VIOLIN: Vaccine Investigation and Online Information Network

Brucella spp.

Table of Contents

Protective Antigens
1. AsnC
2. B. abortus strain 19 L7/L12
3. B. melitensis M5 P39
4. BAB1_0278
5. BCSP31
6. Bfr from B. abortus S19
7. Bfr from B. melitensis biovar Abortus 2308
8. Bp26
9. cobB
10. DnaK
11. Gap
12. IalB
13. L7/L12
14. Omp16
15. omp16
16. Omp19
17. Omp25
18. Omp31
19. omp31
20. ORF
21. P39 from Brucella abortus
22. PALFn
23. RplL
24. SodC from B. abortus strain 2308
25. SurA
26. Tig

I. Vaccine Related Protective Antigens

1. AsnC

Gene Name : AsnC
Sequence Strain (Species/Organism) : Brucella abortus 2308
NCBI Gene ID : 3788213
NCBI Protein GI : 489054600
Locus Tag : BAB_RS24000
Genbank Accession : JMRZ01000003
Protein Accession : WP_002964767
Taxonomy ID : 359391
Chromosome No : I
Gene Starting Position : 1633929
Gene Ending Position : 1634399
Gene Strand (Orientation) : -
Protein Name : ArsR family transcriptional regulator
Protein pI : 5.32
Protein Weight : 15857.78
Protein Length : 156

DNA Sequence : Show Sequence

>NC_007618.1:1633929-1634399 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence, strain 2308
TCTAACGCCCATCCAGCGGGACGGCTGTGGTGTCCTTGATCTCTTCCATCACGAAAGAGGCCGATACATC
CGATAGCGGTACACAGGCTATGAGGCGCTGGTAGAGCCGGTCATAAGCTCTGACATCGGAAATGCGTGCC
TTCAGCACATAGTCCAGATCGCCGGTCATGCGATAGGCGCCGACGATTTCCGGGAAGCGAGCCACGGCAG
CGCGGAATTTGTTCAGCCATTCGGGATCATGGGCGGAGGTGCGCACCAGAATGAAAACCGAGAGGCCATA
GCCGACCATTTCCGCATCCACCAGCGCCACGCGACCTTTGATGACGCCATCGTCTTCCATGCGCTTTACG
CGCCGCCAGCAGGCATTGCGCGAAAGATTCACCCGTTCGGAAAGGGAATCAACCGACAAAGTGCCGTCGT
TTTGCAACTCAGCCAGGATTTTTCGGTCTATATCGTCCATAGATGCTGTCA

Protein Sequence : Show Sequence

>WP_002964767.1 MULTISPECIES: Lrp/AsnC family transcriptional regulator [Brucella]
MTASMDDIDRKILAELQNDGTLSVDSLSERVNLSRNACWRRVKRMEDDGVIKGRVALVDAEMVGYGLSVF
ILVRTSAHDPEWLNKFRAAVARFPEIVGAYRMTGDLDYVLKARISDVRAYDRLYQRLIACVPLSDVSASF
VMEEIKDTTAVPLDGR

Molecule Role : Protective antigen
Molecule Role Annotation : Mice given BAB1_1688 (AsnC) plus adjuvant could provide protection against virulent B. abortus infection, similarly with the known protective antigen Cu-Zn SOD and the license vaccine S19 (Fu et al., 2012).
Related Vaccine(s): Recombinant B. abortus AsnC protein with CFA/IFA adjuvant

2. B. abortus strain 19 L7/L12

Gene Name : B. abortus strain 19 L7/L12
Sequence Strain (Species/Organism) : Strain 19
VO ID : VO_0010928
NCBI Protein GI : 304271
Genbank Accession : L19101
Other Database IDs : CDD:234671
CDD:100102
Taxonomy ID : 235
Gene Strand (Orientation) : ?
Protein Name : ribosomal protein L7/L12
Protein pI : 4.49
Protein Weight : 11795.56
Protein Length : 183
Protein Note : biovar Abortus

DNA Sequence : Show Sequence

>gi|304270|gb|L19101.1|BRURPL712X Brucella abortus ribosomal protein L7/L12 gene, complete cds 
CAAGCTCGTTATTCTCGGTGGCGCAATGGGTGCAACGACGCTTAGACCGATGGCGTCAAGTCGCTTCGTT
CGCTCCCGTCGCTCGACGAACTGCGCGCAAAGCTGGTTGGTATGATCCAGACCCCGGCTCAGCGTCTTGC
AGTGCTTACCAGCGCTCCGCGGGCCAGATCGCCCGCGTTATTGCGCGCACGCCCGGAAGAACGAGGCGGC
TTAAGGCCGTTTCTCGCTGTCAACAGTTCAAACCTTAATTATAGGAAATACAAAAATGGCTGATCTCGCA
AAGATCGTTGAAGACCTTTCGGCCCTGACCGTTCTGGAAGCCGCTGAGCTGTCCAAGCTTCTCGAAGAGA
AGTGGGGCGTTTCGGCTGCTGCTCCGGTCGCTGTTGCTGCTGCCGGTGGCGCTGCCCCTGCTGCTGCCGC
AGAAGAAAAGACCGAATTCGACGTCGTTCTCGCTGACGGCGGCGCTAACAAGATCAACGTGATCAAGGAA
GTGCGCGCACTCACCGGTCTCGGCCTCAAGGAAGCCAAGGACCTGGTCGAAGGCGCTCCGAAGGCTGTCA
AGGAAGGCGCCTCGAAGGACGAAGCTGAGAAGATCAAGGCACAGCTCGAAGCTGCTGGCGCCAAGGTTGA
ACTCAAGTAAGTTTGGACTATT

Protein Sequence : Show Sequence

>AAA19863.1 ribosomal protein L7/L12 [Brucella abortus]
MADLAKIVEDLSALTVLEAAELSKLLEEKWGVSAAAPVAVAAAGGAAPAAAAEEKTEFDVVLADGGANKI
NVIKEVRALTGLGLKEAKDLVEGAPKAVKEGASKDEAEKIKAQLEAAGAKVELK

Molecule Role : Protective antigen
Related Vaccine(s): B. abortus DNA vaccine expressing BCSP31, SOD and L7/L12 , B. abortus DNA vaccine pcDNA3.1-p39-L7/L12 encoding p39-L7/L12 fusion protein , B. abortus subunit vaccine using L7/L12

3. B. melitensis M5 P39

Gene Name : B. melitensis M5 P39
Sequence Strain (Species/Organism) : B. melitensis strain M5
VO ID : VO_0010948
NCBI Protein GI : 122892474
Genbank Accession : EF189139
Protein Accession : ABM67295
Other Database IDs : CDD:224567
CDD:304360
Taxonomy ID : 1004957
Gene Strand (Orientation) : ?
Protein Name : immunogenic 39-kDa protein
Protein pI : 4.69
Protein Weight : 40682.69
Protein Length : 483
Protein Note : biovar: 1 str. M5

DNA Sequence : Show Sequence

>gi|122892473|gb|EF189139.1| Brucella melitensis strain M5 immunogenic 39-kDa protein gene, complete cds
ATGGGTACGGCTTCCTGTGGTTTGCTCGCCGGAATGGCGCCTGTTGCCAATGCGCAGGAAAAGCAGAATG
TCGAGGTTCTGCACTGGTGGACGTCCGGCGGCGAAGCGTCCGCGCTTGAAGTTTTGAAAAAAGATCTTGA
AAGCAAGGGCATTAGCTGGACCGATATGCCGGTTGCAGGTGGCGGCGGCACGGAAGCCATGACCGTTTTG
CGCGCGCGCGTTACCGCAGGCAATGCGCCAACCGCGGTGCAGATGCTGGGTTTTGACATTCGCGACTGGG
CGGAGCAGGGCGCACTCGGCAATCTCGATACGGTTGCTTCCAAGGAAGGCTGGGAAAAGGTTATTCCGGC
TCCCTTGCAGGAATTTGCCAAATATGACGGCCACTGGATTGCTGCGCCCGTCAATATTCACTCCACCAAC
TGGATGTGGATCAACAAGGCTGCTCTCGACAAGGCTGGCGGCAAGGAGCCGACCAATTGGGATGAGCTGA
TTGCGCTTCTCGACAATTTCAAGGCGCAGGGCATTACGCCGATCGCGCATGGCGGCCAGCCGTGGCAGGA
TGCAACCATTTTCGATGCGGTTGTTCTTTCATTCGGCCCGGATTTCTACAAGAAGGCCTTCATCGATCTC
GACCCGGAAGCACTGGGCAGCGATACCATGAAGCAGGCCTTCGACCGCATGTCCAAGCTTCGCACCTATG
TTGATGACAACTTCTCCGGCCGTGACTGGAACCTTGCTTCGGCCATGGTTATCGAAGGCAAGGCCGGTGT
CCAGTTCATGGGCGACTGGGCGAAGGGCGAGTTCCTCAAGGCGGGCAAGAAGCCGGGTGAGGATTTCGTC
TGCATGCGTTATCCGGGCACGCAGGGTGCTGTCACTTTCAATTCCGGCATGTTCGCCATGTTCAAGGTTT
CGGAAGACAAGGTTCCCGCACAGCTTGAAATGGCTTCGGCGATTGAAAGCCCTGCCTTCCAGTCTGCCTT
TAATGTGGAGAAGGGGTCGGCCCCGGCACGCACGGATGTGCCCGATACCGCTTTCGATGCCTGTGGCAAG
AAGACCATTGCCGATGTCAAGGAAGCAAACAGCAAGGGCACTCTGCTTGGCTCCATGGCGCATGGCTATG
CCAATCCGGCTGCCGTGAAGAATGCGATCTACGACGTCGTGACCCGCCAGTTCAACGGCCAGCTTTCTTC
GGAAGATGCCGTCAAGGAACTCGTTGTGGCGGTTGAAGCCGCAAAATAAGGCCACGCGGAAACGTCCGGG
GGAACCTCCCGGACGTTTTTCCTGCGTGCTCCAGCAGAAGCGCATAGGCGGTTGGCGCAGCTAACCGGCA
CTTACGACGGAATTGCATGCCGATATGCCCGCCTCACCATCCGAGGCGCGTTTCGTGGGACATAGAGC

