VIOLIN: Vaccine Investigation and Online Information Network

KLK3

General Information

Protegen ID

717

Sequence Strain (Species/Organism)

Homo sapiens

Taxonomy ID

9606

Molecule Role

Protective antigen

Molecule Role Annotation

Plasmid expressing human PSA gene (phPSA) was delivered in vivo by intra-muscular electroporation, to induce effective anti-tumour immune responses against prostate antigen expressing tumours. Groups of male C57 BL/6 mice received intra-muscular injections of phPSA plasmid. One week after last vaccination, the mice were challenged subcutaneously with TRAMPC1/hPSA (prostate cancer cell line stably expressing human PSA) and tumour growth was monitored. The phPSA vaccine therapy significantly delayed the appearance of tumours and resulted in prolonged survival of the animals (Ahmad et al., 2010).

Related Vaccines(s)

APC8015F , Fowlpox Virus Vaccine , Fowlpox-PSA-TRICOM Vaccine , GVAX Cancer Vaccine , MVA-PSA/PAP Prostate Cancer Vaccine , Prostate cancer DNA vaccine psig-3P-Fc encoding 3P(hPSM, hPAP, or hPSA)-Fc fusion protein , Prostate Tumor Antigen-activated Autologous Dendritic Cell Vaccine , PROSTVAC-Contaminated W/ BVDV , PSA Prostate Cancer Vaccine , PSA RNA-Pulsed Dendritic Cell Vaccine , PSA-OP Peptide Vaccine , PSA:154-163(155L) Peptide Vaccine , PSMA/TARP Peptide Vaccine , pVAXrcPSAv53l DNA Vaccine , Recombinant Fowlpox-Prostate Specific Antigen Vaccine , Recombinant Fowlpox-PSA(L155)/TRICOM Vaccine , Recombinant Vaccinia DF3/MUC1 Vaccine , Recombinant Vaccinia PSA Vaccine , Recombinant Vaccinia-Prostate Specific Antigen Vaccine , Recombinant Vaccinia-PSA/PSMA Vaccine , rF-PSA/PSMA/TRICOM Vaccine , Rilimogene-galvacirepvec Vaccine

References

Ahmad et al., 2010: Ahmad S, Casey G, Sweeney P, Tangney M, O'Sullivan GC. Optimised electroporation mediated DNA vaccination for treatment of prostate cancer. Genetic vaccines and therapy. 2010; 8(1); 1. [PubMed: 20181099].

