|
|
Calcium phosphate vaccine adjuvant |
|
Vaxjo ID |
80 |
|
Vaccine Adjuvant Name |
Calcium phosphate vaccine adjuvant |
|
Alternative Names |
CaHPO4 |
|
Adjuvant VO ID |
VO_0000131
|
|
Description |
CaHPO4 is a normal constituent of the body and is thus well-tolerated and readily resorbed. According to a study, Unlike AlPO4, CaHPO4 does not induce IgE production in humans and animals (Gupta and Siber, 1994). |
|
Stage of Development |
Licensed |
|
Location Licensed |
Europe |
|
Preparation |
The components of a formulation of 12.5 mM calcium chloride, 12.5 mM dibasic sodium phosphate, and 15.6 mM sodium citrate were mixed together and stirred for 48 h. After a 30-min sonication period, particle size was determined with a Coulter N4Plus submicron particle sizer, and the pH of the mixture was monitored with a pH meter (Fisher,Pittsburgh, Pa.). A preclinical acute toxicity study was performed and was conducted in accordance with the U.S. Food and Drug Administration (He et al., 2000). Calcium phosphate gel (Ca-gel) was prepared by very quickly mixing equimolar solutions of disodium hydrogenphosphate (Kokusan Chemical Works Ltd, Tokyo, Japan) and of calcium chloride (Wako Pure Chemical Industries, Osaka, Japan). Ca-gel, 3 or 9 mg in 1 ml of physiological saline corresponding to ca 1 or 3 mg Ca per millilitre was prepared (He et al., 2000). |
|
Dosage |
100 µg of Calcium phosphate ( CAP) /dose/mouse - Female BALB/c mice (age, 6 to 8 weeks; weight, 20 to 25 g) (He et al., 2000). |
|
Function |
CaHPO4 is a normal constituent of the body and is thus well-tolerated and readily resorbed. According to a study, Unlike AlPO4, CaHPO4 does not induce IgE production in humans and animals (Gupta and Siber, 1994). |
|
Safety |
In France, calcium phosate (CaHPO4) has been used as an adjuvant with diphtheria-tetanus-pertusis (DTP) vaccines form any years and have been found to be safe and efficient in various field trials (Gupta and Siber, 1994). |
| Related Vaccine(s) |
|
| References |
Gupta and Siber, 1994: Gupta RK, Siber GR. Comparison of adjuvant activities of aluminium phosphate, calcium phosphate and stearyl tyrosine for tetanus toxoid. Biologicals : journal of the International Association of Biological Standardization. 1994; 22(1); 53-63. [PubMed: 8068314].
He et al., 2000: He Q, Mitchell AR, Johnson SL, Wagner-Bartak C, Morcol T, Bell SJ. Calcium phosphate nanoparticle adjuvant. Clinical and diagnostic laboratory immunology. 2000; 7(6); 899-903. [PubMed: 11063495].
|
|
