VIOLIN: Vaccine Investigation and Online Information Network

Chlamydophila pneumoniae

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. copN (Protective antigen)
2. enolase (Protective antigen)
3. FabD (Protective antigen)
4. GroEL (Protective antigen)
5. LcrE (Protective antigen)
6. major outer membrane protein, MOMP (Protective antigen)
7. OmpH-like outer membrane protein (Protective antigen)
8. PknD (Protective antigen)
9. pmp10 (Protective antigen)
10. pmp2 (Protective antigen)
11. Ssb (Protective antigen)
Vaccine Related Host Genes
1. Ifng (Interferon gamma)
2. IgA
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

83558

2. Disease:

Pneumonia

3. Introduction

Chlamydophila pneumoniae is a species of Chlamydophila bacteria[1][2][3] that infects humans and is a major cause of pneumonia. C. pneumoniae has a complex life cycle and must infect another cell in order to reproduce and thus is classified as an obligate intracellular pathogen. This atypical bacterium commonly causes pharyngitis, bronchitis and atypical pneumonia mainly in elderly and debilitated patients but in healthy adults also. C. pneumoniae infection has been implicated in several chronic lung diseases by serology and direct antigen detection. Acute lower respiratory tract infection caused by C. pneumoniae seems often to precede attacks of asthma in both children and adults but is also involved in some exacerbations of chronic bronchitis. More importantly it seems to be strongly associated with chronic obstructive lung disease irrespective of exacerbation status. Moreover, persistently elevated C. pneumoniae antibody titers have been observed in sarcoidosis and lung cancer (Wiki: Chlamydophila pneumoniae).

4. Microbial Pathogenesis

Chlamydophila pneumoniae is a small bacterium (0.2 to 1 micrometer) that undergoes several transformations during its life cycle. It exists as an elementary body (EB) in between hosts. The EB is not biologically active but is resistant to environmental stresses and can survive outside of a host for a limited time. The EB travels from an infected person to the lungs of a non-infected person in small droplets and is responsible for infection. Once in the lungs, the EB is taken up by cells in a pouch called an endosome by a process called phagocytosis. However, the EB is not destroyed by fusion with lysosomes as is typical for phagocytosed material. Instead, it transforms into a reticulate body and begins to replicate within the endosome. The reticulate bodies must utilize some of the host's cellular machinery to complete its replication. The reticulate bodies then convert back to elementary bodies and are released back into the lung, often after causing the death of the host cell. The EBs are thereafter able to infect new cells, either in the same organism or in a new host. Thus, the life cycle of C. pneumoniae is divided between the elementary body, which is able to infect new hosts but can not replicate, and the reticulate body ,which replicates but is not able to cause new infection (Wiki: Chlamydophila pneumoniae).

5. Host Ranges and Animal Models

In addition to infecting humans, C. pneumoniae also infects and causes disease in Koalas, emerald tree boa (Corallus caninus), iguanas, chameleons, frogs, and turtles (Wiki: Chlamydophila pneumoniae).

II. Vaccine Related Pathogen Genes

1. copN

Gene Name : copN
Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
VO ID : VO_0010884
NCBI Protein GI : 33241669
Other Database IDs : CDD:274206
Taxonomy ID : 182082
Gene Strand (Orientation) : ?
Protein Name : CopN
Protein pI : 4.72
Protein Weight : 42139.89
Protein Length : 458
Protein Note : type strain

Protein Sequence : Show Sequence

>NP_876610.1 CopN [Chlamydophila pneumoniae TW-183]
MAASGGTGGLGGTQGVNLAAVEAAAAKADAAEVVASQEGSEMNMIQQSQDLTNPAAATRTKKKEEKFQTL
ESRKKGEAGKAEKKSESTEEKPDTDLADKYASGNSEISGQELRGLRDAIGDDASPEDILALVQEKIKDPA
LQSTALDYLVQTTPPSQGKLKEALIQARNTHTEQFGRTAIGAKNILFASQEYADQLNVSPSGLRSLYLEV
TGDTHTCDQLLSMLQDRYTYQDMAIVSSFLMKGMATELKRQGPYVPSAQLQVLMTETRNLQAVLTSYDYF
ESRVPILLDSLKAEGIQTPSDLNFVKVAESYHKIINDKFPTASKVEREVRNLIGDDVDSVTGVLNLFFSA
LRQTSSRLFSSADKRQQLGAMIANALDAVNINNEDYPKASDFPKPYPWS

Molecule Role : Protective antigen
Molecule Role Annotation : Results of this study showed that intranasal immunization of BALB/c mice with heat-aggregated CopN protein and an Escherichia coli heat-labile toxin (LT) induced a strong immune response. The immunization induced statistically significant protection against intranasal C. pneumoniae challenge, the level of which correlated with the magnitude of CopN-specific lymphocyte proliferation (Tammiruusu et al., 2007).
Related Vaccine(s): C. pneumoniae CopN protein vaccine

2. enolase

Gene Name : enolase
Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
NCBI Protein GI : AAP98758
Other Database IDs : CDD:213580
CDD:239429
Taxonomy ID : 182082
Protein Name : enolase
Protein pI : 4.37
Protein Weight : 44335.42
Protein Length : 486
Protein Note : type strain of Chlamydophila pneumoniae;
biovar: TWAR

Protein Sequence : Show Sequence

>AAP98758.1 enolase [Chlamydia pneumoniae TW-183]
MFEAVIADIQAREILDSRGYPTLHVKVTTSTGSVGEARVPSGASTGKKEALEFRDTDSPRYQGKGVLQAV
KNVKEILFPLVKGCSVYEQSLIDSLMMDSDGSPNKETLGANAILGVSLATAHAAAATLRRPLYRYLGGCF
ACSLPCPMMNLINGGMHADNGLEFQEFMIRPIGASSIKEAVNMGADVFHTLKKLLHERGLSTGVGDEGGF
APNLASNEEALELLLLAIEKAGFTPGKDISLALDCAASSFYNVKTGTYDGRHYEEQIAILSNLCDRYPID
SIEDGLAEEDYDGWALLTEVLGEKVQIVGDDLFVTNPELILEGISNGLANSVLIKPNQIGTLTETVYAIK
LAQMAGYTTIISHRSGETTDTTIADLAVAFNAGQIKTGSLSRSERVAKYNRLMEIEEELGSEAIFTDSNV
FAYEDSEE

Molecule Role : Protective antigen
Molecule Role Annotation : Four of the six in vitro neutralizing antigens (Pmp2, Pmp10, OmpH-like and enolase) could inhibit Cpn dissemination in a hamster model. The results show that these Cpn proteins are immunoaccessible in infectious EBs, and recommend further investigation on their value as vaccine components.(Finco et al., 2005)

3. FabD

Gene Name : FabD
Sequence Strain (Species/Organism) : Chlamydophila pneumoniae CWL029
VO ID : VO_0010895
NCBI Gene ID : 894994
NCBI Protein GI : 15618217
Locus Tag : CPn0297
Genbank Accession : AE001363
Protein Accession : NP_224502
Taxonomy ID : 115713
Gene Starting Position : 334770
Gene Ending Position : 335696
Gene Strand (Orientation) : -
Protein Name : malonyl CoA-acyl carrier protein transacylase
Protein pI : 4.92
Protein Weight : 31463.85
Protein Length : 308

