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Cruzipain
Gene Name	Cruzipain
Sequence Strain (Species/Organism)	Trypanosoma cruzi
VO ID	VO_0011293
NCBI Gene ID	3550848
NCBI Protein GI	71663165
Genbank Accession	AAHK01000135
Protein Accession	XP_818579
Other Database IDs	CDD:185513 CDD:214853 CDD:278538 CDD:239068 CDD:288946
Taxonomy ID	5693
Chromosome No	Unknown
Gene Starting Position	102406
Gene Ending Position	103809
Gene Strand (Orientation)	+
Protein Name	cruzipain
Protein pI	6.01
Protein Weight	45629.84
Protein Length	467
Protein Note	internal copy from a tandem array
DNA Sequence	>NW_001849391.1:102406-103809 Trypanosoma cruzi strain CL Brener 1047053516253 genomic scaffold, whole genome shotgun sequence AATGTCTGGCTGGGCGCGTGCGCTGTTGCTCGCGGCCGTCCTGGTCGTCATGGCGTGCCTTGTCCCCGCG GCGACGGCGAGCCTGCATGCGGAGGAGACGCTGACGTCGCAATTCGCAGAATTCAAGCAGAAGCATGGCA GGGTGTACGAGAGCGCCGCGGAGGAGGCGTTCCGCCTGAGCGTGTTCAGGGAGAACCTGTTTCTTGCGAG GCTGCACGCCGCGGCAAACCCACACGCGACCTTCGGCGTCACGCCCTTCTCGGACCTCACGCGCGAGGAA TTCCGGTCCCGCTACCACAACGGCGCGGCGCACTTTGCGGCGGCGCAGGAGCGCGCGAGAGTGCCGGTGA AGGTGGAGGTAGTTGGCGCGCCCGCGGCAGTGGATTGGCGTGCGAGAGGCGCCGTGACAGCCGTCAAGGA CCAGGGCCAATGCGGTTCGTGCTGGGCCTTCTCCGCCATTGGTAACGTTGAGTGCCAGTGGTTTCTTGCC GGCCACCCGCTGACGAACCTGTCGGAGCAGATGCTCGTGTCGTGCGACAAAACGGACTTTGGCTGCAGTG GTGGCCTGATGAACAACGCCTTTGAGTGGATTGTGCAGGAGAATAACGGCGCCGTGTACACGGAGGACAG CTACCCTTATGCGTCGGGCGAGGGGATATCGCCGCCGTGCACGACGTCAGGCCACACGGTGGGTGCCACG ATTACCGGTCACGTTGAATTACCTCAGGACGAGGCCCAAATAGCCGCATGGCTTGCAGTCAATGGCCCGG TTGCCGTTGCCGTCGACGCCAGCAGCTGGATGACCTACACGGGCGGCGTTATGACGAGCTGCGTCTCCGA GCAGCTGGATCACGGCGTTCTTCTCGTCGGCTACAATGACAGCGCCGCAGTGCCGTACTGGATCATCAAG AACTCGTGGACCACGCAGTGGGGCGAGGAAGGCTACATCCGCATTGCAAAGGGCTCGAACCAGTGCCTTG TCAAGGAGGAGGCGAGCTCCGCGGTGGTCGGTGGTCCCGGACCCACTCCCGAGCCAACCACCACGACAAC CACAAGTGCCCCAGGACCGTCCCCATCGTACTTTGTGCAGATGTCCTGCACTGACGCTGCGTGCATTGTC GGGTGCGAGAACGTGACGTTACCGACCGGTCAGTGTCTCCTGACCACCAGCGGCGTCTCTGCCATTGTCA CGTGCGGTGCTGAGACTCTCACAGAAGAAGTCTTCCTTACGAGTACGCACTGCAGCGGCCCATCGGTGAG GTCCTCTGTTCCTCTCAACAAATGCAACCGGCTTTTAAGAGGCTCCGTTGAGTTCTTCTGCGGCTCCAGC TCCAGTGGCCGACTGGCCGACGTGGACAGGCAGCGTCGCCATCAGCCATACCACAGCCGTCATCGCCGCC TCTG
Protein Sequence	>XP_818579.1 cruzipain precursor, putative [Trypanosoma cruzi] MSGWARALLLAAVLVVMACLVPAATASLHAEETLTSQFAEFKQKHGRVYESAAEEAFRLSVFRENLFLAR LHAAANPHATFGVTPFSDLTREEFRSRYHNGAAHFAAAQERARVPVKVEVVGAPAAVDWRARGAVTAVKD QGQCGSCWAFSAIGNVECQWFLAGHPLTNLSEQMLVSCDKTDFGCSGGLMNNAFEWIVQENNGAVYTEDS YPYASGEGISPPCTTSGHTVGATITGHVELPQDEAQIAAWLAVNGPVAVAVDASSWMTYTGGVMTSCVSE QLDHGVLLVGYNDSAAVPYWIIKNSWTTQWGEEGYIRIAKGSNQCLVKEEASSAVVGGPGPTPEPTTTTT TSAPGPSPSYFVQMSCTDAACIVGCENVTLPTGQCLLTTSGVSAIVTCGAETLTEEVFLTSTHCSGPSVR SSVPLNKCNRLLRGSVEFFCGSSSSGRLADVDRQRRHQPYHSRHRRL
Molecule Role	Protective antigen
Molecule Role Annotation	To study the protective effects in vivo of cruzipain-specific Th1 responses against systemic T. cruzi challenges, mice were immunized with recombinant cruzipain plus interleukin 12 (IL-12) and a neutralizing anti-IL-4 MAb. These immunized mice developed potent cruzipain-specific memory Th1 cell responses and were significantly protected against normally lethal systemic T. cruzi challenges. To study whether cruzipain could induce mucosal immune responses relevant for vaccine development, recombinant attenuated Salmonella enterica serovar Typhimurium vaccines expressing cruzipain were prepared. BALB/c mice immunized with salmonella expressing cruzipain were significantly protected against T. cruzi mucosal infection (Schnapp et al., 2002).
Related Vaccines(s)	T. cruzi vaccine using attenuated Salmonella expressing T. cruzi Cruzipain
References

Cruzipain