|
|
Listeria monocytogenes |
| Table of Contents |
- Protective Antigens
- Hly
- iap
|
| I. Vaccine Related Protective Antigens |
|
1. Hly
|
-
Gene Name :
Hly
-
Sequence Strain (Species/Organism) :
Listeria monocytogenes EGD-e
-
VO ID :
VO_0012388
-
NCBI Gene ID :
987033
-
NCBI Protein GI :
16802248
-
Locus Tag :
lmo0202
-
Genbank Accession :
AL591824
-
Protein Accession :
NP_463733
-
Taxonomy ID :
169963
-
Gene Starting Position :
205818
-
Gene Ending Position :
207407
-
Gene Strand (Orientation) :
+
-
Protein Name :
listeriolysin O precursor
-
Protein pI :
8.23
-
Protein Weight :
54322.11
-
Protein Length :
529
-
DNA Sequence : Show Sequence
>NC_003210.1:205818-207407 Listeria monocytogenes EGD-e chromosome, complete genome
CATGAAAAAAATAATGCTAGTTTTTATTACACTTATATTAGTTAGTCTACCAATTGCGCAACAAACTGAA
GCAAAGGATGCATCTGCATTCAATAAAGAAAATTCAATTTCATCCATGGCACCACCAGCATCTCCGCCTG
CAAGTCCTAAGACGCCAATCGAAAAGAAACACGCGGATGAAATCGATAAGTATATACAAGGATTGGATTA
CAATAAAAACAATGTATTAGTATACCACGGAGATGCAGTGACAAATGTGCCGCCAAGAAAAGGTTACAAA
GATGGAAATGAATATATTGTTGTGGAGAAAAAGAAGAAATCCATCAATCAAAATAATGCAGACATTCAAG
TTGTGAATGCAATTTCGAGCCTAACCTATCCAGGTGCTCTCGTAAAAGCGAATTCGGAATTAGTAGAAAA
TCAACCAGATGTTCTCCCTGTAAAACGTGATTCATTAACACTCAGCATTGATTTGCCAGGTATGACTAAT
CAAGACAATAAAATCGTTGTAAAAAATGCCACTAAATCAAACGTTAACAACGCAGTAAATACATTAGTGG
AAAGATGGAATGAAAAATATGCTCAAGCTTATCCAAATGTAAGTGCAAAAATTGATTATGATGACGAAAT
GGCTTACAGTGAATCACAATTAATTGCGAAATTTGGTACAGCATTTAAAGCTGTAAATAATAGCTTGAAT
GTAAACTTCGGCGCAATCAGTGAAGGGAAAATGCAAGAAGAAGTCATTAGTTTTAAACAAATTTACTATA
ACGTGAATGTTAATGAACCTACAAGACCTTCCAGATTTTTCGGCAAAGCTGTTACTAAAGAGCAGTTGCA
AGCGCTTGGAGTGAATGCAGAAAATCCTCCTGCATATATCTCAAGTGTGGCGTATGGCCGTCAAGTTTAT
TTGAAATTATCAACTAATTCCCATAGTACTAAAGTAAAAGCTGCTTTTGATGCTGCCGTAAGCGGAAAAT
CTGTCTCAGGTGATGTAGAACTAACAAATATCATCAAAAATTCTTCCTTCAAAGCCGTAATTTACGGAGG
TTCCGCAAAAGATGAAGTTCAAATCATCGACGGCAACCTCGGAGACTTACGCGATATTTTGAAAAAAGGC
GCTACTTTTAATCGAGAAACACCAGGAGTTCCCATTGCTTATACAACAAACTTCCTAAAAGACAATGAAT
TAGCTGTTATTAAAAACAACTCAGAATATATTGAAACAACTTCAAAAGCTTATACAGATGGAAAAATTAA
CATCGATCACTCTGGAGGATACGTTGCTCAATTCAACATTTCTTGGGATGAAGTAAATTATGATCCTGAA
GGTAACGAAATTGTTCAACATAAAAACTGGAGCGAAAACAATAAAAGCAAGCTAGCTCATTTCACATCGT
CCATCTATTTGCCAGGTAACGCGAGAAATATTAATGTTTACGCTAAAGAATGCACTGGTTTAGCTTGGGA
ATGGTGGAGAACGGTAATTGATGACCGGAACTTACCACTTGTGAAAAATAGAAATATCTCCATCTGGGGC
ACCACGCTTTATCCGAAATATAGTAATAAAGTAGATAATCCAATCGAATA
-
Protein Sequence : Show Sequence
>NP_463733.1 listeriolysin O precursor [Listeria monocytogenes EGD-e]
MKKIMLVFITLILVSLPIAQQTEAKDASAFNKENSISSMAPPASPPASPKTPIEKKHADEIDKYIQGLDY
NKNNVLVYHGDAVTNVPPRKGYKDGNEYIVVEKKKKSINQNNADIQVVNAISSLTYPGALVKANSELVEN
QPDVLPVKRDSLTLSIDLPGMTNQDNKIVVKNATKSNVNNAVNTLVERWNEKYAQAYPNVSAKIDYDDEM