DNA Sequence : Show Sequence

>NC_000922.1:334770-335696 Chlamydophila pneumoniae CWL029 chromosome, complete genome
ATCATACCTCTGATAGGAATTTTTCAATCTGAGCAAAAGTACCAAGACTTGTAATCGGTTTAGAAATCCC
TATAGAGCGATTTAAACCAGCCAAAACTTTTCCTGGACCTAATTCTAAAAACTCATCCACCTCTGATTCG
ATATGGTAACAACTCTGATACCATAACGTAGGTGATGTCATTTGCCGAGCTAAACACTCTCGCATTTCTT
CAGTATTTACTAAAGATTTTCCTACCACGTGTGACACTAAGGGAAGGCTAGAATCTTTCATGCATAAAGC
ATAAATGTCTGGAGCTAAGCCATCTTGAGCAACTTGCATTAAAGGAGTATGAAATGCTCCAGACACCTTT
AAACGAACTGCTTTTTTACATCCTAAATCACGAAATAACTCAATCGCTTGGTCTACTTTTTCTGCTATTC
CAGCCACTACAAGCTGTTTGGGTGCATTATAATTAGCAATCCAAATTCCTTGACCAAGACTTGTTATATT
TTCCTCTATAACTTCAGAGGGAAGCCCTAATAAAGCCGCCATAGCCCCTGGGCTCTGATTACAAGCTTCA
TTCATTAACTGACCACGCTTTCTAACAAGCTCAAGGCCGTCGAGCACGGAGATTCTATCGGAAGCAACTA
AAGCAGTATACTCCCCTAAACTTAATCCAGAGACTAAAGAAGGCTGAATAGAAGAACGCTGAGATAGAAC
CTTTACCACAGCCATGCTATGAAGATAAATAGCTAGCTGACTATGTACTGTTTCCATCAAAAGATCCTCA
GGACCTTCAAACATAATTGAAGTCAGAGAAAATCCTAACCTTTCATTAGCAAAATCAAAAAGCTCTCTAA
CCTCAGGATACTCCATATATAGGTCTTGTCCCATACCTACATATTGGCTCCCTTGTCCTGGGAACAAAAA
AGCATAACGTTTTTTCA

Protein Sequence : Show Sequence

>NP_224502.1 malonyl CoA-acyl carrier protein transacylase [Chlamydia pneumoniae CWL029]
MKKRYAFLFPGQGSQYVGMGQDLYMEYPEVRELFDFANERLGFSLTSIMFEGPEDLLMETVHSQLAIYLH
SMAVVKVLSQRSSIQPSLVSGLSLGEYTALVASDRISVLDGLELVRKRGQLMNEACNQSPGAMAALLGLP
SEVIEENITSLGQGIWIANYNAPKQLVVAGIAEKVDQAIELFRDLGCKKAVRLKVSGAFHTPLMQVAQDG
LAPDIYALCMKDSSLPLVSHVVGKSLVNTEEMRECLARQMTSPTLWYQSCYHIESEVDEFLELGPGKVLA
GLNRSIGISKPITSLGTFAQIEKFLSEV

Molecule Role : Protective antigen
Molecule Role Annotation : M-ID vaccination with fabD generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of fabD to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
Related Vaccine(s): C. pneumoniae DNA vaccine encoding FabD

4. GroEL

Gene Name : GroEL
Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
NCBI Protein GI : WP_010883533
Other Database IDs : CDD:223535
CDD:295468
Taxonomy ID : 83558
Gene Strand (Orientation) : ?
Protein Name : chaperonin GroEL
Protein pI : 5.03
Protein Weight : 54952.93
Protein Length : 584
Protein Note : Chaperonin GroEL (HSP60 family) [Posttranslational modification, protein turnover, chaperones]; COG0459

Protein Sequence : Show Sequence

>WP_010883533.1 chaperonin GroEL [Chlamydia pneumoniae]
MSEQEKLSNYNADKKLFSGIDKLFQIVKGSYGPKQSLSPTSFFKERGFYAISQTELSNSYENLGVDFAKA
MVNKIHKEHSDGATTGLILLHAILQESYAALEKGISTHKLIASLKLQGEKLQEALQQQSWPIKDALKVRN
IIFSSLHMPTIADHFYNAFSVVGPEGLISITKERENDKTSMDVFQGFKIPAGYASTYFVSDTASRLTRIA
HPLILITDRKISMIHSLLPLLQEISEQNQHLIIFCEDIDPDVLATLVVNKLQGLLQVTVVTIPQLSTTNQ
ELAEDIALFTGTHICPCQEASHVLAPEMVTLGSCLSIEISESQTTLIGGLHIPEVLTLKTRQLAEEIRTT
SCLETKKRLIKSTNRLQSSVAILPTDEDNEPLYTLALKIMESALSRGYVPGGGVALFYASLTLGTPKDDA
DENSIAISLLQKACCAPLKLLATNADLDGDAVIAKLSSLGTTSLGISVFSREIEDLIAGGILDSLATTST
ILAQALDTAILVLSSKILILENQYEISTL

Molecule Role : Protective antigen
Molecule Role Annotation : Immunization of mice with a single construct containing multiple epitopes derived from ApoB100, hHSP60 and Cpn was more effective in reducing early atherosclerotic lesions.(Lu et al., 2012)

Immunization with pmomp or phsp60 showed 1.2–1.5 log reduction in the mean lung bacterial counts after the challenge.(Penttilä et al., 2000)
Related Vaccine(s): C. pneumoniae DNA vaccine pHSP-60

5. LcrE

Gene Name : LcrE
Sequence Strain (Species/Organism) : Chlamydophila pneumoniae CWL029
VO ID : VO_0010883
NCBI Gene ID : 895078
NCBI Protein GI : 15618244
Locus Tag : CPn0324
Genbank Accession : AE001363
Protein Accession : NP_224529
Taxonomy ID : 115713
Gene Starting Position : 369491
Gene Ending Position : 370690
Gene Strand (Orientation) : +
Protein Name : low calcium response protein E
Protein pI : 4.72
Protein Weight : 41321.12
Protein Length : 399

DNA Sequence : Show Sequence

>NC_000922.1:369491-370690 Chlamydophila pneumoniae CWL029 chromosome, complete genome
TATGGCAGCATCAGGAGGCACAGGTGGTTTAGGAGGCACTCAGGGTGTCAACCTTGCAGCTGTAGAAGCT
GCAGCTGCAAAAGCAGATGCAGCAGAAGTTGTAGCCAGCCAAGAAGGTTCTGAGATGAACATGATTCAAC
AATCTCAGGACCTGACAAATCCCGCAGCAGCAACACGCACGAAAAAAAAGGAAGAGAAGTTTCAAACTCT
AGAATCTCGGAAAAAAGGAGAAGCTGGAAAGGCTGAGAAAAAATCTGAATCTACAGAAGAGAAGCCTGAC
ACAGATCTTGCTGATAAGTATGCTTCTGGGAATTCTGAAATCTCTGGTCAAGAACTTCGCGGCCTGCGTG
ATGCAATAGGAGACGATGCTTCTCCAGAAGACATTCTTGCTCTTGTACAAGAGAAAATTAAAGACCCAGC
TCTGCAATCCACAGCTTTGGACTACCTGGTTCAAACGACTCCACCCTCCCAAGGTAAATTAAAAGAAGCG
CTTATCCAAGCAAGGAATACTCATACGGAGCAATTCGGACGAACTGCTATTGGTGCGAAAAACATCTTAT
TTGCCTCTCAAGAATATGCAGACCAACTGAATGTTTCTCCTTCAGGGCTTCGCTCTTTGTACTTAGAAGT
GACTGGAGACACACATACCTGTGATCAGCTACTTTCTATGCTTCAAGACCGCTATACCTACCAAGATATG
GCTATTGTCAGCTCCTTTCTAATGAAAGGAATGGCAACAGAATTAAAAAGGCAGGGTCCCTACGTACCCA
GTGCGCAACTACAAGTTCTCATGACAGAAACTCGTAACCTGCAAGCAGTTCTTACCTCGTACGATTACTT
TGAAAGTCGCGTTCCTATTTTACTCGATAGCTTAAAAGCTGAGGGAATCCAAACTCCTTCTGATCTAAAC
TTTGTGAAGGTAGCTGAGTCCTACCATAAAATCATTAACGATAAGTTCCCAACAGCATCTAAAGTAGAAC
GAGAAGTCCGCAATCTCATAGGAGACGATGTTGATTCTGTGACCGGTGTCTTGAACTTATTCTTTTCTGC
TTTACGTCAAACGTCGTCACGCCTTTTCTCTTCAGCAGACAAACGTCAGCAATTAGGAGCTATGATTGCT
AATGCTTTAGATGCTGTAAATATAAACAATGAAGATTATCCCAAAGCATCAGACTTCCCTAAACCCTATC
CTTGGTCATG

