTCCAAGGCA
GGTCTTATTCTATTATTCCCATTTGAAGGAGGAATAAACTGAGCCTTAGAGAAAGGGGGATGTTGTCTGT
TCAAATGTAGGCTGTAGGTGGTAGAGCTCAGGGCCAGAGGTCTCTGACTTCATTTGCCCAGCCCAACCAT
GTCAGCTCAAGCAGAGCGTGAGGAGGCGATGCCACAGGCTCTCCCAGGGCAAGACAAGGCCTTTCCAAGA
GCCACAGGACCTCAAGAAGGAAGTCAGGAAACAGATTCCTGTGAGCTCAGCCTGGTCCCTCCTCAGTCTG
GTTAGGCCTCAGTTCCTTCATCTGTACAAAGATGCAGGGAACGTTAGATCAAATAAGCTATTGTTAAGGG
CCGAGGAGACATCAGGAAGTCCATGGACAGGCTCAGGGAGGGTTCTGAGCTCTCTAAACCATGCACACAG
ATGTGTGTTGTTGTGGTTTAGTTGCTAAGTCATGTCTGACTCTTCTGTGACCCCCTGGACTGTAGTCTGC
CAGGCTCCTCTGTCCATGGGATTCTCCAGGCAAGAATAGTAGAACGGGTTGCCATTTCCTCCTCTGAAGG
ATCTTCCCGACTCAAAGATCAAACCTTTGTTTCCTGTAGCTCCTGCATTGCAGGCGGATTCCTTATCGAT
AAGCTACCAGGGAAGCCCAGATATGTGTGTATGTATAGACATATTCTGGGGGGAAAAGGTTCCTGGCTTT
CAGCTTCTCAAAAAGGTGTTAGATTCACAAAAGGGGAAGAACCACTGTCCAGTAAAATCTATCATTTCTG
GTCCAGCTCTGCTGGGGTGCTGAGATCCTCTGATTCGCAGTGTGTGATTCCGTTACTCAACTCAACTGCT
CTCCACGTGGGGTTCCTGGAGTAGCGGCAGCAGCATCGCCTGGGAACTTGTGGGAGATGCAAGTTCTCAG
GCCCCACCCCAGATGCACTGAATCAGAAACTGGGGGATGGGGATGGCTGGGAGACAGCAAGCCACATTTA
ACAAGCCCTCCAGGTGACCCTGAGCTACTCTCTAACCCTGGATGTTGGGGAAAGGGTCACAGAGGCTTCC
CAGTGGAGATAGTGGTTAAGCTAAGAATAACTTTTTATTGCAGAAAGTCTCGGACATACACCAACCCACT
CCAGTAGTCTTGCCTGGAAAATTCCATGGACAGAAGAGTCTGGCGGGCTACAGTCCACGGGGTCGCAAAG
AGTCAGACGCGACTGAGCACAGCCCAGGCATGGGACATACACAAAAGCAGAGAATCTCACATTTAACTGC
TGTGTCTCTACCATCCAGCTCCAGCGAACATCAAGTCGTGGTCAGTTTCACTGTATTTCTACCCGTATCC
ACTTTCTCTCCTTTGACAAATGATTTTGAAACCCACGTCATCCATCATATTATTTCATCCATAAATATTT
CTATCTCCAAGGAAATCTAGACTACTTTAGAGCATCACACAAAAAATTAACAGTAATTCCTTGATACCAT
CAAATATCTAGTCAGAGTTCATACTTCCCCAACTGTCTCATAAGTATCTTTTTAATTATTTTATTTATCT
ATTTTTTAGCCATACCATGTAGCATGTGGGATCTTAAGTTTCCTGACAAGGGATCAAACCCACATCCTCT
