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Chlamydia muridarum

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Introduction
    3. Host Ranges and Animal Models
  2. Vaccine Related Pathogen Genes
    1. atpA (Protective antigen)
    2. MOMP (Protective antigen)
    3. pGP4-D (Protective antigen)
    4. pmpE (Protective antigen)
    5. PmpE/F-2 (Protective antigen)
    6. PmpG-1 (Protective antigen)
    7. pmpH (Protective antigen)
    8. RplF (Protective antigen)
    9. Tarp (Protective antigen)
    10. TC0439 adherence factor (Protective antigen)
    11. TC0512 outer membrane protein, putative (Protective antigen)
    12. TC0757 hypothetical protein (Protective antigen)
    13. TC0767 hypothetical protein (Protective antigen)
    14. TC0768 hypothetical protein (Protective antigen)
    15. Virulence plasmid protein pGP3-D (Protective antigen)
  3. Vaccine Related Host Genes
    1. Ifng (Interferon gamma)
    2. IgA
    3. IgG
  4. Vaccine Information
    1. C. muridarum DNA vaccine encoding TC0439
    2. C. muridarum DNA vaccine encoding TC0512
    3. C. muridarum DNA vaccine encoding TC0757
    4. C. muridarum DNA vaccine encoding TC0767/768
    5. C. muridarum MOMP protein vaccine
    6. C. muridarum PmpE/F-2 protein vaccine
    7. C. muridarum PmpG-1 protein vaccine
    8. C. muridarum PmpG-1, PmpE/F-2, and MOMP Proteins Vaccine
    9. C. muridarum RplF protein vaccine
    10. C. muridarum Tarp subunit vaccine
  5. References
I. General Information
1. NCBI Taxonomy ID:
83560
2. Introduction
Chlamydia muridarum is an intracellular bacterial species that at one time belonged to Chlamydia trachomatis. However, C. trachomatis naturally only infects humans and C. muridarum naturally infects only members of the family Muridae (includes both mice and hamsters, Alderton, 1996). Two strains of Chlamydia muridarum, MoPn (originally named Nigg) and SFPD, have been isolated from mice and hamsters. Glycogen production by both strains has been demonstrated, although it is difficult to detect glycogen in MoPn (Moulder et al., 1984). Chlamydia muridarum MoPn binds mAbs recognizing Chlamydia trachomatis MOMP vs4 core epitope (T)LNPT(IA). DNA sequence analysis indicates that these mAbs should recognize SFPD and that Chlamydia trachomatis B-serogroup mAbs specific for the vs4 epitope IAGAG should recognize SFPD (Wiki: Chlamydia muridarum).
3. Host Ranges and Animal Models
C. muridarum naturally infects only members of the family Muridae (which includes both mice and hamsters) (Wiki: Chlamydia muridarum).
1. atpA
  • Gene Name : atpA
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • NCBI Protein GI : AAF39416
  • Other Database IDs : CDD:235248
  • Taxonomy ID : 243161
  • Protein Name : ATP synthase, subunit A
  • Protein pI : 4.79
  • Protein Weight : 61088.64
  • Protein Length : 669
  • Protein Note : V-type ATP synthase subunit A; Provisional
  • Protein Sequence : Show Sequence
    >AAF39416.1 ATP synthase, subunit A [Chlamydia muridarum str. Nigg]
    MVATSKQTTQGYVVEAYGNLLRVHFDGHVRQGEVAYVSVDDTWLKAEIIEVVGDEVKVQVFEETQGISRG
    ALVTFSGHLLEAELGPGLLQGIFDGLQNRLEVLADTSLFLKRGEYVNAICRETVWAYTQKASVGDVLSRG
    DVLGTVKEGRFDHKIMVPFSCFEEVTITWVISSGDYTVDTVIAKGRTASGAELEFTMVQKWPIKQAFLEG
    EKVPSHEIMDVGLRVLDTQIPVLKGGTFCTPGPFGAGKTVLQHHLSKYAAVDIVVLCACGERAGEVVEIL
    QEFPHLTDPHTGQSLMHRTCIICNTSSMPVAARESSIYLGITIAEYYRQMGLHVLLLADSTSRWAQALRE
    ISGRLEEIPGEEAFPAYLASRIAAFYERGGAVKMKDGSEGSLTICGAVSPAGGNFEEPVTQATLSVVGAF
    CGLSKARADARRYPSIDPMISWSKYLDSVAEILEKKVPGWGDSVKKASRFLEEGAEIGKRIEVVGEEGIS
    MEDIEIFLKSELYDFCYLQQNAFDAEDCYCPFDRQIELFSLMSHIFSSRFCFDCPDNARSFFLELQSKIK
    TLNGQKFLSEDYQKGLEVIYKLLESKMVQTA
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Tifrea et al., 2016)
2. MOMP
  • Gene Name : MOMP
  • VO ID : VO_0010890
  • NCBI Protein GI : 1518659
  • Other Database IDs : CDD:279628
  • Taxonomy ID : 83560
  • Gene Strand (Orientation) : ?
  • Protein Name : major outer membrane protein
  • Protein pI : 4.86
  • Protein Weight : 39872.84
  • Protein Length : 457
  • Protein Note : mouse pneumonitis; submitted as Chlamydia trachomatis
  • Protein Sequence : Show Sequence
    >AAB07068.1 major outer membrane protein [Chlamydia muridarum]
    MKKLLKSVLAFAVLGSASSLHALPVGNPAEPSLMIDGILWEGFGGDPCDPCTTWCDAISLRLGYYGDFVF
    DRVLKTDVNKQFEMGAAPTGDADLTTAPTPASRENPAYGKHMQDAEMFTNAAYMALNIWDRFDVFCTLGA
    TSGYLKGNSAAFNLVGLFGRDETAVAADDIPNVSLSQAVVELYTDTAFAWSVGARAALWECGCATLGASF
    QYAQSKPKVEELNVLCNAAEFTINKPKGYVGQEFPLNIKAGTVSATDTKDASIDYHEWQASLALSYRLNM
    FTPYIGVKWSRASFDADTIRIAQPKLETSILKMTTWNPTISGSGIDVDTKITDTLQIVSLQLNKMKSRKS
    CGLAIGTTIVDADKYAVTVETRLIDERAAHVNAQFRF
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Female BALB/c mice were immunized with chlamydial major outer membrane protein (MOMP) mixed with cholera toxin and CpG oligodeoxynucleotide adjuvants by either the IN or TCI routes. Both intranasal and transcutaneous immunization protected mice against respiratory challenge with Chlamydia (Skelding et al., 2006).
  • Related Vaccine(s): C. muridarum MOMP protein vaccine
3. pGP4-D
  • Gene Name : pGP4-D
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • NCBI Protein GI : Q46440
  • Other Database IDs : CDD:235774
  • Taxonomy ID : 243161
  • Protein Name : Virulence plasmid protein pGP4-D
  • Protein pI : 9.61
  • Protein Weight : 13082.5
  • Protein Length : 176
  • Protein Note : Virulence plasmid protein pGP4-D. /FTId=PRO_0000218345.
  • Protein Sequence : Show Sequence
    >sp|Q46440.1|GP4D_CHLMU RecName: Full=Virulence plasmid protein pGP4-D
    MQNKRKLRNDFIKIVKDVEKDFPELDLKIRVNKERVTFLNSPLELYHKSISLILGLLQQIEKSLELFPDS
    PVLEKLEDNSLKLKKALIMLILSRKDMFSKAE
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation :
4. pmpE
  • Gene Name : pmpE
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • NCBI Protein GI : Q9PL47
  • Other Database IDs : CDD:330218
    CDD:311484
    CDD:214872
  • Taxonomy ID : 243161
  • Protein Name : Probable outer membrane protein PmpE
  • Protein pI : 6.79
  • Protein Weight : 105209.61
  • Protein Length : 1130
  • Protein Note : Polymorphic membrane protein E
  • Protein Sequence : Show Sequence
    >sp|Q9PL47.1|PMPE_CHLMU RecName: Full=Probable outer membrane protein PmpE; AltName: Full=Polymorphic membrane protein E; Flags: Precursor
    MKKLFFFVLIGSSILGFTREVPPSILLKPILNPYHMTGLFFPKVNLLGDTHNLTDYHLDNLKCILACLQR
    TPYEGAAFTVTDYLGFSDTQKDGIFCFKNLTPESGGVIGSPTQNTPTIKIHNTIGPVLFENNTCHRLWTQ
    TDPENEGNKAREGGAIHAGDVYISNNQNLVGFIKNFAYVQGGAISANTFAYKENKSSFLCLNNSCIQTKT
    GGKGGAIYVSTSCSFENNNKDLLFIQNSGCAGGAIFSPTCSLIGNQGDIVFYSNHGFKNVDNATNESGDG
    GAIKVTTRLDITNNGSQIFFSDNISRNFGGAIHAPCLHLVGNGPTYFTNNIANHTGGAIYITGTETSKIS
    ADHHAIIFDNNISANATNADGSSSNTNPPHRNAITMDNSAGGIELGAGKSQNLIFYDPIQVTNAGVTVDF
    NKDASQTGCVVFSGATVLSADISQANLQTKTPATLTLSHGLLCIEDRAQLTVNNFTQTGGIVALGNGAVL
    SSYQHSTTDATQTPPTTTTTDASVTLNHIGLNLPSILKDGAEMPLLWVEPISTTQGNTTTYTSDTAASFS
    LNGATLSLIDEDGNSPYENTDLSRALYAQPMLAISEASDNQLQSESMDFSKVNVPHYGWQGLWTWGWAKT
    ENPTTTPPATITDPKKANQFHRTLLLTWLPAGYIPSPKHKSPLIANTLWGNILFATENLKNSSGQELLDR
    PFWGITGGGLGMMVYQEPRKDHPGFHMHTSGYSAGMITGNTHTFSLRFSQSYTKLNERYAKNYVSSKNYS
    CQGEMLLSLQEGLMLTKLIGLYSYGNHNSHHFYTQGEDLSSQGEFHSQTFGGAVFFDLPLKPFGRTHILT
    APFLGAIGMYSKLSSFTEVGAYPRTFITETPLINVLIPIGVKGSFMNATHRPQAWTVELAYQPVLYRQEP
    SISTQLLAGKGMWFGHGSPASRHALAYKISQKTQLLRFATLQLQYHGYYSSSTFCNYLNGEVSLRF
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Yu et al., 2009)
5. PmpE/F-2
  • Gene Name : PmpE/F-2
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • VO ID : VO_0010893
  • NCBI Gene ID : 1246432
  • NCBI Protein GI : 497915819
  • Locus Tag : TC_RS01330
  • Genbank Accession : AE002160
  • Protein Accession : WP_010229975
  • Taxonomy ID : 243161
  • Gene Starting Position : 308276
  • Gene Ending Position : 311353
  • Gene Strand (Orientation) : -
  • Protein Name : autotransporter domain-containing protein
  • Protein pI : 8.26
  • Protein Weight : 107782.38
  • Protein Length : 1025
  • Protein Note : similar to GP:4376753; identified by sequence similarity; putative
  • DNA Sequence : Show Sequence
    >NC_002620.2:308276-311353 Chlamydia muridarum Nigg, complete genome
    GTTAAAAGACCAGAGCCCCTCCTGCATTCATGTAGTGTGAGACTGTAGAAGTAGCCACCTGCGCTTGATA
    ATTAGCGAATAGTTTAAGATAAGAGAATTTTAGAGAAGATGATCCTCTCCCATAAAAGGAATGCTTAGCT
    AAAGGAGTGTTTGTTGTTTCCCAAGAACCATTGTTTTTGATTAAAACGGTATTAAGAATGGGGCGCTTCC
    AGTAAAGAGTAGGCTGGTATGCTACCTCCATTTCGCAGGATACAGTTGGCCATTTATTCGAAGAATAAAC
    ACCTTTAATTCCTATTGGAGAAACAACAGCTGTATGTGGTTGTTTTAACGAAAATAATCTAGCCAGGTCT
    CCCTGCTCCTGGATAGATGCAGGCTCTGTTCGGGAGATGAGAGCTTGAATGAATGGTGTCAACATAATCG
    ATTGGAAAGGCATACTATCCCGCAGTTCGCAACGTAGAGAGCCCCCCAGAGTCGTAGAGTGAAAGTTCCC
    TTTAGAAAACTTTTTCGTTCCATAGAAGTTATATGTTTTATGAGTACCATAGTTATAAGCAGCTGCTCCA
    ATAAGAGCGATACGATCAAACATAGGTTGTTCAAAACACAGAGCGGAGAGATAATAACGAGAAGAAATTT
    TATTATTGGTACGTTTCTCTTTCATTGTGTCGGAGGATTGGGAAAAGGAAAATAAAAACTTATGTCCGTG
    TGCTCCTGATGCTTGTGAAGATATAGCATATCCTTTCGATGCAGAAGAATATCCGTGATAATCTAAGCGT
    TTCTGCTGTTTGGTGAATAGCGTAGCTGCAGATCCTTGTAAAGCGAACCAAGAGTCATCTGGTAGTAATG
    AACGTAAGTACAGGGTTGTATTTCTTCCAGAAGACACTAAATTATTCGCAATAAGATCCCCTCTACGAGC
