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BoNT/A1 from C. botulinum A str. Hall
Gene Name	BoNT/A1 from C. botulinum A str. Hall
Sequence Strain (Species/Organism)	Clostridium botulinum A str. Hall
VO ID	VO_0010903
NCBI Gene ID	5398487
NCBI Protein GI	153936924
Locus Tag	CLC_0862
Genbank Accession	CP000727
Protein Accession	YP_001386738
3D structure: PDB ID	3NF3
Taxonomy ID	441771
Gene Starting Position	882202
Gene Ending Position	886092
Gene Strand (Orientation)	+
Protein Name	botulinum neurotoxin type A1
Protein pI	6.31
Protein Weight	142277.54
Protein Length	1296
Protein Note	identified by similarity to SP:P10845; similarity to SP:P30996; match to protein family HMM PF01742; match to protein family HMM PF07951; match to protein family HMM PF07952; match to protein family HMM PF07953
DNA Sequence	>NC_009698.1:882202-886092 Clostridium botulinum A str. Hall chromosome, complete genome TATGCCATTTGTTAATAAACAATTTAATTATAAAGATCCTGTAAATGGTGTTGATATTGCTTATATAAAA ATTCCAAATGCAGGACAAATGCAACCAGTAAAAGCTTTTAAAATTCATAATAAAATATGGGTTATTCCAG AAAGAGATACATTTACAAATCCTGAAGAAGGAGATTTAAATCCACCACCAGAAGCAAAACAAGTTCCAGT TTCATATTATGATTCAACATATTTAAGTACAGATAATGAAAAAGATAATTATTTAAAGGGAGTTACAAAA TTATTTGAGAGAATTTATTCAACTGATCTTGGAAGAATGTTGTTAACATCAATAGTAAGGGGAATACCAT TTTGGGGTGGAAGTACAATAGATACAGAATTAAAAGTTATTGATACTAATTGTATTAATGTGATACAACC AGATGGTAGTTATAGATCAGAAGAACTTAATCTAGTAATAATAGGACCCTCAGCTGATATTATACAGTTT GAATGTAAAAGCTTTGGACATGAAGTTTTGAATCTTACGCGAAATGGTTATGGCTCTACTCAATACATTA GATTTAGCCCAGATTTTACATTTGGTTTTGAGGAGTCACTTGAAGTTGATACAAATCCTCTTTTAGGTGC AGGCAAATTTGCTACAGATCCAGCAGTAACATTAGCACATGAACTTATACATGCTGGACATAGATTATAT GGAATAGCAATTAATCCAAATAGGGTTTTTAAAGTAAATACTAATGCCTATTATGAAATGAGTGGGTTAG AAGTAAGCTTTGAGGAACTTAGAACATTTGGGGGACATGATGCAAAGTTTATAGATAGTTTACAGGAAAA CGAATTTCGTCTATATTATTATAATAAGTTTAAAGATATAGCAAGTACACTTAATAAAGCTAAATCAATA GTAGGTACTACTGCTTCATTACAGTATATGAAAAATGTTTTTAAAGAGAAATATCTCCTATCTGAAGATA CATCTGGAAAATTTTCGGTAGATAAATTAAAATTTGATAAGTTATACAAAATGTTAACAGAGATTTACAC AGAGGATAATTTTGTTAAGTTTTTTAAAGTACTTAACAGAAAAACATATTTGAATTTTGATAAAGCCGTA TTTAAGATAAATATAGTACCTAAGGTAAATTACACAATATATGATGGATTTAATTTAAGAAATACAAATT TAGCAGCAAACTTTAATGGTCAAAATACAGAAATTAATAATATGAATTTTACTAAACTAAAAAATTTTAC TGGATTGTTTGAATTTTATAAGTTGCTATGTGTAAGAGGGATAATAACTTCTAAAACTAAATCATTAGAT