VIOLIN: Vaccine Investigation and Online Information Network

Nipah virus

Table of Contents

Protective Antigens
1. F fusion protein
2. G glycoprotein

I. Vaccine Related Protective Antigens

Gene Name : F fusion protein
Sequence Strain (Species/Organism) : Nipah virus
VO ID : VO_0011314
NCBI Nucleotide GI : 8164021
NCBI Protein GI : 8164022
Protein Accession : AAF73956.1
Other Database IDs : CDD:306910
Taxonomy ID : 121791
Gene Strand (Orientation) : ?
Protein Name : fusion protein
Protein pI : 5.9
Protein Weight : 56616.49
Protein Length : 602
Protein Note : Fusion glycoprotein F0; pfam00523

DNA Sequence : Show Sequence

>gi|8164021|gb|AF238466.1| Nipah virus fusion protein mRNA, complete cds
AGGAGCCAAGCTCTTGCCTCGTTCAGAAGGTTAAACAAGCATTCTTACCATTGGATCAACAAAAGGATTG
GTTTTATCGTCTAAGAAATTTATTGAAAGGCAAAGAAATTCCTGGTTTTATGTTGAATGAGGTGTATCAA
ACTAAGGAGACCTTCTAACAGCCAGGTCATAGGAATATAAATAAAAATAAGAATAAAATTGATTCCATCG
GAAGATTCATTTCAAGAAGTGATCAAATCAAAGCGGTTGGCAGACCTACCAATCATATACCACAAGACTC
GACAATGGTAGTTATACTTGACAAGAGATGTTATTGTAATCTTTTAATATTGATTTTGATGATCTCGGAG
TGTAGTGTTGGGATTCTACATTATGAGAAATTGAGTAAAATTGGACTTGTCAAAGGAGTAACAAGAAAAT
ACAAGATTAAAAGCAATCCTCTCACAAAAGACATTGTTATAAAAATGATTCCGAATGTGTCGAACATGTC
TCAGTGCACAGGGAGTGTCATGGAAAATTATAAAACACGATTAAACGGTATCTTAACACCTATAAAGGGA
GCGTTAGAGATCTACAAAAACAACACTCATGACCTTGTCGGTGATGTGAGATTAGCCGGAGTTATAATGG
CAGGAGTTGCTATTGGGATTGCAACCGCAGCTCAAATCACTGCAGGTGTAGCACTATATGAGGCAATGAA
GAATGCTGACAACATCAACAAACTCAAAAGCAGCATTGAATCAACTAATGAAGCTGTCGTTAAACTTCAA
GAGACTGCAGAAAAGACAGTCTATGTGCTGACTGCTCTACAGGATTACATTAATACTAATTTAGTACCGA
CAATTGACAAGATAAGCTGCAAACAGACAGAACTCTCACTAGATCTGGCATTATCAAAGTACCTCTCTGA
TTTGCTTTTTGTATTTGGCCCCAACCTTCAAGACCCAGTTTCTAATTCAATGACTATACAGGCTATATCT
CAGGCATTCGGTGGAAATTATGAAACACTGCTAAGAACATTGGGTTACGCTACAGAAGACTTTGATGATC
TTCTAGAAAGTGACAGCATAACAGGTCAAATCATCTATGTTGATCTAAGTAGCTACTATATAATTGTCAG
GGTTTATTTTCCTATTCTGACTGAAATTCAACAGGCCTATATCCAAGAGTTGTTACCAGTGAGCTTCAAC
AATGATAATTCAGAATGGATCAGTATTGTCCCAAATTTCATATTGGTAAGGAATACATTAATATCAAATA
TAGAGATTGGATTTTGCCTAATTACAAAGAGGAGCGTGATCTGCAACCAAGATTATGCCACACCTATGAC
CAACAACATGAGAGAATGTTTAACGGGATCGACTGAGAAGTGTCCTCGAGAGCTGGTTGTTTCATCACAT
GTTCCCAGATTTGCACTATCTAACGGGGTTCTGTTTGCCAATTGCATAAGTGTTACATGTCAGTGTCAAA
CAACAGGCAGGGCAATCTCACAATCAGGAGAACAAACTCTGCTGATGATTGACAACACCACCTGTCCTAC
AGCCGTACTCGGTAATGTGATTATCAGCTTAGGGAAATATCTGGGGTCAGTAAATTATAATTCTGAAGGC
ATTGCTATCGGTCCTCCAGTCTTTACAGATAAAGTTGATATATCAAGTCAGATATCCAGCATGAATCAGT
CCTTACAACAGTCTAAGGACTATATCAAAGAGGCTCAACGACTCCTTGATACTGTTAATCCATCATTAAT
AAGCATGTTGTCTATGATCATACTGTATGTATTATCGATCGCATCGTTGTGTATAGGGTTGATTACATTT
ATCAGTTTTATCATTGTTGAGAAAAAGAGAAACACCTACAGCAGATTAGAGGATAGGAGAGTCAGACCTA
CAAGCAGTGGGGATCTCTACTACATTGGGACATAGTGTATTCAGATTGATGAAATTATGTTAGAGAAATC
AGAAAACTTCTGACTTTCAGAAATGGATTGTATACAATTAGTTAGATCATCCTGAATAATCGAGGTGAGA
ACATTGCAACTATAAAATCAGATCATGTAAATAGTTGTAAAAAATTAAAAGCTTCTTTTAATTCTTTTGA
ACAATAATTTAATTAATATATAACATATTCTCTCACACGAGCGCTAACCTATACACTCTCTACTAATATT
TTATACTCATAATTAATGATATAATGACAAATAAGGATTCAAATTGGATTATGATATAGTTTCATACTAC
AATAGCATTTCGACCAAGAAAATATCCTTACAATTATACAATGTACTTAACCGTGAATATGTAATTGATA
ATTTCCCTTTAGAAATTTAATAAAAAA