Protein Sequence : Show Sequence

>NP_224529.1 low calcium response protein E [Chlamydia pneumoniae CWL029]
MAASGGTGGLGGTQGVNLAAVEAAAAKADAAEVVASQEGSEMNMIQQSQDLTNPAAATRTKKKEEKFQTL
ESRKKGEAGKAEKKSESTEEKPDTDLADKYASGNSEISGQELRGLRDAIGDDASPEDILALVQEKIKDPA
LQSTALDYLVQTTPPSQGKLKEALIQARNTHTEQFGRTAIGAKNILFASQEYADQLNVSPSGLRSLYLEV
TGDTHTCDQLLSMLQDRYTYQDMAIVSSFLMKGMATELKRQGPYVPSAQLQVLMTETRNLQAVLTSYDYF
ESRVPILLDSLKAEGIQTPSDLNFVKVAESYHKIINDKFPTASKVEREVRNLIGDDVDSVTGVLNLFFSA
LRQTSSRLFSSADKRQQLGAMIANALDAVNINNEDYPKASDFPKPYPWS

Molecule Role : Protective antigen
Molecule Role Annotation : The immunogenicity and protective effect of recombinant LcrE protein combined either with Freund's or Alum adjuvant were investigated in mice. The immunization with both protocols resulted in a significant reduction of the number of viable C. pneumoniae in the lungs after challenge. Results confirm that LcrE induces protective immunity in mice (Faludi et al., 2009).

DNA immunization given as a priming and followed by a protein booster significantly reduced the number of viable bacteria in the lungs after challenge with C. pneumoniae. These results confirm that immunization with pΔRCLcrE can be an effective part of a vaccination schedule against C. pneumoniae. (Faludi and Szab�, 2011)
Related Vaccine(s): C. pneumoniae LcrE protein vaccine

6. major outer membrane protein, MOMP

Gene Name : major outer membrane protein, MOMP
Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
NCBI Protein GI : WP_010883333
Other Database IDs : CDD:279628
Taxonomy ID : 83558
Gene Strand (Orientation) : ?
Protein Name : porin
Protein pI : 7.61
Protein Weight : 39227.66
Protein Length : 441
Protein Note : Chlamydia major outer membrane protein; pfam01308

Protein Sequence : Show Sequence

>WP_010883333.1 porin [Chlamydia pneumoniae]
MKKLLKSALLSAAFAGSVGSLQALPVGNPSDPSLLIDGTIWEGAAGDPCDPCATWCDAISLRAGFYGDYV
FDRILKVDAPKTFSMGAKPTGSAAANYTTAVDRPNPAYNKHLHDAEWFTNAGFIALNIWDRFDVFCTLGA
SNGYIRGNSTAFNLVGLFGVKGTTVNANELPNVSLSNGVVELYTDTSFSWSVGARGALWECGCATLGAEF
QYAQSKPKVEELNVICNVSQFSVNKPKGYKGVAFPLPTDAGVATATGTKSATINYHEWQVGASLSYRLNS
LVPYIGVQWSRATFDADNIRIAQPKLPTAVLNLTAWNPSLLGNATALSTTDSFSDFMQIVSCQINKFKSR
KACGVTVGATLVDADKWSLTAEARLINERAAHVSGQFRF

Molecule Role : Protective antigen
Molecule Role Annotation : The use of MOMP in C. pneumoniae as a possible vaccine target and the role of MOMP-derived peptides as vaccine candidates for immune-therapy in chronic inflammation.(Atanu et al., 2013)

7. OmpH-like outer membrane protein

Gene Name : OmpH-like outer membrane protein
Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
NCBI Protein GI : AAP98243
Other Database IDs : CDD:225381
Taxonomy ID : 182082
Protein Name : OmpH-like outer membrane protein
Protein pI : 4.77
Protein Weight : 19553.64
Protein Length : 250
Protein Note : type strain of Chlamydophila pneumoniae;
biovar: TWAR

Protein Sequence : Show Sequence

>AAP98243.1 OmpH-like outer membrane protein [Chlamydia pneumoniae TW-183]
MKKLLFSTFLLVLGSTSAAHANLGYVNLKRCLEESDLGKKETEELEAMKQQFVKNAEKIEEELTSIYNKL
QDEDYMESLSDSASEELRKKFEDLSGEYNAYQSQYYQSINQSNVKRIQKLIQEVKIAAESVRSKEKLEAI
LNEEAVLAIAPGTDKTTEIIAILNESFKKQN

Molecule Role : Protective antigen
Molecule Role Annotation : Four of the six in vitro neutralizing antigens (Pmp2, Pmp10, OmpH-like and enolase) could inhibit Cpn dissemination in a hamster model. The results show that these Cpn proteins are immunoaccessible in infectious EBs, and recommend further investigation on their value as vaccine components.(Finco et al., 2005)

8. PknD

Gene Name : PknD
Sequence Strain (Species/Organism) : Chlamydophila pneumoniae CWL029
VO ID : VO_0010894
NCBI Gene ID : 895704
NCBI Protein GI : 161353778
Locus Tag : CPn0095
Genbank Accession : AE001363
Protein Accession : NP_224303
Taxonomy ID : 115713
Gene Starting Position : 115994
Gene Ending Position : 118792
Gene Strand (Orientation) : +
Protein Name : serine/threonine protein kinase
Protein pI : 6.03
Protein Weight : 102446.37
Protein Length : 932
Protein Note : PknD; responsible for phosphorylation of proteins on serine and threonine residues; similar to eukaryotic Ser/Thr kinases; in Chlamydia trachomatis itseems to interact with Pkn1, another serine/threonine-protein kinase