GCATTGGAAGCACAGAGTCTTAAACACTGGACTACCAGGGAAGTCCCAATATCTTTAAAAAAAAAAAAAA
GTTTGGGGAATTCCCTGGTGGTCCAGTGGTTAGGACTCCATGCTTCCAATGCAGGAGACATGGGTTTGAT
CCCTGGTCAGGTTAAGATCCTGGAAACCGTGAAGCAAGGCCAAAAAGCAAATAAGTAAATAAAATTGTAA
AAACAGTCTGCATACATAAAGACTCAAGTAAAGTCTATACAGGGCTATTGGTTGATACATCTTTTAAGAG
TCTTTTAAAAATCTATATAAACCCATCTCTCTATTTCATCCCTTTGTATCGTATTTGTTGAAGAAACTGG
GTCAGGTTTCCCACAATCTAGATGTTGCGGACTGCATGCCCATGGTGGCTGTTTAACATGTTTCTCTGTC
CTTATATTTCTGGAAAATTGGGACTTGGACCTACCATTATTGATGGGCTTCAGGTTTGATTTTTTGGCAA
GACTTCCTAAGTGACACTGTGTCACTCACATGGCTGGCTGCCTTTCTCTTAATGTTATAGCAGGCTGGCA
CTCAGATTTTAATCTATCCATTCGTTCATAGGTTTGTTGGGCAGAGGGTAGGATCTGGTGATGAAGGATC
TTGGAAACAGAGCTGACTTTCCAACAGGAGAAGTGCATGCAACAAAAACCAAAGGCACCATCAAAGTACT
TAGCAGAGACGGGCCAAGGTCGGATTATCATTCTAAATTTGCCATATTCTAACTCTGAATTTCAAAATTC
CAAGACTACAACTTCTCAGTTTTCTTAGACTGCAACATACTGGCATCTCAAGATTCTAACACAGCAACAG
CTTTCCAACTTTCTAGAATTCCCACATCCCCACTCTCGAGGATCTTGGCATTCTGTTTTTTTTTTTGATC
TTGGTATTCTTGAATTCCAATATTTTAAAACTGCATTCCAACATTCCACAGTTCTGAGCCCATCTCCCAA
GAGGTTCAAGCTGCCACTTTCTAAGATCACAGGCTTCCAAGATTCTAACAAGACTCTAGCACTGGGACTT
CCCTGGTGGTCCAGTGGTTAAGAATCCACCTGCCAATGCAGGGGACATGGGTTTGATCCCAGGTCAGGGA
AGATCCCATATGCGCAAGCCGCAATTACTGGAGACAGAGCACCTAGAGCCCATGCTCCTCAACAAGAGAC
GCCTCCTCAACTGAAGAAAGCGCGAGCAGCAGTGAGGATTCAGTGCAATCAAGAATAAATAAATAAGACT
GAGTCCCAACATTCTAAGGTGCCAGTATTCTGGCTTTCACCGACTCCAACATTTTTGGAGGCTAAAAGTT
CAACACAGGATTCCACCAATTCTGGACCTAATATTCAAGCACTCCAACCTGCCAAAATGGGAGAGTCCAG
TCTGTCAATGGTACTCATCATGGGCAATCTTCCAGTTCTGAATTCCCAACTTCTAAGAACCCAACAGTCC
TTGAATCCAACATTCTTTGATTTCAATAATTAATACCAAATTCTAATATCCTAGAATCCCAACGTTTTCA
AACCCCAATCTGCCCACAGTACAGAAATCTAACACGGAGACCTTCCAATACTGAAGTCCCACATGTTCAG
