VIOLIN: Vaccine Investigation and Online Information Network

M2
Gene Name	M2
Sequence Strain (Species/Organism)	Leishmania amazonensis
VO ID	VO_0011291
NCBI Nucleotide GI	2828562
NCBI Protein GI	2828563
Protein Accession	AAC08302.1
Other Database IDs	CDD:240315 CDD:153108
Taxonomy ID	5659
Gene Strand (Orientation)	?
Protein Name	ribonucleotide reductase M2 subunit
Protein pI	5.07
Protein Weight	38155.95
Protein Length	424
Protein Note	hydroxyurea-resistant strain derived from LV-78; cloned from extrachromosomal amplified circular DNA
DNA Sequence	>gi\|2828562\|gb\|AF005247.1\| Leishmania mexicana amazonensis extrachromosomal element ribonucleotide reductase M2 subunit gene, complete cds ATGAAACCGGTTGCGGCTGGCGCCGAGGTGCTGCCGGCGGACAAGGTTGCCCAGGGGACGAACGCCGAGG AGGAGCCGCTGCAGCAGGAGAACCCGTTCCGCTACGTCCTCTTCCCAATCCAGTACCATGACATCTGGCG GAAGTACAAGGAGCAGGAGAGCTGCATCTGGACGGTGGAGGAGATCGACCTGGGCAACGACATGAAGGAC TGGGCAACGCTGAACGACGGTGAGCGGCACTTCATCAAGCATGTGCTTGCCTTCTTCGCCGGCAGCGACG GCATAGTCATCGAGAATCTTGCGCAGCGCTTCATGAGCGACGTGAAGGTGCCAGAGGCGCGCGCGTTCTA CGGGTTCCAGCTGATGATGGAGAACATCCACTCGGAGACGTACTCTGTGCTGCTGGACACGTACATCACG GACAGCGAGGAGAAGCTGCGGCTGCTGCACGCGATACAGACGATCCCGTGCATCCAGAAGAAGGCGGAGT GGCCCGTGCGGTGGATCGGGAGCAGCGCGAGCTTTCAGCAGCGGCTGATCGGCTTTGCCGCGGTGGAGGG CATTTTCTTTTCCGGGTCGTTCTGCGCGCTGTTCTGGCTGAAGAAGCGCGGTCTGATGCCAGGGCTGACG TTCAGCAACGAGCTCATCTCGCGCGACGAGGGTCTACACACGGACTTCGCATGCCTGCTGTACAACTCGC ACATCAAGCACAAGCTGCCGCGCGAGCGCGTGCTGGAGATCATCGTGGATGCGGTGAACATCGAGCGTGA GTTCATCTGCGACGCGCTGCCGGTGCGGCTGATTGGCATGAACGCGGAGCTGATGGCGCAGTACATCGAG TTCGTCGCGGACCGGCTGCTGGTGTCGCTCGGCGAGGAGAAGCACTACCGCTCGACGCAGCCGTTCGACT TCATGGAGATGATCTCGCTGCAGGGCAAAACGAACTTCTTCGAGAAGAGGGTGGGGGAGTACCAGAAGGC CGGCGTGATGAGCACGGAGGGCACGTCGAAAAAGTTCTCCCTCTCGGAGGACTTCTAA
Protein Sequence	>AAC08302.1 ribonucleotide reductase M2 subunit [Leishmania amazonensis] MKPVAAGAEVLPADKVAQGTNAEEEPLQQENPFRYVLFPIQYHDIWRKYKEQESCIWTVEEIDLGNDMKD WATLNDGERHFIKHVLAFFAGSDGIVIENLAQRFMSDVKVPEARAFYGFQLMMENIHSETYSVLLDTYIT DSEEKLRLLHAIQTIPCIQKKAEWPVRWIGSSASFQQRLIGFAAVEGIFFSGSFCALFWLKKRGLMPGLT FSNELISRDEGLHTDFACLLYNSHIKHKLPRERVLEIIVDAVNIEREFICDALPVRLIGMNAELMAQYIE FVADRLLVSLGEEKHYRSTQPFDFMEMISLQGKTNFFEKRVGEYQKAGVMSTEGTSKKFSLSEDF
Molecule Role	Protective antigen
Molecule Role Annotation	Immunization of CBA mice with the M-2 glycoprotein of L. amazonensis and C. parvum adjuvant resulted in complete protection against a challenge infection of 10^4 and 10^6 late log-phase promastigotes of L. amazonensis. In the BALB/c strain, complete protection was observed in some of the immunized animals (28 to 50%); in the rest of the mice the onset of infection was significantly delayed. Protective immunity for C57BL/6 mice was observed only at the low infecting dose (10(4) L. amazonensis organisms) (Champsi and McMahon-Pratt, 1988).
Related Vaccines(s)	L. amazonensis M2 protein vaccine
References

M2