Protein Sequence : Show Sequence

>ABM67295.1 immunogenic 39-kDa protein [Brucella melitensis bv. 1 str. M5]
MAPVANAQEKQNVEVLHWWTSGGEASALEVLKKDLESKGISWTDMPVAGGGGTEAMTVLRARVTAGNAPT
AVQMLGFDIRDWAEQGALGNLDTVASKEGWEKVIPAPLQEFAKYDGHWIAAPVNIHSTNWMWINKAALDK
AGGKEPTNWDELIALLDNFKAQGITPIAHGGQPWQDATIFDAVVLSFGPDFYKKAFIDLDPEALGSDTMK
QAFDRMSKLRTYVDDNFSGRDWNLASAMVIEGKAGVQFMGDWAKGEFLKAGKKPGEDFVCMRYPGTQGAV
TFNSGMFAMFKVSEDKVPAQLEMASAIESPAFQSAFNVEKGSAPARTDVPDTAFDACGKKTIADVKEANS
KGTLLGSMAHGYANPAAVKNAIYDVVTRQFNGQLSSEDAVKELVVAVEAAK

Molecule Role : Protective antigen
Molecule Role Annotation : The 39-kDa protein is immunogenic.
Related Vaccine(s): B. melitensis P39 protein vaccine

4. BAB1_0278

Gene Name : BAB1_0278
Sequence Strain (Species/Organism) : Brucella melitensis biovar Abortus 2308
NCBI Protein GI : 82615279
Other Database IDs : CDD:264424
EnsemblGenomes-Gn:BAB1_0278
EnsemblGenomes-Tr:CAJ10234
InterPro: IPR011681
UniProtKB/TrEMBL: Q2YPC5
Taxonomy ID : 359391
Gene Strand (Orientation) : ?
Protein Name : conserved hypothetical protein
Protein pI : 8.51
Protein Weight : 8787.28
Protein Length : 148
Protein Note : GcrA cell cycle regulator; cl11564

Protein Sequence : Show Sequence

>CAJ10234.1 conserved hypothetical protein [Brucella abortus 2308]
MHWKSRSPLSEALPMFDKLTSPHEGFRLADLSRRQCKWPVNRAQAGELHLFCGEAVQNGHPYCEEHCGKA
YTGKAGSR

Molecule Role : Protective antigen
Related Vaccine(s): B. abortus DNA vaccine pVF278

5. BCSP31

Gene Name : BCSP31
Sequence Strain (Species/Organism) : Brucella abortus
NCBI Nucleotide GI : 144104
NCBI Protein GI : 144105
Protein Accession : AAA22993.1
Other Database IDs : CDD:273982
Taxonomy ID : 235
Gene Strand (Orientation) : ?
Protein Name : cell surface protein
Protein pI : 7.77
Protein Weight : 32204.04
Protein Length : 397
Protein Note : biovar Abortus

DNA Sequence : Show Sequence

>gi|144104|gb|M20404.1|BRUBCSP B.abortus BCSP31 gene encoding a 31-KDa cell surface protein, complete cds
GCGCCTATACGCGCGACCTGATCCAGTCTTTTGCATCGCGGTGCCATGTGCGTCTGGTGGGGGCCGACGG
GCTGGCGGCGATTGCCGAAGCGCATATTCGCGGTGAAAGTTTCGATGAGGCGCTGGTCATGGCGCAGATC
GCGCCATGTTTTATCGAAAAGGATGGCAAGCGCACCGATATCGTGGTGCTTGCCTGTACGCATTATCCGT
TTCTCGTCAATGTATTGCGCCGTCTGGCTCCATGGCCGGTGGATTGGCTGGACCCGGCGGAAGCCATTGC
ACGACGCATGAAATCGCTTTTGCCTGCGCGAAGTGACGATGATGAATTTCATTCTCAAGATGACCTAGCA
TTCTTCACATCCAGGAAACCCGACTATGCCATTCGCCGCCTGATGCAGGGTTTTGGGCTGCGTTTTTAAT
CGTTTCAGTCGGCTCTGGCGGCGCTTGTCGGCAAGCGATTGTATTCTTTGGGAAAATCCAGAATAATGGA
ATGCGGTGGTTGACAATCGGCCTCAAGCTTCCTATGGTTTTCGGCATAATCTATGCGGGAAGAGGACTGG
TATTATGAAATTCGGAAGCAAAATCCGTCGCTTGGCTGTTGCGGCGGTGGCGGGCGCGATTGCGTTGGGA
GCGAGCTTTGCGGTTGCACAGGCCCCGACATTTTTCCGTATCGGCACTGGCGGCACAGCCGGAACCTATT
ATCCGATTGGTGGTCTGATCGCGAACGCGATTTCCGGCGCAGGCGAAAAGGGCGTGCCGGGTCTCGTCGC
GACGGCCGTTTCGTCGAATGGCTCGGTTGCCAATATCAATGCGATCAAGTCGGGCGCTCTGGAGTCCGGC
TTTACGCAGTCAGACGTTGCCTATTGGGCCTATAACGGCACCGGCCTTTATGATGGCAAGGGCAAGGTGG
AAGATTTGCGCCTTCTGGCGACGCTTTACCCGGAAACGATCCATATCGTTGCGCGTAAGGATGCAAACAT
CAAATCGGTCGCAGACCTGAAAGGCAAGCGCGTTTCGCTGGATGAGCCGGGTTCTGGCACCATCGTCGAT
GCGCGTATCGTTCTTGAAGCCTACGGCCTCACGGAAGACGATATCAAGGCTGAACACCTGAAGCCGGGAC
CGGCAGGCGAGAGGCTGAAAGATGGTGCGCTGGACGCCTATTTCTTTGTGGGCGGCTATCCGACGGGCGC
AATCTCGGAACTGGCCATCTCGAACGGTATTTCGCTCGTTCCGATCTCCGGGCCGGAAGCGGACAAGATT
CTGGAGAAATATTCCTTCTTCTCGAAGGATGTGGTTCCTGCCGGAGCCTATAAGGACGTGGCGGAAACAC
CGACCCTTGCCGTTGCCGCACAGTGGGTGACGAGCGCCAAGCAGCCGGACGACCTCATCTATAACATCAC
CAAGGTTCTCTGGAACGAGGATACACGCAAGGCACTCGATGCGGGCCATGCGAAGGGCAAGCTCATCAAG
CTCGATAGTGCGACGAGCAGCCTCGGTATTCCGCTGCATCCCGGCGCAGAACGCTTTTACAAGGAAGCGG
GCGTGCTGAAATAATCCCTCAATGATCGGTTCCTGATATCTTATTCCGAATTGAAGGGTGACATTGCGGC
AGCTCGTTATGCGCGCTGCTGCGCTCCCGTTTTCCAGAGCGGTTCCGGTTAGAACGGAATCGTTGGAACC
GCTCTATCTCTTTGTTTTTACGCATTATCCGACGCAAAACCGTTTCACGCTTTTGCTGGAAATGCTCTAG
CCTATTGAAATGCACGACCGGCAAAGTGGAGTTGGCCCGCATGACAGAAGAACAAAATGCAAAGCTT

Protein Sequence : Show Sequence

>AAA22993.1 cell surface protein precursor [Brucella abortus]
MKFGSKIRRLAVAAVAGAIALGASFAVAQAPTFFRIGTGGTAGTYYPIGGLIANAISGAGEKGVPGLVAT
AVSSNGSVANINAIKSGALESGFTQSDVAYWAYNGTGLYDGKGKVEDLRLLATLYPETIHIVARKDANIK
SVADLKGKRVSLDEPGSGTIVDARIVLEAYGLTEDDIKAEHLKPGPAGERLKDGALDAYFFVGGYPTGAI
SELAISNGISLVPISGPEADKILEKYSFFSKDVVPAGAYKDVAETPTLAVAAQWVTSAKQPDDLIYNITK
VLWNEDTRKALDAGHAKGKLIKLDSATSSLGIPLHPGAERFYKEAGVLK

