VIOLIN: Vaccine Investigation and Online Information Network

H2-K1
Gene Name	H2-K1
Sequence Strain (Species/Organism)	Mus musculus
NCBI Gene ID	14972
NCBI Protein GI	133922588
Genbank Accession	AB114280
Protein Accession	NP_001001892
Taxonomy ID	10090
Chromosome No	17
Gene Starting Position	33996011
Gene Ending Position	34000340
Gene Strand (Orientation)	-
Protein Name	transcript variant X11
Protein pI	6.35
Protein Weight	38523.57
Protein Length	369
Protein Note	Also known as K-f; H-2K; H2-K; H-2K(d); MGC7052; MGC184092
DNA Sequence	>NC_000083.6:33996011-34000340 Mus musculus strain C57BL/6J chromosome 17, GRCm38.p4 C57BL/6J ATAGTGACCCAGATTCTGGAAGTTTATTCATCTATCATTTATTTCTTCAAAAAAACAAATCAATCAAACA AACAAACAAAACCTCTAAGCATTCTCAATCCATGAAATTAACAAGAAATCAGCCCTAGGTCAAGATGATA ACAATCAAGGTTACATTCAAATTATTATTCTCAGTGTGGAAGTGAGGAGTTGCAGGTCAGGGTAGCATGG AGCTGACAGGCTACAGATGTCCCTCAGTGTTTGGCTGAACCAGCAAGGTTGGCTGTGGAAGGGAAGACAG AGCAGTGCAGGGACAGGGTCCTGGTGTAGAGGGGTGGACTGGGCTCCACAGTTCTTCACATTGAGTCCAT AGGCTCACAGGGAACATCAGACACTTGACTAAAGAGAACTGAGGGCTCTGGATGTCACAGGAGATATGAG AAGACATTGTCTGTCACCAAGTCCACTCCAGGCAGCTGTCTTCACGCTAGAGAATGAGGGTCATGAACCA TCACTGTGAAGACAACATTCGAACAACCCATCACTCACTCACAGGGGATTCTGGGAGTTCCTGAAACCCA ATCATGTCCACATTGCCCCTCCCCAATCAGACCCTAGAGTGTCACCTTTACAATCTGGGAGAGACAGATC AGAGGTCTGGGAGCCTAGGGTAAAACAAAACAAATGTATATGCATATGTACATGTACATATTCATTTCAT GGCACAACTCAGGCACCTAGAAGATTTCTCTGGAGGAGCTATTATGGATTCCCAGAGCTCCCATCATCTC CAACTTCACTCCAATGTCCCCAGGACAACTCTGTCCCCACACTAACCTGGAGCCAGAGCATAGTCCCCTC CTTTTCCACCTGTGAGAAGAAAAGCTGTGAGAGTTCCAGGAAGATCTTGACACTAGGAAGTTCCCAGAAC TGACAGCAGACCCAGTTAGAGACAGTAGCAATGTGGGGTGTGCCTGTGTTCCCCATTGATGAGCAGAGCA CACTCTAAAGGAGGCTGAGAGAAAACTCAGACTCTGCCCTTTCCTACCTGTGTTTCTCCTTCTCATCTTC ATCACAAAAGCCACCACAGCTCCAGTGACTATTGCAGCTCCAAGGACAACCAGAACAGCAACGGTCGCCA TGTTGGAGACAGTGGATGGAGGAGGCTCTGGGAAGGACAGGTACAAGAAATGAAGGTGAGGGTCATGACC CCAGCTCTCAGCCCTGAGCAGCTCAGGGTCTGCAGAAAGCTCCAGCTTTCCCTGACCCCAGCTCTGCACC CACACTCTCCTTACCCCATCTCAGGGTGAGGGGCTCAGGCAGCCCCTGATGGTACACATGGCATGTGTAA TACTGCTCCTTCCCAAGAGGCACCACCACAGATGCCCACTTCTGGAAGGTTCCATCCCCTGCAGGCCTGG TCTCCACAAGCTCCATGTCCTGGATCAGCTCCTCCCCATTCAACTGCCAGGTCAGGGTGATGTCAGCAGG GTAGAAGCCCAGGGCCCAGCACCTCAGGGTGACTTTATCTTCAGGTCTGCTGTGATGGGTCACATGGGCC