    AGGATCTGCTATATATCCGATTGGGGTCCAGCTCACTACTAAGGAGTGTCTAGTGACTGTTACGTCCGTT
    GTAGCAAGAGGAGTTGTTCCTCCTGTAGAAGCTCTAATGGAAGGAATCTTAGCTGTAGTTGTACTGTTGG
    AATCAAAAGTTTCGCTAGTGTTTTTTTGTTCTTGAATAGAATTGTTGGCTGTTTGAGTCTCTGTACTATT
    GGTAAGAGAGACCGTACGGGTGGTTGTCATCCAATTAGATGTCCAGAGTCCTTGGTATCCGTAATGTGGT
    AAAGAATATAGCGAGGTATTGAGAGTGTTTTTCCAATCATTGCTTAACTGCGCATCATTAGTCGTTTCGA
    TATGTAAAATGGGTTGATTTGCTTTTCCTCGGGATAGGTCGCAGCTGTCATAAACTGATTCGTAATTTTC
    ATCTACTAACGAGACAAGACCGGAGAAAAAGATCTTTTGAGTTGCCACGTTCTCTGTGCTTACTGGAGCT
    TGGTTAGAGTTTGTATTCTGTGTCATATCTTGGGGATTAACACGAATACGTGGAGCTGTAGTACCTTGGT
    CATTGAGAAGACCTGGCAAAATGATTCCAAGATGCGTAATAGTGAGAGAAGCGTCTTTCCCTACAGTAAG
    AATAGAATCTTGTCCTAAAGCAAGAAGGCTAGTGCTTTCTTGAGTAAGAGGGCCATTGAAAAATTCAAAC
    TCTGCGCCATTTTCTATGGACATAGTCCCAAACTTCAAGGTAGTGGGGGAGTCTTTAATTTGGTTATGAG
    TTTTACTGGCAATCATGTGAGCTTGTAGATCACTGGATATGTTTTTGTATGAAAACACAACAGCGCCAGA
    GAACTCATCCGAAGGATTGATGGTAACGGTGTGCGGGCTATTTTCAGGGGGTGTATTAGCTTTTTTTAAG
    CCTCCCCATTGAAAAAGATCATAAAAATAGATGCGAGTATCTTTCTTAGCACCAAGAGATAGGGTGATAG
    GGTTATTGTTGGTTGTTCCAGGAATATAAAAAAGTTTTCGCAAACCTGCTGACATTCCGGTAGTTGAAAG
    CTCTGTATTATTGTTGAAGATAATATCCCCAGAGCCAGTATGAATATAGTTCGCAGCTGGGCTTGCAAAT
    AGGTGAAAAGCTCCTCCATACAGAGCCGAATTATTAAGAAAATAGATAGGACCATTTTCTTGAAAATTAA
    GGTTGTTGCAGGAAATAGCTCCTGCTATGTATGAGGCATTATTATTCTCAAAGTAGATAGGGCCTTTATT
    ATTTAGAAGACCAAACTCTCCTCCTGCGCTTATAACTGCTCCATTTCCTTGAGCACTAGTGTTATGCCAT
    CCTAAAGCGCTATTATTTATAAAAATAAGCTTATCCTTATTATCTCTAAAAATACACTGTCCCCCGGTGC
    TGATAGCTCCTCCGTATCCTCCATAGTTGGAATTGAAGACTACCGGACCAGTATTGTGTGAGATTTCGCA
    GGCACCTTGGCAGCCAACAGCTCCTCCTCGAGCAAGAATTGTAGCAAATGTGTTGGGTATGTGATCAGTA
    GTATGGTTATTTTCAAAAGCAACTATTCCGGACGTATTTTTGATAACTAGGTTTTGGATTGTGGATACGG
    CTCCTCCCATATCCCCAGAAGTGTTATTGATAAAATTTAAGCTATGATTATTAATTAGCTCGATGCTTGA
    TCCGTATAAAGCCCCTCCGCGATTCCTTTCGGTTCTTAATGCTACTCCTGTAAATTGAGTTCCAATAGTT
    GTATTATTATAGAACAATATGTTTTGGTTGTTTTTAAAGCTAATAAGGCCTGTAGCATTTATAGCTCCCC
    CTCCTCCCTGATGGGTGCAATAATTGTTACAAAAAAACAAGTCTGCATTGTCAATGGCTGTGAAAGAAGT
    CATAGACTCAACAAAAGCTCCACTAGAGTTTGTAGTGCTTAAACCTCTCGAAAACCATTGATCCTGCGTA
    CAACTTGAAATAGTAAAGTTCCCTCTATAATTAGAAACATTTGCGTAAGTGATGTATTGAGCAGCAATGG
    AGCTAGGCGGAGTAACTATGAATTCAATCTCTCTACCACCAAAAGTCAAGTTCGGAAGTTTGAGTGTATC
    GGTTTCACTGGCCGAAATACAAGATAAAGGAATGAAAACAAGTGAAAGAGGGAGAATTCTGCGAGTCA
    
    
  • Protein Sequence : Show Sequence
    >WP_010229975.1 autotransporter domain-containing protein [Chlamydia muridarum]
    MTRRILPLSLVFIPLSCISASETDTLKLPNLTFGGREIEFIVTPPSSIAAQYITYANVSNYRGNFTISSC
    TQDQWFSRGLSTTNSSGAFVESMTSFTAIDNADLFFCNNYCTHQGGGGAINATGLISFKNNQNILFYNNT
    TIGTQFTGVALRTERNRGGALYGSSIELINNHSLNFINNTSGDMGGAVSTIQNLVIKNTSGIVAFENNHT
    TDHIPNTFATILARGGAVGCQGACEISHNTGPVVFNSNYGGYGGAISTGGQCIFRDNKDKLIFINNSALG
    WHNTSAQGNGAVISAGGEFGLLNNKGPIYFENNNASYIAGAISCNNLNFQENGPIYFLNNSALYGGAFHL
    FASPAANYIHTGSGDIIFNNNTELSTTGMSAGLRKLFYIPGTTNNNPITLSLGAKKDTRIYFYDLFQWGG
    LKKANTPPENSPHTVTINPSDEFSGAVVFSYKNISSDLQAHMIASKTHNQIKDSPTTLKFGTMSIENGAE
    FEFFNGPLTQESTSLLALGQDSILTVGKDASLTITHLGIILPGLLNDQGTTAPRIRVNPQDMTQNTNSNQ
    APVSTENVATQKIFFSGLVSLVDENYESVYDSCDLSRGKANQPILHIETTNDAQLSNDWKNTLNTSLYSL
    PHYGYQGLWTSNWMTTTRTVSLTNSTETQTANNSIQEQKNTSETFDSNSTTTAKIPSIRASTGGTTPLAT
    TDVTVTRHSLVVSWTPIGYIADPARRGDLIANNLVSSGRNTTLYLRSLLPDDSWFALQGSAATLFTKQQK
    RLDYHGYSSASKGYAISSQASGAHGHKFLFSFSQSSDTMKEKRTNNKISSRYYLSALCFEQPMFDRIALI
    GAAAYNYGTHKTYNFYGTKKFSKGNFHSTTLGGSLRCELRDSMPFQSIMLTPFIQALISRTEPASIQEQG
    DLARLFSLKQPHTAVVSPIGIKGVYSSNKWPTVSCEMEVAYQPTLYWKRPILNTVLIKNNGSWETTNTPL
    AKHSFYGRGSSSLKFSYLKLFANYQAQVATSTVSHYMNAGGALVF
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. In addition, pmpE/F-2 was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
  • Related Vaccine(s): C. muridarum PmpE/F-2 protein vaccine
6. PmpG-1
  • Gene Name : PmpG-1
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • VO ID : VO_0010891
  • NCBI Gene ID : 1246433
  • NCBI Protein GI : 497915821
  • Locus Tag : TC_RS01335
  • Genbank Accession : AE002160
  • Protein Accession : WP_010229977
  • Taxonomy ID : 243161
  • Gene Starting Position : 311528
  • Gene Ending Position : 314488
  • Gene Strand (Orientation) : +
  • Protein Name : Pmp family polymorphic membrane protein autotransporter adhesin
  • Protein pI : 5.8
  • Protein Weight : 98285.26
  • Protein Length : 986
  • Protein Note : similar to GP:4376736; identified by sequence similarity; putative
  • DNA Sequence : Show Sequence
    >NC_002620.2:311528-314488 Chlamydia muridarum Nigg, complete genome
    GATGCAAACGCCTTTTCATAAGTTCTTTCTTCTAGCAATGCTATCTTACTCTTTATTGCAAGGAGGGCAT
    GCGGCAGATATTTCCATGCCTCCGGGAATTTATGATGGGACAACATTGACGGCGCCATTTCCCTACACTG
    TGATCGGAGATCCCAGAGGGACAAAGGTTACTTCATCGGGATCGCTAGAGTTGAAAAACCTGGACAATTC
    CATTGCGACTTTACCTCTAAGTTGTTTTGGTAATTTGTTGGGGAATTTCACTATTGCAGGAAGAGGGCAT
    TCGTTAGTATTTGAGAATATACGAACATCTACAAATGGGGCGGCATTGAGTAATCATGCTCCTTCTGGAC
    TGTTTGTAATTGAAGCTTTTGATGAACTCTCTCTTTTGAATTGTAATTCATTGGTATCTGTAGTTCCTCA
    AACAGGGGGTACGACTACTTCTGTTCCTTCTAATGGGACGATCTATTCTAGAACAGATCTTGTTCTAAGA
    GATATCAAGAAGGTTTCTTTCTATAGTAACTTAGTTTCTGGAGATGGGGGAGCTATAGATGCACAAAGTT
    TAATGGTTAACGGAATTGAAAAACTTTGTACCTTCCAAGAAAATGTAGCGCAGTCCGATGGGGGAGCGTG
    TCAGGTAACAAAGACCTTCTCTGCTGTGGGCAATAAGGTTCCTTTGTCTTTTTTAGGCAATGTTGCTGGT
    AATAAGGGGGGAGGAGTTGCTGCTGTCAAAGATGGTCAGGGGGCAGGAGGGGCGACTGATCTATCGGTTA
    ATTTTGCCAATAATACTGCTGTAGAATTTGAGGGAAATAGTGCTCGAATAGGTGGAGGGATCTACTCGGA
    CGGAAATATTTCCTTTTTAGGGAATGCAAAGACAGTTTTCCTAAGTAACGTAGCTTCGCCTATTTATGTT
    GACCCTGCTGCTGCAGGAGGACAGCCCCCTGCAGATAAAGATAACTATGGAGATGGAGGAGCCATCTTCT
    GCAAAAATGATACTAACATAGGTGAAGTCTCTTTCAAAGACGAGGGTGTTGTTTTCTTTAGTAAAAATAT
    TGCCGCAGGAAAGGGGGGCGCTATTTATGCTAAGAAACTGACAATTTCTGACTGTGGTCCGGTCCAGTTT
    CTTGGTAATGTCGCGAATGACGGGGGCGCTATTTATCTAGTAGATCAGGGGGAACTTAGTCTATCTGCTG
    ATCGCGGAGATATTATTTTTGATGGAAATTTAAAGAGAATGGCTACGCAAGGCGCTGCCACCGTCCATGA
    TGTAATGGTTGCATCGAATGCTATCTCTATGGCTACAGGGGGGCAAATCACAACATTAAGGGCTAAGGAA
    GGTCGCCGAATTCTTTTTAATGACCCTATTGAAATGGCGAATGGACAACCTGTAATACAAACTCTTACAG
    TAAACGAGGGCGAAGGATATACGGGGGACATTGTTTTTGCTAAAGGTGATAATGTTTTGTACTCAAGTAT
    TGAGCTGAGTCAGGGAAGAATTATTCTCCGAGAGCAAACAAAATTATTGGTTAACTCCCTGACTCAGACT
    GGAGGGAGTGTACATATGGAAGGGGGGAGTACACTAGACTTTGCAGTAACAACGCCACCAGCTGCTAATT
    CGATGGCTCTTACTAATGTACACTTCTCCTTAGCTTCTTTACTAAAAAATAATGGGGTTACAAATCCTCC
    AACGAATCCTCCAGTACAGGTTTCTAGTCCAGCTGTAATTGGTAATACAGCTGCTGGTACTGTTACGATT
    TCTGGTCCGATCTTTTTTGAAGATTTAGATGAAACTGCTTACGATAATAATCAGTGGTTAGGTGCGGATC
    AAACTATTGATGTGCTGCAGTTGCATTTAGGAGCGAATCCTCCGGCTAACGCTCCAACTGATTTGACTTT
    AGGGAACGAAAGTTCTAAATATGGGTATCAAGGAAGTTGGACACTTCAATGGGAACCAGATCCTGCGAAT
    CCTCCACAGAACAATAGCTACATGTTGAAGGCAAGCTGGACTAAAACAGGTTATAATCCTGGTCCGGAGC
    GCGTAGCTTCTCTGGTCTCTAATAGTCTTTGGGGATCCATTTTAGATGTGCGTTCCGCGCATTCTGCGAT
    TCAAGCAAGTATAGATGGACGAGCTTATTGTCGGGGTATTTGGATTTCTGGGATTTCGAACTTTTTCTAT
    CATGATCAGGATGCTTTAGGACAGGGGTATCGTCATATTAGTGGGGGATATTCGATAGGAGCAAACTCTT
    ATTTCGGGTCTTCTATGTTTGGACTTGCTTTTACTGAAACTTTTGGTAGGTCCAAAGATTATGTGGTCTG
    TCGATCTAACGATCACACTTGTGTAGGCTCTGTTTACTTATCCACTAGACAAGCGTTATGCGGATCCTGT
    TTATTTGGAGATGCTTTTGTTCGGGCGAGTTACGGATTTGGAAATCAGCATATGAAGACCTCTTATACAT
    TTGCTGAAGAGAGTAATGTGCGTTGGGATAATAACTGTGTAGTGGGAGAAGTTGGAGCTGGGCTCCCTAT
    CATGCTCGCTGCATCTAAGCTTTATCTAAATGAGTTGCGTCCCTTCGTGCAAGCAGAGTTTGCTTATGCA
    GAGCATGAATCTTTTACAGAGAGAGGGGATCAGGCTAGGGAGTTTAAGAGTGGGCATCTTATGAATCTAT
    CTATTCCAGTTGGGGTGAAGTTTGATCGATGCTCTAGTAAACATCCTAACAAGTATAGTTTTATGGGAGC
    TTATATCTGTGATGCTTACCGGTCCATTTCTGGAACGGAGACAACACTCCTGTCTCATAAAGAGACTTGG
    ACAACAGATGCTTTCCATTTAGCAAGGCATGGAGTTATGGTCAGAGGATCTATGTATGCTTCTTTAACAG
    GTAATATAGAAGTCTATGGCCATGGAAAATATGAATACAGGGATGCCTCTCGAGGGTATGGTTTAAGTAT
    TGGAAGTAAAATCCGATTCTA
    
    
  • Protein Sequence : Show Sequence
    >WP_010229977.1 autotransporter domain-containing protein [Chlamydia muridarum]
    MQTPFHKFFLLAMLSYSLLQGGHAADISMPPGIYDGTTLTAPFPYTVIGDPRGTKVTSSGSLELKNLDNS
    IATLPLSCFGNLLGNFTIAGRGHSLVFENIRTSTNGAALSNHAPSGLFVIEAFDELSLLNCNSLVSVVPQ
    TGGTTTSVPSNGTIYSRTDLVLRDIKKVSFYSNLVSGDGGAIDAQSLMVNGIEKLCTFQENVAQSDGGAC
    QVTKTFSAVGNKVPLSFLGNVAGNKGGGVAAVKDGQGAGGATDLSVNFANNTAVEFEGNSARIGGGIYSD
    GNISFLGNAKTVFLSNVASPIYVDPAAAGGQPPADKDNYGDGGAIFCKNDTNIGEVSFKDEGVVFFSKNI
    AAGKGGAIYAKKLTISDCGPVQFLGNVANDGGAIYLVDQGELSLSADRGDIIFDGNLKRMATQGAATVHD
    VMVASNAISMATGGQITTLRAKEGRRILFNDPIEMANGQPVIQTLTVNEGEGYTGDIVFAKGDNVLYSSI
    ELSQGRIILREQTKLLVNSLTQTGGSVHMEGGSTLDFAVTTPPAANSMALTNVHFSLASLLKNNGVTNPP
    TNPPVQVSSPAVIGNTAAGTVTISGPIFFEDLDETAYDNNQWLGADQTIDVLQLHLGANPPANAPTDLTL
    GNESSKYGYQGSWTLQWEPDPANPPQNNSYMLKASWTKTGYNPGPERVASLVSNSLWGSILDVRSAHSAI
    QASIDGRAYCRGIWISGISNFFYHDQDALGQGYRHISGGYSIGANSYFGSSMFGLAFTETFGRSKDYVVC
    RSNDHTCVGSVYLSTRQALCGSCLFGDAFVRASYGFGNQHMKTSYTFAEESNVRWDNNCVVGEVGAGLPI
    MLAASKLYLNELRPFVQAEFAYAEHESFTERGDQAREFKSGHLMNLSIPVGVKFDRCSSKHPNKYSFMGA
    YICDAYRSISGTETTLLSHKETWTTDAFHLARHGVMVRGSMYASLTGNIEVYGHGKYEYRDASRGYGLSI
    GSKIRF
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. Overall, vaccination with DCs transfected with PmpG-1(25-500) exhibited the greatest degree of protective immunity among the four Chlamydia Ags tested (Yu et al., 2009).