Protein Sequence : Show Sequence

>AAF73956.1 fusion protein [Nipah henipavirus]
MVVILDKRCYCNLLILILMISECSVGILHYEKLSKIGLVKGVTRKYKIKSNPLTKDIVIKMIPNVSNMSQ
CTGSVMENYKTRLNGILTPIKGALEIYKNNTHDLVGDVRLAGVIMAGVAIGIATAAQITAGVALYEAMKN
ADNINKLKSSIESTNEAVVKLQETAEKTVYVLTALQDYINTNLVPTIDKISCKQTELSLDLALSKYLSDL
LFVFGPNLQDPVSNSMTIQAISQAFGGNYETLLRTLGYATEDFDDLLESDSITGQIIYVDLSSYYIIVRV
YFPILTEIQQAYIQELLPVSFNNDNSEWISIVPNFILVRNTLISNIEIGFCLITKRSVICNQDYATPMTN
NMRECLTGSTEKCPRELVVSSHVPRFALSNGVLFANCISVTCQCQTTGRAISQSGEQTLLMIDNTTCPTA
VLGNVIISLGKYLGSVNYNSEGIAIGPPVFTDKVDISSQISSMNQSLQQSKDYIKEAQRLLDTVNPSLIS
MLSMIILYVLSIASLCIGLITFISFIIVEKKRNTYSRLEDRRVRPTSSGDLYYIGT

Molecule Role : Protective antigen
Molecule Role Annotation : Hamsters were immunized subcutaneously with either 10^7 PFU of VV-NiV.G or VV-NiV.F expressing the G and F glycoproteins, respectively, or with the two combined. When the VV-NiV.F-vaccinated animals were challenged with Nipah virus 3 months after the last immunization (1,000 PFU/animal intraperitoneally), there was complete protection against mortality (Guillaume et al., 2004).
Related Vaccine(s): ALVAC-NiV-F , ALVAC-NiV-G , NIpah Virus G and F Proteins Subunit Vaccine

2. G glycoprotein

Gene Name : G glycoprotein
Sequence Strain (Species/Organism) : Nipah henipavirus
VO ID : VO_0011313
NCBI Gene ID : 920955
NCBI Protein GI : 13559814
Locus Tag : Nvgp5
Genbank Accession : AF212302
Protein Accession : NP_112027
3D structure: PDB ID : 2VWD
Taxonomy ID : 121791
Gene Starting Position : 8709
Gene Ending Position : 11254
Gene Strand (Orientation) : +
Protein Name : attachment glycoprotein
Protein pI : 8.36
Protein Weight : 62969.63
Protein Length : 602

