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Chlamydia muridarum |
Table of Contents |
- General Information
- NCBI Taxonomy ID
- Introduction
- Host Ranges and Animal Models
- Vaccine Related Pathogen Genes
- atpA
(Protective antigen)
- MOMP
(Protective antigen)
- pGP4-D
(Protective antigen)
- pmpE
(Protective antigen)
- PmpE/F-2
(Protective antigen)
- PmpG-1
(Protective antigen)
- pmpH
(Protective antigen)
- RplF
(Protective antigen)
- Tarp
(Protective antigen)
- TC0439 adherence factor
(Protective antigen)
- TC0512 outer membrane protein, putative
(Protective antigen)
- TC0757 hypothetical protein
(Protective antigen)
- TC0767 hypothetical protein
(Protective antigen)
- TC0768 hypothetical protein
(Protective antigen)
- Virulence plasmid protein pGP3-D
(Protective antigen)
- Vaccine Related Host Genes
- Ifng (Interferon gamma)
- IgA
- IgG
- Vaccine Information
- C. muridarum DNA vaccine encoding TC0439
- C. muridarum DNA vaccine encoding TC0512
- C. muridarum DNA vaccine encoding TC0757
- C. muridarum DNA vaccine encoding TC0767/768
- C. muridarum MOMP protein vaccine
- C. muridarum PmpE/F-2 protein vaccine
- C. muridarum PmpG-1 protein vaccine
- C. muridarum PmpG-1, PmpE/F-2, and MOMP Proteins Vaccine
- C. muridarum RplF protein vaccine
- C. muridarum Tarp subunit vaccine
- References
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I. General Information |
1. NCBI Taxonomy ID: |
83560 |
2. Introduction |
Chlamydia muridarum is an intracellular bacterial species that at one time belonged to Chlamydia trachomatis. However, C. trachomatis naturally only infects humans and C. muridarum naturally infects only members of the family Muridae (includes both mice and hamsters, Alderton, 1996). Two strains of Chlamydia muridarum, MoPn (originally named Nigg) and SFPD, have been isolated from mice and hamsters. Glycogen production by both strains has been demonstrated, although it is difficult to detect glycogen in MoPn (Moulder et al., 1984). Chlamydia muridarum MoPn binds mAbs recognizing Chlamydia trachomatis MOMP vs4 core epitope (T)LNPT(IA). DNA sequence analysis indicates that these mAbs should recognize SFPD and that Chlamydia trachomatis B-serogroup mAbs specific for the vs4 epitope IAGAG should recognize SFPD (Wiki: Chlamydia muridarum). |
3. Host Ranges and Animal Models |
C. muridarum naturally infects only members of the family Muridae (which includes both mice and hamsters) (Wiki: Chlamydia muridarum). |
II. Vaccine Related Pathogen Genes |
1. atpA |
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Gene Name :
atpA
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Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
-
NCBI Protein GI :
AAF39416
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Other Database IDs :
CDD:235248
-
Taxonomy ID :
243161
-
Protein Name :
ATP synthase, subunit A
-
Protein pI :
4.79
-
Protein Weight :
61088.64
-
Protein Length :
669
-
Protein Note :
V-type ATP synthase subunit A; Provisional
-
Protein Sequence : Show Sequence
>AAF39416.1 ATP synthase, subunit A [Chlamydia muridarum str. Nigg]
MVATSKQTTQGYVVEAYGNLLRVHFDGHVRQGEVAYVSVDDTWLKAEIIEVVGDEVKVQVFEETQGISRG
ALVTFSGHLLEAELGPGLLQGIFDGLQNRLEVLADTSLFLKRGEYVNAICRETVWAYTQKASVGDVLSRG
DVLGTVKEGRFDHKIMVPFSCFEEVTITWVISSGDYTVDTVIAKGRTASGAELEFTMVQKWPIKQAFLEG
EKVPSHEIMDVGLRVLDTQIPVLKGGTFCTPGPFGAGKTVLQHHLSKYAAVDIVVLCACGERAGEVVEIL
QEFPHLTDPHTGQSLMHRTCIICNTSSMPVAARESSIYLGITIAEYYRQMGLHVLLLADSTSRWAQALRE
ISGRLEEIPGEEAFPAYLASRIAAFYERGGAVKMKDGSEGSLTICGAVSPAGGNFEEPVTQATLSVVGAF
CGLSKARADARRYPSIDPMISWSKYLDSVAEILEKKVPGWGDSVKKASRFLEEGAEIGKRIEVVGEEGIS
MEDIEIFLKSELYDFCYLQQNAFDAEDCYCPFDRQIELFSLMSHIFSSRFCFDCPDNARSFFLELQSKIK
TLNGQKFLSEDYQKGLEVIYKLLESKMVQTA
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Tifrea et al., 2016)
|
2. MOMP |
-
Gene Name :
MOMP
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VO ID :
VO_0010890
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NCBI Protein GI :
1518659
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Other Database IDs :
CDD:279628
-
Taxonomy ID :
83560
-
Gene Strand (Orientation) :
?
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Protein Name :
major outer membrane protein
-
Protein pI :
4.86
-
Protein Weight :
39872.84
-
Protein Length :
457
-
Protein Note :
mouse pneumonitis; submitted as Chlamydia trachomatis
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Protein Sequence : Show Sequence
>AAB07068.1 major outer membrane protein [Chlamydia muridarum]
MKKLLKSVLAFAVLGSASSLHALPVGNPAEPSLMIDGILWEGFGGDPCDPCTTWCDAISLRLGYYGDFVF
DRVLKTDVNKQFEMGAAPTGDADLTTAPTPASRENPAYGKHMQDAEMFTNAAYMALNIWDRFDVFCTLGA
TSGYLKGNSAAFNLVGLFGRDETAVAADDIPNVSLSQAVVELYTDTAFAWSVGARAALWECGCATLGASF
QYAQSKPKVEELNVLCNAAEFTINKPKGYVGQEFPLNIKAGTVSATDTKDASIDYHEWQASLALSYRLNM
FTPYIGVKWSRASFDADTIRIAQPKLETSILKMTTWNPTISGSGIDVDTKITDTLQIVSLQLNKMKSRKS
CGLAIGTTIVDADKYAVTVETRLIDERAAHVNAQFRF
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Molecule Role :
Protective antigen
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Molecule Role Annotation :
Female BALB/c mice were immunized with chlamydial major outer membrane protein (MOMP) mixed with cholera toxin and CpG oligodeoxynucleotide adjuvants by either the IN or TCI routes. Both intranasal and transcutaneous immunization protected mice against respiratory challenge with Chlamydia (Skelding et al., 2006).
- Related Vaccine(s):
C. muridarum MOMP protein vaccine
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3. pGP4-D |
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Gene Name :
pGP4-D
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Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
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NCBI Protein GI :
Q46440
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Other Database IDs :
CDD:235774
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Taxonomy ID :
243161
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Protein Name :
Virulence plasmid protein pGP4-D
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Protein pI :
9.61
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Protein Weight :
13082.5
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Protein Length :
176
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Protein Note :
Virulence plasmid protein pGP4-D. /FTId=PRO_0000218345.
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Protein Sequence : Show Sequence
>sp|Q46440.1|GP4D_CHLMU RecName: Full=Virulence plasmid protein pGP4-D
MQNKRKLRNDFIKIVKDVEKDFPELDLKIRVNKERVTFLNSPLELYHKSISLILGLLQQIEKSLELFPDS
PVLEKLEDNSLKLKKALIMLILSRKDMFSKAE
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Molecule Role :
Protective antigen
-
Molecule Role Annotation :
|
4. pmpE |
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Gene Name :
pmpE
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Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
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NCBI Protein GI :
Q9PL47
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Other Database IDs :
CDD:330218
CDD:311484 CDD:214872
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Taxonomy ID :
243161
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Protein Name :
Probable outer membrane protein PmpE
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Protein pI :
6.79
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Protein Weight :
105209.61
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Protein Length :
1130
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Protein Note :
Polymorphic membrane protein E
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Protein Sequence : Show Sequence
>sp|Q9PL47.1|PMPE_CHLMU RecName: Full=Probable outer membrane protein PmpE; AltName: Full=Polymorphic membrane protein E; Flags: Precursor
MKKLFFFVLIGSSILGFTREVPPSILLKPILNPYHMTGLFFPKVNLLGDTHNLTDYHLDNLKCILACLQR
TPYEGAAFTVTDYLGFSDTQKDGIFCFKNLTPESGGVIGSPTQNTPTIKIHNTIGPVLFENNTCHRLWTQ
TDPENEGNKAREGGAIHAGDVYISNNQNLVGFIKNFAYVQGGAISANTFAYKENKSSFLCLNNSCIQTKT
GGKGGAIYVSTSCSFENNNKDLLFIQNSGCAGGAIFSPTCSLIGNQGDIVFYSNHGFKNVDNATNESGDG
GAIKVTTRLDITNNGSQIFFSDNISRNFGGAIHAPCLHLVGNGPTYFTNNIANHTGGAIYITGTETSKIS
ADHHAIIFDNNISANATNADGSSSNTNPPHRNAITMDNSAGGIELGAGKSQNLIFYDPIQVTNAGVTVDF
NKDASQTGCVVFSGATVLSADISQANLQTKTPATLTLSHGLLCIEDRAQLTVNNFTQTGGIVALGNGAVL
SSYQHSTTDATQTPPTTTTTDASVTLNHIGLNLPSILKDGAEMPLLWVEPISTTQGNTTTYTSDTAASFS
LNGATLSLIDEDGNSPYENTDLSRALYAQPMLAISEASDNQLQSESMDFSKVNVPHYGWQGLWTWGWAKT
ENPTTTPPATITDPKKANQFHRTLLLTWLPAGYIPSPKHKSPLIANTLWGNILFATENLKNSSGQELLDR
PFWGITGGGLGMMVYQEPRKDHPGFHMHTSGYSAGMITGNTHTFSLRFSQSYTKLNERYAKNYVSSKNYS
CQGEMLLSLQEGLMLTKLIGLYSYGNHNSHHFYTQGEDLSSQGEFHSQTFGGAVFFDLPLKPFGRTHILT
APFLGAIGMYSKLSSFTEVGAYPRTFITETPLINVLIPIGVKGSFMNATHRPQAWTVELAYQPVLYRQEP
SISTQLLAGKGMWFGHGSPASRHALAYKISQKTQLLRFATLQLQYHGYYSSSTFCNYLNGEVSLRF
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Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Yu et al., 2009)
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5. PmpE/F-2 |
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Gene Name :
PmpE/F-2
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Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
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VO ID :
VO_0010893
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NCBI Gene ID :
1246432
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NCBI Protein GI :
497915819
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Locus Tag :
TC_RS01330
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Genbank Accession :
AE002160
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Protein Accession :
WP_010229975
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Taxonomy ID :
243161
-
Gene Starting Position :
308276
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Gene Ending Position :
311353
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Gene Strand (Orientation) :
-
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Protein Name :
autotransporter domain-containing protein
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Protein pI :
8.26
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Protein Weight :
107782.38
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Protein Length :
1025
-
Protein Note :
similar to GP:4376753; identified by sequence similarity; putative
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DNA Sequence : Show Sequence
