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YopD from Y. pestis CO92
Gene Name	YopD from Y. pestis CO92
Sequence Strain (Species/Organism)	Yersinia pestis CO92
VO ID	VO_0010878
NCBI Gene ID	1172673
NCBI Protein GI	16082716
Locus Tag	YPCD1.28c
Genbank Accession	AL117189
Protein Accession	NP_395162
Taxonomy ID	214092
Plasmid No	pCD1
Gene Starting Position	19293
Gene Ending Position	20213
Gene Strand (Orientation)	-
Protein Name	secreted effector protein
Protein pI	6.98
Protein Weight	31501.57
Protein Length	306
Protein Note	Yop targeting negative regulator; translocon component; important for translocation pore formation
DNA Sequence	>NC_003131.1:19293-20213 Yersinia pestis CO92 plasmid pCD1, complete sequence GTCAGACAACACCAAAAGCGGCTTTCATGGCGTGAGTATGACTGCTAACATATTGTTCAATCAAGCGCAG GACATCTTTCATAAAGTTATCATTATAGTTCATCGCCTCTTCGGCTTTTTGCTTCTCGTTGGCAGCAATA CTTGCATTGACTTCTTTCTCTTTGACCTCGGCTTGAGAATACCCTTGATGAACTTGAATCGCACTGTTTG CCATTTGTCCTAACGGCTGCGTTGCGGCATTAAACGCATTGGCTTTTGAGTCGGTCATTCTGAATTCTTT ATCCAATAATGCCAGCTTATTTTGATCCGCTACCTGCTCTGGTTTCCATATTCTCCCGATATCCTCTCTG CTTACCGCTTTATCACCAATGTTACTCATTTGCTGCATTTTTGTATCAATAAGTTGTTCGCGGCCAGCAA TATTACTGTTTAGAATTTGTTCCTGTTTAACTATTTTCACCTCTTTCGCTATAGAAAAAGCACTAGCAAC CGTAGAAGTAGCAGCCATGATGCCAGACACCACCGCCATGGCGATCATCAGTTTTGCACCGCTGACCATC TCCGCTACCTGCTCCTTTTGGGCAGTAATAGTAGCTTTATTTTCTATATCCCTTTGTTGCAAACCCATTT CTCGCGCTTTACGTGCCAGTTCCAACAGCAACAACAAGATACTTAAAGTACCATTAAGATCACCCTGGCT TTTACTCAGTAATGCAACATTGATTCCCTGGCTCGGTTTGATCAATTCTGGCACCTGTGACCGAGATAAG CTTGCCTCGCTACTCTTTATTGCTTGTGCTTCATGACGGGTGTCTTCTGTTTTTTTAATCTCACCGCTTT GCTTGACTGTCTCTGTAGTGATGGCATCAAGCTGTGAACCGGTCGTGATAATTGGGCTGTCTGTCTTGAT ATTTATTGTCA
Protein Sequence	>NP_395162.1 secreted effector protein (plasmid) [Yersinia pestis CO92] MTINIKTDSPIITTGSQLDAITTETVKQSGEIKKTEDTRHEAQAIKSSEASLSRSQVPELIKPSQGINVA LLSKSQGDLNGTLSILLLLLELARKAREMGLQQRDIENKATITAQKEQVAEMVSGAKLMIAMAVVSGIMA ATSTVASAFSIAKEVKIVKQEQILNSNIAGREQLIDTKMQQMSNIGDKAVSREDIGRIWKPEQVADQNKL ALLDKEFRMTDSKANAFNAATQPLGQMANSAIQVHQGYSQAEVKEKEVNASIAANEKQKAEEAMNYNDNF MKDVLRLIEQYVSSHTHAMKAAFGVV
Molecule Role	Protective antigen
Molecule Role Annotation	The type III secretion apparatus encoded on the low-calcium response (LCR) virulence plasmid, pCD1 in strain KIM5, of Y. pestis is a conserved virulence mechanism that is absolutely required for virulence of Y. pestis. YscF is a surface localized protein required both to secrete Yops and to translocate toxins into eukaryotic cells. YscF polymerization is possibly required for a YscF needle to puncture eukaryotic cell membranes. It is also likely that YscF and its homologs function to provide a base that a translocon complex is built upon, or that YscF builds a conduit from the bacterium to the eukaryotic membrane - this suggestion seems more likely given that other proteins such as YopB, YopD, and LcrV are also required for translocation into eukaryotic cells. YscF is thought to be a surface-expressed protein in Y. pestis and is required for virulence. YscF has been found to function as a protective antigen against experimental plague infection (Matson et al., 2005).
Related Vaccines(s)	Recombinant Y. pestis YopD protein vaccine
References

YopD from Y. pestis CO92