DNA Sequence : Show Sequence

>NC_002728.1:8709-11254 Nipah virus, complete genome
TAGGACCCAGGTCCATAACTCATTGGATACTTAACTGTATCTTTCTAAGCTATCACATATCAAAGGAGAG
ATTGAATGCTTTTTTGGAGATCTAGATCATTACTATATGTGTCTCCTATAATCACATCATAGGAGTGAAC
CATAATACACATCTTTGGGTAGGGGAAGGAAAGTATTGTTGACGTACTGATTGATCTGCTTGAGTCAAAT
AATCAGTCATAACAATTCAAGAAAATGCCGGCAGAAAACAAGAAAGTTAGATTCGAAAATACTACTTCAG
ACAAAGGGAAAATTCCTAGTAAAGTTATTAAGAGCTACTACGGAACCATGGACATTAAGAAAATAAATGA
AGGATTATTGGACAGCAAAATATTAAGTGCTTTCAACACAGTAATAGCATTGCTTGGATCTATCGTGATC
ATAGTGATGAATATAATGATCATCCAAAATTACACAAGATCAACAGACAATCAGGCCGTGATCAAAGATG
CGTTGCAGGGTATCCAACAGCAGATCAAAGGGCTTGCTGACAAAATCGGCACAGAGATAGGGCCCAAAGT
ATCACTGATTGACACATCCAGTACCATTACTATCCCAGCTAACATTGGGCTGTTAGGTTCAAAGATCAGC
CAGTCGACTGCAAGTATAAATGAGAATGTGAATGAAAAATGCAAATTCACACTGCCTCCCTTGAAAATCC
ACGAATGTAACATTTCTTGTCCTAACCCACTCCCTTTTAGAGAGTATAGGCCACAGACAGAAGGGGTGAG
CAATCTAGTAGGATTACCTAATAATATTTGCCTGCAAAAGACATCTAATCAGATATTGAAGCCAAAGCTG
ATTTCATACACTTTACCCGTAGTCGGTCAAAGTGGTACCTGTATCACAGACCCATTGCTGGCTATGGACG
AGGGCTATTTTGCATATAGCCACCTGGAAAGAATCGGATCATGTTCAAGAGGGGTCTCCAAACAAAGAAT
AATAGGAGTTGGAGAGGTACTAGACAGAGGTGATGAAGTTCCTTCTTTATTTATGACCAATGTCTGGACC
CCACCAAATCCAAACACCGTTTACCACTGTAGTGCTGTATACAACAATGAATTCTATTATGTACTTTGTG
CAGTGTCAACTGTTGGAGACCCTATTCTGAATAGCACCTACTGGTCCGGATCTCTAATGATGACCCGTCT
AGCTGTGAAACCCAAGAGTAATGGTGGGGGTTACAATCAACATCAACTTGCCCTACGAAGTATCGAGAAA
GGGAGGTATGATAAAGTTATGCCGTATGGACCTTCAGGCATCAAACAGGGTGACACCCTGTATTTTCCTG
CTGTAGGATTTTTGGTCAGGACAGAGTTTAAATACAATGATTCAAATTGTCCCATCACGAAGTGTCAATA
CAGTAAACCTGAAAATTGCAGGCTATCTATGGGGATTAGACCAAACAGCCATTATATCCTTCGATCTGGA
CTATTAAAATACAATCTATCAGATGGGGAGAACCCCAAAGTTGTATTCATTGAAATATCTGATCAAAGAT
TATCTATTGGATCTCCTAGCAAAATCTATGATTCTTTGGGTCAACCTGTTTTCTACCAAGCGTCATTTTC
ATGGGATACTATGATTAAATTTGGAGATGTTCTAACAGTCAACCCTCTGGTTGTCAATTGGCGTAATAAC
ACGGTAATATCAAGACCCGGGCAATCACAATGCCCTAGATTCAATACATGTCCAGAGATCTGCTGGGAAG
GAGTTTATAATGATGCATTCCTAATTGACAGAATCAATTGGATAAGCGCGGGTGTATTCCTTGACAGCAA
TCAGACCGCAGAAAATCCTGTTTTTACTGTATTCAAAGATAATGAAATACTTTATAGGGCACAACTGGCT
TCTGAGGACACCAATGCACAAAAAACAATAACTAATTGTTTTCTCTTGAAGAATAAGATTTGGTGCATAT
CATTGGTTGAGATATATGACACAGGAGACAATGTCATAAGACCCAAACTATTCGCGGTTAAGATACCAGA
GCAATGTACATAAAAATCAACCTCATAATTTAATGGATTGATCTAATATAATGATAATAATCGTACAAAG
ACATGTGATGTAAACAAAATTGTTGTAATTAAATAAGTCCTCAGCTGAATACTTTTTTAAGATTAGCAAT
AGCATGTTTTTCCAGTTATTGGATAGTTGATAATATAATTCTGAAACTGGGTTAATAAATAATCTTGATC
GGTGATCTTTGAGAACAATGATATCATATAGTTCATCAAGTGATAATCAATTCTTTATATGTACACTTTA
GAGTATATTTTGAGACTTAGTATTTTCGGCCCGAATGTTAAATTTAATAGTTCATACATAACCTAAACTC
AAGTTCTAAGCATAATGATAACAATTAATGCGAACTTGTCTTGATGTAAGGAAGATTTGATATTAACTGA
GACTCCACTTGATATAGTAGAGCTGAATCTTGTAAATAAATTATAATGAATAGTTTATTCAAAGATTATC
ATTCATATTAGTGTAAATTAAGAAAA

