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Tumour-derived exosomes (TEXs) |
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Vaxjo ID |
331 |
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Vaccine Adjuvant Name |
Tumour-derived exosomes (TEXs) |
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Description |
The paper studies whether tumor-derived exosomes primed by IRF-1 can boost antitumor immunity. It isolates IRF-1- or IFN-γ-induced exosomes, analyzes IL-15Rα and MHC class I expression, and evaluates their antitumor effects in mice when combined with a CpG adjuvant. |
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Stage of Development |
Research |
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Host Species for Testing |
Mouse |
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Components |
Tumour-derived exosomes (TEXs) |
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Preparation |
Exosomes were isolated via ultracentrifugation from tumour cells infected with IRF-1-expressing adenovirus (IRF-1-Exo) or treated with interferon-纬 (IFN-Exo). |
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Function |
Exosomes from tumor cells expressing IRF-1 or treated with IFN-γ were isolated and analyzed for IL-15Rα and MHC-I. When combined with CpG adjuvant and injected into tumor models, these exosomes were tested for antitumor effects. Tumors were examined by immunofluorescence, and splenocytes were co-cultured with tumor cells to measure IFN-γ^+ and granzyme B^+ CD8^+ T cells by flow cytometry. |
| References |
Yang et al., 2018: Yang MQ, Du Q, Varley PR, Goswami J, Liang Z, Wang R, Li H, Stolz DB, Geller DA. Interferon regulatory factor 1 priming of tumour-derived exosomes enhances the antitumour immune response. British journal of cancer. 2018; 118(1); 62-71. [PubMed: 29112686].
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