VIOLIN: Vaccine Investigation and Online Information Network

PhoP
Gene Name	PhoP
Sequence Strain (Species/Organism)	Mycobacterium tuberculosis H37Rv
NCBI Gene ID	888772
NCBI Protein GI	15607897
Locus Tag	Rv0757
Genbank Accession	BX842574
Protein Accession	NP_215271
3D structure: PDB ID	3R0J
Taxonomy ID	83332
Gene Starting Position	851607
Gene Ending Position	852350
Gene Strand (Orientation)	?
Protein Name	two component system response transcriptional positive regulator PHOP
Protein pI	6.31
Protein Weight	24835.35
Protein Length	247
Protein Note	Rv0757, (MTCY369.02), len: 247 aa. Possible phoP, two component system response phosphate regulon transcriptional regulator (see citations below), highly similar to various transcriptional regulators e.g. CAC32360.1\|AL583945 putative two component system response regulator from Streptomyces coelicolor (271 aa); T45446 probable two-component response regulator from Mycobacterium leprae (253 aa); and similar to phoP proteins e.g. P13792\|PHOP_BACSU alkaline phosphatase synthesis transcription regulatory protein from Bacillus subtilis (240 aa), FASTA scores: opt: 594, E(): 2.3e-33, (41.0% identity in 234 aa overlap); etc. Also highly similar to Rv3765c from Mycobacterium tuberculosis (234 aa), Rv1033c (257 aa), RV0903c\|MTCY31.31c\|Q10531 (236 aa), FASTA score: (45.4% identity in 229 aa overlap); MTCY10G2_16 and MTU88959_1.
DNA Sequence	>gi\|57116681:851607-852350 Mycobacterium tuberculosis H37Rv, complete genome AATGCGGAAAGGGGTTGATCTCGTGACGGCGGGAACCCCAGGCGAAAACACCACACCGGAGGCTCGTGTC CTCGTGGTCGATGATGAGGCCAACATCGTTGAACTGCTGTCGGTGAGCCTCAAGTTCCAGGGCTTTGAAG TCTACACCGCGACCAACGGGGCACAGGCGCTGGATCGGGCCCGGGAAACCCGGCCGGACGCGGTGATCCT CGATGTGATGATGCCCGGGATGGACGGCTTTGGGGTGCTGCGCCGGCTGCGCGCCGACGGCATCGATGCC CCGGCGTTGTTCCTGACGGCCCGTGACTCGCTACAGGACAAGATCGCGGGTCTGACCCTGGGTGGTGACG ACTATGTGACAAAGCCCTTCAGTTTGGAGGAGGTCGTGGCCAGGCTGCGGGTCATCCTGCGACGCGCGGG CAAGGGCAACAAGGAACCACGTAATGTTCGACTGACGTTCGCCGATATCGAGCTCGACGAGGAGACCCAC GAAGTGTGGAAGGCGGGCCAACCGGTGTCGCTGTCGCCCACCGAATTCACCCTGCTGCGCTATTTCGTGA TCAACGCGGGCACCGTGCTGAGCAAGCCTAAGATTCTCGACCACGTTTGGCGCTACGACTTCGGTGGTGA TGTCAACGTCGTCGAGTCCTACGTGTCGTATCTGCGCCGCAAGATCGACACTGGGGAGAAGCGGCTGCTG CACACGCTGCGCGGGGTGGGCTACGTACTGCGGGAGCCTCGATG
Protein Sequence	>gi\|15607897\|ref\|NP_215271.1\| two component system response transcriptional positive regulator PHOP [Mycobacterium tuberculosis H37Rv] MRKGVDLVTAGTPGENTTPEARVLVVDDEANIVELLSVSLKFQGFEVYTATNGAQALDRARETRPDAVIL DVMMPGMDGFGVLRRLRADGIDAPALFLTARDSLQDKIAGLTLGGDDYVTKPFSLEEVVARLRVILRRAG KGNKEPRNVRLTFADIELDEETHEVWKAGQPVSLSPTEFTLLRYFVINAGTVLSKPKILDHVWRYDFGGD VNVVESYVSYLRRKIDTGEKRLLHTLRGVGYVLREPR
Molecule Role	Virmugen
Molecule Role Annotation	Study evaluated immunogenicity and protection against Mycobacterium tuberculosis in rhesus monkeys with an attenuated M. tuberculosis with a disrupted phoP gene (SO2) as a single-dose vaccine. SO2 treatment showed reduced average lung bacterial counts (1.0 and 1.2 log respectively, compared with 0.4 log for BCG) and significant protective effect by reduction in C-reactive protein levels, body weight loss, and decrease of erythrocyte-associated hematologic parameters (MCV, MCH, Hb, Ht) as markers of inflammatory infection, all relative to non-vaccinated controls (Verreck et al., 2009).
Related Vaccines(s)	M. tuberculosis phoP mutant SO2
References

PhoP