TTTGGGGAATCTGTGTGGAAGATTTAAGAAATCACACAATTAACAAGACAAACTGAAATCTACCCAAGAC TCAGTTAAATCAATGATGACAGTGAACTATGTGTCTGTTTGACACTTCAACTGTCATCGCACTAGTGACC AAAAGCAGAGCTGGTCCCCTCAGCACAGGACTGCACATTTTCTTTTGGGACTCAGAAGCTAAGAGGGAAT CCACATGAGCCATTGGGTAAACATGGCCATCCTTATGTGATGTTCAATAGCATGAGAGAGGTAGTCAAAG AATGGAGGAGGGAATGGAAAATAACTAATTTAATATGTGTTTGAGTTCATATTAAAATTAGGGCTGTATG ATCCTGGAAGGTGACACCATTGAGACTCAACCATTGTAGTCACCTTGCTGACCAACATTAACTGTCAGTC CAAGTGAACAGCCATCACTAAGTGTGTAGCCAGAGAAAATGACTCCCAGCCCCTGACTCTGGGGGACAAG GTACAGGTGTGTCACACAGGATCCCTGGAGTGTGCTGTGGACCCCAGTGCAGCACTCACTGGAACACAGT GGGCTCACAGGGTGTGTGGTCTTCCCTCTCACTCAGCCCTGGATTGCAGAAGACCCTGCCTCTCAGAGTC CAAGTCCTGATTTACTAGCGGGAGGGAGCACTGTAACTTGTGGGGCAGAGGAAGAGCAGGAGGCTTCTGC ATGTTTAGTTCTGACAGGAAACAGTCTAAGTTCACAGGAATCTCTTCTTTAATTCGAACCCTGATTTAAC TTGTCTAATTATCCAGGTTAGCAGCCTTCCCTGGCACCTGTGGAGACTGAAACAAATGATAATAGGAGGG CATGGAGAGACTGGATCACTCCAATGCAGACAAAAGCTGACTAGAAACAAAATAAGCCCCCCCCCCCACC AAAAAAAAAGTTAAGAAAAAAGAAAGGCCAGGAGTGGTGGCACATGCCTTTAATCCCAGCACTCGGGAGC CAGAGGCAGGCAGATTTCTGAGTTCAAGGCCAACCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCT ATACAGAGAAACCCCGTCTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGGAAAGAGA GAGAGAGAGAGAGAGAGAAGAAAATCCACGATTTATATTCCTCCAGGGTAGCCCATAAGGGCTCATGTGA CCATTCACCACTGTAGGAATAGATAGAATTGGGTTGTTAAGGGGTGCAAAACCCCACAGACAGGGAGGGA TCTGTGAGAATGTATTCTTTGGAAAATTCTAGAAACTAGGTGAACCGTCCTAGTGTAGAGGAGTCCATGT CTCTTATCAGGTAAGGAGACTTCCGGTCCTGAGGTGTAAGGGCTGACACATTCAGCAGGACAGGAGTCAG TGTCTACAGACAGTGGGCGTGGGCAGAGAACCCGGCCTGGGAGAGGCCATGGCTGACAGAGGCTGCAGGG GAACTGAAGGGAGCAGCTGTTGTTACCTCAGGGAAATTCTCTCCTTCCCTCCTGAGACAGCCTGGGCACT GTCCAGGGAGAAGGCTGAGCCCTGGGAGAGTCAGTGCAGTCACTGTGATGAGGGATCAGGAGACCAGCGA CACTCTCTCCCCTCTGAGACAGGGGCATCACCCCAGCTGAGGGTTTCTTCTTCCCCAGGACTGAGCCCCA GCCCGAGGGCAGAGGGAGGAGCTGCCCGCGGCCCCTGCACCTGTGCGCAGCAGCGTCGCGTTCCCGTTCT TCAGGTATCTGCGGAGCCACTCCACGCACGTGCCCTCCAGGTAGGCCCTGAGTCTCTCTGCTTCACCAGC CTGCTCCCACTTGTGTTTGGTGATCAGCGCCGCCATGTCCGCCGCCGTCCACGTTTTCAGGTCTTCGTTC AGGGCGATGTAATCGCAGCCGTCGTAGGCGTACTGCTGGTACCCGCGGAGGAGTCGCCCGTCGGACCCCA CTTCACAGCCAGAGATCACCTGAATAGTGTGAGAGCCTGCGGGACCCCGCGGTCAGCCCCGCTCACCCGC CCCGGCCCCGCCCGCCCGCGGACTCCTCCAAACTGAAAGAGAAACCGGTTCCGGGTCCGTTCTGTTCCCG