Immunoliposomes Containing Antibodies to Costimulatory Molecules |
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Vaxjo ID |
94 |
|
Vaccine Adjuvant Name |
Immunoliposomes Containing Antibodies to Costimulatory Molecules |
|
Alternative Names |
Immunoliposomes prepared from Dehydration-Rehyrdation Vesicles (DRVs) |
|
Adjuvant VO ID |
VO_0001343
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Stage of Development |
Research |
|
Structure |
This is a DRV composed of phosphotidylcholine/ cholesterol/ biotinylate d-phospotidylethanolamine (PC/ CH/ PEB) in an molar ration of 5: 5: 1. The DRVs were created from the dried film by sequentially adding a small volume of water followed by 30 seconds of vortexing. Antigen is added to the water suspension of DRV followed by repeated vortexing and lyophylization of the liposome suspension. The DRVs were hydrated with water, treated with avidin dissolved in water and washed three times in PBS prior to addition of the blotinylated-mAb (Vogel and Powell, 1995). |
|
Appearance |
Cloudy suspension when in solution or white powder when dried. |
|
Storage |
Prepare immediately before use, but may be stored at 4°C (Vogel and Powell, 1995). |
|
Function |
Induces delayed-type hypersensitivity, but not humoral immunity (Vogel and Powell, 1995). |
| Related Vaccine(s) |
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| References |
Vogel and Powell, 1995: Vogel FR, Powell MF. A compendium of vaccine adjuvants and excipients. Pharmaceutical biotechnology. 1995; 6; 141-228. [PubMed: 7551218].
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