TGTTCCACATTCTAATAAATCTCCGATGCCAACCAGCCAGCAAGAGACCCGCCTGATCTCAACATCCAAG
GACTCCTAGTTTGCACACACTCCAGCATCCCAGGCTCGGACCTCATGCCCTGAACCCTCCAGGCTGAAGG
AGACGCTAGCCCAAGCAGAGCACCAGCCACATGCCCAACCTGTGGTGGCCGCTCAGTGGATGGCGAGGAC
GCCCCCAGCCCAGGAGGCTGCAAGGTTCACCTGAAGCAGTAGTTGGTGTCCAGGGCTCGGCGGTGGCGGG
AGCTGTGCAGGTGCTGGGCCCTCTCCAGAGGGGTGGCCATGAGGAGCAGGAAGGGCCGGTTCATGCCGTG
AATGGTGGCGAGGTCACCCCGGCGGCCGGAACTGAACCCTGCTTTGGTTTTGGGAGTCAGGGGGGTGGGG
AGGATTGGGAATACACACACACACTCAGATGGATGAATGATGAGGATGGGCAGGGGAGGACACACACACA
CAGACACACATCACTGCCGGGCGTCGGCAGGGGTGGGGGGCAGGGAGGGGGCCTCACCGTTAATGTCCAC
TTGAAGCGTGTTATCTTTACTGTCACAGGAACAGTGGGCACTGAGGCGAAAGCCCTCTATTTCCTCTGTG
GGAGTGGGGAGAGGGAAGCCAGCATGAGGGGGGCGGCTCACCCATCCCTGCGGGGGTGGGCAGGAGAGGA
AGGAAGTCGCAAACCCTTGGGGTATGGGGGGATCTCTTTCCTGCCCCTTCCCTTGCCTGACCCCTCACTG
CACCCTCTCACTCCAGTTCTTGGCCTTGCAGCCTCCTTCTCCTCCTCCAGGTCTCAGCGCTGAGGCCACC
TCCTCCAGGAGGCCCTCCAGGACACAGCCCCTCCCCCGACTGTGACAACACGTTTTTTTCTCCAAAGCAC
TGACCTCTGTAGCTCTAACATTTGTCTTTGGCTATTTATTTCCCTCATTGTTTTCAGTGGCGAGCAGGAT
ACTACGGGGGCAGGAAACCTTGTTTTCTTGTTCCCTGCTGGGCCTGGCCCTCCGTCTTAAAAACAAACAT
CTCCTGAGTGCGCTGGGTGCCAGACCCTGTTCTAAACACTCTTTGTGCGTTATTTCCTTTAATCCTCACC
AAAGTTCCCCATTTTACAGATGAGGAAGCCAAGGCCCACAGGCAGGGCTGGGATGGGATGCAAGGACCTA
GGCTCCTGTCCCAAGGCAGGTGCAGAAGAAAGGTTTTACTTCTTTTTTTTTTAATATACAAAAGTAACGG
GTTTTATTAACATTTTTTTGAAAGAATAGTTCGAAAAATGTTAACAAAACCCGTTACTTTTGTATATTAA
AAAGTAGTTAGTCACTCAGTTGTGTCCAACTCTGGGACCCTATGGACTGTAGCTCACCAGGCTCCTCTGT
CCATGGGATTCTCCAGGCAAGAATACTAGAGTAGGTAGCCGTTCTCTTTTCCAGGGCATCTTCACAACCC
AGGGATTGAATCTAGTCTCCTGCATTGCAGGCGGATTCTTTACCATCTGAGTCACCAGGGAAGCCCTTTT
GAAAGAATATGTATATCTAATAATCATAGCAGTATTTATCAGTTGTTCTTATTTTTTAGATTAACTTTTA