AAAGGATACAATAAGGCATTAAATGATTTATGTATCAAAGTTAATAATTGGGACTTGTTTTTTAGTCCTT CAGAAGATAATTTTACTAATGATCTAAATAAAGGAGAAGAAATTACATCTGATACTAATATAGAAGCAGC AGAAGAAAATATTAGTTTAGATTTAATACAACAATATTATTTAACCTTTAATTTTGATAATGAACCTGAA AATATTTCAATAGAAAATCTTTCAAGTGACATTATAGGCCAATTAGAACTTATGCCTAATATAGAAAGAT TTCCTAATGGAAAAAAGTATGAGTTAGATAAATATACTATGTTCCATTATCTTCGTGCTCAAGAATTTGA ACATGGTAAATCTAGGATTGCTTTAACAAATTCTGTTAACGAAGCATTATTAAATCCTAGTCGTGTTTAT ACATTTTTTTCTTCAGACTATGTAAAGAAAGTTAATAAAGCTACGGAGGCAGCTATGTTTTTAGGCTGGG TAGAACAATTAGTATATGATTTTACCGATGAAACTAGCGAAGTAAGTACTACGGATAAAATTGCGGATAT AACTATAATTATTCCATATATAGGACCTGCTTTAAATATAGGTAATATGTTATATAAAGATGATTTTGTA GGTGCTTTAATATTTTCAGGAGCTGTTATTCTGTTAGAATTTATACCAGAGATTGCAATACCTGTATTAG GTACTTTTGCACTTGTATCATATATTGCGAATAAGGTTCTAACCGTTCAAACAATAGATAATGCTTTAAG TAAAAGAAATGAAAAATGGGATGAGGTCTATAAATATATAGTAACAAATTGGTTAGCAAAGGTTAATACA CAGATTGATCTAATAAGAAAAAAAATGAAAGAAGCTTTAGAAAATCAAGCAGAAGCAACAAAGGCTATAA TAAACTATCAGTATAATCAATATACTGAGGAAGAGAAAAATAATATTAATTTTAATATTGATGATTTAAG TTCGAAACTTAATGAGTCTATAAATAAAGCTATGATTAATATAAATAAATTTTTGAATCAATGCTCTGTT TCATATTTAATGAATTCTATGATCCCTTATGGTGTTAAACGGTTAGAAGATTTTGATGCTAGTCTTAAAG ATGCATTATTAAAGTATATATATGATAATAGAGGAACTTTAATTGGTCAAGTAGATAGATTAAAAGATAA AGTTAATAATACACTTAGTACAGATATACCTTTTCAGCTTTCCAAATACGTAGATAATCAAAGATTATTA TCTACATTTACTGAATATATTAAGAATATTATTAATACTTCTATATTGAATTTAAGATATGAAAGTAATC ATTTAATAGACTTATCTAGGTATGCATCAAAAATAAATATTGGTAGTAAAGTAAATTTTGATCCAATAGA TAAAAATCAAATTCAATTATTTAATTTAGAAAGTAGTAAAATTGAGGTAATTTTAAAAAATGCTATTGTA TATAATAGTATGTATGAAAATTTTAGTACTAGCTTTTGGATAAGAATTCCTAAGTATTTTAACAGTATAA GTCTAAATAATGAATATACAATAATAAATTGTATGGAAAATAATTCAGGATGGAAAGTATCACTTAATTA TGGTGAAATAATCTGGACTTTACAGGATACTCAGGAAATAAAACAAAGAGTAGTTTTTAAATACAGTCAA ATGATTAATATATCAGATTATATAAACAGATGGATTTTTGTAACTATCACTAATAATAGATTAAATAACT CTAAAATTTATATAAATGGAAGATTAATAGATCAAAAACCAATTTCAAATTTAGGTAATATTCATGCTAG TAATAATATAATGTTTAAATTAGATGGTTGTAGAGATACACATAGATATATTTGGATAAAATATTTTAAT CTTTTTGATAAGGAATTAAATGAAAAAGAAATCAAAGATTTATATGATAATCAATCAAATTCAGGTATTT TAAAAGACTTTTGGGGTGATTATTTACAATATGATAAACCATACTATATGTTAAATTTATATGATCCAAA TAAATATGTCGATGTAAATAATGTAGGTATTAGAGGTTATATGTATCTTAAAGGGCCTAGAGGTAGCGTA ATGACTACAAACATTTATTTAAATTCAAGTTTGTATAGGGGGACAAAATTTATTATAAAAAAATATGCTT CTGGAAATAAAGATAATATTGTTAGAAATAATGATCGTGTATATATTAATGTAGTAGTTAAAAATAAAGA ATATAGGTTAGCTACTAATGCATCACAGGCAGGCGTAGAAAAAATACTAAGTGCATTAGAAATACCTGAT GTAGGAAATCTAAGTCAAGTAGTAGTAATGAAGTCAAAAAATGATCAAGGAATAACAAATAAATGCAAAA TGAATTTACAAGATAATAATGGGAATGATATAGGCTTTATAGGATTTCATCAGTTTAATAATATAGCTAA ACTAGTAGCAAGTAATTGGTATAATAGACAAATAGAAAGATCTAGTAGGACTTTGGGTTGCTCATGGGAA TTTATTCCTGTAGATGATGGATGGGGAGAAAGGCCACTGTA