>NC_002620.2:308276-311353 Chlamydia muridarum Nigg, complete genome
GTTAAAAGACCAGAGCCCCTCCTGCATTCATGTAGTGTGAGACTGTAGAAGTAGCCACCTGCGCTTGATA
ATTAGCGAATAGTTTAAGATAAGAGAATTTTAGAGAAGATGATCCTCTCCCATAAAAGGAATGCTTAGCT
AAAGGAGTGTTTGTTGTTTCCCAAGAACCATTGTTTTTGATTAAAACGGTATTAAGAATGGGGCGCTTCC
AGTAAAGAGTAGGCTGGTATGCTACCTCCATTTCGCAGGATACAGTTGGCCATTTATTCGAAGAATAAAC
ACCTTTAATTCCTATTGGAGAAACAACAGCTGTATGTGGTTGTTTTAACGAAAATAATCTAGCCAGGTCT
CCCTGCTCCTGGATAGATGCAGGCTCTGTTCGGGAGATGAGAGCTTGAATGAATGGTGTCAACATAATCG
ATTGGAAAGGCATACTATCCCGCAGTTCGCAACGTAGAGAGCCCCCCAGAGTCGTAGAGTGAAAGTTCCC
TTTAGAAAACTTTTTCGTTCCATAGAAGTTATATGTTTTATGAGTACCATAGTTATAAGCAGCTGCTCCA
ATAAGAGCGATACGATCAAACATAGGTTGTTCAAAACACAGAGCGGAGAGATAATAACGAGAAGAAATTT
TATTATTGGTACGTTTCTCTTTCATTGTGTCGGAGGATTGGGAAAAGGAAAATAAAAACTTATGTCCGTG
TGCTCCTGATGCTTGTGAAGATATAGCATATCCTTTCGATGCAGAAGAATATCCGTGATAATCTAAGCGT
TTCTGCTGTTTGGTGAATAGCGTAGCTGCAGATCCTTGTAAAGCGAACCAAGAGTCATCTGGTAGTAATG
AACGTAAGTACAGGGTTGTATTTCTTCCAGAAGACACTAAATTATTCGCAATAAGATCCCCTCTACGAGC
AGGATCTGCTATATATCCGATTGGGGTCCAGCTCACTACTAAGGAGTGTCTAGTGACTGTTACGTCCGTT
GTAGCAAGAGGAGTTGTTCCTCCTGTAGAAGCTCTAATGGAAGGAATCTTAGCTGTAGTTGTACTGTTGG
AATCAAAAGTTTCGCTAGTGTTTTTTTGTTCTTGAATAGAATTGTTGGCTGTTTGAGTCTCTGTACTATT
GGTAAGAGAGACCGTACGGGTGGTTGTCATCCAATTAGATGTCCAGAGTCCTTGGTATCCGTAATGTGGT
AAAGAATATAGCGAGGTATTGAGAGTGTTTTTCCAATCATTGCTTAACTGCGCATCATTAGTCGTTTCGA
TATGTAAAATGGGTTGATTTGCTTTTCCTCGGGATAGGTCGCAGCTGTCATAAACTGATTCGTAATTTTC
ATCTACTAACGAGACAAGACCGGAGAAAAAGATCTTTTGAGTTGCCACGTTCTCTGTGCTTACTGGAGCT
TGGTTAGAGTTTGTATTCTGTGTCATATCTTGGGGATTAACACGAATACGTGGAGCTGTAGTACCTTGGT
CATTGAGAAGACCTGGCAAAATGATTCCAAGATGCGTAATAGTGAGAGAAGCGTCTTTCCCTACAGTAAG
AATAGAATCTTGTCCTAAAGCAAGAAGGCTAGTGCTTTCTTGAGTAAGAGGGCCATTGAAAAATTCAAAC
TCTGCGCCATTTTCTATGGACATAGTCCCAAACTTCAAGGTAGTGGGGGAGTCTTTAATTTGGTTATGAG
TTTTACTGGCAATCATGTGAGCTTGTAGATCACTGGATATGTTTTTGTATGAAAACACAACAGCGCCAGA
GAACTCATCCGAAGGATTGATGGTAACGGTGTGCGGGCTATTTTCAGGGGGTGTATTAGCTTTTTTTAAG
CCTCCCCATTGAAAAAGATCATAAAAATAGATGCGAGTATCTTTCTTAGCACCAAGAGATAGGGTGATAG
GGTTATTGTTGGTTGTTCCAGGAATATAAAAAAGTTTTCGCAAACCTGCTGACATTCCGGTAGTTGAAAG
CTCTGTATTATTGTTGAAGATAATATCCCCAGAGCCAGTATGAATATAGTTCGCAGCTGGGCTTGCAAAT
AGGTGAAAAGCTCCTCCATACAGAGCCGAATTATTAAGAAAATAGATAGGACCATTTTCTTGAAAATTAA
GGTTGTTGCAGGAAATAGCTCCTGCTATGTATGAGGCATTATTATTCTCAAAGTAGATAGGGCCTTTATT
ATTTAGAAGACCAAACTCTCCTCCTGCGCTTATAACTGCTCCATTTCCTTGAGCACTAGTGTTATGCCAT
CCTAAAGCGCTATTATTTATAAAAATAAGCTTATCCTTATTATCTCTAAAAATACACTGTCCCCCGGTGC
TGATAGCTCCTCCGTATCCTCCATAGTTGGAATTGAAGACTACCGGACCAGTATTGTGTGAGATTTCGCA
GGCACCTTGGCAGCCAACAGCTCCTCCTCGAGCAAGAATTGTAGCAAATGTGTTGGGTATGTGATCAGTA
GTATGGTTATTTTCAAAAGCAACTATTCCGGACGTATTTTTGATAACTAGGTTTTGGATTGTGGATACGG
CTCCTCCCATATCCCCAGAAGTGTTATTGATAAAATTTAAGCTATGATTATTAATTAGCTCGATGCTTGA
TCCGTATAAAGCCCCTCCGCGATTCCTTTCGGTTCTTAATGCTACTCCTGTAAATTGAGTTCCAATAGTT
GTATTATTATAGAACAATATGTTTTGGTTGTTTTTAAAGCTAATAAGGCCTGTAGCATTTATAGCTCCCC
CTCCTCCCTGATGGGTGCAATAATTGTTACAAAAAAACAAGTCTGCATTGTCAATGGCTGTGAAAGAAGT
CATAGACTCAACAAAAGCTCCACTAGAGTTTGTAGTGCTTAAACCTCTCGAAAACCATTGATCCTGCGTA
CAACTTGAAATAGTAAAGTTCCCTCTATAATTAGAAACATTTGCGTAAGTGATGTATTGAGCAGCAATGG
AGCTAGGCGGAGTAACTATGAATTCAATCTCTCTACCACCAAAAGTCAAGTTCGGAAGTTTGAGTGTATC
GGTTTCACTGGCCGAAATACAAGATAAAGGAATGAAAACAAGTGAAAGAGGGAGAATTCTGCGAGTCA
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Protein Sequence : Show Sequence
>WP_010229975.1 autotransporter domain-containing protein [Chlamydia muridarum]
MTRRILPLSLVFIPLSCISASETDTLKLPNLTFGGREIEFIVTPPSSIAAQYITYANVSNYRGNFTISSC
TQDQWFSRGLSTTNSSGAFVESMTSFTAIDNADLFFCNNYCTHQGGGGAINATGLISFKNNQNILFYNNT
TIGTQFTGVALRTERNRGGALYGSSIELINNHSLNFINNTSGDMGGAVSTIQNLVIKNTSGIVAFENNHT
TDHIPNTFATILARGGAVGCQGACEISHNTGPVVFNSNYGGYGGAISTGGQCIFRDNKDKLIFINNSALG
WHNTSAQGNGAVISAGGEFGLLNNKGPIYFENNNASYIAGAISCNNLNFQENGPIYFLNNSALYGGAFHL
FASPAANYIHTGSGDIIFNNNTELSTTGMSAGLRKLFYIPGTTNNNPITLSLGAKKDTRIYFYDLFQWGG
LKKANTPPENSPHTVTINPSDEFSGAVVFSYKNISSDLQAHMIASKTHNQIKDSPTTLKFGTMSIENGAE
FEFFNGPLTQESTSLLALGQDSILTVGKDASLTITHLGIILPGLLNDQGTTAPRIRVNPQDMTQNTNSNQ
APVSTENVATQKIFFSGLVSLVDENYESVYDSCDLSRGKANQPILHIETTNDAQLSNDWKNTLNTSLYSL
PHYGYQGLWTSNWMTTTRTVSLTNSTETQTANNSIQEQKNTSETFDSNSTTTAKIPSIRASTGGTTPLAT
TDVTVTRHSLVVSWTPIGYIADPARRGDLIANNLVSSGRNTTLYLRSLLPDDSWFALQGSAATLFTKQQK
RLDYHGYSSASKGYAISSQASGAHGHKFLFSFSQSSDTMKEKRTNNKISSRYYLSALCFEQPMFDRIALI
GAAAYNYGTHKTYNFYGTKKFSKGNFHSTTLGGSLRCELRDSMPFQSIMLTPFIQALISRTEPASIQEQG
DLARLFSLKQPHTAVVSPIGIKGVYSSNKWPTVSCEMEVAYQPTLYWKRPILNTVLIKNNGSWETTNTPL
AKHSFYGRGSSSLKFSYLKLFANYQAQVATSTVSHYMNAGGALVF
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Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. In addition, pmpE/F-2 was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
- Related Vaccine(s):
C. muridarum PmpE/F-2 protein vaccine
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6. PmpG-1 |
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Gene Name :
PmpG-1
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Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
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VO ID :
VO_0010891
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NCBI Gene ID :
1246433
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NCBI Protein GI :
497915821
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Locus Tag :
TC_RS01335
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Genbank Accession :
AE002160
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Protein Accession :
WP_010229977
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Taxonomy ID :
243161
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Gene Starting Position :
311528
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Gene Ending Position :
314488
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Gene Strand (Orientation) :
+
-
Protein Name :
Pmp family polymorphic membrane protein autotransporter adhesin
-
Protein pI :
5.8
-
Protein Weight :
98285.26
-
Protein Length :
986
-
Protein Note :
similar to GP:4376736; identified by sequence similarity; putative
-
DNA Sequence : Show Sequence
>NC_002620.2:311528-314488 Chlamydia muridarum Nigg, complete genome
GATGCAAACGCCTTTTCATAAGTTCTTTCTTCTAGCAATGCTATCTTACTCTTTATTGCAAGGAGGGCAT
GCGGCAGATATTTCCATGCCTCCGGGAATTTATGATGGGACAACATTGACGGCGCCATTTCCCTACACTG
TGATCGGAGATCCCAGAGGGACAAAGGTTACTTCATCGGGATCGCTAGAGTTGAAAAACCTGGACAATTC
CATTGCGACTTTACCTCTAAGTTGTTTTGGTAATTTGTTGGGGAATTTCACTATTGCAGGAAGAGGGCAT
TCGTTAGTATTTGAGAATATACGAACATCTACAAATGGGGCGGCATTGAGTAATCATGCTCCTTCTGGAC
TGTTTGTAATTGAAGCTTTTGATGAACTCTCTCTTTTGAATTGTAATTCATTGGTATCTGTAGTTCCTCA
AACAGGGGGTACGACTACTTCTGTTCCTTCTAATGGGACGATCTATTCTAGAACAGATCTTGTTCTAAGA
GATATCAAGAAGGTTTCTTTCTATAGTAACTTAGTTTCTGGAGATGGGGGAGCTATAGATGCACAAAGTT
TAATGGTTAACGGAATTGAAAAACTTTGTACCTTCCAAGAAAATGTAGCGCAGTCCGATGGGGGAGCGTG
TCAGGTAACAAAGACCTTCTCTGCTGTGGGCAATAAGGTTCCTTTGTCTTTTTTAGGCAATGTTGCTGGT
AATAAGGGGGGAGGAGTTGCTGCTGTCAAAGATGGTCAGGGGGCAGGAGGGGCGACTGATCTATCGGTTA
ATTTTGCCAATAATACTGCTGTAGAATTTGAGGGAAATAGTGCTCGAATAGGTGGAGGGATCTACTCGGA
CGGAAATATTTCCTTTTTAGGGAATGCAAAGACAGTTTTCCTAAGTAACGTAGCTTCGCCTATTTATGTT
GACCCTGCTGCTGCAGGAGGACAGCCCCCTGCAGATAAAGATAACTATGGAGATGGAGGAGCCATCTTCT
GCAAAAATGATACTAACATAGGTGAAGTCTCTTTCAAAGACGAGGGTGTTGTTTTCTTTAGTAAAAATAT
TGCCGCAGGAAAGGGGGGCGCTATTTATGCTAAGAAACTGACAATTTCTGACTGTGGTCCGGTCCAGTTT
CTTGGTAATGTCGCGAATGACGGGGGCGCTATTTATCTAGTAGATCAGGGGGAACTTAGTCTATCTGCTG
ATCGCGGAGATATTATTTTTGATGGAAATTTAAAGAGAATGGCTACGCAAGGCGCTGCCACCGTCCATGA
TGTAATGGTTGCATCGAATGCTATCTCTATGGCTACAGGGGGGCAAATCACAACATTAAGGGCTAAGGAA
GGTCGCCGAATTCTTTTTAATGACCCTATTGAAATGGCGAATGGACAACCTGTAATACAAACTCTTACAG
TAAACGAGGGCGAAGGATATACGGGGGACATTGTTTTTGCTAAAGGTGATAATGTTTTGTACTCAAGTAT
TGAGCTGAGTCAGGGAAGAATTATTCTCCGAGAGCAAACAAAATTATTGGTTAACTCCCTGACTCAGACT
GGAGGGAGTGTACATATGGAAGGGGGGAGTACACTAGACTTTGCAGTAACAACGCCACCAGCTGCTAATT
CGATGGCTCTTACTAATGTACACTTCTCCTTAGCTTCTTTACTAAAAAATAATGGGGTTACAAATCCTCC
AACGAATCCTCCAGTACAGGTTTCTAGTCCAGCTGTAATTGGTAATACAGCTGCTGGTACTGTTACGATT
TCTGGTCCGATCTTTTTTGAAGATTTAGATGAAACTGCTTACGATAATAATCAGTGGTTAGGTGCGGATC
AAACTATTGATGTGCTGCAGTTGCATTTAGGAGCGAATCCTCCGGCTAACGCTCCAACTGATTTGACTTT
AGGGAACGAAAGTTCTAAATATGGGTATCAAGGAAGTTGGACACTTCAATGGGAACCAGATCCTGCGAAT
CCTCCACAGAACAATAGCTACATGTTGAAGGCAAGCTGGACTAAAACAGGTTATAATCCTGGTCCGGAGC
GCGTAGCTTCTCTGGTCTCTAATAGTCTTTGGGGATCCATTTTAGATGTGCGTTCCGCGCATTCTGCGAT
TCAAGCAAGTATAGATGGACGAGCTTATTGTCGGGGTATTTGGATTTCTGGGATTTCGAACTTTTTCTAT
CATGATCAGGATGCTTTAGGACAGGGGTATCGTCATATTAGTGGGGGATATTCGATAGGAGCAAACTCTT
ATTTCGGGTCTTCTATGTTTGGACTTGCTTTTACTGAAACTTTTGGTAGGTCCAAAGATTATGTGGTCTG
TCGATCTAACGATCACACTTGTGTAGGCTCTGTTTACTTATCCACTAGACAAGCGTTATGCGGATCCTGT
TTATTTGGAGATGCTTTTGTTCGGGCGAGTTACGGATTTGGAAATCAGCATATGAAGACCTCTTATACAT
TTGCTGAAGAGAGTAATGTGCGTTGGGATAATAACTGTGTAGTGGGAGAAGTTGGAGCTGGGCTCCCTAT
CATGCTCGCTGCATCTAAGCTTTATCTAAATGAGTTGCGTCCCTTCGTGCAAGCAGAGTTTGCTTATGCA
GAGCATGAATCTTTTACAGAGAGAGGGGATCAGGCTAGGGAGTTTAAGAGTGGGCATCTTATGAATCTAT
CTATTCCAGTTGGGGTGAAGTTTGATCGATGCTCTAGTAAACATCCTAACAAGTATAGTTTTATGGGAGC
TTATATCTGTGATGCTTACCGGTCCATTTCTGGAACGGAGACAACACTCCTGTCTCATAAAGAGACTTGG
ACAACAGATGCTTTCCATTTAGCAAGGCATGGAGTTATGGTCAGAGGATCTATGTATGCTTCTTTAACAG
GTAATATAGAAGTCTATGGCCATGGAAAATATGAATACAGGGATGCCTCTCGAGGGTATGGTTTAAGTAT
TGGAAGTAAAATCCGATTCTA
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Protein Sequence : Show Sequence
>WP_010229977.1 autotransporter domain-containing protein [Chlamydia muridarum]
MQTPFHKFFLLAMLSYSLLQGGHAADISMPPGIYDGTTLTAPFPYTVIGDPRGTKVTSSGSLELKNLDNS
IATLPLSCFGNLLGNFTIAGRGHSLVFENIRTSTNGAALSNHAPSGLFVIEAFDELSLLNCNSLVSVVPQ
TGGTTTSVPSNGTIYSRTDLVLRDIKKVSFYSNLVSGDGGAIDAQSLMVNGIEKLCTFQENVAQSDGGAC
QVTKTFSAVGNKVPLSFLGNVAGNKGGGVAAVKDGQGAGGATDLSVNFANNTAVEFEGNSARIGGGIYSD
GNISFLGNAKTVFLSNVASPIYVDPAAAGGQPPADKDNYGDGGAIFCKNDTNIGEVSFKDEGVVFFSKNI
AAGKGGAIYAKKLTISDCGPVQFLGNVANDGGAIYLVDQGELSLSADRGDIIFDGNLKRMATQGAATVHD
VMVASNAISMATGGQITTLRAKEGRRILFNDPIEMANGQPVIQTLTVNEGEGYTGDIVFAKGDNVLYSSI
ELSQGRIILREQTKLLVNSLTQTGGSVHMEGGSTLDFAVTTPPAANSMALTNVHFSLASLLKNNGVTNPP
TNPPVQVSSPAVIGNTAAGTVTISGPIFFEDLDETAYDNNQWLGADQTIDVLQLHLGANPPANAPTDLTL
GNESSKYGYQGSWTLQWEPDPANPPQNNSYMLKASWTKTGYNPGPERVASLVSNSLWGSILDVRSAHSAI
QASIDGRAYCRGIWISGISNFFYHDQDALGQGYRHISGGYSIGANSYFGSSMFGLAFTETFGRSKDYVVC
RSNDHTCVGSVYLSTRQALCGSCLFGDAFVRASYGFGNQHMKTSYTFAEESNVRWDNNCVVGEVGAGLPI
MLAASKLYLNELRPFVQAEFAYAEHESFTERGDQAREFKSGHLMNLSIPVGVKFDRCSSKHPNKYSFMGA
YICDAYRSISGTETTLLSHKETWTTDAFHLARHGVMVRGSMYASLTGNIEVYGHGKYEYRDASRGYGLSI
GSKIRF
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Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. Overall, vaccination with DCs transfected with PmpG-1(25-500) exhibited the greatest degree of protective immunity among the four Chlamydia Ags tested (Yu et al., 2009).