TCGGAGTGTAGTTGCTGTACAATGCTGTGTTAGTTTCTGCTGTACAGCAAAGTGAATCAGCTGAATGTAT
ACATACATCCCAGAAGAAAGGTTTTTTGAATGAATGGCTGAATGAATGCTTCCCTAAGCACCCACTATTG
TGTGGGACCCTGTATCAGGTGCTCTGGACTCAGCAGTCAGCTTAGGACACTCGTCCCAGTATGCACCTGC
CCCCGAACTCCCATCCTTTCCTCTGGTCAAACCATCACCTCTTCCATGAGTCTCAACACAATCATTCTCA
CCCAGAAAGCTCTCTTGGATCCCCTCAGAGGGGTTAGGGTGCCCACTCTGGGCTCCTATAGCACTTATGC
CTCCCCCATCCCGGCCCTGCTCACCCTGGCCATCATGGCCTGCTAGGGCTTTGGGAAAGATTTCCTGGGT
ATCTAGAAGTGAGTGCAGGTCCCATGAGGGCAGGCCTAGAGGTTGTCATGGTTACCACTGTGTTTCCCCC
ACCCTACCAGCACAGGGCCAGCCAGAGAGCAGGAACCTACTAGCATTTACCCCAGTGAAGGAACGAATGA
GAGGGAGACTAGGAAGGGAGATGTGAGCACTGGGGCCTTCAACATGACAGGACAGATGGTCCTCTTCCAG
ACAGAACAGGTGTGTCAGGCTTTCCCTAGACTCGAGGCCTCTGCACAGCCTATCCCTCTGCTTTGAACAC
TCTTGCCCCTCCCTCCTTTCCCAGGCTGGCCCCATGAATCCCTCCGACACAGCTCCAATATCCCCTCCTC
CAGGAAGCCCTCCCTGACCTCCCAGGCTGCATCAGGCACCTCCCCTGGGCTCCCCATCCCAACCCTGCCC
ACACTGGGACATTACTACCTGGTTTTCAGGTCTTTCCCCAAGTCCCCCAACCCCAGCAGTGGAACAGGAG
CTTCCCGAAGGTCAGGATGAAGCTGTTTTCGTCACTTCTGTGTCTCCAGCATCACCCAGCACAACGCATG
TGCTCAAGAATGGATTAGCCATTAATCACTCAGGTGTGAGTGTCTGGGAGGAGGGCCAGGTCTGGGCTCT
TTGGCACCAACACAGGGCGGGGGGTGCTAGGCAGGGTGGTCCCAGGACAAAAAATGGGTTACAAAACAGA
GGTGGGGCAGACAAGTGGTCCTCACCTCTGCGGGTCAGCCACTGCCGCACAACTCCAGTGACGTCAAAGG
ACAGCCACTCCGGTGAGTCGCTGGGGGCGAGCAGCCGGTTGCTGAGGTAGCGCCAGGAATTGTTGCTATA
TTTCTGGGGGATGAAGAATTGGGGAGTGAGATAGTGGGTAGAGGCTGCCACAAACACCCATGTACCTTGC
ACCTCTCACTCCATCTGCTCCCCAAAACGGGTCTCCACACAGTCCATCAGTCCCCCTCCCAGACATCCAC
ACTGAGGGGCTTCACAGAAACCACCCAACTCCATGACAGGGGACAGAGAACTCCAGGCCAGGGGTCAAGG
CACCTAGGCATTTCAGCCTCCACCCTTGATCCCGCCATGGCTGTGGAAAATTCCTTTCTCTTTTTTGGCC
TCAGTTTCCTCACTGGTGAAATGCCCAGTTCCTTTCAGCTTGGACAGCTCATGAGGCTAAAATTCTCCTA
CTGACATCACAGAGGTCAATGACGATACAGCTCTGAATTCTTAGAGTCTATCGCTTTCTAGAAGTTATGT
