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Actinobacillus pleuropneumoniae HS93Tox-/pIG-T1K vectored vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004607
- Type: Recombinant vector vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mice
- Vector:
- Preparation: The HS93Tox- mutant of the serovar 7 strain of Actinobacillus pleuropneumoniae lacks the genes encoding the toxin ApxA and the post-translational activating protein ApxC, but still has genes required for secretion, making it a good vector. A plasmid vector was created to express the ApxA gene inside the HS93Tox- mutant of A. pleuropneumoniae, so that the bacterium acts as a vector for its own natural toxin (Prideaux et al., 1998).
- Immunization Route: Intramuscular injection (i.m.)
- Virulence: Because the toxin has been attenuated, this greatly decreases the potential virulence of the vaccine.
- Description: The HS93Tox-/pIG-T1K vector strain, using an Actinobacillus pleuropneumoniae to express the AxpA gene, was shown to protect mice against Actinobacillus pleuropneumoniae challenges (Prideaux et al., 1998). ApxA gene encodes an A. pleuropneumoniae structural toxin (Prideaux et al., 1998).
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| References |
Prideaux et al., 1998: Prideaux CT, Pierce L, Krywult J, Hodgson AL. Protection of mice against challenge with homologous and heterologous serovars of Actinobacillus pleuropneumoniae after live vaccination. Current microbiology. 1998; 37(5); 324-332. [PubMed: 9767712].
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Aeromonas hydrophila LSA34 vector vaccine WED/pUTDgap |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004606
- Type: Recombinant vector vaccine
- Status: Research
- Antigen: The protective antigen GAPDH from the fish pathogen A. hydrophila, encoded by the gene gapA34 (Mu et al., 2011).
- Vector:
- Vactor Name:
- VO ID: VO_0001032
- Description: Because E. tarda is commonly a fish pathogen, it is useful as a vector for other pathogens in fish. It has been shown to elicit strong immune responses (Mu et al., 2011).
- Preparation: The gapA34 gene was introduced into P(dps) and transformed into live, attenuated E. tarda strain EIB202 (Mu et al., 2011).
- Immunization Route: Intramuscular injection (i.m.)
- Description: A. hydrophila is a common fish pathogen, so in order to create a vaccine against this pathogen, the gapA34 gene of A. hydrophila was expressed in the bacterial vector E. tarda strain EIB202, and it was shown to increase survival of vaccinated fish (Mu et al., 2011).
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| References |
Mu et al., 2011: Mu W, Guan L, Yan Y, Liu Q, Zhang Y. A novel in vivo inducible expression system in Edwardsiella tarda for potential application in bacterial polyvalence vaccine. Fish & shellfish immunology. 2011; 31(6); 1097-1105. [PubMed: 21964456].
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BL21(DE3)/pUTa-E + pET28a-FT |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004804
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Zebrafish
- Vector:
- Preparation: Cell-penetrating peptides (CPPs) are short cationic/amphipathic peptides which facilitate cellular uptake of various molecular cargoes and therefore have great potentials in vector vaccine design. Among the tested CPPs, TAT showed an excellent capability to deliver the cargo protein EGFP into cytoplasm (Ma et al., 2014). In order to establish an efficient antigen delivery system in Escherichia coli, the EGFP-TAT synthesis circuit was combined with an in vivo inducible lysis circuit PviuA-E in E. coli to form an integrated antigen delivery system, the resultant E. coli was proved to be able to lyse upon the induction of a mimic in vivo signal and thus release intracellular EGFP-TAT intensively, which were assumed to undergo a more efficient intracellular delivery by CPP to evoke protective immune responses. Based on the established antigen delivery system, the protective antigen gene flgD from an invasive intracellular fish pathogen Edwardsiella tarda EIB202, was applied to establish an E. coli recombinant vector vaccine (Ma et al., 2014).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
Ma et al., 2014: Ma J, Xu J, Guan L, Hu T, Liu Q, Xiao J, Zhang Y. Cell-penetrating peptides mediated protein cross-membrane delivery and its use in bacterial vector vaccine. Fish & shellfish immunology. 2014; 39(1); 8-16. [PubMed: 24746937].
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Bordetella pertussis BPZE vectored vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004608
- Type: Recombinant vector vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- Antigen: The pertussis toxin gene, which encodes a highly virulent toxin (Mielcarek et al., 2006).