Protein Sequence : Show Sequence

>NP_112027.1 attachment glycoprotein [Nipah henipavirus]
MPAENKKVRFENTTSDKGKIPSKVIKSYYGTMDIKKINEGLLDSKILSAFNTVIALLGSIVIIVMNIMII
QNYTRSTDNQAVIKDALQGIQQQIKGLADKIGTEIGPKVSLIDTSSTITIPANIGLLGSKISQSTASINE
NVNEKCKFTLPPLKIHECNISCPNPLPFREYRPQTEGVSNLVGLPNNICLQKTSNQILKPKLISYTLPVV
GQSGTCITDPLLAMDEGYFAYSHLERIGSCSRGVSKQRIIGVGEVLDRGDEVPSLFMTNVWTPPNPNTVY
HCSAVYNNEFYYVLCAVSTVGDPILNSTYWSGSLMMTRLAVKPKSNGGGYNQHQLALRSIEKGRYDKVMP
YGPSGIKQGDTLYFPAVGFLVRTEFKYNDSNCPITKCQYSKPENCRLSMGIRPNSHYILRSGLLKYNLSD
GENPKVVFIEISDQRLSIGSPSKIYDSLGQPVFYQASFSWDTMIKFGDVLTVNPLVVNWRNNTVISRPGQ
SQCPRFNTCPEICWEGVYNDAFLIDRINWISAGVFLDSNQTAENPVFTVFKDNEILYRAQLASEDTNAQK
TITNCFLLKNKIWCISLVEIYDTGDNVIRPKLFAVKIPEQCT

Molecule Role : Protective antigen
Molecule Role Annotation : Hamsters were immunized subcutaneously with either 107 PFU of VV-NiV.G or VV-NiV.F expressing the G and F glycoproteins, respectively, or with the two combined. When the VV-NiV.G-vaccinated animals were challenged with Nipah virus 3 months after the last immunization (1,000 PFU/animal intraperitoneally), there was complete protection against mortality (Guillaume et al., 2004).
Related Vaccine(s): ALVAC-NiV-F , ALVAC-NiV-G , NIpah Virus G and F Proteins Subunit Vaccine , Nipah Virus Vaccine rVSVÎ”G-NiVBG , PHV02 , rMV-Ed-G

II. References

1. Guillaume et al., 2004: Guillaume V, Contamin H, Loth P, Georges-Courbot MC, Lefeuvre A, Marianneau P, Chua KB, Lam SK, Buckland R, Deubel V, Wild TF. Nipah virus: vaccination and passive protection studies in a hamster model. Journal of virology. 2004; 78(2); 834-840. [PubMed: 14694115].