  • Related Vaccine(s): C. muridarum PmpG-1 protein vaccine
7. pmpH
  • Gene Name : pmpH
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • NCBI Protein GI : Q9PL44
  • Other Database IDs : CDD:273587
    CDD:311484
    CDD:214872
  • Taxonomy ID : 243161
  • Protein Name : Probable outer membrane protein PmpH
  • Protein pI : 7.11
  • Protein Weight : 100297.73
  • Protein Length : 1134
  • Protein Note : Polymorphic membrane protein H
  • Protein Sequence : Show Sequence
    >sp|Q9PL44.2|PMPH_CHLMU RecName: Full=Probable outer membrane protein PmpH; AltName: Full=Polymorphic membrane protein H; Flags: Precursor
    MPFSLRSTSFCFLACLCSYSYGLASSPQVLTPNVIIPFKGDDIYLNGDCVFASIYAGAEQGSIISANGQN
    LTIVGQNHTLSFTDSQGPALQNCAFISAEEKISLRDFSSLLFSKNVSCGEKGMISGKTVSISGGDSIVFK
    DNSVGYSSLPSVGQTPTTPIVGDVLKGSIFCVETGLEISGVKKELVFDNTAGNFGAVFCSRAAQGDTTFT
    VKDCKGKILFQDNVGSCGGGVIYKGEVLFQDNEGEMLFRGNSAHDDLGILDANPQPPTEVGGGGGVICTP
    EKTVTFKGNKGPITFDYNFAKGRGGAIQSQTFSLVADSAVVFSNNTAEKGGGAIYALEVNVSTNGGSILF
    EGNRASEGGAICVSEPIAANNGGLTLHAADGDIIFSKNMTSDRPGERSAIRILDSGTNVSLNASGASKMI
    FYDPVVQNNPATPPTGTSGEIKINESGSGSVVFTAETLTPSEKLNVINATSNFPGNLTVSSGELVVTKGA
    TLTVGNITATSGRVTLGSGASLSAVAGTAGTCTVSKLGIDLESFLVPTYETAKLGADTTVAVNNNPTLDL
    VMANETEMYDNPLFMNAVTIPFVTLVSLQTTGGVTTSAVTLNNADTAHYGYQGSWSADWRRPPLAPDPSG
    MTPLDKSNTLYVTWRPSSNYGVYKLDPQRRGELVPNSLWVSGSALRTFTNGLKEHYVSRDVGFIASVQAL
    GDYVLNYKQGNRDGFLARYGGFQAVAASHYENGGIFGVAFGQLYGQTKSRLYDSKDAGNITILSCFGRSY
    IDVKGTETVVYWETAYGYSVHRMHTQYFNGKTNKFDHSKCRWHNNSYYAFVGAEHNFLEYCIPTRQLARD
    YDLTGFMRFEMSGGWSSGAKETGALPRHFDRGTGHNMSLPIGVVAHAVSNGRRSPPSKLTINMGYRPDIW
    RVTPHCNMKIIANGVKTPIQGSPLARHAFFLEVHDTLYVRHLGRAYMNYSLDARHRQTTHFVSLGLNRIF
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Yu et al., 2014)
8. RplF
  • Gene Name : RplF
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • VO ID : VO_0010892
  • NCBI Gene ID : 1246168
  • NCBI Protein GI : 497917443
  • Locus Tag : TC_RS04070
  • Genbank Accession : AE002160
  • Protein Accession : WP_010231599
  • Taxonomy ID : 243161
  • Gene Starting Position : 941594
  • Gene Ending Position : 942145
  • Gene Strand (Orientation) : -
  • Protein Name : 50S ribosomal protein L6
  • Protein pI : 10.64
  • Protein Weight : 18060.75
  • Protein Length : 183
  • Protein Note : ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance
  • DNA Sequence : Show Sequence
    >NC_002620.2:941594-942145 Chlamydia muridarum Nigg, complete genome
    TCTATTTTTTACCAGTTTTCGCTGCTTTTCCAGCCTTACGACGTACATATTCGTTTTCGTAACGAATTCC
    TTTACCTTTGTATGGCTCTGGAGGGCGTTTTGCACGAACACAAGCCGCAAATTCTCCTACCAACTGCTTG
    TTGATACCTTTGATGGAGATCAATGTGTTCTTCTCAACAGAGACTTCCAATCCTGTAGGAATAGGCATTT
    TTGTTGGATGGGAAACCCCTATCGATAAATCTAAGAAGGACCCCTGTACCGAAGCTCTGAATCCAACTCC
    GATCATTTCTAAACGTTTTTCAAATCCAAGGTGGACACCTTTGACCATATTTGCTATCAAAGCCCAATAA
    AGCCCTTGCATACGACCAGGTCTGTCTATAATGTGAGCTGCGGGAGAAACAAACACCTCATTACCTTTAA
    CGGCAATCTCAACTTCTTTAGCCAACACCTGTGTCAAAGACCCTTTAGGACCTTTTACTGAGATTTCATC
    ATTTTGAATAGAGACCTCTACGCCTTGAGGAAGTACAATAGGGTCTCGAGCTTTACGAGACA
    
    
  • Protein Sequence : Show Sequence
    >WP_010231599.1 50S ribosomal protein L6 [Chlamydia muridarum]
    MSRKARDPIVLPQGVEVSIQNDEISVKGPKGSLTQVLAKEVEIAVKGNEVFVSPAAHIIDRPGRMQGLYW
    ALIANMVKGVHLGFEKRLEMIGVGFRASVQGSFLDLSIGVSHPTKMPIPTGLEVSVEKNTLISIKGINKQ
    LVGEFAACVRAKRPPEPYKGKGIRYENEYVRRKAGKAAKTGKK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. In addition, RplF was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
  • Related Vaccine(s): C. muridarum RplF protein vaccine
9. Tarp
  • Gene Name : Tarp
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • NCBI Gene ID : 1245826
  • NCBI Protein GI : 497916366
  • Locus Tag : TC_RS02355
  • Genbank Accession : AE002160
  • Protein Accession : WP_010230522
  • Taxonomy ID : 243161
  • Gene Starting Position : 571045
  • Gene Ending Position : 572607
  • Gene Strand (Orientation) : +
  • Protein Name : peptide ABC transporter substrate-binding protein
  • Protein pI : 9.01
  • Protein Weight : 56833.03
  • Protein Length : 520
  • Protein Note : similar to GB:D00760, SP:P25787, and PID:220024; identified by sequence similarity; putative
  • DNA Sequence : Show Sequence
    >NC_002620.2:571045-572607 Chlamydia muridarum Nigg, complete genome
    CATGCGCAAGGTATCAGTGGGAATCTGCTTGCTTATCGCATTAGCAACAGCAATTACCGGCTGCTCCAAA
    TCCTCCTCAAACAAATCAAATCACTCCTCCTCTAATCAAAGCGTATCTGTGAGCATGAAGGACGATCCTC
    GCACGTTTGACCCTCGAGAGGTTAGGCTTCTTTCGGATATTAATTTGATTCACCATCTTTATGAGGGACT
    GGTTCAAGAGACTCCTTCCGGAGAGGTTTTCCCTGCATTAGCAGAAAGTTTCTTCCTATCTGAAGATAAG
    AAAACCTACACTTTCCACCTAAAAAAAGCACTCTGGAGTAATGGAGACCTGATTACTGCTCATGATTTTG
    TACGTTCTTGGAACGATGTTTTGCAAAATCGCATTGCAAGTATCTACTCTTTCGCTTTTTTACCCATTGA
    TTTAAGTAAAGATTGTGGATTCTTCGCTAAGGACAATCATACACTTGTTATCAATTTGCATACCCCTACT
    CCACACTTTCTGAAATTACTTACCCTTCCAGTATTTTATCCTGTGCACCCCGAACATCAAATTCGGAATG
    AAGCAAAAGCTCTTCCAATATCTACAGGGGCTTTTTGTTTAAAAGAGAAAAAAGATCGGCGGTGGCTTAA
    ATTAGAAAAAAATCCCTATTATTATAACAAAGAGCAAGTTGCGATCCAGGAAATCCACATACATGTGATT
    CCTGATCAGCAGACCGCTTCTGCTTTGTTCAAACAAGGAAAATTGGATTGGCAAGGACTTCCTTGGGGAC
    ACAGCATTCCCCAAGAAGCTTTAGCAACAGCAAATAAACGTCGTACTCCTCAATCATTTGATATATCTGG
    CACCTCGTGGCTCACGTTCAACACATCAAAAGTACCCTTCAGCCATCCCAAGTTGCGACAAGCTTTAAGC
    CTTGTTCTAAATAAGGAAGCCTTAGCCTCTCCAACCTTTGTTAAGCCCGCTAAACACCTACTTCCTACAC
    ACCTGCACACTTATCCAGAGCAACCTACTTACAAACAACAAGAAGCCGTCATTTTAGCCAAAACTCTGTT
    ACAAGAAGCATTAACAGATCTTAATATGACTATAAAAGATCTTGAAAAATGCCCTTTGATTTTTTCAGCG
    ACATCTAGCGTCAACTCACAAATGGCCCAAATGATGCGCGATCAATGGCGAAGAATTTTAGGAATAACAT
    TCCCTATTTGCGGGAAAGAGTATGCTTTATTACAAAATGATTTGACCACCAACACTTTTTTTATGTCCAT
    AAACGGTTGGTTTGCAGACTTTTCCGATCCTTTAGCCTTCTTGTCTGTTTTTTCTTCTAAGGGAATCAAA
    CCTTATGCCTTACAAGACCCTCTGTTTGACCAGCTTATTCTCTCTATAGAAACGGAAAAAGATCCTCGAA
    AGCGTATTTCTCTGATTTCCGAAGCTTCTCTATACCTAGAAAAACAAAATATCATAGAACCGCTCTATCA
    CGATGTTTTTCACTATGCCGCAAATAATAAACTTTCTTTTGTTAGATTACATCCCTCAGGTCTTGTCGAT
    ATGCGGTATGCAAAAAACTCTTG
    
    
  • Protein Sequence : Show Sequence
    >WP_010230522.1 peptide ABC transporter substrate-binding protein [Chlamydia muridarum]
    MRKVSVGICLLIALATAITGCSKSSSNKSNHSSSNQSVSVSMKDDPRTFDPREVRLLSDINLIHHLYEGL
    VQETPSGEVFPALAESFFLSEDKKTYTFHLKKALWSNGDLITAHDFVRSWNDVLQNRIASIYSFAFLPID
    LSKDCGFFAKDNHTLVINLHTPTPHFLKLLTLPVFYPVHPEHQIRNEAKALPISTGAFCLKEKKDRRWLK
    LEKNPYYYNKEQVAIQEIHIHVIPDQQTASALFKQGKLDWQGLPWGHSIPQEALATANKRRTPQSFDISG
    TSWLTFNTSKVPFSHPKLRQALSLVLNKEALASPTFVKPAKHLLPTHLHTYPEQPTYKQQEAVILAKTLL
    QEALTDLNMTIKDLEKCPLIFSATSSVNSQMAQMMRDQWRRILGITFPICGKEYALLQNDLTTNTFFMSI
    NGWFADFSDPLAFLSVFSSKGIKPYALQDPLFDQLILSIETEKDPRKRISLISEASLYLEKQNIIEPLYH
    DVFHYAANNKLSFVRLHPSGLVDMRYAKNS
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Immunization of mice with Tarp induced Th1-dominant immunity that significantly reduced the shedding of live organisms from the lower genital tract and attenuated inflammatory pathologies in the fallopian tube tissues. Tarp, an immunodominant antigen identified by human antisera, can induce protective immunity against chlamydial infection and pathology in mice (Wang et al., 2009).