- Vector:
- Immunization Route: intranasal immunization
- Virulence: The pertussis toxin itself if extremely virulent, but after thorough and careful attenuation, the virulence is basically gone (Mielcarek et al., 2006).
- Description: This is an intranasal vaccine because pathogenic B. pertussis colonizes the respiratory tract of humans. The vaccine itself has been shown to only need one inoculation in order to elicit protection, making it a good candidate to be a vaccine vector for other diseases (Mielcarek et al., 2006).
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| References |
Mielcarek et al., 2006: Mielcarek N, Debrie AS, Raze D, Quatannens J, Engle J, Goldman WE, Locht C. Attenuated Bordetella pertussis: new live vaccines for intranasal immunisation. Vaccine. 2006; 24 Suppl 2; S2-54-5. [PubMed: 16823926].
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CVD 103-HgR- REPEC |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004721
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Vector:
- Preparation: Rabbit Enteropathogenic E. coli (REPEC), a member of the AEEC family. The C-terminal portion of intimin was delivered by the attenuated Vibrio cholerae vaccine strain CVD 103-HgR. To export intimin, a fusion was engineered with ClyA, a secreted protein from Salmonella enterica serovar Typhi (Keller et al., 2010).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
Keller et al., 2010: Keller R, Hilton TD, Rios H, Boedeker EC, Kaper JB. Development of a live oral attaching and effacing Escherichia coli vaccine candidate using Vibrio cholerae CVD 103-HgR as antigen vector. Microbial pathogenesis. 2010; 48(1); 1-8. [PubMed: 19878715].
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E. coli-CPPs (Edwardsiella tarda) |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004640
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Vector:
- Preparation: GFP-TAT synthesis circuit was combined with an in vivo inducible lysis circuit PviuA-E in E. coli to form an integrated antigen delivery system (Ma et al., 2014).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
Ma et al., 2014: Ma J, Xu J, Guan L, Hu T, Liu Q, Xiao J, Zhang Y. Cell-penetrating peptides mediated protein cross-membrane delivery and its use in bacterial vector vaccine. Fish & shellfish immunology. 2014; 39(1); 8-16. [PubMed: 24746937].
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HS93Tox-/pIG-T1K |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004637
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- apxIA
gene engineering:
- Type: Recombinant vector construction
- Description: HS93Tox-/pIG-T1K, expresses the Apx structural protein in a non-activated form (Prideaux et al., 1998).
- Detailed Gene Information: Click Here.
- Vector:
- Preparation: HS93Tox-/pIG-T1K, expresses the Apx structural protein in a non-activated form (Prideaux et al., 1998).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
Prideaux et al., 1998: Prideaux CT, Pierce L, Krywult J, Hodgson AL. Protection of mice against challenge with homologous and heterologous serovars of Actinobacillus pleuropneumoniae after live vaccination. Current microbiology. 1998; 37(5); 324-332. [PubMed: 9767712].
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M. S- H. pylori -opm26 |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004688
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Vector:
- Preparation: Recombinant Mycobacterium smegmatis expressing the H. pylori outer membrane protein 26-kilodalton (Omp26) antigen (Lü et al., 2011).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
Lü et al., 2011: Lü L, Zeng HQ, Wang PL, Shen W, Xiang TX, Mei ZC. Oral immunization with recombinant Mycobacterium smegmatis expressing the outer membrane protein 26-kilodalton antigen confers prophylactic protection against Helicobacter pylori infection. Clinical and vaccine immunology : CVI. 2011; 18(11); 1957-1961. [PubMed: 21900527].
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rAd-P97c |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004697
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- P97
gene engineering:
- Type: Recombinant vector construction
- Description: a P97 recombinant replication-defective adenovirus (rAdP97c) subunit vaccine (Okamba et al., 2010).
- Detailed Gene Information: Click Here.
- Vector:
- Preparation: P97 recombinant replication-defective adenovirus (rAdP97c) subunit vaccine (Okamba et al., 2010).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
Okamba et al., 2010: Okamba FR, Arella M, Music N, Jia JJ, Gottschalk M, Gagnon CA. Potential use of a recombinant replication-defective adenovirus vector carrying the C-terminal portion of the P97 adhesin protein as a vaccine against Mycoplasma hyopneumoniae in swine. Vaccine. 2010; 28(30); 4802-4809. [PubMed: 20472025].
