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GroEL
Gene Name	GroEL
Sequence Strain (Species/Organism)	Salmonella enterica subsp. enterica serovar Typhi str. Ty2
VO ID	VO_0010998
NCBI Gene ID	1068458
NCBI Protein GI	29144631
Locus Tag	t4382
Genbank Accession	AE014613
Protein Accession	NP_807973
Taxonomy ID	209261
Gene Starting Position	4534506
Gene Ending Position	4536152
Gene Strand (Orientation)	+
Protein Name	molecular chaperone GroEL
Protein pI	4.57
Protein Weight	51532.68
Protein Length	548
Protein Note	60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth
DNA Sequence	>NC_004631.1:4534506-4536152 Salmonella enterica subsp. enterica serovar Typhi Ty2, complete genome AATGGCAGCTAAAGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTG GCAGATGCAGTGAAAGTAACCCTCGGTCCGAAAGGCCGTAACGTGGTTCTGGATAAATCTTTCGGTGCGC CGACTATCACTAAAGATGGTGTTTCCGTAGCGCGTGAAATCGAGCTGGAAGACAAGTTTGAAAACATGGG CGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCGAACGACGCTGCAGGCGACGGCACCACCACCGCGACC GTACTGGCGCAGTCCATCATTACCGAAGGCCTGAAAGCCGTTGCTGCGGGCATGAACCCGATGGACCTGA AACGTGGTATCGACAAAGCGGTTGCTGCTGCGGTTGAAGAGCTGAAGGCGCTGTCCGTACCGTGCTCCGA CTCTAAAGCGATTGCTCAGGTAGGTACTATTTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCG GAAGCGATGGATAAAGTCGGTAAAGAAGGCGTCATCACTGTTGAAGACGGTACCGGTCTGCAGGACGAAC TGGACGTGGTTGAAGGTATGCAGTTTGACCGCGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAAC TGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGATAAGAAAATCTCCAACATCCGCGAAATG CTGCCGGTTCTGGAAGCCGTTGCAAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTTGAAGGCG AAGCGCTGGCTACCCTGGTAGTGAACACCATGCGTGGCATCGTGAAAGTGGCTGCGGTTAAAGCACCGGG CTTCGGCGATCGTCGTAAGGCGATGCTGCAGGATATCGCTACCCTGACCGGCGGTACCGTAATCTCTGAA GAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCGAAACGTGTTGTGATCAACA AAGACACCACCACCATCATTGATGGCGTGGGTGAAGAAGCTGCCATCCAGGGCCGTGTTGCTCAGATCCG TCAGCAGATTGAAGAAGCGACCTCCGACTACGATCGTGAAAAACTGCAGGAGCGCGTAGCGAAACTGGCA GGCGGCGTTGCGGTAATCAAAGTTGGCGCTGCGACCGAAGTTGAAATGAAAGAGAAGAAAGCCCGCGTTG AAGATGCCCTGCACGCGACCCGTGCTGCGGTAGAAGAAGGCGTGGTTGCTGGTGGTGGCGTTGCGCTGAT CCGCGTTGCTTCTAAAATTGCTGACCTGAAAGGCCAGAACGAAGACCAGAACGTGGGTATCAAAGTTGCG CTGCGCGCAATGGAAGCTCCGCTGCGTCAGATCGTGCTGAACTGCGGCGAAGAGCCGTCTGTTGTCGCTA ACACCGTTAAAGGCGGCGACGGTAACTACGGTTACAACGCAGCAACTGAAGAATACGGCAACATGATCGA TATGGGTATCTTGGACCCAACCAAAGTTACCCGTTCTGCGCTGCAATACGCGGCTTCTGTGGCTGGTCTG ATGATCACTACCGAGTGCATGGTGACCGACCTGCCGAAAAGCGATGCTCCTGATTTAGGCGCTGCTGGCG GCATGGGTGGTATGGGTGGTATGGGCGGCATGATGTA
Protein Sequence	>NP_807973.1 molecular chaperone GroEL [Salmonella enterica subsp. enterica serovar Typhi str. Ty2] MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMG AQMVKEVASKANDAAGDGTTTATVLAQSIITEGLKAVAAGMNPMDLKRGIDKAVAAAVEELKALSVPCSD SKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPET GAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPG FGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIR QQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALI RVASKIADLKGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMID MGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKSDAPDLGAAGGMGGMGGMGGMM
Molecule Role	Protective antigen
Molecule Role Annotation	Researchers evaluated the immunogenicity and protective efficacy of GroEL of Salmonella enterica serovar Typhi against lethal infection by S. typhi Ty2 in mice with or without adjuvants. Immunization of mice with GroEL-alone, GroEL + alum, and GroEL + CFA provided 70, 50 and 80% protection, respectively, against lethal challenge by S. typhi in mice. The present study shows that GroEL forms an ideal candidate molecule to develop a recombinant protein based vaccine against human typhoid (Bansal et al., 2010).
Related Vaccines(s)	S. typhi GroEL Protein Vaccine
References

GroEL