Molecule Role : Protective antigen
Related Vaccine(s): B. abortus DNA vaccine expressing BCSP31, SOD and L7/L12

6. Bfr from B. abortus S19

Gene Name : Bfr from B. abortus S19
Sequence Strain (Species/Organism) : Brucella abortus S19
VO ID : VO_0010907
NCBI Gene ID : 6326162
NCBI Protein GI : 189022832
Locus Tag : BAbS19_II06320
Genbank Accession : CP000888
Protein Accession : YP_001932573
Taxonomy ID : 430066
Chromosome No : 2
Gene Starting Position : 673149
Gene Ending Position : 673634
Gene Strand (Orientation) : -
Protein Name : Bacterioferritin
Protein pI : 4.42
Protein Weight : 17971.98
Protein Length : 161

DNA Sequence : Show Sequence

>NC_010740.1:673149-673634 Brucella abortus S19 chromosome 2, complete sequence
CTTACTCAGCTTCGTCGGCGGGCGCCGCGTTAAGCTGGCCATAGCGTTCTCCGCCGATCTTGGCGAGAAG
GTCAAGCTGGGTTTCAAGGAAGTCGATATGGCCTTCTTCATCGGCCAGAAGTTCGTCGAAAAGCTGCTTC
GACACATAGTCGCCGAGCTTGTCGCAGATTTCGCGCGATTCCTTATACGAAGCGCGAGCGTCATATTCAC
CCTTGAGGTCAGCTTCGAGAACTTCCTTCACATTCTGGCCAATGCGCAACGGCGAAACGGTCTGGAGGTT
CGGAAAGCCTTCAAGGAAGATAATGCGATCAATCAGCTTGTCGGCGTGATGCATTTCCTCGATGGATTCC
TCGCGTTCCTTCTTTGCAAGGCGCGTGTAACCCCAATCGTTGAGAAGACGGTAGTGCAGCCAATACTGGT
TTACCGCACCGAGCTCAAGAAACAGTGCCTCGTTAAGCCGCTCGATGACCTTTGGTTCGCCTTTCA

Protein Sequence : Show Sequence

>YP_001932573.1 Bacterioferritin [Brucella abortus S19]
MKGEPKVIERLNEALFLELGAVNQYWLHYRLLNDWGYTRLAKKEREESIEEMHHADKLIDRIIFLEGFPN
LQTVSPLRIGQNVKEVLEADLKGEYDARASYKESREICDKLGDYVSKQLFDELLADEEGHIDFLETQLDL
LAKIGGERYGQLNAAPADEAE

Molecule Role : Protective antigen
Molecule Role Annotation : Following two intragastric immunizations in BALB/c mice, the Yersinia vectors harboring a DNA vaccine encoding BFR (P39) induced antigen-specific serum immunoglobulin and Th1-type responses among splenocytes. The best protection was conferred by a serotype O:9 strain carrying pCIP39, confirming the importance of the P39 T-cell antigen in this mechanism (Al-Mariri et al., 2002).
COG: COG2193P, under P: Inorganic ion transport and metabolism

7. Bfr from B. melitensis biovar Abortus 2308

Gene Name : Bfr from B. melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) : Brucella abortus 2308
VO ID : VO_0010968
NCBI Gene ID : 3828133
NCBI Protein GI : 489055925
Locus Tag : BAB_RS29555
Genbank Accession : JMRZ01000012
Protein Accession : WP_002966069
3D structure: PDB ID : 3FVB
Taxonomy ID : 359391
Chromosome No : II
Gene Starting Position : 673934
Gene Ending Position : 674419
Gene Strand (Orientation) : -
Protein Name : bacterioferritin
Protein pI : 4.42
Protein Weight : 17971.98
Protein Length : 161

DNA Sequence : Show Sequence

>NC_007624.1:673934-674419 Brucella melitensis biovar Abortus 2308 chromosome II, complete sequence, strain 2308
CTTACTCAGCTTCGTCGGCGGGCGCCGCGTTAAGCTGGCCATAGCGTTCTCCGCCGATCTTGGCGAGAAG
GTCAAGCTGGGTTTCAAGGAAGTCGATATGGCCTTCTTCATCGGCCAGAAGTTCGTCGAAAAGCTGCTTC
GACACATAGTCGCCGAGCTTGTCGCAGATTTCGCGCGATTCCTTATACGAAGCGCGAGCGTCATATTCAC
CCTTGAGGTCAGCTTCGAGAACTTCCTTCACATTCTGGCCAATGCGCAACGGCGAAACGGTCTGGAGGTT
CGGAAAGCCTTCAAGGAAGATAATGCGATCAATCAGCTTGTCGGCGTGATGCATTTCCTCGATGGATTCC
TCGCGTTCCTTCTTTGCAAGGCGCGTGTAACCCCAATCGTTGAGAAGACGGTAGTGCAGCCAATACTGGT
TTACCGCACCGAGCTCAAGAAACAGTGCCTCGTTAAGCCGCTCGATGACCTTTGGTTCGCCTTTCA

Protein Sequence : Show Sequence

>WP_002966069.1 MULTISPECIES: bacterioferritin [Brucella]
MKGEPKVIERLNEALFLELGAVNQYWLHYRLLNDWGYTRLAKKEREESIEEMHHADKLIDRIIFLEGFPN
LQTVSPLRIGQNVKEVLEADLKGEYDARASYKESREICDKLGDYVSKQLFDELLADEEGHIDFLETQLDL
LAKIGGERYGQLNAAPADEAE

Molecule Role : Protective antigen
Molecule Role Annotation : Brucella bacterioferritin is a protective antigen (Al-Mariri et al., 2001)
COG: COG2193P, under P: Inorganic ion transport and metabolism

8. Bp26

Gene Name : Bp26
Sequence Strain (Species/Organism) : Brucella melitensis bv. 1 str. 16M
VO ID : VO_0010962
NCBI Gene ID : 1196247
NCBI Protein GI : 17986819
Locus Tag : BMEI0536
Genbank Accession : AE008917
Protein Accession : NP_539453
Taxonomy ID : 224914
Chromosome No : I
Gene Starting Position : 557728
Gene Ending Position : 558480
Gene Strand (Orientation) : -
Protein Name : immunogenic protein
Protein pI : 6.97
Protein Weight : 24369.69
Protein Length : 250

DNA Sequence : Show Sequence

>NC_003317.1:557728-558480 Brucella melitensis bv. 1 str. 16M chromosome I, complete sequence
TTTACTTGATTTCAAAAACGACATTGACCGATACGTTATAGCTGTTTTCGCCTGCGGCAATCGGCACGGA
ATTGTCCGGTGCGGCTGCTAGCATGGTTCTGAACTGTCCGCGCGCAATTGGCATCGGCATGGGCGGGCGG
CTCAGTTCACTGATTTCCACCACACGGCCAAGCCCCACGCCTGCAGCGTCGGCAAGCGTCTTCGCCTTGG
CAATGGCATTGGCCACTGCGCGCTTGCGCGCCTCGTTGATCACGGCGGAGGGATTATCATTGACCAGGTT
CAAATCACCGCCCTGATTAACACCGAGCGTGACGGATTCATCCAAAATTTTTCCAACATTGGCCAGTTCG
CGCACGCGAACCGTGAGACTGGTGGATACAGAATAGCCGGTGATGGTAGGCTCTTTCAGGTTGTTCTTGT
CGTCAGGATAGACATAAATCGGCTGGATATTGATGCCGCCTGTCTGGAGATCGCGATCTTCGATGCCGGC
CTTCTTCATGGCATCGAGCACTTTTGTCATGGCTTCATTATTCGCGGTCATGGCTTCGCGCGCGGTCTTT
GCCTGGCGTAGCACCGAGAGATTGAGAATGGCCATATCGGGCGAGGCCGTCATCATGCCTTCCCCGGTGA
CGGCGATGCGCGCGGGCTGCGTCGTCATCTGATTCTCCTGTGCGAAAGCGGGCAGGCTGAAAGCGCCGAC
GAGCATGATTGTGGAAAATGAGGCTGCGAGAAAATTGCTAGCACGAGTGTTCA

Protein Sequence : Show Sequence

>NP_539453.1 immunogenic protein [Brucella melitensis bv. 1 str. 16M]
MNTRASNFLAASFSTIMLVGAFSLPAFAQENQMTTQPARIAVTGEGMMTASPDMAILNLSVLRQAKTARE
AMTANNEAMTKVLDAMKKAGIEDRDLQTGGINIQPIYVYPDDKNNLKEPTITGYSVSTSLTVRVRELANV
GKILDESVTLGVNQGGDLNLVNDNPSAVINEARKRAVANAIAKAKTLADAAGVGLGRVVEISELSRPPMP
MPIARGQFRTMLAAAPDNSVPIAAGENSYNVSVNVVFEIK

Molecule Role : Protective antigen
Molecule Role Annotation : This is a protective antigen (Yang et al., 2007).
COG: COG2968S, under S: Function unknown

