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PET Lipid A |
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Vaxjo ID |
285 |
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Vaccine Adjuvant Name |
PET Lipid A |
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Adjuvant VO ID |
VO_0005773
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Description |
PET Lipid A is a synthetic TLR-4 agonist that is used as a potential cancer vaccine adjuvant. |
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Components |
Poly(lactic-co-glycolic acid) particles (size range: 250-600 nm) were successfully formulated to include PET lipid A and/or the model tumor antigen, chicken ovalbumin (OVA).It was shown that particulated PET lipid A had a distinct advantage at promoting secretion of the immune potentiating cytokine, IL-12p70, and upregulating key costimulatory surface proteins, CD86 and CD40, in murine dendritic cells in vitro.In a murine tumor model, involving prophylactic vaccination with various permutations of soluble versus particulated formulations of OVA with or without PET lipid A, modest benefit was observed in terms of OVA-specific cell-mediated immune responses when PET lipid A was delivered in particles.These findings translated into a corresponding trend toward increased survival of mice challenged with OVA-expressing tumor cells (E.G7).In terms of translation of safe adjuvants into the clinic, these results promote the concept of delivering toll-like receptor-4 agonists in particles because doing so improves their adjuvant properties, while decreasing the chances of adverse effects due to off-target uptake by nonphagocytic cells. |
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Structure |
Contains an amine-substituted pentaerythritol instead of a glucosamine unit found in natural lipid A, retaining a hexaacylated chain structure. |
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Preparation |
PET Lipid A is prepared using a double emulsion-solvent evaporation method and is loaded into PLGA particles. |
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Dosage |
In the study, PET Lipid A was administered at a dose of 18 µg. |
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Function |
Development and Evaluation of Biodegradable Particles Coloaded With Antigen and the Toll-Like Receptor Agonist, Pentaerythritol Lipid A, as a Cancer Vaccine |
| References |
Ahmed et al., 2016: Ahmed KK, Geary SM, Salem AK. Development and Evaluation of Biodegradable Particles Coloaded With Antigen and the Toll-Like Receptor Agonist, Pentaerythritol Lipid A, as a Cancer Vaccine. Journal of pharmaceutical sciences. 2016; 105(3); 1173-1179. [PubMed: 26886334].
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