TCGAAGTCATTCCATAACTCCAGCACTTGGTGGCACCCCAAGAATTACACATTCTATGAACTCGAAAAGT
TTTATGAGATTCAAGTTCAACAAACCCAGGAGTCCGACGTGCCAGAAACCTAGACGTTGCAGATGCCTGA
CATCCACGAGGAATGACGCCCTGGGAACTGGCAGCATAAAGCCGAGTTCTCTAGATTCCGAGCAGTTAGT
ATCAAAGCTTCCTCGTCGTAAGAGTCTAACATCTACCACTGAATCTAACACTCCATTTGACAGGGCTTCT
AGGTCACATAGCCTTCGAGAAAATCCAGATTTTTTGGACTCTTCCTGGAGACTCTTCTAGAACAGAGCTA
TCCAAGAGAACTTTCTGTGACGATGCAAATGGGCTTTATCGGTGGTGTCCAAAATAGCAGCCACTCACTC
CATGTGACTACGAGCCACTTGAACTATGGCCAGTGAGACTGAGAAACTGAATTCTTTCTTCTATTTAATT
TTAATCCACTTAAATGGCCATATGTGGCTAGTGGCTACTGGGTGGGCACAGGGCATTTTGAAAGTCTCCA
ACTTTTCAAAGATTCCAACTTTCCTTGTCCTGAGTTTTCAGAGTCCAGCTCCAAAGATTCCAGCTGCTAA
GCATTTAACATTCTAGGGCTCTAGGAGACTCCACACTCTGTGCTCCCTCATCTTTCAGGGTTAGTCCATG
TGGACCCTGCAGCCACCAGCCTTGCGGCCGTCCTGGGACCACCCTAGCCCAGGCCACTTTCCGGGACCCT
TGCCCCCACCTGGTACAGCTCCACGTGCTGCTCCACTTTTAACTTGAGCCTCAGCAGGCGCAGCTCTGCC
CGAGAGAGCAACACAGGTTCGGGCACCGCTTCCCGGAGCTCGGACGTGTTGAAGAACATATATATGCTGT
GCGAGCTAGACTTCATTTTGTCATAGATTTCTAGGCAGAAAGAGTCGAGGGTAGGGGGGCTGTCAGTGTT
TGGCAAGGGCAGGGGAGCTAGTGTACCCCAAATCGAAGAACTCTGGGTTGGACTCCGACCCTGACACTTT
TTAGCTGTGTGACCTTAGGTAACTAGCTTCTCCTCTCTGAGTTTGTTTCCACCTCTTTATTATTATTTTA
AATATTTATTTATTTGGCTGTGCAGGGTCTTAGTTGTCGTGTGGGATCTTCGGGCTTCGTGCAATGTGGG
GTCTTTAGTTGCAGCATGCGAACACTTCGTTGCAGCATGTGAGATCTAGTTCCCTGACCAGGGATGGAAC
CTGGGTCCCCTGCATTGGGAACTTGGAGTCTTAGCCACTGGACCACCAGGGAAATTCCTCTAGCTCTTTA
AACAGGGTCCTATCCTAACCCTCTCATCAGGTAGGAAGTGGCCCCTCAGTACAAAGCTGGCTTGGGGGTG
GGGCTGCTCTGCTTGCTCAGGCCTCCCCACCACCGGGAGCCTCAAATGCAAGTTATGTTGAAGCAGAGAG
ATAAAGGGCACTTGGATCAGACTATGCCTTTGCTGCCTTCTGTGACCTTGGGCAAGTCACATTCCCTCTT
GGAGCTGTTAAATCTACTTGACATCAAGTTCTCTGCCTCCCAACCCAGGCTCTCCTAACTGAAGCGCAGT