DNA Sequence : Show Sequence

>NC_000922.1:115994-118792 Chlamydophila pneumoniae CWL029 chromosome, complete genome
TTTGGAGCGCTATGATATTGTTAGAATTATTGGAAAGGGAGGCATGGGTGAAGTCTATCTTGCCTACGAT
CCTGTATGTTCTCGTAAAGTAGCTCTTAAAAAAATTCGTGAAGATCTTGCAGAAAATCCTCTTTTGAAAA
GGAGGTTTTTACGAGAGGCAAGAATTGCCGCTGACCTTATTCATCCTGGTGTTGTTCCTGTCTATACTAT
TTACAGCGAGAAAGATCCTGTATACTACACGATGCCCTACATAGAGGGATATACACTAAAAACCTTACTG
AAGAGTGTATGGCAAAAGGAATCCCTGTCTAAGGAATTAGCAGAGAAAACTTCTGTAGGGGCATTTCTTT
CTATCTTTCATAAGATCTGCTGCACTATAGAATATGTCCATTCTCGGGGCATTCTTCATCGCGACCTTAA
ACCCGATAACATCTTATTAGGTCTTTTTAGTGAGGCTGTAATCTTAGATTGGGGAGCAGCAGTTGCCTGT
GGAGAAGAAGAGGATCTTCTTGATATAGATGTCAGCAAAGAGGAGGTGCTCTCTTCAAGAATGACAATTC
CAGGAAGAATAGTAGGGACTCCAGATTATATGGCTCCTGAGAGGCTCCTGGGCCATCCAGCTTCTAAAAG
TACAGACATTTATGCTTTAGGAGTGGTTCTTTATCAGATGCTCACTCTCTCTTTTCCTTATAGAAGAAAA
AAAGGAAAGAAAATAGTTCTTGACGGTCAGAGAATTCCAAGTCCTCAAGAGGTAGCTCCTTATCGAGAAA
TCCCTCCGTTTCTTTCCGCTGTAGTGATGAGAATGTTGGCTGTAGATCCTCAAGAGCGCTATTCTTCGGT
AACAGAGCTTAAGGAAGATATCGAGAGTCATCTGAAAGGGAGTCCTAAATGGACTTTAACCACAGCCCTG
CCACCTAAAAAATCTTCTAGTTGGAAGCTAAACGAACCTATTTTACTTTCTAAGTATTTTCCAATGTTGG
AGGTCTCTCCAGCGTCATGGTACAGTTTAGCAATCTCTAATATTGAGAGTTTTTCTGAGATGCGCTTGGA
GTATACTCTTTCTAAAAAAGGCTTGAACGAAGGCTTTGGTATTTTACTTCCCACGTCAGAAAATGCTTTA
GGGGGAGATTTTTACCAGGGGTATGGCTTTTGGCTGCATATTAAGGAGAGAACCTTATCCGTGTCTCTGG
TGAAAAATAGCCTAGAAATCCAGAGGTGCTCTCAAGATTTGGAATCTGATAAAGAGACCTTCTTGATAGC
TTTAGAGCAGCATAATCATAGTTTATCTTTGTTTGTCGATGGTACGACTTGGCTTATCCATATGAATTAT
CTGCCAAGTCGTAGTGGGCGAGTCGCTATCATAGTTCGCGATATGGAAGATATCCTGGAAGATATAGGCA
TTTTTGAAAGTAGTGGCTCTTTGAGGGTCAGTTGTCTTGCTGTTCCTGACGCTTTTCTTGCTGAGAAGTT
ATATGATCGCGCTTTAGTGCTTTACCGAAGGATCGCAGAATCTTTCCCAGGACGTAAAGAAGGTTATGAA
GCAAGGTTCAGAGCAGGAATTACAGTTTTAGAGAAGGCCTCTACAGATAATAATGAACAGGAATTTGCTC
TAGCCATTGAAGAATTCTCAAAATTACATGACGGGGTTGCTGCTCCCTTAGAATACCTTGGTAAGGCTTT
AGTATATCAGAGACTCCAAGAGTATAATGAAGAAATTAAGAGTTTGCTATTAGCATTGAAACGTTATTCG
CAGCATCCTGAAATCTTTAGGCTTAAAGACCATGTGGTTTACCGACTCCATGAGAGCTTTTATAAACGGG
ATCGCCTTGCTCTGGTGTTCATGATTTTAGTATTGGAAATAGCTCCCCAGGCAATCACTCCAGGGCAGGA
AGAAAAAATCCTGGTTTGGTTAAAGGACAAATCTCGGGCTACCTTATTTTGCCTCCTGGATCCCACGGTC
TTAGAGCTGCGCTCTTCTAAAATGGAATTATTTTTAAGTTATTGGTCTGGGTTTATTCCCCATCTCAATA
GTCTATTTCATAGAGCTTGGGATCAAAGCGATGTGCGAGCTTTGATCGAGATTTTCTATGTTGCTTGTGA
TCTTCATAAATGGCAGTTTCTCTCTTCTTGTATCGACATATTTAAAGAGTCTCTTGAGGATCAGAAAGCC
ACAGAAGAGATTGTTGAGTTCTCTTTCGAGGATTTAGGGGCATTTCTTTTTGCTATTCAGAGCATCTTTA
ACAAGGAAGATGCAGAGAAGATCTTTGTTTCTAATGATCAATTATCGCCAATCCTTCTTGTTTATATATT
CGATCTTTTTGCAAATCGTGCTCTTCTGGAATCTCAAGGAGAGGCTATTTTTCAGGCTTTGGATCTCATC
CGAAGTAAAGTTCCTGAAAATTTTTATCATGATTACTTGCGGAATCATGAAATCCGAGCGCATCTTTGGT
GCCGCAATGAGAAGGCTCTAAGCACGATTTTTGAAAACTATACAGAGAAACAGCTAAAGGATGAGCAACA
TGAACTGTTCGTTCTCTATGGATGTTACCTTGCTCTTATACAAGGTGCTGAGGCGGCAAAGCAGCATTTT
GATGTATGTCGTGAAGATCGCATTTTCCCTGCTTCATTATTAGCTAGAAATTACAATCGTTTAGGTCTTC
CCAAAGATGCTCTTAGCTATCAAGAGCGGCGTTTGTTATTGCGACAAAAGTTTCTCTATTTCCATTGTCT
TGGTAACCACGACGAGCGTGACTTATGCCAGACTATGTATCACCTCTTAACCGAAGAATTTCAGCTTTA

Protein Sequence : Show Sequence

>NP_224303.2 serine/threonine protein kinase [Chlamydia pneumoniae CWL029]
MERYDIVRIIGKGGMGEVYLAYDPVCSRKVALKKIREDLAENPLLKRRFLREARIAADLIHPGVVPVYTI
YSEKDPVYYTMPYIEGYTLKTLLKSVWQKESLSKELAEKTSVGAFLSIFHKICCTIEYVHSRGILHRDLK
PDNILLGLFSEAVILDWGAAVACGEEEDLLDIDVSKEEVLSSRMTIPGRIVGTPDYMAPERLLGHPASKS
TDIYALGVVLYQMLTLSFPYRRKKGKKIVLDGQRIPSPQEVAPYREIPPFLSAVVMRMLAVDPQERYSSV
TELKEDIESHLKGSPKWTLTTALPPKKSSSWKLNEPILLSKYFPMLEVSPASWYSLAISNIESFSEMRLE
YTLSKKGLNEGFGILLPTSENALGGDFYQGYGFWLHIKERTLSVSLVKNSLEIQRCSQDLESDKETFLIA
LEQHNHSLSLFVDGTTWLIHMNYLPSRSGRVAIIVRDMEDILEDIGIFESSGSLRVSCLAVPDAFLAEKL
YDRALVLYRRIAESFPGRKEGYEARFRAGITVLEKASTDNNEQEFALAIEEFSKLHDGVAAPLEYLGKAL
VYQRLQEYNEEIKSLLLALKRYSQHPEIFRLKDHVVYRLHESFYKRDRLALVFMILVLEIAPQAITPGQE
EKILVWLKDKSRATLFCLLDPTVLELRSSKMELFLSYWSGFIPHLNSLFHRAWDQSDVRALIEIFYVACD
LHKWQFLSSCIDIFKESLEDQKATEEIVEFSFEDLGAFLFAIQSIFNKEDAEKIFVSNDQLSPILLVYIF
DLFANRALLESQGEAIFQALDLIRSKVPENFYHDYLRNHEIRAHLWCRNEKALSTIFENYTEKQLKDEQH
ELFVLYGCYLALIQGAEAAKQHFDVCREDRIFPASLLARNYNRLGLPKDALSYQERRLLLRQKFLYFHCL
GNHDERDLCQTMYHLLTEEFQL

Molecule Role : Protective antigen
Molecule Role Annotation : IM-ID vaccination with CPn0095 (pknD) generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of CPN0095 to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
Related Vaccine(s): C. pneumoniae DNA vaccine encoding PknD

9. pmp10

Gene Name : pmp10
Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
NCBI Protein GI : CRI40334
Other Database IDs : CDD:273587
CDD:284879
CDD:214872
Taxonomy ID : 83558
Gene Strand (Orientation) : ?
Protein Name : Probable outer membrane protein pmp10
Protein pI : 5.21
Protein Weight : 93433.7
Protein Length : 1016
Protein Note : Chlamydial polymorphic outer membrane protein repeat; TIGR01376

Protein Sequence : Show Sequence

>CRI40334.1 Probable outer membrane protein pmp10 [Chlamydia pneumoniae]
MKSQFSWLVLSSTLACFTSCSTVFAATAENIGPSDSFDGSTNTGTYTPKNTTTGIDYTLTGDITLQNLGD
SAALTKGCFSDTTESLSFAGKGYSLSFLNIKSSAEGAALSVTTDKNLSLTGFSSLTFLAAPSSVITTPSG
KGAVKCGGDLTFDNNGTILFKQDYCEENGGAISTKNLSLKNSTGSISFEGNKSSATGKKGGAICATGTVD
ITNNTAPTLFSNNIAEAAGGAINSTGNCTITGNTSLVFSENSVTATAGNGGALSGDADVTISGNQSVTFS
GNQAVANGGAIYAKKLTLASGGGGGISFSNNIVQGTTAGNGGAISILAAGECSLSAEAGDITFNGNAIVA
TTPQTTKRNSIDIGSTAKITNLRAISGHSIFFYDPITANTAADSTDTLNLNKADAGNSTDYSGSIVFSGE
KLSEDEAKVADNLTSTLKQPVTLTAGNLVLKRGVTLDTKGFTQTAGSSVIMDAGTTLKASTEEVTLTGLS
IPVDSLGEGKKVVIAASAASKNVALSGPILLLDNQGNAYENHDLGKTQDFSFVQLSALGTATTTDVPAVP
TVATPTHYGYQGTWGMTWVDDTASTPKTKTATLAWTNTGYLPNPERQGPLVPNSLWGSFSDIQAIQGVIE
RSALTLCSDRGFWAAGVANFLDKDKKGEKRKYRHKSGGYAIGGAAQTCSENLISFAFCQLFGSDKDFLVA
KNHTDTYAGAFYIQHITECSGFIGCLLDKLPGSWSHKPLVLEGQLAYSHVSNDLKTKYTAYPEVKGSWGN
NAFNMMLGASSHSYPEYLHCFDTYAPYIKLNLTYIRQDSFSEKGTEGRSFDDSNLFNLSLPIGVKFEKFS
DCNDFSYDLTLSYVPDLIRNDPKCTTALVISGASWETYANNLARQALQVRAGSHYAFSPMFEVLGQFVFE
VRGSSRIYNVDLGGKFQF