  • Related Vaccine(s): C. muridarum Tarp subunit vaccine
10. TC0439 adherence factor
  • Gene Name : TC0439 adherence factor
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • VO ID : VO_0010887
  • NCBI Gene ID : 1245792
  • NCBI Protein GI : 497916297
  • Locus Tag : TC_RS02190
  • Genbank Accession : AE002160
  • Protein Accession : WP_010230453
  • Taxonomy ID : 243161
  • Gene Starting Position : 526719
  • Gene Ending Position : 536375
  • Gene Strand (Orientation) : +
  • Protein Name : adherence factor
  • Protein pI : 7.34
  • Protein Weight : 344190.12
  • Protein Length : 3218
  • Protein Note : similar to GP:6013469; identified by sequence similarity; putative
  • DNA Sequence : Show Sequence
    >NC_002620.2:526719-536375 Chlamydia muridarum Nigg, complete genome
    TATGACTCTTTCGAATACAGCCTCTTCCACAGTTTCACCTAAGTCTACGCAACTTAACCATACTTTATCC
    AATCAAACTCACCCTGTTGCTCAAAGCAATAGGAATATAGATTTCCAAATCTATGATGAACAGATTCATA
    ATAATCATTCTACAGAAGATGTAGTTGCTATAGGACGTCGTATTCAGCAAGAATATGCGAATCTTACCAC
    ATCAAAACAGGTTAATTTCTCGGCATCTCCATCTAACCATACTGGAAACTGGAAGATTTCTTTGCTTTAT
    AATTTATCGATGCTGGTTGCAAATCTATTCCCTACAACTATTCAGCCTGTGCAACCTCAAAAAGTCATCT
    TTAAGAATCAAGACTCTTCTAAGGAAACCCAACTGACCAAATCATCCAAAGTAGTTTCTTCTCGCTCTTC
    TTTAAAAGAGATTTTTTCGAAGAAATCTCCTCCTTTGAGAAAGTCTTTTTCTTCCTCTAAAATTTCTAGA
    GCAATCCCTAGAAGAAAAAAACGATCAAACGATGATCCAGTTCAAGCCACAAATACTTATGATTTGACCG
    CTGAAAATATTCTTGAAAAACTCTCTTTAACACAAGAACAACAAATTAAGCACGATAATCTGATTAGCAA
    TCTCAAAGAAGCAATTAATCGATATAGTGATCTTAATCGGAAAAATTCCCGGAAAGGACAATCCCTTCTT
    GTTAGGCAAGCCAAAATTCTAGATGAAATTCTTTCACAGACTAAATCTACAGAGGAACGAGCTTCCAATT
    CTGTAATGACGACTATTAAGAAGGAATTTACCTCCCACCGCGTACCTGTAGAGAAAAATATTCACGGAAT
    TTGGATTGCAGGTTCACCGCCAGAAGGGACCGATGAATATATTAAATTGTTCCTTCACACTTATCCAGAG
    TTTTCCTTCCTTTTTTGGGTGGATAAAACAGCTTATGGCGCAGCAAAATTCTCTTCTACTCTTAAACGAA
    TTGCTTTTGATGCAGCAGTTAATTCCTTACGAGAGGCTACTCCAGAGCCTGTCAAGCAGTTTGTTCAACG
    TTATGATAAGCTAAAAAAATCATACGATACTTCGCGAGATTTTGATGAAAAACAGCGGTTATCTGAGCAG
    TTAGTTGAGCTATACGATAATTACAATAAATTTAGTAAGGAGATTCAAAGTAACTTTGATGTTTTGCTAT
    TGCATGAAATGATCACTATTCAAGATAGCTTTTTTAACTACTGCCAGCTCAAAGGAGTAGGTGCAATAAC
    AGATGAGACACGTATAGAGTATCTTGAGAAAGTTTTGAAAGTAAAAGAAGAGGATCTATCTCACTATAAA
    GAAACTATCAAACGCAACAAAGAGTCTATTGAAAAACTTGTAAAAGAGATTAATGACTCTACTGGACGGG
    AGAGAGTAGTTATCAAAGACATCCGAGATTTGAAGTCTTTACAAGATCTTACTAATAGCTACAACTATGA
    AACAGAAATGTTACTCCGATGGAATTATGCAGCGGCTACCGACCAGCTTCGTATGTACATGCTTAAAGAA
    TATGGGGGGATTTATACAGACCTAGATATTATGCCACAATACTCTCAGGATGTTTTGCAAAAAATTATGG
    ATGTTGGTGGGAGTAGGTTTTTTGAGCATGATAAGTTACGGCGGACTCTTTCTTTTGCAGCTCTAAAATT
    AGGAAGCGGTAAGCAAACAACTGTTTCCTTTGAAGAGGCTAAAAAAGCTATGACTCTCCCCACATTTACT
    CTTCAGGATAAGAGTCAAATTTCTGAAATTTTTAAATATCTAGAAACGGAAACGCAAGCAAAAAAATCAC
    TTTTCCAACCGATGGATGTTACCGTCGTCCGTGATTTCATGCCTATTTTACAACGTTATCATAAGTGGCA
    AACAGGATGGAATGTTCGGGGATTAAATGGTTTAATGATGGCTCATAAAGATAGTGCTGTTGTCGATGCT
    GTTATTGCTCGCCAGCGAGCGGCCTATGATGAGATGCGTGCACTTCGTCAGAATGTTGTTAGTGGAGAAT
    TTTTTCGTTCTCTTGGAGATCTTGAACATGTAAATCGTGAAAAGAATATCGGCGGCTATTTAGCGAAAAA
    TTATCTTGGGGGCAGTCTTTTTTTCGATTTTCGTCAAGATTCAGTGATTCCAGGAGCTATTAGTACCTTA
    GGAATTTCAGGTCCCGATATCATCATGGATACCATGAGCGATTATTTTACAAATCTTGGACCAGTTGGAG
    AAGATTTTTTATATGAAGGGAAATTAGGAAAAGCAGCTTTCCTTGGAGCTTATCAAGCACAAAAAACTCC
    GAAAGGAGAACTTACCTATGATTGGCTCCATCCTTTATCCATTGGAGCTAATGACGTTACTCCTGCTGAT
    GCAAGCACCTGGTGTGAAACTCGACAGCATTGTGCCGCTGAGCTTCTTTTATCGGACTCTATATCCTCAG
    ACGAACACCCTAAGGGTATTCGTCGAGAAAGAGTAAACCCGAATGATTTCTCTAAATTATGGAGTAAGGA
    AGCTCAGGGAATCCTTTCTAGTGATTTCGCGGATCTTTTACCACGCTTTAACTTACTGATAGAATCATCT
    GCGTTAGATATTCATACTTTATCGGCTCTTGATCGAGATATCCAACACCTTTTTACAAAAGTTCAAAAAG
    ACCCTGTAGCATCTGTAGCTGTATTTTCTTTACAGTTACAATTAGCTGAAATGATTCGTGCGATTCCTTT
    CCCTATACGTAATCAGGTACATATCTTGCCTGAAGCACAAGCACATTTCGAAGCAGATTGGAAGAAAGCA
    ATCCAATTATATCTTCACAGTCATCCCCAAACAGAAGTGGTTATTTGGTACAGTTCTACTCATACTCAAA
    TTGTTTTTGGAAAAGATTTGCTTGCTGTTGCAGAACGAGTTGCAGCAGCAAAATCCTTAATGTCTGATCA
    TGATCCATCACTTATAACCAGTTATCTAAAATATAAAACTCAATCTCATCTTGGGGTGCTTACTGAGTTT
    GATCAAGAAGATTTCTTTGAACTGATGGTAGATATTGCAGAGGAACCAGAGCTTCATAAACAACTATTGA
    AAATAGAAGAACAAGTTAATTCAGGACTCTACTCTCATGTAGAGCATTCTTTAGGAGAGTGGTTAAAATT
    ATCCAAAGAAGAAAGAAAATCAAAGTTTCTTAAGATTTTAAAAGAAACGTTTCAAGAGGAGGAAAGAGAA
    GACTCTCAACAACAACATAAAACATGGTTTGAAGAACTCTATGAGAAAAGACATCAGGAGCGCGTAAAGG
    ATCCAGCAAAAAAAATCCAAGAACTAATCACAGTTTTTCAAGAAAGTCAGCGCGTTCAAGCTCAAGACAT
    AGATACTTATTTTGCTCATAAACCGTTTTATCAAGATCTGATGAAAGATGGTTACGCTTTTGAGGACATT
    TCTGTGATTACGAAGTATCTTTTAGCGAGTGACGGTGTTTCAGGGATTATTACTACTGATCCTATATTTC
    CTCCTCCATCTAAACAGCTAATTGACGCTATGAAACAATCTTTGGGAGAAGATTTTGGTGAGCTACACTA
    TACGTTACAGATGGTCTATGATTGGTTATCTAAAGAGACTAACAGCGTAACTTCTGAACAGGCAAAACAA
    AAATTACCACAGAAACTACATGAAAAACTAGAAGGTTATACAACACATGATTTGCTTATTCCGCCTATTG
    ATGGAAGCGTCAGCGCTTTAGGATTGCGATTTTCCACGGAGGAGGGGAAGGTATCCGATCGCGTTTTAAC
    AAGCATAGCACCTGGAGTTCCTAATTCTGCTAGCTATGCTATGACTAGTTATTTGTATGGTTTGTTCTTA
    ATTACGAAAGATATCCAAAGTGGTCGATTAACCCATGAAATACTAAAAGAGAGATTACAAACCTATGGAG
    GCGCCTATTTTATCAATGAATCAAAAATTGATGTGCTACTTGCTTTATCCCGGAAAAAAGCTCAGATATC
    CCTAATAGACGCCCATAAGGCTCTTACAGGTTTTTCTTCTTTTAGCGAAGCATCTCTGGCTTTACTTACA
    GGAAGGATGCCCGGAACAAGCAGAGTTCTATCAAGAGAAGTAGAGTTTGGACGACCTTCAGCTATCGTTA
    TGGAAGGAGCAACAGCTATTCGTGCTCAGAGTTACGATGCTGTTGGATTAAGGAAAGATTTTTTCTTGTT
    ACCTCATACAGTTCCTTCCATACAGTCTATTGTTGAGCAAGCGAAATATACAGTATTATCCTGGCCAGAA
    TTTTATGAAAATCATGCGGATAAATGGAACGATCTTGCTAATCGTTTTGGAGCTGAGGACCTTAGTGTTC
    ATCCGCAAACATTTTTATATGACACAGAAGGTCGCTGTATGGGGCTTGCTTTACTGTATATGTTAGCAGA
    TGACTCTGTATCCTACAGATTATTACAACAAAATCTGATGACTTTAGCTTCGTTATTTGATGAGCAAAAC
    AGAAGAAATATCCCTTTAACTCCAGCTGATCAGAAATTTTTAAATAAGGGGCTTTCTTTAATTGAGTGGT
    TACAGTTCAAAGGAAATCAACAACTTCAAACAGAGGGATTTTTCCATACCTTAGACTGGGATATCCCTCA
    ATTAATGAAGCACTTTGCTTCTTCAACTGTAAAAAGTTGGTTAATTACTACGCCTGCGCATAGTCTTGTT
    CTTTCTTTAATGGGGAATTTTTTTAGAGTAACAGATCCAAACTATGGCCATACAGACTTTCCTTCATTAG
    AAGCTGCTATTACTTTTTTAGAAAGGATGGTTCAAGTATCCCCAGCTGTTTTGGAGCGTTATGGATTTGA
    TAAGGAAAAGTCTGTAACTAGCCAACTCAAAGTCCACTCTTTAGAGACTTCAGAATTGCAAAATGCAGTT
    TTTGCTTCCTCAGATCTTGGCTTTACTAGTCGCTATTTTACGACAACGTTAGAAGAGATGACCGTCAGAG
    GCCCTATTACGATGAATCAACGTCACACGGATTGGGCCACTCTTTATAAGATTGGGGGGACTGTTCAAGG
    TAAACGTATTGATAGTAGAACACGGGAATCGGATTTAAATTTTTTAAAAATTAATGGGGATATTCTAGAG
    GAATTTTTAACAAGAACTGTTCTAGATAGTGACTTGGTGGAGTTAATACAATCTCTGCTCAAAACACATG
    GCCTTGAGCCAGGCACTACCCTTATTAGTCCAAGTTCTATTGTAGAGACCGCAATAGACCACGTCTCGCT
    ATTACAAGCGGTGAAAACAAAAACCTCTCGAATGCACACTATTTTACAAAGCCTAGGAGAAAGAATTTTT
    AAATTGTTTAAAAATTCTGGGGTACAAGACTCAGACAAAATTTCTATAGACAGAGTCCAACTAGTTGATG
    AATCTGATAGCGCAACGATAGACTTCACAGTGATTAAAGATAAACAGCGTTCTCAGAAAAAAAGTATTAC
    TGTTGGCATAGAAAGTTTAGCTGGATCCTTTAGAAAATTTAGCGCATCGATGCACGAAGTTATTGGAACT
    GGAGTGCTTGATTTAGACTTAGGAATGACTGTCGTCTCTTTAGTTCAGTATGTCCGTTTAGTAGAAGCAG
    GACAAGGAAAAGATGCTTTAGCAGTTGCTAATTTAGTGATGAATCTTAAAATAGCTCTCGAGGTCTCTAT
    AGGGAATGTAATTCAGGCTCTGGGTAAAAAACTGCTCACTCAGGAGGGGCTTAATACTTTCCGTTTAGAA
    ACAGAATTAGCTCGTCAACTACACAAAGTGGGAGCACGAGTTGGAGGAACTGTTGGTAAGACGTTAACCA
    GAGTAGCGCATGTATTAGAACTCCCCATATTAGAAAGTGCAATTGGAACATGGAACCTCTATAATAGTGT
    GAACGAGTTATTACATGCTGATTCTTGGAGTGACCAGGTTGCTGCTAGAGTACAAGTAGCTTTTGATTCA
    ATCTCTTTAGGAATCACTATAGCTTCGGTTGTTTCTCCAGCACTTATGATGGCAGCAGGCCCCATTGCTG
    CCATAGGGATGGGGGCAGCATCCATTGCTCGTAATGTTGCCAGAAAAGAAGAGCGGCACTCTCAATGGCT
    CAAGTACAAGAGTTTTCTTGATAATGGATCAAAACATACAGTTGCCGCATTCCCTCATAAAGGGCTTATA
    GATTTATCTGAGAATTTAGTACTTGGGAACATTGTGCTGAATTTAAGGGTTTACCCACCTCTGCTTACAG
    GCGATCGCTCTTATAATGCAAATCGGTGGTTTGGGAATAAGCCTGGATGGTCTGATTGGCAAGTTCGAGA
    AAGATTAGGTTATGCCTATCGAAGCAGTCCATCTTATGCGTTAGCCAGAGGACACGCTAATAGTTTTTGG
    CCACTATCTATGCCTAGTATTGATAAAGGTGTATATCGCACAGTAATTCTAGGATATGGCATTCAATATA
    AAGCGGTTACAGAAGTTGTCTACTTGTCTAATGAAATGGTTTGGAGAGAAGCTGTGATGCAGTTTGACTC
    TCGATACTATGTAGAGCCATTAACAGCGGAAAAGGAGTGTGCAACAGTACTAGCAGGAGATACGCCACTA
    TCAGTGATTCCTGTACGTTTATTAGAAGAGGAGTCTCTGGAACGCGAGAAGAACGCGGCTTCTTATAAGA
    ACTATCAGATCATTATTGAGGGAGGGAAAGGAGGACTTACTGTACAGATAGGAGGAGCAGGTTATTACAA
    GTTAACAGCACAGCCAGGAAAAGGGAATATCCTCTCTTTCCGAGCAATACCTGGATATCTTTCTGTAACG
    TTTGATCTTAGCCGATTGGAACAAGAGGTGCCTCTCACCAAACAAAATGGAACAGCCTTAAAGATACTTA
    AAGTACGTCAAACTGGGTTTGATACTATTGTTGGATCTTCAGGAGGTAGTGATAATTTAACAGGCAATCA
    CAATACAAAATTTTATCTAAGCACCGGAGGAGGACATGTCACCTCAGGATCCGGTAAGAACTGGTATAAC
    ATTCCATCTTTAACACGTAGCCTTGGTTTTGATTTTACTTCTAATGCTATAGAGCATAAGGCTTCTGTAG
    AGATGCTTTTAGCAGATTTCTCTCCTGCTAATAATTTAAGTTTAATAGTTGAACGATTTGCAAATGTTAG
    CATTCATGTTTTTAATTATAGCAATAAAACAGCCCCTTATACTGTGAATTTGAAAGACGGGGTGTCTTTA
    CAAGCTTCTAAGCAAGGCTTTAGCGGTAGTTTATTAGAGGTGGCTTCCTTTGATCAAACGCTATGGCAAA
    AGAGCTATCCAGAAGATAGAGGATTTGTAGAAGATATTTTATCTTGGTTATTAAAACTGCAATGGTCGTT
    AGCTTCTCGAGTAAGGATCTTTTTACAGGGAGGCACAGCACAATACGAAACAACGCAGAAAAGTCTTATT
    TATAGACCTGATCCACACTCAAGTATATCTATTCAGGCTACAGATTCTTATAATACACAAGTTTATGGTA
    ACGTTGGCTGTTCTTATATTCTATTCTCTTCACCAGGTGTGACCGCCAAAACACTCGATATTTTGTTATT
    CGAAGATTCGGGGTTACCACAAATTATTGACTTAAGCACACTTGTTCCAACTTCTATCCAAGGGTTCCTC
    TATCCAGGAGGGTTTATAAACTTTCAAGTCTCTTCTGCAAGATATGCATTTCCTTTTTCTGTCAGTTGGA
    GAAGGCATCATTACACTTTCCCTGCTCAAACGATTATTCAAGTTCTACCTCGCTTGCGGTTTGAACTAGG
    GGATTGGTTTACAATTTTGCAAAAGAGTGTAGGAAAATGGGTAACATTGTACCAACACGACATGATTATC
    CCTGAACGTATTGAAGGTGTTTTAAGTCTAAATAACACAGCGACATTAATGTCACACCACAATCAAACAA
    CCCATATGCTTGGAGTAGAAAACAGGGGAGATTTGAGTCTAAAAGTTTTAGGAATACTCCAATCTGGTCA
    AATTAAAGGATCTAAATCGGAAAATAGCGCGAGTGGTCATTTTTCTAAATTGTTATCTGTCCCAGCACAT
    ACGATCAAAAATCTTTCGTTTAAAGAGGAAGAGGGAACTAAGAGTAAAAATATTTTATTCTACAGTGTGC
    TGGAGGAAACATTCCTTAAAGCTACAGATAAACCTTTAACTATTATTAAACGTGATAAATGGTCTCTGTA
    TGATGAAATCCAAGTATTTGCAACAACTCTTAATCTGCAAAATTTCTTGCGTTATCATATTTCGGAAGAG
    ACTCCAATGTTATCTAGACTGCTCATGTATGCGCAGAAACGCGTTTCCATCCAAAATAGAGATCTCGCAT
    TGAAATTTTTCTATGTTCGAGAGCGAAGTGGTATTGGTGCAATTAGATTGGTATTCAAGAACTTTTTCGA
    AGAGAGCTTGCAGGGGATATTACATGGAACCTTAGAGCGAGAAGTTAAACCTATGCTAGCTGAAAATCCA
    AATACACTAATTCATTCATCGTATCGGAATCATTTAGAACTCATCCTAGGGGAGGAAACCTTAGATCTCG
    CAATTATCGTTCAAGAGTTTAGCTCTTCAAAAAATATTGTTGAGTTGCAAAAAGATCTAGCAACCCATCG
    GCTTATTTATCCTCAAGGAAAAGAAGCCTTGTCTCTTGCAATCTATACCCATACTTTCAGTAAAGAGAGC
    TTAGTCACATCTAAACAACGAAGCGAAGGGTTAAAATTCATCGATCCATCAATGCAGGAATATCGTTTAC
    CTGAGACAACGATTCTCGAAAACTCTTATTATTTAGACCCTTCCACAGGAGATTTGTATTTAACAAAGAT
    AATTGCGCAACCTTCACAAAGTAGAGCATTTCTTATCAAATTTAAAAAATACAAACGCCACTGGCTAGAT
    TTCCAAAAATTAGTTCTTTCTGGATCTCATTTAGAACTTATCGCATCTACTGGAACAGCTTTAACATTTG
    TAGGCCCCGAATTACGGCATTTAGAGATTGACTTCCCAGGCGCGTTAAATGGGACTATTGCCAAAGAACA
    CATCTCATCTCGGGCAAGTATTGTAATCCCAACAAACAATCAGGTGACACACTATGACCCAAGAATAGCG
    AAGCAGCATTATAGCTCTATCAATTATATGCTGTGGGATCTGCGTGATCGTGCGACACTATCTAAGCGAG
    CAAAAACTTTAGATAGCTATTTGTTAGAAGTTTGTATGAACTTAGACTCTTCTAATCCAACGTGGAATAT
    TCCAAATGATGTGTTGAACTACGCGGTAGGATATTATCGAACAATCCTTCCTAGCTGGGTAAAAAATAAA
    ATCCGTGTAGGTTCCTTAATTAAAGTAGAAGCTGATACTGCAAAAACTTTGAGTTTCTCACTAATTACGA
    CACAAAATGATCTATTCCAACCTGTAGTGGATAAAGGATTCTATATTTACTACAGCGTTTCGAAACTAGC
    CAATCATGTTAAACTGCGAAATGTTACTGGAGATATGTTGCTTGATATCGATAAGGAAACCACATTTATT
    GTTCGAGGAGTAGATGAATCTGATTATGATAAGAAACAAATCTATGTTGTTTTAGATCTGACAACAGAAG
    AAGAACGTAAATTGCGAACGGATAAGAACATCATTATTATTCCTGGAGGAGAACGATTGAACCGATG
    
    
  • Protein Sequence : Show Sequence
    >WP_010230453.1 LifA/Efa1-related large cytotoxin [Chlamydia muridarum]
    MTLSNTASSTVSPKSTQLNHTLSNQTHPVAQSNRNIDFQIYDEQIHNNHSTEDVVAIGRRIQQEYANLTT
    SKQVNFSASPSNHTGNWKISLLYNLSMLVANLFPTTIQPVQPQKVIFKNQDSSKETQLTKSSKVVSSRSS
    LKEIFSKKSPPLRKSFSSSKISRAIPRRKKRSNDDPVQATNTYDLTAENILEKLSLTQEQQIKHDNLISN
    LKEAINRYSDLNRKNSRKGQSLLVRQAKILDEILSQTKSTEERASNSVMTTIKKEFTSHRVPVEKNIHGI
    WIAGSPPEGTDEYIKLFLHTYPEFSFLFWVDKTAYGAAKFSSTLKRIAFDAAVNSLREATPEPVKQFVQR
    YDKLKKSYDTSRDFDEKQRLSEQLVELYDNYNKFSKEIQSNFDVLLLHEMITIQDSFFNYCQLKGVGAIT
    DETRIEYLEKVLKVKEEDLSHYKETIKRNKESIEKLVKEINDSTGRERVVIKDIRDLKSLQDLTNSYNYE
    TEMLLRWNYAAATDQLRMYMLKEYGGIYTDLDIMPQYSQDVLQKIMDVGGSRFFEHDKLRRTLSFAALKL
    GSGKQTTVSFEEAKKAMTLPTFTLQDKSQISEIFKYLETETQAKKSLFQPMDVTVVRDFMPILQRYHKWQ
    TGWNVRGLNGLMMAHKDSAVVDAVIARQRAAYDEMRALRQNVVSGEFFRSLGDLEHVNREKNIGGYLAKN
    YLGGSLFFDFRQDSVIPGAISTLGISGPDIIMDTMSDYFTNLGPVGEDFLYEGKLGKAAFLGAYQAQKTP
    KGELTYDWLHPLSIGANDVTPADASTWCETRQHCAAELLLSDSISSDEHPKGIRRERVNPNDFSKLWSKE
    AQGILSSDFADLLPRFNLLIESSALDIHTLSALDRDIQHLFTKVQKDPVASVAVFSLQLQLAEMIRAIPF
    PIRNQVHILPEAQAHFEADWKKAIQLYLHSHPQTEVVIWYSSTHTQIVFGKDLLAVAERVAAAKSLMSDH
    DPSLITSYLKYKTQSHLGVLTEFDQEDFFELMVDIAEEPELHKQLLKIEEQVNSGLYSHVEHSLGEWLKL
    SKEERKSKFLKILKETFQEEEREDSQQQHKTWFEELYEKRHQERVKDPAKKIQELITVFQESQRVQAQDI
    DTYFAHKPFYQDLMKDGYAFEDISVITKYLLASDGVSGIITTDPIFPPPSKQLIDAMKQSLGEDFGELHY
    TLQMVYDWLSKETNSVTSEQAKQKLPQKLHEKLEGYTTHDLLIPPIDGSVSALGLRFSTEEGKVSDRVLT
    SIAPGVPNSASYAMTSYLYGLFLITKDIQSGRLTHEILKERLQTYGGAYFINESKIDVLLALSRKKAQIS
    LIDAHKALTGFSSFSEASLALLTGRMPGTSRVLSREVEFGRPSAIVMEGATAIRAQSYDAVGLRKDFFLL
    PHTVPSIQSIVEQAKYTVLSWPEFYENHADKWNDLANRFGAEDLSVHPQTFLYDTEGRCMGLALLYMLAD
    DSVSYRLLQQNLMTLASLFDEQNRRNIPLTPADQKFLNKGLSLIEWLQFKGNQQLQTEGFFHTLDWDIPQ
    LMKHFASSTVKSWLITTPAHSLVLSLMGNFFRVTDPNYGHTDFPSLEAAITFLERMVQVSPAVLERYGFD
    KEKSVTSQLKVHSLETSELQNAVFASSDLGFTSRYFTTTLEEMTVRGPITMNQRHTDWATLYKIGGTVQG
    KRIDSRTRESDLNFLKINGDILEEFLTRTVLDSDLVELIQSLLKTHGLEPGTTLISPSSIVETAIDHVSL
    LQAVKTKTSRMHTILQSLGERIFKLFKNSGVQDSDKISIDRVQLVDESDSATIDFTVIKDKQRSQKKSIT
    VGIESLAGSFRKFSASMHEVIGTGVLDLDLGMTVVSLVQYVRLVEAGQGKDALAVANLVMNLKIALEVSI
    GNVIQALGKKLLTQEGLNTFRLETELARQLHKVGARVGGTVGKTLTRVAHVLELPILESAIGTWNLYNSV
    NELLHADSWSDQVAARVQVAFDSISLGITIASVVSPALMMAAGPIAAIGMGAASIARNVARKEERHSQWL
    KYKSFLDNGSKHTVAAFPHKGLIDLSENLVLGNIVLNLRVYPPLLTGDRSYNANRWFGNKPGWSDWQVRE
    RLGYAYRSSPSYALARGHANSFWPLSMPSIDKGVYRTVILGYGIQYKAVTEVVYLSNEMVWREAVMQFDS
    RYYVEPLTAEKECATVLAGDTPLSVIPVRLLEEESLEREKNAASYKNYQIIIEGGKGGLTVQIGGAGYYK
    LTAQPGKGNILSFRAIPGYLSVTFDLSRLEQEVPLTKQNGTALKILKVRQTGFDTIVGSSGGSDNLTGNH
    NTKFYLSTGGGHVTSGSGKNWYNIPSLTRSLGFDFTSNAIEHKASVEMLLADFSPANNLSLIVERFANVS
    IHVFNYSNKTAPYTVNLKDGVSLQASKQGFSGSLLEVASFDQTLWQKSYPEDRGFVEDILSWLLKLQWSL
    ASRVRIFLQGGTAQYETTQKSLIYRPDPHSSISIQATDSYNTQVYGNVGCSYILFSSPGVTAKTLDILLF
    EDSGLPQIIDLSTLVPTSIQGFLYPGGFINFQVSSARYAFPFSVSWRRHHYTFPAQTIIQVLPRLRFELG
    DWFTILQKSVGKWVTLYQHDMIIPERIEGVLSLNNTATLMSHHNQTTHMLGVENRGDLSLKVLGILQSGQ
    IKGSKSENSASGHFSKLLSVPAHTIKNLSFKEEEGTKSKNILFYSVLEETFLKATDKPLTIIKRDKWSLY
    DEIQVFATTLNLQNFLRYHISEETPMLSRLLMYAQKRVSIQNRDLALKFFYVRERSGIGAIRLVFKNFFE
    ESLQGILHGTLEREVKPMLAENPNTLIHSSYRNHLELILGEETLDLAIIVQEFSSSKNIVELQKDLATHR
    LIYPQGKEALSLAIYTHTFSKESLVTSKQRSEGLKFIDPSMQEYRLPETTILENSYYLDPSTGDLYLTKI
    IAQPSQSRAFLIKFKKYKRHWLDFQKLVLSGSHLELIASTGTALTFVGPELRHLEIDFPGALNGTIAKEH
    ISSRASIVIPTNNQVTHYDPRIAKQHYSSINYMLWDLRDRATLSKRAKTLDSYLLEVCMNLDSSNPTWNI
    PNDVLNYAVGYYRTILPSWVKNKIRVGSLIKVEADTAKTLSFSLITTQNDLFQPVVDKGFYIYYSVSKLA
    NHVKLRNVTGDMLLDIDKETTFIVRGVDESDYDKKQIYVVLDLTTEEERKLRTDKNIIIIPGGERLNR
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0439 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
  • Related Vaccine(s): C. muridarum DNA vaccine encoding TC0439
11. TC0512 outer membrane protein, putative
  • Gene Name : TC0512 outer membrane protein, putative
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • VO ID : VO_0010885
  • NCBI Gene ID : 1245872
  • NCBI Protein GI : 497916494
  • Locus Tag : TC_RS02600
  • Genbank Accession : AE002160
  • Protein Accession : WP_010230650
  • Taxonomy ID : 243161
  • Gene Starting Position : 618235
  • Gene Ending Position : 620613
  • Gene Strand (Orientation) : +
  • Protein Name : outer membrane protein assembly factor BamA