GGAACTTTGGGCAGATGAATTCGTCTCTCTTGCCAGATTCTCACCTATAAGCCAAGGACTGATGACAGGA
TGACAGTGTGAAATAGATGAGATGATGCAGATAAAGTATGTCGCACAGAGTCTGCATACAGTGGTTACTT
TAATGTGTGTTCAACAACCCCATGAGAAAGATTCTATTAGTATACCCATTTTATAGATGGGCAAAGAGAG
GAAACAATTTGCCCCTAAGCAACACAGCCAGGAAACTACAGTGTGGGGTGGGGATTCAAACTTGGCCAGT
CTGGTCAGAGCCTATGCTCTTAATCCCTAGGCTATGCTCCCTCCCTGCTAATGTGACATTATTTTTGTTA
TTATTTGTAGTATTTCCTCCCTTAACTGGCTAGAGAAAGTAAAGTTCACTCTAGATAAGGATGGCTTCAG
GGGTGGGGGAAGGGAAACAAAATTAAGAAGACTCCCAGTTCAGGAAAAATTGGGGACTGGGAATGAGGGG
GGCAGGCCATGATCAACTGGAGCAGTTTCGTTAGGCCTCTGCAGTGGGGGCATGGAAATGGTGGAGACAT
GTTGAGAAAAGACAGAGGAAGACCCTCTTGAGCGCCTCCTTCCCCCTGCTCCTCCCGTCTCTTGGTCTCT
TGCCGGGCTGGCCCCTTTCCCTCACGAATAAGCTCTATCTTGGCCCAAGAGTTACTCAGCCGACCCAAAC
GGAAGGGCAGAGCCCAGGCCCAGGAGACAGGGAGGAAGAGCGATAGAGGGAGCAAGAAGGGTCAGAGTGA
GAGATGGAAGTAGGATATTGAGGAGAAAAAGAGAGAAAAAGGGAAGAGATGGAGAGAGAAACTAGGAGAC
CCAGAGAGGGTCAGAGTTACACGGATGCGGGAGAGACAGAAAGGCAGAAGTCAAACGGGAAGACAGACGG
AGGGTTTGGGAGCGAGATGGAGGCAGAAGAGGGCTGAAGAGCAACTGAAGGTGTCAACATTGGAAAAACC
TCTCCAACGAAGGTGAAGCGTCGACGGAAAAGGTGAGACGGAGATGTGGGTGGGTAGGCTGTAGGGATGT
CATCTGACTGAAGCCGCAACTGGGCGCCCAAATGGGCCAGAGACGCACACGTCTACTGCCCACGAGGGAA
ACTAAGAGCGGGGGGCGGGGTTCGCCCCCGAGCCTCGGGGCCCAGCCGGACGCCTGGGTACCCGCGCGGG
CGCCGACAGGGCGGGCCCAGGGTAGCCCCGCCCTGAGCTAGGGGTGAAGAGGGCGGGAGTCGCCGCCGTC
GCGAGGGCGGGGACCGGACCTGCCTCGTCTCGCCCCAGGCTCAGCGCGCGATCCCCGCCCCGTAACCCTC
CGCCCCCAACCCCGAAGCACAAGAAGGAAAGGGAAGGGAGGGCTAGGCAGCCGGACGCTGGGGTTTCCCC
AGCCTCGCTGAGAGGAACTAGGAGCTTGGGGTCTACGCTCTCCGCAGAGCTCCGCGGGGTCCTCCTGTTC
CTTCCGGAAGCGCAGGCTCCTCCCCCACACCCGCGCGTGGCACCCACGTGGGGCTTCTCCAACCCCTACA
AGCTCGGTCTTTCCCCCGGGTCTCGCCGAGTCGCATTCACCCAGCTCTCTCCCTACTACGACTCCTCACT
