VIOLIN: Vaccine Investigation and Online Information Network

Ag85B from M. tuberculosis H37Rv

General Information

Protegen ID

118

Sequence Strain (Species/Organism)

Mycobacterium tuberculosis H37Rv

VO ID

VO_0012364

Taxonomy ID

83332

Other Database IDs

EMBL: AE000516
EMBL: AF198032
EMBL: BX842578
EMBL: U38939
EMBL: X62398
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
InterPro: IPR000801
KEGG: mtc:MT1934
KEGG: mtu:Rv1886c
PDB: 1F0N
PDB: 1F0P
Pfam: PF00756
PIR: C70516
TIGR: MT1934
TubercuList: Rv1886c
UniProt: P31952

Molecule Role

Protective antigen

Molecule Role Annotation

In mice, a primary vaccination with Ag85B-encoding plasmid DNA (DNA-85B) was protective against Mycobacterium tuberculosis (MTB) infection and associated with Ag85B-specific CD4+ T cells producing IFN-gamma and controlling intramacrophagic MTB growth (Palma et al., 2007).

COG

COG0627R, under R: General function prediction only

Related Vaccines(s)

AER in PLGA nanoparticles + CpG + MPLA , Ag85B and ESAT-6 fusion protein , Ag85B:HspX:hFcγ1 , AR2+DMC , Arabinomannan-tetanus toxoid conjugate , ARM , ERA005f , H1 / CAF01 , H4-IC31 , H56:IC31 , LT-BCG- Ag85B /Rv3425 , M. tuberculosis DNA Vaccine encoding Ag85B Protein , M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83 , MVA/IL-15/5Mtb vaccine , rBCG-Ag85A/ Ag85B , Spore-FP1

References

Palma et al., 2007: Palma C, Iona E, Giannoni F, Pardini M, Brunori L, Orefici G, Fattorini L, Cassone A. The Ag85B protein of Mycobacterium tuberculosis may turn a protective immune response induced by Ag85B-DNA vaccine into a potent but non-protective Th1 immune response in mice. Cellular microbiology. 2007; 9(6); 1455-1465. [PubMed: 17250590].

Gene Information

Gene Name

Ag85B from M. tuberculosis H37Rv

NCBI Gene ID

885785

Genbank Accession

AL123456

Locus Tag

Rv1886c

Gene Starting Position

2134889

Gene Ending Position

2135866

DNA Sequence

>NC_000962.3:2134889-2135866 Mycobacterium tuberculosis H37Rv, complete genome
GTCAGCCGGCGCCTAACGAACTCTGCAGGTCACCCTTCATGGCGTTGAGCTGAGCGCCCCAGTACTCCCA
GCTGTGCGTGCCGTTGGGCGGGAAGTTGAACACGGCGTTGTGCCCGCCCGCGGCGTTGTACGCATCCTGG
AACTTCAGGTTGCTGCTACGAACGAAGTTCTCCAAGAACTCGGCGGGTATGTTGGCACCGCCCAACTCGT
TCGGGGTGCCGTTCCCGCAATAAACCCATAGCCGGGTGTTGTTTGCGACCAGCTTGGGGATCTGCTGCGT
AGGGTCGTTGCGCTCCCATGCCGGGTCACTCGAGGGACCCCACATGTCTGCGGCCTTGTAACCGCCGGCG
TCACCCATCGCGAGGCCGATCAGGCTAGGCCCCATCCCCTGAGAGGGGTCCAGCAGGGCCGACAGCGAGC
CGGCGTAGATGAACTGCTGGGGGTGGTAGGCGGCCAAGATCATTGCCGACGAGCCGGCCATCGACAAGCC
GATTGCAGCGCTGCCGGTGGGCTTCACGGCCCTGTTGGCGGACAACCATTGCGGCAGCTCGCTGGTCAGG
AAGGTTTCCCACTTGTAAGTCTGGCAGCCAGCCTTACCGCAGGCCGGGCTGTACCAGTCGCTGTAGAAGC
TGGACTGCCCGCCGACCGGCATGACTATCGACAGTCCCGACTGGTAGTACCACTCGAACGCCGGGGTGTT
GATATCCCAGCCGTTGTAGTCGTCTTGGGCGCGCAGGCCGTCGAGCAGATAAACCGCAGGTGAGTTGTTC
CCACCGCTCTGGAACTGAACCTTGATGTCGCGGCCCATCGACGGCGACGGCACCTGCAGGTACTCGACCG
GCAGCCCCGGCCGGGAGAACGCGCCCGCGGTTGCCGCTCCGCCGGCAAGCCCCACCAGGCCCGGAAGGAC
TACAGCCGCTGCCGTGCCGATCATCAATCGGCGTCCCCAAGCTCGAATCTTTCGGCTCACGTCTGTCA

Protein Information

Protein Name

diacylglycerol acyltransferase/mycolyltransferase Ag85B

NCBI Protein GI

15609023

Protein Accession

NP_216402

Protein pI

5.72

Protein Weight

33091.46

Protein Length

325

Protein Note

Rv1886c, (MT1934, MTCY180.32), len: 325 aa. fbpB (alternate gene names: mpt59, 85B), precursor of the 85-B antigen (fibronectin-binding protein B) (mycolyl transferase 85B) (EC 2.3.1.-) (see citations below), highly similar to other Mycobacterial antigen precursors e.g. P12942|A85B_MYCBO ANTIGEN 85-B PRECURSOR from Mycobacterium bovis (323 aa); P21160|A85B_MYCKA ANTIGEN 85-B PRECURSOR from Mycobacterium kansasii (325 aa); etc. Also highly similar to Mycobacterium tuberculosis antigen precursors: Rv3804c|fbpA (338 aa), Rv0129c|fbpC2 (340 aa), and Rv3803c|fbpC1 (299 aa). TBparse score is 0.912.; mpt59; 85B

Protein Annotation

FUNCTION: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity (UniProt: P31952)

SUBCELLULAR LOCATION: Secreted protein (UniProt: P31952)

SIMILARITY: Belongs to the mycobacterial A85 antigen family (UniProt: P31952)

Protein Sequence

>NP_216402.1 diacylglycerol acyltransferase/mycolyltransferase Ag85B [Mycobacterium tuberculosis H37Rv]
MTDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQSGG
NNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETF
LTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGD
AGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKF
QDAYNAAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGAG

Epitope Information

IEDB Linear Epitope