  • Protein pI : 9.51
  • Protein Weight : 84655.32
  • Protein Length : 792
  • Protein Note : similar to GB:X75201, and PID:435005; identified by sequence similarity; putative
  • DNA Sequence : Show Sequence
    >NC_002620.2:618235-620613 Chlamydia muridarum Nigg, complete genome
    AATGCTTGGAATACGAAAAAAAACGATTCTGCAACTCGCTATTTTACTGTTGCTCCCCTTTTCACAAAAC
    GCCTTTGGAACATCTCCTGAAGGACGTATGGTGGTACAATCCATCACTATTGAGACTCGAGGAGAAAATG
    CTCAAAATAAGCGGGCTATTCCCAAAATAAAGACAAAGCAAGGGACGCTGTTCTCTCAAACTGATTTTGA
    CGAAGATCTAAGAACTCTCTCGAAAGATTTCGACCGCATTGATCCTATTGTAGAGTTTCGCAATGGGCAA
    GCTGTTATTTCCCTTATTCTGACAGCAAAACCCGTTATCCGACACATTAACATCTCAGGGAATGAAGCAA
    TTCCTACTCATAAAATTTTAAAAACCCTAGAGCTTTATAAAAACGATCTTTTTGATCGAGAGGTTTTTTT
    CAAAAATTTTGATGCTTTAAGAACCCTTTACTTAAAACGCGGATACTACGATTCTCAATTGTCCTATTCT
    CACGATCATAATGAGCAAGAAGGTTATATTGATATCTCAATCCAAATTAAAGAAGGGCCCTACGGACGGA
    TAAAAAAATTAACAATTTCAGGTATTACTCGTACAGAAGCCTCTGATTTGGGAGACATAGTTTTAACCAA
    ACAGTACTCTACAACAACAAGTTGGTTCACTGGAGCAGGGGTATACCATCCAGACATGGTGGACCAGGAT
    CTCTTTGCCATAACAAACTATTTTCAAAATAAAGGTTATGCAGACGCAAAAGTCACAAAAGAAGTCTCCA
    CAGATGCTAAAGGAAACCTCTCTCTTGTTATCAACGTAGATAAAGGGCCCCTGTATACTTTAGGTCACGT
    ACATATAGAAGGTTTCACAGCTTTATCCAAGCGGTTACTTGATAAACAACTGTTAGTGGGCCCCAATTCC
    TTATATTGCCCAGAAAAAGTTTGGGCTGGAGCTCAAAAAATCCGTAATGCCTACGCTAGATACGGATATG
    TAAATACCAATGTAGACGTATCTTTTGCTGTTCATCCAACGCTCCCAGTTTATGATGTTACTTACAGGGT
    TAGCGAAGGAGCTCCTTACAAAATCGGACTAATCAAAATCAAAGGGAACACACACACCAAACATGACGTA
    ATTTTACATGAGACAAGTCTGTTCCCTGGAGATACTTTTGATAGATTAAAACTAGAAGATACGGAAACTC
    GATTACGTAACACAGGCTACTTTAAAAGTGTGAGTGTCTACACGGTACGCTCACAATTAGATCCTTTGGA
    CTCAAATAACCTTTATAGAGACGTTTTCATTGAAGTCAAAGAAACCGAAACAGGTAACCTAGGACTGTTC
    TTAGGATTCAGTTCTATAGACCATCTGTTCGGAGGAGCAGAAATTTCAGAAAGCAATTTTGATTTATTTG
    GCGCCCGACACTTATTCAAAAAAGGGTTTAAATCCTTAAGAGGTGGTGGGGAATATCTTTTCTTAAAAGC
    CAATTTAGGAGACAAAGTTACTGATTATACTGTTAAATGGACAAAACCTCATTTCTTAAACACCCCATGG
    ATCCTAGGAGTAGAATTAGATAAATCTATTAATAAGGCTTTATCAAAAGATTATTCCGTTGATACCTATG
    GAGGAAACATCAGCACGACATACATTCTTAACGACAAACTGAAATATGGGATGTATTACCGCGGGAGCCA
    AACGAGCCTAAGTTTAAGAAAAAAAACAGAAAATCCTAACAAACCTGGACCAGATCTAGATAGTAACAAG
    GGATTTGTTTCCGCGGCAGGGCTTAACGTTCTTTACGATTCTATTGACAATCCTAGAAAACCTACTATGG
    GTATTCGGAGCTCTTTGAACTTTGAGTTGTCTGGATTAGGAGGAACCTATCAGTTTACAAAATTAACAGC
    CAGCGGCTCTATCTATCGCCTGCTAACTAAAAAAGGTGTTTTGAAAATTCGTGGAGAAGCTAAGTTTATT
    AAACCTTTCGGTACAACCACAGCCCAAGGAATTCCTGTTAGTGAGCGCTTCTTCTTAGGAGGAGAATCTA
    CCGTTCGTGGATATAAACCATTTATTATTGGGCCTAAATTTTCTCCAACAGAGCCTCAAGGGGGCTTATC
    TTCCTTATTGCTAACAGAAGAGTTTCAATATCCTCTAATTTCTCAACCCAGCATCAATGCATTCGTTTTT
    CTAGATTCGGGCTTTATTGGCACGGAAGAGTACACTATTCGACTCAAAAATCTCTGTAGCAGTGCCGGAT
    TTGGGTTGCGTTTTGATATGATGAATAACGTGCCCATTATGTTAGGTTGGGGTTGGCCTTTCCGCCCAAC
    AGAAATTCTTAATAATGAAAAAATTGATGTATCTCAAAGATTCTTCTTCGCTTTGGGAGGAGTGTTCTA
    
    
  • Protein Sequence : Show Sequence
    >WP_010230650.1 outer membrane protein assembly factor BamA [Chlamydia muridarum]
    MLGIRKKTILQLAILLLLPFSQNAFGTSPEGRMVVQSITIETRGENAQNKRAIPKIKTKQGTLFSQTDFD
    EDLRTLSKDFDRIDPIVEFRNGQAVISLILTAKPVIRHINISGNEAIPTHKILKTLELYKNDLFDREVFF
    KNFDALRTLYLKRGYYDSQLSYSHDHNEQEGYIDISIQIKEGPYGRIKKLTISGITRTEASDLGDIVLTK
    QYSTTTSWFTGAGVYHPDMVDQDLFAITNYFQNKGYADAKVTKEVSTDAKGNLSLVINVDKGPLYTLGHV
    HIEGFTALSKRLLDKQLLVGPNSLYCPEKVWAGAQKIRNAYARYGYVNTNVDVSFAVHPTLPVYDVTYRV
    SEGAPYKIGLIKIKGNTHTKHDVILHETSLFPGDTFDRLKLEDTETRLRNTGYFKSVSVYTVRSQLDPLD
    SNNLYRDVFIEVKETETGNLGLFLGFSSIDHLFGGAEISESNFDLFGARHLFKKGFKSLRGGGEYLFLKA
    NLGDKVTDYTVKWTKPHFLNTPWILGVELDKSINKALSKDYSVDTYGGNISTTYILNDKLKYGMYYRGSQ
    TSLSLRKKTENPNKPGPDLDSNKGFVSAAGLNVLYDSIDNPRKPTMGIRSSLNFELSGLGGTYQFTKLTA
    SGSIYRLLTKKGVLKIRGEAKFIKPFGTTTAQGIPVSERFFLGGESTVRGYKPFIIGPKFSPTEPQGGLS
    SLLLTEEFQYPLISQPSINAFVFLDSGFIGTEEYTIRLKNLCSSAGFGLRFDMMNNVPIMLGWGWPFRPT
    EILNNEKIDVSQRFFFALGGVF
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0512 showed significant levels of protection when compared to negative control animals. At 9 days following challenge TC0512 showed a 73% reduction in the number of recoverable Chlamydia compared with vector only immunized controls (McNeilly et al., 2007).
  • Related Vaccine(s): C. muridarum DNA vaccine encoding TC0512
12. TC0757 hypothetical protein
  • Gene Name : TC0757 hypothetical protein
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • VO ID : VO_0010886
  • NCBI Gene ID : 1246122
  • NCBI Protein GI : 497917305
  • Locus Tag : TC_RS03850
  • Genbank Accession : AE002160
  • Protein Accession : WP_010231461
  • Taxonomy ID : 243161
  • Gene Starting Position : 898853
  • Gene Ending Position : 899647
  • Gene Strand (Orientation) : +
  • Protein Name : hypothetical protein
  • Protein pI : 5.02
  • Protein Weight : 27979.63
  • Protein Length : 264
  • Protein Note : identified by match to TIGR protein family HMM TIGR01607
  • DNA Sequence : Show Sequence
    >NC_002620.2:898853-899647 Chlamydia muridarum Nigg, complete genome
    CATGCGCTGTTCTGCTTATTGCTCTGCTTCGTCCTATCATCTACACGTTCTTTTTCATCTTTTAAAGGCA
    CGCTACCCTTCTGTCTTATCCCGAGAATACGTGCTCATTTCCTCTGAAGAGCTGAATGAAAGCGACAAAG
    CCGCTGTCTTTTTCCCATTTGGAGTTAGTGTGTTTTGGGGATGGGAAGAAGCTGAAGAACTTCAAGTGAT
    CCAAGCTATCACCTCAGCATCAGTGGATCCTCTCCCTAACCCAGAAATTGATAGTTATGACTTCCATTAT
    GGAGAGAAACTACAGATCCGTCGCGATAGACTTGTTCTCACCAACTCTAATTTAAACACAAAACTTGCTA
    TTTCCTTTGGGTTAGCCCAATCTATCAAACTTACCGTCTTTGAAGAAACGATTTACAAAACAGTTGAAAA
    CTCTAAATCTTTGCCTCAAGAACTTGCCTCTAAAGGGAAAATTTCTCTATCCCGTAAAACGATCGCAAAA
    AAGATTGGAGAGCTTTTCCTGGATAAAGCTTCCGTAAACCTACACTCTGACATTCTCGATGAGCCCGACT
    TTTTCTGGGAACATCCAGAAACCCAACCTTTTTATATTGATGTGTTGACCTGCCTAGATGTAAACGCGCG
    AGTAAATGTATTAAATCACAGACTTGCTATCCTAGGAGATGTTCTCGAGATTTTAAATGACCAACTTAAT
    CATCAGCATTCTTCAGCTCTAGAGTGGACCGTGATCTGGTTAATTGCACTAGAGGTATTGGTCACCTTAC
    TTAAAGACGTATTCAACATCATCTA
    
    
  • Protein Sequence : Show Sequence
    >WP_010231461.1 RMD1 family protein [Chlamydia muridarum]
    MRCSAYCSASSYHLHVLFHLLKARYPSVLSREYVLISSEELNESDKAAVFFPFGVSVFWGWEEAEELQVI
    QAITSASVDPLPNPEIDSYDFHYGEKLQIRRDRLVLTNSNLNTKLAISFGLAQSIKLTVFEETIYKTVEN
    SKSLPQELASKGKISLSRKTIAKKIGELFLDKASVNLHSDILDEPDFFWEHPETQPFYIDVLTCLDVNAR
    VNVLNHRLAILGDVLEILNDQLNHQHSSALEWTVIWLIALEVLVTLLKDVFNII
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0757 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
  • Related Vaccine(s): C. muridarum DNA vaccine encoding TC0757
13. TC0767 hypothetical protein
  • Gene Name : TC0767 hypothetical protein
  • Sequence Strain (Species/Organism) : Chlamydia muridarum str. Nigg
  • VO ID : VO_0010888
  • NCBI Gene ID : 1246132
  • NCBI Protein GI : 497917334
  • Locus Tag : TC_RS03895
  • Genbank Accession : AE002160
  • Protein Accession : WP_010231490
  • Taxonomy ID : 243161
  • Gene Starting Position : 910619
  • Gene Ending Position : 911350
  • Gene Strand (Orientation) : -
  • Protein Name : polysaccharide deacetylase
  • Protein pI : 9.14
  • Protein Weight : 26828.18
  • Protein Length : 243
  • DNA Sequence : Show Sequence
    >NC_002620.2:910619-911350 Chlamydia muridarum Nigg, complete genome
    ATTAATGCAAAATAAGGAATCGTTTTAATTGGCGGAAAGAGAGCTGATAAAGAGATGGGATCCCTCGACT
    ATCTAAAGCCATATCAATACGAGGAATGCTATGCTGTGATTGCATCGAGTAAAAAAAACTCGCAGCATCC
    CCTAACACAAAAGAATAGCGGTAAACCTCCTGAACAAAATGTTGACTCACCAAATCACTTTTTCCAAAAG
    GATAAAAAAAGACTTCCACAGGAGCCTGTATAGCCTCTTCTAGTAAAATTTTAGACAATGAAATTTCAGT
    ATGTAAATAAGGAGGGGAGTTTTTAAGGTTTCTAATAGCAAACCCTGAAGAGGCAAATCGAATAATCGAA
    CTTTGAGCCATATGACAAAGTTCTTTAACCGAACAAAAGGGCTGATAATAAGAAAATATTTCGTCTTGAA
    AAGCTAGAAAATCCGATGGCGAAATTCGCATATCAATGGGAAGATTTTCACTTTCTAAACGGGATACGTA
    TCTCCAAGCAACTCCAACAACCGCAGGAATCTGGAATTTCTGAAGAAAAGGAAAGACATGCTTGTAAAAA
    TCAACAGAAGCATAATCGAATGTCAGTATGATTGCTTTCCTATTAGGGAGAGGATCTCCGGGCAGAATAA
    ACGAATAATGCTGCTTGAGAGAGTGCAAAAAATCTAAAAACGATCTGAGGGCATAAGGAAACTTAGAAAA
    AGCAACCTGTCGATAGGCTAAGACGCGGAGCA
    
    
  • Protein Sequence : Show Sequence
    >WP_010231490.1 polysaccharide deacetylase family protein [Chlamydia muridarum]
    MLRVLAYRQVAFSKFPYALRSFLDFLHSLKQHYSFILPGDPLPNRKAIILTFDYASVDFYKHVFPFLQKF
    QIPAVVGVAWRYVSRLESENLPIDMRISPSDFLAFQDEIFSYYQPFCSVKELCHMAQSSIIRFASSGFAI
    RNLKNSPPYLHTEISLSKILLEEAIQAPVEVFFYPFGKSDLVSQHFVQEVYRYSFVLGDAASFFYSMQSQ
    HSIPRIDMALDSRGIPSLYQLSFRQLKRFLILH
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
  • Related Vaccine(s): C. muridarum DNA vaccine encoding TC0767/768
14. TC0768 hypothetical protein
  • Gene Name : TC0768 hypothetical protein
  • Sequence Strain (Species/Organism) : Chlamydia muridarum Nigg
  • VO ID : VO_0010889
  • NCBI Gene ID : 1246133
  • NCBI Protein GI : 15835382
  • Locus Tag : TC_0768
  • Genbank Accession : AE002160
  • Protein Accession : NP_297141
  • Taxonomy ID : 243161
  • Gene Starting Position : 911335
  • Gene Ending Position : 911496
  • Gene Strand (Orientation) : -
  • Protein Name : hypothetical protein
  • Protein pI : 3.69
  • Protein Weight : 5224.4
  • Protein Length : 53
  • DNA Sequence : Show Sequence
    >NC_002620.2:911335-911496 Chlamydia muridarum Nigg, complete genome
    GCTAAGACGCGGAGCATGGATCAACTGAAGGAATTGCAAAAGGGTCCTCTGGATAAATGACTACCTGTCC
    GGAAATGATCGTTAGATTACGCTCTTTTCCCTCAGGAGTAAGCTCTGCTGTTTCCGTAGAACAGACTTCA
    GAAGTAGGTAAGGAGATCGGCA
    
    
  • Protein Sequence : Show Sequence
    >NP_297141.1 hypothetical protein TC_0768 [Chlamydia muridarum str. Nigg]
    MPISLPTSEVCSTETAELTPEGKERNLTIISGQVVIYPEDPFAIPSVDPCSAS
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
  • Related Vaccine(s): C. muridarum DNA vaccine encoding TC0767/768
15. Virulence plasmid protein pGP3-D
  • Gene Name : Virulence plasmid protein pGP3-D
  • Sequence Strain (Species/Organism) : Chlamydia muridarum
  • NCBI Protein GI : WP_010231988
  • Other Database IDs : CDD:283197
  • Taxonomy ID : 83560
  • Protein Name : Virulence plasmid protein pGP3-D
  • Protein pI : 4.33
  • Protein Weight : 26436.35
  • Protein Length : 340
  • Protein Note : Chlamydia virulence protein PGP3-D; pfam05475
  • Protein Sequence : Show Sequence
    >WP_010231988.1 Virulence plasmid protein pGP3-D [Chlamydia muridarum]
    MGNSGFYLHNTSNCVFADNIKVGQMTEPLTDQQIILGTSTTPVAAKITASEGISLTITNNAQANSSVNIG
    LDAEKAYQLILDKLGDQIFDGITGSIVESAVQDIIDKITSDPSLGLLKAFYNFQITGKIQCNGLFTSSNV
    TTLLGGTEIGRFTVTPRSSGSMFLVSADIIASRMEGGVVLALVKEGDTQPCAISYGYSSGVPNLCSLKTC
    VTNSGSTPTTYSLRIGGLESGVVWVNALSNGNDILGITNTSNVSFLEVIPQKNT
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation :
1. Ifng (Interferon gamma)
  • Gene Name : Ifng (Interferon gamma)
  • Sequence Strain (Species/Organism) : Mouse
  • NCBI Gene ID : 15978
  • NCBI Protein GI : 33468859
  • Genbank Accession : NM_008337
  • Protein Accession : NP_032363.1
  • Other Database IDs : MGI:107656; UniProt: P01580
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence
    >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
    ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
    GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
    GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
    AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
    AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
    GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
    AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
    GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
    AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
    AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
    AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
    TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
    TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
    ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
    GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
    AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
    TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
    AGTATACCTTTGCAACC
  • Protein Sequence : Show Sequence
    >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
    MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
    ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
    LLPESSLRKRKRSRC
  • Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
  • Additional Molecule Role : Vaximmutor
  • Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
  • Related Vaccine(s): C. muridarum MOMP protein vaccine
2. IgA
  • Gene Name : IgA
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 238447
  • Genbank Accession : AC160982
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 8607
  • Gene Ending Position : 12639
  • Gene Strand (Orientation) : -
  • Protein Name : immunoglobulin heavy constant alpha
  • Protein Note : Also known as IgA; Igh-2
  • DNA Sequence : Show Sequence
    >gi|121699722:8607-12639 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
    AGTACTGGGGGACCTCTTTGCTGCCAAACGGGCCTCGAACAGTTGTAACAGTGAGTGCTGTGCTGTAGAA
    CAAGCTCAGTAGGAAGAGGGTGAGGAAGGTCACAGTGGTGGGCCACAGGCTGGCACCTGGGGCCTCTTCC
    TCCAGGATGTCTTCTGACTGGTCCAGTAGCACATAGGAAAGTGGCTCTTGACGTTCTGTAAGACATAGAT
    GACCTTCTAGATCCAAACCCATGGGGATGGGGGCTCAGACTTAAGGTTTCTGCTTCTATGCCTCCTTGGG
    CTTTGGGGACAGTGGTGGTAGGGGTCCCTTATTCCCGGGAAGGGACTTGTTCAGGGATAATGAGGAGGCA
    GGGTCCCAGGTAAGGGACTGGAATCCCAGCAGTTCTTGAGATGGCCCCAAGACCCATCTCTCTCTCTTAG
    GGAAACCTTAATTAGAGACCTGTCATGGTAAGAGAGTTGTTTCTGTCAAACTCTGAGAAGAGTTTGAACC
    CAAGGCTCCTTAGGTCTCAGTCCTTTCTAGATATTTAGCAATACCTCCCTAAACCCCAGTTCTTAGGCTA
    ATCTTGGCTTGGGTACTAGGGTTGTGTAAGATCAGACACCTTCAAACAGATACAGGCCAATGTGATTTCT
    GGGCCAAAGTTAGACTACCAAGGCCTGGACATGTGGTCAGAGGACTCTGTTGCATTCTTAAGAGCTGTGA
    CTGGGGGTTAAGCAACAGTAAAGACCCCCAGTAGAGCCAACCCTGCTGTCCTCAGGTTCTCTGCGCACAG
    AGTTTCATTTTAAGTGGCAGTAGGAAATGTGTACTGATAGTCAACCACTCGTATTTCTGTCACTGCAGCC
    ATCCATCAATCCATCCATCATCCCCATCGTTCATCCTTGTAGCCAGCCATCCATTACCCTCCAGCCGCTC
    ACTCATCATCTTTCCCGACTATCGTGATTCTCCAGCTTCTCTGCCCCCTCTCCCTGTGGGGATTCAGTCT
    TTCACCTATTGACTCCTCCTTACTAGGATTCTCTGTCATGGGCCAGGCCTTGGATTCCATGGGTGGCTAT
    GGTACTTTTCTGAGTCAGGTTTCCCAAGCTGGTCTGTTGCTCTTGCTATTCTCAGGGAACTGGACTTTCA
    AGCTGGAGAGTATCCAGGGCATGCATGGGCCTTTACTCCACTCTTGCTCTGAGAGCAGAGGGAAGTTGCT
    AGAAACCAGCAACTCCTCCCCCCCCCCCCCGAAGTTCTGAAGGCCTGTGAGACAGGAGCAGGGCCCCCAC
    TGCCCTCTTGTGGACACCTGGGCTCCAAGTATACCTGGTTGTAGAACTTTGTGTTCTAGGGGTGCCCATG
    GAGGAGGCCGAGGCCTGTGTGGGAGTGGTCTCCATGGCCTGCTCCTGCTTCTTCCCAGATTTGCCTCTTA
    TCCTGCTTCTGGTTTGTGATACTTGGGCTCAGGATGGGAAGGGAGACTTAGGTTAGAAAGGGGGCACCTG
    GAGGGGCCTATGCCCTTGTCCATTGTGTGTAGTTGTCAGTGTCGGGCCTGGAAATTCTCCCGGGGGCTTG
    GAGGGGACTCCCCTTGCTTTCCAGAAGCTGGGTGTGGGCTGTGAACTTCATCTTCCTCCAGGGTTCAGGT
    CTGTGTGAGTGTGCAAATGTGAGTGTCTGTGCATGTATGCCCATGTGCATGTATGCAGAACAGGGTGTAT
    GCCCATGTGCATGTGTGAAGAGCAGGGTGCTGCTGAGGGTTGTCTGTGCCATGCTATATGTCTTTCTCAT
    GAGTCAGTGGTCACCCTGAATGCATGTTTCCATTCAGGGATGGCCTACTGAGGGTGCGTGGCATCTTCTT
    CCCAGTGCCCCTGTGTTCATCCCTTCATTCTCATACCATCATCTCTATTGTGACCCCCACACGGCACCAG
    CCTTGATGGTGCTACCCTTTTGCTCCATCTATCCCTGTCTTGTTTGGGTTAGAAGCTGCGGTCTAATAAA
    CAGCCACACCACCCACCACTCAGAGTACTTGTGCAGCAACCCACTGCCCTGGGGACTCTCCCATGAGCTT
    TGCCTTCTTGGCTGAGTCTGCTCGCCTTGGATTTCATCCCCAGTTTATTCTTACTTCCCATTCACTCATT
    GCACATTCACAGGGCAGGTGCACACACTCTCATGTTTCCACCAACATGAACACACATAGATATATGCATG
    CTCATAGGTATGCACAGGCACACACTTGCTCACAGGGACATAGATATAGTGTGTCCACATATGTACTTAT
    ACCCATATACATACCTATAAACCATAGGTGCACACAAACTCACTCCTCTCTTGCTGCACACAGGTACACA
    CATGAAGGCACATGGACCCATCCATAAGCACTCACCAATACATAGACATGCATACACATATACTCTAGGC
    ATGAAGGGTCTTCAAAGTCTCCGAGGCCTTACAAGCTTAGGTGGTGGTGTTTCTCCCTCCCTGGGCCCTG
    ACCCCTCCCTGTGTTCTGTAGACCCTAAGGATGATGGACAGGCACTGGATGGAAGTGCAGGGATACTTTG