Gene Information

Gene Name

KLK3

NCBI Gene ID

354

Genbank Accession

AC011523

Chromosome No

Gene Starting Position

50854914

Gene Ending Position

50860763

DNA Sequence

>NC_000019.10:50854914-50860763 Homo sapiens chromosome 19, GRCh38.p12 Primary Assembly
CAGCCCCAAGCTTACCACCTGCACCCGGAGAGCTGTGTCACCATGTGGGTCCCGGTTGTCTTCCTCACCC
TGTCCGTGACGTGGATTGGTGAGAGGGGCCATGGTTGGGGGGATGCAGGAGAGGGAGCCAGCCCTGACTG
TCAAGCTGAGGCTCTTTCCCCCCCAACCCAGCACCCCAGCCCAGACAGGGAGCTGGGCTCTTTTCTGTCT
CTCCCAGCCCCACTCCAAGCCCATACCCCCAGCCCCTCCATATTGCAACAGTCCTCACTCCCACACCAGG
TCCCCGCTCCCTCCCACTTACCCCAGAACTTTCTCCCCATTGCCCAGCCAGCTCCCTGCTCCCAGCTGCT
TTACTAAAGGGGAAGTTCCTGGGCATCTCCGTGTTTCTCTTTGTGGGGCTCAAAACCTCCAAGGACCTCT
CTCAATGCCATTGGTTCCTTGGACCGTATCACTGGTCCACCTCCTGAGCCCCTCAATCCTATCACAGTCT
ACTGACTTTTCCCATTCAGCTGTGAGTGCCCAACCCTATCCCAGAGACCTTGATGCTTGGCCTCCCAATC
TTGCCCTAGGATACCCAGATGCCAACCAGACACCTCCTTCTTCCTAGCCAGGCTATCTGGCCTGAGACAA
CAAATGGGTCCCTCAGTCTGGCAATGGGACTCTGAGAACTCCTCATTCCCTGACTCTTAGCCCCAGACTC
TTCATTCAGTGGCCCACATTTTCCTTAGGAAAAACATGAGCATCCCCAGCCACAACTGCCAGCTCTCTGA
TTCCCCAAATCTGCATCCTTTTCAAAACCTAAAAACAAAAAGAAAAACAAATAAAACAAAACCAACTCAG
ACCAGAACTGTTTTCTCAACCTGGGACTTCCTAAACTTTCCAAAACCTTCCTCTTCCAGCAACTGAACCT
CGCCATAAGGCACTTATCCCTGGTTCCTAGCACCGCTTATCCCCTCAGAATCCACAACTTGTACCAAGTT
TCCCTTCTCCCAGTCCAAGACCCCAAATCACCACAAAGGACCCAATCCCCAGACTCAAGATATGGTCTGG
GCGCTGTCTTGTGTCTCCTACCCTGATCCCTGGGTTCAACTCTGCTCCCAGAGCATGAAGCCTCTCCACC
AGCACCAGCCACCAACCTGCAAACCTAGGGAAGATTGACAGAATTCCCAGCCTTTCCCAGCTCCCCCTGC
CCATGTCCCAGGACTCCCAGCCTTGGTTCTCTGCCCCCGTGTCTTTTCAAACCCACATCCTAAATCCATC
TCCTATCCGAGTCCCCCAGTTCCTCCTGTCAACCCTGATTCCCCTGATCTAGCACCCCCTCTGCAGGTGC
TGCACCCCTCATCCTGTCTCGGATTGTGGGAGGCTGGGAGTGCGAGAAGCATTCCCAACCCTGGCAGGTG
CTTGTGGCCTCTCGTGGCAGGGCAGTCTGCGGCGGTGTTCTGGTGCACCCCCAGTGGGTCCTCACAGCTG
CCCACTGCATCAGGAAGTGAGTAGGGGCCTGGGGTCTGGGGAGCAGGTGTCTGTGTCCCAGAGGAATAAC
AGCTGGGCATTTTCCCCAGGATAACCTCTAAGGCCAGCCTTGGGACTGGGGGAGAGAGGGAAAGTTCTGG
TTCAGGTCACATGGGGAGGCAGGGTTGGGGCTGGACCACCCTCCCCATGGCTGCCTGGGTCTCCATCTGT
GTTCCTCTATGTCTCTTTGTGTCGCTTTCATTATGTCTCTTGGTAACTGGCTTCGGTTGTGTCTCTCCGT
GTGACTATTTTGTTCTCTCTCTCCCTCTCTTCTCTGTCTTCAGTCTCCATATCTCCCCCTCTCTCTGTCC
TTCTCTGGTCCCTCTCTAGCCAGTGTGTCTCACCCTGTATCTCTCTGCCAGGCTCTGTCTCTCGGTCTCT
GTCTCACCTGTGCCTTCTCCCTACTGAGCACACGCATGGGATGGGCCTGGGGGGACCCTGAGAAAAGGAA
GGGCTTTGGCTGGGCGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGTAGATCA
CCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACTGGTGAAACCCCATCTCTACTAAAAATACAAAAAA
TTAGCCAGGCGTGGTGGCGCATGCCTGTAGTCCCAGCTACTCAGGAGGCTGAGGGAGGAGAATTGCTTGA
ACCTGGGAGGTGGAGGTTGCAGTGAGCCGAGACCGTGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGA
CTCCGCCTCAAAAAAAAAAAAAAAAAAAAAGAAAAGAAAAGAAAAGAAAAGGAAGTGTTTTATCCCTGAT
GTGTGTGGGTATGAGGGTATGAGAGGGCCCCTCTCACTCCATTCCTTCTCCAGGACATCCCTCCACTCTT
GGGAGACACAGAGAAGGGCTGGTTCCAGCTGGAGCTGGGAGGGGCAATTGAGGGAGGAGGAAGGAGAAGG
GGGAAGGAAAACAGGGTATGGGGGAAAGGACCCTGGGGAGCGAAGTGGAGGATACAACCTTGGGCCTGCA
GGCCAGGCTACCTACCCACTTGGAAACCCACGCCAAAGCCGCATCTACAGCTGAGCCACTCTGAGGCCTC
CCCTCCCCAGCGGTCCCCACTCAGCTCCAAAGTCTCTCTCCCTTTTCTCTCCCACACTCTATCATCCCCC
GGATTCCTCTCTACTTGGTTCTCATTCTTCCTTTGACTTCCTGCTTCCCTTTCTCATTCATCTGTTTCTC
ACTTTCTGCCTGGTTTTGTTCTTCTCTCTCTCTTTCTCTGGCCCATGTCTGTTTCTCTATGTTTCTGTCT
TTTCTTTCTCATCCTGTGTATTTTCGGCTCACCTTGTTTGTCACTGTTCTCCCCTCTGCCCTTTCATTCT
CTCTGTCCTTTTACCCTCTTCCTTTTTCCCTTGGTTTCTCTCAGTTTCTGTATCTGCCCTTCACCCTCTC
ACACTGCTGTTTCCCAACTCGTTGTCTGTATTTTTGGCCTGAACTGTGTCTTCCCCAACCCTGTGTTTTT
CTCACTGTTTCTTTTTCTCTTTTGGAGCCTCCTCCTTGCTCCTCTGTCCCTTCTCTCTTTCCTTATCATC
CTCGCTCCTCATTCCTGCGTCTGCTTCCTCCCCAGCAAAAGCGTGATCTTGCTGGGTCGGCACAGCCTGT
TTCATCCTGAAGACACAGGCCAGGTATTTCAGGTCAGCCACAGCTTCCCACACCCGCTCTACGATATGAG
CCTCCTGAAGAATCGATTCCTCAGGCCAGGTGATGACTCCAGCCACGACCTCATGCTGCTCCGCCTGTCA
GAGCCTGCCGAGCTCACGGATGCTGTGAAGGTCATGGACCTGCCCACCCAGGAGCCAGCACTGGGGACCA
CCTGCTACGCCTCAGGCTGGGGCAGCATTGAACCAGAGGAGTGTACGCCTGGGCCAGATGGTGCAGCCGG
GAGCCCAGATGCCTGGGTCTGAGGGAGGAGGGGACAGGACTCCTAGGTCTGAGGGAGGAGGGCCAAGGAA
CCAGGTGGGGTCCAGCCCACAACAGTGTTTTTGCCTGGCCCGTAGTCTTGACCCCAAAGAAACTTCAGTG
TGTGGACCTCCATGTTATTTCCAATGACGTGTGTGCGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATG
CTGTGTGCTGGACGCTGGACAGGGGGCAAAAGCACCTGCTCGGTGAGTCATCCCTACTCCCAAGATCTTG
AGGGGAAAGGTGAGTGGGGACCTTAATTCTGGGCTGGGGTCTAGAAGCCAACAAGGCGTCTGCCTCCCCT
GCTCCCCAGCTGTAGCCATGCCACCTCCCCGTGTCTCATCTCATTCCCTCCTTCCCTCTTCTTTGACTCC
CTCAAGGCAATAGGTTATTCTTACAGCACAACTCATCTGTTCCTGCGTTCAGCACACGGTTACTAGGCAC
CTGCTATGCACCCAGCACTGCCCTAGAGCCTGGGACATAGCAGTGAACAGACAGAGAGCAGCCCCTCCCT
TCTGTAGCCCCCAAGCCAGTGAGGGGCACAGGCAGGAACAGGGACCACAACACAGAAAAGCTGGAGGGTG
TCAGGAGGTGATCAGGCTCTCGGGGAGGGAGAAGGGGTGGGGAGTGTGACTGGGAGGAGACATCCTGCAG
AAGGCGGGAGTGAGCAAACACCTGCCGCAGGGGAGGGGAGGGCCCTGCGGCACCTGGGGGAGCAGAGGGA
