VIOLIN: Vaccine Investigation and Online Information Network

MIC8
Gene Name	MIC8
NCBI Gene ID	7899500
NCBI Nucleotide GI	13346486
NCBI Protein GI	13346487
Protein Accession	AAK19757.1
Taxonomy ID	5811
Gene Strand (Orientation)	?
Protein Name	microneme protein 8
Protein pI	5.13
Protein Weight	71329.71
Protein Length	747
DNA Sequence	>gi\|13346486\|gb\|AF353165.1\| Toxoplasma gondii microneme protein 8 (MIC8) gene, complete cds ATGAAGGCCAATCGAATATGGTGTTTTTTTGCGTGGCGTATGGTTGTGCGGGCCTCATTTCTGAAAGAGA TGGACAGCATTTTCGTTTCTGCTATCCGACAGAATGTACAGCATACTCATTCTGCCCTTCTCGCCAAACT GAAGGAACCCCCAGATCCAGATGATGAGAACTCTTGGCTTTGTCGAATATCAAAAAAATATGACGCATGC GGTAGCAGGGAATATTCCGATAAGGGCCTCAAAGGGACGTACTGTCCCGAGGATTTTTGCTGTAGCAAGA CGGCATGTTTTTACGGTTCATGTGGGAGTTGGTGCCACGACAACTGGGCTCTGTGCAGCTCATCTATAAT CTACCACGACGAGTACAGTTACGGGAAATGCAACTGTAAACGGTTTCAAGAAAACTGTGATGTGAATGCA ATTTGTGTGCATGCGAACAGAGAGGATGGCGGTGCGTATTGTCAGTGCAAGGAAGGATATTGGGGTGATG GTAAATCGTGCAAGATTGACTTCTGCCAACTGCAGCCCTGTGGTGCAGGGACCTGCACCAGGACGGATGA AGGATACAAGTGTGATTGCCCAGAAACTCACAAGCTTATTGTCGTTGAAGACAAAGAGACGTGCAAGGCA AAACCGGACTTTTGCGCGGAAGAGCCTTGCGGACCACCCTCTATGGTTGAAAATTGCGTGAACACCGATG ACAGCTACGAATGTGTTTGCAAACAGGGGTATGAAGTGAGGAACGGTCGGTGCGAAGAAATTGACTTATG CGCGGACAAGCCATGTGGGCCAGATGAGGGTGTGCATGAGTGTGTAACAGAGAGGCAACCGAAATTAAGG TACAGATGCACGTGCAAGGCAGGATTCGATTTGACCACCTTGCCTGATGGTGTTTCCCAGAAGTGCCTGA AGAACTTCTGTTACGAGGAGCCCTGCGGCACCCGGGACCTAGTTGAAAGCTGTAAGTCAAAGGCATATGG ATACTCGTGTTTGTGTGCGGCAGGTGCCATGGTTCAAGTGATTAACGGAAAAGAAAAGTGCATCAAGGCG GACTTGTGCCGCAATGATCCGTGTGGTCCAGAGACAGCAGTGATTCAATGTTACTCTCATGGCACCAGCT ATAGGTGTTTGTGCAAAGCAGGCTACACTGAAGTTTTTGTTAACGGGAAGAGTTCATGTCAAAAGGGCGA CCCATGCACTCTGAACATGTGTGGCGGTAACGAAGCGGTCCAGGAGTGCACAACTGATGGCACGGCGTAC GGGTGTACCTGCAAGCCAGGCTATTCGATAGCCATTAAGCATGGTCAGAAGTTTTGCAACCCTGAAGAGG AGTGTGCTTCTCATTGTGGCTCGGCAGCTGCAGTGAAAAGCTGTGAAATACTTGACTCTGGCGGATACCA GTGTACATGCAATCCAGGATACGTCATGAGATACAGCGACTATGTAAAAGGATGCGTCGAGGGAAATCAG TGTTCTCTCAATCCTTGTGGGGAGCAGGAAGCCGTGCAAAGGTGCATTCCTGAAGGTGACACGTATGATT GCGAGTGCAATCCGGGGTTCGTCAAAAGAGTCTTGCCGGATGGGAATTTCATTTGCGCCGATCCAGCGAG CTGTGTAGGGAATCCCTGTGGTAGCTCAGATGCGGTCGATGCGTGCATTGCCGGGACTAGCACGTATACA