VIOLIN: Vaccine Investigation and Online Information Network

nucA
Gene Name	nucA
Sequence Strain (Species/Organism)	Haemophilus influenzae strain P860295
VO ID	VO_0012405
NCBI Protein GI	4929317
Other Database IDs	CDD:211667
Taxonomy ID	727
Gene Strand (Orientation)	?
Protein Name	5'-nucleotidase NucA precursor
Protein pI	7.13
Protein Weight	62950.55
Protein Length	681
Protein Note	NAD pyrophosphatase/5'-nucleotidase NadN; TIGR01530
Protein Sequence	>AAD33949.1 5'-nucleotidase NucA precursor [Haemophilus influenzae] MLLSKKSATFALSVFAMLFTSVALAKEAPQAHKAVELSILHINDHHSYLEPHETRINLNGQQTKVDIGGF SAVNAKLNKLRKKYKNPLVLHAGDAITGTLYFTLFGGSADAAVMNAGNFHYFTLGNHEFDAGNEGLLKLL EPLKIPVLSANVIPDKSSILYNKWKPYDIFTVDGEKIAIIGLDTVNKTVNSSSPGKDVKFYDEIATAQIM ANALKQQGINKIILLSHAGSEKNIEIAQKVNDIDVIVTGDSHYLYGNDELRSLKLPVIYEYPLEFKNPNG EPVFVMEGWAYSAVVGDLGVKFSPEGIASITRKIPHVLMSSHKLQVKNSEGKWAELTGDERKKALDTLKS MKSISLDDHDAKTDKLIAKYKSEKDRLAQEIVGVITGSAMPGGSANRIPNKAGSNPEGSIATRFIAETMY NELKTVDLTIQNAGGVRADILPGNVTFNDAYTFLPFGNTLYTYKMEGSLVKQVLEDAMQFALVDGSTGAF PYGAGIRYEANETPNAEGKRLVSVEVLNKQTQQWEPIDDNKRYLVGTNAYVAGGKDGYKTFGKLFNDPKY EGVDTYLPDAESFIKFMKKHPHFEAYTSSNVKFNASTDALPKK
Molecule Role	Protective antigen
Molecule Role Annotation	Mouse antiserum directed against the recombinant NucA protein was reactive on Western immunoblots and whole-cell ELISA with all H. influenzae strains tested including Eagan and was bactericidal for two heterologous strains tested. The antiserum also resulted in a log reduction in bacteremia, in an infant-rat protection study with H. influenzae type b as the challenge strain (Zagursky et al., 2000).
Related Vaccines(s)	Nontypeable H. influenzae NucA Protein vaccine
References

nucA