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Sfs
Gene Name	Sfs
Sequence Strain (Species/Organism)	Streptococcus equi subsp. equi 4047
VO ID	VO_0011215
NCBI Gene ID	7696331
NCBI Protein GI	225869898
Locus Tag	SEQ_0466
Genbank Accession	FM204883
Protein Accession	YP_002745845
Taxonomy ID	553482
Gene Starting Position	452509
Gene Ending Position	453624
Gene Strand (Orientation)	?
Protein Name	fibronectin-binding protein Sfs
Protein pI	8.97
Protein Weight	39068.51
Protein Length	371
Protein Note	Previously sequenced as Streptococcus equi fibronectin-binding protein Sfs UniProt: AF136451 (371 aa" target="_blank">Q9X6F4 (EMBL: AF136451 (371 aa) fasta scores: E()=1.8e-153, 100.000% id in 371 aa
DNA Sequence	>NC_012471.1:452509-453624 Streptococcus equi subsp. equi 4047, complete genome TATGAGAAAAACAGAAGGACGTTTTCGCACATGGAAGTCCAAAAAACAATGGCTATTTGCCGGTGCAGTG GTGACCAGCTTGCTGCTGGGAGCTGCACTTGTCTTTGGAGGTTTATTAGGAAGTCTTGGTGGCTCATCCC ATCAAGCGAGGCCAAAGGAGCAGCCAGTCAGCTCGATTGGAGATGACGATAAGTCGCACAAGAGCTCATC AGATTCAATGGTCAGTCGGCCACCGAAAAAGGATAACTTGCAGCCTAAGCCTTCAGATCAGCCTACTAAT CATCAGCATCAGGCTACTAGCCCGTCCCAGCCGACAGCAAAGAGCTCAGGTCATCATGGGAATCAACCTC AGTCTCTCAGTGTGAACAGCCAAGGAAATAGTAGTGGACAGGCCTCAGAGCCTCAGGCTATTCCTAATCA AGGGCCTTCCCAGCCACTGGGGCTGAGAGGAGGTAACAGCTCTGGTTCAGGTCATCACCATCAGCCACAA GGAAAGCCACAGCACTTAGATCTAGGTAAGGATAATTCTAGCCCGCAGCCTCAACCAAAGCCTCAGGGCA ATAGTCCCAAGCTGCCAGAAAAAGGCTTGAATGGTGAAAATCAGAAGGAACCGGAGCAAGGTGAACGAGG TGAGGCTGGTCCCCCACTTTCAGGGTTGAGTGGTAATAATCAAGGCCGTCCTTCGCTTCCAGGCTTGAAT GGTGAGAATCAGAAGGAACCAGAGCAAGGTGAACGAGGTGAAGCCGGTCCCCCATCAACTCCGAATTTAG AAGGAAATAATCGTAAAAATCCTTTAAAAGGATTAGATGGAGAGAATAAGCCAAAGGAAGATTTAGACGG TAAAGGCTTAAGCGGAGAAAATGATGAATCACCAAAACTTAAAGACGAACACCCCTACAATCATGGTCGT CGTGATGGTTACCGAGTTGGCTATGAGGATGGATATGGTGGCAAAAAGCACAAAGGAGATTATCCTAAGC GGTTTGATGAGTCTAGTCCAAAGGAATATAATGACTATAGTCAAGGGTATAATGATAATTATGGAAATGG CTATTTAGATGGTCTAGCTGATAGAGGTGGTAAGAGAGGATACGGCTATTCTTACAATCCCGACTA
Protein Sequence	>YP_002745845.1 fibronectin-binding protein Sfs [Streptococcus equi subsp. equi 4047] MRKTEGRFRTWKSKKQWLFAGAVVTSLLLGAALVFGGLLGSLGGSSHQARPKEQPVSSIGDDDKSHKSSS DSMVSRPPKKDNLQPKPSDQPTNHQHQATSPSQPTAKSSGHHGNQPQSLSVNSQGNSSGQASEPQAIPNQ GPSQPLGLRGGNSSGSGHHHQPQGKPQHLDLGKDNSSPQPQPKPQGNSPKLPEKGLNGENQKEPEQGERG EAGPPLSGLSGNNQGRPSLPGLNGENQKEPEQGERGEAGPPSTPNLEGNNRKNPLKGLDGENKPKEDLDG KGLSGENDESPKLKDEHPYNHGRRDGYRVGYEDGYGGKKHKGDYPKRFDESSPKEYNDYSQGYNDNYGNG YLDGLADRGGKRGYGYSYNPD
Molecule Role	Protective antigen
Molecule Role Annotation	Parts of the three recombinant proteins (FNZ (cell surface-bound fibronectin binding protein), SFS (secreted fibronectin binding protein), and EAG (alpha2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein)) were used to immunize mice, either subcutaneously or intranasally, prior to nasal challenge with S. equi subsp. equi. The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin. It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) (Flock et al., 2004).
Related Vaccines(s)	S. equi Subunit EAG, FNZ and SFS Protein Vaccine
References

Sfs