CAGCCCTCTTCCTTCCAGCCAGCGGCGAGAGGGTGGCGGGCCCTGCTGCTCCCAGCATGACACCATCCGT
CGCTCCTCTACCCCATTCCAGGTCCACCTTTCCCACGCTTATCCTCCCGTTCTGTAAACCCCTTCCCACG
ACCCAGTATTGTAGGTTTCCTGGGAGCGCGCGCTTCTTTCTCTGGGTTTCATCCTCTTACAGTCCTTGTG
GGAAATATTAAAAGCACCTCCCGGCGCATTCTGGACACCCCCCGCCCCCACCCCCTGGGCACATCCTAGG
GTCTCCGCTTCTCTCCCGCTAGTTTCCCGCACCCTACAATCTCGCGTCCCCTTCCGACCTCACATTTATC
ACCCTCTCCTTCTAAGCCCTCCTCCAATCTCGCGTGTTCTTCGCGTCCTGTGCATGCGGGTCCACTCTCT
CTACTTTCCTGGCACCCCTCTGGGATTCGCTTCTGTTTTTCTGTCCTTGAATCTTCCTGCCTCACGGCCC
CTGAACTCTGAGTCAGCTTTCCCCACTACCTCTCCTTTTCCAAATGCCGGGGTGCTAGTTTCTCCCAAAG
CGAGATCCCTCTCTGCAGCCCCGCTCCCACGACCCCCTGCATTCTCGAGCACCCTGCAAGGGGAAGTCTT
TTTCGAATGGCTTCGCTTCTCTCACTCTAAGGGACTCCCCACACGATCTCGCATGATCCCGTCGCCCTCT
AAAAGCGGTCCACTTCGCTCACTATCTCTTATTCAAGACCAGATACCTTGGGGTTAGGGAGCTCAGCGCT
ATCTCTTTGGGACCCCTCCCCCCGACACGTTCCCGGCGCCCCCGGGGGTTCTCTTCCTCCAGCCAGTTTC
CTCTACCAGCAGTTTCCTCCCCGTGACCCCTGCACTCCGGCGCCCCCTGGGGGCTCCCCTCCAGGGTCCC
CTGCCCGTGCGAGCTCACTGTTGCCGTATTCCACCATTAGCACGCGGGTGACCTCCTTGGCGTAGTAGTC
CGCCTCTGGCTCAGGCTCCGTTTCGGCACTTTCCCCGGCCACCCGGTCGCGGGTACTGTTGTAAAGGGCC
AGTATGGCCTCGGGCAGCGGGCCGGGTGGCACATCCCCCTGGCTCGGGGGGCTGGCGAGCCGAAGCTTGG
ACAAAATCTGGCCGCGGATGGCCTCGATGCGCTTCCGCTTCACCAGCTCCATGTCGATGGTCTTGCAGGT
GGACAGTCCGGCGACCGGCCGGCCAGGCGTCAGCATTAGCAGCCACAGCAGCGGCAGCAGCAGCGGCAGC
AGCCGCAGCCCCGAGGGCGGCATGGGGGAGGCGGCGCCCCCCGGCACAGCCGAGAGCGCGAGCTGGGCTG
GGGTGGGAAGAGGGAGGCCCCGCCCCTACAGGGGCTGGGGTGGGTGGGGGTCTCCCGAGGAAAAGGTAGG
AGGGTCTGTTTGCGGGGAGAGTTGAGGCCTTCAGGGAGAACGGAGCAGAGCTGGAGGGGAGGCTCTGATG
GGTGGGTTCTCGGTATCCCACGGAAAAAAAACCGAGATGGGTGAGCTCTGGTACTAGAGATGGGTGATCT
TGAATAGGAAAGCTGAGGCAAGTCTGAGAGAGATCCCGGATCCCGGAGAAGGGTCCTCGGATGCGCGGGG
GCTCAGGAGAG