Molecule Role : Protective antigen
Molecule Role Annotation : Four of the six in vitro neutralizing antigens (Pmp2, Pmp10, OmpH-like and enolase) could inhibit Cpn dissemination in a hamster model. The results show that these Cpn proteins are immunoaccessible in infectious EBs, and recommend further investigation on their value as vaccine components.(Finco et al., 2005)

10. pmp2

Gene Name : pmp2
Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
NCBI Protein GI : CRI32508
Other Database IDs : CDD:273587
CDD:284879
CDD:214872
Taxonomy ID : 83558
Gene Strand (Orientation) : ?
Protein Name : Probable outer membrane protein pmp2
Protein pI : 6.52
Protein Weight : 85607.4
Protein Length : 927
Protein Note : Chlamydial polymorphic outer membrane protein repeat; TIGR01376

Protein Sequence : Show Sequence

>CRI32508.1 Probable outer membrane protein pmp2 [Chlamydia pneumoniae]
MKIPLRFLLISLVPTLSMSNLLGAATTEELSASNSFDGTTSTTSFSSKTSSATDGTNYVFKDSVVIENVP
KTGETQSTSCFKNDAAAGDLNFLGGGFSFTFSNIDATTASGAAIGSEAANKTVTLSGFSALSFLKSPAST
VTNGLGAINVKGNLSLLDNDKVLIQDNFSTGDGGAINCAGSLKIANNKSLSFIGNSSSTRGGAIHTKNLT
LSSGGETLFQGNTAPTAAGKGGAIAIADSGTLSISGDSGDIIFEGNTIGATGTVSHSAIDLGTSAKITAL
RAAQGHTIYFYDPITVTGSTSVADALNINSPDTGDNKEYTGTIVFSGEKLTEAEAKDEKNRTSKLLQNVA
FKNGTVVLKGDVVLSANGFSQDANSKLIMDLGTSLVANTESIELTNLEINIDSLRNGKKIKLSAATAQKD
IRIDRPVVLAISDESFYQNGFLNEDHSYDGILELDAGKDIVISADSRSIDAVQSPYGYQGKWTINWSTDD
KKATVSWAKQSFNPTAEQEAPLVPNLLWGSFIDVRSFQNFIELGTEGAPYEKRFWVAGISNVLHRSGREN
QRKFRHVSGGAVVGASTRMPGGDTLSLGFAQLFARDKDYFMNTNFAKTYAGSLRLQHDASLYSVVSILLG
EGGLREILLPYVSKTLPCSFYGQLSYGHTDHRMKTESLPPPPPTLSTDHTSWGGYVWAGELGTRVAVENT
SGRGFFQEYTPFVKVQAVYARQDSFVELGAISRDFSDSHLYNLAIPLGIKLEKRFAEQYYHVVAMYSPDV
CRSNPKCTTTLLSNQGSWKTKGSNLARQAGIVQASGFRSLGAAAELFGNFGFEWRGSSRSYNVDAGSKIK
F

Molecule Role : Protective antigen
Molecule Role Annotation : Four of the six in vitro neutralizing antigens (Pmp2, Pmp10, OmpH-like and enolase) could inhibit Cpn dissemination in a hamster model. The results show that these Cpn proteins are immunoaccessible in infectious EBs, and recommend further investigation on their value as vaccine components.(Finco et al., 2005)

11. Ssb

Gene Name : Ssb
Sequence Strain (Species/Organism) : Chlamydophila pneumoniae CWL029
VO ID : VO_0010896
NCBI Gene ID : 894901
NCBI Protein GI : 15618301
Locus Tag : CPn0386
Genbank Accession : AE001363
Protein Accession : NP_224586
Taxonomy ID : 115713
Gene Starting Position : 434042
Gene Ending Position : 434524
Gene Strand (Orientation) : -
Protein Name : single-stranded DNA-binding protein
Protein pI : 5.29
Protein Weight : 16434.22
Protein Length : 160
Protein Note : binds to single stranded DNA and may facilitate the binding and interaction of other proteins to DNA

DNA Sequence : Show Sequence

>NC_000922.1:434042-434524 Chlamydophila pneumoniae CWL029 chromosome, complete genome
ATTAAAAAGGAACATCTTCACAGACATACTGCTGTTCTTGACCATAACCAGCATACATATCTTTATCTTT
AATAGCTTCTGCGTCCAGTGCTTCACCTTCAAACCCTACGGATACAGATTCATATCCCACTTGCTGATGA
TTGTCTTCTAAAGATGGAGAACGGCTGCCTTCATTGCGACCGAAAGGACTGAATTTCAAAGAATCTACAC
TAATCACTAAAGAAGATTGCGGTGAACCATCTTTGCTCATGTAACTCTCTACAGAGATATCGCCAGCAAC
AATGACTCCTGAGCCTTTCTTCAAGTAAGGAAGCATCTTATCATAGCGATTGTGCCAAATATTGCATTTG
CACCAAACAGTTTCATCTTTCATTCCAACTCGAGTCTTCACTCCCAGTCTCAGAGTGATCACACGTTTTC
CTTTGGAAGTCATTCGCTCTTCAGGATCTGCTCCAAGGTAACCAGCAAAATGCCCAAACATCA

Protein Sequence : Show Sequence

>NP_224586.1 single-stranded DNA-binding protein [Chlamydia pneumoniae CWL029]
MMFGHFAGYLGADPEERMTSKGKRVITLRLGVKTRVGMKDETVWCKCNIWHNRYDKMLPYLKKGSGVIVA
GDISVESYMSKDGSPQSSLVISVDSLKFSPFGRNEGSRSPSLEDNHQQVGYESVSVGFEGEALDAEAIKD
KDMYAGYGQEQQYVCEDVPF

Molecule Role : Protective antigen
Molecule Role Annotation : Mice vaccinated with candidate gene ssb showed significant reduction of spleen chlamydial loads as compared to naïve, non-protected control mice (p ≤ 0.048). This resulted in the ability of ssb to mediate a modest, but significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
Related Vaccine(s): C. pneumoniae DNA vaccine encoding Ssb

III. Vaccine Related Host Genes

1. Ifng (Interferon gamma)

Gene Name : Ifng (Interferon gamma)
Sequence Strain (Species/Organism) : Mouse
NCBI Gene ID : 15978
NCBI Protein GI : 33468859
Genbank Accession : NM_008337
Protein Accession : NP_032363.1
Other Database IDs : MGI:107656; UniProt: P01580
Taxonomy ID : 10090
Gene Strand (Orientation) : ?