9. cobB

Gene Name : cobB
Sequence Strain (Species/Organism) : Brucella abortus 2308
NCBI Gene ID : 3787893
NCBI Protein GI : 489054238
Locus Tag : BAB_RS22225
Genbank Accession : JMRZ01000005
Protein Accession : WP_002964414
Taxonomy ID : 359391
Chromosome No : I
Gene Starting Position : 1271743
Gene Ending Position : 1273053
Gene Strand (Orientation) : -
Protein Name : hydrogenobyrinate a,c-diamide synthase
Protein pI : 6.89
Protein Weight : 44156.43
Protein Length : 436
Protein Note : responsible for the amidation of carboxylic groups at position A and C of cobyrinic acid or hydrogenobrynic acid

DNA Sequence : Show Sequence

>NC_007618.1:1271743-1273053 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence, strain 2308
CTCACGCCGCTTCTCCGCTAAAATCAATGACATGCATGAAGGAGCCGGAGACGGAACCAACCCGCAAGCC
CGCTTCGCCAAGGTTCTCGCCCGATGCATCGCGCACCCGGAACAGGCGGTCGGCCTTTTCTTCCCGCACG
ATGCTTGCATAATGGAATTCATGCGCTTTCAAAGGCATGTCCCACGGCAATCCGCCAAGCGGCTCCAGCA
GGCGATAGCCAAGATGCAGTTTCCGCCTGGCAAAGCTCGTTTCCAATGGCAAAAGCCCCAGCATGGGATG
GTGAACGCCTGCCGCATCCTCCAGTGTTTTCCCGAGGACCATATAGCCGCCGCACTCGCCATAGACCGTG
ACGCCACGCGCGGCAGCGTCCCCTATCGCCGTGCGGAAACGGGATGCACCGGCCAATCGTTGCGCATGAA
GTTCTGGATAACCACCGGGAAGATAGATTGCATCCGCATCTGCCTTCGGTGCTTCATCTGCCAGCGGGGA
AAAGAAAGAGATTTCCGCACCCCGCCTGCGCCAGCCTTCAAAAAGATGCATATAGGCAAAAGCAAAGGCA
TCGTCCCGCGCCACGGCAATGCGGTTTCCAAGCGGTTTCAGACGCGCGACATTGGCCATGGCGTCATAGC
GCTTCGGGCGTAGCCAGATGGTCTGAAGCGCATCCATGTCGATATGCGCTTCCATCACATCGGCCGCTTG
TTCGAGAAAGCTTTCAAGACCGGCATGTTCGTCCGCCTGCACCAGCCCCAGATGCCTTTCCGGCAAGGAA
AGTGCCGGATCTCGCGGCAGCGCGCCCAGAACCGGGACGCCAAGGGGTGCCAGAGCGCCCCGCAGCATGG
CTTCATGGCGGGGACTTCCGACACGATTGAGAATGACACCCTCAATCAGCACATCCTTGCGAAACTGGCT
GAAACCCCACACCAGCGCCGCGATGGAGTGGGATTGCCGGGCGCAATCCACCACCAGCACGACTGGCAAA
TCCAGAAGCCGCGCCAGATCGGCAGAAGAGCCCTTACCGTCAATCGCCCCGTCGAAAAGCCCCATCATGC
CTTCGGCGACAAGAACACGCGCGCCGCTTTCCGTCATGCGGGAGGAAAGCGCACTGATGAGTTCCGGTCG
CATGGCCCAGGGGTCGAGATTGAAACAATCCACGCCACTCGCCGCCCTGTGATAGGCGGGGTCGATATAA
TCCGGCCCGGCCTTCACCGGCGCAAGCACCTCGCCCCTGCGTTTCAGGGCGCGCAACAAACCCAAGGTCA
CGGTCGTCTTGCCCGACCCGGAGGCCGGTGCGGCAATCATGAATCCCTTCA

Protein Sequence : Show Sequence

>WP_002964414.1 MULTISPECIES: hydrogenobyrinate a,c-diamide synthase [Brucella]
MKGFMIAAPASGSGKTTVTLGLLRALKRRGEVLAPVKAGPDYIDPAYHRAASGVDCFNLDPWAMRPELIS
ALSSRMTESGARVLVAEGMMGLFDGAIDGKGSSADLARLLDLPVVLVVDCARQSHSIAALVWGFSQFRKD
VLIEGVILNRVGSPRHEAMLRGALAPLGVPVLGALPRDPALSLPERHLGLVQADEHAGLESFLEQAADVM
EAHIDMDALQTIWLRPKRYDAMANVARLKPLGNRIAVARDDAFAFAYMHLFEGWRRRGAEISFFSPLADE
APKADADAIYLPGGYPELHAQRLAGASRFRTAIGDAAARGVTVYGECGGYMVLGKTLEDAAGVHHPMLGL
LPLETSFARRKLHLGYRLLEPLGGLPWDMPLKAHEFHYASIVREEKADRLFRVRDASGENLGEAGLRVGS
VSGSFMHVIDFSGEAA

Molecule Role : Protective antigen
Molecule Role Annotation : Mice given BAB1_1316 (CobB) plus adjuvant could provide protection against virulent B. abortus infection, similarly with the known protective antigen Cu-Zn SOD and the license vaccine S19 (Fu et al., 2012).
Related Vaccine(s): Recombinant B. abortus CobB protein with CFA/IFA adjuvant

10. DnaK

Gene Name : DnaK
Sequence Strain (Species/Organism) : Brucella abortus bv. 1 str. 9-941
VO ID : VO_0010970
NCBI Gene ID : 3341115
NCBI Protein GI : 62290965
Locus Tag : BruAb1_2100
Genbank Accession : AE017223
Protein Accession : YP_222758
Taxonomy ID : 262698
Chromosome No : I
Gene Starting Position : 2066035
Gene Ending Position : 2067948
Gene Strand (Orientation) : +
Protein Name : molecular chaperone DnaK
Protein pI : 4.59
Protein Weight : 63035.04
Protein Length : 637
Protein Note : heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria

DNA Sequence : Show Sequence

>NC_006932.1:2066035-2067948 Brucella abortus biovar 1 str. 9-941 chromosome I, complete sequence
TATGGCTAAAGTTATTGGTATCGATCTGGGAACGACCAACTCCTGCGTTGCCGTCATGGACGGAAAGAAC
GCGAAGGTCATTGAAAATGCAGAAGGTGCACGCACCACCCCTTCGATCATCGCCTTCACCGATGGTGACG
AACGCCTCGCTGGTCAGCCTGCGAAGCGTCAGGCCGTCACCAACCCGGAAGGCACCCTTTTTGCCGTCAA
GCGCCTGATTGGCCGTCGCTATGACGATCCGATGGTCACCAAGGACAAGGATCTTGTTCCTTACAAGATC
GTCAAGGGCGACAATGGCGATGCCTGGGTCGAGGTGCACGGCAAGAAATATTCTCCGTCGCAGATCTCCG
CTATGATCCTTCAGAAGATGAAGGAAACGGCTGAATCCTACCTTGGCGAAACCGTTACGCAGGCCGTCAT
CACGGTTCCGGCCTACTTCAACGACGCCCAGCGTCAGGCCACCAAGGATGCCGGCAAGATCGCCGGTCTG
GAAGTTCTGCGCATCATCAACGAGCCGACCGCGGCTGCGCTTGCCTATGGTCTCGACAAGAGCGAAGGCA
AGACCATCGCTGTCTATGACCTTGGCGGCGGTACTTTCGACGTGTCGGTTCTGGAAATCGGCGACGGCGT
TTTTGAAGTGAAGTCCACCAATGGCGACACGTTCCTTGGCGGTGAAGACTTCGATATTCGTCTGGTCGAA
TATCTGGTTGCCGAGTTCAAGAAGGAAAGTGGCATCGACCTGAAGAACGACAAGCTTGCCCTGCAGCGCC
TCAAGGAAGCTGCCGAAAAGGCCAAGATCGAACTGTCGTCCTCGCAGCAGACCGAAATCAACCTGCCGTT
CATCACGGCTGACCAGACTGGCCCGAAGCATCTGGCGATCAAGCTGTCGCGCGCCAAGTTTGAAAGCCTG
GTCGATGATCTCGTGCAGCGCACGGTCGAGCCGTGCAAGGCGGCGCTCAAGGATGCCGGCCTCAAGGCTG
GCGAAATTGACGAAGTGGTTCTGGTCGGCGGCATGACCCGCATGCCCAAGATTCAGGAAGTCGTGAAGGC
CTTCTTCGGCAAGGAACCGCACAAGGGCGTGAACCCGGATGAAGTCGTGGCCATGGGCGCGGCGATCCAG
GGCGGCGTTTTGCAGGGCGACGTCAAGGACGTGCTGCTGCTCGACGTGACCCCGCTTTCGCTCGGCATTG
AAACGCTGGGCGGCGTGTTCACCCGCCTGATCGAACGCAACACCACTATCCCGACCAAGAAGTCGCAGAC
CTTCTCCACGGCTGAGGACAACCAGTCGGCCGTGACGATCCGCGTCTTCCAGGGCGAGCGTGAAATGGCA
GCCGATAACAAGCTGCTTGGACAGTTCGACCTCGTTGGCATTCCGCCCGCACCGCGCGGTGTGCCGCAGA
TCGAAGTGACCTTCGACATCGACGCCAACGGCATCGTCAACGTTTCGGCCAAGGATAAGGGTACCGGCAA
GGAGCACCAGATCCGCATTCAGGCTTCGGGCGGCCTGAGCGATGCCGACATCGAAAAGATGGTCAAGGAC
GCCGAAGCCAATGCCGAGGCCGACAAGAAGCGTCGTGAGAGCGTCGAAGCCAAGAATCAGGCCGAAAGCC
TGGTCCATTCGACCGAAAAGTCGCTGGCCGAATATGGCGACAAGGTTTCGGCCGATGACAAGAAGGCCAT
TGAGGATGCGATCGCAGCCCTCAAGACCTCGCTTGAAGGTGAAGACGCCGAGGATATCAAGGCGAAGACC
CAGGCCCTTGCCGAAGTTTCCATGAAGCTTGGTCAGGCCATGTACGAAGCAGCGCAGGCTGCCGAAGGTG
CGGGTGCCGAGGGCGGTGAACAGGCCTCTTCGTCCAAGGACGATGTGGTCGATGCCGACTACGAAGAAAT
CGACGACAACAAGAAGTCGTCGTA

