VIOLIN: Vaccine Investigation and Online Information Network

Spa32
Gene Name	Spa32
Sequence Strain (Species/Organism)	Shigella flexneri 5a str. M90T
VO ID	VO_0010992
NCBI Gene ID	876502
NCBI Protein GI	13449098
Locus Tag	pWR501_0160
Genbank Accession	AF348706
Protein Accession	NP_085314
Taxonomy ID	1086030
Plasmid No	pWR501
Gene Starting Position	123961
Gene Ending Position	124839
Gene Strand (Orientation)	+
Protein Name	invasion protein
Protein pI	5.89
Protein Weight	31884.85
Protein Length	292
Protein Note	residues 1 to 292 of 292 are 100.00 pct identical to residues 1 to 292 of 292 of product encoded by GenBank Accession Number D50601 ORF20 [Shigella sonnei]
DNA Sequence	>NC_002698.1:123961-124839 Shigella flexneri 5a virulence plasmid pWR501, complete sequence TATGGCATTAGATAATATAAACCTAAATTTTAGTAGTGACAAACAGATAGAAAAATGTGAGAAACTATCT TCAATAGATAATATTGATAGTCTCGTTTTGAAGAAGAAGAGGAAGGTTGAAATTCCTGAGTACTCTTTAA TTGCATCTAACTATTTTACTATTGATAAGCACTTTGAACATAAGCATGATAAAGGAGAAATTTATAGTGG CATTAAAAATGCGTTCGAACTTAGAAACGAACGAGCGACATATTCTGATATTCCAGAATCAATGGCCATT AAAGAAAATATTTTGATACCAGATCAAGATATCAAAGCAAGGGAAAAAATAAATATCGGCGATATGAGGG GGATCTTTTCATATAATAAGAGTGGAAATGCAGACAAGAACTTCGAAAGAAGTCATACTTCTTCTGTAAA CCCTGATAATCTGCTAGAATCTGATAATAGAAATGGTCAAATTGGTTTAAAAAATCATAGCTTGTCTATT GATAAGAATATTGCTGACATCATTTCTTTACTAAATGGAAGTGTTGCTAAATCATTTGAGCTGCCTGTAA TGAATAAAAATACTGCAGACATAACCCCATCCATGTCATTGCAAGAAAAATCAATAGTTGAAAATGATAA AAATGTTTTTCAAAAAAATAGTGAAATGACTTACCACTTTAAACAGTGGGGGGCTGGACATTCTGTTAGT ATTTCAGTGGAGTCTGGTTCTTTTGTTCTAAAACCGTCAGATCAATTTGTAGGAAATAAACTTGACTTAA TTTTGAAACAAGATGCTGAGGGTAATTACAGATTTGATAGCAGTCAACATAATAAGGGGAATAAAAATAA TAGTACAGGATATAATGAACAGAGTGAAGAAGAATGCTA
Protein Sequence	>NP_085314.1 invasion protein (plasmid) [Shigella flexneri 5a str. M90T] MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEYSLIASNYFTIDKHFEHKHDKGEIYSG IKNAFELRNERATYSDIPESMAIKENILIPDQDIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVN PDNLLESDNRNGQIGLKNHSLSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDK NVFQKNSEMTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKGNKNN STGYNEQSEEEC
Molecule Role	Protective antigen
Molecule Role Annotation	S. flexneri M90T is a colonisation-defective mutant with a transposon in fnr, which encodes a regulator of anaerobic metabolism. To establish whether FNR-mediated repression of spa32 and spa33 contributes to increased Shigella entry into cells in an anaerobic cabinet, recombinant M90T strains were constructed which contain spa32 and spa33 under the control of either their native promoters (M90Tp32/33) or promoters with disrupted FNR boxes (M90Tp#32/#33).The presence in M90T of spa32 and spa33 alleles that are not repressed by FNR led to IpaB secretion during anaerobic growth, and loss of the increased entry of Shigella into cells in an anaerobic cabinet (Marteyn et al., 2010).
References

Spa32