- Related Vaccine(s):
C. muridarum PmpG-1 protein vaccine
|
7. pmpH |
-
Gene Name :
pmpH
-
Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
-
NCBI Protein GI :
Q9PL44
-
Other Database IDs :
CDD:273587
CDD:311484 CDD:214872
-
Taxonomy ID :
243161
-
Protein Name :
Probable outer membrane protein PmpH
-
Protein pI :
7.11
-
Protein Weight :
100297.73
-
Protein Length :
1134
-
Protein Note :
Polymorphic membrane protein H
-
Protein Sequence : Show Sequence
>sp|Q9PL44.2|PMPH_CHLMU RecName: Full=Probable outer membrane protein PmpH; AltName: Full=Polymorphic membrane protein H; Flags: Precursor
MPFSLRSTSFCFLACLCSYSYGLASSPQVLTPNVIIPFKGDDIYLNGDCVFASIYAGAEQGSIISANGQN
LTIVGQNHTLSFTDSQGPALQNCAFISAEEKISLRDFSSLLFSKNVSCGEKGMISGKTVSISGGDSIVFK
DNSVGYSSLPSVGQTPTTPIVGDVLKGSIFCVETGLEISGVKKELVFDNTAGNFGAVFCSRAAQGDTTFT
VKDCKGKILFQDNVGSCGGGVIYKGEVLFQDNEGEMLFRGNSAHDDLGILDANPQPPTEVGGGGGVICTP
EKTVTFKGNKGPITFDYNFAKGRGGAIQSQTFSLVADSAVVFSNNTAEKGGGAIYALEVNVSTNGGSILF
EGNRASEGGAICVSEPIAANNGGLTLHAADGDIIFSKNMTSDRPGERSAIRILDSGTNVSLNASGASKMI
FYDPVVQNNPATPPTGTSGEIKINESGSGSVVFTAETLTPSEKLNVINATSNFPGNLTVSSGELVVTKGA
TLTVGNITATSGRVTLGSGASLSAVAGTAGTCTVSKLGIDLESFLVPTYETAKLGADTTVAVNNNPTLDL
VMANETEMYDNPLFMNAVTIPFVTLVSLQTTGGVTTSAVTLNNADTAHYGYQGSWSADWRRPPLAPDPSG
MTPLDKSNTLYVTWRPSSNYGVYKLDPQRRGELVPNSLWVSGSALRTFTNGLKEHYVSRDVGFIASVQAL
GDYVLNYKQGNRDGFLARYGGFQAVAASHYENGGIFGVAFGQLYGQTKSRLYDSKDAGNITILSCFGRSY
IDVKGTETVVYWETAYGYSVHRMHTQYFNGKTNKFDHSKCRWHNNSYYAFVGAEHNFLEYCIPTRQLARD
YDLTGFMRFEMSGGWSSGAKETGALPRHFDRGTGHNMSLPIGVVAHAVSNGRRSPPSKLTINMGYRPDIW
RVTPHCNMKIIANGVKTPIQGSPLARHAFFLEVHDTLYVRHLGRAYMNYSLDARHRQTTHFVSLGLNRIF
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Yu et al., 2014)
|
8. RplF |
-
Gene Name :
RplF
-
Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
-
VO ID :
VO_0010892
-
NCBI Gene ID :
1246168
-
NCBI Protein GI :
497917443
-
Locus Tag :
TC_RS04070
-
Genbank Accession :
AE002160
-
Protein Accession :
WP_010231599
-
Taxonomy ID :
243161
-
Gene Starting Position :
941594
-
Gene Ending Position :
942145
-
Gene Strand (Orientation) :
-
-
Protein Name :
50S ribosomal protein L6
-
Protein pI :
10.64
-
Protein Weight :
18060.75
-
Protein Length :
183
-
Protein Note :
ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance
-
DNA Sequence : Show Sequence
>NC_002620.2:941594-942145 Chlamydia muridarum Nigg, complete genome
TCTATTTTTTACCAGTTTTCGCTGCTTTTCCAGCCTTACGACGTACATATTCGTTTTCGTAACGAATTCC
TTTACCTTTGTATGGCTCTGGAGGGCGTTTTGCACGAACACAAGCCGCAAATTCTCCTACCAACTGCTTG
TTGATACCTTTGATGGAGATCAATGTGTTCTTCTCAACAGAGACTTCCAATCCTGTAGGAATAGGCATTT
TTGTTGGATGGGAAACCCCTATCGATAAATCTAAGAAGGACCCCTGTACCGAAGCTCTGAATCCAACTCC
GATCATTTCTAAACGTTTTTCAAATCCAAGGTGGACACCTTTGACCATATTTGCTATCAAAGCCCAATAA
AGCCCTTGCATACGACCAGGTCTGTCTATAATGTGAGCTGCGGGAGAAACAAACACCTCATTACCTTTAA
CGGCAATCTCAACTTCTTTAGCCAACACCTGTGTCAAAGACCCTTTAGGACCTTTTACTGAGATTTCATC
ATTTTGAATAGAGACCTCTACGCCTTGAGGAAGTACAATAGGGTCTCGAGCTTTACGAGACA
-
Protein Sequence : Show Sequence
>WP_010231599.1 50S ribosomal protein L6 [Chlamydia muridarum]
MSRKARDPIVLPQGVEVSIQNDEISVKGPKGSLTQVLAKEVEIAVKGNEVFVSPAAHIIDRPGRMQGLYW
ALIANMVKGVHLGFEKRLEMIGVGFRASVQGSFLDLSIGVSHPTKMPIPTGLEVSVEKNTLISIKGINKQ
LVGEFAACVRAKRPPEPYKGKGIRYENEYVRRKAGKAAKTGKK
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. In addition, RplF was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
- Related Vaccine(s):
C. muridarum RplF protein vaccine
|
9. Tarp |
-
Gene Name :
Tarp
-
Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
-
NCBI Gene ID :
1245826
-
NCBI Protein GI :
497916366
-
Locus Tag :
TC_RS02355
-
Genbank Accession :
AE002160
-
Protein Accession :
WP_010230522
-
Taxonomy ID :
243161
-
Gene Starting Position :
571045
-
Gene Ending Position :
572607
-
Gene Strand (Orientation) :
+
-
Protein Name :
peptide ABC transporter substrate-binding protein
-
Protein pI :
9.01
-
Protein Weight :
56833.03
-
Protein Length :
520
-
Protein Note :
similar to GB:D00760, SP:P25787, and PID:220024; identified by sequence similarity; putative
-
DNA Sequence : Show Sequence
>NC_002620.2:571045-572607 Chlamydia muridarum Nigg, complete genome
CATGCGCAAGGTATCAGTGGGAATCTGCTTGCTTATCGCATTAGCAACAGCAATTACCGGCTGCTCCAAA
TCCTCCTCAAACAAATCAAATCACTCCTCCTCTAATCAAAGCGTATCTGTGAGCATGAAGGACGATCCTC
GCACGTTTGACCCTCGAGAGGTTAGGCTTCTTTCGGATATTAATTTGATTCACCATCTTTATGAGGGACT
GGTTCAAGAGACTCCTTCCGGAGAGGTTTTCCCTGCATTAGCAGAAAGTTTCTTCCTATCTGAAGATAAG
AAAACCTACACTTTCCACCTAAAAAAAGCACTCTGGAGTAATGGAGACCTGATTACTGCTCATGATTTTG
TACGTTCTTGGAACGATGTTTTGCAAAATCGCATTGCAAGTATCTACTCTTTCGCTTTTTTACCCATTGA
TTTAAGTAAAGATTGTGGATTCTTCGCTAAGGACAATCATACACTTGTTATCAATTTGCATACCCCTACT
CCACACTTTCTGAAATTACTTACCCTTCCAGTATTTTATCCTGTGCACCCCGAACATCAAATTCGGAATG
AAGCAAAAGCTCTTCCAATATCTACAGGGGCTTTTTGTTTAAAAGAGAAAAAAGATCGGCGGTGGCTTAA
ATTAGAAAAAAATCCCTATTATTATAACAAAGAGCAAGTTGCGATCCAGGAAATCCACATACATGTGATT
CCTGATCAGCAGACCGCTTCTGCTTTGTTCAAACAAGGAAAATTGGATTGGCAAGGACTTCCTTGGGGAC
ACAGCATTCCCCAAGAAGCTTTAGCAACAGCAAATAAACGTCGTACTCCTCAATCATTTGATATATCTGG
CACCTCGTGGCTCACGTTCAACACATCAAAAGTACCCTTCAGCCATCCCAAGTTGCGACAAGCTTTAAGC
CTTGTTCTAAATAAGGAAGCCTTAGCCTCTCCAACCTTTGTTAAGCCCGCTAAACACCTACTTCCTACAC
ACCTGCACACTTATCCAGAGCAACCTACTTACAAACAACAAGAAGCCGTCATTTTAGCCAAAACTCTGTT
ACAAGAAGCATTAACAGATCTTAATATGACTATAAAAGATCTTGAAAAATGCCCTTTGATTTTTTCAGCG
ACATCTAGCGTCAACTCACAAATGGCCCAAATGATGCGCGATCAATGGCGAAGAATTTTAGGAATAACAT
TCCCTATTTGCGGGAAAGAGTATGCTTTATTACAAAATGATTTGACCACCAACACTTTTTTTATGTCCAT
AAACGGTTGGTTTGCAGACTTTTCCGATCCTTTAGCCTTCTTGTCTGTTTTTTCTTCTAAGGGAATCAAA
CCTTATGCCTTACAAGACCCTCTGTTTGACCAGCTTATTCTCTCTATAGAAACGGAAAAAGATCCTCGAA
AGCGTATTTCTCTGATTTCCGAAGCTTCTCTATACCTAGAAAAACAAAATATCATAGAACCGCTCTATCA
CGATGTTTTTCACTATGCCGCAAATAATAAACTTTCTTTTGTTAGATTACATCCCTCAGGTCTTGTCGAT
ATGCGGTATGCAAAAAACTCTTG
-
Protein Sequence : Show Sequence
>WP_010230522.1 peptide ABC transporter substrate-binding protein [Chlamydia muridarum]
MRKVSVGICLLIALATAITGCSKSSSNKSNHSSSNQSVSVSMKDDPRTFDPREVRLLSDINLIHHLYEGL
VQETPSGEVFPALAESFFLSEDKKTYTFHLKKALWSNGDLITAHDFVRSWNDVLQNRIASIYSFAFLPID
LSKDCGFFAKDNHTLVINLHTPTPHFLKLLTLPVFYPVHPEHQIRNEAKALPISTGAFCLKEKKDRRWLK
LEKNPYYYNKEQVAIQEIHIHVIPDQQTASALFKQGKLDWQGLPWGHSIPQEALATANKRRTPQSFDISG
TSWLTFNTSKVPFSHPKLRQALSLVLNKEALASPTFVKPAKHLLPTHLHTYPEQPTYKQQEAVILAKTLL
QEALTDLNMTIKDLEKCPLIFSATSSVNSQMAQMMRDQWRRILGITFPICGKEYALLQNDLTTNTFFMSI
NGWFADFSDPLAFLSVFSSKGIKPYALQDPLFDQLILSIETEKDPRKRISLISEASLYLEKQNIIEPLYH
DVFHYAANNKLSFVRLHPSGLVDMRYAKNS
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Immunization of mice with Tarp induced Th1-dominant immunity that significantly reduced the shedding of live organisms from the lower genital tract and attenuated inflammatory pathologies in the fallopian tube tissues. Tarp, an immunodominant antigen identified by human antisera, can induce protective immunity against chlamydial infection and pathology in mice (Wang et al., 2009).