Protein Sequence : Show Sequence

>YP_222758.1 molecular chaperone DnaK [Brucella abortus bv. 1 str. 9-941]
MAKVIGIDLGTTNSCVAVMDGKNAKVIENAEGARTTPSIIAFTDGDERLAGQPAKRQAVTNPEGTLFAVK
RLIGRRYDDPMVTKDKDLVPYKIVKGDNGDAWVEVHGKKYSPSQISAMILQKMKETAESYLGETVTQAVI
TVPAYFNDAQRQATKDAGKIAGLEVLRIINEPTAAALAYGLDKSEGKTIAVYDLGGGTFDVSVLEIGDGV
FEVKSTNGDTFLGGEDFDIRLVEYLVAEFKKESGIDLKNDKLALQRLKEAAEKAKIELSSSQQTEINLPF
ITADQTGPKHLAIKLSRAKFESLVDDLVQRTVEPCKAALKDAGLKAGEIDEVVLVGGMTRMPKIQEVVKA
FFGKEPHKGVNPDEVVAMGAAIQGGVLQGDVKDVLLLDVTPLSLGIETLGGVFTRLIERNTTIPTKKSQT
FSTAEDNQSAVTIRVFQGEREMAADNKLLGQFDLVGIPPAPRGVPQIEVTFDIDANGIVNVSAKDKGTGK
EHQIRIQASGGLSDADIEKMVKDAEANAEADKKRRESVEAKNQAESLVHSTEKSLAEYGDKVSADDKKAI
EDAIAALKTSLEGEDAEDIKAKTQALAEVSMKLGQAMYEAAQAAEGAGAEGGEQASSSKDDVVDADYEEI
DDNKKSS

Molecule Role : Protective antigen
Molecule Role Annotation : Brucella DnaK is a protective antigen (Delpino et al., 2007b).
COG: COG0443O, under O: Posttranslational modification, protein turnover, chaperones

11. Gap

Gene Name : Gap
Sequence Strain (Species/Organism) : Brucella abortus bv. 1 str. 9-941
VO ID : VO_0012375
NCBI Gene ID : 3340207
NCBI Protein GI : 62290600
Locus Tag : BruAb1_1713
Genbank Accession : AE017223
Protein Accession : YP_222393
Taxonomy ID : 262698
Chromosome No : I
Gene Starting Position : 1684720
Gene Ending Position : 1685727
Gene Strand (Orientation) : +
Protein Name : glyceraldehyde-3-phosphate dehydrogenase
Protein pI : 6.44
Protein Weight : 32765.28
Protein Length : 335
Protein Note : catalyzes the formation of 3-phospho-D-glyceroyl phosphate from D-glyceraldehyde 3-phosphate

DNA Sequence : Show Sequence

>NC_006932.1:1684720-1685727 Brucella abortus biovar 1 str. 9-941 chromosome I, complete sequence
AATGGCAGTTCGCGTCGCAATCAACGGTTTTGGCCGCATTGGCCGTAACATCCTTCGCGCCATCGTGGAA
TCGGGGCGCACCGACATTCAGGTCGTCGCCATCAACGATCTCGGCCCGGTCGAAACCAACGCACATCTTC
TGCGTTATGACAGCGTTCATGGCCGTTTCCCCAAGGAAGTGGAGGTTGCAGGCGATACGATCGATGTTGG
CTACGGCCCGATCAAGGTTCATGCCGTCCGCAACCCGGCCGAACTGCCGTGGAAGGAAGAAAACGTCGAT
ATCGCCCTTGAATGCACCGGCATTTTCACCTCGCGCGACAAGGCAGCACTTCATCTTGAAGCTGGCGCCA
AGCGCGTCATCGTCTCCGCTCCCGCAGACGGTGCCGATCTCACCGTCGTCTATGGTGTCAACAACGACAA
GCTGACGAAGGACCATCTGGTCATCTCCAACGCTTCGTGTACCACCAACTGCCTTGCGCCGGTGGCTCAG
GTTCTCAACGATACTATCGGTATCGAAAAGGGCTTCATGACCACGATCCACTCCTATACGGGCGACCAGC
CGACGCTGGACACCATGCACAAGGATCTCTACCGCGCCCGCGCCGCTGCCCTTTCCATGATCCCGACCTC
GACGGGTGCGGCCAAGGCCGTCGGTCTCGTTCTGCCGGAACTGAAAGGCAAGCTCGACGGCGTTGCCATT
CGCGTCCCGACCCCAAATGTCTCGGTCGTTGATCTCACCTTCATCGCCAAGCGTGAAACCACCGTTGAAG
AAGTCAACAATGCGATCCGCGAAGCCGCCAATGGCCGCCTCAAGGGCATTCTCGGCTATACCGATGAGAA
GCTCGTCTCGCACGACTTCAACCACGATTCCCATTCCTCGGTCTTCCACACCGACCAGACCAAGGTTATG
GACGGCACCATGGTGCGTATCCTGTCGTGGTACGACAATGAATGGGGCTTCTCCAGCCGCATGAGCGACA
CCGCCGTCGCTTTGGGCAAGCTGATCTG

Protein Sequence : Show Sequence

>YP_222393.1 glyceraldehyde-3-phosphate dehydrogenase [Brucella abortus bv. 1 str. 9-941]
MAVRVAINGFGRIGRNILRAIVESGRTDIQVVAINDLGPVETNAHLLRYDSVHGRFPKEVEVAGDTIDVG
YGPIKVHAVRNPAELPWKEENVDIALECTGIFTSRDKAALHLEAGAKRVIVSAPADGADLTVVYGVNNDK
LTKDHLVISNASCTTNCLAPVAQVLNDTIGIEKGFMTTIHSYTGDQPTLDTMHKDLYRARAAALSMIPTS
TGAAKAVGLVLPELKGKLDGVAIRVPTPNVSVVDLTFIAKRETTVEEVNNAIREAANGRLKGILGYTDEK
LVSHDFNHDSHSSVFHTDQTKVMDGTMVRILSWYDNEWGFSSRMSDTAVALGKLI

Molecule Role : Protective antigen
Molecule Role Annotation : GAPDH (Gap) associated with murine IL-12 gene in a DNA vaccine formulation partially protected mice against experimental infection (Rosinha et al., 2002b).

12. IalB

Gene Name : IalB
Sequence Strain (Species/Organism) : Brucella melitensis bv. 1 str. 16M
VO ID : VO_0010967
NCBI Gene ID : 1197295
NCBI Protein GI : 17987867
Locus Tag : BMEI1584
Genbank Accession : AE008917
Protein Accession : NP_540501
Taxonomy ID : 224914
Chromosome No : I
Gene Starting Position : 1632893
Gene Ending Position : 1633414
Gene Strand (Orientation) : +
Protein Name : invasion protein B
Protein pI : 8.98
Protein Weight : 17016.04
Protein Length : 173

DNA Sequence : Show Sequence

>NC_003317.1:1632893-1633414 Brucella melitensis bv. 1 str. 16M chromosome I, complete sequence
TATGAAAAATTATCGTGCAATCGGTCTTGCATTCACGTTCACTGCCCTTTCCAGTCTTTCGGCCTTTGCA
GCCTCCCTGCCCGGCGGAGCAAGCACCTTGCAGGAAACCTATCAGGACTGGACCGTGTCTTGCCAGTCGC
AGAAGGATACAACAGCCTGCGTGATGCGTCAGGAGCAAAGCAGCGCCCAGGCCGGCCAGCGCGTTCTGAC
TGCCGAGCTGCGCAACGTCGCCGGCGGCAAAGTTGACGGTGTGTTGCTGATGCCGTTCGGTCTTGATCTT
GCCAAGGGCGCCTCGCTCAAGATTGATGACACCGCTGGGCCAAACCTCACCTTCTCCACCTGCCTGCCGC
AGGGCTGCCTCGCGCCAGTGAGCTTCGATGCCAAGCAGGTTGCTGCGCTGAAATCCGGCACCAACATCAA
TGTCACCACGACGGCGCTCAGCCCGAGCCAGCCGGTTGCCTTCAAGATTTCCCTGAAAGGCTTCGGTGCC
GCGCTCGACCGCATTCAGGCATTGACCAAGTA