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rBCG -Stx2B (Escherichia coli ) |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004657
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- StxB2
gene engineering:
- Type: Recombinant vector construction
- Description: A novel vaccine against Shiga toxin (Stx)-producing Escherichia coli (STEC) infection using a recombinant Mycobacterium bovis BCG (rBCG) system expressing the Stx2 B subunit (Stx2B) (Fujii et al., 2012).
- Detailed Gene Information: Click Here.
- Vector:
- Preparation: rBCG expressing the Stx2 B subunit (Stx2B) (Fujii et al., 2012).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
Fujii et al., 2012: Fujii J, Naito M, Yutsudo T, Matsumoto S, Heatherly DP, Yamada T, Kobayashi H, Yoshida S, Obrig T. Protection by a recombinant Mycobacterium bovis Bacillus Calmette-Guerin vaccine expressing Shiga toxin 2 B subunit against Shiga toxin-producing Escherichia coli in mice. Clinical and vaccine immunology : CVI. 2012; 19(12); 1932-1937. [PubMed: 23035176].
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RCN-F1-V (Yersinia pestis F1 and V) |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004692
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Vector:
- Preparation: Recombinant raccoon poxvirus (RCN) expressing plague antigens (fraction 1 [F1] and a truncated form of the V protein-V307) (Rocke et al., 2010).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
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RCN-IRES-tPA-YpF1( Yersinia pestis) |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004694
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Vector:
- Preparation: Translation enhancer (EMCV-IRES) in combination with a secretory (tPA) signal or secretory (tPA) and membrane anchoring (CHV-gG) signals on in vitro antigen expression of F1 antigen (Osorio et al., 2003).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
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rS.C-APP-ApxI/ApxII |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004785
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- apxIA
gene engineering:
- Type: Recombinant vector construction
- Description: S. cerevisiae expressing either ApxI or ApxII (Shin et al., 2013).
- Detailed Gene Information: Click Here.
- apxIIA
gene engineering:
- Type: Recombinant vector construction
- Description: S. cerevisiae expressing either ApxI or ApxII (Shin et al., 2013).
- Detailed Gene Information: Click Here.
- Vector:
- Preparation: The surface-displayed ApxIIA#5 expressing S. cerevisiae and the ApxIA expressing S. cerevisiae (Shin et al., 2013).
- Immunization Route: Oral immunization
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| References |
Shin et al., 2013: Shin MK, Kang ML, Jung MH, Cha SB, Lee WJ, Kim JM, Kim DH, Yoo HS. Induction of protective immune responses against challenge of Actinobacillus pleuropneumoniae by oral administration with Saccharomyces cerevisiae expressing Apx toxins in pigs. Veterinary immunology and immunopathology. 2013; 151(1-2); 132-139. [PubMed: 23206402].
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rS.C-APP-ApxIIA |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004786
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- apxIIA
gene engineering:
- Type: Recombinant vector construction
- Description: A neutralizing epitope fragment of ApxIIA toxin was expressed on the cell surface of Saccharomyces cerevisiae (Kim et al., 2010).
- Detailed Gene Information: Click Here.
- Vector:
- Preparation: (Kim et al., 2010) A neutralizing epitope fragment of ApxIIA toxin was expressed and immobilized on the cell surface of Saccharomyces cerevisiae for efficient vaccine development.
- Immunization Route: Oral vaccination
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| References |
Kim et al., 2010: Kim JM, Jung DI, Eom YJ, Park SM, Yoo HS, Jang YS, Yang MS, Kim DH. Surface-displayed expression of a neutralizing epitope of ApxIIA exotoxin in Saccharomyces cerevisiae and oral administration of it for protective immune responses against challenge by Actinobacillus pleuropneumoniae. Bioscience, biotechnology, and biochemistry. 2010; 74(7); 1362-1367. [PubMed: 20622458].
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rVCG- Chlamydia-cholera |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004720
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Vector:
- Preparation: A multisubunit vaccine candidate co-expressing the serovar D-derived Porin B and polymorphic membrane protein-D proteins of Chlamydia trachomatis (Eko et al., 2011; Chen et al., 1998).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
Chen et al., 1998: Chen I, Finn TM, Yanqing L, Guoming Q, Rappuoli R, Pizza M. A recombinant live attenuated strain of Vibrio cholerae induces immunity against tetanus toxin and Bordetella pertussis tracheal colonization factor. Infection and immunity. 1998; 66(4); 1648-1653. [PubMed: 9529093].