DNA Sequence : Show Sequence

>gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
AGTATACCTTTGCAACC

Protein Sequence : Show Sequence

>gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
LLPESSLRKRKRSRC

Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
Additional Molecule Role : Vaximmutor
Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
Related Vaccine(s): C. pneumoniae CopN protein vaccine , C. pneumoniae LcrE protein vaccine

2. IgA

Gene Name : IgA
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 238447
Genbank Accession : AC160982
Taxonomy ID : 10090
Chromosome No : 12
Gene Starting Position : 8607
Gene Ending Position : 12639
Gene Strand (Orientation) : -
Protein Name : immunoglobulin heavy constant alpha
Protein Note : Also known as IgA; Igh-2

DNA Sequence : Show Sequence

>gi|121699722:8607-12639 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
AGTACTGGGGGACCTCTTTGCTGCCAAACGGGCCTCGAACAGTTGTAACAGTGAGTGCTGTGCTGTAGAA
CAAGCTCAGTAGGAAGAGGGTGAGGAAGGTCACAGTGGTGGGCCACAGGCTGGCACCTGGGGCCTCTTCC
TCCAGGATGTCTTCTGACTGGTCCAGTAGCACATAGGAAAGTGGCTCTTGACGTTCTGTAAGACATAGAT
GACCTTCTAGATCCAAACCCATGGGGATGGGGGCTCAGACTTAAGGTTTCTGCTTCTATGCCTCCTTGGG
CTTTGGGGACAGTGGTGGTAGGGGTCCCTTATTCCCGGGAAGGGACTTGTTCAGGGATAATGAGGAGGCA
GGGTCCCAGGTAAGGGACTGGAATCCCAGCAGTTCTTGAGATGGCCCCAAGACCCATCTCTCTCTCTTAG
GGAAACCTTAATTAGAGACCTGTCATGGTAAGAGAGTTGTTTCTGTCAAACTCTGAGAAGAGTTTGAACC
CAAGGCTCCTTAGGTCTCAGTCCTTTCTAGATATTTAGCAATACCTCCCTAAACCCCAGTTCTTAGGCTA
ATCTTGGCTTGGGTACTAGGGTTGTGTAAGATCAGACACCTTCAAACAGATACAGGCCAATGTGATTTCT
GGGCCAAAGTTAGACTACCAAGGCCTGGACATGTGGTCAGAGGACTCTGTTGCATTCTTAAGAGCTGTGA
CTGGGGGTTAAGCAACAGTAAAGACCCCCAGTAGAGCCAACCCTGCTGTCCTCAGGTTCTCTGCGCACAG
AGTTTCATTTTAAGTGGCAGTAGGAAATGTGTACTGATAGTCAACCACTCGTATTTCTGTCACTGCAGCC
ATCCATCAATCCATCCATCATCCCCATCGTTCATCCTTGTAGCCAGCCATCCATTACCCTCCAGCCGCTC
ACTCATCATCTTTCCCGACTATCGTGATTCTCCAGCTTCTCTGCCCCCTCTCCCTGTGGGGATTCAGTCT
TTCACCTATTGACTCCTCCTTACTAGGATTCTCTGTCATGGGCCAGGCCTTGGATTCCATGGGTGGCTAT
GGTACTTTTCTGAGTCAGGTTTCCCAAGCTGGTCTGTTGCTCTTGCTATTCTCAGGGAACTGGACTTTCA
AGCTGGAGAGTATCCAGGGCATGCATGGGCCTTTACTCCACTCTTGCTCTGAGAGCAGAGGGAAGTTGCT
AGAAACCAGCAACTCCTCCCCCCCCCCCCCGAAGTTCTGAAGGCCTGTGAGACAGGAGCAGGGCCCCCAC
TGCCCTCTTGTGGACACCTGGGCTCCAAGTATACCTGGTTGTAGAACTTTGTGTTCTAGGGGTGCCCATG
GAGGAGGCCGAGGCCTGTGTGGGAGTGGTCTCCATGGCCTGCTCCTGCTTCTTCCCAGATTTGCCTCTTA
TCCTGCTTCTGGTTTGTGATACTTGGGCTCAGGATGGGAAGGGAGACTTAGGTTAGAAAGGGGGCACCTG
GAGGGGCCTATGCCCTTGTCCATTGTGTGTAGTTGTCAGTGTCGGGCCTGGAAATTCTCCCGGGGGCTTG
GAGGGGACTCCCCTTGCTTTCCAGAAGCTGGGTGTGGGCTGTGAACTTCATCTTCCTCCAGGGTTCAGGT
CTGTGTGAGTGTGCAAATGTGAGTGTCTGTGCATGTATGCCCATGTGCATGTATGCAGAACAGGGTGTAT
GCCCATGTGCATGTGTGAAGAGCAGGGTGCTGCTGAGGGTTGTCTGTGCCATGCTATATGTCTTTCTCAT
GAGTCAGTGGTCACCCTGAATGCATGTTTCCATTCAGGGATGGCCTACTGAGGGTGCGTGGCATCTTCTT
CCCAGTGCCCCTGTGTTCATCCCTTCATTCTCATACCATCATCTCTATTGTGACCCCCACACGGCACCAG
CCTTGATGGTGCTACCCTTTTGCTCCATCTATCCCTGTCTTGTTTGGGTTAGAAGCTGCGGTCTAATAAA
CAGCCACACCACCCACCACTCAGAGTACTTGTGCAGCAACCCACTGCCCTGGGGACTCTCCCATGAGCTT
TGCCTTCTTGGCTGAGTCTGCTCGCCTTGGATTTCATCCCCAGTTTATTCTTACTTCCCATTCACTCATT
GCACATTCACAGGGCAGGTGCACACACTCTCATGTTTCCACCAACATGAACACACATAGATATATGCATG
CTCATAGGTATGCACAGGCACACACTTGCTCACAGGGACATAGATATAGTGTGTCCACATATGTACTTAT
ACCCATATACATACCTATAAACCATAGGTGCACACAAACTCACTCCTCTCTTGCTGCACACAGGTACACA
CATGAAGGCACATGGACCCATCCATAAGCACTCACCAATACATAGACATGCATACACATATACTCTAGGC
ATGAAGGGTCTTCAAAGTCTCCGAGGCCTTACAAGCTTAGGTGGTGGTGTTTCTCCCTCCCTGGGCCCTG
ACCCCTCCCTGTGTTCTGTAGACCCTAAGGATGATGGACAGGCACTGGATGGAAGTGCAGGGATACTTTG
GATGAGCACAGAGTTTATTTCAGGAGTAGGGACAGGCAGGGTGGCTCAGTAGCAGATGCCATCTCCCTCT
GACATGATCACAGACACGCTGACATTGGTGGGTTTACCCGACAGACGGTCGATGGTCTTCTGGGTGAAGT
TCATGGGCAAGGCCTCGTGGCCCACCATGCAGGAGTACTGGTCACCCTGTTTCCAGAGTTCAGCTGATAC
ACGCAACACGCTTGTCACCAGGTAGGTGGTGGCTCCCTCGCCTGGCTCCTTTAGGGGCTCAAACACTAGG
TAGCTTTCTGGGGACAGCTCCTCATTTCCATGCAGCCATCGCACCAGCACTTCTTTAGGGTTGAAAGCTC
GCACCAGGCATGTCAGGGACACGAGCTCATTCAGGGCCAGCTCCTCCGACGGCGGCGGTAGCAGGTGGAC
CTGGGGTGGGAAGGTGTTCACTGGAAGGTAGAAAGAAGAGTTATGGCTCAGACAAGGAGAGCAAGACCCC
TCTGCCCTTCCCTTCTGGGCAGTTCCTCAGATAGCTCCAATTTCCCTCTGTGATATAAGGACAGGAGCAG
TTAGTTGAGCATCTGTATTATAGGAAGAAAGCAGGCATGTGAGCAGGGAACGTCATACAATGTCCCGGGT
ATGCATCTGGGCTCACCTGTGATTTTGGCAATTGTGCCAGTTAAGGTGTCAGACTCAGGATGGGTAACTG
TGCACTTGAATGATGCGCCACTGTTCCAGCGCTCAGCACAGCCAGGCAGGACGCTGGACACACTGTAGCA
GCCGCAGGAATTCTGCACAGCTTTCTTCTGCACTGCATCCTTCCCAGTGGAGGGCTCCCAGGTGAAGACA
GCTCCCTCAGGATTTCTCAGGCCATTCAGAGTACATGTGAGGCTGGCATCTGAACCCAGGAGCAGGTCCT
CAAGAGCTGGCCGCTGCAGTGACAGGCTGGGATGGCAGGAAGGAGGACAAGGAGGACAAGGAGGAGGAGG
ACCTGTAACAGAGAAGTCCTAGCAAATCAGTATATTCTCCTTTTCTCCTTTGCTGTCCCCTTTTCTGGAT
GTTCTGAGGCCTCAGTTCCTTATGGCCTGTTTACGCTTCCCTTCCCCCACCCCCATGGGAGGTTTCTGAG
GGTCCCTGTGAGGGTGTGCCTCAGAGGGATATATGGATCTAGATAAGGTAGAACTTATCCCACCCCCAGC
TGACCCCCTAACGTTCTTTACCAGAGCACTTCACATCCAATTCTTGGACGGCGTTAGAGTCATGTTGCAC
GGAACATTTCACGGATTCTCCTTCTGGGCACTCGACAGCTGGCAGGGTCAACTGGCTGCTCATGGTGTAC
CCTCCCCCAGAGGCCAGGGCAGGTGGGAAGTTTACGGTGGTTATATCCTTCCCACTCTTTCCCCAGGTCA
CATTCATCGTGCCGGAAGGGAAGTAATCGTGAATCAGGCAGCCGATTATCACTGGGTCACTTGACAGAGC
TCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGAC

Molecule Role : Vaximmutor
Related Vaccine(s): C. pneumoniae LcrE protein vaccine

IV. Vaccine Information

1. C. pneumoniae CopN protein vaccine

a. Vaccine Ontology ID:

VO_0011432

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

C. pneumoniae copN

e. Gene Engineering of copN

Type: Recombinant protein preparation
Description: C. pneumoniae CopN (gene lcrE; position 0324 of C. pneumoniae CWL029), was produced in a Bacillus subtilis protein expression system as a soluble protein. Recombinant CopN protein was dissolved in PBS at a concentration of 1 mg/ml and heated to 100 °C for 10 min after which the visible precipitation of protein was discernible. C. pneumoniae preparation was boiled for 10 min in a water bath at a concentration of 2.5 × 10^7 IFU/ml in SPG. E. coli heat-labile toxin, LT (kindly provided by Prof. G. Dougan, Imperial Collage, London, UK) was added to heat-aggregated protein suspension to a final concentration of 12.5 μg/ml (Tammiruusu et al., 2007).
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0001322

g. Immunization Route

Intranasal

h. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: Mice were immunized intranasally with 40 μg of heat-aggregated CopN/ 40 μl dose or 106 heat-treated C. pneumoniae inclusion forming unit (IFU) (approximately 1 μg of protein)/40 μl dose. Mice immunized intranasally with disrupted HL cells (Mock) or PBS were used as control. Fourteen days after the first immunization, the mice were boosted once with the same dose of antigen. All immunizations were performed under methoxyflurane anaesthesia (Metofane, Pitman-Moore, Mundelein, IL, USA) (Tammiruusu et al., 2007).
Challenge Protocol: At 14 days after the second immunization, the mice were challenged intranasally with 10^5 IFU of C. pneumoniae in 40 μl of SPG under Metofane anaesthesia. At certain time points after infection, three to six mice were sacrificed, lungs were mechanically homogenized in SPG and dilutions of lung supernatant were cultured on HL cell monolayers (Tammiruusu et al., 2007).
Efficacy: Intranasal immunization of BALB/c mice with heat-aggregated CopN protein and an Escherichia coli heat-labile toxin (LT) induced a strong immune response. The immunization induced statistically significant protection against intranasal C. pneumoniae challenge, the level of which correlated with the magnitude of CopN-specific lymphocyte proliferation (Tammiruusu et al., 2007).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: The results of this study showed that intranasal immunization of BALB/c mice with heat-aggregated CopN protein and an Escherichia coli heat-labile toxin (LT) induced a strong immune response, detected as IFN-gamma production. The response was significant as compared to PBS-vaccinated mice in the lungs, spleen, and mediastinal lymph nodes 14 days after challenge (Tammiruusu et al., 2007).
- Detailed Gene Information: Click here.

2. C. pneumoniae DNA vaccine encoding FabD

a. Vaccine Ontology ID:

VO_0011434

b. Type:

DNA vaccine

c. Status:

Research

d. Antigen

C. pneumoniae fabD

e. Gene Engineering of FabD

Type: DNA vaccine construction
Description: The genome sequence of C. pneumoniae isolate CDC/CWL-029 (ATCC strain VR-1310) was extracted from Genbank (AE001363, 1,230,230 bp). The 1052 annotated genes of C. pneumoniae were imported into a gene-splitting and primer prediction program; primer pairs to amplify 1263 ORFs of 1.5 kb or less were exported. A 1.5 kb maximum ORF length was chosen to ensure sufficient PCR quality and yields, and this generated a few additional fragments (Li et al., 2006).
Detailed Gene Information: Click here.

f. Vector:

pCMVi-UB or linear expression elements (Li et al., 2006)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Host Strain: A/J
Vaccination Protocol: For intranasal inoculation, mice received a light isoflurane inhalation anesthesia. Vaccine protection control mice were inoculated with a low dose of 5 × 10^6 C. pneumoniae elementary bodies in 30 μl SPG buffer (Li et al., 2006).
Challenge Protocol: High-dose challenge infection was performed 4 weeks after the last gene gun genetic vaccination or low dose inoculation of live C. pneumoniae, and 6 weeks after the last intramuscular-intradermal genetic vaccination, by intranasal inoculation of 1 × 10^8 C. pneumoniae elementary bodies in 30 μl SPG buffer. Mice were sacrificed by CO2 inhalation 2 h, 3 days, 10 days, or 15 days after inoculation, and lungs and spleen were weighed, snap frozen in liquid nitrogen, and stored at −80 °C until further processing (Li et al., 2006).
Efficacy: M-ID vaccination with fabD generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of fabD to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).

3. C. pneumoniae DNA vaccine encoding PknD

a. Vaccine Ontology ID:

VO_0011424

b. Type:

DNA vaccine

c. Status:

Research

d. Antigen

C. pneumoniae serine/threonine-protein kinase, PknD

e. Gene Engineering of PknD

Type: DNA vaccine construction
Description: The genome sequence of C. pneumoniae isolate CDC/CWL-029 (ATCC strain VR-1310) was extracted from Genbank (AE001363, 1,230,230 bp). The 1052 annotated genes of C. pneumoniae were imported into a gene-splitting and primer prediction program; primer pairs to amplify 1263 ORFs of 1.5 kb or less were exported. A 1.5 kb maximum ORF length was chosen to ensure sufficient PCR quality and yields, and this generated a few additional fragments (Li et al., 2006).
Detailed Gene Information: Click here.

f. Vector:

pCMVi-UB or linear expression elements (Li et al., 2006)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Host Strain: A/J
Vaccination Protocol: For intranasal inoculation, mice received a light isoflurane inhalation anesthesia. Vaccine protection control mice were inoculated with a low dose of 5 × 10^6 C. pneumoniae elementary bodies in 30 μl SPG buffer (Li et al., 2006).
Challenge Protocol: High-dose challenge infection was performed 4 weeks after the last gene gun genetic vaccination or low dose inoculation of live C. pneumoniae, and 6 weeks after the last intramuscular-intradermal genetic vaccination, by intranasal inoculation of 1 × 10^8 C. pneumoniae elementary bodies in 30 μl SPG buffer. Mice were sacrificed by CO2 inhalation 2 h, 3 days, 10 days, or 15 days after inoculation, and lungs and spleen were weighed, snap frozen in liquid nitrogen, and stored at −80 °C until further processing (Li et al., 2006).
Efficacy: IM-ID vaccination with CPn0095 (pknD) generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of CPN0095 to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).