Protein Sequence : Show Sequence

>NP_540501.1 invasion protein B [Brucella melitensis bv. 1 str. 16M]
MKNYRAIGLAFTFTALSSLSAFAASLPGGASTLQETYQDWTVSCQSQKDTTACVMRQEQSSAQAGQRVLT
AELRNVAGGKVDGVLLMPFGLDLAKGASLKIDDTAGPNLTFSTCLPQGCLAPVSFDAKQVAALKSGTNIN
VTTTALSPSQPVAFKISLKGFGAALDRIQALTK

Molecule Role : Protective antigen
Molecule Role Annotation : Brucella IalB is a protective antigen (Commander et al., 2007).
COG: COG5342R, under R: General function prediction only
Related Vaccine(s): B. melitensis DNA vaccine p-ialB

13. L7/L12

Gene Name : L7/L12
Sequence Strain (Species/Organism) : Brucella abortus RB51-AHVLA
NCBI Protein GI : 479804332
Other Database IDs : CDD:100102
Taxonomy ID : 1198700
Gene Strand (Orientation) : ?
Protein Name : 50S ribosomal protein L7/L12
Protein pI : 4.62
Protein Weight : 12562.32
Protein Length : 198
Protein Note : vaccine strain

Protein Sequence : Show Sequence

>ENT73302.1 50S ribosomal protein L7/L12 [Brucella abortus RB51-AHVLA]
MADLAKIVEDLSALTVLEAAELSKLLEEKWGVSAAAPVAVAAAGGAAPAAAAEEKTEFDVVLADGGANKI
NVIKEVRALTGLGLKEAKDLVEGAPKAVKEGASKDEAEKIKAQLEAAGAKVELK

Molecule Role : Protective antigen
Related Vaccine(s): Divalent DNA B. abortus Vaccine pcDNA3.1-L7/L12-Omp16

14. Omp16

Gene Name : Omp16
Sequence Strain (Species/Organism) : Brucella abortus 2308
VO ID : VO_0010973
NCBI Gene ID : 3788229
NCBI Protein GI : 489056820
Locus Tag : BAB_RS24085
Genbank Accession : AM040264
Protein Accession : WP_002966947
Taxonomy ID : 359391
Chromosome No : I
Gene Starting Position : 1654432
Gene Ending Position : 1654938
Gene Strand (Orientation) : -
Protein Name : hypothetical protein
Protein pI : 10.24
Protein Weight : 17438.15
Protein Length : 168
Protein Annotation : Synonyms: Omp16, MotY

DNA Sequence : Show Sequence

>NC_007618.1:1654432-1654938 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence, strain 2308
TTTACCGTCCGGCCCCGTTGAGAACGGTGACGGCGCGACGGTTCTGCGACCAGCATGTGTCGGCATCGCA
GACGGCAACCGGGCGCTCATTACCGTAGGAAATGGTGCGCATGCGGTTGGTCGGCACACCGCGCGAAGCG
AGGAAGTCGCGGGTGGCGGCAGCACGGCGCTGGCCAAGGGCGAGGTTGTACTCACGCGTGCCGCGCTCGT
CGGCATGGCCTTCGATCGTGATCGAATACTGCGGATAACGCTGCAACCACTGGGCCTGCTTGGAAAGCGT
CTGCTGCGCATCGGCGCGGATCAGCGACGAATCGAGATCGAAGAAGATGCGGTCGCCGACATTAACGGTG
AAGTCCTGCGAGGAGCCCGGCGTTGCAGCGCCTGCACCGAGACCCAGATCACCGGCATTATTCGGAAGGT
TCTTCTTTGACGCACAGCCGGCAACGGCGAGCGACATGAAAAGCGCAATAGCGATCGGGCTACGTGCAAT
CGACTGGATACGGCGCA

Protein Sequence : Show Sequence

>WP_002966947.1 MULTISPECIES: hypothetical protein [Brucella]
MRRIQSIARSPIAIALFMSLAVAGCASKKNLPNNAGDLGLGAGAATPGSSQDFTVNVGDRIFFDLDSSLI
RADAQQTLSKQAQWLQRYPQYSITIEGHADERGTREYNLALGQRRAAATRDFLASRGVPTNRMRTISYGN
ERPVAVCDADTCWSQNRRAVTVLNGAGR

Molecule Role : Protective antigen
Molecule Role Annotation : Brucella Omp16 is a protective antigen (Pasquevich et al., 2009).
COG: COG2885M, under M: Cell wall/membrane/envelope biogenesis
Related Vaccine(s): B. abortus DNA vaccine encoding RplL and Omp16 , B. abortus Omp16+IFA vaccines

15. omp16

Gene Name : omp16
Sequence Strain (Species/Organism) : Brucella abortus RB51-AHVLA
NCBI Protein GI : 479804733
Other Database IDs : CDD:225439
CDD:143586
Taxonomy ID : 1198700
Gene Strand (Orientation) : ?
Protein Name : outer membrane lipoprotein omp16
Protein pI : 10.24
Protein Weight : 18331.05
Protein Length : 247
Protein Note : vaccine strain

Protein Sequence : Show Sequence

>ENT73703.1 outer membrane lipoprotein omp16 [Brucella abortus RB51-AHVLA]
MRRIQSIARSPIAIALFMSLAVAGCASKKNLPNNAGDLGLGAGAATPGSSQDFTVNVGDRIFFDLDSSLI
RADAQQTLSKQAQWLQRYPQYSITIEGHADERGTREYNLALGQRRAAATRDFLASRGVPTNRMRTISYGN
ERPVAVCDADTCWSQNRRAVTVLNGAGR

Molecule Role : Protective antigen
Related Vaccine(s): Divalent DNA B. abortus Vaccine pcDNA3.1-L7/L12-Omp16

16. Omp19

Gene Name : Omp19
Sequence Strain (Species/Organism) : Brucella abortus 2308
VO ID : VO_0010972
NCBI Gene ID : 3788831
NCBI Protein GI : 489054833
Locus Tag : BAB_RS25135
Genbank Accession : AM040264
Protein Accession : WP_002964998
Taxonomy ID : 359391
Chromosome No : I
Gene Starting Position : 1877306
Gene Ending Position : 1877839
Gene Strand (Orientation) : -
Protein Name : hypothetical protein
Protein pI : 8.86
Protein Weight : 16412.87
Protein Length : 177

DNA Sequence : Show Sequence

>NC_007618.1:1877306-1877839 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence, strain 2308
ATCAGCGCGACAGCGTCACGGCCTGCCCGCCGGTGGTCTGGCCATCGAAGCGGCCCTGTCCTGAAGAATA
GAGCGAGGCAACCGTACCGCCGTTCGCATCGTAAAGGACGAGTTGCTTGCCATTGACGGCCCAGGAGGCA
AGATTAGCCAGTTCACCGGGGCAGCGCAGCGGGCCTGCGCGATAGCCCTGGCCATATTTGGTCTGCGGCG
TCGCGATCTTGCAGCTCTGACCACCAAGCGAGGCGTTCCAGACGCCAGCCACGGCGCCGGGCGTCAGGTC
CGGTGCGGATGCAGGCGGCAGGCTTGCGACCTGTGTGCCGGATTGCGCGCTCATATCCGTGGAGGGCGCA
TTGGGGAATTGTGTGGGAGAATCAAGATTGCCTTTCTGCACCGTGCCTGCCGGAACAGCATTGACCGGTG
CAGGCGGCGGCGGAGGCGAAACATTATCGAGATTACCAAGCCGGGAGCTCTGGCACCCGGCCAGGACAAT
GCCAGCCGCCGCGAGGCTGAGCAGACTTGCTTTTGAAATTCCCA

Protein Sequence : Show Sequence

>WP_002964998.1 MULTISPECIES: hypothetical protein [Brucella]
MGISKASLLSLAAAGIVLAGCQSSRLGNLDNVSPPPPPAPVNAVPAGTVQKGNLDSPTQFPNAPSTDMSA
QSGTQVASLPPASAPDLTPGAVAGVWNASLGGQSCKIATPQTKYGQGYRAGPLRCPGELANLASWAVNGK
QLVLYDANGGTVASLYSSGQGRFDGQTTGGQAVTLSR

Molecule Role : Protective antigen
Molecule Role Annotation : Brucella Omp19 is a protective antigen (Pasquevich et al., 2009).
Related Vaccine(s): B. abortus Omp16+IFA vaccines , B. abortus Omp19 + IFA vaccine

17. Omp25

Gene Name : Omp25
Sequence Strain (Species/Organism) : Brucella melitensis bv. 1 str. 16M
VO ID : VO_0010966
NCBI Gene ID : 1196960
NCBI Protein GI : 17987532
Locus Tag : BMEI1249
Genbank Accession : AE008917
Protein Accession : NP_540166
Taxonomy ID : 224914
Chromosome No : I
Gene Starting Position : 1297524
Gene Ending Position : 1298165
Gene Strand (Orientation) : -
Protein Name : hypothetical protein
Protein pI : 9.03
Protein Weight : 21696.72
Protein Length : 213
Protein Annotation : Reference: http://www.ncbi.nlm.nih.gov/pubmed/17049676