ACAGCATCTGGCCAGGCCTGGGAGGAGGGGCCTAGAGGGCGTCAGGAGCAGAGAGGAGGTTGCCTGGCTG
GAGTGAAGGATCGGGGCAGGGTGCGAGAGGGAAGAAAGGACCCCTCCTGCAGGGCCTCACCTGGGCCACA
GGAGGACACTGCTTTTCCTCTGAGGAGTCAGGAACTGTGGATGGTGCTGGACAGAAGCAGGACAGGGCCT
GGCTCAGGTGTCCAGAGGCTGCCGCTGGCCTCCCTATGGGATCAGACTGCAGGGAGGGAGGGCAGCAGGG
ATGTGGAGGGAGTGATGATGGGGCTGACCTGGGGGTGGCTCCAGGCATTGTCCCCACCTGGGCCCTTACC
CAGCCTCCCTCACAGGCTCCTGGCCCTCAGTCTCTCCCCTCCACTCCATTCTCCACCTACCCACAGTGGG
TCATTCTGATCACCGAACTGACCATGCCAGCCCTGCCGATGGTCCTCCATGGCTCCCTAGTGCCCTGGAG
AGGAGGTGTCTAGTCAGAGAGTAGTCCTGGAAGGTGGCCTCTGTGAGGAGCCACGGGGACAGCATCCTGC
AGATGGTCCTGGCCCTTGTCCCACCGACCTGTCTACAAGGACTGTCCTCGTGGACCCTCCCCTCTGCACA
GGAGCTGGACCCTGAAGTCCCTTCCCCACCGGCCAGGACTGGAGCCCCTACCCCTCTGTTGGAATCCCTG
CCCACCTTCTTCTGGAAGTCGGCTCTGGAGACATTTCTCTCTTCTTCCAAAGCTGGGAACTGCTATCTGT
TATCTGCCTGTCCAGGTCTGAAAGATAGGATTGCCCAGGCAGAAACTGGGACTGACCTATCTCACTCTCT
CCCTGCTTTTACCCTTAGGGTGATTCTGGGGGCCCACTTGTCTGTAATGGTGTGCTTCAAGGTATCACGT
CATGGGGCAGTGAACCATGTGCCCTGCCCGAAAGGCCTTCCCTGTACACCAAGGTGGTGCATTACCGGAA
GTGGATCAAGGACACCATCGTGGCCAACCCCTGAGCACCCCTATCAACCCCCTATTGTAGTAAACTTGGA
ACCTTGGAAATGACCAGGCCAAGACTCAAGCCTCCCCAGTTCTACTGACCTTTGTCCTTAGGTGTGAGGT
CCAGGGTTGCTAGGAAAAGAAATCAGCAGACACAGGTGTAGACCAGAGTGTTTCTTAAATGGTGTAATTT
TGTCCTCTCTGTGTCCTGGGGAATACTGGCCATGCCTGGAGACATATCACTCAATTTCTCTGAGGACACA
GATAGGATGGGGTGTCTGTGTTATTTGTGGGGTACAGAGATGAAAGAGGGGTGGGATCCACACTGAGAGA
GTGGAGAGTGACATGTGCTGGACACTGTCCATGAAGCACTGAGCAGAAGCTGGAGGCACAACGCACCAGA
CACTCACAGCAAGGATGGAGCTGAAAACATAACCCACTCTGTCCTGGAGGCACTGGGAAGCCTAGAGAAG
GCTGTGAGCCAAGGAGGGAGGGTCTTCCTTTGGCATGGGATGGGGATGAAGTAAGGAGAGGGACTGGACC
CCCTGGAAGCTGATTCACTATGGGGGGAGGTGTATTGAAGTCCTCCAGACAACCCTCAGATTTGATGATT
TCCTAGTAGAACTCACAGAAATAAAGAGCTGTTATACTGT

Protein Information

Protein Name

transcript variant 3

NCBI Protein GI

71834853

Protein Accession

NP_001025218

Protein pI

7.55

Protein Weight

23836.77

Protein Length

238

Protein Note

Also known as APS; PSA; hK3; KLK2A1

Protein Sequence

>NP_001025218.1 prostate-specific antigen isoform 3 preproprotein [Homo sapiens]
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNK
SVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMD
LPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCS
WVILITELTMPALPMVLHGSLVPWRGGV

Epitope Information

IEDB Linear Epitope