    GATGAGCACAGAGTTTATTTCAGGAGTAGGGACAGGCAGGGTGGCTCAGTAGCAGATGCCATCTCCCTCT
    GACATGATCACAGACACGCTGACATTGGTGGGTTTACCCGACAGACGGTCGATGGTCTTCTGGGTGAAGT
    TCATGGGCAAGGCCTCGTGGCCCACCATGCAGGAGTACTGGTCACCCTGTTTCCAGAGTTCAGCTGATAC
    ACGCAACACGCTTGTCACCAGGTAGGTGGTGGCTCCCTCGCCTGGCTCCTTTAGGGGCTCAAACACTAGG
    TAGCTTTCTGGGGACAGCTCCTCATTTCCATGCAGCCATCGCACCAGCACTTCTTTAGGGTTGAAAGCTC
    GCACCAGGCATGTCAGGGACACGAGCTCATTCAGGGCCAGCTCCTCCGACGGCGGCGGTAGCAGGTGGAC
    CTGGGGTGGGAAGGTGTTCACTGGAAGGTAGAAAGAAGAGTTATGGCTCAGACAAGGAGAGCAAGACCCC
    TCTGCCCTTCCCTTCTGGGCAGTTCCTCAGATAGCTCCAATTTCCCTCTGTGATATAAGGACAGGAGCAG
    TTAGTTGAGCATCTGTATTATAGGAAGAAAGCAGGCATGTGAGCAGGGAACGTCATACAATGTCCCGGGT
    ATGCATCTGGGCTCACCTGTGATTTTGGCAATTGTGCCAGTTAAGGTGTCAGACTCAGGATGGGTAACTG
    TGCACTTGAATGATGCGCCACTGTTCCAGCGCTCAGCACAGCCAGGCAGGACGCTGGACACACTGTAGCA
    GCCGCAGGAATTCTGCACAGCTTTCTTCTGCACTGCATCCTTCCCAGTGGAGGGCTCCCAGGTGAAGACA
    GCTCCCTCAGGATTTCTCAGGCCATTCAGAGTACATGTGAGGCTGGCATCTGAACCCAGGAGCAGGTCCT
    CAAGAGCTGGCCGCTGCAGTGACAGGCTGGGATGGCAGGAAGGAGGACAAGGAGGACAAGGAGGAGGAGG
    ACCTGTAACAGAGAAGTCCTAGCAAATCAGTATATTCTCCTTTTCTCCTTTGCTGTCCCCTTTTCTGGAT
    GTTCTGAGGCCTCAGTTCCTTATGGCCTGTTTACGCTTCCCTTCCCCCACCCCCATGGGAGGTTTCTGAG
    GGTCCCTGTGAGGGTGTGCCTCAGAGGGATATATGGATCTAGATAAGGTAGAACTTATCCCACCCCCAGC
    TGACCCCCTAACGTTCTTTACCAGAGCACTTCACATCCAATTCTTGGACGGCGTTAGAGTCATGTTGCAC
    GGAACATTTCACGGATTCTCCTTCTGGGCACTCGACAGCTGGCAGGGTCAACTGGCTGCTCATGGTGTAC
    CCTCCCCCAGAGGCCAGGGCAGGTGGGAAGTTTACGGTGGTTATATCCTTCCCACTCTTTCCCCAGGTCA
    CATTCATCGTGCCGGAAGGGAAGTAATCGTGAATCAGGCAGCCGATTATCACTGGGTCACTTGACAGAGC
    TCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGAC
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): C. muridarum MOMP protein vaccine
3. IgG
  • Gene Name : IgG
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16059
  • Genbank Accession : AF010213
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 881411
  • Gene Ending Position : 914690
  • Gene Strand (Orientation) : +
  • Protein Name : immunoglobulin heavy chain (V7183 family)
  • Protein Note : Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)
  • DNA Sequence : Show Sequence
    >gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
    CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
    GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
    GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
    TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
    TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
    CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
    CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
    ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
    CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
    CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
    AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
    CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
    AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
    TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
    AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
    AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
    ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
    TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
    GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
    TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
    AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
    TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
    GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
    TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
    TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
    TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
    ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
    CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
    GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
    CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
    TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
    GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
    TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
    CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
    AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
    TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
    CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
    AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
    TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
    CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
    AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
    AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
    TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
    AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
    ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
    CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
    ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
    GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
    ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
    AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
    ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
    ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
    CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
    CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
    CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
    GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
    ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
    CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
    CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
    AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
    GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
    TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
    AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
    GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
    GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
    ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
    TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
    AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
    ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
    CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
    TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
    TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
    TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
    CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
    AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
    ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
    CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
    AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
    GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
    ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
    CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
    TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
    TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
    TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
    CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
    AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
    AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
    CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
    CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
    TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
    AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
    CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
    ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
    CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
    AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
    TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
    TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
    GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
    GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
    AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
    CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
    AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
    GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
    ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
    TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
    TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
    GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
    GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
    GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
    TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
    TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
    AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
    GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
    GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
    CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
    CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
    ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
    CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
    ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
    GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
    GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
    GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
    TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
    TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
    CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
    TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
    GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
    TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
    CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
    AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
    TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
    AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
    CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
    CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
    TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
    GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
    GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
    ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
    GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
    GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
    GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
    GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
    ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
    TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
    AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
    AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
    AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
    GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
    CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
    GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
    TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
    TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
    CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
    AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
    TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
    TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
    AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
    TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
    GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
    CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
    TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
    TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
    TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
    AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
    GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
    AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
    TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
    GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
    TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
    TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
    TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
    CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
    CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
    TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
    ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
    TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
    ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
    TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
    ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
    AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
    TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
    AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
    CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
    CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
    GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
    CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
    GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
    TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
    CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
    TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
    AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
    GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
    ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
    GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
    TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
    CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
    CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
    ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
    GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
    TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
    ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
    CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
    ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
    TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
    GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
    TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
    GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
    TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
    GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
    ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
    CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
    CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
    ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
    AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
    ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
    AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
    GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
    TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
    GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
    GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
    TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
    CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
    GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
    TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
    CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
    TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
    TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
    TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
    GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
    CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
    AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
    GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
    ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
    TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
    AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
    TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
    AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
    CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
    GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
    TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
    TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
    TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
    GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
    ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
    ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
    CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
    GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
    TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
    CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
    GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
    GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
    AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
    CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
    TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
    GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
    ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
    TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
    AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
    AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
    ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
    TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
    GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
    TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
    TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
    TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
    AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
    ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
    AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
    GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
    CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
    AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
    TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
    TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
    TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
    GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
    GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
    ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
    AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
    AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
    GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
    TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
    GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
    ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
    AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
    TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
    GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
    CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
    TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
    TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
    GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
    ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
    CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
    ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
    TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
    TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
    TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
    CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
    CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
    GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
    AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
    AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
    GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
    ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
    TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
    TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
    CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
    TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
    AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
    TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
    AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
    TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
    GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
    TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
    ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
    ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
    CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
    TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
    TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
    TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
    TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
    TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
    GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
    CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
    AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
    CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
    CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
    CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
    GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
    TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
    CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
    GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
    TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
    GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
    TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
    AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
    TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
    CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
    AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
    CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
    TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
    GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
    CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
    ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
    ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
    TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
    GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
    CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
    GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
    GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
    GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
    ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
    TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
    CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
    TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
    TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
    ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
    TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
    GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
    CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
    TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
    CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
    GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
    ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
    CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
    CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
    GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
    AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
    AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
    ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
    ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
    GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
    ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
    CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
    CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
    TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
    CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
    CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
    TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
    TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
    GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
    TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
    AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
    TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
    GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
    CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
    CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
    TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
    GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
    GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
    CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
    AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
    TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
    CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
    TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
    AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
    TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
    CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
    AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
    TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
    CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
    TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
    GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
    ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
    TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
    TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
    AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
    CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
    TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
    GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
    GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
    TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
    TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
    AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
    AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
    TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
    CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
    ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
    ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
    ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
    GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
    TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
    ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
    TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
    GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
    AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
    TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
    TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
    TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
    GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
    AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
    CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
    TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
    GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
    CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
    ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
    AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
    CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
    CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
    ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
    GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
    ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
    AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
    AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
    GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
    CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
    AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
    ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
    ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
    CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
    TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
    CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
    TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
    CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
    AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
    GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
    GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
    GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
    TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
    TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
    TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
    ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
    ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
    AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
    TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
    CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
    CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
    TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
    AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
    CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
    CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
    GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
    ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
    CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
    ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
    TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
    ACAAGCACAGTGCAAATTTCTGTCACCCTG
  • Molecule Role : Vaximmutor
  • Additional Molecule Role : Vaximmutor
  • Related Vaccine(s): C. muridarum MOMP protein vaccine
IV. Vaccine Information
1. C. muridarum DNA vaccine encoding TC0439
a. Vaccine Ontology ID:
VO_0011533
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
C. muridarum TC0439 adherence factor
e. Gene Engineering of TC0439 adherence factor
  • Type: DNA vaccine construction
  • Description: The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
  • Detailed Gene Information: Click here.
f. Vector:
pCI30
g. Immunization Route
Subcutaneous
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007).
