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BoNT/B

Gene Name BoNT/B
Sequence Strain (Species/Organism) Clostridium botulinum B1 str. Okra
VO ID VO_0010904
NCBI Gene ID 6030102
NCBI Protein GI 169834607
Locus Tag CLD_A0068
Genbank Accession AB232927
Protein Accession YP_001693307
3D structure: PDB ID 1S0F
Other Database IDs CDD:279999
CDD:285227
CDD:285228
CDD:285226
Taxonomy ID 498213
Plasmid No pCLD
Gene Starting Position 42316
Gene Ending Position 46191
Gene Strand (Orientation) -
Protein Name botulinum neurotoxin type B
Protein pI 4.97
Protein Weight 145339.54
Protein Length 1291
Protein Note Clostridial neurotoxin zinc protease; pfam01742
DNA Sequence 
>NC_010379.1:42316-46191 Clostridium botulinum B1 str. Okra plasmid pCLD, complete sequence
ATTATTCAGTCCACCCTTCATCTTTAGGAATAAACTGCCAATTACATCCCAATTTTAAATTATATGGTTT
CCTTTTTACCTCTTTTAAGTACCATTTACTTATACAAAAATAATCTTTATACTCTTCAAATACAATTCCA
GATTCGTAGAAACGATGAATACCAATCAATCCTATCTCATCAGTACTTTCTTCATCTTTTTTAAAAAGCA
ACTGACAACTATATGTTGGCTGTTCATCATATTCTTTTATTTGTATAGTATTGTAAAACTCATCAGAATC
ACTTATAGGAGCTAAAAACAATTTTTCTTCCTCTTTCTTAAAATATTTATAGGTATATACTCTCCACTCT
TGATTTAAATTAAAAAAATCTAGATATATATAATCTTCTTTTCTAACTATATCATCATTTATAGATTGAG
AATTTGACTTTCTTCTTATAATAAATTTTTCTCCAATATATAAATCTCTATAATTTATATATTTAGAATT
TTGATTATATTTGCTACGTGTTAAAATTTCACCTACAGGTGAATCTTTCTTTAGTTTAATATATGAATTT
TTATTCCCCGCATTAAACATATAATATTCTTTATTGTACATTAAAGGATTTCCCCAAAAATCTTTTAAAT
ATTCGCTATATGATTGAATTTTATATCTTTCTTCAATATTTGATTGACTTAATTCCGTATTAAAAATACT
GAAATATTTCATCCAAATAAATTGTGTTCTATCTATATCACCATCTAATTTAAATATTATTTCACCATTA
GCAATAACTTCTCTTATATCTTTAATATCTGTATTTGATTCTAGCTTACCATTAATATAAATTTTAGCGT
TATTCAAATTATTAGTAATAGTTACAAAAAACCATCTATTTATATACTCTGATATATCTTCTCTTATGTT
ATATTCAAAAAATACCGATTTGGTTTTTCCATTTATATCAATTAAAGTCCATATTATCCTATTACCCCTA
ATAGATATTTTCCAGCCCGAATTATTTTTCATACAATTAATTATTGTATATTCATTATGAATATAATTTT
GTATACCATCATTCTTATATTTAGGTATTCTTATCCAAAAGCTAACGCTAAAATCAAGGAACACACTATT
AAATATGATATTCTGATTTTGAGTCACTCTAATCTTACTATTTGCTGAACTAGTTAATTTAAATTGATTT
TTATCATTAAGCTCGACTCCATCATATACCTCTACCTTTGCCCCATATCCTGATAAATCTATTAAATTAT
TATCCTTATATCTTAAATTTAAGATAATATTATTTAAAATTTCGCTATTATATTTATTAAACATTTCTAT
TAGTATTGTATCATTGGTATATATTGAAAGATCAAACGGCATAATGGTTTTCAAGTATTTATTTACTTTT
GATTTTTCATATTCTGCACTTCCAATCAAATATAATTTATTTTCATCTATATAATTTAACAAATTTTTTT