- Related Vaccine(s):
C. muridarum Tarp subunit vaccine
|
10. TC0439 adherence factor |
-
Gene Name :
TC0439 adherence factor
-
Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
-
VO ID :
VO_0010887
-
NCBI Gene ID :
1245792
-
NCBI Protein GI :
497916297
-
Locus Tag :
TC_RS02190
-
Genbank Accession :
AE002160
-
Protein Accession :
WP_010230453
-
Taxonomy ID :
243161
-
Gene Starting Position :
526719
-
Gene Ending Position :
536375
-
Gene Strand (Orientation) :
+
-
Protein Name :
adherence factor
-
Protein pI :
7.34
-
Protein Weight :
344190.12
-
Protein Length :
3218
-
Protein Note :
similar to GP:6013469; identified by sequence similarity; putative
-
DNA Sequence : Show Sequence
>NC_002620.2:526719-536375 Chlamydia muridarum Nigg, complete genome
TATGACTCTTTCGAATACAGCCTCTTCCACAGTTTCACCTAAGTCTACGCAACTTAACCATACTTTATCC
AATCAAACTCACCCTGTTGCTCAAAGCAATAGGAATATAGATTTCCAAATCTATGATGAACAGATTCATA
ATAATCATTCTACAGAAGATGTAGTTGCTATAGGACGTCGTATTCAGCAAGAATATGCGAATCTTACCAC
ATCAAAACAGGTTAATTTCTCGGCATCTCCATCTAACCATACTGGAAACTGGAAGATTTCTTTGCTTTAT
AATTTATCGATGCTGGTTGCAAATCTATTCCCTACAACTATTCAGCCTGTGCAACCTCAAAAAGTCATCT
TTAAGAATCAAGACTCTTCTAAGGAAACCCAACTGACCAAATCATCCAAAGTAGTTTCTTCTCGCTCTTC
TTTAAAAGAGATTTTTTCGAAGAAATCTCCTCCTTTGAGAAAGTCTTTTTCTTCCTCTAAAATTTCTAGA
GCAATCCCTAGAAGAAAAAAACGATCAAACGATGATCCAGTTCAAGCCACAAATACTTATGATTTGACCG
CTGAAAATATTCTTGAAAAACTCTCTTTAACACAAGAACAACAAATTAAGCACGATAATCTGATTAGCAA
TCTCAAAGAAGCAATTAATCGATATAGTGATCTTAATCGGAAAAATTCCCGGAAAGGACAATCCCTTCTT
GTTAGGCAAGCCAAAATTCTAGATGAAATTCTTTCACAGACTAAATCTACAGAGGAACGAGCTTCCAATT
CTGTAATGACGACTATTAAGAAGGAATTTACCTCCCACCGCGTACCTGTAGAGAAAAATATTCACGGAAT
TTGGATTGCAGGTTCACCGCCAGAAGGGACCGATGAATATATTAAATTGTTCCTTCACACTTATCCAGAG
TTTTCCTTCCTTTTTTGGGTGGATAAAACAGCTTATGGCGCAGCAAAATTCTCTTCTACTCTTAAACGAA
TTGCTTTTGATGCAGCAGTTAATTCCTTACGAGAGGCTACTCCAGAGCCTGTCAAGCAGTTTGTTCAACG
TTATGATAAGCTAAAAAAATCATACGATACTTCGCGAGATTTTGATGAAAAACAGCGGTTATCTGAGCAG
TTAGTTGAGCTATACGATAATTACAATAAATTTAGTAAGGAGATTCAAAGTAACTTTGATGTTTTGCTAT
TGCATGAAATGATCACTATTCAAGATAGCTTTTTTAACTACTGCCAGCTCAAAGGAGTAGGTGCAATAAC
AGATGAGACACGTATAGAGTATCTTGAGAAAGTTTTGAAAGTAAAAGAAGAGGATCTATCTCACTATAAA
GAAACTATCAAACGCAACAAAGAGTCTATTGAAAAACTTGTAAAAGAGATTAATGACTCTACTGGACGGG
AGAGAGTAGTTATCAAAGACATCCGAGATTTGAAGTCTTTACAAGATCTTACTAATAGCTACAACTATGA
AACAGAAATGTTACTCCGATGGAATTATGCAGCGGCTACCGACCAGCTTCGTATGTACATGCTTAAAGAA
TATGGGGGGATTTATACAGACCTAGATATTATGCCACAATACTCTCAGGATGTTTTGCAAAAAATTATGG
ATGTTGGTGGGAGTAGGTTTTTTGAGCATGATAAGTTACGGCGGACTCTTTCTTTTGCAGCTCTAAAATT
AGGAAGCGGTAAGCAAACAACTGTTTCCTTTGAAGAGGCTAAAAAAGCTATGACTCTCCCCACATTTACT
CTTCAGGATAAGAGTCAAATTTCTGAAATTTTTAAATATCTAGAAACGGAAACGCAAGCAAAAAAATCAC
TTTTCCAACCGATGGATGTTACCGTCGTCCGTGATTTCATGCCTATTTTACAACGTTATCATAAGTGGCA
AACAGGATGGAATGTTCGGGGATTAAATGGTTTAATGATGGCTCATAAAGATAGTGCTGTTGTCGATGCT
GTTATTGCTCGCCAGCGAGCGGCCTATGATGAGATGCGTGCACTTCGTCAGAATGTTGTTAGTGGAGAAT
TTTTTCGTTCTCTTGGAGATCTTGAACATGTAAATCGTGAAAAGAATATCGGCGGCTATTTAGCGAAAAA
TTATCTTGGGGGCAGTCTTTTTTTCGATTTTCGTCAAGATTCAGTGATTCCAGGAGCTATTAGTACCTTA
GGAATTTCAGGTCCCGATATCATCATGGATACCATGAGCGATTATTTTACAAATCTTGGACCAGTTGGAG
AAGATTTTTTATATGAAGGGAAATTAGGAAAAGCAGCTTTCCTTGGAGCTTATCAAGCACAAAAAACTCC
GAAAGGAGAACTTACCTATGATTGGCTCCATCCTTTATCCATTGGAGCTAATGACGTTACTCCTGCTGAT
GCAAGCACCTGGTGTGAAACTCGACAGCATTGTGCCGCTGAGCTTCTTTTATCGGACTCTATATCCTCAG
ACGAACACCCTAAGGGTATTCGTCGAGAAAGAGTAAACCCGAATGATTTCTCTAAATTATGGAGTAAGGA
AGCTCAGGGAATCCTTTCTAGTGATTTCGCGGATCTTTTACCACGCTTTAACTTACTGATAGAATCATCT
GCGTTAGATATTCATACTTTATCGGCTCTTGATCGAGATATCCAACACCTTTTTACAAAAGTTCAAAAAG
ACCCTGTAGCATCTGTAGCTGTATTTTCTTTACAGTTACAATTAGCTGAAATGATTCGTGCGATTCCTTT
CCCTATACGTAATCAGGTACATATCTTGCCTGAAGCACAAGCACATTTCGAAGCAGATTGGAAGAAAGCA
ATCCAATTATATCTTCACAGTCATCCCCAAACAGAAGTGGTTATTTGGTACAGTTCTACTCATACTCAAA
TTGTTTTTGGAAAAGATTTGCTTGCTGTTGCAGAACGAGTTGCAGCAGCAAAATCCTTAATGTCTGATCA
TGATCCATCACTTATAACCAGTTATCTAAAATATAAAACTCAATCTCATCTTGGGGTGCTTACTGAGTTT
GATCAAGAAGATTTCTTTGAACTGATGGTAGATATTGCAGAGGAACCAGAGCTTCATAAACAACTATTGA
AAATAGAAGAACAAGTTAATTCAGGACTCTACTCTCATGTAGAGCATTCTTTAGGAGAGTGGTTAAAATT
ATCCAAAGAAGAAAGAAAATCAAAGTTTCTTAAGATTTTAAAAGAAACGTTTCAAGAGGAGGAAAGAGAA
GACTCTCAACAACAACATAAAACATGGTTTGAAGAACTCTATGAGAAAAGACATCAGGAGCGCGTAAAGG
ATCCAGCAAAAAAAATCCAAGAACTAATCACAGTTTTTCAAGAAAGTCAGCGCGTTCAAGCTCAAGACAT
AGATACTTATTTTGCTCATAAACCGTTTTATCAAGATCTGATGAAAGATGGTTACGCTTTTGAGGACATT
TCTGTGATTACGAAGTATCTTTTAGCGAGTGACGGTGTTTCAGGGATTATTACTACTGATCCTATATTTC
CTCCTCCATCTAAACAGCTAATTGACGCTATGAAACAATCTTTGGGAGAAGATTTTGGTGAGCTACACTA
TACGTTACAGATGGTCTATGATTGGTTATCTAAAGAGACTAACAGCGTAACTTCTGAACAGGCAAAACAA
AAATTACCACAGAAACTACATGAAAAACTAGAAGGTTATACAACACATGATTTGCTTATTCCGCCTATTG
ATGGAAGCGTCAGCGCTTTAGGATTGCGATTTTCCACGGAGGAGGGGAAGGTATCCGATCGCGTTTTAAC
AAGCATAGCACCTGGAGTTCCTAATTCTGCTAGCTATGCTATGACTAGTTATTTGTATGGTTTGTTCTTA
ATTACGAAAGATATCCAAAGTGGTCGATTAACCCATGAAATACTAAAAGAGAGATTACAAACCTATGGAG
GCGCCTATTTTATCAATGAATCAAAAATTGATGTGCTACTTGCTTTATCCCGGAAAAAAGCTCAGATATC
CCTAATAGACGCCCATAAGGCTCTTACAGGTTTTTCTTCTTTTAGCGAAGCATCTCTGGCTTTACTTACA
GGAAGGATGCCCGGAACAAGCAGAGTTCTATCAAGAGAAGTAGAGTTTGGACGACCTTCAGCTATCGTTA
TGGAAGGAGCAACAGCTATTCGTGCTCAGAGTTACGATGCTGTTGGATTAAGGAAAGATTTTTTCTTGTT
ACCTCATACAGTTCCTTCCATACAGTCTATTGTTGAGCAAGCGAAATATACAGTATTATCCTGGCCAGAA
TTTTATGAAAATCATGCGGATAAATGGAACGATCTTGCTAATCGTTTTGGAGCTGAGGACCTTAGTGTTC
ATCCGCAAACATTTTTATATGACACAGAAGGTCGCTGTATGGGGCTTGCTTTACTGTATATGTTAGCAGA
TGACTCTGTATCCTACAGATTATTACAACAAAATCTGATGACTTTAGCTTCGTTATTTGATGAGCAAAAC
AGAAGAAATATCCCTTTAACTCCAGCTGATCAGAAATTTTTAAATAAGGGGCTTTCTTTAATTGAGTGGT
TACAGTTCAAAGGAAATCAACAACTTCAAACAGAGGGATTTTTCCATACCTTAGACTGGGATATCCCTCA
ATTAATGAAGCACTTTGCTTCTTCAACTGTAAAAAGTTGGTTAATTACTACGCCTGCGCATAGTCTTGTT
CTTTCTTTAATGGGGAATTTTTTTAGAGTAACAGATCCAAACTATGGCCATACAGACTTTCCTTCATTAG
AAGCTGCTATTACTTTTTTAGAAAGGATGGTTCAAGTATCCCCAGCTGTTTTGGAGCGTTATGGATTTGA
TAAGGAAAAGTCTGTAACTAGCCAACTCAAAGTCCACTCTTTAGAGACTTCAGAATTGCAAAATGCAGTT
TTTGCTTCCTCAGATCTTGGCTTTACTAGTCGCTATTTTACGACAACGTTAGAAGAGATGACCGTCAGAG
GCCCTATTACGATGAATCAACGTCACACGGATTGGGCCACTCTTTATAAGATTGGGGGGACTGTTCAAGG
TAAACGTATTGATAGTAGAACACGGGAATCGGATTTAAATTTTTTAAAAATTAATGGGGATATTCTAGAG
GAATTTTTAACAAGAACTGTTCTAGATAGTGACTTGGTGGAGTTAATACAATCTCTGCTCAAAACACATG
GCCTTGAGCCAGGCACTACCCTTATTAGTCCAAGTTCTATTGTAGAGACCGCAATAGACCACGTCTCGCT
ATTACAAGCGGTGAAAACAAAAACCTCTCGAATGCACACTATTTTACAAAGCCTAGGAGAAAGAATTTTT
AAATTGTTTAAAAATTCTGGGGTACAAGACTCAGACAAAATTTCTATAGACAGAGTCCAACTAGTTGATG
AATCTGATAGCGCAACGATAGACTTCACAGTGATTAAAGATAAACAGCGTTCTCAGAAAAAAAGTATTAC
TGTTGGCATAGAAAGTTTAGCTGGATCCTTTAGAAAATTTAGCGCATCGATGCACGAAGTTATTGGAACT
GGAGTGCTTGATTTAGACTTAGGAATGACTGTCGTCTCTTTAGTTCAGTATGTCCGTTTAGTAGAAGCAG
GACAAGGAAAAGATGCTTTAGCAGTTGCTAATTTAGTGATGAATCTTAAAATAGCTCTCGAGGTCTCTAT
AGGGAATGTAATTCAGGCTCTGGGTAAAAAACTGCTCACTCAGGAGGGGCTTAATACTTTCCGTTTAGAA
ACAGAATTAGCTCGTCAACTACACAAAGTGGGAGCACGAGTTGGAGGAACTGTTGGTAAGACGTTAACCA
GAGTAGCGCATGTATTAGAACTCCCCATATTAGAAAGTGCAATTGGAACATGGAACCTCTATAATAGTGT
GAACGAGTTATTACATGCTGATTCTTGGAGTGACCAGGTTGCTGCTAGAGTACAAGTAGCTTTTGATTCA
ATCTCTTTAGGAATCACTATAGCTTCGGTTGTTTCTCCAGCACTTATGATGGCAGCAGGCCCCATTGCTG
CCATAGGGATGGGGGCAGCATCCATTGCTCGTAATGTTGCCAGAAAAGAAGAGCGGCACTCTCAATGGCT
CAAGTACAAGAGTTTTCTTGATAATGGATCAAAACATACAGTTGCCGCATTCCCTCATAAAGGGCTTATA
GATTTATCTGAGAATTTAGTACTTGGGAACATTGTGCTGAATTTAAGGGTTTACCCACCTCTGCTTACAG
GCGATCGCTCTTATAATGCAAATCGGTGGTTTGGGAATAAGCCTGGATGGTCTGATTGGCAAGTTCGAGA
AAGATTAGGTTATGCCTATCGAAGCAGTCCATCTTATGCGTTAGCCAGAGGACACGCTAATAGTTTTTGG
CCACTATCTATGCCTAGTATTGATAAAGGTGTATATCGCACAGTAATTCTAGGATATGGCATTCAATATA
AAGCGGTTACAGAAGTTGTCTACTTGTCTAATGAAATGGTTTGGAGAGAAGCTGTGATGCAGTTTGACTC
TCGATACTATGTAGAGCCATTAACAGCGGAAAAGGAGTGTGCAACAGTACTAGCAGGAGATACGCCACTA
TCAGTGATTCCTGTACGTTTATTAGAAGAGGAGTCTCTGGAACGCGAGAAGAACGCGGCTTCTTATAAGA
ACTATCAGATCATTATTGAGGGAGGGAAAGGAGGACTTACTGTACAGATAGGAGGAGCAGGTTATTACAA
GTTAACAGCACAGCCAGGAAAAGGGAATATCCTCTCTTTCCGAGCAATACCTGGATATCTTTCTGTAACG
TTTGATCTTAGCCGATTGGAACAAGAGGTGCCTCTCACCAAACAAAATGGAACAGCCTTAAAGATACTTA
AAGTACGTCAAACTGGGTTTGATACTATTGTTGGATCTTCAGGAGGTAGTGATAATTTAACAGGCAATCA
CAATACAAAATTTTATCTAAGCACCGGAGGAGGACATGTCACCTCAGGATCCGGTAAGAACTGGTATAAC
ATTCCATCTTTAACACGTAGCCTTGGTTTTGATTTTACTTCTAATGCTATAGAGCATAAGGCTTCTGTAG
AGATGCTTTTAGCAGATTTCTCTCCTGCTAATAATTTAAGTTTAATAGTTGAACGATTTGCAAATGTTAG
CATTCATGTTTTTAATTATAGCAATAAAACAGCCCCTTATACTGTGAATTTGAAAGACGGGGTGTCTTTA
CAAGCTTCTAAGCAAGGCTTTAGCGGTAGTTTATTAGAGGTGGCTTCCTTTGATCAAACGCTATGGCAAA
AGAGCTATCCAGAAGATAGAGGATTTGTAGAAGATATTTTATCTTGGTTATTAAAACTGCAATGGTCGTT
AGCTTCTCGAGTAAGGATCTTTTTACAGGGAGGCACAGCACAATACGAAACAACGCAGAAAAGTCTTATT
