CCL21 and IL1β |
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Vaxjo ID |
283 |
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Vaccine Adjuvant Name |
CCL21 and IL1β |
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Adjuvant VO ID |
VO_0005771
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Description |
This is a recombinant fusion protein of human CCL21 (a chemokine) and a non-inflammatory epitope of IL1β (VQGEESNDK). It was codon-optimized and produced in transgenic tobacco (Nicotiana tabacum). It aims to function as a tumor immunotherapy adjuvant, particularly for breast cancer (MCF7 cell line) |
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Stage of Development |
Research |
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Host Species for Testing |
Mouse |
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Components |
A codon-optimized ccl21/IL1β gene was designed and synthesized from human genes. |
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Structure |
Fusion of CCL21 and IL1β epitope connected by EAAAK linker. 3D structure modeled using Modeller 9.24. Interacts with the CCR7 receptor on T-cells |
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Appearance |
Protein purified from transgenic plant leaves; visualized by SDS-PAGE and western blotting. |
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Preparation |
Synthesized using codon-optimized gene introduced into Agrobacterium tumefaciens, then transferred to tobacco leaves. Expressed under the pBI121 binary vector. Purified using Ni-IDA resin via His-tagSynthesized using codon-optimized gene introduced into Agrobacterium tumefaciens, then transferred to tobacco leaves. Expressed under the pBI121 binary vector. Purified using Ni-IDA resin via His-tag |
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Dosage |
In vitro testing used concentrations of 2.5–10 μg/ml; optimal at 7.5 μg/ml for anti-cancer activity in MCF7 cells |
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Function |
The results demonstrated a decrease in survival rate and metastasization of cancer cells in the presence of CCL21/IL1尾, and IC50 of CCL21 on MCF7 cells was less than that of non-recombinant protein.聽 |
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Safety |
Predicted to be non-allergenic (AlgPred). Stability index supports protein stability. In vitro assays did not report cytotoxicity beyond targeted cancer cells |
| References |
Marashi et al., 2022: Marashi H, Beihaghi M, Chaboksavar M, Khaksar S, Tehrani H, Abiri A. In silico analysis and in planta production of recombinant ccl21/IL1β protein and characterization of its in vitro anti-tumor and immunogenic activity. PloS one. 2022; 17(8); e0261101. [PubMed: 36037155].
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