TGCAGGTGTAAGGACGGATACACACCTCAGTCAATTGGGTCAAAGTTGCAGTGTTTACCAGAAAGCACTG ATCAGACAGATTTCGATTCCAAACACAAACCAGAGGACAACAAAGGTCGATATTCGAAAGGAACAATTGC ATTGGTGGTTGTGGGGTGTGTAGCCTTGTTGGGTATTATAGCCGGAGGAATTTCTTACGCCAGAAACAGA GGAGGTGAGCGCGATGATGAAGACTTGGCACCACCACCTCGTTCCACACGAGAACGGAGGCTCTCATCAA TGGGCGAAGGTTTTGAGAATGCCTCATGGGCATCTTCTGTAAGTATGATTCCTAGTGCACCTGCTCCGCC ACCTTCGGGCGGTATCTGGTCCTAA
Protein Sequence	>AAK19757.1 microneme protein 8 [Toxoplasma gondii] MKANRIWCFFAWRMVVRASFLKEMDSIFVSAIRQNVQHTHSALLAKLKEPPDPDDENSWLCRISKKYDAC GSREYSDKGLKGTYCPEDFCCSKTACFYGSCGSWCHDNWALCSSSIIYHDEYSYGKCNCKRFQENCDVNA ICVHANREDGGAYCQCKEGYWGDGKSCKIDFCQLQPCGAGTCTRTDEGYKCDCPETHKLIVVEDKETCKA KPDFCAEEPCGPPSMVENCVNTDDSYECVCKQGYEVRNGRCEEIDLCADKPCGPDEGVHECVTERQPKLR YRCTCKAGFDLTTLPDGVSQKCLKNFCYEEPCGTRDLVESCKSKAYGYSCLCAAGAMVQVINGKEKCIKA DLCRNDPCGPETAVIQCYSHGTSYRCLCKAGYTEVFVNGKSSCQKGDPCTLNMCGGNEAVQECTTDGTAY GCTCKPGYSIAIKHGQKFCNPEEECASHCGSAAAVKSCEILDSGGYQCTCNPGYVMRYSDYVKGCVEGNQ CSLNPCGEQEAVQRCIPEGDTYDCECNPGFVKRVLPDGNFICADPASCVGNPCGSSDAVDACIAGTSTYT CRCKDGYTPQSIGSKLQCLPESTDQTDFDSKHKPEDNKGRYSKGTIALVVVGCVALLGIIAGGISYARNR GGERDDEDLAPPPRSTRERRLSSMGEGFENASWASSVSMIPSAPAPPPSGGIWS
Molecule Role	Protective antigen
Molecule Role Annotation	The gene sequence encoding MIC8 was inserted into the eukaryotic expression vector pVAX I, and the pVAX-MIC8 expression plasmid was constructed, and the plasmid diluted with PBS to 100 mg/100 microl was injected into the Kunming mice muscularly. Levels of IgG antibody, gamma-interferon (IFN-gamma), interleukin-2 (IL-2), interleukin-4, and interleukin-10 were detected. The mice were challenged with tachyzoites of the virulent T. gondii RH strain at the 14th day after the last immunization to observe the survival time. The high level of IFN-gamma, IL-2, and IgG antibody indicated that mice vaccinated with recombinant pVAX-MIC8 plasmid could elicit strong cellular and humoral immune responses and showed a significantly increased survival time (10.3 +/- 0.9 days) compared with control mice which died within 5 days of challenge infection (Liu et al., 2010).
Related Vaccines(s)	T. gondii DNA vaccine encoding MIC8
References

MIC8