TATAGACCTGATCCACACTCAAGTATATCTATTCAGGCTACAGATTCTTATAATACACAAGTTTATGGTA
ACGTTGGCTGTTCTTATATTCTATTCTCTTCACCAGGTGTGACCGCCAAAACACTCGATATTTTGTTATT
CGAAGATTCGGGGTTACCACAAATTATTGACTTAAGCACACTTGTTCCAACTTCTATCCAAGGGTTCCTC
TATCCAGGAGGGTTTATAAACTTTCAAGTCTCTTCTGCAAGATATGCATTTCCTTTTTCTGTCAGTTGGA
GAAGGCATCATTACACTTTCCCTGCTCAAACGATTATTCAAGTTCTACCTCGCTTGCGGTTTGAACTAGG
GGATTGGTTTACAATTTTGCAAAAGAGTGTAGGAAAATGGGTAACATTGTACCAACACGACATGATTATC
CCTGAACGTATTGAAGGTGTTTTAAGTCTAAATAACACAGCGACATTAATGTCACACCACAATCAAACAA
CCCATATGCTTGGAGTAGAAAACAGGGGAGATTTGAGTCTAAAAGTTTTAGGAATACTCCAATCTGGTCA
AATTAAAGGATCTAAATCGGAAAATAGCGCGAGTGGTCATTTTTCTAAATTGTTATCTGTCCCAGCACAT
ACGATCAAAAATCTTTCGTTTAAAGAGGAAGAGGGAACTAAGAGTAAAAATATTTTATTCTACAGTGTGC
TGGAGGAAACATTCCTTAAAGCTACAGATAAACCTTTAACTATTATTAAACGTGATAAATGGTCTCTGTA
TGATGAAATCCAAGTATTTGCAACAACTCTTAATCTGCAAAATTTCTTGCGTTATCATATTTCGGAAGAG
ACTCCAATGTTATCTAGACTGCTCATGTATGCGCAGAAACGCGTTTCCATCCAAAATAGAGATCTCGCAT
TGAAATTTTTCTATGTTCGAGAGCGAAGTGGTATTGGTGCAATTAGATTGGTATTCAAGAACTTTTTCGA
AGAGAGCTTGCAGGGGATATTACATGGAACCTTAGAGCGAGAAGTTAAACCTATGCTAGCTGAAAATCCA
AATACACTAATTCATTCATCGTATCGGAATCATTTAGAACTCATCCTAGGGGAGGAAACCTTAGATCTCG
CAATTATCGTTCAAGAGTTTAGCTCTTCAAAAAATATTGTTGAGTTGCAAAAAGATCTAGCAACCCATCG
GCTTATTTATCCTCAAGGAAAAGAAGCCTTGTCTCTTGCAATCTATACCCATACTTTCAGTAAAGAGAGC
TTAGTCACATCTAAACAACGAAGCGAAGGGTTAAAATTCATCGATCCATCAATGCAGGAATATCGTTTAC
CTGAGACAACGATTCTCGAAAACTCTTATTATTTAGACCCTTCCACAGGAGATTTGTATTTAACAAAGAT
AATTGCGCAACCTTCACAAAGTAGAGCATTTCTTATCAAATTTAAAAAATACAAACGCCACTGGCTAGAT
TTCCAAAAATTAGTTCTTTCTGGATCTCATTTAGAACTTATCGCATCTACTGGAACAGCTTTAACATTTG
TAGGCCCCGAATTACGGCATTTAGAGATTGACTTCCCAGGCGCGTTAAATGGGACTATTGCCAAAGAACA
CATCTCATCTCGGGCAAGTATTGTAATCCCAACAAACAATCAGGTGACACACTATGACCCAAGAATAGCG
AAGCAGCATTATAGCTCTATCAATTATATGCTGTGGGATCTGCGTGATCGTGCGACACTATCTAAGCGAG
CAAAAACTTTAGATAGCTATTTGTTAGAAGTTTGTATGAACTTAGACTCTTCTAATCCAACGTGGAATAT
TCCAAATGATGTGTTGAACTACGCGGTAGGATATTATCGAACAATCCTTCCTAGCTGGGTAAAAAATAAA
ATCCGTGTAGGTTCCTTAATTAAAGTAGAAGCTGATACTGCAAAAACTTTGAGTTTCTCACTAATTACGA
CACAAAATGATCTATTCCAACCTGTAGTGGATAAAGGATTCTATATTTACTACAGCGTTTCGAAACTAGC
CAATCATGTTAAACTGCGAAATGTTACTGGAGATATGTTGCTTGATATCGATAAGGAAACCACATTTATT
GTTCGAGGAGTAGATGAATCTGATTATGATAAGAAACAAATCTATGTTGTTTTAGATCTGACAACAGAAG
AAGAACGTAAATTGCGAACGGATAAGAACATCATTATTATTCCTGGAGGAGAACGATTGAACCGATG
-
Protein Sequence : Show Sequence
>WP_010230453.1 LifA/Efa1-related large cytotoxin [Chlamydia muridarum]
MTLSNTASSTVSPKSTQLNHTLSNQTHPVAQSNRNIDFQIYDEQIHNNHSTEDVVAIGRRIQQEYANLTT
SKQVNFSASPSNHTGNWKISLLYNLSMLVANLFPTTIQPVQPQKVIFKNQDSSKETQLTKSSKVVSSRSS
LKEIFSKKSPPLRKSFSSSKISRAIPRRKKRSNDDPVQATNTYDLTAENILEKLSLTQEQQIKHDNLISN
LKEAINRYSDLNRKNSRKGQSLLVRQAKILDEILSQTKSTEERASNSVMTTIKKEFTSHRVPVEKNIHGI
WIAGSPPEGTDEYIKLFLHTYPEFSFLFWVDKTAYGAAKFSSTLKRIAFDAAVNSLREATPEPVKQFVQR
YDKLKKSYDTSRDFDEKQRLSEQLVELYDNYNKFSKEIQSNFDVLLLHEMITIQDSFFNYCQLKGVGAIT
DETRIEYLEKVLKVKEEDLSHYKETIKRNKESIEKLVKEINDSTGRERVVIKDIRDLKSLQDLTNSYNYE
TEMLLRWNYAAATDQLRMYMLKEYGGIYTDLDIMPQYSQDVLQKIMDVGGSRFFEHDKLRRTLSFAALKL
GSGKQTTVSFEEAKKAMTLPTFTLQDKSQISEIFKYLETETQAKKSLFQPMDVTVVRDFMPILQRYHKWQ
TGWNVRGLNGLMMAHKDSAVVDAVIARQRAAYDEMRALRQNVVSGEFFRSLGDLEHVNREKNIGGYLAKN
YLGGSLFFDFRQDSVIPGAISTLGISGPDIIMDTMSDYFTNLGPVGEDFLYEGKLGKAAFLGAYQAQKTP
KGELTYDWLHPLSIGANDVTPADASTWCETRQHCAAELLLSDSISSDEHPKGIRRERVNPNDFSKLWSKE
AQGILSSDFADLLPRFNLLIESSALDIHTLSALDRDIQHLFTKVQKDPVASVAVFSLQLQLAEMIRAIPF
PIRNQVHILPEAQAHFEADWKKAIQLYLHSHPQTEVVIWYSSTHTQIVFGKDLLAVAERVAAAKSLMSDH
DPSLITSYLKYKTQSHLGVLTEFDQEDFFELMVDIAEEPELHKQLLKIEEQVNSGLYSHVEHSLGEWLKL
SKEERKSKFLKILKETFQEEEREDSQQQHKTWFEELYEKRHQERVKDPAKKIQELITVFQESQRVQAQDI
DTYFAHKPFYQDLMKDGYAFEDISVITKYLLASDGVSGIITTDPIFPPPSKQLIDAMKQSLGEDFGELHY
TLQMVYDWLSKETNSVTSEQAKQKLPQKLHEKLEGYTTHDLLIPPIDGSVSALGLRFSTEEGKVSDRVLT
SIAPGVPNSASYAMTSYLYGLFLITKDIQSGRLTHEILKERLQTYGGAYFINESKIDVLLALSRKKAQIS
LIDAHKALTGFSSFSEASLALLTGRMPGTSRVLSREVEFGRPSAIVMEGATAIRAQSYDAVGLRKDFFLL
PHTVPSIQSIVEQAKYTVLSWPEFYENHADKWNDLANRFGAEDLSVHPQTFLYDTEGRCMGLALLYMLAD
DSVSYRLLQQNLMTLASLFDEQNRRNIPLTPADQKFLNKGLSLIEWLQFKGNQQLQTEGFFHTLDWDIPQ
LMKHFASSTVKSWLITTPAHSLVLSLMGNFFRVTDPNYGHTDFPSLEAAITFLERMVQVSPAVLERYGFD
KEKSVTSQLKVHSLETSELQNAVFASSDLGFTSRYFTTTLEEMTVRGPITMNQRHTDWATLYKIGGTVQG
KRIDSRTRESDLNFLKINGDILEEFLTRTVLDSDLVELIQSLLKTHGLEPGTTLISPSSIVETAIDHVSL
LQAVKTKTSRMHTILQSLGERIFKLFKNSGVQDSDKISIDRVQLVDESDSATIDFTVIKDKQRSQKKSIT
VGIESLAGSFRKFSASMHEVIGTGVLDLDLGMTVVSLVQYVRLVEAGQGKDALAVANLVMNLKIALEVSI
GNVIQALGKKLLTQEGLNTFRLETELARQLHKVGARVGGTVGKTLTRVAHVLELPILESAIGTWNLYNSV
NELLHADSWSDQVAARVQVAFDSISLGITIASVVSPALMMAAGPIAAIGMGAASIARNVARKEERHSQWL
KYKSFLDNGSKHTVAAFPHKGLIDLSENLVLGNIVLNLRVYPPLLTGDRSYNANRWFGNKPGWSDWQVRE
RLGYAYRSSPSYALARGHANSFWPLSMPSIDKGVYRTVILGYGIQYKAVTEVVYLSNEMVWREAVMQFDS
RYYVEPLTAEKECATVLAGDTPLSVIPVRLLEEESLEREKNAASYKNYQIIIEGGKGGLTVQIGGAGYYK
LTAQPGKGNILSFRAIPGYLSVTFDLSRLEQEVPLTKQNGTALKILKVRQTGFDTIVGSSGGSDNLTGNH
NTKFYLSTGGGHVTSGSGKNWYNIPSLTRSLGFDFTSNAIEHKASVEMLLADFSPANNLSLIVERFANVS
IHVFNYSNKTAPYTVNLKDGVSLQASKQGFSGSLLEVASFDQTLWQKSYPEDRGFVEDILSWLLKLQWSL
ASRVRIFLQGGTAQYETTQKSLIYRPDPHSSISIQATDSYNTQVYGNVGCSYILFSSPGVTAKTLDILLF
EDSGLPQIIDLSTLVPTSIQGFLYPGGFINFQVSSARYAFPFSVSWRRHHYTFPAQTIIQVLPRLRFELG
DWFTILQKSVGKWVTLYQHDMIIPERIEGVLSLNNTATLMSHHNQTTHMLGVENRGDLSLKVLGILQSGQ
IKGSKSENSASGHFSKLLSVPAHTIKNLSFKEEEGTKSKNILFYSVLEETFLKATDKPLTIIKRDKWSLY
DEIQVFATTLNLQNFLRYHISEETPMLSRLLMYAQKRVSIQNRDLALKFFYVRERSGIGAIRLVFKNFFE
ESLQGILHGTLEREVKPMLAENPNTLIHSSYRNHLELILGEETLDLAIIVQEFSSSKNIVELQKDLATHR
LIYPQGKEALSLAIYTHTFSKESLVTSKQRSEGLKFIDPSMQEYRLPETTILENSYYLDPSTGDLYLTKI
IAQPSQSRAFLIKFKKYKRHWLDFQKLVLSGSHLELIASTGTALTFVGPELRHLEIDFPGALNGTIAKEH
ISSRASIVIPTNNQVTHYDPRIAKQHYSSINYMLWDLRDRATLSKRAKTLDSYLLEVCMNLDSSNPTWNI
PNDVLNYAVGYYRTILPSWVKNKIRVGSLIKVEADTAKTLSFSLITTQNDLFQPVVDKGFYIYYSVSKLA
NHVKLRNVTGDMLLDIDKETTFIVRGVDESDYDKKQIYVVLDLTTEEERKLRTDKNIIIIPGGERLNR
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0439 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
- Related Vaccine(s):
C. muridarum DNA vaccine encoding TC0439
|
11. TC0512 outer membrane protein, putative |
-
Gene Name :
TC0512 outer membrane protein, putative
-
Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
-
VO ID :
VO_0010885
-
NCBI Gene ID :
1245872
-
NCBI Protein GI :
497916494
-
Locus Tag :
TC_RS02600
-
Genbank Accession :
AE002160
-
Protein Accession :
WP_010230650
-
Taxonomy ID :
243161
-
Gene Starting Position :
618235
-
Gene Ending Position :
620613
-
Gene Strand (Orientation) :
+
-
Protein Name :
outer membrane protein assembly factor BamA
-
Protein pI :
9.51
-
Protein Weight :
84655.32
-
Protein Length :
792
-
Protein Note :
similar to GB:X75201, and PID:435005; identified by sequence similarity; putative
-
DNA Sequence : Show Sequence
>NC_002620.2:618235-620613 Chlamydia muridarum Nigg, complete genome
AATGCTTGGAATACGAAAAAAAACGATTCTGCAACTCGCTATTTTACTGTTGCTCCCCTTTTCACAAAAC
GCCTTTGGAACATCTCCTGAAGGACGTATGGTGGTACAATCCATCACTATTGAGACTCGAGGAGAAAATG
CTCAAAATAAGCGGGCTATTCCCAAAATAAAGACAAAGCAAGGGACGCTGTTCTCTCAAACTGATTTTGA
CGAAGATCTAAGAACTCTCTCGAAAGATTTCGACCGCATTGATCCTATTGTAGAGTTTCGCAATGGGCAA
GCTGTTATTTCCCTTATTCTGACAGCAAAACCCGTTATCCGACACATTAACATCTCAGGGAATGAAGCAA
TTCCTACTCATAAAATTTTAAAAACCCTAGAGCTTTATAAAAACGATCTTTTTGATCGAGAGGTTTTTTT
CAAAAATTTTGATGCTTTAAGAACCCTTTACTTAAAACGCGGATACTACGATTCTCAATTGTCCTATTCT
CACGATCATAATGAGCAAGAAGGTTATATTGATATCTCAATCCAAATTAAAGAAGGGCCCTACGGACGGA
TAAAAAAATTAACAATTTCAGGTATTACTCGTACAGAAGCCTCTGATTTGGGAGACATAGTTTTAACCAA
ACAGTACTCTACAACAACAAGTTGGTTCACTGGAGCAGGGGTATACCATCCAGACATGGTGGACCAGGAT
CTCTTTGCCATAACAAACTATTTTCAAAATAAAGGTTATGCAGACGCAAAAGTCACAAAAGAAGTCTCCA
CAGATGCTAAAGGAAACCTCTCTCTTGTTATCAACGTAGATAAAGGGCCCCTGTATACTTTAGGTCACGT
ACATATAGAAGGTTTCACAGCTTTATCCAAGCGGTTACTTGATAAACAACTGTTAGTGGGCCCCAATTCC
TTATATTGCCCAGAAAAAGTTTGGGCTGGAGCTCAAAAAATCCGTAATGCCTACGCTAGATACGGATATG
TAAATACCAATGTAGACGTATCTTTTGCTGTTCATCCAACGCTCCCAGTTTATGATGTTACTTACAGGGT
TAGCGAAGGAGCTCCTTACAAAATCGGACTAATCAAAATCAAAGGGAACACACACACCAAACATGACGTA
ATTTTACATGAGACAAGTCTGTTCCCTGGAGATACTTTTGATAGATTAAAACTAGAAGATACGGAAACTC
GATTACGTAACACAGGCTACTTTAAAAGTGTGAGTGTCTACACGGTACGCTCACAATTAGATCCTTTGGA
CTCAAATAACCTTTATAGAGACGTTTTCATTGAAGTCAAAGAAACCGAAACAGGTAACCTAGGACTGTTC
TTAGGATTCAGTTCTATAGACCATCTGTTCGGAGGAGCAGAAATTTCAGAAAGCAATTTTGATTTATTTG
GCGCCCGACACTTATTCAAAAAAGGGTTTAAATCCTTAAGAGGTGGTGGGGAATATCTTTTCTTAAAAGC
CAATTTAGGAGACAAAGTTACTGATTATACTGTTAAATGGACAAAACCTCATTTCTTAAACACCCCATGG
ATCCTAGGAGTAGAATTAGATAAATCTATTAATAAGGCTTTATCAAAAGATTATTCCGTTGATACCTATG