TGAGAGTATTATCAAAGTCTAGTAATTTTTCTACAGCTAATGGAATCATTTTTTTCATTAAATATGATAC
AGAACATCCATTTATAAAATTATTTATATTATCTATAGCTTGGTTAATACCCTCATTAAGTTTAGAATTT
ATATCATTAAAATCGATGTTAATATTTGACTTTTCTTTTTCAGAATATATATTATATCTGTATTTTATTA
TTTCTTCCAATGCTTGTGCTTGATAATTTAAAGCCTTATACATTCCCTCTTTTATTGTATAAAATTGAGT
ATTAACTGTTGAGAGCCATTGCGCTACTATTAATCCGTACATATCACTCCATTTTTCATTTCTTTTAGTT
AAAGCATTATCTATTGTTTTAATAATTTTATTTTTATTGTCAATATATGATTCTAATAAAAAGGCTCCAA
CTACAGGTATTAAAAGTTCTGGTATAAATTCTAGTAGAATACTGGCTCCTGCAATCTCAAAAGCATTTTC
AAAATTTCCTTTAGCTGTTTCATTTCCTACATTTAAAGCTAATCCTATATAAGGAACAATTAGAGATATA
TCTGCAATTTTATCCATAGTATTGCTTTTATTAGCTTCGATTACAAAATCATTTACTATCTGTTTCACCC
AACCTGCAAATAATCCTGCTTCTACCACTTTATTAGCAGTTTTAATATAATCCATAGAAAAAAATGAATA
AACTTTGTTAGAAAATAATAATGCATCATCAAATGAAGATGTTAAACTTATATCTCTTATATCTAGAGGA
AATGTCTGAGAGTATAAATATTGAAAGATGGTATTTTCATCTGTAAAAATTTTTTTTATAGCGGGTTGTT
TTTCATATACTGGAACATCTACATTAAAATCAGTAAGTGATTCTGTATTTTCACTTGGTAATTCTATTTT
ACTTATTAAATCAGTATCTAAAATTAATTCATTTATAGGGAAGTCATTTTCTATATAATTACTCTGTGTA
TTATATTCTATTCTTTCGTTTTTAGATAAATCATCTGAAAAACTATTTTTATCAGCTATAAAGAACAAAT
CTTCATTATCAACATCAATACATATTCCTGGAGCTTTAACACTTTTACACATTTGTATCTTATATACAGC
CAAATGCTCCTTGCTAATTTCTTCATAAGCTTGTTTATTTATAGCTTTATTCTGACCTCTATATTCTTTT
TCCATATCTTTATCAGATATATTAAACCCTTCCTCTATAGTATAGATTTCATTATCTAATAAATTTTTTA
TTTTTACTGGTGGTAAGGAATCACTAAAATAAGAAGCTCTAGTTTTTATTTTATAATTTTCTGCTATATT
AGTTTCTGTAAAACCAAACATTAAGCTTTTATATAATTTATCAAAACTTTCTACATCTATACTATATTTT
CCCTCAGAATCTTCAACGAATTTATATTTATCTTTAAATTTATTTTTATATATATTAATATTAATGTTAG
GATCTGATATGCAAACTAAAACCTTGTTAAGTCTATCAACTATCCCTCTAAAATTTTGCAAAACTTTATC
ATAGATACTTTTATCCGTAGAAGGAGTTATGATGCTGGGATCTTGTCCTCCAAATGTATATAGTTCTTCT
GCCTGTATAGCATCTGTAGATTGCATAAAAAATTTTTTTTCATTTGGTACAATTGGTAAATCATCTACTT
TAATGCCATATAATCCATGTAAAACATGTATAAGTTCATGCATTAATATCAAGGCTGGATCTGAAAAATA
TCCACGTCTATTAAATATACTTGCGCCTTTGTTTTCTTGAACATTATTAAATACGCTTACATATTCTGGG
CAAAACTTCATTTGCATTATACCCCCGAAGCCTTCCCTTGATGCAAAATGATTTTGTATACCTATATCTA
TAGTCTCATTTTCATTTAAAACTGGCCCAGGTCCAAATATTATTAAATTTGCGAAAATACCTTTTTTTCG
CTCCACTTCTCCTGGATTACTGATTAATTTATTAACAGTTACACTAGCAATGTTTGTGTTAAACTCTTCG
AGTGGAACACGTCTATCTCCAAGATAAGGTATACCATTTATAATCATCTCTAATAACTTTTCACCCAATG
GTTTTGATTTGATTCTATTAAATAACTTGATCATTGTTTGTAAAAATATATTCTTTTTATCATTAGTATT