AYSESQLIAKFGTAFKAVNNSLNVNFGAISEGKMQEEVISFKQIYYNVNVNEPTRPSRFFGKAVTKEQLQ
ALGVNAENPPAYISSVAYGRQVYLKLSTNSHSTKVKAAFDAAVSGKSVSGDVELTNIIKNSSFKAVIYGG
SAKDEVQIIDGNLGDLRDILKKGATFNRETPGVPIAYTTNFLKDNELAVIKNNSEYIETTSKAYTDGKIN
IDHSGGYVAQFNISWDEVNYDPEGNEIVQHKNWSENNKSKLAHFTSSIYLPGNARNINVYAKECTGLAWE
WWRTVIDDRNLPLVKNRNISIWGTTLYPKYSNKVDNPIE
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
In an attempt to generate a subunit vaccine in this experimental disease model, eukaryotic plasmid DNA expression vectors containing genes encoding either the wild-type or modified forms of recombinant listeriolysin O (hly) were generated and used for genetic vaccination of naive mice. Results of these studies indicate that the intramuscular immunization of mice with specifically designed plasmid DNA constructs encoding recombinant forms of listeriolysin O (hly) stimulates peptide-specific CD8+ immune T cells that exhibit in vitro cytotoxic activity. More importantly, such immunization can provide protective immunity against a subsequent challenge with viable L. monocytogenes (Cornell et al., 1999).
- Related Vaccine(s):
L. monocytogenes DNA Vaccine encoding hly
|
|
2. iap
|
-
Gene Name :
iap
-
Sequence Strain (Species/Organism) :
Listeria monocytogenes strain L2K3-549C1
-
VO ID :
VO_0012404
-
NCBI Protein GI :
215422507
-
Other Database IDs :
CDD:333284
-
Taxonomy ID :
1639
-
Gene Strand (Orientation) :
?
-
Protein Name :
p60
-
Protein pI :
10.49
-
Protein Weight :
13324.02
-
Protein Length :
182
-
Protein Note :
Cell wall-associated hydrolase, NlpC family [Cell wall/membrane/envelope biogenesis]; cl28464
-
Protein Sequence : Show Sequence
>BAG85375.1 p60, partial [Listeria monocytogenes]
MSWNNLSSSSIYVGQKLAIKQTANTVTPKAEVKKEAPAAEKQAAPVVKENTNTNTNTTTEKKETTQQQTA
PKAPTEAAKPAPAPSTNTNANKTNTNTNNTNTSTPSKNTNTNTNSNTNTNSNTNANQGS
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Mice immunized with p60 encapsulated in liposomes launched a strong CTL response and were protected against a subsequent challenge with L. monocytogenes (Grenningloh et al., 2008).
- Related Vaccine(s):
L. monocytogenes p60 Protein Vaccine
|
| II. References |
1. Cornell et al., 1999: Cornell KA, Bouwer HG, Hinrichs DJ, Barry RA. Genetic immunization of mice against Listeria monocytogenes using plasmid DNA encoding listeriolysin O. Journal of immunology (Baltimore, Md. : 1950). 1999; 163(1); 322-329. [PubMed: 10384131].
2. Grenningloh et al., 2008: Grenningloh R, Darj A, Bauer H, zur Lage S, Chakraborty T, Jacobs T, Weiss S. Liposome-encapsulated antigens induce a protective CTL response against Listeria monocytogenes independent of CD4+ T cell help. Scandinavian journal of immunology. 2008; 67(6); 594-602. [PubMed: 18433404].
|
|