GAGGAAACATCAGCACGACATACATTCTTAACGACAAACTGAAATATGGGATGTATTACCGCGGGAGCCA
AACGAGCCTAAGTTTAAGAAAAAAAACAGAAAATCCTAACAAACCTGGACCAGATCTAGATAGTAACAAG
GGATTTGTTTCCGCGGCAGGGCTTAACGTTCTTTACGATTCTATTGACAATCCTAGAAAACCTACTATGG
GTATTCGGAGCTCTTTGAACTTTGAGTTGTCTGGATTAGGAGGAACCTATCAGTTTACAAAATTAACAGC
CAGCGGCTCTATCTATCGCCTGCTAACTAAAAAAGGTGTTTTGAAAATTCGTGGAGAAGCTAAGTTTATT
AAACCTTTCGGTACAACCACAGCCCAAGGAATTCCTGTTAGTGAGCGCTTCTTCTTAGGAGGAGAATCTA
CCGTTCGTGGATATAAACCATTTATTATTGGGCCTAAATTTTCTCCAACAGAGCCTCAAGGGGGCTTATC
TTCCTTATTGCTAACAGAAGAGTTTCAATATCCTCTAATTTCTCAACCCAGCATCAATGCATTCGTTTTT
CTAGATTCGGGCTTTATTGGCACGGAAGAGTACACTATTCGACTCAAAAATCTCTGTAGCAGTGCCGGAT
TTGGGTTGCGTTTTGATATGATGAATAACGTGCCCATTATGTTAGGTTGGGGTTGGCCTTTCCGCCCAAC
AGAAATTCTTAATAATGAAAAAATTGATGTATCTCAAAGATTCTTCTTCGCTTTGGGAGGAGTGTTCTA
-
Protein Sequence : Show Sequence
>WP_010230650.1 outer membrane protein assembly factor BamA [Chlamydia muridarum]
MLGIRKKTILQLAILLLLPFSQNAFGTSPEGRMVVQSITIETRGENAQNKRAIPKIKTKQGTLFSQTDFD
EDLRTLSKDFDRIDPIVEFRNGQAVISLILTAKPVIRHINISGNEAIPTHKILKTLELYKNDLFDREVFF
KNFDALRTLYLKRGYYDSQLSYSHDHNEQEGYIDISIQIKEGPYGRIKKLTISGITRTEASDLGDIVLTK
QYSTTTSWFTGAGVYHPDMVDQDLFAITNYFQNKGYADAKVTKEVSTDAKGNLSLVINVDKGPLYTLGHV
HIEGFTALSKRLLDKQLLVGPNSLYCPEKVWAGAQKIRNAYARYGYVNTNVDVSFAVHPTLPVYDVTYRV
SEGAPYKIGLIKIKGNTHTKHDVILHETSLFPGDTFDRLKLEDTETRLRNTGYFKSVSVYTVRSQLDPLD
SNNLYRDVFIEVKETETGNLGLFLGFSSIDHLFGGAEISESNFDLFGARHLFKKGFKSLRGGGEYLFLKA
NLGDKVTDYTVKWTKPHFLNTPWILGVELDKSINKALSKDYSVDTYGGNISTTYILNDKLKYGMYYRGSQ
TSLSLRKKTENPNKPGPDLDSNKGFVSAAGLNVLYDSIDNPRKPTMGIRSSLNFELSGLGGTYQFTKLTA
SGSIYRLLTKKGVLKIRGEAKFIKPFGTTTAQGIPVSERFFLGGESTVRGYKPFIIGPKFSPTEPQGGLS
SLLLTEEFQYPLISQPSINAFVFLDSGFIGTEEYTIRLKNLCSSAGFGLRFDMMNNVPIMLGWGWPFRPT
EILNNEKIDVSQRFFFALGGVF
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0512 showed significant levels of protection when compared to negative control animals. At 9 days following challenge TC0512 showed a 73% reduction in the number of recoverable Chlamydia compared with vector only immunized controls (McNeilly et al., 2007).
- Related Vaccine(s):
C. muridarum DNA vaccine encoding TC0512
|
12. TC0757 hypothetical protein |
-
Gene Name :
TC0757 hypothetical protein
-
Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
-
VO ID :
VO_0010886
-
NCBI Gene ID :
1246122
-
NCBI Protein GI :
497917305
-
Locus Tag :
TC_RS03850
-
Genbank Accession :
AE002160
-
Protein Accession :
WP_010231461
-
Taxonomy ID :
243161
-
Gene Starting Position :
898853
-
Gene Ending Position :
899647
-
Gene Strand (Orientation) :
+
-
Protein Name :
hypothetical protein
-
Protein pI :
5.02
-
Protein Weight :
27979.63
-
Protein Length :
264
-
Protein Note :
identified by match to TIGR protein family HMM TIGR01607
-
DNA Sequence : Show Sequence
>NC_002620.2:898853-899647 Chlamydia muridarum Nigg, complete genome
CATGCGCTGTTCTGCTTATTGCTCTGCTTCGTCCTATCATCTACACGTTCTTTTTCATCTTTTAAAGGCA
CGCTACCCTTCTGTCTTATCCCGAGAATACGTGCTCATTTCCTCTGAAGAGCTGAATGAAAGCGACAAAG
CCGCTGTCTTTTTCCCATTTGGAGTTAGTGTGTTTTGGGGATGGGAAGAAGCTGAAGAACTTCAAGTGAT
CCAAGCTATCACCTCAGCATCAGTGGATCCTCTCCCTAACCCAGAAATTGATAGTTATGACTTCCATTAT
GGAGAGAAACTACAGATCCGTCGCGATAGACTTGTTCTCACCAACTCTAATTTAAACACAAAACTTGCTA
TTTCCTTTGGGTTAGCCCAATCTATCAAACTTACCGTCTTTGAAGAAACGATTTACAAAACAGTTGAAAA
CTCTAAATCTTTGCCTCAAGAACTTGCCTCTAAAGGGAAAATTTCTCTATCCCGTAAAACGATCGCAAAA
AAGATTGGAGAGCTTTTCCTGGATAAAGCTTCCGTAAACCTACACTCTGACATTCTCGATGAGCCCGACT
TTTTCTGGGAACATCCAGAAACCCAACCTTTTTATATTGATGTGTTGACCTGCCTAGATGTAAACGCGCG
AGTAAATGTATTAAATCACAGACTTGCTATCCTAGGAGATGTTCTCGAGATTTTAAATGACCAACTTAAT
CATCAGCATTCTTCAGCTCTAGAGTGGACCGTGATCTGGTTAATTGCACTAGAGGTATTGGTCACCTTAC
TTAAAGACGTATTCAACATCATCTA
-
Protein Sequence : Show Sequence
>WP_010231461.1 RMD1 family protein [Chlamydia muridarum]
MRCSAYCSASSYHLHVLFHLLKARYPSVLSREYVLISSEELNESDKAAVFFPFGVSVFWGWEEAEELQVI
QAITSASVDPLPNPEIDSYDFHYGEKLQIRRDRLVLTNSNLNTKLAISFGLAQSIKLTVFEETIYKTVEN
SKSLPQELASKGKISLSRKTIAKKIGELFLDKASVNLHSDILDEPDFFWEHPETQPFYIDVLTCLDVNAR
VNVLNHRLAILGDVLEILNDQLNHQHSSALEWTVIWLIALEVLVTLLKDVFNII
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0757 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
- Related Vaccine(s):
C. muridarum DNA vaccine encoding TC0757
|
13. TC0767 hypothetical protein |
-
Gene Name :
TC0767 hypothetical protein
-
Sequence Strain (Species/Organism) :
Chlamydia muridarum str. Nigg
-
VO ID :
VO_0010888
-
NCBI Gene ID :
1246132
-
NCBI Protein GI :
497917334
-
Locus Tag :
TC_RS03895
-
Genbank Accession :
AE002160
-
Protein Accession :
WP_010231490
-
Taxonomy ID :
243161
-
Gene Starting Position :
910619
-
Gene Ending Position :
911350
-
Gene Strand (Orientation) :
-
-
Protein Name :
polysaccharide deacetylase
-
Protein pI :
9.14
-
Protein Weight :
26828.18
-
Protein Length :
243
-
DNA Sequence : Show Sequence
>NC_002620.2:910619-911350 Chlamydia muridarum Nigg, complete genome
ATTAATGCAAAATAAGGAATCGTTTTAATTGGCGGAAAGAGAGCTGATAAAGAGATGGGATCCCTCGACT
ATCTAAAGCCATATCAATACGAGGAATGCTATGCTGTGATTGCATCGAGTAAAAAAAACTCGCAGCATCC
CCTAACACAAAAGAATAGCGGTAAACCTCCTGAACAAAATGTTGACTCACCAAATCACTTTTTCCAAAAG
GATAAAAAAAGACTTCCACAGGAGCCTGTATAGCCTCTTCTAGTAAAATTTTAGACAATGAAATTTCAGT
ATGTAAATAAGGAGGGGAGTTTTTAAGGTTTCTAATAGCAAACCCTGAAGAGGCAAATCGAATAATCGAA
CTTTGAGCCATATGACAAAGTTCTTTAACCGAACAAAAGGGCTGATAATAAGAAAATATTTCGTCTTGAA
AAGCTAGAAAATCCGATGGCGAAATTCGCATATCAATGGGAAGATTTTCACTTTCTAAACGGGATACGTA
TCTCCAAGCAACTCCAACAACCGCAGGAATCTGGAATTTCTGAAGAAAAGGAAAGACATGCTTGTAAAAA
TCAACAGAAGCATAATCGAATGTCAGTATGATTGCTTTCCTATTAGGGAGAGGATCTCCGGGCAGAATAA
ACGAATAATGCTGCTTGAGAGAGTGCAAAAAATCTAAAAACGATCTGAGGGCATAAGGAAACTTAGAAAA
AGCAACCTGTCGATAGGCTAAGACGCGGAGCA
-
Protein Sequence : Show Sequence
>WP_010231490.1 polysaccharide deacetylase family protein [Chlamydia muridarum]
MLRVLAYRQVAFSKFPYALRSFLDFLHSLKQHYSFILPGDPLPNRKAIILTFDYASVDFYKHVFPFLQKF
QIPAVVGVAWRYVSRLESENLPIDMRISPSDFLAFQDEIFSYYQPFCSVKELCHMAQSSIIRFASSGFAI
RNLKNSPPYLHTEISLSKILLEEAIQAPVEVFFYPFGKSDLVSQHFVQEVYRYSFVLGDAASFFYSMQSQ
HSIPRIDMALDSRGIPSLYQLSFRQLKRFLILH
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
- Related Vaccine(s):
C. muridarum DNA vaccine encoding TC0767/768
|
14. TC0768 hypothetical protein |
-
Gene Name :
TC0768 hypothetical protein
-
Sequence Strain (Species/Organism) :
Chlamydia muridarum Nigg
-
VO ID :
VO_0010889
-
NCBI Gene ID :
1246133
-
NCBI Protein GI :
15835382
-
Locus Tag :
TC_0768
-
Genbank Accession :
AE002160
-
Protein Accession :
NP_297141
-
Taxonomy ID :
243161
-
Gene Starting Position :
911335
-
Gene Ending Position :
911496
-
Gene Strand (Orientation) :
-
-
Protein Name :
hypothetical protein
-
Protein pI :
3.69
-
Protein Weight :
5224.4
-
Protein Length :
53
-
DNA Sequence : Show Sequence
>NC_002620.2:911335-911496 Chlamydia muridarum Nigg, complete genome
GCTAAGACGCGGAGCATGGATCAACTGAAGGAATTGCAAAAGGGTCCTCTGGATAAATGACTACCTGTCC
GGAAATGATCGTTAGATTACGCTCTTTTCCCTCAGGAGTAAGCTCTGCTGTTTCCGTAGAACAGACTTCA
GAAGTAGGTAAGGAGATCGGCA
-
Protein Sequence : Show Sequence
>NP_297141.1 hypothetical protein TC_0768 [Chlamydia muridarum str. Nigg]
MPISLPTSEVCSTETAELTPEGKERNLTIISGQVVIYPEDPFAIPSVDPCSAS
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
- Related Vaccine(s):
C. muridarum DNA vaccine encoding TC0767/768
|
15. Virulence plasmid protein pGP3-D |
-
Gene Name :
Virulence plasmid protein pGP3-D
-
Sequence Strain (Species/Organism) :
Chlamydia muridarum
-
NCBI Protein GI :
WP_010231988
-
Other Database IDs :
CDD:283197
-
Taxonomy ID :
83560
-
Protein Name :
Virulence plasmid protein pGP3-D
-
Protein pI :
4.33
-
Protein Weight :
26436.35
-
Protein Length :
340
-
Protein Note :
Chlamydia virulence protein PGP3-D; pfam05475
-
Protein Sequence : Show Sequence
>WP_010231988.1 Virulence plasmid protein pGP3-D [Chlamydia muridarum]
MGNSGFYLHNTSNCVFADNIKVGQMTEPLTDQQIILGTSTTPVAAKITASEGISLTITNNAQANSSVNIG
LDAEKAYQLILDKLGDQIFDGITGSIVESAVQDIIDKITSDPSLGLLKAFYNFQITGKIQCNGLFTSSNV
TTLLGGTEIGRFTVTPRSSGSMFLVSADIIASRMEGGVVLALVKEGDTQPCAISYGYSSGVPNLCSLKTC
VTNSGSTPTTYSLRIGGLESGVVWVNALSNGNDILGITNTSNVSFLEVIPQKNT
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
|
III. Vaccine Related Host Genes |
1. Ifng (Interferon gamma) |
-
Gene Name :
Ifng (Interferon gamma)
-
Sequence Strain (Species/Organism) :
Mouse
-
NCBI Gene ID :
15978
-
NCBI Protein GI :
33468859
-
Genbank Accession :
NM_008337
-
Protein Accession :
NP_032363.1
-
Other Database IDs :
MGI:107656; UniProt: P01580
-
Taxonomy ID :
10090
-
Gene Strand (Orientation) :
?