DNA Sequence : Show Sequence

>NC_003317.1:1297524-1298165 Brucella melitensis bv. 1 str. 16M chromosome I, complete sequence
ATTAGAACTTGTAGCCGATGCCGACGCGGAAATCCTGCGTGTCCAGCTTGTTGCGAACAGTCGTACCGGC
CAGATCATAGTTCTTGTTGCCGTACTGGGTGTAACGGTACTCAACGCGGCCGAGGATGTTGTCCGTCAGC
TTGGCTTCGAGACCGGCACCAGCCGTCCAACCCACGCGGAACTTGCTTTCGTCGTCCAAGCCGTTGTTAA
GCTTGATCTGCGAACCGGCAATACCAGCCGTGAGGTACGGCATAACCGGGTTCAGGTCGTAGCCAACGCG
GGCACGCAGCGAGCCTTCAAAGCCCTGCTTGACTTCCAGGCCGTCCTTGGACTTCTTGGCCCAGGAATAA
CCTGCATCACCTTCAACACCGTATACGATCTGGTCCTGCTGGAAGTTCCAGCCAGCAAAGGCGCCAGCCT
TCCAATCGTCAGGCTTGATGCTGCCAACGGTGCTGGTCTTGGCCTTGTTCCAGCCGTAGCCAAGGTAAAG
ACCGGTATAGCCACCAGCCCAGCTATACTGGGGAGCTACTTCAACCGGAGCCGGAACCGGAGGCTGTTCC
TGGATGGCGTCGGCAGCAAAAGCGGTCGCAGAGAACGGCAGCAACGCAGCCGAGACGATTACGAGAGACT
TAAGAGTGCGCA

Protein Sequence : Show Sequence

>NP_540166.1 hypothetical protein BMEI1249 [Brucella melitensis bv. 1 str. 16M]
MRTLKSLVIVSAALLPFSATAFAADAIQEQPPVPAPVEVAPQYSWAGGYTGLYLGYGWNKAKTSTVGSIK
PDDWKAGAFAGWNFQQDQIVYGVEGDAGYSWAKKSKDGLEVKQGFEGSLRARVGYDLNPVMPYLTAGIAG
SQIKLNNGLDDESKFRVGWTAGAGLEAKLTDNILGRVEYRYTQYGNKNYDLAGTTVRNKLDTQDFRVGIG
YKF

Molecule Role : Protective antigen
Molecule Role Annotation : Brucella Omp25 is a protective antigen (Commander et al., 2007).
Additional Molecule Role : Virmugen
Additional Molecule Role Annotation : A omp25 mutant is attenuated and provides protection against challenge in mice (Edmonds et al., 2002).
COG: COG3637M, under M: Cell wall/membrane/envelope biogenesis
Related Vaccine(s): B. melitensis DNA vaccine p-Omp25 , Brucella melitensis omp25 mutant vaccine

18. Omp31

Gene Name : Omp31
Sequence Strain (Species/Organism) : Brucella abortus 2308
VO ID : VO_0010939
NCBI Gene ID : 3788834
NCBI Protein GI : 489054549
Locus Tag : BAB_RS23735
Genbank Accession : AM040264
Protein Accession : WP_002964719
Taxonomy ID : 359391
Chromosome No : I
Gene Starting Position : 1583642
Gene Ending Position : 1584427
Gene Strand (Orientation) : +
Protein Name : membrane protein
Protein pI : 4.74
Protein Weight : 25591.47
Protein Length : 261

DNA Sequence : Show Sequence

>NC_007618.1:1583642-1584427 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence, strain 2308
CATGTTTAGCTTAAAAGGGACTGTTATGAAAACCGCACTTCTTGCATCCGTCGCAATGTTGTTCACAAGC
TCGGCTATGGCTGCCGACATCATCGTTGCTGAACCGGCACCCGTTGCAGTCGACACGTTCTCTTGGACTG
GCGGCTATATTGGTATCAATGCTGGTTACGCTGGCGGCAAGTTCAAGCATCCGTTCTCAGGCATCGAGCA
GGATGGGGCCCAAGATTTTTCAGGTTCGCTCGACGTCACGGCCAGCGGCTTTGTTGGCGGCGTTCAGGCC
GGTTATAACTGGCAGCTTGCCAACGGCCTCGTGCTTGGTGGCGAAGCTGACTTCCAGGGCTCGACGGTTA
AGAGCAAGCTTGTTGACAACGGTGACCTCTCCGATATCGGCGTTGCAGGCAACCTCAGCGGCGACGAAAG
CTTCGTCCTCGAGACCAAGGTTCAGTGGTTTGGAACGGTGCGTGCGCGCCTCGGCTTCACCCCGACTGAA
CGCCTGATGGTCTATGGTACCGGTGGTTTGGCCTATGGTAAGGTCAAGACGTCGCTTAGCGCCTATGACG
ATGGTGAATCGTTCAGCGCCGGAAACTCTAAGACCAAGGCTGGCTGGACGCTTGGTGCAGGTGTAGAATA
CGCCGTCACCAACAATTGGACCCTGAAGTCGGAATACCTCTACACCGACCTCGGCAAGCGTTCCTTCAAT
TACATTGATGAAGAAAACGTCAATATTAACATGGAAAACAAGGTGAACTTCCACACCGTCCGCCTCGGTC
TGAACTACAAGTTCTA

Protein Sequence : Show Sequence

>WP_002964719.1 MULTISPECIES: porin family protein [Brucella]
MFSLKGTVMKTALLASVAMLFTSSAMAADIIVAEPAPVAVDTFSWTGGYIGINAGYAGGKFKHPFSGIEQ
DGAQDFSGSLDVTASGFVGGVQAGYNWQLANGLVLGGEADFQGSTVKSKLVDNGDLSDIGVAGNLSGDES
FVLETKVQWFGTVRARLGFTPTERLMVYGTGGLAYGKVKTSLSAYDDGESFSAGNSKTKAGWTLGAGVEY
AVTNNWTLKSEYLYTDLGKRSFNYIDEENVNINMENKVNFHTVRLGLNYKF

Molecule Role : Protective antigen
COG: COG3637M, under M: Cell wall/membrane/envelope biogenesis
Related Vaccine(s): B. melitensis 16M DNA vaccine expressing Omp31

19. omp31

Gene Name : omp31
Sequence Strain (Species/Organism) : Brucella melitensis bv. 1 str. 16M
NCBI Protein GI : 61229373
Other Database IDs : CDD:226163
Taxonomy ID : 224914
Gene Strand (Orientation) : ?
Protein Name : 31 kDa outer-membrane immunogenic protein
Protein pI : 5.06
Protein Weight : 24732.46
Protein Length : 343
Protein Note : biovar: 1 str. 16M

Protein Sequence : Show Sequence

>sp|P0A3U4.1|OM31_BRUME RecName: Full=31 kDa outer-membrane immunogenic protein; Flags: Precursor
MKSVILASIAAMFATSAMAADVVVSEPSAPTAAPVDTFSWTGGYIGINAGYAGGKFKHPFSSFDKEDNEQ
VSGSLDVTAGGFVGGVQAGYNWQLDNGVVLGAETDFQGSSVTGSISAGASGLEGKAETKVEWFGTVRARL
GYTATERLMVYGTGGLAYGKVKSAFNLGDDASALHTWSDKTKAGWTLGAGAEYAINNNWTLKSEYLYTDL
GKRNLVDVDNSFLESKVNFHTVRVGLNYKF

Molecule Role : Protective antigen
Related Vaccine(s): B. melitensis DNA vaccine pCI-Omp31 , B. melitensis DNA vaccine pCIBLSOmp31 encoding BLSOmp31 (fusion protein)

20. ORF

Gene Name : ORF
VO ID : VO_0010927
NCBI Nucleotide GI : 599653
NCBI Protein GI : 599654
Protein Accession : CAA86936.1
3D structure: PDB ID : 1DI0
Other Database IDs : CDD:237096
CDD:187741
GOA:P61711
InterPro: IPR002180
PDB: 1DI0
PDB: 1T13
PDB: 1XN1
UniProtKB/UniProt: P61711
Taxonomy ID : 235
Gene Strand (Orientation) : ?
Protein pI : 7.41
Protein Weight : 16119.15
Protein Length : 197
Protein Note : riboflavin synthase subunit beta; Provisional

