MDP-1 |
|
Vaxjo ID |
366 |
|
Vaccine Adjuvant Name |
MDP-1 |
|
Adjuvant VO ID |
VO_0005653
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|
Description |
adjuvant that induces Th1 response |
|
Stage of Development |
Research |
|
Host Species for Testing |
Chicken |
|
Second Host Species for Testing |
Mouse |
|
Components |
Mycobacterial DNA binding protein 1 (MDP1) is a main cellular protein produced by Mycobacterium bovis |
|
Preparation |
gene was amplified from pcDNA3.1/MDP1 using forward and reverse primers (F: CCC AAG CTT ATG AAC AAA GCA GAG CTC R: AAA GGA TCC CTA TTT GCG ACC CCG) with HindIII and BamHI sites, respectively/.The amplified genes of H5 and MDP1 were digested with HindIII and BamHI to generate cohesive ends for cloning into pcDNA3.1. The digested products were purified by electrophoresis and ligated into pcDNA3.1 using T4 ligation system |
|
Function |
Type: synthetic vaccine adjuvant. Target Receptor: nucleotide-binding oligomerization domain-containing protein 2 (NOD2) | Toll-like receptor 9 (TLR9). Induces Th1-biased immune profile. innate immune activation |
| References |
Jalilian et al., 2010: Jalilian B, Omar AR, Bejo MH, Alitheen NB, Rasoli M, Matsumoto S. Development of avian influenza virus H5 DNA vaccine and MDP-1 gene of Mycobacterium bovis as genetic adjuvant. Genetic vaccines and therapy. 2010; 8; 4. [PubMed: 20497569].
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