-
DNA Sequence : Show Sequence
>gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
AGTATACCTTTGCAACC
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Protein Sequence : Show Sequence
>gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
LLPESSLRKRKRSRC
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Molecule Role Annotation :
IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
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Additional Molecule Role :
Vaximmutor
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Additional Molecule Role Annotation :
The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
- Related Vaccine(s):
C. muridarum MOMP protein vaccine
|
2. IgA |
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Gene Name :
IgA
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Sequence Strain (Species/Organism) :
Mus musculus
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NCBI Gene ID :
238447
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Genbank Accession :
AC160982
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Taxonomy ID :
10090
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Chromosome No :
12
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Gene Starting Position :
8607
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Gene Ending Position :
12639
-
Gene Strand (Orientation) :
-
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Protein Name :
immunoglobulin heavy constant alpha
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Protein Note :
Also known as IgA; Igh-2
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DNA Sequence : Show Sequence
>gi|121699722:8607-12639 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
AGTACTGGGGGACCTCTTTGCTGCCAAACGGGCCTCGAACAGTTGTAACAGTGAGTGCTGTGCTGTAGAA
CAAGCTCAGTAGGAAGAGGGTGAGGAAGGTCACAGTGGTGGGCCACAGGCTGGCACCTGGGGCCTCTTCC
TCCAGGATGTCTTCTGACTGGTCCAGTAGCACATAGGAAAGTGGCTCTTGACGTTCTGTAAGACATAGAT
GACCTTCTAGATCCAAACCCATGGGGATGGGGGCTCAGACTTAAGGTTTCTGCTTCTATGCCTCCTTGGG
CTTTGGGGACAGTGGTGGTAGGGGTCCCTTATTCCCGGGAAGGGACTTGTTCAGGGATAATGAGGAGGCA
GGGTCCCAGGTAAGGGACTGGAATCCCAGCAGTTCTTGAGATGGCCCCAAGACCCATCTCTCTCTCTTAG
GGAAACCTTAATTAGAGACCTGTCATGGTAAGAGAGTTGTTTCTGTCAAACTCTGAGAAGAGTTTGAACC
CAAGGCTCCTTAGGTCTCAGTCCTTTCTAGATATTTAGCAATACCTCCCTAAACCCCAGTTCTTAGGCTA
ATCTTGGCTTGGGTACTAGGGTTGTGTAAGATCAGACACCTTCAAACAGATACAGGCCAATGTGATTTCT
GGGCCAAAGTTAGACTACCAAGGCCTGGACATGTGGTCAGAGGACTCTGTTGCATTCTTAAGAGCTGTGA
CTGGGGGTTAAGCAACAGTAAAGACCCCCAGTAGAGCCAACCCTGCTGTCCTCAGGTTCTCTGCGCACAG
AGTTTCATTTTAAGTGGCAGTAGGAAATGTGTACTGATAGTCAACCACTCGTATTTCTGTCACTGCAGCC
ATCCATCAATCCATCCATCATCCCCATCGTTCATCCTTGTAGCCAGCCATCCATTACCCTCCAGCCGCTC
ACTCATCATCTTTCCCGACTATCGTGATTCTCCAGCTTCTCTGCCCCCTCTCCCTGTGGGGATTCAGTCT
TTCACCTATTGACTCCTCCTTACTAGGATTCTCTGTCATGGGCCAGGCCTTGGATTCCATGGGTGGCTAT
GGTACTTTTCTGAGTCAGGTTTCCCAAGCTGGTCTGTTGCTCTTGCTATTCTCAGGGAACTGGACTTTCA
AGCTGGAGAGTATCCAGGGCATGCATGGGCCTTTACTCCACTCTTGCTCTGAGAGCAGAGGGAAGTTGCT
AGAAACCAGCAACTCCTCCCCCCCCCCCCCGAAGTTCTGAAGGCCTGTGAGACAGGAGCAGGGCCCCCAC
TGCCCTCTTGTGGACACCTGGGCTCCAAGTATACCTGGTTGTAGAACTTTGTGTTCTAGGGGTGCCCATG
GAGGAGGCCGAGGCCTGTGTGGGAGTGGTCTCCATGGCCTGCTCCTGCTTCTTCCCAGATTTGCCTCTTA
TCCTGCTTCTGGTTTGTGATACTTGGGCTCAGGATGGGAAGGGAGACTTAGGTTAGAAAGGGGGCACCTG
GAGGGGCCTATGCCCTTGTCCATTGTGTGTAGTTGTCAGTGTCGGGCCTGGAAATTCTCCCGGGGGCTTG
GAGGGGACTCCCCTTGCTTTCCAGAAGCTGGGTGTGGGCTGTGAACTTCATCTTCCTCCAGGGTTCAGGT
CTGTGTGAGTGTGCAAATGTGAGTGTCTGTGCATGTATGCCCATGTGCATGTATGCAGAACAGGGTGTAT
GCCCATGTGCATGTGTGAAGAGCAGGGTGCTGCTGAGGGTTGTCTGTGCCATGCTATATGTCTTTCTCAT
GAGTCAGTGGTCACCCTGAATGCATGTTTCCATTCAGGGATGGCCTACTGAGGGTGCGTGGCATCTTCTT
CCCAGTGCCCCTGTGTTCATCCCTTCATTCTCATACCATCATCTCTATTGTGACCCCCACACGGCACCAG
CCTTGATGGTGCTACCCTTTTGCTCCATCTATCCCTGTCTTGTTTGGGTTAGAAGCTGCGGTCTAATAAA
CAGCCACACCACCCACCACTCAGAGTACTTGTGCAGCAACCCACTGCCCTGGGGACTCTCCCATGAGCTT
TGCCTTCTTGGCTGAGTCTGCTCGCCTTGGATTTCATCCCCAGTTTATTCTTACTTCCCATTCACTCATT
GCACATTCACAGGGCAGGTGCACACACTCTCATGTTTCCACCAACATGAACACACATAGATATATGCATG
CTCATAGGTATGCACAGGCACACACTTGCTCACAGGGACATAGATATAGTGTGTCCACATATGTACTTAT
ACCCATATACATACCTATAAACCATAGGTGCACACAAACTCACTCCTCTCTTGCTGCACACAGGTACACA
CATGAAGGCACATGGACCCATCCATAAGCACTCACCAATACATAGACATGCATACACATATACTCTAGGC
ATGAAGGGTCTTCAAAGTCTCCGAGGCCTTACAAGCTTAGGTGGTGGTGTTTCTCCCTCCCTGGGCCCTG
ACCCCTCCCTGTGTTCTGTAGACCCTAAGGATGATGGACAGGCACTGGATGGAAGTGCAGGGATACTTTG
GATGAGCACAGAGTTTATTTCAGGAGTAGGGACAGGCAGGGTGGCTCAGTAGCAGATGCCATCTCCCTCT
GACATGATCACAGACACGCTGACATTGGTGGGTTTACCCGACAGACGGTCGATGGTCTTCTGGGTGAAGT
TCATGGGCAAGGCCTCGTGGCCCACCATGCAGGAGTACTGGTCACCCTGTTTCCAGAGTTCAGCTGATAC
ACGCAACACGCTTGTCACCAGGTAGGTGGTGGCTCCCTCGCCTGGCTCCTTTAGGGGCTCAAACACTAGG
TAGCTTTCTGGGGACAGCTCCTCATTTCCATGCAGCCATCGCACCAGCACTTCTTTAGGGTTGAAAGCTC
GCACCAGGCATGTCAGGGACACGAGCTCATTCAGGGCCAGCTCCTCCGACGGCGGCGGTAGCAGGTGGAC
CTGGGGTGGGAAGGTGTTCACTGGAAGGTAGAAAGAAGAGTTATGGCTCAGACAAGGAGAGCAAGACCCC
TCTGCCCTTCCCTTCTGGGCAGTTCCTCAGATAGCTCCAATTTCCCTCTGTGATATAAGGACAGGAGCAG
TTAGTTGAGCATCTGTATTATAGGAAGAAAGCAGGCATGTGAGCAGGGAACGTCATACAATGTCCCGGGT
ATGCATCTGGGCTCACCTGTGATTTTGGCAATTGTGCCAGTTAAGGTGTCAGACTCAGGATGGGTAACTG
TGCACTTGAATGATGCGCCACTGTTCCAGCGCTCAGCACAGCCAGGCAGGACGCTGGACACACTGTAGCA
GCCGCAGGAATTCTGCACAGCTTTCTTCTGCACTGCATCCTTCCCAGTGGAGGGCTCCCAGGTGAAGACA
GCTCCCTCAGGATTTCTCAGGCCATTCAGAGTACATGTGAGGCTGGCATCTGAACCCAGGAGCAGGTCCT
CAAGAGCTGGCCGCTGCAGTGACAGGCTGGGATGGCAGGAAGGAGGACAAGGAGGACAAGGAGGAGGAGG
ACCTGTAACAGAGAAGTCCTAGCAAATCAGTATATTCTCCTTTTCTCCTTTGCTGTCCCCTTTTCTGGAT
GTTCTGAGGCCTCAGTTCCTTATGGCCTGTTTACGCTTCCCTTCCCCCACCCCCATGGGAGGTTTCTGAG
GGTCCCTGTGAGGGTGTGCCTCAGAGGGATATATGGATCTAGATAAGGTAGAACTTATCCCACCCCCAGC
TGACCCCCTAACGTTCTTTACCAGAGCACTTCACATCCAATTCTTGGACGGCGTTAGAGTCATGTTGCAC
GGAACATTTCACGGATTCTCCTTCTGGGCACTCGACAGCTGGCAGGGTCAACTGGCTGCTCATGGTGTAC
CCTCCCCCAGAGGCCAGGGCAGGTGGGAAGTTTACGGTGGTTATATCCTTCCCACTCTTTCCCCAGGTCA
CATTCATCGTGCCGGAAGGGAAGTAATCGTGAATCAGGCAGCCGATTATCACTGGGTCACTTGACAGAGC
TCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGAC
-
Molecule Role :
Vaximmutor
- Related Vaccine(s):
C. muridarum MOMP protein vaccine
|
3. IgG |
-
Gene Name :
IgG
-
Sequence Strain (Species/Organism) :
Mus musculus
-
NCBI Gene ID :
16059
-
Genbank Accession :
AF010213
-
Taxonomy ID :
10090
-
Chromosome No :
12
-
Gene Starting Position :
881411
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Gene Ending Position :
914690
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Gene Strand (Orientation) :
+
-
Protein Name :
immunoglobulin heavy chain (V7183 family)
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Protein Note :
Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)
-
DNA Sequence : Show Sequence
>gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
ACAAGCACAGTGCAAATTTCTGTCACCCTG
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Molecule Role :
Vaximmutor
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Additional Molecule Role :
Vaximmutor
- Related Vaccine(s):
C. muridarum MOMP protein vaccine
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IV. Vaccine Information |
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1. C. muridarum DNA vaccine encoding TC0439 |
a. Vaccine Ontology ID: |
VO_0011533 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Antigen |
C. muridarum TC0439 adherence factor |
e. Gene Engineering of
TC0439 adherence factor |
- Type:
DNA vaccine construction
- Description:
The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
- Detailed Gene Information: Click here.
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f. Vector: |
pCI30 |
g. Immunization Route |
Subcutaneous |
h.
Mouse Response |
- Host Strain:
BALB/c
- Vaccination Protocol:
Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007).
- Challenge Protocol:
All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
- Efficacy:
The groups of female BALB/c mice immunized intra-abdominally with TC0439 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
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2. C. muridarum DNA vaccine encoding TC0512 |
a. Vaccine Ontology ID: |
VO_0011534 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Antigen |
C. muridarum TC0512 outer membrane protein, putative |
e. Gene Engineering of
TC0512 outer membrane protein, putative |
- Type:
DNA vaccine construction
- Description:
The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
- Detailed Gene Information: Click here.