DNA Sequence : Show Sequence

>gi|599653|emb|Z46864.1| B.abortus gene for open reading frame
GAATTCCGATCAGTGCATAGTTTCCGCGTGCTCGCGCAATGGTGCGCGGGCTTGTTCTCGGGGCGGGGTG
AAACTCCCCACCGGCGGTATGAAAAGCAATTTTCAAGCCCGCGAGCGCCTGAAATGGAAGCCGATTCGCA
TGCCATTTCAGGGTCAGCAGATCCGGTGAGATGCCGGAGCCGACGGTTAAAGTCCGGATGGAAGAGAGCG
AATGAGCGTCACGATTGCGCCTTCCGGCGTCGTTCTTGCGTTCTTTTGTGCGCCCTGATTCTAGTTTCGT
GAGGAACCTATGAACCAAAGCTGTCCGAACAAGACATCCTTTAAAATCGCATTCATTCAGGCCCGCTGGC
ACGCCGACATCGTTGACGAAGCGCGCAAAAGCTTTGTCGCCGAACTGGCCGCAAAGACGGGTGGCAGCGT
CGAGGTAGAGATATTCGACGTGCCGGGTGCATATGAAATTCCCCTTCACGCCAAGACATTGGCCAGAACC
GGGCGCTATGCAGCCATCGTCGGTGCGGCCTTCGTGATCGACGGCGGCATCTATCGTCATGATTTCGTGG
CGACGGCCGTTATCAACGGCATGATGCAGGTGCAGCTTGAAACGGAAGTGCCGGTGCTGAGCGTCGTGCT
GACGCCGCACCATTTCCATGAAAGCAAGGAGCATCACGACTTCTTCCATGCTCATTTCAAGGTGAAGGGC
GTGGAAGCGGCCCATGCCGCCTTGCAGATCGTGAGCGAGCGCAGCCGCATCGCCGCGCTTGTCTGACTAA
CCCTCTATAATACGCCCGCAATGGGTATAAATGTCGAATTC

Protein Sequence : Show Sequence

>CAA86936.1 ORF [Brucella abortus]
MNQSCPNKTSFKIAFIQARWHADIVDEARKSFVAELAAKTGGSVEVEIFDVPGAYEIPLHAKTLARTGRY
AAIVGAAFVIDGGIYRHDFVATAVINGMMQVQLETEVPVLSVVLTPHHFHESKEHHDFFHAHFKVKGVEA
AHAALQIVSERSRIAALV

Molecule Role : Protective antigen
Molecule Role Annotation : This gene was originally isolated from a lambda gt11 expression library probed with sera from Brucella-infected sheep. Sequence analysis of the cloned gene revealed the presence of an open reading frame of 158 amino acids encoding a protein of 17.3 kDa (Hemmen et al., 1995). It was later identified as a cytoplasmic protein lumazine synthase (Goldbaum et al., 1999). This gene can be used for diagnosis (Hemmen et al., 1995; Goldbaum et al., 1999) and vaccine development (Velikovsky et al., 2002).
Related Vaccine(s): B. abortus pcDNA-BLS , B. melitensis DNA vaccine pCIBLSOmp31 encoding BLSOmp31 (fusion protein)

21. P39 from Brucella abortus

Gene Name : P39 from Brucella abortus
Sequence Strain (Species/Organism) : Brucella abortus bv. 1 str. 9-941
NCBI Protein GI : 68067499
Other Database IDs : CDD:224567
Taxonomy ID : 262698
Gene Strand (Orientation) : ?
Protein Name : Probable sugar-binding periplasmic protein
Protein pI : 5.06
Protein Weight : 44330.68
Protein Length : 589
Protein Note : biovar: 1 str. 9-941

Protein Sequence : Show Sequence

>sp|O06875.3|SP39_BRUAB RecName: Full=Probable sugar-binding periplasmic protein; AltName: Full=Immunogenic 39 kDa protein; AltName: Full=P39; Flags: Precursor
MHKLLKLAAMGTAACALLAGMAPVANAQEKQNVEVLHWWTSGGEASALEVLKKDLESKGISWTDMPVAGG
GGTEAMTVLRARVTAGNAPTAVQMLGFDIRDWAEQGALGNLDTVASKEGWEKVIPAPLQEFAKYDGHWIA
APVNIHSTNWMWINKAALDKAGGKEPTNWDELIALLDNFKAQGITPIAHGGQPWQDATIFDAVVLSFGPD
FYKKAFIDLDPEALGSDTMKQAFDRMSKLRTYVDDNFSGRDWNLASAMVIEGKAGVQFMGDWAKGEFLKA
GKKPGEDFVCMRYPGTQGAVTFNSDMFAMFKVSEDKVPAQLEMASAIESPAFQSAFNVVKGSAPARTDVP
DTAFDACGKKAIADVKEANSKGTLLGSMAHGYANPAAVKNAIYDVVTRQFNGQLSSEDAVKELVAAVEAA
K

Molecule Role : Protective antigen
Related Vaccine(s): B. abortus DNA vaccine pcDNA3.1-p39-L7/L12 encoding p39-L7/L12 fusion protein

22. PALFn

Gene Name : PALFn
Sequence Strain (Species/Organism) : Bacillus anthracis
NCBI Protein GI : AKB95640
Other Database IDs : CDD:284994
CDD:281492
CDD:285037
CDD:286266
CDD:238144
Taxonomy ID : 32630
Protein Name : PALFn
Protein pI : 5.69
Protein Weight : 169963.43
Protein Length : 1613
Protein Note : derived from Bacillus anthracis

Protein Sequence : Show Sequence

>AKB95640.1 PALFn [synthetic construct]
MSYYHHHHHHDYDIPTTENLYFQGAMDPEVKQENRLLNESESSSQGLLGYYFSDLNFQAPMVVTSSTTGD
LSIPSSELENIPSENQYFQSAIWSGFIKVKKSDEYTFATSADNHVTMWVDDQEVINKASNSNKIRLEKGR
LYQIKIQYQRENPTEKGLDFKLYWTDSQNKKEVISSDNLQLPELKQKSSNSRKKRSTSAGPTVPDRDNDG
IPDSLEVEGYTVDVKNKRTFLSPWISNIHEKKGLTKYKSSPEKWSTASDPYSDFEKVTGRIDKNVSPEAR
HPLVAAYPIVHVDMENIILSKNEDQSTQNTDSQTRTISKNTSTSRTHTSEVHGNAEVHASFFDIGGSVSA
GFSNSNSSTVAIDHSLSLAGERTWAETMGLNTADTARLNANIRYVNTGTAPIYNVLPTTSLVLGKNQTLA
TIKAKENQLSQILAPNNYYPSKNLAPIALNAQDDFSSTPITMNYNQFLELEKTKQLRLDTDQVYGNIATY
NFENGRVRVDTGSNWSEVLPQIQETTARIIFNGKDLNLVERRIAAVNPSDPLETTKPDMTLKEALKIAFG
FNEPNGNLQYQGKDITEFDFNFDQQTSQNIKNQLAELNVTNIYTVLDKIKLNAKMNILIRDKRFHYDRNN
IAVGADESVVKEAHREVINSSTEGLLLNIDKDIRKILSGYIVEIEDTEGLKEVINDRYDMLNISSLRQDG
KTFIDFKKYNDKLPLYISNPNYKVNVYAVTKENTIINPSENGDTSTNGIKKILIFSKKGYEIGGGGGSGG
GGSAGGHGDVGMHVKEKEKNKDENKRKDEERNKTQEEHLKEIMKHIVKIEVKGEEAVKKEAAEKLLEKVP
SDVLEMYKAIGGKIYIVDGDITKHISLEALSEDKKKIKDIYGKDALLHEHYVYAKEGYEPVLVIQSSEDY
VENTEKALNVYYEIGKILSRDILSKINQPYQKFLDVLNTIKNASDSDGQDLLFTNQLKEHPTDFSVEFLE
QNSNEVQEVFAKAFAYYIEPQHRDVLQLYAPEAFNYMDKFNEQEINLSLEELKDQRMLSRYEKWEKIKQH
YQHWSDSLSEEGRGLLKKLQIPIEPKKDDIIHSLSQEEKELLKRIQIDSSDFLSTEEKEFLKKLQIDIRD
SLSEEEKELLNRIQVDSSNPLSEKEKEFLKKLKLDIQPYDINQRLQDTGGLIDSPSINLDVRKQYKRDIQ
NIDALLHQSIGSTLYNKIYLYENMNINNLTATLGADLVDSTDNTKINRGIFNEFKKNFKYSISSNYMIVD
INERPALDNERLKWRIQLSPDTRAGYLENGKLILQRNIGLEIKDVQIIKQSEKEYIRIDAKVVPKSKIDT
KIQEAQLNINQEWNKALGLPKYTKLITFNVHNRYASNIVESAYLILNEWKNNIQSDLIKKVTNYLVDGNG
RFVFTDITLPNIAEQYTHQDEIYEQVHSKGLYVPESRSILLHGPSKGVELRNDSEGFIHEFGHAVDDYAG
YLLDKNQSDLVTNSKKFIDIFKEEGSNLTSYGRTNEAEFFAEAFRLMHSTDHAERLKVQKNAPKTFQFIN
DQIKFIINS

Molecule Role : Protective antigen
Molecule Role Annotation : (Suryanarayana et al., 2016)

23. RplL

Gene Name : RplL
Sequence Strain (Species/Organism) : Brucella abortus 2308
VO ID : VO_0010908
NCBI Gene ID : 3788918
NCBI Protein GI : 489054195
Locus Tag : BAB_RS21965
Genbank Accession : AM040264
Protein Accession : WP_002964371
Taxonomy ID : 359391
Chromosome No : I
Gene Starting Position : 1229918
Gene Ending