AACCTCGGACTTGGGACCCGGGACGTCAGCGTCCCTGTGTCGGGACGTGGAGGGGTCGTGACCTCCGACC CGGGGTCACTCACCGCCCTTGCTCTGGTTGTAGTAGCCGAGCAGGGTCCTCAGGTCCACTCGGAAACTCT GCTCATTGCCCTTGGCTTTCTGTGTCTCCCGCTCCCAATACTCGGGCCCCTCCTGCTCCATCCACCGCGC CCGCGGCTCATATCTCGGATTCTCCGCGTCGCTGTCGAAGCGCACGAACTCCGTGTCGTCCACGTAGCCG ACTTCCATGTACCGGGGCTCCCCGAGGCCGGGCCGGGACACGGCGGTGACGAAATACCTCAGCGAGTGTG GGCCTGGGGGCGGCGCGCGGTGAGACCCGGACCACCTCACCAGACCCCGGGCGGCTGCGCGGGCTGAGAG GGGAGCAGGGCGCGGGACTCGGGTGGAGAAGGGGCGGAGGGTCCGGTGGGCGACCCGAGGACGCGGCTTC CCCGGTGCCGCCCCTCCCCTCAGAGGCCGTTTCCCTCCCGGCCCGGTACTCACCCGCGCGGGTCTGAGTC GGAGCCAGGGCGGCCGCCAACAGCAGGAGCAGCGTGCACGGTACCATCGCACCTGTCGGCGATTCGCGAC TTCTGAGTTCTGCGGGCTGCGTGGACTTTATATCCAGCGTCCGCGGCGACACTGATTGGT
Protein Sequence	>NP_001001892.2 H-2 class I histocompatibility antigen, K-W28 alpha chain isoform 1 precursor [Mus musculus] MVPCTLLLLLAAALAPTQTRAGPHSLRYFVTAVSRPGLGEPRYMEVGYVDDTEFVRFDSDAENPRYEPRA RWMEQEGPEYWERETQKAKGNEQSFRVDLRTLLGYYNQSKGGSHTIQVISGCEVGSDGRLLRGYQQYAYD GCDYIALNEDLKTWTAADMAALITKHKWEQAGEAERLRAYLEGTCVEWLRRYLKNGNATLLRTDSPKAHV THHSRPEDKVTLRCWALGFYPADITLTWQLNGEELIQDMELVETRPAGDGTFQKWASVVVPLGKEQYYTC HVYHQGLPEPLTLRWEPPPSTVSNMATVAVLVVLGAAIVTGAVVAFVMKMRRRNTGGKGGDYALAPGSQT SDLSLPDCKVMVHDPHSLA
Molecule Role	Protective antigen
Molecule Role Annotation	A mouse H-2Kb (H2-K1) gene was introduced into two poorly immunogenic tumor cell lines, a mouse colonic carcinoma cell line, MCA-26 (H-2Kd), and a rat mammalian carcinoma cell line, LN-4, in an effort to stimulate tumor rejection. Results showed that the expression of xenogeneic MHC class I antigen completely abolished the LN-4 tumorigenicity in rats, whereas the expression of allogeneic MHC class I antigen only partially reduced the MCA-26 tumorigenicity in mice. The immunized rats that experienced LN-4/H-2Kb tumor regression further developed protective immunity against a subsequent challenge of LN-4 cells. Adenovirus-mediated H-2Kb gene transfer in vivo can further significantly inhibit pre-established LN-4 tumors (Campbell et al., 2000).
Related Vaccines(s)	Cancer recombinant vector vaccine encoding H-2Kb , Poliovirus vector expressing model antigen H2-Kb-restricted CTL epitope
References

H2-K1