Protein Sequence	>YP_001386738.1 botulinum neurotoxin type A1 [Clostridium botulinum A str. Hall] MPFVNKQFNYKDPVNGVDIAYIKIPNAGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLNPPPEAKQVPV SYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGGSTIDTELKVIDTNCINVIQP DGSYRSEELNLVIIGPSADIIQFECKSFGHEVLNLTRNGYGSTQYIRFSPDFTFGFEESLEVDTNPLLGA GKFATDPAVTLAHELIHAGHRLYGIAINPNRVFKVNTNAYYEMSGLEVSFEELRTFGGHDAKFIDSLQEN EFRLYYYNKFKDIASTLNKAKSIVGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYT EDNFVKFFKVLNRKTYLNFDKAVFKINIVPKVNYTIYDGFNLRNTNLAANFNGQNTEINNMNFTKLKNFT GLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDLNKGEEITSDTNIEAA EENISLDLIQQYYLTFNFDNEPENISIENLSSDIIGQLELMPNIERFPNGKKYELDKYTMFHYLRAQEFE HGKSRIALTNSVNEALLNPSRVYTFFSSDYVKKVNKATEAAMFLGWVEQLVYDFTDETSEVSTTDKIADI TIIIPYIGPALNIGNMLYKDDFVGALIFSGAVILLEFIPEIAIPVLGTFALVSYIANKVLTVQTIDNALS KRNEKWDEVYKYIVTNWLAKVNTQIDLIRKKMKEALENQAEATKAIINYQYNQYTEEEKNNINFNIDDLS SKLNESINKAMININKFLNQCSVSYLMNSMIPYGVKRLEDFDASLKDALLKYIYDNRGTLIGQVDRLKDK VNNTLSTDIPFQLSKYVDNQRLLSTFTEYIKNIINTSILNLRYESNHLIDLSRYASKINIGSKVNFDPID KNQIQLFNLESSKIEVILKNAIVYNSMYENFSTSFWIRIPKYFNSISLNNEYTIINCMENNSGWKVSLNY GEIIWTLQDTQEIKQRVVFKYSQMINISDYINRWIFVTITNNRLNNSKIYINGRLIDQKPISNLGNIHAS NNIMFKLDGCRDTHRYIWIKYFNLFDKELNEKEIKDLYDNQSNSGILKDFWGDYLQYDKPYYMLNLYDPN KYVDVNNVGIRGYMYLKGPRGSVMTTNIYLNSSLYRGTKFIIKKYASGNKDNIVRNNDRVYINVVVKNKE YRLATNASQAGVEKILSALEIPDVGNLSQVVVMKSKNDQGITNKCKMNLQDNNGNDIGFIGFHQFNNIAK LVASNWYNRQIERSSRTLGCSWEFIPVDDGWGERPL
Molecule Role	Protective antigen
Molecule Role Annotation	Mice and rabbits were immunized with recombinant HCR/A1 (rHCR/A1) from the classical type A-Hall strain (ATCC 3502) (BoNT/A1) and rHCR/E from BoNT serotype E Beluga (BoNT/E(B)). The protection elicited by rHCR/A1 to BoNT/A1 and BoNT/A2 and by rHCR/E(B) to BoNT/E(A) indicate that immunization with receptor binding domains elicit protection within sub-serotypes of BoNT (Baldwin et al., 2005).
Related Vaccines(s)	C. botulinum DNA vaccine pSCARSA/BHc
References

BoNT/A1 from C. botulinum A str. Hall