  • Challenge Protocol: All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
  • Efficacy: The groups of female BALB/c mice immunized intra-abdominally with TC0439 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
2. C. muridarum DNA vaccine encoding TC0512
a. Vaccine Ontology ID:
VO_0011534
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
C. muridarum TC0512 outer membrane protein, putative
e. Gene Engineering of TC0512 outer membrane protein, putative
  • Type: DNA vaccine construction
  • Description: The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
  • Detailed Gene Information: Click here.
f. Vector:
pCI30 (McNeilly et al., 2007)
g. Immunization Route
Subcutaneous injection
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007).
  • Challenge Protocol: All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
  • Efficacy: The groups of female BALB/c mice immunized intra-abdominally with TC0512 showed significant levels of protection when compared to negative control animals. At 9 days following challenge TC0512 showed a 73% reduction in the number of recoverable Chlamydia compared with vector only immunized controls (McNeilly et al., 2007).
3. C. muridarum DNA vaccine encoding TC0757
a. Vaccine Ontology ID:
VO_0011522
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
C. muridarum TC0757
e. Gene Engineering of TC0757 hypothetical protein
  • Type: DNA vaccine construction
  • Description: The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
  • Detailed Gene Information: Click here.
f. Vector:
pCI30
g. Immunization Route
Subcutaneous injection
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007).
  • Challenge Protocol: All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
  • Efficacy: The groups of female BALB/c mice immunized intra-abdominally with TC0757 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
4. C. muridarum DNA vaccine encoding TC0767/768
a. Vaccine Ontology ID:
VO_0011524
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
C. muridarum TC0767/768
e. Gene Engineering of TC0767 hypothetical protein
  • Type: DNA vaccine construction
  • Description: The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
  • Detailed Gene Information: Click here.
f. Gene Engineering of TC0768 hypothetical protein
  • Type: DNA vaccine construction
  • Description: The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
  • Detailed Gene Information: Click here.
g. Vector:
pCI30
h. Immunization Route
intra-abdominal injection
i. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007).
  • Challenge Protocol: All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
  • Efficacy: The groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
5. C. muridarum MOMP protein vaccine
a. Vaccine Ontology ID:
VO_0011453
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
C. muridarum major outer membrane protein MOMP
e. Gene Engineering of MOMP
  • Type: Recombinant protein preparation
  • Description: Recombinant maltose binding protein-MOMP (MBP-MOMP) was cloned into the bacterial expression vector pMAL-c2, and used to transform Escherichia coli (DH5α[pMMM3]). The MBP-MOMP was purified by passage through a PD-10 column (Amersham Biosciences, NSW, Australia), followed by a Sephadex G-75 column (Sigma–Aldrich) (Skelding et al., 2006).
  • Detailed Gene Information: Click here.
f. Adjuvant:
g. Adjuvant:
h. Immunization Route
Intranasal immunization
i. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Groups of five mice were immunized on days 0, 7, 14, and 35 via either the IN or TCI route. One group of five mice were used as a control, receiving no immunization solution. Mice immunized intranasally were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia), and 10 μl of immunization solution containing 100 μg MOMP, admixed with 10 μg CpG-ODN 1826 (5′-TCC ATG ACG TTC CTG ACG TT-3′) (Geneworks, SA, Australia), and 10 μg cholera toxin (Sigma–Aldrich) was gently pipetted onto each nare (5 μl per nare) (Skelding et al., 2006).
  • Challenge Protocol: Five days after the final boost, mice were infected intranasally with C. muridarum. Mice were anaesthetised with 4% isoflurane and 10^3 IFUs were gently pipetted onto the nares. Mice were weighed daily for 10 days then sacrificed 10 days post-infection via a sodium pentobarbital overdose (Nembutal, Abbott Australasia). The lungs from the mice were either perfused and fixed for histopathology or collected for determination of C. muridarum levels (Skelding et al., 2006).
  • Efficacy: Both intranasal and transcutaneous immunization protected mice against respiratory challenge with Chlamydia (Skelding et al., 2006).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: Both TCI and IN immunization resulted in greatly increased IFN-γ gene expression in stimulated lymph node cells (stimulated with MOMP) as compared to non-immunized mice (Skelding et al., 2006).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgA
    • Gene Response: Both routes of immunization induced a significant MOMP-specific IgA response in serum with the highest IgA response seen in animals immunized intranasally. These results were compared 7 days post-immunization to non-immunized control mice (Skelding et al., 2006).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Both routes of immunization (TCI and IN) resulted in levels of MOMP-specific IgG in serum that were significantly higher than that in serum of non-immunized control mice. There was no significant difference between serum IgG levels in animals immunized by either route. These results were gathered 7 days post-immunization (Skelding et al., 2006).
    • Detailed Gene Information: Click here.
6. C. muridarum PmpE/F-2 protein vaccine
a. Vaccine Ontology ID:
VO_0011456
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
C. muridarum polymorphic membrane protein E/F family protein
e. Gene Engineering of PmpE/F-2
  • Type: Recombinant protein preparation
  • Description: The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: pmpE/F-2 and gap DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pET32a (Novagen) for pmpE/F-2 and gap after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques. Only the first half of the gene (25–575) was cloned into the vector for expression (Yu et al., 2009).
  • Detailed Gene Information: Click here.
f. Adjuvant:
  • VO ID: VO_0000142
  • Description: Incomplete Freunds Adjuvant (Sigma)
g. Immunization Route
Intravenous injection (i.v.)
h. Mouse Response
  • Host Strain: C57BL/6
  • Vaccination Protocol: Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein PmpE/F-225–575 in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009).
  • Challenge Protocol: Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009).
  • Efficacy: PmpE/F-2(25-575) immunized mice exhibited significant resistance to challenge infection. In addition, pmpE/F-2 was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
7. C. muridarum PmpG-1 protein vaccine
a. Vaccine Ontology ID:
VO_0011458
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
C. muridarum polymorphic membrane protein G family protein
e. Gene Engineering of PmpG-1
  • Type: Recombinant protein preparation
  • Description: The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: pmpG-1 and gap DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pET32a (Novagen) for pmpG-1 and gap after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques. Only the first half of the gene (25–500) was cloned into the vector for expression (Yu et al., 2009).
  • Detailed Gene Information: Click here.
f. Adjuvant:
  • VO ID: VO_0000142
  • Description: Incomplete Freunds Adjuvant (Sigma)
g. Immunization Route
Intravenous injection (i.v.)
h. Mouse Response
  • Host Strain: C57BL/6
  • Vaccination Protocol: Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein PmpG-1 25–500 in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009).
  • Challenge Protocol: Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009).
  • Efficacy: Overall, vaccination with DCs transfected with PmpG-1(25-500) exhibited the greatest degree of protective immunity among the four Chlamydia Ags tested (Yu et al., 2009).
8. C. muridarum PmpG-1, PmpE/F-2, and MOMP Proteins Vaccine
a. Vaccine Ontology ID:
VO_0004257
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Chlamydia proteins PmpG-1, PmpE/F-2, and MOMP (Yu et al., 2010).
e. Adjuvant:
f. Immunization Route
subcutaneous injection
g. Mouse Response
  • Host Strain: C57BL/6 and BALB/c
  • Vaccination Protocol: Mice were vaccinated 3 times subcutaneously with PmpG-1 plus PmpE/F-2 plus MOMP plus AbISCO-100 (G+F+M+AbISCO) (Yu et al., 2010).
  • Challenge Protocol: Two weeks after the final immunization, the mice in the three animal trials described above were then challenged with live EBs for protection evaluation (Yu et al., 2010).
  • Efficacy: The combination of three Chlamydia proteins formulated with AbISCO exhibited significant protection at day 13 compared to the group with adjuvant alone (Yu et al., 2010).
9. C. muridarum RplF protein vaccine
a. Vaccine Ontology ID:
VO_0011437
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
C. muridarum rplF
e. Gene Engineering of RplF
  • Type: Recombinant protein preparation
  • Description: The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: rplF DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pGEX-6P-3 (GE Healthcare) after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques (Yu et al., 2009).
  • Detailed Gene Information: Click here.
f. Adjuvant:
  • VO ID: VO_0000142
  • Description: Incomplete Freunds Adjuvant (Sigma)
g. Immunization Route
Intravenous injection (i.v.)
h. Mouse Response
  • Host Strain: C57BL/6
  • Vaccination Protocol: Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein RplF in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009).
  • Challenge Protocol: Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009).
  • Efficacy: RplF was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
10. C. muridarum Tarp subunit vaccine
a. Vaccine Ontology ID:
VO_0004202
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
purified C. muridarum Tarp
e. Gene Engineering of Tarp
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant:
g. Adjuvant:
h. Immunization Route
Intramuscular injection (i.m.)
i. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunized intramuscularly with purified C. muridarum Tarp emulsified in CPG-IFA adjuvant or just adjuvant (as a control). Mice were immunized in the hind legs 3 times with an interval of 3 weeks between the first and second injections and 9 days between the second and third injections (Wang et al., 2009).
  • Challenge Protocol: Thirty days after the third immunization, each mouse was inoculated intravaginally with 1 × 10^4 IFUs of live C. muridarum organisms in 20μl of SPG (sucrose-phosphate-glutamate buffer). Five days prior to infection, each mouse was injected with 2.5mg Depo-provera subcutaneously to synchronize menstrual cycle and increase mouse susceptibility to chlamydial infection (Wang et al., 2009).
  • Efficacy: Immunization of mice with Tarp induced Th1-dominant immunity that significantly reduced the shedding of live organisms from the lower genital tract and attenuated inflammatory pathologies in the fallopian tube tissues. Tarp, an immunodominant antigen identified by human antisera, can induce protective immunity against chlamydial infection and pathology in mice (Wang et al., 2009).
V. References
1. Faludi et al., 2009: Faludi I, Burian K, Csanadi A, Miczak A, Lu X, Kakkar VV, Gonczol E, Endresz V. Adjuvant modulation of the immune response of mice against the LcrE protein of Chlamydophila pneumoniae. International journal of medical microbiology : IJMM. 2009; 299(7); 520-528. [PubMed: 19451031].
2. McNeilly et al., 2007: McNeilly CL, Beagley KW, Moore RJ, Haring V, Timms P, Hafner LM. Expression library immunization confers partial protection against Chlamydia muridarum genital infection. Vaccine. 2007; 25(14); 2643-2655. [PubMed: 17239501].
3. Mosolygó et al., 2014: Mosolygó T, Szabó AM, Balogh EP, Faludi I, Virók DP, Endrész V, Samu A, Krenács T, Burián K. Protection promoted by pGP3 or pGP4 against Chlamydia muridarum is mediated by CD4(+) cells in C57BL/6N mice. Vaccine. 2014; 32(40); 5228-5233. [PubMed: 25077421].
4. Skelding et al., 2006: Skelding KA, Hickey DK, Horvat JC, Bao S, Roberts KG, Finnie JM, Hansbro PM, Beagley KW. Comparison of intranasal and transcutaneous immunization for induction of protective immunity against Chlamydia muridarum respiratory tract infection. Vaccine. 2006; 24(3); 355-366. [PubMed: 16153755].
5. Tifrea et al., 2016: Tifrea DF, Barta ML, Pal S, Hefty PS, de la Maza LM. Computational modeling of TC0583 as a putative component of the Chlamydia muridarum V-type ATP synthase complex and assessment of its protective capabilities as a vaccine antigen. Microbes and infection. 2016; 18(4); 245-253. [PubMed: 26706820].
6. Wang et al., 2009: Wang J, Chen L, Chen F, Zhang X, Zhang Y, Baseman J, Perdue S, Yeh IT, Shain R, Holland M, Bailey R, Mabey D, Yu P, Zhong G. A chlamydial type III-secreted effector protein (Tarp) is predominantly recognized by antibodies from humans infected with Chlamydia trachomatis and induces protective immunity against upper genital tract pathologies in mice. Vaccine. 2009; 27(22); 2967-2980. [PubMed: 19428908].
7. Wiki: Chlamydia muridarum: Chlamydia muridarum [http://en.wikipedia.org/wiki/Chlamydia_muridarum]
8. Yu et al., 2009: Yu H, Jiang X, Shen C, Karunakaran KP, Brunham RC. Novel Chlamydia muridarum T cell antigens induce protective immunity against lung and genital tract infection in murine models. Journal of immunology (Baltimore, Md. : 1950). 2009; 182(3); 1602-1608. [PubMed: 19155509].
9. Yu et al., 2009: Yu H, Jiang X, Shen C, Karunakaran KP, Brunham RC. Novel Chlamydia muridarum T cell antigens induce protective immunity against lung and genital tract infection in murine models. Journal of immunology (Baltimore, Md. : 1950). 2009; 182(3); 1602-1608. [PubMed: 19155509].
10. Yu et al., 2010: Yu H, Jiang X, Shen C, Karunakaran KP, Jiang J, Rosin NL, Brunham RC. Chlamydia muridarum T-cell antigens formulated with the adjuvant DDA/TDB induce immunity against infection that correlates with a high frequency of gamma interferon (IFN-gamma)/tumor necrosis factor alpha and IFN-gamma/interleukin-17 double-positive CD4+ T cells. Infection and immunity. 2010; 78(5); 2272-2282. [PubMed: 20231405].
11. Yu et al., 2014: Yu H, Karunakaran KP, Jiang X, Brunham RC. Evaluation of a multisubunit recombinant polymorphic membrane protein and major outer membrane protein T cell vaccine against Chlamydia muridarum genital infection in three strains of mice. Vaccine. 2014; 32(36); 4672-4680. [PubMed: 24992718].