Vaccination of mice and guinea pigs with a secA2 mutant significantly increased resistance to M. tuberculosis challenge compared with standard M. bovis bacille Calmette-Guérin vaccination (Hinchey et al., 2007). In another study, lysine auxotrophy combined with deletion of the SecA2 gene made a live attenuated vaccine for Mycobacterium tuberculosis. SCID mice was highly protected from challenge after the vaccination (Hinchey et al., 2011).
COG
COG0653U, under U: Intracellular trafficking, secretion, and vesicular transport
Hinchey et al., 2007: Hinchey J, Lee S, Jeon BY, Basaraba RJ, Venkataswamy MM, Chen B, Chan J, Braunstein M, Orme IM, Derrick SC, Morris SL, Jacobs WR, Porcelli SA. Enhanced priming of adaptive immunity by a proapoptotic mutant of Mycobacterium tuberculosis. The Journal of clinical investigation. 2007 Aug 1; 117(8); 2279-2288. [PubMed: 17671656].
Hinchey et al., 2011: Hinchey J, Jeon BY, Alley H, Chen B, Goldberg M, Derrick S, Morris S, Jacobs WR Jr, Porcelli SA, Lee S. Lysine auxotrophy combined with deletion of the SecA2 gene results in a safe and highly immunogenic candidate live attenuated vaccine for tuberculosis. PloS one. 2011; 6(1); e15857. [PubMed: 21264335].
SecA2; functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond to SecA2; which is non-essential and seems to play a role in secretion of a subset of proteins
Protein Annotation
FUNCTION: Involved in protein export. Interacts with the secY/secE subunits. SecA has a central role in coupling the hydrolysis of ATP to the transfer of pre-secretory proteins across the membrane (By similarity) (UniProt: P66785)
SUBUNIT: Part of the prokaryotic protein translocation apparatus which comprise secA, secB, secD, secE, secF, secG and secY (By similarity) (UniProt: P66785)