Eko et al., 2011: Eko FO, Okenu DN, Singh UP, He Q, Black C, Igietseme JU. Evaluation of a broadly protective Chlamydia-cholera combination vaccine candidate. Vaccine. 2011; 29(21); 3802-3810. [PubMed: 21421002].
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rVCG- MOMP/HSV-2-gD |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004722
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Vector:
- Preparation: A bivalent combination vaccine formulation comprising rVCG expressing chlamydial MOMP and HSV-2 glycoprotein D (Macmillan et al., 2007).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
Macmillan et al., 2007: Macmillan L, Ifere GO, He Q, Igietseme JU, Kellar KL, Okenu DM, Eko FO. A recombinant multivalent combination vaccine protects against Chlamydia and genital herpes. FEMS immunology and medical microbiology. 2007; 49(1); 46-55. [PubMed: 17094789].
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S. gordonii-PT |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004670
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Vector:
- Preparation: (Lee et al., 1999)
- Immunization Route: Intramuscular injection (i.m.)
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| References |
Lee et al., 1999: Lee SF, March RJ, Halperin SA, Faulkner G, Gao L. Surface expression of a protective recombinant pertussis toxin S1 subunit fragment in Streptococcus gordonii. Infection and immunity. 1999; 67(3); 1511-1516. [PubMed: 10024603].
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SL3261- UreA and UreB |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004675
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- ureB
gene engineering:
- Type: Recombinant vector construction
- Description: UreA and UreB, two structural subunits of the active enzyme, were expressed in the attenuated Salmonella typhimurium live vaccine SL3261 strain (Gómez-Duarte et al., 1998).
- Detailed Gene Information: Click Here.
- Vector:
- Immunization Route: Intramuscular injection (i.m.)
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| References |
Gómez-Duarte et al., 1998: Gómez-Duarte OG, Lucas B, Yan ZX, Panthel K, Haas R, Meyer TF. Protection of mice against gastric colonization by Helicobacter pylori by single oral dose immunization with attenuated Salmonella typhimurium producing urease subunits A and B. Vaccine. 1998; 16(5); 460-471. [PubMed: 9491500].
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SL3261-P. aeruginosa serogroup O11 O antigen |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004674
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Vector:
- Preparation: Attenuated Salmonella enterica serovar Typhimurium SL3261 expressing P. aeruginosa serogroup O11 O antigen (DiGiandomenico et al., 2004).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
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Ty21a-O-Ps (Shigella dysenteriae ) |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004703
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Vector:
- Preparation: A tandemly-linked rfb-rfp gene cassette was cloned into low copy plasmid pGB2 to create pSd1 (Xu et al., 2007).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
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Typhi strain Ty21a-LPS-Shigella |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004701
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Vector:
- Preparation: Ty21a-Ss simultaneously expresses both homologous Ty21a and heterologous S. sonnei O-antigens (Dharmasena et al., 2013).
- Immunization Route: Intramuscular injection (i.m.)
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| References |
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VC-TetC/Tcf |
| Vaccine Information |
- Product Name: VC expressing B. pertussis TetC/Tcf
- Vaccine Ontology ID: VO_0004781
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- TcfA
gene engineering:
- Type: Recombinant protein preparation
- Description: An attenuated strain of Vibrio cholerae was used as a carrier for the expression of heterologous antigens such as fragment C from tetanus toxin (TetC) and tracheal colonization factor from Bordetella pertussis (Tcf) (Chen et al., 1998).
- Detailed Gene Information: Click Here.
- Vector:
- Preparation: An attenuated strain of Vibrio cholerae was used as a carrier for the expression of heterologous antigens such as fragment C from tetanus toxin (TetC) and tracheal colonization factor from Bordetella pertussis (Tcf) (Chen et al., 1998).
- Immunization Route: intranasal immunization
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| References |
Chen et al., 1998: Chen I, Finn TM, Yanqing L, Guoming Q, Rappuoli R, Pizza M. A recombinant live attenuated strain of Vibrio cholerae induces immunity against tetanus toxin and Bordetella pertussis tracheal colonization factor. Infection and immunity. 1998; 66(4); 1648-1653. [PubMed: 9529093].
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