VIOLIN: Vaccine Investigation and Online Information Network

G2

General Information

Protegen ID

762

Sequence Strain (Species/Organism)

Trypanosoma cruzi

VO ID

VO_0011299

Taxonomy ID

5693

Molecule Role

Protective antigen

Molecule Role Annotation

The vaccine efficacy of a putative candidate antigen against T. cruzi infection and disease in a mouse model. C57BL/6 mice vaccinated with a T. cruzi G2-encoding plasmid and cytokine (interleukin-12 and granulocyte-macrophage colony-stimulating factor) expression plasmids elicited a strong Th1-type antibody response dominated by immunoglobulin G2b (IgG2b)/IgG1 isotypes. The dominant IgG2b/IgG1 antibody response was maintained after a challenge infection and was associated with 50% control of the acute-phase tissue parasite burden and an almost undetectable level of tissue parasites during the chronic phase, as determined by a sensitive T. cruzi 18S rRNA gene-specific real-time PCR approach (Bhatia and Garg, 2008).

Related Vaccines(s)

T. cruzi DNA prime/Protein-boost vaccine encoding TcG2 and TcG4 , T. cruzi DNA Vaccine encoding G2 Protein , T. cruzi DNA-prime/MVA-boost vaccine encoding TcG2 and TcG4 , T. cruzi vaccine encoding TcG2 and TcG4 , T. cruzi Vaccine TcVac2 , T. cruzi Vaccine TcVac3 encoding TcG2 and TcG4 , T. cruzi Vaccine TcVac4

References

Bhatia and Garg, 2008: Bhatia V, Garg NJ. Previously unrecognized vaccine candidates control Trypanosoma cruzi infection and immunopathology in mice. Clinical and vaccine immunology : CVI. 2008; 15(8); 1158-1164. [PubMed: 18550728].

Gene Information

Gene Name

NCBI Gene ID

3543901

Genbank Accession

AAHK01000586

Chromosome No

Unknown

Gene Starting Position

46754

Gene Ending Position

47725

DNA Sequence

>NW_001849278.1:46754-47725 Trypanosoma cruzi strain CL Brener 1047053517025 genomic scaffold, whole genome shotgun sequence
GATGGCGAGGCGAGCGGCACTGCGATGGTGGCCGGCGTGCGGGCCATTGGATTTATTTTTGAAAAACGAG
GAAGTCAAGAATCCTAGCTTGTGTGTGCATAAGAATGATTTTGGTCAAATGGTGTTGAGTGGCATGGGAG
AATTACATCTTGAAATTGTCATGTCCCTCTTGGAGCACGATTATAAACTAAAGTGTCGACTACTACGTGC
CATCGTAGAATATCGTGAGGTAGTCCTTGAGGCAGTGGAGCTCCTGCATGGTATTGGGATGTACAATGAG
CTCCCATACGTTGAGTGTTCGCTGCGACTCCAGCCACTACTTGAGGAAGATGGCTTCTGCGATCCAGTTC
AATATTGTTCCTTTGTTCTTGACGAGACGTTGACGGAGAAATATCTTGCTGGAGCCGCTGGTTCCCGTAA
TGATCGACGTCGCCGCATGGAGGATCACCACCGTAATGTGAAGGAGGAACTACGAATAAACACCGATGTT
TTTTCTCGCGCAGTCACCGATTGCTCTCACATGGGTCCCCTTGCCGGTTTGCCTCTCCACGGCGTGCGGG
TCGCTTTGCTGGAATTCAAAAAGCTTGGTGGCACGCAGCTGTCGGAGGGCCCTCTACAGCAGGCCGCTCG
TTCTCTTGTATTGGAGCTGTTTCGCTCCGTTTCCAAGGCCAATCTTGCCACGATGGAGCCAATGATGGAG
GTGGAGGTGCACCTCTCGGAGGCAGCCTACATTGGTGGTGTTGTGAGCTCTCTGAGCGAGCGTAAGGCCA
TTGCGGTGGACATTCAAGATGATGGGAGGTCGGTGAAAGCAATCGTCCCGATGCGAAACATAGTTCGCTA
CACAATGGAACTTTGGAAGGCGGTGAAGGGGCATGCCAGTCTGTACGCACGCTTGCACCACTACCGTGTA
ATTGAAGAAAAGGCTGTCCTCTCGCGAATCATGAAGAATTTGGGCATTTACGAAGGCCATTG

Protein Information

Protein Name

protein G2

NCBI Protein GI

71424025

Protein Accession

XP_812654

Protein pI

7.16

Protein Weight

33715.38

Protein Length

323

Protein Sequence

>XP_812654.1 elongation factor G2-like protein, putative [Trypanosoma cruzi]
MARRAALRWWPACGPLDLFLKNEEVKNPSLCVHKNDFGQMVLSGMGELHLEIVMSLLEHDYKLKCRLLRA
IVEYREVVLEAVELLHGIGMYNELPYVECSLRLQPLLEEDGFCDPVQYCSFVLDETLTEKYLAGAAGSRN
DRRRRMEDHHRNVKEELRINTDVFSRAVTDCSHMGPLAGLPLHGVRVALLEFKKLGGTQLSEGPLQQAAR
SLVLELFRSVSKANLATMEPMMEVEVHLSEAAYIGGVVSSLSERKAIAVDIQDDGRSVKAIVPMRNIVRY
TMELWKAVKGHASLYARLHHYRVIEEKAVLSRIMKNLGIYEGH

Epitope Information

IEDB Linear Epitope