TAAGTAATCTGGATCATAATATTCACAAACATCTCTATTAAAAATACCGGAACTTTTATTAAAATCCTCA
GGTTTATATCCAAAAGTATATCTTTCCGGTATTATCCAAATACGATCTGTGATTTTAAAAGCTTTATAAT
ATCTCCCCGTACCTCTCGCAAATGGAGGCTCCATCATAATAATATTATTATTATCAATAGGATCATTATA
ATTAAAATTATTTATTGTAACTGGCA
Protein Sequence 
>YP_001693307.1 botulinum neurotoxin type B (plasmid) [Clostridium botulinum B1 str. Okra]
MPVTINNFNYNDPIDNNNIIMMEPPFARGTGRYYKAFKITDRIWIIPERYTFGYKPEDFNKSSGIFNRDV
CEYYDPDYLNTNDKKNIFLQTMIKLFNRIKSKPLGEKLLEMIINGIPYLGDRRVPLEEFNTNIASVTVNK
LISNPGEVERKKGIFANLIIFGPGPVLNENETIDIGIQNHFASREGFGGIMQMKFCPEYVSVFNNVQENK
GASIFNRRGYFSDPALILMHELIHVLHGLYGIKVDDLPIVPNEKKFFMQSTDAIQAEELYTFGGQDPSII
TPSTDKSIYDKVLQNFRGIVDRLNKVLVCISDPNININIYKNKFKDKYKFVEDSEGKYSIDVESFDKLYK
SLMFGFTETNIAENYKIKTRASYFSDSLPPVKIKNLLDNEIYTIEEGFNISDKDMEKEYRGQNKAINKQA
YEEISKEHLAVYKIQMCKSVKAPGICIDVDNEDLFFIADKNSFSDDLSKNERIEYNTQSNYIENDFPINE
LILDTDLISKIELPSENTESLTDFNVDVPVYEKQPAIKKIFTDENTIFQYLYSQTFPLDIRDISLTSSFD
DALLFSNKVYSFFSMDYIKTANKVVEAGLFAGWVKQIVNDFVIEANKSNTMDKIADISLIVPYIGLALNV
GNETAKGNFENAFEIAGASILLEFIPELLIPVVGAFLLESYIDNKNKIIKTIDNALTKRNEKWSDMYGLI
VAQWLSTVNTQFYTIKEGMYKALNYQAQALEEIIKYRYNIYSEKEKSNINIDFNDINSKLNEGINQAIDN
INNFINGCSVSYLMKKMIPLAVEKLLDFDNTLKKNLLNYIDENKLYLIGSAEYEKSKVNKYLKTIMPFDL
SIYTNDTILIEMFNKYNSEILNNIILNLRYKDNNLIDLSGYGAKVEVYDGVELNDKNQFKLTSSANSKIR
VTQNQNIIFNSVFLDFSVSFWIRIPKYKNDGIQNYIHNEYTIINCMKNNSGWKISIRGNRIIWTLIDING
KTKSVFFEYNIREDISEYINRWFFVTITNNLNNAKIYINGKLESNTDIKDIREVIANGEIIFKLDGDIDR
TQFIWMKYFSIFNTELSQSNIEERYKIQSYSEYLKDFWGNPLMYNKEYYMFNAGNKNSYIKLKKDSPVGE
ILTRSKYNQNSKYINYRDLYIGEKFIIRRKSNSQSINDDIVRKEDYIYLDFFNLNQEWRVYTYKYFKKEE
EKLFLAPISDSDEFYNTIQIKEYDEQPTYSCQLLFKKDEESTDEIGLIGIHRFYESGIVFEEYKDYFCIS
KWYLKEVKRKPYNLKLGCNWQFIPKDEGWTE
Molecule Role Protective antigen
Molecule Role Annotation The DNA replicon vaccine (pSCARSBHc) encoding the Hc domain of BoNT/B (BHc) induced better responses and protection against BoNT/B mouse challenge than conventional DNA vaccine. Recombinant SFV particle VRP-BHc protected mice from high-dose BoNT/B challenge (Yu et al., 2009).
The production of ciBoNT/B1 HP, ciBoNT/C1 HP, ciBoNT/E1 HP and ciBoNT/F1 HP suggest the ciBoNT HP vaccines exhibit superior potency after single vaccinations.(Webb et al., 2017)
Related Vaccines(s) C. botulinum DNA vaccine pSCARSA/BHc
References