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f. Vector: |
pCI30 (McNeilly et al., 2007) |
g. Immunization Route |
Subcutaneous injection |
h.
Mouse Response |
- Host Strain:
BALB/c
- Vaccination Protocol:
Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007).
- Challenge Protocol:
All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
- Efficacy:
The groups of female BALB/c mice immunized intra-abdominally with TC0512 showed significant levels of protection when compared to negative control animals. At 9 days following challenge TC0512 showed a 73% reduction in the number of recoverable Chlamydia compared with vector only immunized controls (McNeilly et al., 2007).
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3. C. muridarum DNA vaccine encoding TC0757 |
a. Vaccine Ontology ID: |
VO_0011522 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Antigen |
C. muridarum TC0757 |
e. Gene Engineering of
TC0757 hypothetical protein |
- Type:
DNA vaccine construction
- Description:
The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
- Detailed Gene Information: Click here.
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f. Vector: |
pCI30 |
g. Immunization Route |
Subcutaneous injection |
h.
Mouse Response |
- Host Strain:
BALB/c
- Vaccination Protocol:
Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007).
- Challenge Protocol:
All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
- Efficacy:
The groups of female BALB/c mice immunized intra-abdominally with TC0757 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
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4. C. muridarum DNA vaccine encoding TC0767/768 |
a. Vaccine Ontology ID: |
VO_0011524 |
b. Type: |
DNA vaccine |
c. Status: |
Research |
d. Antigen |
C. muridarum TC0767/768 |
e. Gene Engineering of
TC0767 hypothetical protein |
- Type:
DNA vaccine construction
- Description:
The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
- Detailed Gene Information: Click here.
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f. Gene Engineering of
TC0768 hypothetical protein |
- Type:
DNA vaccine construction
- Description:
The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
- Detailed Gene Information: Click here.
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g. Vector: |
pCI30 |
h. Immunization Route |
intra-abdominal injection |
i.
Mouse Response |
- Host Strain:
BALB/c
- Vaccination Protocol:
Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007).
- Challenge Protocol:
All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
- Efficacy:
The groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
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5. C. muridarum MOMP protein vaccine |
a. Vaccine Ontology ID: |
VO_0011453 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Antigen |
C. muridarum major outer membrane protein MOMP |
e. Gene Engineering of
MOMP |
- Type:
Recombinant protein preparation
- Description:
Recombinant maltose binding protein-MOMP (MBP-MOMP) was cloned into the bacterial expression vector pMAL-c2, and used to transform Escherichia coli (DH5α[pMMM3]). The MBP-MOMP was purified by passage through a PD-10 column (Amersham Biosciences, NSW, Australia), followed by a Sephadex G-75 column (Sigma–Aldrich) (Skelding et al., 2006).
- Detailed Gene Information: Click here.
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f. Adjuvant: |
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g. Adjuvant: |
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h. Immunization Route |
Intranasal immunization |
i.
Mouse Response |
- Host Strain:
BALB/c
- Vaccination Protocol:
Groups of five mice were immunized on days 0, 7, 14, and 35 via either the IN or TCI route. One group of five mice were used as a control, receiving no immunization solution. Mice immunized intranasally were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia), and 10 μl of immunization solution containing 100 μg MOMP, admixed with 10 μg CpG-ODN 1826 (5′-TCC ATG ACG TTC CTG ACG TT-3′) (Geneworks, SA, Australia), and 10 μg cholera toxin (Sigma–Aldrich) was gently pipetted onto each nare (5 μl per nare) (Skelding et al., 2006).
- Challenge Protocol:
Five days after the final boost, mice were infected intranasally with C. muridarum. Mice were anaesthetised with 4% isoflurane and 10^3 IFUs were gently pipetted onto the nares. Mice were weighed daily for 10 days then sacrificed 10 days post-infection via a sodium pentobarbital overdose (Nembutal, Abbott Australasia). The lungs from the mice were either perfused and fixed for histopathology or collected for determination of C. muridarum levels (Skelding et al., 2006).
- Efficacy:
Both intranasal and transcutaneous immunization protected mice against respiratory challenge with Chlamydia (Skelding et al., 2006).
- Host Gene Response of
Ifng (Interferon gamma)
- Gene Response:
Both TCI and IN immunization resulted in greatly increased IFN-γ gene expression in stimulated lymph node cells (stimulated with MOMP) as compared to non-immunized mice (Skelding et al., 2006).
- Detailed Gene Information: Click here.
- Host Gene Response of
IgA
- Gene Response:
Both routes of immunization induced a significant MOMP-specific IgA response in serum with the highest IgA response seen in animals immunized intranasally. These results were compared 7 days post-immunization to non-immunized control mice (Skelding et al., 2006).
- Detailed Gene Information: Click here.
- Host Gene Response of
IgG
- Gene Response:
Both routes of immunization (TCI and IN) resulted in levels of MOMP-specific IgG in serum that were significantly higher than that in serum of non-immunized control mice. There was no significant difference between serum IgG levels in animals immunized by either route. These results were gathered 7 days post-immunization (Skelding et al., 2006).
- Detailed Gene Information: Click here.
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6. C. muridarum PmpE/F-2 protein vaccine |
a. Vaccine Ontology ID: |
VO_0011456 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Antigen |
C. muridarum polymorphic membrane protein E/F family protein |
e. Gene Engineering of
PmpE/F-2 |
- Type:
Recombinant protein preparation
- Description:
The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: pmpE/F-2 and gap DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pET32a (Novagen) for pmpE/F-2 and gap after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques. Only the first half of the gene (25–575) was cloned into the vector for expression (Yu et al., 2009).
- Detailed Gene Information: Click here.
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f. Adjuvant: |
- VO ID:
VO_0000142
- Description:
Incomplete Freunds Adjuvant (Sigma)
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g. Immunization Route |
Intravenous injection (i.v.) |
h.
Mouse Response |
- Host Strain:
C57BL/6
- Vaccination Protocol:
Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein PmpE/F-225–575 in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009).
- Challenge Protocol:
Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009).
- Efficacy:
PmpE/F-2(25-575) immunized mice exhibited significant resistance to challenge infection. In addition, pmpE/F-2 was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
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7. C. muridarum PmpG-1 protein vaccine |
a. Vaccine Ontology ID: |
VO_0011458 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Antigen |
C. muridarum polymorphic membrane protein G family protein |
e. Gene Engineering of
PmpG-1 |
- Type:
Recombinant protein preparation
- Description:
The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: pmpG-1 and gap DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pET32a (Novagen) for pmpG-1 and gap after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques. Only the first half of the gene (25–500) was cloned into the vector for expression (Yu et al., 2009).
- Detailed Gene Information: Click here.
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f. Adjuvant: |
- VO ID:
VO_0000142
- Description:
Incomplete Freunds Adjuvant (Sigma)
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g. Immunization Route |
Intravenous injection (i.v.) |
h.
Mouse Response |
- Host Strain:
C57BL/6
- Vaccination Protocol:
Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein PmpG-1 25–500 in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009).
- Challenge Protocol:
Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009).
- Efficacy:
Overall, vaccination with DCs transfected with PmpG-1(25-500) exhibited the greatest degree of protective immunity among the four Chlamydia Ags tested (Yu et al., 2009).
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8. C. muridarum PmpG-1, PmpE/F-2, and MOMP Proteins Vaccine |
a. Vaccine Ontology ID: |
VO_0004257 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Antigen |
Chlamydia proteins PmpG-1, PmpE/F-2, and MOMP (Yu et al., 2010). |
e. Adjuvant: |
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f. Immunization Route |
subcutaneous injection |
g.
Mouse Response |
- Host Strain:
C57BL/6 and BALB/c
- Vaccination Protocol:
Mice were vaccinated 3 times subcutaneously with PmpG-1 plus PmpE/F-2 plus MOMP plus AbISCO-100 (G+F+M+AbISCO) (Yu et al., 2010).
- Challenge Protocol:
Two weeks after the final immunization, the mice in the three animal trials described above were then challenged with live EBs for protection evaluation (Yu et al., 2010).
- Efficacy:
The combination of three Chlamydia proteins formulated with AbISCO exhibited significant protection at day 13 compared to the group with adjuvant alone (Yu et al., 2010).
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9. C. muridarum RplF protein vaccine |
a. Vaccine Ontology ID: |
VO_0011437 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Antigen |
C. muridarum rplF |
e. Gene Engineering of
RplF |
- Type:
Recombinant protein preparation
- Description:
The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: rplF DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pGEX-6P-3 (GE Healthcare) after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques (Yu et al., 2009).
- Detailed Gene Information: Click here.
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f. Adjuvant: |
- VO ID:
VO_0000142
- Description:
Incomplete Freunds Adjuvant (Sigma)
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g. Immunization Route |
Intravenous injection (i.v.) |
h.
Mouse Response |
- Host Strain:
C57BL/6
- Vaccination Protocol:
Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein RplF in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009).
- Challenge Protocol:
Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009).
- Efficacy:
RplF was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
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10. C. muridarum Tarp subunit vaccine |
a. Vaccine Ontology ID: |
VO_0004202 |
b. Type: |
Subunit vaccine |
c. Status: |
Research |
d. Antigen |
purified C. muridarum Tarp |
e. Gene Engineering of
Tarp |
- Type:
Recombinant protein preparation
- Description:
- Detailed Gene Information: Click here.
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f. Adjuvant: |
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g. Adjuvant: |
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h. Immunization Route |
Intramuscular injection (i.m.) |
i.
Mouse Response |
- Host Strain:
BALB/c
- Vaccination Protocol:
Mice were immunized intramuscularly with purified C. muridarum Tarp emulsified in CPG-IFA adjuvant or just adjuvant (as a control). Mice were immunized in the hind legs 3 times with an interval of 3 weeks between the first and second injections and 9 days between the second and third injections (Wang et al., 2009).
- Challenge Protocol:
Thirty days after the third immunization, each mouse was inoculated intravaginally with 1 × 10^4 IFUs of live C. muridarum organisms in 20μl of SPG (sucrose-phosphate-glutamate buffer). Five days prior to infection, each mouse was injected with 2.5mg Depo-provera subcutaneously to synchronize menstrual cycle and increase mouse susceptibility to chlamydial infection (Wang et al., 2009).
- Efficacy:
Immunization of mice with Tarp induced Th1-dominant immunity that significantly reduced the shedding of live organisms from the lower genital tract and attenuated inflammatory pathologies in the fallopian tube tissues. Tarp, an immunodominant antigen identified by human antisera, can induce protective immunity against chlamydial infection and pathology in mice (Wang et al., 2009).
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V. References |
1. Faludi et al., 2009: Faludi I, Burian K, Csanadi A, Miczak A, Lu X, Kakkar VV, Gonczol E, Endresz V. Adjuvant modulation of the immune response of mice against the LcrE protein of Chlamydophila pneumoniae. International journal of medical microbiology : IJMM. 2009; 299(7); 520-528. [PubMed: 19451031].
2. McNeilly et al., 2007: McNeilly CL, Beagley KW, Moore RJ, Haring V, Timms P, Hafner LM. Expression library immunization confers partial protection against Chlamydia muridarum genital infection. Vaccine. 2007; 25(14); 2643-2655. [PubMed: 17239501].
3. Mosolygó et al., 2014: Mosolygó T, Szabó AM, Balogh EP, Faludi I, Virók DP, Endrész V, Samu A, Krenács T, Burián K. Protection promoted by pGP3 or pGP4 against Chlamydia muridarum is mediated by CD4(+) cells in C57BL/6N mice. Vaccine. 2014; 32(40); 5228-5233. [PubMed: 25077421].
4. Skelding et al., 2006: Skelding KA, Hickey DK, Horvat JC, Bao S, Roberts KG, Finnie JM, Hansbro PM, Beagley KW. Comparison of intranasal and transcutaneous immunization for induction of protective immunity against Chlamydia muridarum respiratory tract infection. Vaccine. 2006; 24(3); 355-366. [PubMed: 16153755].
5. Tifrea et al., 2016: Tifrea DF, Barta ML, Pal S, Hefty PS, de la Maza LM. Computational modeling of TC0583 as a putative component of the Chlamydia muridarum V-type ATP synthase complex and assessment of its protective capabilities as a vaccine antigen. Microbes and infection. 2016; 18(4); 245-253. [PubMed: 26706820].
6. Wang et al., 2009: Wang J, Chen L, Chen F, Zhang X, Zhang Y, Baseman J, Perdue S, Yeh IT, Shain R, Holland M, Bailey R, Mabey D, Yu P, Zhong G. A chlamydial type III-secreted effector protein (Tarp) is predominantly recognized by antibodies from humans infected with Chlamydia trachomatis and induces protective immunity against upper genital tract pathologies in mice. Vaccine. 2009; 27(22); 2967-2980. [PubMed: 19428908].
7. Wiki: Chlamydia muridarum: Chlamydia muridarum [http://en.wikipedia.org/wiki/Chlamydia_muridarum]
8. Yu et al., 2009: Yu H, Jiang X, Shen C, Karunakaran KP, Brunham RC. Novel Chlamydia muridarum T cell antigens induce protective immunity against lung and genital tract infection in murine models. Journal of immunology (Baltimore, Md. : 1950). 2009; 182(3); 1602-1608. [PubMed: 19155509].
9. Yu et al., 2009: Yu H, Jiang X, Shen C, Karunakaran KP, Brunham RC. Novel Chlamydia muridarum T cell antigens induce protective immunity against lung and genital tract infection in murine models. Journal of immunology (Baltimore, Md. : 1950). 2009; 182(3); 1602-1608. [PubMed: 19155509].
10. Yu et al., 2010: Yu H, Jiang X, Shen C, Karunakaran KP, Jiang J, Rosin NL, Brunham RC. Chlamydia muridarum T-cell antigens formulated with the adjuvant DDA/TDB induce immunity against infection that correlates with a high frequency of gamma interferon (IFN-gamma)/tumor necrosis factor alpha and IFN-gamma/interleukin-17 double-positive CD4+ T cells. Infection and immunity. 2010; 78(5); 2272-2282. [PubMed: 20231405].
11. Yu et al., 2014: Yu H, Karunakaran KP, Jiang X, Brunham RC. Evaluation of a multisubunit recombinant polymorphic membrane protein and major outer membrane protein T cell vaccine against Chlamydia muridarum genital infection in three strains of mice. Vaccine. 2014; 32(36); 4672-4680. [PubMed: 24992718].
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