Protein Sequence: Show Sequence

>gi|260655603|ref|NP_001159540.1| transforming growth factor beta-1 precursor [Bos taurus]
MPPSGLRLLPLLLPLLWLLMLTPGRPVAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPP
GPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSEL
REAVPEPVLLSRADVRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTR
REEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLMATPLERAQHLHSSRHRRAL
DTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGA
SAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Molecule Role: Vaximmutor
Related Vaccine(s): Mycobacterium avium relA mutant vaccine

IV. Vaccine Information

1. M. avium DNA vaccine DNA-Av35/p2AIL12

a. Vaccine Ontology ID:

VO_0004540

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Antigen

M. avium 35,000 MW protein (Martin et al., 2003)

f. Vector:

pJW4303 (Martin et al., 2003)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Immune Response: Co-immunization with p2AIL-12 and DNA-Av35 led to a significant increase in the number of antigen-specific IFN-gamma secreting cells and total amount of IFN-gamma released, but a concomitant fall in the antibody response to the 35000 MW protein (Martin et al., 2003).
Efficacy: Immunization with DNA-Av35 resulted in significant protective immunity against M. avium infection with a 2-log10 reduction in the M. avium load in the spleen (P < 0.001). The addition of p2AIL12 to the DNA-Av35 vaccine significantly increased the level of protection in both the spleen (P < 0.005) and liver (P < 0.01), with a further reduction in the bacterial load of about 1-log10 (Martin et al., 2003).

2. Mycobacterium avium relA mutant vaccine

a. Vaccine Ontology ID:

VO_0002779

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Goat

e. Gene Engineering of relA

Type: Gene mutation
Description: This relA mutant is from Mycobacterium avium (Park et al., 2011).
Detailed Gene Information: Click here.

f. Immunization Route

Oral immunization

g. Goat Response

Persistence: A relA mutant is attenuated in goats (Park et al., 2011).
Efficacy: A relA mutant induces protection in goats from challenge with wild type M. avium (Park et al., 2011).
Host Gene Response of IFNG
- Gene Response: Expression of IFN-gamma was increased. Specifically, a quantitative RT-PCR revealed the gene encoding IFN-γ was up-regulated in PBMC stimulated with live Map (a M. avium relA mutant) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of IL10
- Gene Response: Expression of IL-10 was decreased. Specifically, a quantitative RT-PCR revealed the gene encoding IL-10 was down-regulated in PBMC stimulated with live Map (a M. avium relA mutant) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of IL17A
- Gene Response: Expression of IL-17 was increased. Specifically, a quantitative RT-PCR revealed the gene encoding IL-17 was up-regulated in PBMC stimulated with live Map (a M. avium relA mutant) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of IL22
- Gene Response: Expression of IL-22 was increased (Park et al., 2011).
- Detailed Gene Information: Click here.

h. Cattle Response

Description: Cattle peripheral blood mononuclear cells (PBMC) were used for cytokine detection (Park et al., 2011).
Host Gene Response of IFNG
- Gene Response: Expression of IFN-gamma was increased in cattle PBMC. Specifically, a quantitative RT-PCR revealed the gene encoding IFN-γ was up-regulated in PBMC stimulated with live Map (WT) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of IL17A
- Gene Response: Expression of IL-17 was increased in cattle PBMC. Specifically, a quantitative RT-PCR revealed the gene encoding IL-17 was up-regulated in PBMC stimulated with live Map (WT) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of IL22
- Gene Response: Expression of IL-22 was increased in cattle PBMC. Specifically, a quantitative RT-PCR revealed the gene encoding IL-22 was up-regulated in PBMC stimulated with live Map (WT) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
- Detailed Gene Information: Click here.

V. References

1. Martin et al., 2003: Martin E, Kamath AT, Briscoe H, Britton WJ. The combination of plasmid interleukin-12 with a single DNA vaccine is more effective than Mycobacterium bovis (bacille Calmette-GuÃƒÂ¨rin) in protecting against systemic Mycobacterim avium infection. Immunology. 2003; 109(2); 308-314. [PubMed: 12757627].

2. Park et al., 2011: Park KT, Allen AJ, Bannantine JP, Seo KS, Hamilton MJ, Abdellrazeq GS, Rihan HM, Grimm A, Davis WC. Evaluation of two mutants of Mycobacterium avium subsp. paratuberculosis as candidates for a live attenuated vaccine for Johne's disease. Vaccine. 2011; ; . [PubMed: 21565243].

3. WebMD - Mycobacterium avium complex: HIV, AIDS, and Mycobacterium Avium Complex [http://www.webmd.com/hiv-aids/guide/aids-hiv-opportunistic-infections-mycobacterium-avium-complex]