4. C. pneumoniae DNA vaccine encoding Ssb

a. Vaccine Ontology ID:

VO_0011425

b. Type:

DNA vaccine

c. Status:

Research

d. Antigen

C. pneumoniae single-stranded DNA-binding protein, ssb

e. Gene Engineering of Ssb

Type: DNA vaccine construction
Description: The genome sequence of C. pneumoniae isolate CDC/CWL-029 (ATCC strain VR-1310) was extracted from Genbank (AE001363, 1,230,230 bp). The 1052 annotated genes of C. pneumoniae were imported into a gene-splitting and primer prediction program; primer pairs to amplify 1263 ORFs of 1.5 kb or less were exported. A 1.5 kb maximum ORF length was chosen to ensure sufficient PCR quality and yields, and this generated a few additional fragments (Li et al., 2006).
Detailed Gene Information: Click here.

f. Vector:

pCMVi-UB or linear expression elements (Li et al., 2006)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Host Strain: A/J
Vaccination Protocol: For intranasal inoculation, mice received a light isoflurane inhalation anesthesia. Vaccine protection control mice were inoculated with a low dose of 5 × 10^6 C. pneumoniae elementary bodies in 30 μl SPG buffer (Li et al., 2006).
Challenge Protocol: High-dose challenge infection was performed 4 weeks after the last gene gun genetic vaccination or low dose inoculation of live C. pneumoniae, and 6 weeks after the last intramuscular-intradermal genetic vaccination, by intranasal inoculation of 1 × 10^8 C. pneumoniae elementary bodies in 30 μl SPG buffer. Mice were sacrificed by CO2 inhalation 2 h, 3 days, 10 days, or 15 days after inoculation, and lungs and spleen were weighed, snap frozen in liquid nitrogen, and stored at −80 °C until further processing (Li et al., 2006).
Efficacy: Mice vaccinated with candidate gene ssb showed significant reduction of spleen chlamydial loads as compared to naïve, non-protected control mice (p ≤ 0.048). This resulted in the ability of ssb to mediate a modest, but significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).

5. C. pneumoniae DNA vaccine pHSP-60

a. Vaccine Ontology ID:

VO_0004555

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of GroEL

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

f. Vector:

pCI (Svanholm et al., 2000)

g. Immunization Route

intranasal immunization

h. Mouse Response

Vaccine Immune Response Type: VO_0003057
Efficacy: Immunization with this vaccine resulted in signi®cant protection, as measured by a lower bacterial load and a less severe pathological outcome after infection with C. pneumoniae (Svanholm et al., 2000).

6. C. pneumoniae LcrE protein vaccine

a. Vaccine Ontology ID:

VO_0011435

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

C. pneumoniae LcrE

e. Gene Engineering of LcrE

Type: Recombinant protein preparation
Description: A 1218-kb DNA fragment containing the lcrE gene (GenBank ID 15618244, Locus tag CPn0324) was amplified by PCR, using C. pneumoniae (CWL029 ATCC) DNA as template. The PCR was performed in a GeneAmp II (Applied Biosystems, Foster City, CA, USA) thermocycler with Advantage GC cDNA polymerase (Clontech, Mountain View, CA, USA), and the amplification conditions were set as recommended by the manufacturer. The amplicon was digested with NdeI and BamHI and inserted into p6HisF-11d (icl) pET vector by digesting it with the same enzymes and replacing the icl gene (Faludi et al., 2009).
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0000884
Description: Either Freund's or Alum adjuvants

g. Adjuvant:

VO ID: VO_0000133

h. Immunization Route

Subcutaneous injection

i. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: The mice in groups of 25 were immunized subcutaneously into the tail base with the purified LcrE protein diluted in phosphate buffered saline (PBS) at a dose of 20 μg mixed with 25 μl Alum (Aluminum hydroxide Gel, Sigma) or 75 μl Freund's adjuvants (Chemicon International, Temecula, CA, USA; 1st inoculation with complete and 2nd and 3rd inoculations with incomplete Freund's adjuvant) in 0.15-ml volume 3 times at 3-week intervals (Faludi et al., 2009).
Challenge Protocol: Two weeks after the last immunization, the immunized and non-immunized mice (absolute naive animals) were challenged with 4×10^5 inclusion forming unit (IFU) C. pneumoniae (CWL029, ATCC) in 25 μl PBS intranasally under pentobarbital sodium anesthesia (Faludi et al., 2009).
Efficacy: The immunogenicity and protective effect of recombinant LcrE protein combined either with Freund's or Alum adjuvant were investigated in mice. The immunization with both protocols resulted in a significant reduction of the number of viable C. pneumoniae in the lungs after challenge. Results confirm that LcrE induces protective immunity in mice (Faludi et al., 2009).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: The presence of LcrE-specific IFN-gamma-producing cells in LcrE+Alum-immunized mice indicates Th1 type response. IFN-gamma responses were measured in spleen cells collected 2 weeks after last immunization and were significantly higher than mock-immunized mice (Faludi et al., 2009).
- Detailed Gene Information: Click here.
Host Gene Response of IgA
- Gene Response: LcrE-specific IgA level was higher in both the sera and the lungs after using Freund's adjuvant than non-immunized mice at the time of the challenge (Faludi et al., 2009).
- Detailed Gene Information: Click here.

V. References

1. Atanu et al., 2013: Atanu FO, Oviedo-Orta E, Watson KA. A novel transport mechanism for MOMP in Chlamydophila pneumoniae and its putative role in immune-therapy. PloS one. 2013; 8(4); e61139. [PubMed: 23637791].

2. Faludi and Szab�, 2011: Faludi I, Szab� �M. Vaccination with DNA vector expressing chlamydial low calcium response protein E (LcrE) against Chlamydophila pneumoniae infection. Acta microbiologica et immunologica Hungarica. 2011; 58(2); 123-134. [PubMed: 21715282].

3. Faludi et al., 2009: Faludi I, Burian K, Csanadi A, Miczak A, Lu X, Kakkar VV, Gonczol E, Endresz V. Adjuvant modulation of the immune response of mice against the LcrE protein of Chlamydophila pneumoniae. International journal of medical microbiology : IJMM. 2009; 299(7); 520-528. [PubMed: 19451031].

4. Finco et al., 2005: Finco O, Bonci A, Agnusdei M, Scarselli M, Petracca R, Norais N, Ferrari G, Garaguso I, Donati M, Sambri V, Cevenini R, Ratti G, Grandi G. Identification of new potential vaccine candidates against Chlamydia pneumoniae by multiple screenings. Vaccine. 2005; 23(9); 1178-1188. [PubMed: 15629361].

5. Li et al., 2006: Li D, Borovkov A, Vaglenov A, Wang C, Kim T, Gao D, Sykes KF, Kaltenboeck B. Mouse model of respiratory Chlamydia pneumoniae infection for a genomic screen of subunit vaccine candidates. Vaccine. 2006; 24(15); 2917-2927. [PubMed: 16434129].

6. Lu et al., 2012: Lu X, Xia M, Endresz V, Faludi I, Szabo A, Gonczol E, Mundkur L, Chen D, Kakkar V. Impact of multiple antigenic epitopes from ApoB100, hHSP60 and Chlamydophila pneumoniae on atherosclerotic lesion development in Apob(tm2Sgy)Ldlr(tm1Her)J mice. Atherosclerosis. 2012; 225(1); 56-68. [PubMed: 22959702].

7. Penttilä et al., 2000: Penttilä T, Vuola JM, Puurula V, Anttila M, Sarvas M, Rautonen N, Mäkelä PH, Puolakkainen M. Immunity to Chlamydia pneumoniae induced by vaccination with DNA vectors expressing a cytoplasmic protein (Hsp60) or outer membrane proteins (MOMP and Omp2). Vaccine. 2000; 19(9-10); 1256-1265. [PubMed: 11137265].

8. Svanholm et al., 2000: Svanholm C, Bandholtz L, CastaÃ±os-Velez E, Wigzell H, Rottenberg ME. Protective DNA immunization against Chlamydia pneumoniae. Scandinavian journal of immunology. 2000; 51(4); 345-353. [PubMed: 10736106].

9. Tammiruusu et al., 2007: Tammiruusu A, Penttilä T, Lahesmaa R, Sarvas M, Puolakkainen M, Vuola JM. Intranasal administration of chlamydial outer protein N (CopN) induces protection against pulmonary Chlamydia pneumoniae infection in a mouse model. Vaccine. 2007; 25(2); 283-290. [PubMed: 16949182].

10. Tammiruusu et al., 2007: Tammiruusu A, Penttil� T, Lahesmaa R, Sarvas M, Puolakkainen M, Vuola JM. Intranasal administration of chlamydial outer protein N (CopN) induces protection against pulmonary Chlamydia pneumoniae infection in a mouse model. Vaccine. 2007; 25(2); 283-290. [PubMed: 16949182].

11. Wiki: Chlamydophila pneumoniae: Chlamydophila pneumoniae [http://en.wikipedia.org/wiki/Chlamydophila_pneumoniae]